cacna1eb cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following cacna1eb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1eb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa06575 XM_017351206.2
Latest version!
Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa06576 XM_017351211.2
Latest version!
Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa56377 XM_017351206.1
Latest version!
Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa56378 XM_017351211.1
Latest version!
Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa56379 XM_017351214.1
Latest version!
Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa06575
    Clone ID Related Accession (Same CDS sequence) XM_017351206.2
    Accession Version XM_017351206.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCTCAAC CTGAAGACGC ACCGAAGGAA ATGCTGAGCG CCGAGGGGAA GGCAGAGAGC 
    GAGTCTGCGG GAGAAATAAA GCGGGCTCGA ACCATGGCGC TCTACAACCC CATCCCGGCC
    AAACACAACT GCCTCACCGT CAACAGGTCT CTCTTTATCT TCGCCGAGAA CAATATGATC
    CGAAAATATG CAAAGAGGAT TATAGAGTGG CCTCCGTTTG AGTATATGAT CCTGGCCACC
    ATCATAGCCA ACTGCATCGT TCTGTCCCTG GAGCAGCATC TTCCTGGAGA GGACAAGACA
    CCCATGTCCA AAAGACTGGA GAAGACGGAG CCTTACTTCA TTGGCATCTT CTGTTTCGAG
    GCTGGCATCA AGCTGGTGGC GCTCGGCTTT GTTTTCCATA AGGGCTCCTA TCTGAGAAAC
    GGCTGGAACG TGATGGACTT CATCGTTGTG CTCAGCGGGA TTCTGGCCAC CGCTGGATCT
    CATATGAACA TTCCTGTTGA TCTGAGAACG CTGAGAGCTG TGCGCGTGCT GAGACCCCTG
    AAACTGGTCT CCGGTATCCC CAGTTTACAG ATCGTGCTGA AGTCCATCAT AAAAGCGATG
    GTGCCGCTGC TGCAGATCGG ACTCCTGCTG TTCTTCGCCA TCCTGATGTT CGCCATCATC
    GGTCTGGAGT TTTACAGTGG GAAACTACAC CACACCTGCC TGGCCGACCT GGATATACTC
    GATAATGAGA CGGTGGACTC GTCGGAGGTG GATTTTGCTT GTGGTGTGAG GAAATGTCCT
    GATAAATACA CCTGCAGCGG CTCTTGGATT GGACCCAATG ACGGCATCAC GCAGTTTGAT
    AACATCCTGT TCGCAGTGCT AACAGTGTTT CAGTGCATCA CTATGGAGGG CTGGACGGCG
    GTGCTGTATA ATACTAATGA TGCTCTGGGA CCCACGTGGA ATTGGATATA TTTCATTCCT
    CTCATCATTA TCGGCTCATT CTTTGTGCTC AACCTTGTTT TGGGTGTCCT TTCTGGGGAG
    TTTGCTAAAG AGAGAGAGCG GGTGGAGAAC AGACGTGCTT TCATGAAACT CAGACGACAA
    CAGCAGGTGG AGAGAGAACT GAACGGCTAC CGGGCATGGA TAGACAGAGC AGAGGAGGTC
    ATGCTTCAAG AAGAAAACAA GAATTCTGGG AGGTCTGCAT TAGATGTATT GAAACGAGCC
    ACAAGCAAGA AAAACGCAAG ACGTCGAGGG CCAGGAGAGG AGAAATATTC TGAAATCTCC
    ACAGTTGCAG GTGGATCTCG TCCGAGGGTT GGCATGCGGA CGCCGAGGCG AGGTCCTGCT
    GCGTATCTGA GGCGTAAAGA GCGAATGTTA CGCTTCTCCA TTCGCCGCAT GGTGAAAACC
    GACAGCTTCT ACTGGATAGT GCTCAGTCTG GTGGCGCTCA ACACCATCTC TGTGTCCATC
    GTTCACCACA ACCAGCCCGA ATGGCTCACC GTCATTCAGT ATTACACAGA GTTTGTGTTT
    CTGGGGCTTT TTCTTGCTGA GATGTTTCTG AAAATGTACG GCCTTGGCTT CCGTCTGTAC
    TTCCATTCGT CCTTCAACTG CTTTGACTGT GGGGTGATTG TCGGCAGTAT TTTTGAAGTG
    GTTTGGGGAT TCTTCAGGCC TGGGGTGTCT TTTGGCATCA GTGTCCTCCG AGCTTTACGT
    CTGCTGCGGA TCTTTAAAAT TACCAAGTAT TGGTCGTCCT TGAGGAATTT GGTGGTGTCC
    CTTATGAGCT CCATGAAGTC CATCATCAGT TTGCTCTTCC TCCTATTCCT GTTTATCGTC
    GTCTTCGCTC TTCTCGGCAT GCAGCTGTTT GGCGGCAGGT TCATCTTTGA GGATTACACT
    CCTACTAATT TTGACACATT TCCAGCATCC ATCATGACGG TGTTTCAGAT TCTCACCGGA
    GAGGACTGGA ATGAAGTGAT GTACAACGGG ATTCGTTCTC AAGGAGGAGT GCATTATGGG
    ATGTGGTCTT CCATATACTT CATCGTTTTA ACTTTGTTTG GGAACTACAC ACTGCTCAAT
    GTATTCTTGG CCATTGCTGT GGATAATCTA GCCAATGCAC AGGAACTAAC TAAGGAAGAA
    GAGGAGGAGG AGGAGGAGTT CTTCAACCAG CGCTATAAAA GCAGAGAATT CGGCCTCTCG
    GAAAGGAGAC GGAGACCGTA CCTGTACAGA AAGCGTGCCA TCCACCGAGG GCGACCTTCA
    CCTGCAGAAG CGGAGGAGGA AGCTGCAGGG AAACAAGCAG CGGAAGAACA GCCAGTCAAT
    GCTTTTGCAG GCCGTCGAGA GAGACGCAAA AAAATTAACA TGTCCGTCTG GGAGCAAAGG
    GCCAACCAGC TGCGGAAACG TCGTCAGATG GCCAGCCGTG AGGTACTGTT TGGTGGTCCC
    ACAGAGGACC AGGACACTCC AGGGAGCGAT CATCACCAGC AGCATAGCTT GTCCACTGAA
    ACAAGCCCTT TACACTTGCC AGAATCTCCA ATGTCTGTGG GGATGCCTTT GCCAGAGCCG
    CCTATGTCTA TTACCATTCC TCTGCCTGAG CCTCCAGAGT CTGAGCCGTT GTCGGGATCA
    GGGCTAGAGG AGAGGCCGAG CGCTAATAAC CACCACGCTA ATACCGAAAG GAGACATCGA
    GTGGCGAGGA AGTTTCGAGC TGCTGCTGCT GCAGGGGTGG CGGAGGGTGG GCGACACAAA
    CGCCACAGAC ATCGGGAGTC CAGAACAGAG GCCAGAAATG CAGAGAATCA CCAGCAGTTT
    GAGAGGCAGG CTGAAGACGG TGAAGGTAAA ACAGAGGAAG CTCAATCTAA GCACATGATG
    AACATGTGTG ACCCGGAAAG GCAAATCGAG AATCAAGAGG AGTGCCAAAG GGAGTTTGTG
    TGTGATCTGA CAGGGCTCTC CCAAGCCTCC GGCCATCAGG AGGAAGATCA AGCCGAAAGC
    CAAGAGGCTC AACTTCTGAG GCACAAAGAA GACTTCTCTA CAGAATCTAA ATACATGACA
    GAAGTCTCGG ACATTGAGAA CAACGGCAGC TATCTCAACC AGGATGAGTG TATGACCGAA
    ACCAGCCTAA AACGAGAGTC CTCATCCCAG CCTTTGTTGG AAATGGCACC AATGACTGTC
    AGTTTCAAGG ACGTGGAGGC GTCTCTTTCA GACAAAGCTG ACGATGATGA TGATGATGAT
    ATGGATGATG ATGATGATTA CGAAGACGAT GAGAATGGAG GTGAAAAGTC GACAGCCTCC
    AAACCTGAAA GGCCCGACAG CATGTTCATT TTCAAGAGCA AAAACCCCAT CAGGAGGATC
    TGCCATTATG TGGTGACCCT GCGGTATTTC GAGATGACTA TTCTCCTGGT GATCGTGGCC
    AGCAGTATCG CTCTGGCAGC TGAAGACCCT GTGTGCACCA GTTCAGAGAG AAATAAGGTT
    CTGCGTTACT TTGATTATGT TTTCACTGGA GTTTTCACAT TTGAGATGAT CATAAAGATG
    ATTGACCAGG GCCTCATTCT TCACGATGGG TCGTATTTTC GGGACATGTG GAACATTCTG
    GATTTTATTG TGGTGGTGGG AGCTCTGATT GCGTTTGCAT TGACGAATTT ATTGGGAAAC
    AACAAAGGCC GAGACATTAA GACCATCAAG TCCTTGCGGG TTCTGCGGGT TCTCCGTCCC
    CTCAAGACCA TCAAAAGACT CCCAAAGCTG AAGGCAGTGT TTGATTGCGT GGTCACGTCT
    CTGAAGAACG TCTTCAACAT CCTGATCGTC TACAAGCTCT TCATGTTCAT CTTCGCAGTC
    ATCGCAGTGC AGCTCTTCAA GGGGAAATTC TTCTACTGCA CCGACGGCTC CATGGGCACG
    CAGAAAGAAT GCCAGGGCTA CTACATCGAC TATGGAAGAG ACAGAAAGGA GGTGAAGAAG
    AGGGAATGGA GGAGACACGA GTTTCACTAT GATAACGTCC TCTGGGCTCT TCTAACGCTC
    TTCACCGTGT CCACAGGAGA GGGATGGCCA CAAGTCCTTC AGCACTCGGT GGACGTCACT
    GAAGAAGACC ACGGCCCGAG CAGAGGAAAC CGCATGGAGA TGTCCATTTT CTACGTCATA
    TACTTTGTGG TTTTCCCTTT CTTTTTCGTC AACATTTTTG TAGCTCTCAT CATTATCACA
    TTCCAGGAGC AGGGCGACAA AATGATGGAG GAGTGCAACC TGGAGAAAAA CGAGAGGGCT
    TGCATTGACT TTGCAATCAG TGCCAAACCC CTGACGAGAT ACATGCCTCA GAACAGGCAG
    ACGCTGCAGT ACCGCTTGTG GCACTTTGTG GTGTCGCCGT CTTTCGAGTA CACGGTTCTC
    GTCATGATTG CACTCAACAC TGTAGTGCTC ATGATGAAGT ATTACTCAGC ACCGACGGCC
    TACGATATCG TCCTGAAGCA CCTCAACACG GCCTTCACCG TGCTGTTCTC TCTTGAGTGC
    ATCCTAAAGA TCATGGCCTT CGGGTTTATG AACTATTTCC GGGACACCTG GAACATCTTT
    GATTTCATCA CTGTGCTGGG CAGTATTACC GAAATTGTAG TAGACTTACA GTCAGTAAAC
    ACCATTAATA TGAGTTTCCT GAAGCTTTTC CGGGCAGCAA GACTGATCAA GCTATTGAGA
    CAAGGCTACA CGATACGCAT TCTGCTCTGG ACGTTTGTTC AGTCTTTCAA GGCTCTTCCA
    TATGTTTGTC TTCTTATTGC CATGCTTTTC TTCATTTATG CCATTATCGG CATGCAGGTG
    TTTGGAAATA TTAAACTGAA TGATGAAAGC CATATTAATC AGCACAACAA CTTCAAGACC
    TTCTTTGGTG CCCTGATGCT GCTGTTCAGA AGCGCCACAG GAGAGTCGTG GCAGGAGATC
    ATGCTGTCGT GTCTGGAGGG TAAAGAGTGT GAGCCGGACC CTTCAATAAC ACCACCTTTC
    ACCTCTCCAG ACCACGAGGG CGGCTGCGGC ACCGACTTCG CCTATTTCTA TTTCGTTTCC
    TTCATCTTCT TCAGCTCATT TCTGATGCTG AATCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTATT TGACACGAGA TTCGTCAATT CTTGGACCTC ACCATCTAGA TGAGTTTGTG
    CGCATCTGGG GGGAATATGA CCGCGCAGCA TGTGGCCGGA TTCACTATAA TGCCATGTAT
    GAGATGCTGA CCCACATGTC TCCACCTCTT GGGTTGGGAA AGAAATGTCC TGCAAAAATT
    GCCTACAAGA GACTGGTGCT GATGAACATG CCAGTCGACG AGGACATGAC GGTTCACTTC
    ACATCCACAC TGATGTCTCT GATCCGCACC GCACTGGAGA TCAAGATCGC TAGAGGGGGT
    GAGGATCGGC TACAGCTGGA CATGGAGTTA CAGAAAGAGA TCAGTTTCAT CTGGCCACAT
    CTGTCCCAGA AGACTCTGGA CCTGCTGGTG CCGATAAACA AAGACACTGA CATGACCGTT
    GGAAAGATCT ATGCTTCGAG GATGATCATG GATTTCTTTA AACAGAGCAA AGCCAAGAAG
    CTGAGGCTGC AAGTGGAGGC GCAGAGAGCT GCTGCACTCC CTCTGATATC TCACAGTGCT
    GGATCTGCCC TGACCAGCGG AGGGTTTGTG GCTTTAAGCC CAATTTCACC ACAGGAGCTG
    TTATTGCAGC CCATAACCCA GCGTACAGAC AGCAGTGAAG ACTACGACGC CTCACAGGTT
    GATGAATCAG GGTTGAATGG CGTGTGGGGG GAAGACCGCC CACCTGAACA TGCATTCAGA
    GCCAGACACA AGAGCTACAA AGCTGCAGTG TCCCACTCAG AGCAGACACA GTACATGGAA
    AAGGTGCGAG GACGACCAAA GGAACAACGG CTCTTACTGT CCCCGAAAAA CTCCAACTCA
    AAGTCTCGTT CCCGCTCTCC CAGCGAGGAG CGCGTACATG GGGTCACAAG ACAGGGAAAC
    AGCTCCGAAA GCCCCGTTCC TTCCACCTCA GAGTCCAGCA CCCCGAGTGG AAAACGCAGA
    TTATCCCAAA CCTCCACCTG CTCTCGGCCT CACATCTCCT ACTCGCCTCT AGTGTCCCAC
    ACGCAGTCTT CATCGCAGCC CACTAATAAT AATGAGGAGG ATGAGGAGGA TGATCCAGCG
    GTTCAGCAGA TGTTAGGCTG CTGCAGTACT GCTTTATGGG ATGACAGCGC TGAAGACCAG
    GATGCGAGAG CGAGACAACA AGAAAAATCC CATTTCGAAG TGGATGAATT TTTGGGTTCG
    TCCGTCCGCC GTTATCCATC TGAATCATTC CTCGCTCTCC AAGAGGAAGA GCACAGCCCG
    GATTCTGCCA CAGCAATGGA AACTCTCACT TTTGAGGCGG CGGTGGCCAC CAGCTTAGGC
    CGCGCAAATA CGGTCAGCGC CTCAATGCGC ACTAGATCAC GAATAGGCTG GCAGGTGCCC
    AATGGACACT TCCGGAAGAG GTTGGCGCAG AGTGTTTCTC TTGCTGGGGG CGAAACTCTG
    AGCGATGTAG AGGACGACAA ATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206695.2
    CDS405..6911
    Translation

    Target ORF information:

    RefSeq Version XM_017351206.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCTCAAC CTGAAGACGC ACCGAAGGAA ATGCTGAGCG CCGAGGGGAA GGCAGAGAGC 
    GAGTCTGCGG GAGAAATAAA GCGGGCTCGA ACCATGGCGC TCTACAACCC CATCCCGGCC
    AAACACAACT GCCTCACCGT CAACAGGTCT CTCTTTATCT TCGCCGAGAA CAATATGATC
    CGAAAATATG CAAAGAGGAT TATAGAGTGG CCTCCGTTTG AGTATATGAT CCTGGCCACC
    ATCATAGCCA ACTGCATCGT TCTGTCCCTG GAGCAGCATC TTCCTGGAGA GGACAAGACA
    CCCATGTCCA AAAGACTGGA GAAGACGGAG CCTTACTTCA TTGGCATCTT CTGTTTCGAG
    GCTGGCATCA AGCTGGTGGC GCTCGGCTTT GTTTTCCATA AGGGCTCCTA TCTGAGAAAC
    GGCTGGAACG TGATGGACTT CATCGTTGTG CTCAGCGGGA TTCTGGCCAC CGCTGGATCT
    CATATGAACA TTCCTGTTGA TCTGAGAACG CTGAGAGCTG TGCGCGTGCT GAGACCCCTG
    AAACTGGTCT CCGGTATCCC CAGTTTACAG ATCGTGCTGA AGTCCATCAT AAAAGCGATG
    GTGCCGCTGC TGCAGATCGG ACTCCTGCTG TTCTTCGCCA TCCTGATGTT CGCCATCATC
    GGTCTGGAGT TTTACAGTGG GAAACTACAC CACACCTGCC TGGCCGACCT GGATATACTC
    GATAATGAGA CGGTGGACTC GTCGGAGGTG GATTTTGCTT GTGGTGTGAG GAAATGTCCT
    GATAAATACA CCTGCAGCGG CTCTTGGATT GGACCCAATG ACGGCATCAC GCAGTTTGAT
