ralgapa2 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following ralgapa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ralgapa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149747 XM_021467463.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa149748 XM_021467464.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa149749 XM_021467465.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55740 XM_017351840.1
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa22782 XM_005158905.4
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa22783 XM_005158906.4
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa22784 XM_005158907.4
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa45361 XM_009293239.2
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa22779 XM_001920473.6
Latest version!
Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa22784 XM_005158907.3 Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa22779 XM_001920473.5 Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa22782 XM_005158905.3 Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa22783 XM_005158906.3 Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149747
    Clone ID Related Accession (Same CDS sequence) XM_021467463.1
    Accession Version XM_021467463.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG CAGGCAGGCG TTGGCCAGGG ATGCTTTGGG ACGTGGATTA CCTGTGGGCC
    AAGCTGGACT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323138.1
    CDS132..6086
    Translation

    Target ORF information:

    RefSeq Version XM_021467463.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG CAGGCAGGCG TTGGCCAGGG ATGCTTTGGG ACGTGGATTA CCTGTGGGCC
    AAGCTGGACT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149748
    Clone ID Related Accession (Same CDS sequence) XM_021467464.1
    Accession Version XM_021467464.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GCAGGCGTTG GCCAGGGATG CTTTGGGACG TGGATTACCT GTGGGCCAAG
    CTGGACTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323139.1
    CDS132..6083
    Translation

    Target ORF information:

    RefSeq Version XM_021467464.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GCAGGCGTTG GCCAGGGATG CTTTGGGACG TGGATTACCT GTGGGCCAAG
    CTGGACTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149749
    Clone ID Related Accession (Same CDS sequence) XM_021467465.1
    Accession Version XM_021467465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG CAGGCAGGCG TTGGCCAGGG ATGCTTTGGG ACGTGGATTA CCTGTGGGCC
    AAGCTGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323140.1
    CDS132..6083
    Translation

    Target ORF information:

    RefSeq Version XM_021467465.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG CAGGCAGGCG TTGGCCAGGG ATGCTTTGGG ACGTGGATTA CCTGTGGGCC
    AAGCTGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55740
    Clone ID Related Accession (Same CDS sequence) XM_017351840.1
    Accession Version XM_017351840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GATATACACA CATGCTGTTT GAAAGCTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207329.1
    CDS115..6036
    Translation

    Target ORF information:

    RefSeq Version XM_017351840.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GATATACACA CATGCTGTTT GAAAGCTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22782
    Clone ID Related Accession (Same CDS sequence) XM_005158905.3 , XM_005158905.4
    Accession Version XM_005158905.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGA CAGCAGTGAG TTTCTGGCTG ATGACATCAG CATTATCGCC
    GGTGGCAGTT TGACTGGTTG GCATGCGGAC TCGGCCTTCG TTCTCTGGAG GAGGATTCTG
    GGTATTCTTG GTGATGTGAA CAGCATCCGC TGTGCCCGCA TCCACGCCAA GGTCTTCAGT
    TACCTCTATG AGCTCTGGCA CAAGCTGGCC AAGATCAGAG ATAATCTTGG GATCAGAGTG
    GATTCAGACA CAGCCATGGT CAAACCCTTG TTCATCCCTC CTCTGCGTAT GTTGGCCTCC
    TGGCTCTTTA AGGCGACCAT GCTGCCTTCA GAATTTAAAG CTGGGAAACT ACAGGCGTAT
    AAGCTGATCT GTGAGATGAT GACCAAACAT CAAGACGTGC TGCCCAACAG TGACTTCCTG
    GTGCATCTAT ACCATGTCAT GCACAAAGGG TTCACAAGCG ACGATCAGGA TGTGTTGAAC
    ACAATGATCA GGTGGTGCTC TCCTCGCTTC TTCTTTCTGG GTCTGCCGGG CTTCACCATG
    CTAGTGGGAG ACTTCATCAC CGCGGCTGCT CGCATCCTCA ACACAGACTC CTTTGAGGCT
    CCACGAGTCG AGGCTCAGGC TGTTCTTGGT TCGCTGGTGT GTTTTCCGAA CTTGTATCAG
    CAGATTCCCT CTCTTCAGTC TGTTCCTGGA TCTGAGGATA TCCTCGTTGG CAAAGAGGAC
    ATGAAGGACT ATCTTGTGAA CATTCTTTTG AAGACTGCAC GCAGTGAGAC ATGTGAAGGA
    GCCAGGTGTG TGGCGATCTG CGGTCTGGGT TTGTGGGTGT GTGAGGAGCT CAGGCAAACA
    GAAACCCATC ATCAGGTCAA AGATGCCATC AACGTCCTCG GTGTCACGCT CAAGTTTGGG
    AATAAAGTGG TTGCCAACGT GGCGTGCGAC ATCTTTCAGC TACTGATTTC TCACTGGCAG
    CACTTACAAA GACTAGAACC TTCTCTTCCC AAAAGAATCA TAGAGATTTT TGTAGCAACC
    GTAGCCTTTC TTCTGCCCAG CGCTGAACAC TCAACTGTGG AGGCAGACAA GAAGTTAATG
    GTGTCTCTAC TGCTGTGCCT GCTGGACTGG TGCATGTCTG TACCTCTCTC CATCCTGCTG
    GAGCCCATCA CTATGCCTGT GCTGGACGAC CCCACCTCCC AAAAGGCCCC GCTGCTCGAC
    TACATTTACA GGGTGCTTCA CACTTGTGTA TCAGGGTCTA ACCTGCACAC TCAGCAGAGT
    CACTACCTCC TCAGCCTGGC TGATCTATCA ACAGACTACG AACCCTTCCT TATGCTGGGC
    AATGTCAAGA GCTTTGAACC ACCGGCCATA CACAACGCCA TGGGAGATTT TGGAAATCTC
    CTCACTGTGG CTGAAGAAAA GAAAAGGAGA AGCATGGAAC TGATACCCCT AACTGCTCGC
    ATGGTAATGA CACACCTGGT CAATCATTTG GGCCACCATC CTCTGAGTGG CGGCCCGGCC
    CTCTTGAACA GCCTGATCAG CGAGAACCAC GACAACCCCT ATGTAGAGTC TTCAGAGCTC
    TCATCTGAGG TCTTCAAAAG CCCCAATCTG CAGCTCTTCG TCTTTAATGA CAGCACGCTC
    ATTTCATACC TGCAGATCCC CTCTGATAAT TCCAACACTG CTCCGCTAAG TGCCAACCCC
    AGGGATCATT CCTCTGAGGT CCGCATCATC ATTCGGGACA TCTCAGGCAA ATACTCATGG
    GACAGTGGCG TTCTCTTCCG CACTTTAGAT GGCACCGGTG TGACTTCCAG CGACAGCAGA
    GAACAGTCTT CTCATGAAGC TGCTCGATTA GAAAAGCGGA GCTCTGTCCG CTTCAGTAAA
    TGTTCATCAG AGCTGGACGT GGAAGATGGC GTTGATGTGC TGGACCAGCT CCTGGAGGAC
    CTCGGCCACA GCAGTCCCGA GTGTCTGCCG GAACCCCAGC TCAGACTGAC CCAGCCACCA
    TCTCCACCCA TGGGCATGAA CCTGGAGATA GAAGGGCTCA TCATGGAGGC CGTCCACCGA
    CAGTCAAAAC AGGAGGAAGA GGAGTTGATG CGAAGGCAAA TCGAAGACCC CAGTGTCAGA
    GCAGAGAGGC AGAAAGAGCC CACACATCAG GAGCCCAAAT CCCCTTTCTA CCTATGCAGA
    CTCCTCCTCA ATGACCTCGG CATGAACTCA TGGGATCGCA GAAAGAGCTT TCATCTCCTG
    AAGAAGAACT CCAAGCTTTT AAGAGAGCTG AAGAATTTGG ACTCGAGGCA GTGTCGAGAG
    ACACACAAGA TAGCAGTCTT CTATATTGGA GAAGGACAAG AGGACAAATA TTCCATTCTG
    TCCAATACAC AGGGCAGTCA GGTTTATGAG GACTTTGTCT CTGGACTGGG ATGGGAGGTG
    AATCTGGCCA CTCACTGTGG GTTTATGGGT GGTTTGCAGA GAAATGGCAG CACAGGAAGC
    ACAGCTCCAT ATTACGCTAC CTCCAATGTG GAGGTCATCT TCCACGTGTC CACTCGGATG
    CCTTCTGACT CTGATGACTC CATCACTAAA AAGCTTCGTC ACTTGGGAAA CGACGAAGTG
    CACATCGTGT GGTCGGAGCA CACGCGGGAT TACAGGCGAG GAATCATCCC CACCGACTTT
    GGAGATGTCC TCGTCATCAT CTACCCCATG AAAAACCACA TGTTCTTTGT TCAGATCATG
    AAAAAACCCC AGGTTCCTTT TTTTGGGCCT CTGTTTGATG GGGCGATAGT GACTGGAGAG
    TTGTTGCCGA GTTTGGTGCG AGCCACGTGC ATCAATGCCA GCAGGGCCGT GAAGTCTCGT
    CTGCCACTCT ACCAGAGTTT TTATGAAGAA AGAGCGCTGT ATCTGGAGGC CATCATTCAG
    AACCACAAGG AGAACATGAC CTTCGAGGAC TTCGCTGCTC AGGTCTTCTC TCCATCTCCC
    AGCTACTGGC CTTCTGGAGG TGCAGGATCC CTCAGCTGCA GCACGAGCGC TGACCTCAGT
    GTTTCTGTGA TGACCAATGA CTCTGGTGAC CAGGTTTCAC CCACAATACC ACGCGCTACC
    AAGAACCGGG TTTCTGGAAA ACTGCGGCGC TCAGCTAGTG CCATCAGCAA ATCCTCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158962.1
    CDS115..6057
    Translation

    Target ORF information:

