hace1 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following hace1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hace1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa46293 XM_009292015.2
Latest version!
Danio rerio HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (hace1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa21011 XM_696143.9
Latest version!
Danio rerio HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (hace1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
ODa21011 XM_696143.8 Danio rerio HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (hace1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa46293
    Clone ID Related Accession (Same CDS sequence) XM_009292015.2
    Accession Version XM_009292015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGATGGAAC GGGCGATGGA GCATCTGAAC GTGCAGCTGA ATAGATTGAC GCGCTCTCTG 
    CGTCGCGCCC GGACTGTGGA GCTCCCCGAA GACAGTGAAA CCGCAGTGTA CACCCTCATG
    CCCATGGTCA TGGCTGACCA GCACAGGTCA GTTTCTGAAT TGCTTTTAAA CTCTAAGTTT
    GATGTGAACT ACGCCTTTGG ACGTGTTAAA AGAAGCCTTC TACACATCGC TGCCAATTGT
    GGATCTGTAG AGTGTCTCGT CCTGCTGTTA AAGCGTGGAG CGAATCCGAA CTATCAGGAC
    ATCTCCGGCT GCACACCTCT GCATCTGGCT GCCAGAAATG GGCAGAAGAA ATGTATGGGC
    AGACTGTTAG AGTACAACGC TGATGTTAAT ATCTGCAATA ATGAAGGCCT CACTGCTATT
    CATTGGCTGG CAGTGAACGG ACGGACAGAA TTACTTCATG ACCTGGTGCA GCACGTCACT
    AATGTAGACG TGGAGGATGC CATGGGACAG ACAGCCTTAC ATGTGGCCTG CCAAAACGGA
    CACAAGACTA CAGTGCAGTG TCTTCTGGAC AGTGGAGCAG ACATCAACAG GCCCAATGTG
    TCTGGAGCCA CTCCACTCTA TTTCGCCTGC AGTCACGGTC AGAGGGACAC GGCACAGATT
    CTCCTCCTCA GGGGAGCCAA GTATCTTCCA GACAGGAATG GAGTTACACC ACTCGACTTG
    TGTGTTCAGG GTGGCTATGG CGAGACGTGT GAAATCCTCA TTCAGCATCA TGGCCGACTG
    TTTCAGACGC TGATACAGAT GACCCAGAAT GATGACATTA AAGAGAACAT GCTGAGGCAG
    GTTCTAGAGC ATGTTTCACA GCAGAATGAC TCCAACTACC AAAGGATCTT GACCAGCTTG
    GCGGAAGTGG CCACTACAAA TGGACACAAA CTGCTGAGTT TGTCCAGTAA CTTTGAAGTC
    CAAACCAAGA GTTTACTGCG GATCATTCGG ATCTTCTGCC ATGTGTTCTG TCTTGGACCG
    TCGTCCCCAA ATAATGGGAA TGATATGGGC TACAATGGAA ATAAGACGCC TCGTAGCCAA
    GTGTTTAAGC CTCTGGAGCT GTTATGGCAC TCTCTGGATG AATGGCTGGT GCTCATCTCC
    ACCGAGCTGG AGAAGGAGAT CACAGACACA ACACGCTCCT CCTCAGGAAA TGACATCGCC