    AACATCCTGT TCGCAGTGCT AACAGTGTTT CAGTGCATCA CTATGGAGGG CTGGACGGCG
    GTGCTGTATA ATACTAATGA TGCTCTGGGA CCCACGTGGA ATTGGATATA TTTCATTCCT
    CTCATCATTA TCGGCTCATT CTTTGTGCTC AACCTTGTTT TGGGTGTCCT TTCTGGGGAG
    TTTGCTAAAG AGAGAGAGCG GGTGGAGAAC AGACGTGCTT TCATGAAACT CAGACGACAA
    CAGCAGGTGG AGAGAGAACT GAACGGCTAC CGGGCATGGA TAGACAGAGC AGAGGAGGTC
    ATGCTTCAAG AAGAAAACAA GAATTCTGGG AGGTCTGCAT TAGATGTATT GAAACGAGCC
    ACAAGCAAGA AAAACGCAAG ACGTCGAGGG CCAGGAGAGG AGAAATATTC TGAAATCTCC
    ACAGTTGCAG GTGGATCTCG TCCGAGGGTT GGCATGCGGA CGCCGAGGCG AGGTCCTGCT
    GCGTATCTGA GGCGTAAAGA GCGAATGTTA CGCTTCTCCA TTCGCCGCAT GGTGAAAACC
    GACAGCTTCT ACTGGATAGT GCTCAGTCTG GTGGCGCTCA ACACCATCTC TGTGTCCATC
    GTTCACCACA ACCAGCCCGA ATGGCTCACC GTCATTCAGT ATTACACAGA GTTTGTGTTT
    CTGGGGCTTT TTCTTGCTGA GATGTTTCTG AAAATGTACG GCCTTGGCTT CCGTCTGTAC
    TTCCATTCGT CCTTCAACTG CTTTGACTGT GGGGTGATTG TCGGCAGTAT TTTTGAAGTG
    GTTTGGGGAT TCTTCAGGCC TGGGGTGTCT TTTGGCATCA GTGTCCTCCG AGCTTTACGT
    CTGCTGCGGA TCTTTAAAAT TACCAAGTAT TGGTCGTCCT TGAGGAATTT GGTGGTGTCC
    CTTATGAGCT CCATGAAGTC CATCATCAGT TTGCTCTTCC TCCTATTCCT GTTTATCGTC
    GTCTTCGCTC TTCTCGGCAT GCAGCTGTTT GGCGGCAGGT TCATCTTTGA GGATTACACT
    CCTACTAATT TTGACACATT TCCAGCATCC ATCATGACGG TGTTTCAGAT TCTCACCGGA
    GAGGACTGGA ATGAAGTGAT GTACAACGGG ATTCGTTCTC AAGGAGGAGT GCATTATGGG
    ATGTGGTCTT CCATATACTT CATCGTTTTA ACTTTGTTTG GGAACTACAC ACTGCTCAAT
    GTATTCTTGG CCATTGCTGT GGATAATCTA GCCAATGCAC AGGAACTAAC TAAGGAAGAA
    GAGGAGGAGG AGGAGGAGTT CTTCAACCAG CGCTATAAAA GCAGAGAATT CGGCCTCTCG
    GAAAGGAGAC GGAGACCGTA CCTGTACAGA AAGCGTGCCA TCCACCGAGG GCGACCTTCA
    CCTGCAGAAG CGGAGGAGGA AGCTGCAGGG AAACAAGCAG CGGAAGAACA GCCAGTCAAT
    GCTTTTGCAG GCCGTCGAGA GAGACGCAAA AAAATTAACA TGTCCGTCTG GGAGCAAAGG
    GCCAACCAGC TGCGGAAACG TCGTCAGATG GCCAGCCGTG AGGTACTGTT TGGTGGTCCC
    ACAGAGGACC AGGACACTCC AGGGAGCGAT CATCACCAGC AGCATAGCTT GTCCACTGAA
    ACAAGCCCTT TACACTTGCC AGAATCTCCA ATGTCTGTGG GGATGCCTTT GCCAGAGCCG
    CCTATGTCTA TTACCATTCC TCTGCCTGAG CCTCCAGAGT CTGAGCCGTT GTCGGGATCA
    GGGCTAGAGG AGAGGCCGAG CGCTAATAAC CACCACGCTA ATACCGAAAG GAGACATCGA
    GTGGCGAGGA AGTTTCGAGC TGCTGCTGCT GCAGGGGTGG CGGAGGGTGG GCGACACAAA
    CGCCACAGAC ATCGGGAGTC CAGAACAGAG GCCAGAAATG CAGAGAATCA CCAGCAGTTT
    GAGAGGCAGG CTGAAGACGG TGAAGGTAAA ACAGAGGAAG CTCAATCTAA GCACATGATG
    AACATGTGTG ACCCGGAAAG GCAAATCGAG AATCAAGAGG AGTGCCAAAG GGAGTTTGTG
    TGTGATCTGA CAGGGCTCTC CCAAGCCTCC GGCCATCAGG AGGAAGATCA AGCCGAAAGC
    CAAGAGGCTC AACTTCTGAG GCACAAAGAA GACTTCTCTA CAGAATCTAA ATACATGACA
    GAAGTCTCGG ACATTGAGAA CAACGGCAGC TATCTCAACC AGGATGAGTG TATGACCGAA
    ACCAGCCTAA AACGAGAGTC CTCATCCCAG CCTTTGTTGG AAATGGCACC AATGACTGTC
    AGTTTCAAGG ACGTGGAGGC GTCTCTTTCA GACAAAGCTG ACGATGATGA TGATGATGAT
    ATGGATGATG ATGATGATTA CGAAGACGAT GAGAATGGAG GTGAAAAGTC GACAGCCTCC
    AAACCTGAAA GGCCCGACAG CATGTTCATT TTCAAGAGCA AAAACCCCAT CAGGAGGATC
    TGCCATTATG TGGTGACCCT GCGGTATTTC GAGATGACTA TTCTCCTGGT GATCGTGGCC
    AGCAGTATCG CTCTGGCAGC TGAAGACCCT GTGTGCACCA GTTCAGAGAG AAATAAGGTT
    CTGCGTTACT TTGATTATGT TTTCACTGGA GTTTTCACAT TTGAGATGAT CATAAAGATG
    ATTGACCAGG GCCTCATTCT TCACGATGGG TCGTATTTTC GGGACATGTG GAACATTCTG
    GATTTTATTG TGGTGGTGGG AGCTCTGATT GCGTTTGCAT TGACGAATTT ATTGGGAAAC
    AACAAAGGCC GAGACATTAA GACCATCAAG TCCTTGCGGG TTCTGCGGGT TCTCCGTCCC
    CTCAAGACCA TCAAAAGACT CCCAAAGCTG AAGGCAGTGT TTGATTGCGT GGTCACGTCT
    CTGAAGAACG TCTTCAACAT CCTGATCGTC TACAAGCTCT TCATGTTCAT CTTCGCAGTC
    ATCGCAGTGC AGCTCTTCAA GGGGAAATTC TTCTACTGCA CCGACGGCTC CATGGGCACG
    CAGAAAGAAT GCCAGGGCTA CTACATCGAC TATGGAAGAG ACAGAAAGGA GGTGAAGAAG
    AGGGAATGGA GGAGACACGA GTTTCACTAT GATAACGTCC TCTGGGCTCT TCTAACGCTC
    TTCACCGTGT CCACAGGAGA GGGATGGCCA CAAGTCCTTC AGCACTCGGT GGACGTCACT
    GAAGAAGACC ACGGCCCGAG CAGAGGAAAC CGCATGGAGA TGTCCATTTT CTACGTCATA
    TACTTTGTGG TTTTCCCTTT CTTTTTCGTC AACATTTTTG TAGCTCTCAT CATTATCACA
    TTCCAGGAGC AGGGCGACAA AATGATGGAG GAGTGCAACC TGGAGAAAAA CGAGAGGGCT
    TGCATTGACT TTGCAATCAG TGCCAAACCC CTGACGAGAT ACATGCCTCA GAACAGGCAG
    ACGCTGCAGT ACCGCTTGTG GCACTTTGTG GTGTCGCCGT CTTTCGAGTA CACGGTTCTC
    GTCATGATTG CACTCAACAC TGTAGTGCTC ATGATGAAGT ATTACTCAGC ACCGACGGCC
    TACGATATCG TCCTGAAGCA CCTCAACACG GCCTTCACCG TGCTGTTCTC TCTTGAGTGC
    ATCCTAAAGA TCATGGCCTT CGGGTTTATG AACTATTTCC GGGACACCTG GAACATCTTT
    GATTTCATCA CTGTGCTGGG CAGTATTACC GAAATTGTAG TAGACTTACA GTCAGTAAAC
    ACCATTAATA TGAGTTTCCT GAAGCTTTTC CGGGCAGCAA GACTGATCAA GCTATTGAGA
    CAAGGCTACA CGATACGCAT TCTGCTCTGG ACGTTTGTTC AGTCTTTCAA GGCTCTTCCA
    TATGTTTGTC TTCTTATTGC CATGCTTTTC TTCATTTATG CCATTATCGG CATGCAGGTG
    TTTGGAAATA TTAAACTGAA TGATGAAAGC CATATTAATC AGCACAACAA CTTCAAGACC
    TTCTTTGGTG CCCTGATGCT GCTGTTCAGA AGCGCCACAG GAGAGTCGTG GCAGGAGATC
    ATGCTGTCGT GTCTGGAGGG TAAAGAGTGT GAGCCGGACC CTTCAATAAC ACCACCTTTC
    ACCTCTCCAG ACCACGAGGG CGGCTGCGGC ACCGACTTCG CCTATTTCTA TTTCGTTTCC
    TTCATCTTCT TCAGCTCATT TCTGATGCTG AATCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTATT TGACACGAGA TTCGTCAATT CTTGGACCTC ACCATCTAGA TGAGTTTGTG
    CGCATCTGGG GGGAATATGA CCGCGCAGCA TGTGGCCGGA TTCACTATAA TGCCATGTAT
    GAGATGCTGA CCCACATGTC TCCACCTCTT GGGTTGGGAA AGAAATGTCC TGCAAAAATT
    GCCTACAAGA GACTGGTGCT GATGAACATG CCAGTCGACG AGGACATGAC GGTTCACTTC
    ACATCCACAC TGATGTCTCT GATCCGCACC GCACTGGAGA TCAAGATCGC TAGAGGGGGT
    GAGGATCGGC TACAGCTGGA CATGGAGTTA CAGAAAGAGA TCAGTTTCAT CTGGCCACAT
    CTGTCCCAGA AGACTCTGGA CCTGCTGGTG CCGATAAACA AAGACACTGA CATGACCGTT
    GGAAAGATCT ATGCTTCGAG GATGATCATG GATTTCTTTA AACAGAGCAA AGCCAAGAAG
    CTGAGGCTGC AAGTGGAGGC GCAGAGAGCT GCTGCACTCC CTCTGATATC TCACAGTGCT
    GGATCTGCCC TGACCAGCGG AGGGTTTGTG GCTTTAAGCC CAATTTCACC ACAGGAGCTG
    TTATTGCAGC CCATAACCCA GCGTACAGAC AGCAGTGAAG ACTACGACGC CTCACAGGTT
    GATGAATCAG GGTTGAATGG CGTGTGGGGG GAAGACCGCC CACCTGAACA TGCATTCAGA
    GCCAGACACA AGAGCTACAA AGCTGCAGTG TCCCACTCAG AGCAGACACA GTACATGGAA
    AAGGTGCGAG GACGACCAAA GGAACAACGG CTCTTACTGT CCCCGAAAAA CTCCAACTCA
    AAGTCTCGTT CCCGCTCTCC CAGCGAGGAG CGCGTACATG GGGTCACAAG ACAGGGAAAC
    AGCTCCGAAA GCCCCGTTCC TTCCACCTCA GAGTCCAGCA CCCCGAGTGG AAAACGCAGA
    TTATCCCAAA CCTCCACCTG CTCTCGGCCT CACATCTCCT ACTCGCCTCT AGTGTCCCAC
    ACGCAGTCTT CATCGCAGCC CACTAATAAT AATGAGGAGG ATGAGGAGGA TGATCCAGCG
    GTTCAGCAGA TGTTAGGCTG CTGCAGTACT GCTTTATGGG ATGACAGCGC TGAAGACCAG
    GATGCGAGAG CGAGACAACA AGAAAAATCC CATTTCGAAG TGGATGAATT TTTGGGTTCG
    TCCGTCCGCC GTTATCCATC TGAATCATTC CTCGCTCTCC AAGAGGAAGA GCACAGCCCG
    GATTCTGCCA CAGCAATGGA AACTCTCACT TTTGAGGCGG CGGTGGCCAC CAGCTTAGGC
    CGCGCAAATA CGGTCAGCGC CTCAATGCGC ACTAGATCAC GAATAGGCTG GCAGGTGCCC
    AATGGACACT TCCGGAAGAG GTTGGCGCAG AGTGTTTCTC TTGCTGGGGG CGAAACTCTG
    AGCGATGTAG AGGACGACAA ATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06576
    Clone ID Related Accession (Same CDS sequence) XM_017351211.2
    Accession Version XM_017351211.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCTCAAC CTGAAGACGC ACCGAAGGAA ATGCTGAGCG CCGAGGGGAA GGCAGAGAGC 
    GAGTCTGCGG GAGAAATAAA GCGGGCTCGA ACCATGGCGC TCTACAACCC CATCCCGGCC
    AAACACAACT GCCTCACCGT CAACAGGTCT CTCTTTATCT TCGCCGAGAA CAATATGATC
    CGAAAATATG CAAAGAGGAT TATAGAGTGG CCTCCGTTTG AGTATATGAT CCTGGCCACC
    ATCATAGCCA ACTGCATCGT TCTGTCCCTG GAGCAGCATC TTCCTGGAGA GGACAAGACA
    CCCATGTCCA AAAGACTGGA GAAGACGGAG CCTTACTTCA TTGGCATCTT CTGTTTCGAG
    GCTGGCATCA AGCTGGTGGC GCTCGGCTTT GTTTTCCATA AGGGCTCCTA TCTGAGAAAC
    GGCTGGAACG TGATGGACTT CATCGTTGTG CTCAGCGGGA TTCTGGCCAC CGCTGGATCT
    CATATGAACA TTCCTGTTGA TCTGAGAACG CTGAGAGCTG TGCGCGTGCT GAGACCCCTG
    AAACTGGTCT CCGGTATCCC CAGTTTACAG ATCGTGCTGA AGTCCATCAT AAAAGCGATG
    GTGCCGCTGC TGCAGATCGG ACTCCTGCTG TTCTTCGCCA TCCTGATGTT CGCCATCATC
    GGTCTGGAGT TTTACAGTGG GAAACTACAC CACACCTGCC TGGCCGACCT GGATATACTC
    GATAATGAGA CGGTGGACTC GTCGGAGGTG GATTTTGCTT GTGGTGTGAG GAAATGTCCT
    GATAAATACA CCTGCAGCGG CTCTTGGATT GGACCCAATG ACGGCATCAC GCAGTTTGAT
    AACATCCTGT TCGCAGTGCT AACAGTGTTT CAGTGCATCA CTATGGAGGG CTGGACGGCG
    GTGCTGTATA ATACTAATGA TGCTCTGGGA CCCACGTGGA ATTGGATATA TTTCATTCCT
    CTCATCATTA TCGGCTCATT CTTTGTGCTC AACCTTGTTT TGGGTGTCCT TTCTGGGGAG
    TTTGCTAAAG AGAGAGAGCG GGTGGAGAAC AGACGTGCTT TCATGAAACT CAGACGACAA
    CAGCAGGTGG AGAGAGAACT GAACGGCTAC CGGGCATGGA TAGACAGAGC AGAGGAGGTC
    ATGCTTCAAG AAGAAAACAA GAATTCTGGG AGGTCTGCAT TAGATGTATT GAAACGAGCC
    ACAAGCAAGA AAAACGCAAG ACGTCGAGGG CCAGGAGAGG AGAAATATTC TGAAATCTCC
    ACAGTTGCAG GTGGATCTCG TCCGAGGGTT GGCATGCGGA CGCCGAGGCG AGGTCCTGCT
    GCGTATCTGA GGCGTAAAGA GCGAATGTTA CGCTTCTCCA TTCGCCGCAT GGTGAAAACC
    GACAGCTTCT ACTGGATAGT GCTCAGTCTG GTGGCGCTCA ACACCATCTC TGTGTCCATC
    GTTCACCACA ACCAGCCCGA ATGGCTCACC GTCATTCAGT ATTACACAGA GTTTGTGTTT
    CTGGGGCTTT TTCTTGCTGA GATGTTTCTG AAAATGTACG GCCTTGGCTT CCGTCTGTAC
    TTCCATTCGT CCTTCAACTG CTTTGACTGT GGGGTGATTG TCGGCAGTAT TTTTGAAGTG
    GTTTGGGGAT TCTTCAGGCC TGGGGTGTCT TTTGGCATCA GTGTCCTCCG AGCTTTACGT
    CTGCTGCGGA TCTTTAAAAT TACCAAGTAT TGGTCGTCCT TGAGGAATTT GGTGGTGTCC
    CTTATGAGCT CCATGAAGTC CATCATCAGT TTGCTCTTCC TCCTATTCCT GTTTATCGTC
    GTCTTCGCTC TTCTCGGCAT GCAGCTGTTT GGCGGCAGGT TCATCTTTGA GGATTACACT
    CCTACTAATT TTGACACATT TCCAGCATCC ATCATGACGG TGTTTCAGAT TCTCACCGGA
    GAGGACTGGA ATGAAGTGAT GTACAACGGG ATTCGTTCTC AAGGAGGAGT GCATTATGGG
    ATGTGGTCTT CCATATACTT CATCGTTTTA ACTTTGTTTG GGAACTACAC ACTGCTCAAT
    GTATTCTTGG CCATTGCTGT GGATAATCTA GCCAATGCAC AGGAACTAAC TAAGGAAGAA
    GAGGAGGAGG AGGAGGAGTT CTTCAACCAG CGCTATAAAA GCAGAGAATT CGGCCTCTCG
    GAAAGGAGAC GGAGACCGTA CCTGTACAGA AAGCGTGCCA TCCACCGAGG GCGACCTTCA
    CCTGCAGAAG CGGAGGAGGA AGCTGCAGGG AAACAAGCAG CGGAAGAACA GCCAGTCAAT
    GCTTTTGCAG GCCGTCGAGA GAGACGCAAA AAAATTAACA TGTCCGTCTG GGAGCAAAGG
    GCCAACCAGC TGCGGAAACG TCGTCAGATG GCCAGCCGTG AGGTACTGTT TGGTGGTCCC