    RefSeq Version XM_005158905.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158905.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGA CAGCAGTGAG TTTCTGGCTG ATGACATCAG CATTATCGCC
    GGTGGCAGTT TGACTGGTTG GCATGCGGAC TCGGCCTTCG TTCTCTGGAG GAGGATTCTG
    GGTATTCTTG GTGATGTGAA CAGCATCCGC TGTGCCCGCA TCCACGCCAA GGTCTTCAGT
    TACCTCTATG AGCTCTGGCA CAAGCTGGCC AAGATCAGAG ATAATCTTGG GATCAGAGTG
    GATTCAGACA CAGCCATGGT CAAACCCTTG TTCATCCCTC CTCTGCGTAT GTTGGCCTCC
    TGGCTCTTTA AGGCGACCAT GCTGCCTTCA GAATTTAAAG CTGGGAAACT ACAGGCGTAT
    AAGCTGATCT GTGAGATGAT GACCAAACAT CAAGACGTGC TGCCCAACAG TGACTTCCTG
    GTGCATCTAT ACCATGTCAT GCACAAAGGG TTCACAAGCG ACGATCAGGA TGTGTTGAAC
    ACAATGATCA GGTGGTGCTC TCCTCGCTTC TTCTTTCTGG GTCTGCCGGG CTTCACCATG
    CTAGTGGGAG ACTTCATCAC CGCGGCTGCT CGCATCCTCA ACACAGACTC CTTTGAGGCT
    CCACGAGTCG AGGCTCAGGC TGTTCTTGGT TCGCTGGTGT GTTTTCCGAA CTTGTATCAG
    CAGATTCCCT CTCTTCAGTC TGTTCCTGGA TCTGAGGATA TCCTCGTTGG CAAAGAGGAC
    ATGAAGGACT ATCTTGTGAA CATTCTTTTG AAGACTGCAC GCAGTGAGAC ATGTGAAGGA
    GCCAGGTGTG TGGCGATCTG CGGTCTGGGT TTGTGGGTGT GTGAGGAGCT CAGGCAAACA
    GAAACCCATC ATCAGGTCAA AGATGCCATC AACGTCCTCG GTGTCACGCT CAAGTTTGGG
    AATAAAGTGG TTGCCAACGT GGCGTGCGAC ATCTTTCAGC TACTGATTTC TCACTGGCAG
    CACTTACAAA GACTAGAACC TTCTCTTCCC AAAAGAATCA TAGAGATTTT TGTAGCAACC
    GTAGCCTTTC TTCTGCCCAG CGCTGAACAC TCAACTGTGG AGGCAGACAA GAAGTTAATG
    GTGTCTCTAC TGCTGTGCCT GCTGGACTGG TGCATGTCTG TACCTCTCTC CATCCTGCTG
    GAGCCCATCA CTATGCCTGT GCTGGACGAC CCCACCTCCC AAAAGGCCCC GCTGCTCGAC
    TACATTTACA GGGTGCTTCA CACTTGTGTA TCAGGGTCTA ACCTGCACAC TCAGCAGAGT
    CACTACCTCC TCAGCCTGGC TGATCTATCA ACAGACTACG AACCCTTCCT TATGCTGGGC
    AATGTCAAGA GCTTTGAACC ACCGGCCATA CACAACGCCA TGGGAGATTT TGGAAATCTC
    CTCACTGTGG CTGAAGAAAA GAAAAGGAGA AGCATGGAAC TGATACCCCT AACTGCTCGC
    ATGGTAATGA CACACCTGGT CAATCATTTG GGCCACCATC CTCTGAGTGG CGGCCCGGCC
    CTCTTGAACA GCCTGATCAG CGAGAACCAC GACAACCCCT ATGTAGAGTC TTCAGAGCTC
    TCATCTGAGG TCTTCAAAAG CCCCAATCTG CAGCTCTTCG TCTTTAATGA CAGCACGCTC
    ATTTCATACC TGCAGATCCC CTCTGATAAT TCCAACACTG CTCCGCTAAG TGCCAACCCC
    AGGGATCATT CCTCTGAGGT CCGCATCATC ATTCGGGACA TCTCAGGCAA ATACTCATGG
    GACAGTGGCG TTCTCTTCCG CACTTTAGAT GGCACCGGTG TGACTTCCAG CGACAGCAGA
    GAACAGTCTT CTCATGAAGC TGCTCGATTA GAAAAGCGGA GCTCTGTCCG CTTCAGTAAA
    TGTTCATCAG AGCTGGACGT GGAAGATGGC GTTGATGTGC TGGACCAGCT CCTGGAGGAC
    CTCGGCCACA GCAGTCCCGA GTGTCTGCCG GAACCCCAGC TCAGACTGAC CCAGCCACCA
    TCTCCACCCA TGGGCATGAA CCTGGAGATA GAAGGGCTCA TCATGGAGGC CGTCCACCGA
    CAGTCAAAAC AGGAGGAAGA GGAGTTGATG CGAAGGCAAA TCGAAGACCC CAGTGTCAGA
    GCAGAGAGGC AGAAAGAGCC CACACATCAG GAGCCCAAAT CCCCTTTCTA CCTATGCAGA
    CTCCTCCTCA ATGACCTCGG CATGAACTCA TGGGATCGCA GAAAGAGCTT TCATCTCCTG
    AAGAAGAACT CCAAGCTTTT AAGAGAGCTG AAGAATTTGG ACTCGAGGCA GTGTCGAGAG
    ACACACAAGA TAGCAGTCTT CTATATTGGA GAAGGACAAG AGGACAAATA TTCCATTCTG
    TCCAATACAC AGGGCAGTCA GGTTTATGAG GACTTTGTCT CTGGACTGGG ATGGGAGGTG
    AATCTGGCCA CTCACTGTGG GTTTATGGGT GGTTTGCAGA GAAATGGCAG CACAGGAAGC
    ACAGCTCCAT ATTACGCTAC CTCCAATGTG GAGGTCATCT TCCACGTGTC CACTCGGATG
    CCTTCTGACT CTGATGACTC CATCACTAAA AAGCTTCGTC ACTTGGGAAA CGACGAAGTG
    CACATCGTGT GGTCGGAGCA CACGCGGGAT TACAGGCGAG GAATCATCCC CACCGACTTT
    GGAGATGTCC TCGTCATCAT CTACCCCATG AAAAACCACA TGTTCTTTGT TCAGATCATG
    AAAAAACCCC AGGTTCCTTT TTTTGGGCCT CTGTTTGATG GGGCGATAGT GACTGGAGAG
    TTGTTGCCGA GTTTGGTGCG AGCCACGTGC ATCAATGCCA GCAGGGCCGT GAAGTCTCGT
    CTGCCACTCT ACCAGAGTTT TTATGAAGAA AGAGCGCTGT ATCTGGAGGC CATCATTCAG
    AACCACAAGG AGAACATGAC CTTCGAGGAC TTCGCTGCTC AGGTCTTCTC TCCATCTCCC
    AGCTACTGGC CTTCTGGAGG TGCAGGATCC CTCAGCTGCA GCACGAGCGC TGACCTCAGT
    GTTTCTGTGA TGACCAATGA CTCTGGTGAC CAGGTTTCAC CCACAATACC ACGCGCTACC
    AAGAACCGGG TTTCTGGAAA ACTGCGGCGC TCAGCTAGTG CCATCAGCAA ATCCTCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22782
    Clone ID Related Accession (Same CDS sequence) XM_005158905.3 , XM_005158905.4
    Accession Version XM_005158905.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGA CAGCAGTGAG TTTCTGGCTG ATGACATCAG CATTATCGCC
    GGTGGCAGTT TGACTGGTTG GCATGCGGAC TCGGCCTTCG TTCTCTGGAG GAGGATTCTG
    GGTATTCTTG GTGATGTGAA CAGCATCCGC TGTGCCCGCA TCCACGCCAA GGTCTTCAGT
    TACCTCTATG AGCTCTGGCA CAAGCTGGCC AAGATCAGAG ATAATCTTGG GATCAGAGTG
    GATTCAGACA CAGCCATGGT CAAACCCTTG TTCATCCCTC CTCTGCGTAT GTTGGCCTCC
    TGGCTCTTTA AGGCGACCAT GCTGCCTTCA GAATTTAAAG CTGGGAAACT ACAGGCGTAT
    AAGCTGATCT GTGAGATGAT GACCAAACAT CAAGACGTGC TGCCCAACAG TGACTTCCTG
    GTGCATCTAT ACCATGTCAT GCACAAAGGG TTCACAAGCG ACGATCAGGA TGTGTTGAAC
    ACAATGATCA GGTGGTGCTC TCCTCGCTTC TTCTTTCTGG GTCTGCCGGG CTTCACCATG
    CTAGTGGGAG ACTTCATCAC CGCGGCTGCT CGCATCCTCA ACACAGACTC CTTTGAGGCT
    CCACGAGTCG AGGCTCAGGC TGTTCTTGGT TCGCTGGTGT GTTTTCCGAA CTTGTATCAG
    CAGATTCCCT CTCTTCAGTC TGTTCCTGGA TCTGAGGATA TCCTCGTTGG CAAAGAGGAC
    ATGAAGGACT ATCTTGTGAA CATTCTTTTG AAGACTGCAC GCAGTGAGAC ATGTGAAGGA
    GCCAGGTGTG TGGCGATCTG CGGTCTGGGT TTGTGGGTGT GTGAGGAGCT CAGGCAAACA
    GAAACCCATC ATCAGGTCAA AGATGCCATC AACGTCCTCG GTGTCACGCT CAAGTTTGGG
    AATAAAGTGG TTGCCAACGT GGCGTGCGAC ATCTTTCAGC TACTGATTTC TCACTGGCAG
    CACTTACAAA GACTAGAACC TTCTCTTCCC AAAAGAATCA TAGAGATTTT TGTAGCAACC
    GTAGCCTTTC TTCTGCCCAG CGCTGAACAC TCAACTGTGG AGGCAGACAA GAAGTTAATG
    GTGTCTCTAC TGCTGTGCCT GCTGGACTGG TGCATGTCTG TACCTCTCTC CATCCTGCTG
    GAGCCCATCA CTATGCCTGT GCTGGACGAC CCCACCTCCC AAAAGGCCCC GCTGCTCGAC
    TACATTTACA GGGTGCTTCA CACTTGTGTA TCAGGGTCTA ACCTGCACAC TCAGCAGAGT
    CACTACCTCC TCAGCCTGGC TGATCTATCA ACAGACTACG AACCCTTCCT TATGCTGGGC
    AATGTCAAGA GCTTTGAACC ACCGGCCATA CACAACGCCA TGGGAGATTT TGGAAATCTC
    CTCACTGTGG CTGAAGAAAA GAAAAGGAGA AGCATGGAAC TGATACCCCT AACTGCTCGC
    ATGGTAATGA CACACCTGGT CAATCATTTG GGCCACCATC CTCTGAGTGG CGGCCCGGCC
    CTCTTGAACA GCCTGATCAG CGAGAACCAC GACAACCCCT ATGTAGAGTC TTCAGAGCTC
    TCATCTGAGG TCTTCAAAAG CCCCAATCTG CAGCTCTTCG TCTTTAATGA CAGCACGCTC
    ATTTCATACC TGCAGATCCC CTCTGATAAT TCCAACACTG CTCCGCTAAG TGCCAACCCC
    AGGGATCATT CCTCTGAGGT CCGCATCATC ATTCGGGACA TCTCAGGCAA ATACTCATGG
    GACAGTGGCG TTCTCTTCCG CACTTTAGAT GGCACCGGTG TGACTTCCAG CGACAGCAGA
    GAACAGTCTT CTCATGAAGC TGCTCGATTA GAAAAGCGGA GCTCTGTCCG CTTCAGTAAA
    TGTTCATCAG AGCTGGACGT GGAAGATGGC GTTGATGTGC TGGACCAGCT CCTGGAGGAC
    CTCGGCCACA GCAGTCCCGA GTGTCTGCCG GAACCCCAGC TCAGACTGAC CCAGCCACCA
    TCTCCACCCA TGGGCATGAA CCTGGAGATA GAAGGGCTCA TCATGGAGGC CGTCCACCGA
    CAGTCAAAAC AGGAGGAAGA GGAGTTGATG CGAAGGCAAA TCGAAGACCC CAGTGTCAGA
    GCAGAGAGGC AGAAAGAGCC CACACATCAG GAGCCCAAAT CCCCTTTCTA CCTATGCAGA
    CTCCTCCTCA ATGACCTCGG CATGAACTCA TGGGATCGCA GAAAGAGCTT TCATCTCCTG
    AAGAAGAACT CCAAGCTTTT AAGAGAGCTG AAGAATTTGG ACTCGAGGCA GTGTCGAGAG
    ACACACAAGA TAGCAGTCTT CTATATTGGA GAAGGACAAG AGGACAAATA TTCCATTCTG
    TCCAATACAC AGGGCAGTCA GGTTTATGAG GACTTTGTCT CTGGACTGGG ATGGGAGGTG
    AATCTGGCCA CTCACTGTGG GTTTATGGGT GGTTTGCAGA GAAATGGCAG CACAGGAAGC
    ACAGCTCCAT ATTACGCTAC CTCCAATGTG GAGGTCATCT TCCACGTGTC CACTCGGATG
    CCTTCTGACT CTGATGACTC CATCACTAAA AAGCTTCGTC ACTTGGGAAA CGACGAAGTG
    CACATCGTGT GGTCGGAGCA CACGCGGGAT TACAGGCGAG GAATCATCCC CACCGACTTT
    GGAGATGTCC TCGTCATCAT CTACCCCATG AAAAACCACA TGTTCTTTGT TCAGATCATG
    AAAAAACCCC AGGTTCCTTT TTTTGGGCCT CTGTTTGATG GGGCGATAGT GACTGGAGAG
    TTGTTGCCGA GTTTGGTGCG AGCCACGTGC ATCAATGCCA GCAGGGCCGT GAAGTCTCGT
    CTGCCACTCT ACCAGAGTTT TTATGAAGAA AGAGCGCTGT ATCTGGAGGC CATCATTCAG
    AACCACAAGG AGAACATGAC CTTCGAGGAC TTCGCTGCTC AGGTCTTCTC TCCATCTCCC
    AGCTACTGGC CTTCTGGAGG TGCAGGATCC CTCAGCTGCA GCACGAGCGC TGACCTCAGT
    GTTTCTGTGA TGACCAATGA CTCTGGTGAC CAGGTTTCAC CCACAATACC ACGCGCTACC
    AAGAACCGGG TTTCTGGAAA ACTGCGGCGC TCAGCTAGTG CCATCAGCAA ATCCTCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158962.1
    CDS131..6073
    Translation