    TCTCTCTTCC TGAAAAAGCA GGAAGTCGAC CACTCAGTTT CCAGTGAAAA CCCTCAGCTT
    CTGCTGGATG CCAGTTCTGT GATGAAGACG CCCGAGGTTT ACGCTGACGG TCAGGATGTC
    ATCTCAATGA TCGCCAATCG GCTGAGCGCT GTCATCCAGG CCTTCTACAT GTGCTGCTCC
    TGTCAGATGC CACATGGCAT GACCTCCCCA CGATTCATCG AGTTTGTCTG CAAGCATGAT
    GAAGTGCTGA AGTGTTTTGT GACAAGGAAT CCCAAAATAA TCTTCAACCA CTTCCATTTC
    CTGCTGGAGT GTCCTGAGCT CATGTCCCGC TTCATGCACA TAATCAAGGG GCAGCCGTTT
    AAAGACCGGT GCGAGTGGTT TTATGAACAT CTTCTTGCTG GACAGCCAGA CTCCGATATG
    GTGCATCGAC CGGTTAATGA AAACGACATC CTTCTCGTGC ACAGAGACTC GTTATTCAGA
    AGCAGCTGTG AAGTCGTGTC CAAGTCCTCC AATGAGAAAC TCAAACAAGG CATCGCCGTG
    CGGTTTCACG GTGAAGAGGG AATGGGTCAG GGAGTGGTCA GAGAGTGGTT CGACATTCTT
    TCCAATGAGA TCATCAACCC AGACTATGCA CTCTTCACAC AATCTGCTGA TGGTACCACG
    TTTCAACCCA ACAGTAACTC CTCTGTAAAC CCAGATCATC TGAACTACTT CCGATTTGCT
    GGTCAAATCC TGGGTCTGGC GCTCTATCAT CGACAGCTGG AATCCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290290.1
    CDS209..2179
    Translation

    Target ORF information:

    RefSeq Version XM_009292015.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (hace1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGATGGAAC GGGCGATGGA GCATCTGAAC GTGCAGCTGA ATAGATTGAC GCGCTCTCTG 
    CGTCGCGCCC GGACTGTGGA GCTCCCCGAA GACAGTGAAA CCGCAGTGTA CACCCTCATG
    CCCATGGTCA TGGCTGACCA GCACAGGTCA GTTTCTGAAT TGCTTTTAAA CTCTAAGTTT
    GATGTGAACT ACGCCTTTGG ACGTGTTAAA AGAAGCCTTC TACACATCGC TGCCAATTGT
    GGATCTGTAG AGTGTCTCGT CCTGCTGTTA AAGCGTGGAG CGAATCCGAA CTATCAGGAC
    ATCTCCGGCT GCACACCTCT GCATCTGGCT GCCAGAAATG GGCAGAAGAA ATGTATGGGC
    AGACTGTTAG AGTACAACGC TGATGTTAAT ATCTGCAATA ATGAAGGCCT CACTGCTATT
    CATTGGCTGG CAGTGAACGG ACGGACAGAA TTACTTCATG ACCTGGTGCA GCACGTCACT
    AATGTAGACG TGGAGGATGC CATGGGACAG ACAGCCTTAC ATGTGGCCTG CCAAAACGGA
    CACAAGACTA CAGTGCAGTG TCTTCTGGAC AGTGGAGCAG ACATCAACAG GCCCAATGTG
    TCTGGAGCCA CTCCACTCTA TTTCGCCTGC AGTCACGGTC AGAGGGACAC GGCACAGATT
    CTCCTCCTCA GGGGAGCCAA GTATCTTCCA GACAGGAATG GAGTTACACC ACTCGACTTG
    TGTGTTCAGG GTGGCTATGG CGAGACGTGT GAAATCCTCA TTCAGCATCA TGGCCGACTG
    TTTCAGACGC TGATACAGAT GACCCAGAAT GATGACATTA AAGAGAACAT GCTGAGGCAG
    GTTCTAGAGC ATGTTTCACA GCAGAATGAC TCCAACTACC AAAGGATCTT GACCAGCTTG