    ACAGAGGACC AGGACACTCC AGGGAGCGAT CATCACCAGC AGCATAGCTT GTCCACTGAA
    ACAAGCCCTT TACACTTGCC AGAATCTCCA ATGTCTGTGG GGATGCCTTT GCCAGAGCCG
    CCTATGTCTA TTACCATTCC TCTGCCTGAG CCTCCAGAGT CTGAGCCGTT GTCGGGATCA
    GGGCTAGAGG AGAGGCCGAG CGCTAATAAC CACCACGCTA ATACCGAAAG GAGACATCGA
    GTGGCGAGGA AGTTTCGAGC TGCTGCTGCT GCAGGGGTGG CGGAGGGTGG GCGACACAAA
    CGCCACAGAC ATCGGGAGTC CAGAACAGAG GCCAGAAATG CAGAGAATCA CCAGCAGTTT
    GAGAGGCAGG CTGAAGACGG TGAAGGTAAA ACAGAGGAAG CTCAATCTAA GCACATGATG
    AACATGTGTG ACCCGGAAAG GCAAATCGAG AATCAAGAGG AGTGCCAAAG GGAGTTTGTG
    TGTGATCTGA CAGGGCTCTC CCAAGCCTCC GGCCATCAGG AGGAAGATCA AGCCGAAAGC
    CAAGAGGCTC AACTTCTGAG GCACAAAGAA GACTTCTCTA CAGAATCTAA ATACATGACA
    GAAGTCTCGG ACATTGAGAA CAACGGCAGC TATCTCAACC AGGATGAGTG TATGACCGAA
    ACCAGCCTAA AACGAGAGTC CTCATCCCAG CCTTTGTTGG AAATGGCACC AATGACTGTC
    AGTTTCAAGG ACGTGGAGGC GTCTCTTTCA GACAAAGCTG ACGATGATGA TGATGATGAT
    ATGGATGATG ATGATGATTA CGAAGACGAT GAGAATGGAG GTGAAAAGTC GACAGCCTCC
    AAACCTGAAA GGCCCGACAG CATGTTCATT TTCAAGAGCA AAAACCCCAT CAGGAGGATC
    TGCCATTATG TGGTGACCCT GCGGTATTTC GAGATGACTA TTCTCCTGGT GATCGTGGCC
    AGCAGTATCG CTCTGGCAGC TGAAGACCCT GTGTGCACCA GTTCAGAGAG AAATAAGGTT
    CTGCGTTACT TTGATTATGT TTTCACTGGA GTTTTCACAT TTGAGATGAT CATAAAGATG
    ATTGACCAGG GCCTCATTCT TCACGATGGG TCGTATTTTC GGGACATGTG GAACATTCTG
    GATTTTATTG TGGTGGTGGG AGCTCTGATT GCGTTTGCAT TGACAAACAA CAAAGGCCGA
    GACATTAAGA CCATCAAGTC CTTGCGGGTT CTGCGGGTTC TCCGTCCCCT CAAGACCATC
    AAAAGACTCC CAAAGCTGAA GGCAGTGTTT GATTGCGTGG TCACGTCTCT GAAGAACGTC
    TTCAACATCC TGATCGTCTA CAAGCTCTTC ATGTTCATCT TCGCAGTCAT CGCAGTGCAG
    CTCTTCAAGG GGAAATTCTT CTACTGCACC GACGGCTCCA TGGGCACGCA GAAAGAATGC
    CAGGGCTACT ACATCGACTA TGGAAGAGAC AGAAAGGAGG TGAAGAAGAG GGAATGGAGG
    AGACACGAGT TTCACTATGA TAACGTCCTC TGGGCTCTTC TAACGCTCTT CACCGTGTCC
    ACAGGAGAGG GATGGCCACA AGTCCTTCAG CACTCGGTGG ACGTCACTGA AGAAGACCAC
    GGCCCGAGCA GAGGAAACCG CATGGAGATG TCCATTTTCT ACGTCATATA CTTTGTGGTT
    TTCCCTTTCT TTTTCGTCAA CATTTTTGTA GCTCTCATCA TTATCACATT CCAGGAGCAG
    GGCGACAAAA TGATGGAGGA GTGCAACCTG GAGAAAAACG AGAGGGCTTG CATTGACTTT
    GCAATCAGTG CCAAACCCCT GACGAGATAC ATGCCTCAGA ACAGGCAGAC GCTGCAGTAC
    CGCTTGTGGC ACTTTGTGGT GTCGCCGTCT TTCGAGTACA CGGTTCTCGT CATGATTGCA
    CTCAACACTG TAGTGCTCAT GATGAAGTAT TACTCAGCAC CGACGGCCTA CGATATCGTC
    CTGAAGCACC TCAACACGGC CTTCACCGTG CTGTTCTCTC TTGAGTGCAT CCTAAAGATC
    ATGGCCTTCG GGTTTATGAA CTATTTCCGG GACACCTGGA ACATCTTTGA TTTCATCACT
    GTGCTGGGCA GTATTACCGA AATTGTAGTA GACTTACAGT CAGTAAACAC CATTAATATG
    AGTTTCCTGA AGCTTTTCCG GGCAGCAAGA CTGATCAAGC TATTGAGACA AGGCTACACG
    ATACGCATTC TGCTCTGGAC GTTTGTTCAG TCTTTCAAGG CTCTTCCATA TGTTTGTCTT
    CTTATTGCCA TGCTTTTCTT CATTTATGCC ATTATCGGCA TGCAGGTGTT TGGAAATATT
    AAACTGAATG ATGAAAGCCA TATTAATCAG CACAACAACT TCAAGACCTT CTTTGGTGCC
    CTGATGCTGC TGTTCAGAAG CGCCACAGGA GAGTCGTGGC AGGAGATCAT GCTGTCGTGT
    CTGGAGGGTA AAGAGTGTGA GCCGGACCCT TCAATAACAC CACCTTTCAC CTCTCCAGAC
    CACGAGGGCG GCTGCGGCAC CGACTTCGCC TATTTCTATT TCGTTTCCTT CATCTTCTTC
    AGCTCATTTC TGATGCTGAA TCTGTTCGTG GCTGTCATCA TGGACAACTT TGAGTATTTG
    ACACGAGATT CGTCAATTCT TGGACCTCAC CATCTAGATG AGTTTGTGCG CATCTGGGGG
    GAATATGACC GCGCAGCATG TGGCCGGATT CACTATAATG CCATGTATGA GATGCTGACC
    CACATGTCTC CACCTCTTGG GTTGGGAAAG AAATGTCCTG CAAAAATTGC CTACAAGAGA
    CTGGTGCTGA TGAACATGCC AGTCGACGAG GACATGACGG TTCACTTCAC ATCCACACTG
    ATGTCTCTGA TCCGCACCGC ACTGGAGATC AAGATCGCTA GAGGGGGTGA GGATCGGCTA
    CAGCTGGACA TGGAGTTACA GAAAGAGATC AGTTTCATCT GGCCACATCT GTCCCAGAAG
    ACTCTGGACC TGCTGGTGCC GATAAACAAA GACACTGACA TGACCGTTGG AAAGATCTAT
    GCTTCGAGGA TGATCATGGA TTTCTTTAAA CAGAGCAAAG CCAAGAAGCT GAGGCTGCAA
    GTGGAGGCGC AGAGAGCTGC TGCACTCCCT CTGATATCTC ACAGTGCTGG ATCTGCCCTG
    ACCAGCGGAG GGTTTGTGGC TTTAAGCCCA ATTTCACCAC AGGAGCTGTT ATTGCAGCCC
    ATAACCCAGC GTACAGACAG CAGTGAAGAC TACGACGCCT CACAGGTTGA TGAATCAGGG
    TTGAATGGCG TGTGGGGGGA AGACCGCCCA CCTGAACATG CATTCAGAGC CAGACACAAG
    AGCTACAAAG CTGCAGTGTC CCACTCAGAG CAGACACAGT ACATGGAAAA GGTGCGAGGA
    CGACCAAAGG AACAACGGCT CTTACTGTCC CCGAAAAACT CCAACTCAAA GTCTCGTTCC
    CGCTCTCCCA GCGAGGAGCG CGTACATGGG GTCACAAGAC AGGGAAACAG CTCCGAAAGC
    CCCGTTCCTT CCACCTCAGA GTCCAGCACC CCGAGTGGAA AACGCAGATT ATCCCAAACC
    TCCACCTGCT CTCGGCCTCA CATCTCCTAC TCGCCTCTAG TGTCCCACAC GCAGTCTTCA
    TCGCAGCCCA CTAATAATAA TGAGGAGGAT GAGGAGGATG ATCCAGCGGT TCAGCAGATG
    TTAGGCTGCT GCAGTACTGC TTTATGGGAT GACAGCGCTG AAGACCAGGA TGCGAGAGCG
    AGACAACAAG AAAAATCCCA TTTCGAAGTG GATGAATTTT TGGGTTCGTC CGTCCGCCGT
    TATCCATCTG AATCATTCCT CGCTCTCCAA GAGGAAGAGC ACAGCCCGGA TTCTGCCACA
    GCAATGGAAA CTCTCACTTT TGAGGCGGCG GTGGCCACCA GCTTAGGCCG CGCAAATACG
    GTCAGCGCCT CAATGCGCAC TAGATCACGA ATAGGCTGGC AGGTGCCCAA TGGACACTTC
    CGGAAGAGGT TGGCGCAGAG TGTTTCTCTT GCTGGGGGCG AAACTCTGAG CGATGTAGAG
    GACGACAAAT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206700.2
    CDS405..6899
    Translation

    Target ORF information:

    RefSeq Version XM_017351211.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351211.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCTCAAC CTGAAGACGC ACCGAAGGAA ATGCTGAGCG CCGAGGGGAA GGCAGAGAGC 
    GAGTCTGCGG GAGAAATAAA GCGGGCTCGA ACCATGGCGC TCTACAACCC CATCCCGGCC
    AAACACAACT GCCTCACCGT CAACAGGTCT CTCTTTATCT TCGCCGAGAA CAATATGATC
    CGAAAATATG CAAAGAGGAT TATAGAGTGG CCTCCGTTTG AGTATATGAT CCTGGCCACC
    ATCATAGCCA ACTGCATCGT TCTGTCCCTG GAGCAGCATC TTCCTGGAGA GGACAAGACA
    CCCATGTCCA AAAGACTGGA GAAGACGGAG CCTTACTTCA TTGGCATCTT CTGTTTCGAG
    GCTGGCATCA AGCTGGTGGC GCTCGGCTTT GTTTTCCATA AGGGCTCCTA TCTGAGAAAC
    GGCTGGAACG TGATGGACTT CATCGTTGTG CTCAGCGGGA TTCTGGCCAC CGCTGGATCT
    CATATGAACA TTCCTGTTGA TCTGAGAACG CTGAGAGCTG TGCGCGTGCT GAGACCCCTG
    AAACTGGTCT CCGGTATCCC CAGTTTACAG ATCGTGCTGA AGTCCATCAT AAAAGCGATG
    GTGCCGCTGC TGCAGATCGG ACTCCTGCTG TTCTTCGCCA TCCTGATGTT CGCCATCATC
    GGTCTGGAGT TTTACAGTGG GAAACTACAC CACACCTGCC TGGCCGACCT GGATATACTC
    GATAATGAGA CGGTGGACTC GTCGGAGGTG GATTTTGCTT GTGGTGTGAG GAAATGTCCT
    GATAAATACA CCTGCAGCGG CTCTTGGATT GGACCCAATG ACGGCATCAC GCAGTTTGAT
    AACATCCTGT TCGCAGTGCT AACAGTGTTT CAGTGCATCA CTATGGAGGG CTGGACGGCG
    GTGCTGTATA ATACTAATGA TGCTCTGGGA CCCACGTGGA ATTGGATATA TTTCATTCCT
    CTCATCATTA TCGGCTCATT CTTTGTGCTC AACCTTGTTT TGGGTGTCCT TTCTGGGGAG
    TTTGCTAAAG AGAGAGAGCG GGTGGAGAAC AGACGTGCTT TCATGAAACT CAGACGACAA
    CAGCAGGTGG AGAGAGAACT GAACGGCTAC CGGGCATGGA TAGACAGAGC AGAGGAGGTC
    ATGCTTCAAG AAGAAAACAA GAATTCTGGG AGGTCTGCAT TAGATGTATT GAAACGAGCC
    ACAAGCAAGA AAAACGCAAG ACGTCGAGGG CCAGGAGAGG AGAAATATTC TGAAATCTCC
    ACAGTTGCAG GTGGATCTCG TCCGAGGGTT GGCATGCGGA CGCCGAGGCG AGGTCCTGCT
    GCGTATCTGA GGCGTAAAGA GCGAATGTTA CGCTTCTCCA TTCGCCGCAT GGTGAAAACC
    GACAGCTTCT ACTGGATAGT GCTCAGTCTG GTGGCGCTCA ACACCATCTC TGTGTCCATC
    GTTCACCACA ACCAGCCCGA ATGGCTCACC GTCATTCAGT ATTACACAGA GTTTGTGTTT
    CTGGGGCTTT TTCTTGCTGA GATGTTTCTG AAAATGTACG GCCTTGGCTT CCGTCTGTAC
    TTCCATTCGT CCTTCAACTG CTTTGACTGT GGGGTGATTG TCGGCAGTAT TTTTGAAGTG
    GTTTGGGGAT TCTTCAGGCC TGGGGTGTCT TTTGGCATCA GTGTCCTCCG AGCTTTACGT
    CTGCTGCGGA TCTTTAAAAT TACCAAGTAT TGGTCGTCCT TGAGGAATTT GGTGGTGTCC
    CTTATGAGCT CCATGAAGTC CATCATCAGT TTGCTCTTCC TCCTATTCCT GTTTATCGTC
    GTCTTCGCTC TTCTCGGCAT GCAGCTGTTT GGCGGCAGGT TCATCTTTGA GGATTACACT
    CCTACTAATT TTGACACATT TCCAGCATCC ATCATGACGG TGTTTCAGAT TCTCACCGGA
    GAGGACTGGA ATGAAGTGAT GTACAACGGG ATTCGTTCTC AAGGAGGAGT GCATTATGGG
    ATGTGGTCTT CCATATACTT CATCGTTTTA ACTTTGTTTG GGAACTACAC ACTGCTCAAT
    GTATTCTTGG CCATTGCTGT GGATAATCTA GCCAATGCAC AGGAACTAAC TAAGGAAGAA
    GAGGAGGAGG AGGAGGAGTT CTTCAACCAG CGCTATAAAA GCAGAGAATT CGGCCTCTCG
    GAAAGGAGAC GGAGACCGTA CCTGTACAGA AAGCGTGCCA TCCACCGAGG GCGACCTTCA
    CCTGCAGAAG CGGAGGAGGA AGCTGCAGGG AAACAAGCAG CGGAAGAACA GCCAGTCAAT
    GCTTTTGCAG GCCGTCGAGA GAGACGCAAA AAAATTAACA TGTCCGTCTG GGAGCAAAGG
    GCCAACCAGC TGCGGAAACG TCGTCAGATG GCCAGCCGTG AGGTACTGTT TGGTGGTCCC
    ACAGAGGACC AGGACACTCC AGGGAGCGAT CATCACCAGC AGCATAGCTT GTCCACTGAA
    ACAAGCCCTT TACACTTGCC AGAATCTCCA ATGTCTGTGG GGATGCCTTT GCCAGAGCCG
    CCTATGTCTA TTACCATTCC TCTGCCTGAG CCTCCAGAGT CTGAGCCGTT GTCGGGATCA
    GGGCTAGAGG AGAGGCCGAG CGCTAATAAC CACCACGCTA ATACCGAAAG GAGACATCGA
    GTGGCGAGGA AGTTTCGAGC TGCTGCTGCT GCAGGGGTGG CGGAGGGTGG GCGACACAAA
    CGCCACAGAC ATCGGGAGTC CAGAACAGAG GCCAGAAATG CAGAGAATCA CCAGCAGTTT
    GAGAGGCAGG CTGAAGACGG TGAAGGTAAA ACAGAGGAAG CTCAATCTAA GCACATGATG
    AACATGTGTG ACCCGGAAAG GCAAATCGAG AATCAAGAGG AGTGCCAAAG GGAGTTTGTG
    TGTGATCTGA CAGGGCTCTC CCAAGCCTCC GGCCATCAGG AGGAAGATCA AGCCGAAAGC
    CAAGAGGCTC AACTTCTGAG GCACAAAGAA GACTTCTCTA CAGAATCTAA ATACATGACA
    GAAGTCTCGG ACATTGAGAA CAACGGCAGC TATCTCAACC AGGATGAGTG TATGACCGAA
    ACCAGCCTAA AACGAGAGTC CTCATCCCAG CCTTTGTTGG AAATGGCACC AATGACTGTC
    AGTTTCAAGG ACGTGGAGGC GTCTCTTTCA GACAAAGCTG ACGATGATGA TGATGATGAT
    ATGGATGATG ATGATGATTA CGAAGACGAT GAGAATGGAG GTGAAAAGTC GACAGCCTCC
    AAACCTGAAA GGCCCGACAG CATGTTCATT TTCAAGAGCA AAAACCCCAT CAGGAGGATC
    TGCCATTATG TGGTGACCCT GCGGTATTTC GAGATGACTA TTCTCCTGGT GATCGTGGCC
    AGCAGTATCG CTCTGGCAGC TGAAGACCCT GTGTGCACCA GTTCAGAGAG AAATAAGGTT
    CTGCGTTACT TTGATTATGT TTTCACTGGA GTTTTCACAT TTGAGATGAT CATAAAGATG
    ATTGACCAGG GCCTCATTCT TCACGATGGG TCGTATTTTC GGGACATGTG GAACATTCTG
    GATTTTATTG TGGTGGTGGG AGCTCTGATT GCGTTTGCAT TGACAAACAA CAAAGGCCGA
    GACATTAAGA CCATCAAGTC CTTGCGGGTT CTGCGGGTTC TCCGTCCCCT CAAGACCATC
    AAAAGACTCC CAAAGCTGAA GGCAGTGTTT GATTGCGTGG TCACGTCTCT GAAGAACGTC
    TTCAACATCC TGATCGTCTA CAAGCTCTTC ATGTTCATCT TCGCAGTCAT CGCAGTGCAG
    CTCTTCAAGG GGAAATTCTT CTACTGCACC GACGGCTCCA TGGGCACGCA GAAAGAATGC
    CAGGGCTACT ACATCGACTA TGGAAGAGAC AGAAAGGAGG TGAAGAAGAG GGAATGGAGG