    Target ORF information:

    RefSeq Version XM_005158905.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158905.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGA CAGCAGTGAG TTTCTGGCTG ATGACATCAG CATTATCGCC
    GGTGGCAGTT TGACTGGTTG GCATGCGGAC TCGGCCTTCG TTCTCTGGAG GAGGATTCTG
    GGTATTCTTG GTGATGTGAA CAGCATCCGC TGTGCCCGCA TCCACGCCAA GGTCTTCAGT
    TACCTCTATG AGCTCTGGCA CAAGCTGGCC AAGATCAGAG ATAATCTTGG GATCAGAGTG
    GATTCAGACA CAGCCATGGT CAAACCCTTG TTCATCCCTC CTCTGCGTAT GTTGGCCTCC
    TGGCTCTTTA AGGCGACCAT GCTGCCTTCA GAATTTAAAG CTGGGAAACT ACAGGCGTAT
    AAGCTGATCT GTGAGATGAT GACCAAACAT CAAGACGTGC TGCCCAACAG TGACTTCCTG
    GTGCATCTAT ACCATGTCAT GCACAAAGGG TTCACAAGCG ACGATCAGGA TGTGTTGAAC
    ACAATGATCA GGTGGTGCTC TCCTCGCTTC TTCTTTCTGG GTCTGCCGGG CTTCACCATG
    CTAGTGGGAG ACTTCATCAC CGCGGCTGCT CGCATCCTCA ACACAGACTC CTTTGAGGCT
    CCACGAGTCG AGGCTCAGGC TGTTCTTGGT TCGCTGGTGT GTTTTCCGAA CTTGTATCAG
    CAGATTCCCT CTCTTCAGTC TGTTCCTGGA TCTGAGGATA TCCTCGTTGG CAAAGAGGAC
    ATGAAGGACT ATCTTGTGAA CATTCTTTTG AAGACTGCAC GCAGTGAGAC ATGTGAAGGA
    GCCAGGTGTG TGGCGATCTG CGGTCTGGGT TTGTGGGTGT GTGAGGAGCT CAGGCAAACA
    GAAACCCATC ATCAGGTCAA AGATGCCATC AACGTCCTCG GTGTCACGCT CAAGTTTGGG
    AATAAAGTGG TTGCCAACGT GGCGTGCGAC ATCTTTCAGC TACTGATTTC TCACTGGCAG
    CACTTACAAA GACTAGAACC TTCTCTTCCC AAAAGAATCA TAGAGATTTT TGTAGCAACC
    GTAGCCTTTC TTCTGCCCAG CGCTGAACAC TCAACTGTGG AGGCAGACAA GAAGTTAATG
    GTGTCTCTAC TGCTGTGCCT GCTGGACTGG TGCATGTCTG TACCTCTCTC CATCCTGCTG
    GAGCCCATCA CTATGCCTGT GCTGGACGAC CCCACCTCCC AAAAGGCCCC GCTGCTCGAC
    TACATTTACA GGGTGCTTCA CACTTGTGTA TCAGGGTCTA ACCTGCACAC TCAGCAGAGT
    CACTACCTCC TCAGCCTGGC TGATCTATCA ACAGACTACG AACCCTTCCT TATGCTGGGC
    AATGTCAAGA GCTTTGAACC ACCGGCCATA CACAACGCCA TGGGAGATTT TGGAAATCTC
    CTCACTGTGG CTGAAGAAAA GAAAAGGAGA AGCATGGAAC TGATACCCCT AACTGCTCGC
    ATGGTAATGA CACACCTGGT CAATCATTTG GGCCACCATC CTCTGAGTGG CGGCCCGGCC
    CTCTTGAACA GCCTGATCAG CGAGAACCAC GACAACCCCT ATGTAGAGTC TTCAGAGCTC
    TCATCTGAGG TCTTCAAAAG CCCCAATCTG CAGCTCTTCG TCTTTAATGA CAGCACGCTC
    ATTTCATACC TGCAGATCCC CTCTGATAAT TCCAACACTG CTCCGCTAAG TGCCAACCCC
    AGGGATCATT CCTCTGAGGT CCGCATCATC ATTCGGGACA TCTCAGGCAA ATACTCATGG
    GACAGTGGCG TTCTCTTCCG CACTTTAGAT GGCACCGGTG TGACTTCCAG CGACAGCAGA
    GAACAGTCTT CTCATGAAGC TGCTCGATTA GAAAAGCGGA GCTCTGTCCG CTTCAGTAAA
    TGTTCATCAG AGCTGGACGT GGAAGATGGC GTTGATGTGC TGGACCAGCT CCTGGAGGAC
    CTCGGCCACA GCAGTCCCGA GTGTCTGCCG GAACCCCAGC TCAGACTGAC CCAGCCACCA
    TCTCCACCCA TGGGCATGAA CCTGGAGATA GAAGGGCTCA TCATGGAGGC CGTCCACCGA
    CAGTCAAAAC AGGAGGAAGA GGAGTTGATG CGAAGGCAAA TCGAAGACCC CAGTGTCAGA
    GCAGAGAGGC AGAAAGAGCC CACACATCAG GAGCCCAAAT CCCCTTTCTA CCTATGCAGA
    CTCCTCCTCA ATGACCTCGG CATGAACTCA TGGGATCGCA GAAAGAGCTT TCATCTCCTG
    AAGAAGAACT CCAAGCTTTT AAGAGAGCTG AAGAATTTGG ACTCGAGGCA GTGTCGAGAG
    ACACACAAGA TAGCAGTCTT CTATATTGGA GAAGGACAAG AGGACAAATA TTCCATTCTG
    TCCAATACAC AGGGCAGTCA GGTTTATGAG GACTTTGTCT CTGGACTGGG ATGGGAGGTG
    AATCTGGCCA CTCACTGTGG GTTTATGGGT GGTTTGCAGA GAAATGGCAG CACAGGAAGC
    ACAGCTCCAT ATTACGCTAC CTCCAATGTG GAGGTCATCT TCCACGTGTC CACTCGGATG
    CCTTCTGACT CTGATGACTC CATCACTAAA AAGCTTCGTC ACTTGGGAAA CGACGAAGTG
    CACATCGTGT GGTCGGAGCA CACGCGGGAT TACAGGCGAG GAATCATCCC CACCGACTTT
    GGAGATGTCC TCGTCATCAT CTACCCCATG AAAAACCACA TGTTCTTTGT TCAGATCATG
    AAAAAACCCC AGGTTCCTTT TTTTGGGCCT CTGTTTGATG GGGCGATAGT GACTGGAGAG
    TTGTTGCCGA GTTTGGTGCG AGCCACGTGC ATCAATGCCA GCAGGGCCGT GAAGTCTCGT
    CTGCCACTCT ACCAGAGTTT TTATGAAGAA AGAGCGCTGT ATCTGGAGGC CATCATTCAG
    AACCACAAGG AGAACATGAC CTTCGAGGAC TTCGCTGCTC AGGTCTTCTC TCCATCTCCC
    AGCTACTGGC CTTCTGGAGG TGCAGGATCC CTCAGCTGCA GCACGAGCGC TGACCTCAGT
    GTTTCTGTGA TGACCAATGA CTCTGGTGAC CAGGTTTCAC CCACAATACC ACGCGCTACC
    AAGAACCGGG TTTCTGGAAA ACTGCGGCGC TCAGCTAGTG CCATCAGCAA ATCCTCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22783
    Clone ID Related Accession (Same CDS sequence) XM_005158906.3 , XM_005158906.4
    Accession Version XM_005158906.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGATGTG
    GAAGAGTGTC AGCTGTCGGA GTGTGAGATG GGTGATGGTG ACAGTCCTCT GCCCCGCAGC
    AGCAGCACCT CTGACATCAC ACAGCAAATC AACGACACCT TTACAGGTCA GAGGAAAGAT
    GAGAGTATGG ATCTGGTCAG ATCATCTGTA TCGGAGAGCA GCTCTGCAGG GACGAGAGAA
    AGTGACGCTC CAGTGATTCT GATTGAGCGA AGCAACAGTC CAGCAGATCT GGATTATCCA
    GCTGAAGGAG CTCCAGCTTT CTTGAAGATG AAACTCAGAG AGAAAAGTGA GAGTGTGAGC
    AGTGAGACGT CCAATGGCTA TCTGAATGAG GCAGAAGTGG GTCTGCTGTC GTGGCACACG
    CTGGAGGAAG AGCCGGACTC TCCCTCGACT CAAGACGTGA GCATGAGCAT GAGTGTTACA
    GCAGACACGG TGAGCCAGAG GAGTCTCCTG CTCAGCCACA CTGAAGCACT GGCAGGGTCT
    CCTCACTTTC CCCCAGTGTC TCCGGCTCTT CTGGTGCCTC ATGGCTGCTG TGAGGGCCCT
    CAGCTCCTGG AGGATGCCAT GAACATGCCT CAGCATCTGG ACAGCAGTGA GTTTCTGGCT
    GATGACATCA GCATTATCGC CGGTGGCAGT TTGACTGGTT GGCATGCGGA CTCGGCCTTC
    GTTCTCTGGA GGAGGATTCT GGGTATTCTT GGTGATGTGA ACAGCATCCG CTGTGCCCGC
    ATCCACGCCA AGGTCTTCAG TTACCTCTAT GAGCTCTGGC ACAAGCTGGC CAAGATCAGA
    GATAATCTTG GGATCAGAGT GGATTCAGAC ACAGCCATGG TCAAACCCTT GTTCATCCCT
    CCTCTGCGTA TGTTGGCCTC CTGGCTCTTT AAGGCGACCA TGCTGCCTTC AGAATTTAAA
    GCTGGGAAAC TACAGGCGTA TAAGCTGATC TGTGAGATGA TGACCAAACA TCAAGACGTG
    CTGCCCAACA GTGACTTCCT GGTGCATCTA TACCATGTCA TGCACAAAGG GTTCACAAGC
    GACGATCAGG ATGTGTTGAA CACAATGATC AGGTGGTGCT CTCCTCGCTT CTTCTTTCTG
    GGTCTGCCGG GCTTCACCAT GCTAGTGGGA GACTTCATCA CCGCGGCTGC TCGCATCCTC
    AACACAGACT CCTTTGAGGC TCCACGAGTC GAGGCTCAGG CTGTTCTTGG TTCGCTGGTG
    TGTTTTCCGA ACTTGTATCA GCAGATTCCC TCTCTTCAGT CTGTTCCTGG ATCTGAGGAT
    ATCCTCGTTG GCAAAGAGGA CATGAAGGAC TATCTTGTGA ACATTCTTTT GAAGACTGCA
    CGCAGTGAGA CATGTGAAGG AGCCAGGTGT GTGGCGATCT GCGGTCTGGG TTTGTGGGTG
    TGTGAGGAGC TCAGGCAAAC AGAAACCCAT CATCAGGTCA AAGATGCCAT CAACGTCCTC
    GGTGTCACGC TCAAGTTTGG GAATAAAGTG GTTGCCAACG TGGCGTGCGA CATCTTTCAG
    CTACTGATTT CTCACTGGCA GCACTTACAA AGACTAGAAC CTTCTCTTCC CAAAAGAATC
    ATAGAGATTT TTGTAGCAAC CGTAGCCTTT CTTCTGCCCA GCGCTGAACA CTCAACTGTG
    GAGGCAGACA AGAAGTTAAT GGTGTCTCTA CTGCTGTGCC TGCTGGACTG GTGCATGTCT
    GTACCTCTCT CCATCCTGCT GGAGCCCATC ACTATGCCTG TGCTGGACGA CCCCACCTCC
    CAAAAGGCCC CGCTGCTCGA CTACATTTAC AGGGTGCTTC ACACTTGTGT ATCAGGGTCT
    AACCTGCACA CTCAGCAGAG TCACTACCTC CTCAGCCTGG CTGATCTATC AACAGACTAC
    GAACCCTTCC TTATGCTGGG CAATGTCAAG AGCTTTGAAC CACCGGCCAT ACACAACGCC
    ATGGGAGATT TTGGAAATCT CCTCACTGTG GCTGAAGAAA AGAAAAGGAG AAGCATGGAA
    CTGATACCCC TAACTGCTCG CATGGTAATG ACACACCTGG TCAATCATTT GGGCCACCAT
    CCTCTGAGTG GCGGCCCGGC CCTCTTGAAC AGCCTGATCA GCGAGAACCA CGACAACCCC
    TATGTAGAGT CTTCAGAGCT CTCATCTGAG GTCTTCAAAA GCCCCAATCT GCAGCTCTTC
    GTCTTTAATG ACAGCACGCT CATTTCATAC CTGCAGATCC CCTCTGATAA TTCCAACACT
    GCTCCGCTAA GTGCCAACCC CAGGGATCAT TCCTCTGAGG TCCGCATCAT CATTCGGGAC
    ATCTCAGGCA AATACTCATG GGACAGTGGC GTTCTCTTCC GCACTTTAGA TGGCACCGGT
    GTGACTTCCA GCGACAGCAG AGAACAGTCT TCTCATGAAG CTGCTCGATT AGAAAAGCGG
    AGCTCTGTCC GCTTCAGTAA ATGTTCATCA GAGCTGGACG TGGAAGATGG CGTTGATGTG
    CTGGACCAGC TCCTGGAGGA CCTCGGCCAC AGCAGTCCCG AGTGTCTGCC GGAACCCCAG
    CTCAGACTGA CCCAGCCACC ATCTCCACCC ATGGGCATGA ACCTGGAGAT AGAAGGGCTC
    ATCATGGAGG CCGTCCACCG ACAGTCAAAA CAGGAGGAAG AGGAGTTGAT GCGAAGGCAA
    ATCGAAGACC CCAGTGTCAG AGCAGAGAGG CAGAAAGAGC CCACACATCA GGAGCCCAAA
    TCCCCTTTCT ACCTATGCAG ACTCCTCCTC AATGACCTCG GCATGAACTC ATGGGATCGC
    AGAAAGAGCT TTCATCTCCT GAAGAAGAAC TCCAAGCTTT TAAGAGAGCT GAAGAATTTG
    GACTCGAGGC AGTGTCGAGA GACACACAAG ATAGCAGTCT TCTATATTGG AGAAGGACAA
    GAGGACAAAT ATTCCATTCT GTCCAATACA CAGGGCAGTC AGGTTTATGA GGACTTTGTC
    TCTGGACTGG GATGGGAGGT GAATCTGGCC ACTCACTGTG GGTTTATGGG TGGTTTGCAG
    AGAAATGGCA GCACAGGAAG CACAGCTCCA TATTACGCTA CCTCCAATGT GGAGGTCATC
    TTCCACGTGT CCACTCGGAT GCCTTCTGAC TCTGATGACT CCATCACTAA AAAGCTTCGT
    CACTTGGGAA ACGACGAAGT GCACATCGTG TGGTCGGAGC ACACGCGGGA TTACAGGCGA
    GGAATCATCC CCACCGACTT TGGAGATGTC CTCGTCATCA TCTACCCCAT GAAAAACCAC
    ATGTTCTTTG TTCAGATCAT GAAAAAACCC CAGGTTCCTT TTTTTGGGCC TCTGTTTGAT
    GGGGCGATAG TGACTGGAGA GTTGTTGCCG AGTTTGGTGC GAGCCACGTG CATCAATGCC
    AGCAGGGCCG TGAAGTCTCG TCTGCCACTC TACCAGAGTT TTTATGAAGA AAGAGCGCTG
    TATCTGGAGG CCATCATTCA GAACCACAAG GAGAACATGA CCTTCGAGGA CTTCGCTGCT
    CAGGTCTTCT CTCCATCTCC CAGCTACTGG CCTTCTGGAG GTGCAGGATC CCTCAGCTGC
    AGCACGAGCG CTGACCTCAG TGTTTCTGTG ATGACCAATG ACTCTGGTGA CCAGGTTTCA
    CCCACAATAC CACGCGCTAC CAAGAACCGG GTTTCTGGAA AACTGCGGCG CTCAGCTAGT
    GCCATCAGCA AATCCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158963.1
    CDS115..6018
    Translation

    Target ORF information:

    RefSeq Version XM_005158906.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158906.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGATGTG
    GAAGAGTGTC AGCTGTCGGA GTGTGAGATG GGTGATGGTG ACAGTCCTCT GCCCCGCAGC
    AGCAGCACCT CTGACATCAC ACAGCAAATC AACGACACCT TTACAGGTCA GAGGAAAGAT
    GAGAGTATGG ATCTGGTCAG ATCATCTGTA TCGGAGAGCA GCTCTGCAGG GACGAGAGAA
    AGTGACGCTC CAGTGATTCT GATTGAGCGA AGCAACAGTC CAGCAGATCT GGATTATCCA
    GCTGAAGGAG CTCCAGCTTT CTTGAAGATG AAACTCAGAG AGAAAAGTGA GAGTGTGAGC
    AGTGAGACGT CCAATGGCTA TCTGAATGAG GCAGAAGTGG GTCTGCTGTC GTGGCACACG
    CTGGAGGAAG AGCCGGACTC TCCCTCGACT CAAGACGTGA GCATGAGCAT GAGTGTTACA
    GCAGACACGG TGAGCCAGAG GAGTCTCCTG CTCAGCCACA CTGAAGCACT GGCAGGGTCT
    CCTCACTTTC CCCCAGTGTC TCCGGCTCTT CTGGTGCCTC ATGGCTGCTG TGAGGGCCCT
    CAGCTCCTGG AGGATGCCAT GAACATGCCT CAGCATCTGG ACAGCAGTGA GTTTCTGGCT
    GATGACATCA GCATTATCGC CGGTGGCAGT TTGACTGGTT GGCATGCGGA CTCGGCCTTC
    GTTCTCTGGA GGAGGATTCT GGGTATTCTT GGTGATGTGA ACAGCATCCG CTGTGCCCGC
    ATCCACGCCA AGGTCTTCAG TTACCTCTAT GAGCTCTGGC ACAAGCTGGC CAAGATCAGA
    GATAATCTTG GGATCAGAGT GGATTCAGAC ACAGCCATGG TCAAACCCTT GTTCATCCCT
    CCTCTGCGTA TGTTGGCCTC CTGGCTCTTT AAGGCGACCA TGCTGCCTTC AGAATTTAAA
    GCTGGGAAAC TACAGGCGTA TAAGCTGATC TGTGAGATGA TGACCAAACA TCAAGACGTG
    CTGCCCAACA GTGACTTCCT GGTGCATCTA TACCATGTCA TGCACAAAGG GTTCACAAGC
    GACGATCAGG ATGTGTTGAA CACAATGATC AGGTGGTGCT CTCCTCGCTT CTTCTTTCTG
    GGTCTGCCGG GCTTCACCAT GCTAGTGGGA GACTTCATCA CCGCGGCTGC TCGCATCCTC
    AACACAGACT CCTTTGAGGC TCCACGAGTC GAGGCTCAGG CTGTTCTTGG TTCGCTGGTG
    TGTTTTCCGA ACTTGTATCA GCAGATTCCC TCTCTTCAGT CTGTTCCTGG ATCTGAGGAT
    ATCCTCGTTG GCAAAGAGGA CATGAAGGAC TATCTTGTGA ACATTCTTTT GAAGACTGCA
    CGCAGTGAGA CATGTGAAGG AGCCAGGTGT GTGGCGATCT GCGGTCTGGG TTTGTGGGTG
    TGTGAGGAGC TCAGGCAAAC AGAAACCCAT CATCAGGTCA AAGATGCCAT CAACGTCCTC
    GGTGTCACGC TCAAGTTTGG GAATAAAGTG GTTGCCAACG TGGCGTGCGA CATCTTTCAG
    CTACTGATTT CTCACTGGCA GCACTTACAA AGACTAGAAC CTTCTCTTCC CAAAAGAATC
    ATAGAGATTT TTGTAGCAAC CGTAGCCTTT CTTCTGCCCA GCGCTGAACA CTCAACTGTG
    GAGGCAGACA AGAAGTTAAT GGTGTCTCTA CTGCTGTGCC TGCTGGACTG GTGCATGTCT
    GTACCTCTCT CCATCCTGCT GGAGCCCATC ACTATGCCTG TGCTGGACGA CCCCACCTCC
    CAAAAGGCCC CGCTGCTCGA CTACATTTAC AGGGTGCTTC ACACTTGTGT ATCAGGGTCT
    AACCTGCACA CTCAGCAGAG TCACTACCTC CTCAGCCTGG CTGATCTATC AACAGACTAC
    GAACCCTTCC TTATGCTGGG CAATGTCAAG AGCTTTGAAC CACCGGCCAT ACACAACGCC
    ATGGGAGATT TTGGAAATCT CCTCACTGTG GCTGAAGAAA AGAAAAGGAG AAGCATGGAA
    CTGATACCCC TAACTGCTCG CATGGTAATG ACACACCTGG TCAATCATTT GGGCCACCAT
    CCTCTGAGTG GCGGCCCGGC CCTCTTGAAC AGCCTGATCA GCGAGAACCA CGACAACCCC
    TATGTAGAGT CTTCAGAGCT CTCATCTGAG GTCTTCAAAA GCCCCAATCT GCAGCTCTTC
    GTCTTTAATG ACAGCACGCT CATTTCATAC CTGCAGATCC CCTCTGATAA TTCCAACACT
    GCTCCGCTAA GTGCCAACCC CAGGGATCAT TCCTCTGAGG TCCGCATCAT CATTCGGGAC
    ATCTCAGGCA AATACTCATG GGACAGTGGC GTTCTCTTCC GCACTTTAGA TGGCACCGGT
    GTGACTTCCA GCGACAGCAG AGAACAGTCT TCTCATGAAG CTGCTCGATT AGAAAAGCGG
    AGCTCTGTCC GCTTCAGTAA ATGTTCATCA GAGCTGGACG TGGAAGATGG CGTTGATGTG
    CTGGACCAGC TCCTGGAGGA CCTCGGCCAC AGCAGTCCCG AGTGTCTGCC GGAACCCCAG
    CTCAGACTGA CCCAGCCACC ATCTCCACCC ATGGGCATGA ACCTGGAGAT AGAAGGGCTC
    ATCATGGAGG CCGTCCACCG ACAGTCAAAA CAGGAGGAAG AGGAGTTGAT GCGAAGGCAA
    ATCGAAGACC CCAGTGTCAG AGCAGAGAGG CAGAAAGAGC CCACACATCA GGAGCCCAAA
    TCCCCTTTCT ACCTATGCAG ACTCCTCCTC AATGACCTCG GCATGAACTC ATGGGATCGC
    AGAAAGAGCT TTCATCTCCT GAAGAAGAAC TCCAAGCTTT TAAGAGAGCT GAAGAATTTG
    GACTCGAGGC AGTGTCGAGA GACACACAAG ATAGCAGTCT TCTATATTGG AGAAGGACAA
    GAGGACAAAT ATTCCATTCT GTCCAATACA CAGGGCAGTC AGGTTTATGA GGACTTTGTC
    TCTGGACTGG GATGGGAGGT GAATCTGGCC ACTCACTGTG GGTTTATGGG TGGTTTGCAG
    AGAAATGGCA GCACAGGAAG CACAGCTCCA TATTACGCTA CCTCCAATGT GGAGGTCATC
    TTCCACGTGT CCACTCGGAT GCCTTCTGAC TCTGATGACT CCATCACTAA AAAGCTTCGT
    CACTTGGGAA ACGACGAAGT GCACATCGTG TGGTCGGAGC ACACGCGGGA TTACAGGCGA
    GGAATCATCC CCACCGACTT TGGAGATGTC CTCGTCATCA TCTACCCCAT GAAAAACCAC
    ATGTTCTTTG TTCAGATCAT GAAAAAACCC CAGGTTCCTT TTTTTGGGCC TCTGTTTGAT
    GGGGCGATAG TGACTGGAGA GTTGTTGCCG AGTTTGGTGC GAGCCACGTG CATCAATGCC
    AGCAGGGCCG TGAAGTCTCG TCTGCCACTC TACCAGAGTT TTTATGAAGA AAGAGCGCTG
    TATCTGGAGG CCATCATTCA GAACCACAAG GAGAACATGA CCTTCGAGGA CTTCGCTGCT
    CAGGTCTTCT CTCCATCTCC CAGCTACTGG CCTTCTGGAG GTGCAGGATC CCTCAGCTGC
    AGCACGAGCG CTGACCTCAG TGTTTCTGTG ATGACCAATG ACTCTGGTGA CCAGGTTTCA
    CCCACAATAC CACGCGCTAC CAAGAACCGG GTTTCTGGAA AACTGCGGCG CTCAGCTAGT
    GCCATCAGCA AATCCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22783