    GCGGAAGTGG CCACTACAAA TGGACACAAA CTGCTGAGTT TGTCCAGTAA CTTTGAAGTC
    CAAACCAAGA GTTTACTGCG GATCATTCGG ATCTTCTGCC ATGTGTTCTG TCTTGGACCG
    TCGTCCCCAA ATAATGGGAA TGATATGGGC TACAATGGAA ATAAGACGCC TCGTAGCCAA
    GTGTTTAAGC CTCTGGAGCT GTTATGGCAC TCTCTGGATG AATGGCTGGT GCTCATCTCC
    ACCGAGCTGG AGAAGGAGAT CACAGACACA ACACGCTCCT CCTCAGGAAA TGACATCGCC
    TCTCTCTTCC TGAAAAAGCA GGAAGTCGAC CACTCAGTTT CCAGTGAAAA CCCTCAGCTT
    CTGCTGGATG CCAGTTCTGT GATGAAGACG CCCGAGGTTT ACGCTGACGG TCAGGATGTC
    ATCTCAATGA TCGCCAATCG GCTGAGCGCT GTCATCCAGG CCTTCTACAT GTGCTGCTCC
    TGTCAGATGC CACATGGCAT GACCTCCCCA CGATTCATCG AGTTTGTCTG CAAGCATGAT
    GAAGTGCTGA AGTGTTTTGT GACAAGGAAT CCCAAAATAA TCTTCAACCA CTTCCATTTC
    CTGCTGGAGT GTCCTGAGCT CATGTCCCGC TTCATGCACA TAATCAAGGG GCAGCCGTTT
    AAAGACCGGT GCGAGTGGTT TTATGAACAT CTTCTTGCTG GACAGCCAGA CTCCGATATG
    GTGCATCGAC CGGTTAATGA AAACGACATC CTTCTCGTGC ACAGAGACTC GTTATTCAGA
    AGCAGCTGTG AAGTCGTGTC CAAGTCCTCC AATGAGAAAC TCAAACAAGG CATCGCCGTG
    CGGTTTCACG GTGAAGAGGG AATGGGTCAG GGAGTGGTCA GAGAGTGGTT CGACATTCTT
    TCCAATGAGA TCATCAACCC AGACTATGCA CTCTTCACAC AATCTGCTGA TGGTACCACG
    TTTCAACCCA ACAGTAACTC CTCTGTAAAC CCAGATCATC TGAACTACTT CCGATTTGCT
    GGTCAAATCC TGGGTCTGGC GCTCTATCAT CGACAGCTGG AATCCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21011
    Clone ID Related Accession (Same CDS sequence) XM_696143.8 , XM_696143.9
    Accession Version XM_696143.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_696143.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGGAAC GGGCGATGGA GCATCTGAAC GTGCAGCTGA ATAGATTGAC GCGCTCTCTG 
    CGTCGCGCCC GGACTGTGGA GCTCCCCGAA GACAGTGAAA CCGCAGTGTA CACCCTCATG
    CCCATGGTCA TGGCTGACCA GCACAGGTCA GTTTCTGAAT TGCTTTTAAA CTCTAAGTTT
    GATGTGAACT ACGCCTTTGG ACGTGTTAAA AGAAGCCTTC TACACATCGC TGCCAATTGT
    GGATCTGTAG AGTGTCTCGT CCTGCTGTTA AAGCGTGGAG CGAATCCGAA CTATCAGGAC
    ATCTCCGGCT GCACACCTCT GCATCTGGCT GCCAGAAATG GGCAGAAGAA ATGTATGGGC
    AGACTGTTAG AGTACAACGC TGATGTTAAT ATCTGCAATA ATGAAGGCCT CACTGCTATT
    CATTGGCTGG CAGTGAACGG ACGGACAGAA TTACTTCATG ACCTGGTGCA GCACGTCACT
    AATGTAGACG TGGAGGATGC CATGGGACAG ACAGCCTTAC ATGTGGCCTG CCAAAACGGA
    CACAAGACTA CAGTGCAGTG TCTTCTGGAC AGTGGAGCAG ACATCAACAG GCCCAATGTG