    AGACACGAGT TTCACTATGA TAACGTCCTC TGGGCTCTTC TAACGCTCTT CACCGTGTCC
    ACAGGAGAGG GATGGCCACA AGTCCTTCAG CACTCGGTGG ACGTCACTGA AGAAGACCAC
    GGCCCGAGCA GAGGAAACCG CATGGAGATG TCCATTTTCT ACGTCATATA CTTTGTGGTT
    TTCCCTTTCT TTTTCGTCAA CATTTTTGTA GCTCTCATCA TTATCACATT CCAGGAGCAG
    GGCGACAAAA TGATGGAGGA GTGCAACCTG GAGAAAAACG AGAGGGCTTG CATTGACTTT
    GCAATCAGTG CCAAACCCCT GACGAGATAC ATGCCTCAGA ACAGGCAGAC GCTGCAGTAC
    CGCTTGTGGC ACTTTGTGGT GTCGCCGTCT TTCGAGTACA CGGTTCTCGT CATGATTGCA
    CTCAACACTG TAGTGCTCAT GATGAAGTAT TACTCAGCAC CGACGGCCTA CGATATCGTC
    CTGAAGCACC TCAACACGGC CTTCACCGTG CTGTTCTCTC TTGAGTGCAT CCTAAAGATC
    ATGGCCTTCG GGTTTATGAA CTATTTCCGG GACACCTGGA ACATCTTTGA TTTCATCACT
    GTGCTGGGCA GTATTACCGA AATTGTAGTA GACTTACAGT CAGTAAACAC CATTAATATG
    AGTTTCCTGA AGCTTTTCCG GGCAGCAAGA CTGATCAAGC TATTGAGACA AGGCTACACG
    ATACGCATTC TGCTCTGGAC GTTTGTTCAG TCTTTCAAGG CTCTTCCATA TGTTTGTCTT
    CTTATTGCCA TGCTTTTCTT CATTTATGCC ATTATCGGCA TGCAGGTGTT TGGAAATATT
    AAACTGAATG ATGAAAGCCA TATTAATCAG CACAACAACT TCAAGACCTT CTTTGGTGCC
    CTGATGCTGC TGTTCAGAAG CGCCACAGGA GAGTCGTGGC AGGAGATCAT GCTGTCGTGT
    CTGGAGGGTA AAGAGTGTGA GCCGGACCCT TCAATAACAC CACCTTTCAC CTCTCCAGAC
    CACGAGGGCG GCTGCGGCAC CGACTTCGCC TATTTCTATT TCGTTTCCTT CATCTTCTTC
    AGCTCATTTC TGATGCTGAA TCTGTTCGTG GCTGTCATCA TGGACAACTT TGAGTATTTG
    ACACGAGATT CGTCAATTCT TGGACCTCAC CATCTAGATG AGTTTGTGCG CATCTGGGGG
    GAATATGACC GCGCAGCATG TGGCCGGATT CACTATAATG CCATGTATGA GATGCTGACC
    CACATGTCTC CACCTCTTGG GTTGGGAAAG AAATGTCCTG CAAAAATTGC CTACAAGAGA
    CTGGTGCTGA TGAACATGCC AGTCGACGAG GACATGACGG TTCACTTCAC ATCCACACTG
    ATGTCTCTGA TCCGCACCGC ACTGGAGATC AAGATCGCTA GAGGGGGTGA GGATCGGCTA
    CAGCTGGACA TGGAGTTACA GAAAGAGATC AGTTTCATCT GGCCACATCT GTCCCAGAAG
    ACTCTGGACC TGCTGGTGCC GATAAACAAA GACACTGACA TGACCGTTGG AAAGATCTAT
    GCTTCGAGGA TGATCATGGA TTTCTTTAAA CAGAGCAAAG CCAAGAAGCT GAGGCTGCAA
    GTGGAGGCGC AGAGAGCTGC TGCACTCCCT CTGATATCTC ACAGTGCTGG ATCTGCCCTG
    ACCAGCGGAG GGTTTGTGGC TTTAAGCCCA ATTTCACCAC AGGAGCTGTT ATTGCAGCCC
    ATAACCCAGC GTACAGACAG CAGTGAAGAC TACGACGCCT CACAGGTTGA TGAATCAGGG
    TTGAATGGCG TGTGGGGGGA AGACCGCCCA CCTGAACATG CATTCAGAGC CAGACACAAG
    AGCTACAAAG CTGCAGTGTC CCACTCAGAG CAGACACAGT ACATGGAAAA GGTGCGAGGA
    CGACCAAAGG AACAACGGCT CTTACTGTCC CCGAAAAACT CCAACTCAAA GTCTCGTTCC
    CGCTCTCCCA GCGAGGAGCG CGTACATGGG GTCACAAGAC AGGGAAACAG CTCCGAAAGC
    CCCGTTCCTT CCACCTCAGA GTCCAGCACC CCGAGTGGAA AACGCAGATT ATCCCAAACC
    TCCACCTGCT CTCGGCCTCA CATCTCCTAC TCGCCTCTAG TGTCCCACAC GCAGTCTTCA
    TCGCAGCCCA CTAATAATAA TGAGGAGGAT GAGGAGGATG ATCCAGCGGT TCAGCAGATG
    TTAGGCTGCT GCAGTACTGC TTTATGGGAT GACAGCGCTG AAGACCAGGA TGCGAGAGCG
    AGACAACAAG AAAAATCCCA TTTCGAAGTG GATGAATTTT TGGGTTCGTC CGTCCGCCGT
    TATCCATCTG AATCATTCCT CGCTCTCCAA GAGGAAGAGC ACAGCCCGGA TTCTGCCACA
    GCAATGGAAA CTCTCACTTT TGAGGCGGCG GTGGCCACCA GCTTAGGCCG CGCAAATACG
    GTCAGCGCCT CAATGCGCAC TAGATCACGA ATAGGCTGGC AGGTGCCCAA TGGACACTTC
    CGGAAGAGGT TGGCGCAGAG TGTTTCTCTT GCTGGGGGCG AAACTCTGAG CGATGTAGAG
    GACGACAAAT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56377
    Clone ID Related Accession (Same CDS sequence) XM_017351206.1
    Accession Version XM_017351206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGATCCTGG CCACCATCAT AGCCAACTGC ATCGTTCTGT CCCTGGAGCA GCATCTTCCT 
    GGAGAGGACA AGACACCCAT GTCCAAAAGA CTGGAGAAGA CGGAGCCTTA CTTCATTGGC
    ATCTTCTGTT TCGAGGCTGG CATCAAGCTG GTGGCGCTCG GCTTTGTTTT CCATAAGGGC
    TCCTATCTGA GAAACGGCTG GAACGTGATG GACTTCATCG TTGTGCTCAG CGGGATTCTG
    GCCACCGCTG GATCTCATAT GAACATTCCT GTTGATCTGA GAACGCTGAG AGCTGTGCGC
    GTGCTGAGAC CCCTGAAACT GGTCTCCGGT ATCCCCAGTT TACAGATCGT GCTGAAGTCC
    ATCATAAAAG CGATGGTGCC GCTGCTGCAG ATCGGACTCC TGCTGTTCTT CGCCATCCTG
    ATGTTCGCCA TCATCGGTCT GGAGTTTTAC AGTGGGAAAC TACACCACAC CTGCCTGGCC
    GACCTGGATA TACTCGATAA TGAGACGGTG GACTCGTCGG AGGTGGATTT TGCTTGTGGT
    GTGAGGAAAT GTCCTGATAA ATACACCTGC AGCGGCTCTT GGATTGGACC CAATGACGGC
    ATCACGCAGT TTGATAACAT CCTGTTCGCA GTGCTAACAG TGTTTCAGTG CATCACTATG
    GAGGGCTGGA CGGCGGTGCT GTATAATACT AATGATGCTC TGGGACCCAC GTGGAATTGG
    ATATATTTCA TTCCTCTCAT CATTATCGGC TCATTCTTTG TGCTCAACCT TGTTTTGGGT
    GTCCTTTCTG GGGAGTTTGC TAAAGAGAGA GAGCGGGTGG AGAACAGACG TGCTTTCATG
    AAACTCAGAC GACAACAGCA GGTGGAGAGA GAACTGAACG GCTACCGGGC ATGGATAGAC
    AGAGCAGAGG AGGTCATGCT TCAAGAAGAA AACAAGAATT CTGGGAGGTC TGCATTAGAT
    GTATTGAAAC GAGCCACAAG CAAGAAAAAC GCAAGACGTC GAGGGCCAGG AGAGGAGAAA
    TATTCTGAAA TCTCCACAGT TGCAGGTGGA TCTCGTCCGA GGGTTGGCAT GCGGACGCCG
    AGGCGAGGTC CTGCTGCGTA TCTGAGGCGT AAAGAGCGAA TGTTACGCTT CTCCATTCGC
    CGCATGGTGA AAACCGACAG CTTCTACTGG ATAGTGCTCA GTCTGGTGGC GCTCAACACC
    ATCTCTGTGT CCATCGTTCA CCACAACCAG CCCGAATGGC TCACCGTCAT TCAGTATTAC
    ACAGAGTTTG TGTTTCTGGG GCTTTTTCTT GCTGAGATGT TTCTGAAAAT GTACGGCCTT
    GGCTTCCGTC TGTACTTCCA TTCGTCCTTC AACTGCTTTG ACTGTGGGGT GATTGTCGGC
    AGTATTTTTG AAGTGGTTTG GGGATTCTTC AGGCCTGGGG TGTCTTTTGG CATCAGTGTC
    CTCCGAGCTT TACGTCTGCT GCGGATCTTT AAAATTACCA AGTATTGGTC GTCCTTGAGG
    AATTTGGTGG TGTCCCTTAT GAGCTCCATG AAGTCCATCA TCAGTTTGCT CTTCCTCCTA
    TTCCTGTTTA TCGTCGTCTT CGCTCTTCTC GGCATGCAGC TGTTTGGCGG CAGGTTCATC
    TTTGAGGATT ACACTCCTAC TAATTTTGAC ACATTTCCAG CATCCATCAT GACGGTGTTT
    CAGATTCTCA CCGGAGAGGA CTGGAATGAA GTGATGTACA ACGGGATTCG TTCTCAAGGA
    GGAGTGCATT ATGGGATGTG GTCTTCCATA TACTTCATCG TTTTAACTTT GTTTGGGAAC
    TACACACTGC TCAATGTATT CTTGGCCATT GCTGTGGATA ATCTAGCCAA TGCACAGGAA
    CTAACTAAGG AAGAAGAGGA GGAGGAGGAG GAGTTCTTCA ACCAGCGCTA TAAAAGCAGA
    GAATTCGGCC TCTCGGAAAG GAGACGGAGA CCGTACCTGT ACAGAAAGCG TGCCATCCAC
    CGAGGGCGAC CTTCACCTGC AGAAGCGGAG GAGGAAGCTG CAGGGAAACA AGCAGCGGAA
    GAACAGCCAG TCAATGCTTT TGCAGGCCGT CGAGAGAGAC GCAAAAAAAT TAACATGTCC
    GTCTGGGAGC AAAGGGCCAA CCAGCTGCGG AAACGTCGTC AGATGGCCAG CCGTGAGGTA
    CTGTTTGGTG GTCCCACAGA GGACCAGGAC ACTCCAGGGA GCGATCATCA CCAGCAGCAT
    AGCTTGTCCA CTGAAACAAG CCCTTTACAC TTGCCAGAAT CTCCAATGTC TGTGGGGATG
    CCTTTGCCAG AGCCGCCTAT GTCTATTACC ATTCCTCTGC CTGAGCCTCC AGAGTCTGAG
    CCGTTGTCGG GATCAGGGCT AGAGGAGAGG CCGAGCGCTA ATAACCACCA CGCTAATACC
    GAAAGGAGAC ATCGAGTGGC GAGGAAGTTT CGAGCTGCTG CTGCTGCAGG GGTGGCGGAG
    GGTGGGCGAC ACAAACGCCA CAGACATCGG GAGTCCAGAA CAGAGGCCAG AAATGCAGAG
    AATCACCAGC AGTTTGAGAG GCAGGCTGAA GACGGTGAAG GTAAAACAGA GGAAGCTCAA
    TCTAAGCACA TGATGAACAT GTGTGACCCG GAAAGGCAAA TCGAGAATCA AGAGGAGTGC
    CAAAGGGAGT TTGTGTGTGA TCTGACAGGG CTCTCCCAAG CCTCCGGCCA TCAGGAGGAA
    GATCAAGCCG AAAGCCAAGA GGCTCAACTT CTGAGGCACA AAGAAGACTT CTCTACAGAA
    TCTAAATACA TGACAGAAGT CTCGGACATT GAGAACAACG GCAGCTATCT CAACCAGGAT
    GAGTGTATGA CCGAAACCAG CCTAAAACGA GAGTCCTCAT CCCAGCCTTT GTTGGAAATG
    GCACCAATGA CTGTCAGTTT CAAGGACGTG GAGGCGTCTC TTTCAGACAA AGCTGACGAT
    GATGATGATG ATGATATGGA TGATGATGAT GATTACGAAG ACGATGAGAA TGGAGGTGAA
    AAGTCGACAG CCTCCAAACC TGAAAGGCCC GACAGCATGT TCATTTTCAA GAGCAAAAAC
    CCCATCAGGA GGATCTGCCA TTATGTGGTG ACCCTGCGGT ATTTCGAGAT GACTATTCTC
    CTGGTGATCG TGGCCAGCAG TATCGCTCTG GCAGCTGAAG ACCCTGTGTG CACCAGTTCA
    GAGAGAAATA AGGTTCTGCG TTACTTTGAT TATGTTTTCA CTGGAGTTTT CACATTTGAG
    ATGATCATAA AGATGATTGA CCAGGGCCTC ATTCTTCACG ATGGGTCGTA TTTTCGGGAC
    ATGTGGAACA TTCTGGATTT TATTGTGGTG GTGGGAGCTC TGATTGCGTT TGCATTGACG
    AATTTATTGG GAAACAACAA AGGCCGAGAC ATTAAGACCA TCAAGTCCTT GCGGGTTCTG
    CGGGTTCTCC GTCCCCTCAA GACCATCAAA AGACTCCCAA AGCTGAAGGC AGTGTTTGAT
    TGCGTGGTCA CGTCTCTGAA GAACGTCTTC AACATCCTGA TCGTCTACAA GCTCTTCATG
    TTCATCTTCG CAGTCATCGC AGTGCAGCTC TTCAAGGGGA AATTCTTCTA CTGCACCGAC
    GGCTCCATGG GCACGCAGAA AGAATGCCAG GGCTACTACA TCGACTATGG AAGAGACAGA
    AAGGAGGTGA AGAAGAGGGA ATGGAGGAGA CACGAGTTTC ACTATGATAA CGTCCTCTGG
    GCTCTTCTAA CGCTCTTCAC CGTGTCCACA GGAGAGGGAT GGCCACAAGT CCTTCAGCAC
    TCGGTGGACG TCACTGAAGA AGACCACGGC CCGAGCAGAG GAAACCGCAT GGAGATGTCC
    ATTTTCTACG TCATATACTT TGTGGTTTTC CCTTTCTTTT TCGTCAACAT TTTTGTAGCT
    CTCATCATTA TCACATTCCA GGAGCAGGGC GACAAAATGA TGGAGGAGTG CAACCTGGAG
    AAAAACGAGA GGGCTTGCAT TGACTTTGCA ATCAGTGCCA AACCCCTGAC GAGATACATG
    CCTCAGAACA GGCAGACGCT GCAGTACCGC TTGTGGCACT TTGTGGTGTC GCCGTCTTTC
    GAGTACACGG TTCTCGTCAT GATTGCACTC AACACTGTAG TGCTCATGAT GAAGTATTAC
    TCAGCACCGA CGGCCTACGA TATCGTCCTG AAGCACCTCA ACACGGCCTT CACCGTGCTG
    TTCTCTCTTG AGTGCATCCT AAAGATCATG GCCTTCGGGT TTATGAACTA TTTCCGGGAC
    ACCTGGAACA TCTTTGATTT CATCACTGTG CTGGGCAGTA TTACCGAAAT TGTAGTAGAC
    TTACAGTCAG TAAACACCAT TAATATGAGT TTCCTGAAGC TTTTCCGGGC AGCAAGACTG
    ATCAAGCTAT TGAGACAAGG CTACACGATA CGCATTCTGC TCTGGACGTT TGTTCAGTCT
    TTCAAGGCTC TTCCATATGT TTGTCTTCTT ATTGCCATGC TTTTCTTCAT TTATGCCATT
    ATCGGCATGC AGGTGTTTGG AAATATTAAA CTGAATGATG AAAGCCATAT TAATCAGCAC
    AACAACTTCA AGACCTTCTT TGGTGCCCTG ATGCTGCTGT TCAGAAGCGC CACAGGAGAG
    TCGTGGCAGG AGATCATGCT GTCGTGTCTG GAGGGTAAAG AGTGTGAGCC GGACCCTTCA
    ATAACACCAC CTTTCACCTC TCCAGACCAC GAGGGCGGCT GCGGCACCGA CTTCGCCTAT
    TTCTATTTCG TTTCCTTCAT CTTCTTCAGC TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTCATCATGG ACAACTTTGA GTATTTGACA CGAGATTCGT CAATTCTTGG ACCTCACCAT
    CTAGATGAGT TTGTGCGCAT CTGGGGGGAA TATGACCGCG CAGCATGTGG CCGGATTCAC
    TATAATGCCA TGTATGAGAT GCTGACCCAC ATGTCTCCAC CTCTTGGGTT GGGAAAGAAA
    TGTCCTGCAA AAATTGCCTA CAAGAGACTG GTGCTGATGA ACATGCCAGT CGACGAGGAC
    ATGACGGTTC ACTTCACATC CACACTGATG TCTCTGATCC GCACCGCACT GGAGATCAAG
    ATCGCTAGAG GGGGTGAGGA TCGGCTACAG CTGGACATGG AGTTACAGAA AGAGATCAGT
    TTCATCTGGC CACATCTGTC CCAGAAGACT CTGGACCTGC TGGTGCCGAT AAACAAAGAC
    ACTGACATGA CCGTTGGAAA GATCTATGCT TCGAGGATGA TCATGGATTT CTTTAAACAG
    AGCAAAGCCA AGAAGCTGAG GCTGCAAGTG GAGGCGCAGA GAGCTGCTGC ACTCCCTCTG
    ATATCTCACA GTGCTGGATC TGCCCTGACC AGCGGAGGGT TTGTGGCTTT AAGCCCAATT
    TCACCACAGG AGCTGTTATT GCAGCCCATA ACCCAGCGTA CAGACAGCAG TGAAGACTAC