    Clone ID Related Accession (Same CDS sequence) XM_005158906.3 , XM_005158906.4
    Accession Version XM_005158906.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGATGTG
    GAAGAGTGTC AGCTGTCGGA GTGTGAGATG GGTGATGGTG ACAGTCCTCT GCCCCGCAGC
    AGCAGCACCT CTGACATCAC ACAGCAAATC AACGACACCT TTACAGGTCA GAGGAAAGAT
    GAGAGTATGG ATCTGGTCAG ATCATCTGTA TCGGAGAGCA GCTCTGCAGG GACGAGAGAA
    AGTGACGCTC CAGTGATTCT GATTGAGCGA AGCAACAGTC CAGCAGATCT GGATTATCCA
    GCTGAAGGAG CTCCAGCTTT CTTGAAGATG AAACTCAGAG AGAAAAGTGA GAGTGTGAGC
    AGTGAGACGT CCAATGGCTA TCTGAATGAG GCAGAAGTGG GTCTGCTGTC GTGGCACACG
    CTGGAGGAAG AGCCGGACTC TCCCTCGACT CAAGACGTGA GCATGAGCAT GAGTGTTACA
    GCAGACACGG TGAGCCAGAG GAGTCTCCTG CTCAGCCACA CTGAAGCACT GGCAGGGTCT
    CCTCACTTTC CCCCAGTGTC TCCGGCTCTT CTGGTGCCTC ATGGCTGCTG TGAGGGCCCT
    CAGCTCCTGG AGGATGCCAT GAACATGCCT CAGCATCTGG ACAGCAGTGA GTTTCTGGCT
    GATGACATCA GCATTATCGC CGGTGGCAGT TTGACTGGTT GGCATGCGGA CTCGGCCTTC
    GTTCTCTGGA GGAGGATTCT GGGTATTCTT GGTGATGTGA ACAGCATCCG CTGTGCCCGC
    ATCCACGCCA AGGTCTTCAG TTACCTCTAT GAGCTCTGGC ACAAGCTGGC CAAGATCAGA
    GATAATCTTG GGATCAGAGT GGATTCAGAC ACAGCCATGG TCAAACCCTT GTTCATCCCT
    CCTCTGCGTA TGTTGGCCTC CTGGCTCTTT AAGGCGACCA TGCTGCCTTC AGAATTTAAA
    GCTGGGAAAC TACAGGCGTA TAAGCTGATC TGTGAGATGA TGACCAAACA TCAAGACGTG
    CTGCCCAACA GTGACTTCCT GGTGCATCTA TACCATGTCA TGCACAAAGG GTTCACAAGC
    GACGATCAGG ATGTGTTGAA CACAATGATC AGGTGGTGCT CTCCTCGCTT CTTCTTTCTG
    GGTCTGCCGG GCTTCACCAT GCTAGTGGGA GACTTCATCA CCGCGGCTGC TCGCATCCTC
    AACACAGACT CCTTTGAGGC TCCACGAGTC GAGGCTCAGG CTGTTCTTGG TTCGCTGGTG
    TGTTTTCCGA ACTTGTATCA GCAGATTCCC TCTCTTCAGT CTGTTCCTGG ATCTGAGGAT
    ATCCTCGTTG GCAAAGAGGA CATGAAGGAC TATCTTGTGA ACATTCTTTT GAAGACTGCA
    CGCAGTGAGA CATGTGAAGG AGCCAGGTGT GTGGCGATCT GCGGTCTGGG TTTGTGGGTG
    TGTGAGGAGC TCAGGCAAAC AGAAACCCAT CATCAGGTCA AAGATGCCAT CAACGTCCTC
    GGTGTCACGC TCAAGTTTGG GAATAAAGTG GTTGCCAACG TGGCGTGCGA CATCTTTCAG
    CTACTGATTT CTCACTGGCA GCACTTACAA AGACTAGAAC CTTCTCTTCC CAAAAGAATC
    ATAGAGATTT TTGTAGCAAC CGTAGCCTTT CTTCTGCCCA GCGCTGAACA CTCAACTGTG
    GAGGCAGACA AGAAGTTAAT GGTGTCTCTA CTGCTGTGCC TGCTGGACTG GTGCATGTCT
    GTACCTCTCT CCATCCTGCT GGAGCCCATC ACTATGCCTG TGCTGGACGA CCCCACCTCC
    CAAAAGGCCC CGCTGCTCGA CTACATTTAC AGGGTGCTTC ACACTTGTGT ATCAGGGTCT
    AACCTGCACA CTCAGCAGAG TCACTACCTC CTCAGCCTGG CTGATCTATC AACAGACTAC
    GAACCCTTCC TTATGCTGGG CAATGTCAAG AGCTTTGAAC CACCGGCCAT ACACAACGCC
    ATGGGAGATT TTGGAAATCT CCTCACTGTG GCTGAAGAAA AGAAAAGGAG AAGCATGGAA
    CTGATACCCC TAACTGCTCG CATGGTAATG ACACACCTGG TCAATCATTT GGGCCACCAT
    CCTCTGAGTG GCGGCCCGGC CCTCTTGAAC AGCCTGATCA GCGAGAACCA CGACAACCCC
    TATGTAGAGT CTTCAGAGCT CTCATCTGAG GTCTTCAAAA GCCCCAATCT GCAGCTCTTC
    GTCTTTAATG ACAGCACGCT CATTTCATAC CTGCAGATCC CCTCTGATAA TTCCAACACT
    GCTCCGCTAA GTGCCAACCC CAGGGATCAT TCCTCTGAGG TCCGCATCAT CATTCGGGAC
    ATCTCAGGCA AATACTCATG GGACAGTGGC GTTCTCTTCC GCACTTTAGA TGGCACCGGT
    GTGACTTCCA GCGACAGCAG AGAACAGTCT TCTCATGAAG CTGCTCGATT AGAAAAGCGG
    AGCTCTGTCC GCTTCAGTAA ATGTTCATCA GAGCTGGACG TGGAAGATGG CGTTGATGTG
    CTGGACCAGC TCCTGGAGGA CCTCGGCCAC AGCAGTCCCG AGTGTCTGCC GGAACCCCAG
    CTCAGACTGA CCCAGCCACC ATCTCCACCC ATGGGCATGA ACCTGGAGAT AGAAGGGCTC
    ATCATGGAGG CCGTCCACCG ACAGTCAAAA CAGGAGGAAG AGGAGTTGAT GCGAAGGCAA
    ATCGAAGACC CCAGTGTCAG AGCAGAGAGG CAGAAAGAGC CCACACATCA GGAGCCCAAA
    TCCCCTTTCT ACCTATGCAG ACTCCTCCTC AATGACCTCG GCATGAACTC ATGGGATCGC
    AGAAAGAGCT TTCATCTCCT GAAGAAGAAC TCCAAGCTTT TAAGAGAGCT GAAGAATTTG
    GACTCGAGGC AGTGTCGAGA GACACACAAG ATAGCAGTCT TCTATATTGG AGAAGGACAA
    GAGGACAAAT ATTCCATTCT GTCCAATACA CAGGGCAGTC AGGTTTATGA GGACTTTGTC
    TCTGGACTGG GATGGGAGGT GAATCTGGCC ACTCACTGTG GGTTTATGGG TGGTTTGCAG
    AGAAATGGCA GCACAGGAAG CACAGCTCCA TATTACGCTA CCTCCAATGT GGAGGTCATC
    TTCCACGTGT CCACTCGGAT GCCTTCTGAC TCTGATGACT CCATCACTAA AAAGCTTCGT
    CACTTGGGAA ACGACGAAGT GCACATCGTG TGGTCGGAGC ACACGCGGGA TTACAGGCGA
    GGAATCATCC CCACCGACTT TGGAGATGTC CTCGTCATCA TCTACCCCAT GAAAAACCAC
    ATGTTCTTTG TTCAGATCAT GAAAAAACCC CAGGTTCCTT TTTTTGGGCC TCTGTTTGAT
    GGGGCGATAG TGACTGGAGA GTTGTTGCCG AGTTTGGTGC GAGCCACGTG CATCAATGCC
    AGCAGGGCCG TGAAGTCTCG TCTGCCACTC TACCAGAGTT TTTATGAAGA AAGAGCGCTG
    TATCTGGAGG CCATCATTCA GAACCACAAG GAGAACATGA CCTTCGAGGA CTTCGCTGCT
    CAGGTCTTCT CTCCATCTCC CAGCTACTGG CCTTCTGGAG GTGCAGGATC CCTCAGCTGC
    AGCACGAGCG CTGACCTCAG TGTTTCTGTG ATGACCAATG ACTCTGGTGA CCAGGTTTCA
    CCCACAATAC CACGCGCTAC CAAGAACCGG GTTTCTGGAA AACTGCGGCG CTCAGCTAGT
    GCCATCAGCA AATCCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158963.1
    CDS131..6034
    Translation