    GACGCCTCAC AGGTTGATGA ATCAGGGTTG AATGGCGTGT GGGGGGAAGA CCGCCCACCT
    GAACATGCAT TCAGAGCCAG ACACAAGAGC TACAAAGCTG CAGTGTCCCA CTCAGAGCAG
    ACACAGTACA TGGAAAAGGT GCGAGGACGA CCAAAGGAAC AACGGCTCTT ACTGTCCCCG
    AAAAACTCCA ACTCAAAGTC TCGTTCCCGC TCTCCCAGCG AGGAGCGCGT ACATGGGGTC
    ACAAGACAGG GAAACAGCTC CGAAAGCCCC GTTCCTTCCA CCTCAGAGTC CAGCACCCCG
    AGTGGAAAAC GCAGATTATC CCAAACCTCC ACCTGCTCTC GGCCTCACAT CTCCTACTCG
    CCTCTAGTGT CCCACACGCA GTCTTCATCG CAGCCCACTA ATAATAATGA GGAGGATGAG
    GAGGATGATC CAGCGGTTCA GCAGATGTTA GGCTGCTGCA GTACTGCTTT ATGGGATGAC
    AGCGCTGAAG ACCAGGATGC GAGAGCGAGA CAACAAGAAA AATCCCATTT CGAAGTGGAT
    GAATTTTTGG GTTCGTCCGT CCGCCGTTAT CCATCTGAAT CATTCCTCGC TCTCCAAGAG
    GAAGAGCACA GCCCGGATTC TGCCACAGCA ATGGAAACTC TCACTTTTGA GGCGGCGGTG
    GCCACCAGCT TAGGCCGCGC AAATACGGTC AGCGCCTCAA TGCGCACTAG ATCACGAATA
    GGCTGGCAGG TGCCCAATGG ACACTTCCGG AAGAGGTTGG CGCAGAGTGT TTCTCTTGCT
    GGGGGCGAAA CTCTGAGCGA TGTAGAGGAC GACAAATACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206695.1
    CDS8..6289
    Translation

    Target ORF information:

    RefSeq Version XM_017351206.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGATCCTGG CCACCATCAT AGCCAACTGC ATCGTTCTGT CCCTGGAGCA GCATCTTCCT 
    GGAGAGGACA AGACACCCAT GTCCAAAAGA CTGGAGAAGA CGGAGCCTTA CTTCATTGGC
    ATCTTCTGTT TCGAGGCTGG CATCAAGCTG GTGGCGCTCG GCTTTGTTTT CCATAAGGGC
    TCCTATCTGA GAAACGGCTG GAACGTGATG GACTTCATCG TTGTGCTCAG CGGGATTCTG
    GCCACCGCTG GATCTCATAT GAACATTCCT GTTGATCTGA GAACGCTGAG AGCTGTGCGC
    GTGCTGAGAC CCCTGAAACT GGTCTCCGGT ATCCCCAGTT TACAGATCGT GCTGAAGTCC
    ATCATAAAAG CGATGGTGCC GCTGCTGCAG ATCGGACTCC TGCTGTTCTT CGCCATCCTG
    ATGTTCGCCA TCATCGGTCT GGAGTTTTAC AGTGGGAAAC TACACCACAC CTGCCTGGCC
    GACCTGGATA TACTCGATAA TGAGACGGTG GACTCGTCGG AGGTGGATTT TGCTTGTGGT
    GTGAGGAAAT GTCCTGATAA ATACACCTGC AGCGGCTCTT GGATTGGACC CAATGACGGC
    ATCACGCAGT TTGATAACAT CCTGTTCGCA GTGCTAACAG TGTTTCAGTG CATCACTATG
    GAGGGCTGGA CGGCGGTGCT GTATAATACT AATGATGCTC TGGGACCCAC GTGGAATTGG
    ATATATTTCA TTCCTCTCAT CATTATCGGC TCATTCTTTG TGCTCAACCT TGTTTTGGGT
    GTCCTTTCTG GGGAGTTTGC TAAAGAGAGA GAGCGGGTGG AGAACAGACG TGCTTTCATG
    AAACTCAGAC GACAACAGCA GGTGGAGAGA GAACTGAACG GCTACCGGGC ATGGATAGAC
    AGAGCAGAGG AGGTCATGCT TCAAGAAGAA AACAAGAATT CTGGGAGGTC TGCATTAGAT
    GTATTGAAAC GAGCCACAAG CAAGAAAAAC GCAAGACGTC GAGGGCCAGG AGAGGAGAAA
    TATTCTGAAA TCTCCACAGT TGCAGGTGGA TCTCGTCCGA GGGTTGGCAT GCGGACGCCG
    AGGCGAGGTC CTGCTGCGTA TCTGAGGCGT AAAGAGCGAA TGTTACGCTT CTCCATTCGC
    CGCATGGTGA AAACCGACAG CTTCTACTGG ATAGTGCTCA GTCTGGTGGC GCTCAACACC
    ATCTCTGTGT CCATCGTTCA CCACAACCAG CCCGAATGGC TCACCGTCAT TCAGTATTAC
    ACAGAGTTTG TGTTTCTGGG GCTTTTTCTT GCTGAGATGT TTCTGAAAAT GTACGGCCTT
    GGCTTCCGTC TGTACTTCCA TTCGTCCTTC AACTGCTTTG ACTGTGGGGT GATTGTCGGC
    AGTATTTTTG AAGTGGTTTG GGGATTCTTC AGGCCTGGGG TGTCTTTTGG CATCAGTGTC
    CTCCGAGCTT TACGTCTGCT GCGGATCTTT AAAATTACCA AGTATTGGTC GTCCTTGAGG
    AATTTGGTGG TGTCCCTTAT GAGCTCCATG AAGTCCATCA TCAGTTTGCT CTTCCTCCTA
    TTCCTGTTTA TCGTCGTCTT CGCTCTTCTC GGCATGCAGC TGTTTGGCGG CAGGTTCATC
    TTTGAGGATT ACACTCCTAC TAATTTTGAC ACATTTCCAG CATCCATCAT GACGGTGTTT
    CAGATTCTCA CCGGAGAGGA CTGGAATGAA GTGATGTACA ACGGGATTCG TTCTCAAGGA
    GGAGTGCATT ATGGGATGTG GTCTTCCATA TACTTCATCG TTTTAACTTT GTTTGGGAAC
    TACACACTGC TCAATGTATT CTTGGCCATT GCTGTGGATA ATCTAGCCAA TGCACAGGAA
    CTAACTAAGG AAGAAGAGGA GGAGGAGGAG GAGTTCTTCA ACCAGCGCTA TAAAAGCAGA
    GAATTCGGCC TCTCGGAAAG GAGACGGAGA CCGTACCTGT ACAGAAAGCG TGCCATCCAC
    CGAGGGCGAC CTTCACCTGC AGAAGCGGAG GAGGAAGCTG CAGGGAAACA AGCAGCGGAA
    GAACAGCCAG TCAATGCTTT TGCAGGCCGT CGAGAGAGAC GCAAAAAAAT TAACATGTCC
    GTCTGGGAGC AAAGGGCCAA CCAGCTGCGG AAACGTCGTC AGATGGCCAG CCGTGAGGTA
    CTGTTTGGTG GTCCCACAGA GGACCAGGAC ACTCCAGGGA GCGATCATCA CCAGCAGCAT
    AGCTTGTCCA CTGAAACAAG CCCTTTACAC TTGCCAGAAT CTCCAATGTC TGTGGGGATG
    CCTTTGCCAG AGCCGCCTAT GTCTATTACC ATTCCTCTGC CTGAGCCTCC AGAGTCTGAG
    CCGTTGTCGG GATCAGGGCT AGAGGAGAGG CCGAGCGCTA ATAACCACCA CGCTAATACC
    GAAAGGAGAC ATCGAGTGGC GAGGAAGTTT CGAGCTGCTG CTGCTGCAGG GGTGGCGGAG
    GGTGGGCGAC ACAAACGCCA CAGACATCGG GAGTCCAGAA CAGAGGCCAG AAATGCAGAG
    AATCACCAGC AGTTTGAGAG GCAGGCTGAA GACGGTGAAG GTAAAACAGA GGAAGCTCAA
    TCTAAGCACA TGATGAACAT GTGTGACCCG GAAAGGCAAA TCGAGAATCA AGAGGAGTGC
    CAAAGGGAGT TTGTGTGTGA TCTGACAGGG CTCTCCCAAG CCTCCGGCCA TCAGGAGGAA
    GATCAAGCCG AAAGCCAAGA GGCTCAACTT CTGAGGCACA AAGAAGACTT CTCTACAGAA
    TCTAAATACA TGACAGAAGT CTCGGACATT GAGAACAACG GCAGCTATCT CAACCAGGAT
    GAGTGTATGA CCGAAACCAG CCTAAAACGA GAGTCCTCAT CCCAGCCTTT GTTGGAAATG
    GCACCAATGA CTGTCAGTTT CAAGGACGTG GAGGCGTCTC TTTCAGACAA AGCTGACGAT
    GATGATGATG ATGATATGGA TGATGATGAT GATTACGAAG ACGATGAGAA TGGAGGTGAA
    AAGTCGACAG CCTCCAAACC TGAAAGGCCC GACAGCATGT TCATTTTCAA GAGCAAAAAC
    CCCATCAGGA GGATCTGCCA TTATGTGGTG ACCCTGCGGT ATTTCGAGAT GACTATTCTC
    CTGGTGATCG TGGCCAGCAG TATCGCTCTG GCAGCTGAAG ACCCTGTGTG CACCAGTTCA
    GAGAGAAATA AGGTTCTGCG TTACTTTGAT TATGTTTTCA CTGGAGTTTT CACATTTGAG
    ATGATCATAA AGATGATTGA CCAGGGCCTC ATTCTTCACG ATGGGTCGTA TTTTCGGGAC
    ATGTGGAACA TTCTGGATTT TATTGTGGTG GTGGGAGCTC TGATTGCGTT TGCATTGACG
    AATTTATTGG GAAACAACAA AGGCCGAGAC ATTAAGACCA TCAAGTCCTT GCGGGTTCTG
    CGGGTTCTCC GTCCCCTCAA GACCATCAAA AGACTCCCAA AGCTGAAGGC AGTGTTTGAT
    TGCGTGGTCA CGTCTCTGAA GAACGTCTTC AACATCCTGA TCGTCTACAA GCTCTTCATG
    TTCATCTTCG CAGTCATCGC AGTGCAGCTC TTCAAGGGGA AATTCTTCTA CTGCACCGAC
    GGCTCCATGG GCACGCAGAA AGAATGCCAG GGCTACTACA TCGACTATGG AAGAGACAGA
    AAGGAGGTGA AGAAGAGGGA ATGGAGGAGA CACGAGTTTC ACTATGATAA CGTCCTCTGG
    GCTCTTCTAA CGCTCTTCAC CGTGTCCACA GGAGAGGGAT GGCCACAAGT CCTTCAGCAC
    TCGGTGGACG TCACTGAAGA AGACCACGGC CCGAGCAGAG GAAACCGCAT GGAGATGTCC
    ATTTTCTACG TCATATACTT TGTGGTTTTC CCTTTCTTTT TCGTCAACAT TTTTGTAGCT
    CTCATCATTA TCACATTCCA GGAGCAGGGC GACAAAATGA TGGAGGAGTG CAACCTGGAG
    AAAAACGAGA GGGCTTGCAT TGACTTTGCA ATCAGTGCCA AACCCCTGAC GAGATACATG
    CCTCAGAACA GGCAGACGCT GCAGTACCGC TTGTGGCACT TTGTGGTGTC GCCGTCTTTC
    GAGTACACGG TTCTCGTCAT GATTGCACTC AACACTGTAG TGCTCATGAT GAAGTATTAC
    TCAGCACCGA CGGCCTACGA TATCGTCCTG AAGCACCTCA ACACGGCCTT CACCGTGCTG
    TTCTCTCTTG AGTGCATCCT AAAGATCATG GCCTTCGGGT TTATGAACTA TTTCCGGGAC
    ACCTGGAACA TCTTTGATTT CATCACTGTG CTGGGCAGTA TTACCGAAAT TGTAGTAGAC
    TTACAGTCAG TAAACACCAT TAATATGAGT TTCCTGAAGC TTTTCCGGGC AGCAAGACTG
    ATCAAGCTAT TGAGACAAGG CTACACGATA CGCATTCTGC TCTGGACGTT TGTTCAGTCT
    TTCAAGGCTC TTCCATATGT TTGTCTTCTT ATTGCCATGC TTTTCTTCAT TTATGCCATT
    ATCGGCATGC AGGTGTTTGG AAATATTAAA CTGAATGATG AAAGCCATAT TAATCAGCAC
    AACAACTTCA AGACCTTCTT TGGTGCCCTG ATGCTGCTGT TCAGAAGCGC CACAGGAGAG
    TCGTGGCAGG AGATCATGCT GTCGTGTCTG GAGGGTAAAG AGTGTGAGCC GGACCCTTCA
    ATAACACCAC CTTTCACCTC TCCAGACCAC GAGGGCGGCT GCGGCACCGA CTTCGCCTAT
    TTCTATTTCG TTTCCTTCAT CTTCTTCAGC TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTCATCATGG ACAACTTTGA GTATTTGACA CGAGATTCGT CAATTCTTGG ACCTCACCAT
    CTAGATGAGT TTGTGCGCAT CTGGGGGGAA TATGACCGCG CAGCATGTGG CCGGATTCAC
    TATAATGCCA TGTATGAGAT GCTGACCCAC ATGTCTCCAC CTCTTGGGTT GGGAAAGAAA
    TGTCCTGCAA AAATTGCCTA CAAGAGACTG GTGCTGATGA ACATGCCAGT CGACGAGGAC
    ATGACGGTTC ACTTCACATC CACACTGATG TCTCTGATCC GCACCGCACT GGAGATCAAG
    ATCGCTAGAG GGGGTGAGGA TCGGCTACAG CTGGACATGG AGTTACAGAA AGAGATCAGT
    TTCATCTGGC CACATCTGTC CCAGAAGACT CTGGACCTGC TGGTGCCGAT AAACAAAGAC
    ACTGACATGA CCGTTGGAAA GATCTATGCT TCGAGGATGA TCATGGATTT CTTTAAACAG
    AGCAAAGCCA AGAAGCTGAG GCTGCAAGTG GAGGCGCAGA GAGCTGCTGC ACTCCCTCTG
    ATATCTCACA GTGCTGGATC TGCCCTGACC AGCGGAGGGT TTGTGGCTTT AAGCCCAATT
    TCACCACAGG AGCTGTTATT GCAGCCCATA ACCCAGCGTA CAGACAGCAG TGAAGACTAC
    GACGCCTCAC AGGTTGATGA ATCAGGGTTG AATGGCGTGT GGGGGGAAGA CCGCCCACCT
    GAACATGCAT TCAGAGCCAG ACACAAGAGC TACAAAGCTG CAGTGTCCCA CTCAGAGCAG
    ACACAGTACA TGGAAAAGGT GCGAGGACGA CCAAAGGAAC AACGGCTCTT ACTGTCCCCG
    AAAAACTCCA ACTCAAAGTC TCGTTCCCGC TCTCCCAGCG AGGAGCGCGT ACATGGGGTC
    ACAAGACAGG GAAACAGCTC CGAAAGCCCC GTTCCTTCCA CCTCAGAGTC CAGCACCCCG
    AGTGGAAAAC GCAGATTATC CCAAACCTCC ACCTGCTCTC GGCCTCACAT CTCCTACTCG
    CCTCTAGTGT CCCACACGCA GTCTTCATCG CAGCCCACTA ATAATAATGA GGAGGATGAG
    GAGGATGATC CAGCGGTTCA GCAGATGTTA GGCTGCTGCA GTACTGCTTT ATGGGATGAC
    AGCGCTGAAG ACCAGGATGC GAGAGCGAGA CAACAAGAAA AATCCCATTT CGAAGTGGAT
    GAATTTTTGG GTTCGTCCGT CCGCCGTTAT CCATCTGAAT CATTCCTCGC TCTCCAAGAG
    GAAGAGCACA GCCCGGATTC TGCCACAGCA ATGGAAACTC TCACTTTTGA GGCGGCGGTG
    GCCACCAGCT TAGGCCGCGC AAATACGGTC AGCGCCTCAA TGCGCACTAG ATCACGAATA
    GGCTGGCAGG TGCCCAATGG ACACTTCCGG AAGAGGTTGG CGCAGAGTGT TTCTCTTGCT
    GGGGGCGAAA CTCTGAGCGA TGTAGAGGAC GACAAATACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56378
    Clone ID Related Accession (Same CDS sequence) XM_017351211.1
    Accession Version XM_017351211.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGATCCTGG CCACCATCAT AGCCAACTGC ATCGTTCTGT CCCTGGAGCA GCATCTTCCT 
    GGAGAGGACA AGACACCCAT GTCCAAAAGA CTGGAGAAGA CGGAGCCTTA CTTCATTGGC
    ATCTTCTGTT TCGAGGCTGG CATCAAGCTG GTGGCGCTCG GCTTTGTTTT CCATAAGGGC
    TCCTATCTGA GAAACGGCTG GAACGTGATG GACTTCATCG TTGTGCTCAG CGGGATTCTG
    GCCACCGCTG GATCTCATAT GAACATTCCT GTTGATCTGA GAACGCTGAG AGCTGTGCGC
    GTGCTGAGAC CCCTGAAACT GGTCTCCGGT ATCCCCAGTT TACAGATCGT GCTGAAGTCC
    ATCATAAAAG CGATGGTGCC GCTGCTGCAG ATCGGACTCC TGCTGTTCTT CGCCATCCTG
    ATGTTCGCCA TCATCGGTCT GGAGTTTTAC AGTGGGAAAC TACACCACAC CTGCCTGGCC
    GACCTGGATA TACTCGATAA TGAGACGGTG GACTCGTCGG AGGTGGATTT TGCTTGTGGT
    GTGAGGAAAT GTCCTGATAA ATACACCTGC AGCGGCTCTT GGATTGGACC CAATGACGGC
    ATCACGCAGT TTGATAACAT CCTGTTCGCA GTGCTAACAG TGTTTCAGTG CATCACTATG
    GAGGGCTGGA CGGCGGTGCT GTATAATACT AATGATGCTC TGGGACCCAC GTGGAATTGG
    ATATATTTCA TTCCTCTCAT CATTATCGGC TCATTCTTTG TGCTCAACCT TGTTTTGGGT
    GTCCTTTCTG GGGAGTTTGC TAAAGAGAGA GAGCGGGTGG AGAACAGACG TGCTTTCATG
    AAACTCAGAC GACAACAGCA GGTGGAGAGA GAACTGAACG GCTACCGGGC ATGGATAGAC
    AGAGCAGAGG AGGTCATGCT TCAAGAAGAA AACAAGAATT CTGGGAGGTC TGCATTAGAT
    GTATTGAAAC GAGCCACAAG CAAGAAAAAC GCAAGACGTC GAGGGCCAGG AGAGGAGAAA
    TATTCTGAAA TCTCCACAGT TGCAGGTGGA TCTCGTCCGA GGGTTGGCAT GCGGACGCCG
    AGGCGAGGTC CTGCTGCGTA TCTGAGGCGT AAAGAGCGAA TGTTACGCTT CTCCATTCGC
    CGCATGGTGA AAACCGACAG CTTCTACTGG ATAGTGCTCA GTCTGGTGGC GCTCAACACC
    ATCTCTGTGT CCATCGTTCA CCACAACCAG CCCGAATGGC TCACCGTCAT TCAGTATTAC
    ACAGAGTTTG TGTTTCTGGG GCTTTTTCTT GCTGAGATGT TTCTGAAAAT GTACGGCCTT
    GGCTTCCGTC TGTACTTCCA TTCGTCCTTC AACTGCTTTG ACTGTGGGGT GATTGTCGGC
    AGTATTTTTG AAGTGGTTTG GGGATTCTTC AGGCCTGGGG TGTCTTTTGG CATCAGTGTC
    CTCCGAGCTT TACGTCTGCT GCGGATCTTT AAAATTACCA AGTATTGGTC GTCCTTGAGG
    AATTTGGTGG TGTCCCTTAT GAGCTCCATG AAGTCCATCA TCAGTTTGCT CTTCCTCCTA
    TTCCTGTTTA TCGTCGTCTT CGCTCTTCTC GGCATGCAGC TGTTTGGCGG CAGGTTCATC
    TTTGAGGATT ACACTCCTAC TAATTTTGAC ACATTTCCAG CATCCATCAT GACGGTGTTT
    CAGATTCTCA CCGGAGAGGA CTGGAATGAA GTGATGTACA ACGGGATTCG TTCTCAAGGA
    GGAGTGCATT ATGGGATGTG GTCTTCCATA TACTTCATCG TTTTAACTTT GTTTGGGAAC
    TACACACTGC TCAATGTATT CTTGGCCATT GCTGTGGATA ATCTAGCCAA TGCACAGGAA
    CTAACTAAGG AAGAAGAGGA GGAGGAGGAG GAGTTCTTCA ACCAGCGCTA TAAAAGCAGA
    GAATTCGGCC TCTCGGAAAG GAGACGGAGA CCGTACCTGT ACAGAAAGCG TGCCATCCAC
    CGAGGGCGAC CTTCACCTGC AGAAGCGGAG GAGGAAGCTG CAGGGAAACA AGCAGCGGAA
    GAACAGCCAG TCAATGCTTT TGCAGGCCGT CGAGAGAGAC GCAAAAAAAT TAACATGTCC
    GTCTGGGAGC AAAGGGCCAA CCAGCTGCGG AAACGTCGTC AGATGGCCAG CCGTGAGGTA
    CTGTTTGGTG GTCCCACAGA GGACCAGGAC ACTCCAGGGA GCGATCATCA CCAGCAGCAT
    AGCTTGTCCA CTGAAACAAG CCCTTTACAC TTGCCAGAAT CTCCAATGTC TGTGGGGATG
    CCTTTGCCAG AGCCGCCTAT GTCTATTACC ATTCCTCTGC CTGAGCCTCC AGAGTCTGAG
    CCGTTGTCGG GATCAGGGCT AGAGGAGAGG CCGAGCGCTA ATAACCACCA CGCTAATACC
    GAAAGGAGAC ATCGAGTGGC GAGGAAGTTT CGAGCTGCTG CTGCTGCAGG GGTGGCGGAG
    GGTGGGCGAC ACAAACGCCA CAGACATCGG GAGTCCAGAA CAGAGGCCAG AAATGCAGAG
    AATCACCAGC AGTTTGAGAG GCAGGCTGAA GACGGTGAAG GTAAAACAGA GGAAGCTCAA
    TCTAAGCACA TGATGAACAT GTGTGACCCG GAAAGGCAAA TCGAGAATCA AGAGGAGTGC
    CAAAGGGAGT TTGTGTGTGA TCTGACAGGG CTCTCCCAAG CCTCCGGCCA TCAGGAGGAA
    GATCAAGCCG AAAGCCAAGA GGCTCAACTT CTGAGGCACA AAGAAGACTT CTCTACAGAA
    TCTAAATACA TGACAGAAGT CTCGGACATT GAGAACAACG GCAGCTATCT CAACCAGGAT
    GAGTGTATGA CCGAAACCAG CCTAAAACGA GAGTCCTCAT CCCAGCCTTT GTTGGAAATG
    GCACCAATGA CTGTCAGTTT CAAGGACGTG GAGGCGTCTC TTTCAGACAA AGCTGACGAT
    GATGATGATG ATGATATGGA TGATGATGAT GATTACGAAG ACGATGAGAA TGGAGGTGAA
    AAGTCGACAG CCTCCAAACC TGAAAGGCCC GACAGCATGT TCATTTTCAA GAGCAAAAAC
    CCCATCAGGA GGATCTGCCA TTATGTGGTG ACCCTGCGGT ATTTCGAGAT GACTATTCTC
    CTGGTGATCG TGGCCAGCAG TATCGCTCTG GCAGCTGAAG ACCCTGTGTG CACCAGTTCA
    GAGAGAAATA AGGTTCTGCG TTACTTTGAT TATGTTTTCA CTGGAGTTTT CACATTTGAG
    ATGATCATAA AGATGATTGA CCAGGGCCTC ATTCTTCACG ATGGGTCGTA TTTTCGGGAC
    ATGTGGAACA TTCTGGATTT TATTGTGGTG GTGGGAGCTC TGATTGCGTT TGCATTGACA
    AACAACAAAG GCCGAGACAT TAAGACCATC AAGTCCTTGC GGGTTCTGCG GGTTCTCCGT
    CCCCTCAAGA CCATCAAAAG ACTCCCAAAG CTGAAGGCAG TGTTTGATTG CGTGGTCACG
    TCTCTGAAGA ACGTCTTCAA CATCCTGATC GTCTACAAGC TCTTCATGTT CATCTTCGCA
    GTCATCGCAG TGCAGCTCTT CAAGGGGAAA TTCTTCTACT GCACCGACGG CTCCATGGGC
    ACGCAGAAAG AATGCCAGGG CTACTACATC GACTATGGAA GAGACAGAAA GGAGGTGAAG
    AAGAGGGAAT GGAGGAGACA CGAGTTTCAC TATGATAACG TCCTCTGGGC TCTTCTAACG
    CTCTTCACCG TGTCCACAGG AGAGGGATGG CCACAAGTCC TTCAGCACTC GGTGGACGTC
    ACTGAAGAAG ACCACGGCCC GAGCAGAGGA AACCGCATGG AGATGTCCAT TTTCTACGTC
    ATATACTTTG TGGTTTTCCC TTTCTTTTTC GTCAACATTT TTGTAGCTCT CATCATTATC
    ACATTCCAGG AGCAGGGCGA CAAAATGATG GAGGAGTGCA ACCTGGAGAA AAACGAGAGG
    GCTTGCATTG ACTTTGCAAT CAGTGCCAAA CCCCTGACGA GATACATGCC TCAGAACAGG
    CAGACGCTGC AGTACCGCTT GTGGCACTTT GTGGTGTCGC CGTCTTTCGA GTACACGGTT
    CTCGTCATGA TTGCACTCAA CACTGTAGTG CTCATGATGA AGTATTACTC AGCACCGACG
    GCCTACGATA TCGTCCTGAA GCACCTCAAC ACGGCCTTCA CCGTGCTGTT CTCTCTTGAG
    TGCATCCTAA AGATCATGGC CTTCGGGTTT ATGAACTATT TCCGGGACAC CTGGAACATC
    TTTGATTTCA TCACTGTGCT GGGCAGTATT ACCGAAATTG TAGTAGACTT ACAGTCAGTA
    AACACCATTA ATATGAGTTT CCTGAAGCTT TTCCGGGCAG CAAGACTGAT CAAGCTATTG
    AGACAAGGCT ACACGATACG CATTCTGCTC TGGACGTTTG TTCAGTCTTT CAAGGCTCTT
    CCATATGTTT GTCTTCTTAT TGCCATGCTT TTCTTCATTT ATGCCATTAT CGGCATGCAG
    GTGTTTGGAA ATATTAAACT GAATGATGAA AGCCATATTA ATCAGCACAA CAACTTCAAG
    ACCTTCTTTG GTGCCCTGAT GCTGCTGTTC AGAAGCGCCA CAGGAGAGTC GTGGCAGGAG
    ATCATGCTGT CGTGTCTGGA GGGTAAAGAG TGTGAGCCGG ACCCTTCAAT AACACCACCT
    TTCACCTCTC CAGACCACGA GGGCGGCTGC GGCACCGACT TCGCCTATTT CTATTTCGTT
    TCCTTCATCT TCTTCAGCTC ATTTCTGATG CTGAATCTGT TCGTGGCTGT CATCATGGAC
    AACTTTGAGT ATTTGACACG AGATTCGTCA ATTCTTGGAC CTCACCATCT AGATGAGTTT
    GTGCGCATCT GGGGGGAATA TGACCGCGCA GCATGTGGCC GGATTCACTA TAATGCCATG
    TATGAGATGC TGACCCACAT GTCTCCACCT CTTGGGTTGG GAAAGAAATG TCCTGCAAAA
    ATTGCCTACA AGAGACTGGT GCTGATGAAC ATGCCAGTCG ACGAGGACAT GACGGTTCAC
    TTCACATCCA CACTGATGTC TCTGATCCGC ACCGCACTGG AGATCAAGAT CGCTAGAGGG
    GGTGAGGATC GGCTACAGCT GGACATGGAG TTACAGAAAG AGATCAGTTT CATCTGGCCA
    CATCTGTCCC AGAAGACTCT GGACCTGCTG GTGCCGATAA ACAAAGACAC TGACATGACC
    GTTGGAAAGA TCTATGCTTC GAGGATGATC ATGGATTTCT TTAAACAGAG CAAAGCCAAG
    AAGCTGAGGC TGCAAGTGGA GGCGCAGAGA GCTGCTGCAC TCCCTCTGAT ATCTCACAGT
    GCTGGATCTG CCCTGACCAG CGGAGGGTTT GTGGCTTTAA GCCCAATTTC ACCACAGGAG
    CTGTTATTGC AGCCCATAAC CCAGCGTACA GACAGCAGTG AAGACTACGA CGCCTCACAG
    GTTGATGAAT CAGGGTTGAA TGGCGTGTGG GGGGAAGACC GCCCACCTGA ACATGCATTC
    AGAGCCAGAC ACAAGAGCTA CAAAGCTGCA GTGTCCCACT CAGAGCAGAC ACAGTACATG
    GAAAAGGTGC GAGGACGACC AAAGGAACAA CGGCTCTTAC TGTCCCCGAA AAACTCCAAC
    TCAAAGTCTC GTTCCCGCTC TCCCAGCGAG GAGCGCGTAC ATGGGGTCAC AAGACAGGGA
    AACAGCTCCG AAAGCCCCGT TCCTTCCACC TCAGAGTCCA GCACCCCGAG TGGAAAACGC
    AGATTATCCC AAACCTCCAC CTGCTCTCGG CCTCACATCT CCTACTCGCC TCTAGTGTCC
    CACACGCAGT CTTCATCGCA GCCCACTAAT AATAATGAGG AGGATGAGGA GGATGATCCA
    GCGGTTCAGC AGATGTTAGG CTGCTGCAGT ACTGCTTTAT GGGATGACAG CGCTGAAGAC
    CAGGATGCGA GAGCGAGACA ACAAGAAAAA TCCCATTTCG AAGTGGATGA ATTTTTGGGT
    TCGTCCGTCC GCCGTTATCC ATCTGAATCA TTCCTCGCTC TCCAAGAGGA AGAGCACAGC
    CCGGATTCTG CCACAGCAAT GGAAACTCTC ACTTTTGAGG CGGCGGTGGC CACCAGCTTA
    GGCCGCGCAA ATACGGTCAG CGCCTCAATG CGCACTAGAT CACGAATAGG CTGGCAGGTG
    CCCAATGGAC ACTTCCGGAA GAGGTTGGCG CAGAGTGTTT CTCTTGCTGG GGGCGAAACT
    CTGAGCGATG TAGAGGACGA CAAATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206700.1
    CDS8..6277
    Translation

    Target ORF information:

    RefSeq Version XM_017351211.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGATCCTGG CCACCATCAT AGCCAACTGC ATCGTTCTGT CCCTGGAGCA GCATCTTCCT 
    GGAGAGGACA AGACACCCAT GTCCAAAAGA CTGGAGAAGA CGGAGCCTTA CTTCATTGGC
    ATCTTCTGTT TCGAGGCTGG CATCAAGCTG GTGGCGCTCG GCTTTGTTTT CCATAAGGGC
    TCCTATCTGA GAAACGGCTG GAACGTGATG GACTTCATCG TTGTGCTCAG CGGGATTCTG
    GCCACCGCTG GATCTCATAT GAACATTCCT GTTGATCTGA GAACGCTGAG AGCTGTGCGC
    GTGCTGAGAC CCCTGAAACT GGTCTCCGGT ATCCCCAGTT TACAGATCGT GCTGAAGTCC
    ATCATAAAAG CGATGGTGCC GCTGCTGCAG ATCGGACTCC TGCTGTTCTT CGCCATCCTG
    ATGTTCGCCA TCATCGGTCT GGAGTTTTAC AGTGGGAAAC TACACCACAC CTGCCTGGCC
    GACCTGGATA TACTCGATAA TGAGACGGTG GACTCGTCGG AGGTGGATTT TGCTTGTGGT
    GTGAGGAAAT GTCCTGATAA ATACACCTGC AGCGGCTCTT GGATTGGACC CAATGACGGC
    ATCACGCAGT TTGATAACAT CCTGTTCGCA GTGCTAACAG TGTTTCAGTG CATCACTATG
    GAGGGCTGGA CGGCGGTGCT GTATAATACT AATGATGCTC TGGGACCCAC GTGGAATTGG
    ATATATTTCA TTCCTCTCAT CATTATCGGC TCATTCTTTG TGCTCAACCT TGTTTTGGGT
    GTCCTTTCTG GGGAGTTTGC TAAAGAGAGA GAGCGGGTGG AGAACAGACG TGCTTTCATG
    AAACTCAGAC GACAACAGCA GGTGGAGAGA GAACTGAACG GCTACCGGGC ATGGATAGAC
    AGAGCAGAGG AGGTCATGCT TCAAGAAGAA AACAAGAATT CTGGGAGGTC TGCATTAGAT
    GTATTGAAAC GAGCCACAAG CAAGAAAAAC GCAAGACGTC GAGGGCCAGG AGAGGAGAAA
    TATTCTGAAA TCTCCACAGT TGCAGGTGGA TCTCGTCCGA GGGTTGGCAT GCGGACGCCG
    AGGCGAGGTC CTGCTGCGTA TCTGAGGCGT AAAGAGCGAA TGTTACGCTT CTCCATTCGC
    CGCATGGTGA AAACCGACAG CTTCTACTGG ATAGTGCTCA GTCTGGTGGC GCTCAACACC
    ATCTCTGTGT CCATCGTTCA CCACAACCAG CCCGAATGGC TCACCGTCAT TCAGTATTAC
    ACAGAGTTTG TGTTTCTGGG GCTTTTTCTT GCTGAGATGT TTCTGAAAAT GTACGGCCTT
    GGCTTCCGTC TGTACTTCCA TTCGTCCTTC AACTGCTTTG ACTGTGGGGT GATTGTCGGC
    AGTATTTTTG AAGTGGTTTG GGGATTCTTC AGGCCTGGGG TGTCTTTTGG CATCAGTGTC
    CTCCGAGCTT TACGTCTGCT GCGGATCTTT AAAATTACCA AGTATTGGTC GTCCTTGAGG
    AATTTGGTGG TGTCCCTTAT GAGCTCCATG AAGTCCATCA TCAGTTTGCT CTTCCTCCTA
    TTCCTGTTTA TCGTCGTCTT CGCTCTTCTC GGCATGCAGC TGTTTGGCGG CAGGTTCATC
    TTTGAGGATT ACACTCCTAC TAATTTTGAC ACATTTCCAG CATCCATCAT GACGGTGTTT
    CAGATTCTCA CCGGAGAGGA CTGGAATGAA GTGATGTACA ACGGGATTCG TTCTCAAGGA
    GGAGTGCATT ATGGGATGTG GTCTTCCATA TACTTCATCG TTTTAACTTT GTTTGGGAAC
    TACACACTGC TCAATGTATT CTTGGCCATT GCTGTGGATA ATCTAGCCAA TGCACAGGAA
    CTAACTAAGG AAGAAGAGGA GGAGGAGGAG GAGTTCTTCA ACCAGCGCTA TAAAAGCAGA
    GAATTCGGCC TCTCGGAAAG GAGACGGAGA CCGTACCTGT ACAGAAAGCG TGCCATCCAC
    CGAGGGCGAC CTTCACCTGC AGAAGCGGAG GAGGAAGCTG CAGGGAAACA AGCAGCGGAA
    GAACAGCCAG TCAATGCTTT TGCAGGCCGT CGAGAGAGAC GCAAAAAAAT TAACATGTCC
    GTCTGGGAGC AAAGGGCCAA CCAGCTGCGG AAACGTCGTC AGATGGCCAG CCGTGAGGTA
    CTGTTTGGTG GTCCCACAGA GGACCAGGAC ACTCCAGGGA GCGATCATCA CCAGCAGCAT
    AGCTTGTCCA CTGAAACAAG CCCTTTACAC TTGCCAGAAT CTCCAATGTC TGTGGGGATG
    CCTTTGCCAG AGCCGCCTAT GTCTATTACC ATTCCTCTGC CTGAGCCTCC AGAGTCTGAG
    CCGTTGTCGG GATCAGGGCT AGAGGAGAGG CCGAGCGCTA ATAACCACCA CGCTAATACC
    GAAAGGAGAC ATCGAGTGGC GAGGAAGTTT CGAGCTGCTG CTGCTGCAGG GGTGGCGGAG
    GGTGGGCGAC ACAAACGCCA CAGACATCGG GAGTCCAGAA CAGAGGCCAG AAATGCAGAG