    Target ORF information:

    RefSeq Version XM_005158906.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158906.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGATGTG
    GAAGAGTGTC AGCTGTCGGA GTGTGAGATG GGTGATGGTG ACAGTCCTCT GCCCCGCAGC
    AGCAGCACCT CTGACATCAC ACAGCAAATC AACGACACCT TTACAGGTCA GAGGAAAGAT
    GAGAGTATGG ATCTGGTCAG ATCATCTGTA TCGGAGAGCA GCTCTGCAGG GACGAGAGAA
    AGTGACGCTC CAGTGATTCT GATTGAGCGA AGCAACAGTC CAGCAGATCT GGATTATCCA
    GCTGAAGGAG CTCCAGCTTT CTTGAAGATG AAACTCAGAG AGAAAAGTGA GAGTGTGAGC
    AGTGAGACGT CCAATGGCTA TCTGAATGAG GCAGAAGTGG GTCTGCTGTC GTGGCACACG
    CTGGAGGAAG AGCCGGACTC TCCCTCGACT CAAGACGTGA GCATGAGCAT GAGTGTTACA
    GCAGACACGG TGAGCCAGAG GAGTCTCCTG CTCAGCCACA CTGAAGCACT GGCAGGGTCT
    CCTCACTTTC CCCCAGTGTC TCCGGCTCTT CTGGTGCCTC ATGGCTGCTG TGAGGGCCCT
    CAGCTCCTGG AGGATGCCAT GAACATGCCT CAGCATCTGG ACAGCAGTGA GTTTCTGGCT
    GATGACATCA GCATTATCGC CGGTGGCAGT TTGACTGGTT GGCATGCGGA CTCGGCCTTC
    GTTCTCTGGA GGAGGATTCT GGGTATTCTT GGTGATGTGA ACAGCATCCG CTGTGCCCGC
    ATCCACGCCA AGGTCTTCAG TTACCTCTAT GAGCTCTGGC ACAAGCTGGC CAAGATCAGA
    GATAATCTTG GGATCAGAGT GGATTCAGAC ACAGCCATGG TCAAACCCTT GTTCATCCCT
    CCTCTGCGTA TGTTGGCCTC CTGGCTCTTT AAGGCGACCA TGCTGCCTTC AGAATTTAAA
    GCTGGGAAAC TACAGGCGTA TAAGCTGATC TGTGAGATGA TGACCAAACA TCAAGACGTG
    CTGCCCAACA GTGACTTCCT GGTGCATCTA TACCATGTCA TGCACAAAGG GTTCACAAGC
    GACGATCAGG ATGTGTTGAA CACAATGATC AGGTGGTGCT CTCCTCGCTT CTTCTTTCTG
    GGTCTGCCGG GCTTCACCAT GCTAGTGGGA GACTTCATCA CCGCGGCTGC TCGCATCCTC
    AACACAGACT CCTTTGAGGC TCCACGAGTC GAGGCTCAGG CTGTTCTTGG TTCGCTGGTG
    TGTTTTCCGA ACTTGTATCA GCAGATTCCC TCTCTTCAGT CTGTTCCTGG ATCTGAGGAT
    ATCCTCGTTG GCAAAGAGGA CATGAAGGAC TATCTTGTGA ACATTCTTTT GAAGACTGCA
    CGCAGTGAGA CATGTGAAGG AGCCAGGTGT GTGGCGATCT GCGGTCTGGG TTTGTGGGTG
    TGTGAGGAGC TCAGGCAAAC AGAAACCCAT CATCAGGTCA AAGATGCCAT CAACGTCCTC
    GGTGTCACGC TCAAGTTTGG GAATAAAGTG GTTGCCAACG TGGCGTGCGA CATCTTTCAG
    CTACTGATTT CTCACTGGCA GCACTTACAA AGACTAGAAC CTTCTCTTCC CAAAAGAATC
    ATAGAGATTT TTGTAGCAAC CGTAGCCTTT CTTCTGCCCA GCGCTGAACA CTCAACTGTG
    GAGGCAGACA AGAAGTTAAT GGTGTCTCTA CTGCTGTGCC TGCTGGACTG GTGCATGTCT
    GTACCTCTCT CCATCCTGCT GGAGCCCATC ACTATGCCTG TGCTGGACGA CCCCACCTCC
    CAAAAGGCCC CGCTGCTCGA CTACATTTAC AGGGTGCTTC ACACTTGTGT ATCAGGGTCT
    AACCTGCACA CTCAGCAGAG TCACTACCTC CTCAGCCTGG CTGATCTATC AACAGACTAC
    GAACCCTTCC TTATGCTGGG CAATGTCAAG AGCTTTGAAC CACCGGCCAT ACACAACGCC
    ATGGGAGATT TTGGAAATCT CCTCACTGTG GCTGAAGAAA AGAAAAGGAG AAGCATGGAA
    CTGATACCCC TAACTGCTCG CATGGTAATG ACACACCTGG TCAATCATTT GGGCCACCAT
    CCTCTGAGTG GCGGCCCGGC CCTCTTGAAC AGCCTGATCA GCGAGAACCA CGACAACCCC
    TATGTAGAGT CTTCAGAGCT CTCATCTGAG GTCTTCAAAA GCCCCAATCT GCAGCTCTTC
    GTCTTTAATG ACAGCACGCT CATTTCATAC CTGCAGATCC CCTCTGATAA TTCCAACACT
    GCTCCGCTAA GTGCCAACCC CAGGGATCAT TCCTCTGAGG TCCGCATCAT CATTCGGGAC
    ATCTCAGGCA AATACTCATG GGACAGTGGC GTTCTCTTCC GCACTTTAGA TGGCACCGGT
    GTGACTTCCA GCGACAGCAG AGAACAGTCT TCTCATGAAG CTGCTCGATT AGAAAAGCGG
    AGCTCTGTCC GCTTCAGTAA ATGTTCATCA GAGCTGGACG TGGAAGATGG CGTTGATGTG
    CTGGACCAGC TCCTGGAGGA CCTCGGCCAC AGCAGTCCCG AGTGTCTGCC GGAACCCCAG
    CTCAGACTGA CCCAGCCACC ATCTCCACCC ATGGGCATGA ACCTGGAGAT AGAAGGGCTC
    ATCATGGAGG CCGTCCACCG ACAGTCAAAA CAGGAGGAAG AGGAGTTGAT GCGAAGGCAA
    ATCGAAGACC CCAGTGTCAG AGCAGAGAGG CAGAAAGAGC CCACACATCA GGAGCCCAAA
    TCCCCTTTCT ACCTATGCAG ACTCCTCCTC AATGACCTCG GCATGAACTC ATGGGATCGC
    AGAAAGAGCT TTCATCTCCT GAAGAAGAAC TCCAAGCTTT TAAGAGAGCT GAAGAATTTG
    GACTCGAGGC AGTGTCGAGA GACACACAAG ATAGCAGTCT TCTATATTGG AGAAGGACAA
    GAGGACAAAT ATTCCATTCT GTCCAATACA CAGGGCAGTC AGGTTTATGA GGACTTTGTC
    TCTGGACTGG GATGGGAGGT GAATCTGGCC ACTCACTGTG GGTTTATGGG TGGTTTGCAG
    AGAAATGGCA GCACAGGAAG CACAGCTCCA TATTACGCTA CCTCCAATGT GGAGGTCATC
    TTCCACGTGT CCACTCGGAT GCCTTCTGAC TCTGATGACT CCATCACTAA AAAGCTTCGT
    CACTTGGGAA ACGACGAAGT GCACATCGTG TGGTCGGAGC ACACGCGGGA TTACAGGCGA
    GGAATCATCC CCACCGACTT TGGAGATGTC CTCGTCATCA TCTACCCCAT GAAAAACCAC
    ATGTTCTTTG TTCAGATCAT GAAAAAACCC CAGGTTCCTT TTTTTGGGCC TCTGTTTGAT
    GGGGCGATAG TGACTGGAGA GTTGTTGCCG AGTTTGGTGC GAGCCACGTG CATCAATGCC
    AGCAGGGCCG TGAAGTCTCG TCTGCCACTC TACCAGAGTT TTTATGAAGA AAGAGCGCTG
    TATCTGGAGG CCATCATTCA GAACCACAAG GAGAACATGA CCTTCGAGGA CTTCGCTGCT
    CAGGTCTTCT CTCCATCTCC CAGCTACTGG CCTTCTGGAG GTGCAGGATC CCTCAGCTGC
    AGCACGAGCG CTGACCTCAG TGTTTCTGTG ATGACCAATG ACTCTGGTGA CCAGGTTTCA
    CCCACAATAC CACGCGCTAC CAAGAACCGG GTTTCTGGAA AACTGCGGCG CTCAGCTAGT
    GCCATCAGCA AATCCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22784
    Clone ID Related Accession (Same CDS sequence) XM_005158907.3 , XM_005158907.4
    Accession Version XM_005158907.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158964.1
    CDS115..6009
    Translation