    AATCACCAGC AGTTTGAGAG GCAGGCTGAA GACGGTGAAG GTAAAACAGA GGAAGCTCAA
    TCTAAGCACA TGATGAACAT GTGTGACCCG GAAAGGCAAA TCGAGAATCA AGAGGAGTGC
    CAAAGGGAGT TTGTGTGTGA TCTGACAGGG CTCTCCCAAG CCTCCGGCCA TCAGGAGGAA
    GATCAAGCCG AAAGCCAAGA GGCTCAACTT CTGAGGCACA AAGAAGACTT CTCTACAGAA
    TCTAAATACA TGACAGAAGT CTCGGACATT GAGAACAACG GCAGCTATCT CAACCAGGAT
    GAGTGTATGA CCGAAACCAG CCTAAAACGA GAGTCCTCAT CCCAGCCTTT GTTGGAAATG
    GCACCAATGA CTGTCAGTTT CAAGGACGTG GAGGCGTCTC TTTCAGACAA AGCTGACGAT
    GATGATGATG ATGATATGGA TGATGATGAT GATTACGAAG ACGATGAGAA TGGAGGTGAA
    AAGTCGACAG CCTCCAAACC TGAAAGGCCC GACAGCATGT TCATTTTCAA GAGCAAAAAC
    CCCATCAGGA GGATCTGCCA TTATGTGGTG ACCCTGCGGT ATTTCGAGAT GACTATTCTC
    CTGGTGATCG TGGCCAGCAG TATCGCTCTG GCAGCTGAAG ACCCTGTGTG CACCAGTTCA
    GAGAGAAATA AGGTTCTGCG TTACTTTGAT TATGTTTTCA CTGGAGTTTT CACATTTGAG
    ATGATCATAA AGATGATTGA CCAGGGCCTC ATTCTTCACG ATGGGTCGTA TTTTCGGGAC
    ATGTGGAACA TTCTGGATTT TATTGTGGTG GTGGGAGCTC TGATTGCGTT TGCATTGACA
    AACAACAAAG GCCGAGACAT TAAGACCATC AAGTCCTTGC GGGTTCTGCG GGTTCTCCGT
    CCCCTCAAGA CCATCAAAAG ACTCCCAAAG CTGAAGGCAG TGTTTGATTG CGTGGTCACG
    TCTCTGAAGA ACGTCTTCAA CATCCTGATC GTCTACAAGC TCTTCATGTT CATCTTCGCA
    GTCATCGCAG TGCAGCTCTT CAAGGGGAAA TTCTTCTACT GCACCGACGG CTCCATGGGC
    ACGCAGAAAG AATGCCAGGG CTACTACATC GACTATGGAA GAGACAGAAA GGAGGTGAAG
    AAGAGGGAAT GGAGGAGACA CGAGTTTCAC TATGATAACG TCCTCTGGGC TCTTCTAACG
    CTCTTCACCG TGTCCACAGG AGAGGGATGG CCACAAGTCC TTCAGCACTC GGTGGACGTC
    ACTGAAGAAG ACCACGGCCC GAGCAGAGGA AACCGCATGG AGATGTCCAT TTTCTACGTC
    ATATACTTTG TGGTTTTCCC TTTCTTTTTC GTCAACATTT TTGTAGCTCT CATCATTATC
    ACATTCCAGG AGCAGGGCGA CAAAATGATG GAGGAGTGCA ACCTGGAGAA AAACGAGAGG
    GCTTGCATTG ACTTTGCAAT CAGTGCCAAA CCCCTGACGA GATACATGCC TCAGAACAGG
    CAGACGCTGC AGTACCGCTT GTGGCACTTT GTGGTGTCGC CGTCTTTCGA GTACACGGTT
    CTCGTCATGA TTGCACTCAA CACTGTAGTG CTCATGATGA AGTATTACTC AGCACCGACG
    GCCTACGATA TCGTCCTGAA GCACCTCAAC ACGGCCTTCA CCGTGCTGTT CTCTCTTGAG
    TGCATCCTAA AGATCATGGC CTTCGGGTTT ATGAACTATT TCCGGGACAC CTGGAACATC
    TTTGATTTCA TCACTGTGCT GGGCAGTATT ACCGAAATTG TAGTAGACTT ACAGTCAGTA
    AACACCATTA ATATGAGTTT CCTGAAGCTT TTCCGGGCAG CAAGACTGAT CAAGCTATTG
    AGACAAGGCT ACACGATACG CATTCTGCTC TGGACGTTTG TTCAGTCTTT CAAGGCTCTT
    CCATATGTTT GTCTTCTTAT TGCCATGCTT TTCTTCATTT ATGCCATTAT CGGCATGCAG
    GTGTTTGGAA ATATTAAACT GAATGATGAA AGCCATATTA ATCAGCACAA CAACTTCAAG
    ACCTTCTTTG GTGCCCTGAT GCTGCTGTTC AGAAGCGCCA CAGGAGAGTC GTGGCAGGAG
    ATCATGCTGT CGTGTCTGGA GGGTAAAGAG TGTGAGCCGG ACCCTTCAAT AACACCACCT
    TTCACCTCTC CAGACCACGA GGGCGGCTGC GGCACCGACT TCGCCTATTT CTATTTCGTT
    TCCTTCATCT TCTTCAGCTC ATTTCTGATG CTGAATCTGT TCGTGGCTGT CATCATGGAC
    AACTTTGAGT ATTTGACACG AGATTCGTCA ATTCTTGGAC CTCACCATCT AGATGAGTTT
    GTGCGCATCT GGGGGGAATA TGACCGCGCA GCATGTGGCC GGATTCACTA TAATGCCATG
    TATGAGATGC TGACCCACAT GTCTCCACCT CTTGGGTTGG GAAAGAAATG TCCTGCAAAA
    ATTGCCTACA AGAGACTGGT GCTGATGAAC ATGCCAGTCG ACGAGGACAT GACGGTTCAC
    TTCACATCCA CACTGATGTC TCTGATCCGC ACCGCACTGG AGATCAAGAT CGCTAGAGGG
    GGTGAGGATC GGCTACAGCT GGACATGGAG TTACAGAAAG AGATCAGTTT CATCTGGCCA
    CATCTGTCCC AGAAGACTCT GGACCTGCTG GTGCCGATAA ACAAAGACAC TGACATGACC
    GTTGGAAAGA TCTATGCTTC GAGGATGATC ATGGATTTCT TTAAACAGAG CAAAGCCAAG
    AAGCTGAGGC TGCAAGTGGA GGCGCAGAGA GCTGCTGCAC TCCCTCTGAT ATCTCACAGT
    GCTGGATCTG CCCTGACCAG CGGAGGGTTT GTGGCTTTAA GCCCAATTTC ACCACAGGAG
    CTGTTATTGC AGCCCATAAC CCAGCGTACA GACAGCAGTG AAGACTACGA CGCCTCACAG
    GTTGATGAAT CAGGGTTGAA TGGCGTGTGG GGGGAAGACC GCCCACCTGA ACATGCATTC
    AGAGCCAGAC ACAAGAGCTA CAAAGCTGCA GTGTCCCACT CAGAGCAGAC ACAGTACATG
    GAAAAGGTGC GAGGACGACC AAAGGAACAA CGGCTCTTAC TGTCCCCGAA AAACTCCAAC
    TCAAAGTCTC GTTCCCGCTC TCCCAGCGAG GAGCGCGTAC ATGGGGTCAC AAGACAGGGA
    AACAGCTCCG AAAGCCCCGT TCCTTCCACC TCAGAGTCCA GCACCCCGAG TGGAAAACGC
    AGATTATCCC AAACCTCCAC CTGCTCTCGG CCTCACATCT CCTACTCGCC TCTAGTGTCC
    CACACGCAGT CTTCATCGCA GCCCACTAAT AATAATGAGG AGGATGAGGA GGATGATCCA
    GCGGTTCAGC AGATGTTAGG CTGCTGCAGT ACTGCTTTAT GGGATGACAG CGCTGAAGAC
    CAGGATGCGA GAGCGAGACA ACAAGAAAAA TCCCATTTCG AAGTGGATGA ATTTTTGGGT
    TCGTCCGTCC GCCGTTATCC ATCTGAATCA TTCCTCGCTC TCCAAGAGGA AGAGCACAGC
    CCGGATTCTG CCACAGCAAT GGAAACTCTC ACTTTTGAGG CGGCGGTGGC CACCAGCTTA
    GGCCGCGCAA ATACGGTCAG CGCCTCAATG CGCACTAGAT CACGAATAGG CTGGCAGGTG
    CCCAATGGAC ACTTCCGGAA GAGGTTGGCG CAGAGTGTTT CTCTTGCTGG GGGCGAAACT
    CTGAGCGATG TAGAGGACGA CAAATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56379
    Clone ID Related Accession (Same CDS sequence) XM_017351214.1
    Accession Version XM_017351214.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATCCTGG CCACCATCAT AGCCAACTGC ATCGTTCTGT CCCTGGAGCA GCATCTTCCT 
    GGAGAGGACA AGACACCCAT GTCCAAAAGA CTGGAGAAGA CGGAGCCTTA CTTCATTGGC
    ATCTTCTGTT TCGAGGCTGG CATCAAGCTG GTGGCGCTCG GCTTTGTTTT CCATAAGGGC
    TCCTATCTGA GAAACGGCTG GAACGTGATG GACTTCATCG TTGTGCTCAG CGGGATTCTG
    GCCACCGCTG GATCTCATAT GAACATTCCT GTTGATCTGA GAACGCTGAG AGCTGTGCGC
    GTGCTGAGAC CCCTGAAACT GGTCTCCGGT ATCCCCAGTT TACAGATCGT GCTGAAGTCC
    ATCATAAAAG CGATGGTGCC GCTGCTGCAG ATCGGACTCC TGCTGTTCTT CGCCATCCTG
    ATGTTCGCCA TCATCGGTCT GGAGTTTTAC AGTGGGAAAC TACACCACAC CTGCCTGGCC
    GACCTGGATA TACTCGATAA TGAGACGGTG GACTCGTCGG AGGTGGATTT TGCTTGTGGT
    GTGAGGAAAT GTCCTGATAA ATACACCTGC AGCGGCTCTT GGATTGGACC CAATGACGGC
    ATCACGCAGT TTGATAACAT CCTGTTCGCA GTGCTAACAG TGTTTCAGTG CATCACTATG
    GAGGGCTGGA CGGCGGTGCT GTATAATACT AATGATGCTC TGGGACCCAC GTGGAATTGG
    ATATATTTCA TTCCTCTCAT CATTATCGGC TCATTCTTTG TGCTCAACCT TGTTTTGGGT
    GTCCTTTCTG GGGAGTTTGC TAAAGAGAGA GAGCGGGTGG AGAACAGACG TGCTTTCATG
    AAACTCAGAC GACAACAGCA GGTGGAGAGA GAACTGAACG GCTACCGGGC ATGGATAGAC
    AGAGCAGAGG AGGTCATGCT TCAAGAAGAA AACAAGAATT CTGGGAGGTC TGCATTAGAT
    GTATTGAAAC GAGCCACAAG CAAGAAAAAC GCAAGACGTC GAGGGCCAGG AGAGGAGAAA
    TATTCTGAAA TCTCCACAGT TGCAGGTGGA TCTCGTCCGA GGGTTGGCAT GCGGACGCCG
    AGGCGAGGTC CTGCTGCGTA TCTGAGGCGT AAAGAGCGAA TGTTACGCTT CTCCATTCGC
    CGCATGGTGA AAACCGACAG CTTCTACTGG ATAGTGCTCA GTCTGGTGGC GCTCAACACC
    ATCTCTGTGT CCATCGTTCA CCACAACCAG CCCGAATGGC TCACCGTCAT TCAGTATTAC
    ACAGAGTTTG TGTTTCTGGG GCTTTTTCTT GCTGAGATGT TTCTGAAAAT GTACGGCCTT
    GGCTTCCGTC TGTACTTCCA TTCGTCCTTC AACTGCTTTG ACTGTGGGGT GATTGTCGGC
    AGTATTTTTG AAGTGGTTTG GGGATTCTTC AGGCCTGGGG TGTCTTTTGG CATCAGTGTC
    CTCCGAGCTT TACGTCTGCT GCGGATCTTT AAAATTACCA AGTATTGGTC GTCCTTGAGG
    AATTTGGTGG TGTCCCTTAT GAGCTCCATG AAGTCCATCA TCAGTTTGCT CTTCCTCCTA
    TTCCTGTTTA TCGTCGTCTT CGCTCTTCTC GGCATGCAGC TGTTTGGCGG CAGGTTCATC
    TTTGAGGATT ACACTCCTAC TAATTTTGAC ACATTTCCAG CATCCATCAT GACGGTGTTT
    CAGATTCTCA CCGGAGAGGA CTGGAATGAA GTGATGTACA ACGGGATTCG TTCTCAAGGA
    GGAGTGCATT ATGGGATGTG GTCTTCCATA TACTTCATCG TTTTAACTTT GTTTGGGAAC
    TACACACTGC TCAATGTATT CTTGGCCATT GCTGTGGATA ATCTAGCCAA TGCACAGGAA
    CTAACTAAGG AAGAAGAGGA GGAGGAGGAG GAGTTCTTCA ACCAGCGCTA TAAAAGCAGA
    GAATTCGGCC TCTCGGAAAG GAGACGGAGA CCGTACCTGT ACAGAAAGCG TGCCATCCAC
    CGAGGGCGAC CTTCACCTGC AGAAGCGGAG GAGGAAGCTG CAGGGAAACA AGCAGCGGAA
    GAACAGCCAG TCAATGCTTT TGCAGGCCGT CGAGAGAGAC GCAAAAAAAT TAACATGTCC
    GTCTGGGAGC AAAGGGCCAA CCAGCTGCGG AAACGTCGTC AGATGGCCAG CCGTGAGGTA
    CTGTTTGGTG GTCCCACAGA GGACCAGGAC ACTCCAGGGA GCGATCATCA CCAGCAGCAT
    AGCTTGTCCA CTGAAACAAG CCCTTTACAC TTGCCAGAAT CTCCAATGTC TGTGGGGATG
    CCTTTGCCAG AGCCGCCTAT GTCTATTACC ATTCCTCTGC CTGAGCCTCC AGAGTCTGAG
    CCGTTGTCGG GATCAGGGCT AGAGGAGAGG CCGAGCGCTA ATAACCACCA CGCTAATACC
    GAAAGGAGAC ATCGAGTGGC GAGGAAGTTT CGAGCTGCTG CTGCTGCAGG GGTGGCGGAG
    GGTGGGCGAC ACAAACGCCA CAGACATCGG GAGTCCAGAA CAGAGGCCAG AAATGCAGAG
    AATCACCAGC AGTTTGAGAG GCAGGCTGAA GACGGTGAAG GTAAAACAGA GGAAGCTCAA
    TCTAAGCACA TGATGAACAT GTGTGACCCG GAAAGGCAAA TCGAGAATCA AGAGGAGTGC
    CAAAGGGAGT TTGTGTGTGA TCTGACAGGG CTCTCCCAAG CCTCCGGCCA TCAGGAGGAA
    GATCAAGCCG AAAGCCAAGA GGCTCAACTT CTGAGGCACA AAGAAGACTT CTCTACAGAA
    TCTAAATACA TGACAGAAGT CTCGGACATT GAGAACAACG GCAGCTATCT CAACCAGGAT
    GAGTGTATGA CCGAAACCAG CCTAAAACGA GAGTCCTCAT CCCAGCCTTT GTTGGAAATG
    GCACCAATGA CTGTCAGTTT CAAGGACGTG GAGGCGTCTC TTTCAGACAA AGCTGACGAT
    GATGATGATG ATGATATGGA TGATGATGAT GATTACGAAG ACGATGAGAA TGGAGGTGAA
    AAGTCGACAG CCTCCAAACC TGAAAGGCCC GACAGCATGT TCATTTTCAA GAGCAAAAAC
    CCCATCAGGA GGATCTGCCA TTATGTGGTG ACCCTGCGGT ATTTCGAGAT GACTATTCTC
    CTGGTGATCG TGGCCAGCAG TATCGCTCTG GCAGCTGAAG ACCCTGTGTG CACCAGTTCA
    GAGAGAAATA AGGTTCTGCG TTACTTTGAT TATGTTTTCA CTGGAGTTTT CACATTTGAG
    ATGATCATAA AGATGATTGA CCAGGGCCTC ATTCTTCACG ATGGGTCGTA TTTTCGGGAC
    ATGTGGAACA TTCTGGATTT TATTGTGGTG GTGGGAGCTC TGATTGCGTT TGCATTGACG
    AATTTATTGG GAAACAACAA AGGCCGAGAC ATTAAGACCA TCAAGTCCTT GCGGGTTCTG
    CGGGTTCTCC GTCCCCTCAA GACCATCAAA AGACTCCCAA AGCTGAAGGC AGTGTTTGAT
    TGCGTGGTCA CGTCTCTGAA GAACGTCTTC AACATCCTGA TCGTCTACAA GCTCTTCATG
    TTCATCTTCG CAGTCATCGC AGTGCAGCTC TTCAAGGGGA AATTCTTCTA CTGCACCGAC
    GGCTCCATGG GCACGCAGAA AGAATGCCAG GGCTACTACA TCGACTATGG AAGAGACAGA
    AAGGAGGTGA AGAAGAGGGA ATGGAGGAGA CACGAGTTTC ACTATGATAA CGTCCTCTGG
    GCTCTTCTAA CGCTCTTCAC CGTGTCCACA GGAGAGGGAT GGCCACAAGT CCTTCAGCAC
    TCGGTGGACG TCACTGAAGA AGACCACGGC CCGAGCAGAG GAAACCGCAT GGAGATGTCC
    ATTTTCTACG TCATATACTT TGTGGTTTTC CCTTTCTTTT TCGTCAACAT TTTTGTAGCT
    CTCATCATTA TCACATTCCA GGAGCAGGGC GACAAAATGA TGGAGGAGTG CAACCTGGAG
    AAAAACGAGT ATTACTCAGC ACCGACGGCC TACGATATCG TCCTGAAGCA CCTCAACACG
    GCCTTCACCG TGCTGTTCTC TCTTGAGTGC ATCCTAAAGA TCATGGCCTT CGGGTTTATG
    AACTATTTCC GGGACACCTG GAACATCTTT GATTTCATCA CTGTGCTGGG CAGTATTACC
    GAAATTGTAG TAGACTTACA GTCAGTAAAC ACCATTAATA TGAGTTTCCT GAAGCTTTTC
    CGGGCAGCAA GACTGATCAA GCTATTGAGA CAAGGCTACA CGATACGCAT TCTGCTCTGG
    ACGTTTGTTC AGTCTTTCAA GGCTCTTCCA TATGTTTGTC TTCTTATTGC CATGCTTTTC
    TTCATTTATG CCATTATCGG CATGCAGGTG TTTGGAAATA TTAAACTGAA TGATGAAAGC
    CATATTAATC AGCACAACAA CTTCAAGACC TTCTTTGGTG CCCTGATGCT GCTGTTCAGA
    AGCGCCACAG GAGAGTCGTG GCAGGAGATC ATGCTGTCGT GTCTGGAGGG TAAAGAGTGT
    GAGCCGGACC CTTCAATAAC ACCACCTTTC ACCTCTCCAG ACCACGAGGG CGGCTGCGGC
    ACCGACTTCG CCTATTTCTA TTTCGTTTCC TTCATCTTCT TCAGCTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTATT TGACACGAGA TTCGTCAATT
    CTTGGACCTC ACCATCTAGA TGAGTTTGTG CGCATCTGGG GGGAATATGA CCGCGCAGCA
    TGTGGCCGGA TTCACTATAA TGCCATGTAT GAGATGCTGA CCCACATGTC TCCACCTCTT
    GGGTTGGGAA AGAAATGTCC TGCAAAAATT GCCTACAAGA GACTGGTGCT GATGAACATG
    CCAGTCGACG AGGACATGAC GGTTCACTTC ACATCCACAC TGATGTCTCT GATCCGCACC
    GCACTGGAGA TCAAGATCGC TAGAGGGGGT GAGGATCGGC TACAGCTGGA CATGGAGTTA
    CAGAAAGAGA TCAGTTTCAT CTGGCCACAT CTGTCCCAGA AGACTCTGGA CCTGCTGGTG
    CCGATAAACA AAGACACTGA CATGACCGTT GGAAAGATCT ATGCTTCGAG GATGATCATG
    GATTTCTTTA AACAGAGCAA AGCCAAGAAG CTGAGGCTGC AAGTGGAGGC GCAGAGAGCT
    GCTGCACTCC CTCTGATATC TCACAGTGCT GGATCTGCCC TGACCAGCGG AGGGTTTGTG
    GCTTTAAGCC CAATTTCACC ACAGGAGCTG TTATTGCAGC CCATAACCCA GCGTACAGAC
    AGCAGTGAAG ACTACGACGC CTCACAGGTT GATGAATCAG GGTTGAATGG CGTGTGGGGG
    GAAGACCGCC CACCTGAACA TGCATTCAGA GCCAGACACA AGAGCTACAA AGCTGCAGTG
    TCCCACTCAG AGCAGACACA GTACATGGAA AAGGTGCGAG GACGACCAAA GGAACAACGG
    CTCTTACTGT CCCCGAAAAA CTCCAACTCA AAGTCTCGTT CCCGCTCTCC CAGCGAGGAG
    CGCGTACATG GGGTCACAAG ACAGGGAAAC AGCTCCGAAA GCCCCGTTCC TTCCACCTCA
    GAGTCCAGCA CCCCGAGTGG AAAACGCAGA TTATCCCAAA CCTCCACCTG CTCTCGGCCT
    CACATCTCCT ACTCGCCTCT AGTGTCCCAC ACGCAGTCTT CATCGCAGCC CACTAATAAT
    AATGAGGAGG ATGAGGAGGA TGATCCAGCG GTTCAGCAGA TGTTAGGCTG CTGCAGTACT
    GCTTTATGGG ATGACAGCGC TGAAGACCAG GATGCGAGAG CGAGACAACA AGAAAAATCC
    CATTTCGAAG TGGATGAATT TTTGGGTTCG TCCGTCCGCC GTTATCCATC TGAATCATTC
    CTCGCTCTCC AAGAGGAAGA GCACAGCCCG GATTCTGCCA CAGCAATGGA AACTCTCACT
    TTTGAGGCGG CGGTGGCCAC CAGCTTAGGC CGCGCAAATA CGGTCAGCGC CTCAATGCGC
    ACTAGATCAC GAATAGGCTG GCAGGTGCCC AATGGACACT TCCGGAAGAG GTTGGCGCAG
    AGTGTTTCTC TTGCTGGGGG CGAAACTCTG AGCGATGTAG AGGACGACAA ATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206703.1
    CDS8..6124
    Translation

    Target ORF information:

    RefSeq Version XM_017351214.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, R type, alpha 1E subunit b (cacna1eb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351214.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATCCTGG CCACCATCAT AGCCAACTGC ATCGTTCTGT CCCTGGAGCA GCATCTTCCT 
    GGAGAGGACA AGACACCCAT GTCCAAAAGA CTGGAGAAGA CGGAGCCTTA CTTCATTGGC
    ATCTTCTGTT TCGAGGCTGG CATCAAGCTG GTGGCGCTCG GCTTTGTTTT CCATAAGGGC
    TCCTATCTGA GAAACGGCTG GAACGTGATG GACTTCATCG TTGTGCTCAG CGGGATTCTG
    GCCACCGCTG GATCTCATAT GAACATTCCT GTTGATCTGA GAACGCTGAG AGCTGTGCGC
    GTGCTGAGAC CCCTGAAACT GGTCTCCGGT ATCCCCAGTT TACAGATCGT GCTGAAGTCC
    ATCATAAAAG CGATGGTGCC GCTGCTGCAG ATCGGACTCC TGCTGTTCTT CGCCATCCTG
    ATGTTCGCCA TCATCGGTCT GGAGTTTTAC AGTGGGAAAC TACACCACAC CTGCCTGGCC
    GACCTGGATA TACTCGATAA TGAGACGGTG GACTCGTCGG AGGTGGATTT TGCTTGTGGT
    GTGAGGAAAT GTCCTGATAA ATACACCTGC AGCGGCTCTT GGATTGGACC CAATGACGGC
    ATCACGCAGT TTGATAACAT CCTGTTCGCA GTGCTAACAG TGTTTCAGTG CATCACTATG
    GAGGGCTGGA CGGCGGTGCT GTATAATACT AATGATGCTC TGGGACCCAC GTGGAATTGG
    ATATATTTCA TTCCTCTCAT CATTATCGGC TCATTCTTTG TGCTCAACCT TGTTTTGGGT
    GTCCTTTCTG GGGAGTTTGC TAAAGAGAGA GAGCGGGTGG AGAACAGACG TGCTTTCATG
    AAACTCAGAC GACAACAGCA GGTGGAGAGA GAACTGAACG GCTACCGGGC ATGGATAGAC
    AGAGCAGAGG AGGTCATGCT TCAAGAAGAA AACAAGAATT CTGGGAGGTC TGCATTAGAT
    GTATTGAAAC GAGCCACAAG CAAGAAAAAC GCAAGACGTC GAGGGCCAGG AGAGGAGAAA
    TATTCTGAAA TCTCCACAGT TGCAGGTGGA TCTCGTCCGA GGGTTGGCAT GCGGACGCCG
    AGGCGAGGTC CTGCTGCGTA TCTGAGGCGT AAAGAGCGAA TGTTACGCTT CTCCATTCGC
    CGCATGGTGA AAACCGACAG CTTCTACTGG ATAGTGCTCA GTCTGGTGGC GCTCAACACC
    ATCTCTGTGT CCATCGTTCA CCACAACCAG CCCGAATGGC TCACCGTCAT TCAGTATTAC
    ACAGAGTTTG TGTTTCTGGG GCTTTTTCTT GCTGAGATGT TTCTGAAAAT GTACGGCCTT
    GGCTTCCGTC TGTACTTCCA TTCGTCCTTC AACTGCTTTG ACTGTGGGGT GATTGTCGGC
    AGTATTTTTG AAGTGGTTTG GGGATTCTTC AGGCCTGGGG TGTCTTTTGG CATCAGTGTC
    CTCCGAGCTT TACGTCTGCT GCGGATCTTT AAAATTACCA AGTATTGGTC GTCCTTGAGG
    AATTTGGTGG TGTCCCTTAT GAGCTCCATG AAGTCCATCA TCAGTTTGCT CTTCCTCCTA
    TTCCTGTTTA TCGTCGTCTT CGCTCTTCTC GGCATGCAGC TGTTTGGCGG CAGGTTCATC
    TTTGAGGATT ACACTCCTAC TAATTTTGAC ACATTTCCAG CATCCATCAT GACGGTGTTT
    CAGATTCTCA CCGGAGAGGA CTGGAATGAA GTGATGTACA ACGGGATTCG TTCTCAAGGA
    GGAGTGCATT ATGGGATGTG GTCTTCCATA TACTTCATCG TTTTAACTTT GTTTGGGAAC
    TACACACTGC TCAATGTATT CTTGGCCATT GCTGTGGATA ATCTAGCCAA TGCACAGGAA
    CTAACTAAGG AAGAAGAGGA GGAGGAGGAG GAGTTCTTCA ACCAGCGCTA TAAAAGCAGA
    GAATTCGGCC TCTCGGAAAG GAGACGGAGA CCGTACCTGT ACAGAAAGCG TGCCATCCAC
    CGAGGGCGAC CTTCACCTGC AGAAGCGGAG GAGGAAGCTG CAGGGAAACA AGCAGCGGAA
    GAACAGCCAG TCAATGCTTT TGCAGGCCGT CGAGAGAGAC GCAAAAAAAT TAACATGTCC
    GTCTGGGAGC AAAGGGCCAA CCAGCTGCGG AAACGTCGTC AGATGGCCAG CCGTGAGGTA
    CTGTTTGGTG GTCCCACAGA GGACCAGGAC ACTCCAGGGA GCGATCATCA CCAGCAGCAT
    AGCTTGTCCA CTGAAACAAG CCCTTTACAC TTGCCAGAAT CTCCAATGTC TGTGGGGATG
    CCTTTGCCAG AGCCGCCTAT GTCTATTACC ATTCCTCTGC CTGAGCCTCC AGAGTCTGAG
    CCGTTGTCGG GATCAGGGCT AGAGGAGAGG CCGAGCGCTA ATAACCACCA CGCTAATACC
    GAAAGGAGAC ATCGAGTGGC GAGGAAGTTT CGAGCTGCTG CTGCTGCAGG GGTGGCGGAG
    GGTGGGCGAC ACAAACGCCA CAGACATCGG GAGTCCAGAA CAGAGGCCAG AAATGCAGAG
    AATCACCAGC AGTTTGAGAG GCAGGCTGAA GACGGTGAAG GTAAAACAGA GGAAGCTCAA
    TCTAAGCACA TGATGAACAT GTGTGACCCG GAAAGGCAAA TCGAGAATCA AGAGGAGTGC
    CAAAGGGAGT TTGTGTGTGA TCTGACAGGG CTCTCCCAAG CCTCCGGCCA TCAGGAGGAA
    GATCAAGCCG AAAGCCAAGA GGCTCAACTT CTGAGGCACA AAGAAGACTT CTCTACAGAA
    TCTAAATACA TGACAGAAGT CTCGGACATT GAGAACAACG GCAGCTATCT CAACCAGGAT
    GAGTGTATGA CCGAAACCAG CCTAAAACGA GAGTCCTCAT CCCAGCCTTT GTTGGAAATG
    GCACCAATGA CTGTCAGTTT CAAGGACGTG GAGGCGTCTC TTTCAGACAA AGCTGACGAT
    GATGATGATG ATGATATGGA TGATGATGAT GATTACGAAG ACGATGAGAA TGGAGGTGAA
    AAGTCGACAG CCTCCAAACC TGAAAGGCCC GACAGCATGT TCATTTTCAA GAGCAAAAAC
    CCCATCAGGA GGATCTGCCA TTATGTGGTG ACCCTGCGGT ATTTCGAGAT GACTATTCTC
    CTGGTGATCG TGGCCAGCAG TATCGCTCTG GCAGCTGAAG ACCCTGTGTG CACCAGTTCA
    GAGAGAAATA AGGTTCTGCG TTACTTTGAT TATGTTTTCA CTGGAGTTTT CACATTTGAG
    ATGATCATAA AGATGATTGA CCAGGGCCTC ATTCTTCACG ATGGGTCGTA TTTTCGGGAC
    ATGTGGAACA TTCTGGATTT TATTGTGGTG GTGGGAGCTC TGATTGCGTT TGCATTGACG
    AATTTATTGG GAAACAACAA AGGCCGAGAC ATTAAGACCA TCAAGTCCTT GCGGGTTCTG
    CGGGTTCTCC GTCCCCTCAA GACCATCAAA AGACTCCCAA AGCTGAAGGC AGTGTTTGAT
    TGCGTGGTCA CGTCTCTGAA GAACGTCTTC AACATCCTGA TCGTCTACAA GCTCTTCATG
    TTCATCTTCG CAGTCATCGC AGTGCAGCTC TTCAAGGGGA AATTCTTCTA CTGCACCGAC
    GGCTCCATGG GCACGCAGAA AGAATGCCAG GGCTACTACA TCGACTATGG AAGAGACAGA
    AAGGAGGTGA AGAAGAGGGA ATGGAGGAGA CACGAGTTTC ACTATGATAA CGTCCTCTGG
    GCTCTTCTAA CGCTCTTCAC CGTGTCCACA GGAGAGGGAT GGCCACAAGT CCTTCAGCAC
    TCGGTGGACG TCACTGAAGA AGACCACGGC CCGAGCAGAG GAAACCGCAT GGAGATGTCC
    ATTTTCTACG TCATATACTT TGTGGTTTTC CCTTTCTTTT TCGTCAACAT TTTTGTAGCT
    CTCATCATTA TCACATTCCA GGAGCAGGGC GACAAAATGA TGGAGGAGTG CAACCTGGAG
    AAAAACGAGT ATTACTCAGC ACCGACGGCC TACGATATCG TCCTGAAGCA CCTCAACACG
    GCCTTCACCG TGCTGTTCTC TCTTGAGTGC ATCCTAAAGA TCATGGCCTT CGGGTTTATG
    AACTATTTCC GGGACACCTG GAACATCTTT GATTTCATCA CTGTGCTGGG CAGTATTACC
    GAAATTGTAG TAGACTTACA GTCAGTAAAC ACCATTAATA TGAGTTTCCT GAAGCTTTTC
    CGGGCAGCAA GACTGATCAA GCTATTGAGA CAAGGCTACA CGATACGCAT TCTGCTCTGG
    ACGTTTGTTC AGTCTTTCAA GGCTCTTCCA TATGTTTGTC TTCTTATTGC CATGCTTTTC
    TTCATTTATG CCATTATCGG CATGCAGGTG TTTGGAAATA TTAAACTGAA TGATGAAAGC
    CATATTAATC AGCACAACAA CTTCAAGACC TTCTTTGGTG CCCTGATGCT GCTGTTCAGA
    AGCGCCACAG GAGAGTCGTG GCAGGAGATC ATGCTGTCGT GTCTGGAGGG TAAAGAGTGT
    GAGCCGGACC CTTCAATAAC ACCACCTTTC ACCTCTCCAG ACCACGAGGG CGGCTGCGGC
    ACCGACTTCG CCTATTTCTA TTTCGTTTCC TTCATCTTCT TCAGCTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTATT TGACACGAGA TTCGTCAATT
    CTTGGACCTC ACCATCTAGA TGAGTTTGTG CGCATCTGGG GGGAATATGA CCGCGCAGCA
    TGTGGCCGGA TTCACTATAA TGCCATGTAT GAGATGCTGA CCCACATGTC TCCACCTCTT
    GGGTTGGGAA AGAAATGTCC TGCAAAAATT GCCTACAAGA GACTGGTGCT GATGAACATG
    CCAGTCGACG AGGACATGAC GGTTCACTTC ACATCCACAC TGATGTCTCT GATCCGCACC
    GCACTGGAGA TCAAGATCGC TAGAGGGGGT GAGGATCGGC TACAGCTGGA CATGGAGTTA
    CAGAAAGAGA TCAGTTTCAT CTGGCCACAT CTGTCCCAGA AGACTCTGGA CCTGCTGGTG
    CCGATAAACA AAGACACTGA CATGACCGTT GGAAAGATCT ATGCTTCGAG GATGATCATG
    GATTTCTTTA AACAGAGCAA AGCCAAGAAG CTGAGGCTGC AAGTGGAGGC GCAGAGAGCT
    GCTGCACTCC CTCTGATATC TCACAGTGCT GGATCTGCCC TGACCAGCGG AGGGTTTGTG
    GCTTTAAGCC CAATTTCACC ACAGGAGCTG TTATTGCAGC CCATAACCCA GCGTACAGAC
    AGCAGTGAAG ACTACGACGC CTCACAGGTT GATGAATCAG GGTTGAATGG CGTGTGGGGG
    GAAGACCGCC CACCTGAACA TGCATTCAGA GCCAGACACA AGAGCTACAA AGCTGCAGTG
    TCCCACTCAG AGCAGACACA GTACATGGAA AAGGTGCGAG GACGACCAAA GGAACAACGG
    CTCTTACTGT CCCCGAAAAA CTCCAACTCA AAGTCTCGTT CCCGCTCTCC CAGCGAGGAG
    CGCGTACATG GGGTCACAAG ACAGGGAAAC AGCTCCGAAA GCCCCGTTCC TTCCACCTCA
    GAGTCCAGCA CCCCGAGTGG AAAACGCAGA TTATCCCAAA CCTCCACCTG CTCTCGGCCT
    CACATCTCCT ACTCGCCTCT AGTGTCCCAC ACGCAGTCTT CATCGCAGCC CACTAATAAT
    AATGAGGAGG ATGAGGAGGA TGATCCAGCG GTTCAGCAGA TGTTAGGCTG CTGCAGTACT
    GCTTTATGGG ATGACAGCGC TGAAGACCAG GATGCGAGAG CGAGACAACA AGAAAAATCC
    CATTTCGAAG TGGATGAATT TTTGGGTTCG TCCGTCCGCC GTTATCCATC TGAATCATTC
    CTCGCTCTCC AAGAGGAAGA GCACAGCCCG GATTCTGCCA CAGCAATGGA AACTCTCACT
    TTTGAGGCGG CGGTGGCCAC CAGCTTAGGC CGCGCAAATA CGGTCAGCGC CTCAATGCGC
    ACTAGATCAC GAATAGGCTG GCAGGTGCCC AATGGACACT TCCGGAAGAG GTTGGCGCAG
    AGTGTTTCTC TTGCTGGGGG CGAAACTCTG AGCGATGTAG AGGACGACAA ATACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.