    Target ORF information:

    RefSeq Version XM_005158907.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158907.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22784
    Clone ID Related Accession (Same CDS sequence) XM_005158907.3 , XM_005158907.4
    Accession Version XM_005158907.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158907.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158964.1
    CDS132..6026
    Translation

    Target ORF information:

    RefSeq Version XM_005158907.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158907.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45361
    Clone ID Related Accession (Same CDS sequence) XM_009293239.2
    Accession Version XM_009293239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG CAGGCAGGCG TTGGCCAGGG ATGCTTTGGG ACGTGGATTA CCTGTGGGCC
    AAGCTGGACT GTAAGACTTT TTCTGCCTGC TTCTTCCATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291514.1
    CDS115..6096
    Translation

    Target ORF information:

    RefSeq Version XM_009293239.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG CAGGCAGGCG TTGGCCAGGG ATGCTTTGGG ACGTGGATTA CCTGTGGGCC
    AAGCTGGACT GTAAGACTTT TTCTGCCTGC TTCTTCCATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22779
    Clone ID Related Accession (Same CDS sequence) XM_001920473.5 , XM_001920473.6
    Accession Version XM_001920473.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001920473.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GATCCCTCAG CTGCAGCACG AGCGCTGACC TCAGTGTTTC TGTGATGACC
    AATGACTCTG GTGACCAGGT TTCACCCACA ATACCACGCG CTACCAAGAA CCGGGTTTCT
    GGAAAACTGC GGCGCTCAGC TAGTGCCATC AGCAAATCCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001920508.2
    CDS115..6162
    Translation

    Target ORF information:

    RefSeq Version XM_001920473.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001920473.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GATCCCTCAG CTGCAGCACG AGCGCTGACC TCAGTGTTTC TGTGATGACC
    AATGACTCTG GTGACCAGGT TTCACCCACA ATACCACGCG CTACCAAGAA CCGGGTTTCT
    GGAAAACTGC GGCGCTCAGC TAGTGCCATC AGCAAATCCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22779
    Clone ID Related Accession (Same CDS sequence) XM_001920473.5 , XM_001920473.6
    Accession Version XM_001920473.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ral GTPase-activating protein subunit alpha-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001920473.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GATCCCTCAG CTGCAGCACG AGCGCTGACC TCAGTGTTTC TGTGATGACC
    AATGACTCTG GTGACCAGGT TTCACCCACA ATACCACGCG CTACCAAGAA CCGGGTTTCT
    GGAAAACTGC GGCGCTCAGC TAGTGCCATC AGCAAATCCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001920508.2
    CDS131..6178
    Translation

    Target ORF information:

    RefSeq Version XM_001920473.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ral GTPase activating protein, alpha subunit 2 (catalytic) (ralgapa2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001920473.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTTCACTC GAAGGGTTTA TGGAGACGTC AAGAAATCTA CACAGAAAGT TCTGGACCCA 
    AAGAAGGATG TTTTTACGCG TCTGAAGCAT CTGCGCGCGC TGTTAGATAT TATCGACAGG
    AATGAACTGA AGGCTTTTTT TGAAACCAAC TGCTCACAGA TCTATTTTAT CTTCTATGAA
    AATTTCATTA CACTGGAAAG TAACTTAAAA CAAAAAGGGA ACAAATCTCA ACGGGAGGAG
    CTGGACTCCA TCCTCTTTAT TTTTGAAAAA ATCCTGCAAC TTCTACCTGA GAAAATCAAC
    AGTCGATGGC AGTATCACAG TATAGGTTCT ATACTCAAAA AACTTTTGCA CACTGGAAAC
    TCTTTTAAGA TCCGCTGTGA GGGCATGCGT CTGTTCCTGC TCTGGCTGCA GGCTTTACAG
    AATCACTGTG CTGAAGAGCA GCTTCTCATC TTCGCCTGTC TGATCCCAGG ATTCCCTGCT
    GTACCCTCAT CTAGAGGACC CTGCACACTG GACACCATCA TATACAACCC CTTCTCCAAC
    CCACCCGATG CTAAGATTGT GCCAGAGGAG ATCACTCCGC TGGTGCCTGC AGTTCCTGGA
    GAGAAGGTGG CGGACGACCA CACCTGCTCT GTCCTGCAGA TCGTGCTGAA GGTCATAGTT
    CTCCAGGCAT CCAGCTTGGA CTGGAAAAAC AAAGAGAACC AGGACACAGG CTTCAGATTT
    CTCTTTTCCC TCTTCAAGAA ATACTACCTG CCTCACCTCT TCCCCACTTT CTCAAAACAT
    ACCAACCTTT ACAAGCCACA GTTAGATCTT CCCATTCACA GACCAAAGCC CTTGTATGTT
    CCAGTGACTC GTAACAATGA GAGCACATAC TGCACTAGGG ATCAGTATCT GGCTCCTCGC
    GTGGCCTTCA TCACGTGGCT CGTCAACTTC TTCCTGGAGA AGAAGTATGT GAACTCGACC
    ACGAGCAACT CCAAGAATGG AGGAGAGGTC TTGCCTAAAC TCATCCAGAC TGTGACTGCA
    GGCAGTATCA GTCAGGAGAA GAGCACAGAC TTAGAGCCCA ACGGTCCTGT GGAGCAGGAG
    AAGAATCACT CTAACAGCAG CACCCTGTCT GACCGCAGGG GCAGCAACTC CAGCCTGTGC
    AGCATCGAGG AGGAGCACCG CGGCGTCTAC GACATGGTGC AGGCCATCCT GCTCTCCACA
    CGGGACAACG TCAATTTCGT CAATGAGGTC TTCCATCAGG CTTTCCTGCT GCCATCCTGT
    GAAGCTTCGG CCACTCGCAA GGTGATCAAG GTGTACAGGA AGTGGATTCT ACAGGAGAAA
    CCTGCTTTCA TGGCTGAGCC TGAGACCACA AACCAGGAGG AAGATACAGA GGAAGAGCAG
    CTGGTCACAG AGACGGACAA TGCACACATA CAGGCCCTGG AAACTCATGG ACACAAGAGA
    TCGTCCAGCT GGGGGCGCAC TTACTCCTTC AGCAGCGCCA TCAGCAGAGG CTGCCTGACA
    GAGGAGCTCA ACCTGGAAGT GAAGGCTGGT ATCCAGTCTG CCCTACAGGT GTTTCTGACA
    AATTCTGCAA ACGTGTTTCT GTTGGAGCCG TGTCAGGATG TGCCCAAACT GCTGGAAAAT
    CAGCTGGAAG TGTGCAGAGC TGTGCTCAGC GTCTACCGCC ACATAATTAT GGAACAAAAC
    ATGAACCGGC AGACCTGGGA GCAGTTGCTG CAGGTGTTGC TGAGAATCAC GGAAGCAGTG
    ATGAAGAGAC CGCAGGAGAA CCAAAAGAAA GACACGTTCG CCCACAGCCT AGCTAGCATC
    CTCTTCAAAA CGATCTTTGT AGCGTGGGTT CGGGCCAACC TGACTGTGTT CATCTCCCGG
    GAGCTGTGGG ACGAGCTGCT GGCTGTGCTG TCCTCTCTTA GCCACTGGGA GGAGCTGGTG
    TTCGAGTGGG CCAGCATCAT GGACTCCCTC ACTGCGGTCC TGGCCCGTCA CGTTTACGGC
    CTGGACCTCC ACAACCTGCC GCTGGACAAA CTCTCTGAGC AGAAGGAGAA GAAGCAGAGG
    GGAAGAGGAG TGATGGCGGA TTCTCACAAG GTCTCAGAAG CGGGACGCTC TTTCTCCTTG
    AGCTGGAAGA ACTCTGGAGA TCAGGTTGGC GCTCAGGAGC CCATGAGGTT CCGCAGCGCC
    ACGACCACTG GAACTCCAGC TGTAGAGAAA GCTCGCAACA ACGTCAGGCA GAAAGCAAAG
    CGAAGTCAGT CTATCAGCAA CTGTGTGCAT CTTTGTGAGG CTTTGCCGGC CACTAAGAGT
    GTGCCCATGC TGCTCCACAC TGTCAGCTCT TTCTTACCTG GCATCTCTCA CAACTCTCAC
    TGCTCTCACA GACTCTCAGA TGTGGAAGAG TGTCAGCTGT CGGAGTGTGA GATGGGTGAT
    GGTGACAGTC CTCTGCCCCG CAGCAGCAGC ACCTCTGACA TCACACAGCA AATCAACGAC
    ACCTTTACAG GTCAGAGGAA AGATGAGAGT ATGGATCTGG TCAGATCATC TGTATCGGAG
    AGCAGCTCTG CAGGGACGAG AGAAAGTGAC GCTCCAGTGA TTCTGATTGA GCGAAGCAAC
    AGTCCAGCAG ATCTGGATTA TCCAGCTGAA GGAGCTCCAG CTTTCTTGAA GATGAAACTC
    AGAGAGAAAA GTGAGAGTGT GAGCAGTGAG ACGTCCAATG GCTATCTGAA TGAGGCAGAA
    GTGGGTCTGC TGTCGTGGCA CACGCTGGAG GAAGAGCCGG ACTCTCCCTC GACTCAAGAC
    GTGAGCATGA GCATGAGTGT TACAGCAGAC ACGGTGAGCC AGAGGAGTCT CCTGCTCAGC
    CACACTGAAG CACTGGCAGG GTCTCCTCAC TTTCCCCCAG TGTCTCCGGC TCTTCTGGTG
    CCTCATGGCT GCTGTGAGGG CCCTCAGCTC CTGGAGGATG CCATGAACAT GCCTCAGCAT
    CTGGACAGCA GTGAGTTTCT GGCTGATGAC ATCAGCATTA TCGCCGGTGG CAGTTTGACT
    GGTTGGCATG CGGACTCGGC CTTCGTTCTC TGGAGGAGGA TTCTGGGTAT TCTTGGTGAT
    GTGAACAGCA TCCGCTGTGC CCGCATCCAC GCCAAGGTCT TCAGTTACCT CTATGAGCTC
    TGGCACAAGC TGGCCAAGAT CAGAGATAAT CTTGGGATCA GAGTGGATTC AGACACAGCC
    ATGGTCAAAC CCTTGTTCAT CCCTCCTCTG CGTATGTTGG CCTCCTGGCT CTTTAAGGCG
    ACCATGCTGC CTTCAGAATT TAAAGCTGGG AAACTACAGG CGTATAAGCT GATCTGTGAG
    ATGATGACCA AACATCAAGA CGTGCTGCCC AACAGTGACT TCCTGGTGCA TCTATACCAT
    GTCATGCACA AAGGGTTCAC AAGCGACGAT CAGGATGTGT TGAACACAAT GATCAGGTGG
    TGCTCTCCTC GCTTCTTCTT TCTGGGTCTG CCGGGCTTCA CCATGCTAGT GGGAGACTTC
    ATCACCGCGG CTGCTCGCAT CCTCAACACA GACTCCTTTG AGGCTCCACG AGTCGAGGCT
    CAGGCTGTTC TTGGTTCGCT GGTGTGTTTT CCGAACTTGT ATCAGCAGAT TCCCTCTCTT
    CAGTCTGTTC CTGGATCTGA GGATATCCTC GTTGGCAAAG AGGACATGAA GGACTATCTT
    GTGAACATTC TTTTGAAGAC TGCACGCAGT GAGACATGTG AAGGAGCCAG GTGTGTGGCG
    ATCTGCGGTC TGGGTTTGTG GGTGTGTGAG GAGCTCAGGC AAACAGAAAC CCATCATCAG
    GTCAAAGATG CCATCAACGT CCTCGGTGTC ACGCTCAAGT TTGGGAATAA AGTGGTTGCC
    AACGTGGCGT GCGACATCTT TCAGCTACTG ATTTCTCACT GGCAGCACTT ACAAAGACTA
    GAACCTTCTC TTCCCAAAAG AATCATAGAG ATTTTTGTAG CAACCGTAGC CTTTCTTCTG
    CCCAGCGCTG AACACTCAAC TGTGGAGGCA GACAAGAAGT TAATGGTGTC TCTACTGCTG
    TGCCTGCTGG ACTGGTGCAT GTCTGTACCT CTCTCCATCC TGCTGGAGCC CATCACTATG
    CCTGTGCTGG ACGACCCCAC CTCCCAAAAG GCCCCGCTGC TCGACTACAT TTACAGGGTG
    CTTCACACTT GTGTATCAGG GTCTAACCTG CACACTCAGC AGAGTCACTA CCTCCTCAGC
    CTGGCTGATC TATCAACAGA CTACGAACCC TTCCTTATGC TGGGCAATGT CAAGAGCTTT
    GAACCACCGG CCATACACAA CGCCATGGGA GATTTTGGAA ATCTCCTCAC TGTGGCTGAA
    GAAAAGAAAA GGAGAAGCAT GGAACTGATA CCCCTAACTG CTCGCATGGT AATGACACAC
    CTGGTCAATC ATTTGGGCCA CCATCCTCTG AGTGGCGGCC CGGCCCTCTT GAACAGCCTG
    ATCAGCGAGA ACCACGACAA CCCCTATGTA GAGTCTTCAG AGCTCTCATC TGAGGTCTTC
    AAAAGCCCCA ATCTGCAGCT CTTCGTCTTT AATGACAGCA CGCTCATTTC ATACCTGCAG
    ATCCCCTCTG ATAATTCCAA CACTGCTCCG CTAAGTGCCA ACCCCAGGGA TCATTCCTCT
    GAGGTCCGCA TCATCATTCG GGACATCTCA GGCAAATACT CATGGGACAG TGGCGTTCTC
    TTCCGCACTT TAGATGGCAC CGGTGTGACT TCCAGCGACA GCAGAGAACA GTCTTCTCAT
    GAAGCTGCTC GATTAGAAAA GCGGAGCTCT GTCCGCTTCA GTAAATGTTC ATCAGAGCTG
    GACGTGGAAG ATGGCGTTGA TGTGCTGGAC CAGCTCCTGG AGGACCTCGG CCACAGCAGT
    CCCGAGTGTC TGCCGGAACC CCAGCTCAGA CTGACCCAGC CACCATCTCC ACCCATGGGC
    ATGAACCTGG AGATAGAAGG GCTCATCATG GAGGCCGTCC ACCGACAGTC AAAACAGGAG
    GAAGAGGAGT TGATGCGAAG GCAAATCGAA GACCCCAGTG TCAGAGCAGA GAGGCAGAAA
    GAGCCCACAC ATCAGGAGCC CAAATCCCCT TTCTACCTAT GCAGACTCCT CCTCAATGAC
    CTCGGCATGA ACTCATGGGA TCGCAGAAAG AGCTTTCATC TCCTGAAGAA GAACTCCAAG
    CTTTTAAGAG AGCTGAAGAA TTTGGACTCG AGGCAGTGTC GAGAGACACA CAAGATAGCA
    GTCTTCTATA TTGGAGAAGG ACAAGAGGAC AAATATTCCA TTCTGTCCAA TACACAGGGC
    AGTCAGGTTT ATGAGGACTT TGTCTCTGGA CTGGGATGGG AGGTGAATCT GGCCACTCAC
    TGTGGGTTTA TGGGTGGTTT GCAGAGAAAT GGCAGCACAG GAAGCACAGC TCCATATTAC
    GCTACCTCCA ATGTGGAGGT CATCTTCCAC GTGTCCACTC GGATGCCTTC TGACTCTGAT
    GACTCCATCA CTAAAAAGCT TCGTCACTTG GGAAACGACG AAGTGCACAT CGTGTGGTCG
    GAGCACACGC GGGATTACAG GCGAGGAATC ATCCCCACCG ACTTTGGAGA TGTCCTCGTC
    ATCATCTACC CCATGAAAAA CCACATGTTC TTTGTTCAGA TCATGAAAAA ACCCCAGGTT
    CCTTTTTTTG GGCCTCTGTT TGATGGGGCG ATAGTGACTG GAGAGTTGTT GCCGAGTTTG
    GTGCGAGCCA CGTGCATCAA TGCCAGCAGG GCCGTGAAGT CTCGTCTGCC ACTCTACCAG
    AGTTTTTATG AAGAAAGAGC GCTGTATCTG GAGGCCATCA TTCAGAACCA CAAGGAGAAC
    ATGACCTTCG AGGACTTCGC TGCTCAGGTC TTCTCTCCAT CTCCCAGCTA CTGGCCTTCT
    GGAGGTGCAG GATCCCTCAG CTGCAGCACG AGCGCTGACC TCAGTGTTTC TGTGATGACC
    AATGACTCTG GTGACCAGGT TTCACCCACA ATACCACGCG CTACCAAGAA CCGGGTTTCT
    GGAAAACTGC GGCGCTCAGC TAGTGCCATC AGCAAATCCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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