    TCTGGAGCCA CTCCACTCTA TTTCGCCTGC AGTCACGGTC AGAGGGACAC GGCACAGATT
    CTCCTCCTCA GGGGAGCCAA GTATCTTCCA GACAGGAATG GAGTTACACC ACTCGACTTG
    TGTGTTCAGG GTGGCTATGG CGAGACGTGT GAAATCCTCA TTCAGCATCA TGGCCGACTG
    TTTCAGACGC TGATACAGAT GACCCAGAAT GATGACATTA AAGAGAACAT GCTGAGGCAG
    GTTCTAGAGC ATGTTTCACA GCAGAATGAC TCCAACTACC AAAGGATCTT GACCAGCTTG
    GCGGAAGTGG CCACTACAAA TGGACACAAA CTGCTGAGTT TGTCCAGTAA CTTTGAAGTC
    CAAACCAAGA GTTTACTGCG GATCATTCGG ATCTTCTGCC ATGTGTTCTG TCTTGGACCG
    TCGTCCCCAA ATAATGGGAA TGATATGGGC TACAATGGAA ATAAGACGCC TCGTAGCCAA
    GTGTTTAAGC CTCTGGAGCT GTTATGGCAC TCTCTGGATG AATGGCTGGT GCTCATCTCC
    ACCGAGCTGG AGAAGGAGAT CACAGACACA ACACGCTCCT CCTCAGGAAA TGACATCGCC
    TCTCTCTTCC TGAAAAAGCA GGAAGTCGAC CACTCAGTTT CCAGTGAAAA CCCTCAGCTT
    CTGCTGGATG CCAGTTCTGT GATGAAGACG CCCGAGGTTT ACGCTGACGG TCAGGATGTC
    ATCTCAATGA TCGCCAATCG GCTGAGCGCT GTCATCCAGG CCTTCTACAT GTGCTGCTCC
    TGTCAGATGC CACATGGCAT GACCTCCCCA CGATTCATCG AGTTTGTCTG CAAGCATGAT
    GAAGTGCTGA AGTGTTTTGT GACAAGGAAT CCCAAAATAA TCTTCAACCA CTTCCATTTC
    CTGCTGGAGT GTCCTGAGCT CATGTCCCGC TTCATGCACA TAATCAAGGG GCAGCCGTTT
    AAAGACCGGT GCGAGTGGTT TTATGAACAT CTTCTTGCTG GACAGCCAGA CTCCGATATG
    GTGCATCGAC CGGTTAATGA AAACGACATC CTTCTCGTGC ACAGAGACTC GTTATTCAGA
    AGCAGCTGTG AAGTCGTGTC CAAGTCCTCC AATGAGAAAC TCAAACAAGG CATCGCCGTG
    CGGTTTCACG GTGAAGAGGG AATGGGTCAG GGAGTGGTCA GAGAGTGGTT CGACATTCTT
    TCCAATGAGA TCATCAACCC AGACTATGCA CTCTTCACAC AATCTGCTGA TGGTACCACG
    TTTCAACCCA ACAGTAACTC CTCTGTAAAC CCAGATCATC TGAACTACTT CCGATTTGCT
    GGTCAAATCC TGGGTCTGGC GCTCTATCAT CGACAGCTGG TGAACATCTA CTTCACACGC
    TCCTTTTACA AGCATATTCT GGGAATCCCA GTGAGCTATC AGGACGTGTC TTCCATTGAC
    CCAGAGTATG CAAAAAACCT TCAGTGGATT CTGGACAACG ACATCAGTGA CCTCGGCCTG
    GAGCTCACCT TCTCTGTCGA GACTGATGTG TTCGGTACAA TGGAAGAAGT TCCTCTCAAG
    CCAGGAGGAA CTACCATTCA GGTCACCCAA GACAACAAGG AGGAGTATGT GCAGCTGGTG
    ACGGAGCTCA GGATGACCCG AGCGATTCAG CCGCAGATCA ATGCTTTCCT GCAGGGCTTT
    CACACTTTCA TCCCCCCCTC GCTCATCCAG CTCTTTGATG AATATGAGCT GGAACTGCTG
    TTATCAGGCA TGCCTGAAAT CGATGTGATG GACTGGAAGA GGAACACAGA GTACACCAGT
    GGCTACGACC TGCAGGAGCC TGTTATACAG TGGTTTTGGG AAGTGGTTGA AAATCTCACC
    CAAGAAGAAA GAGTGCTTCT CTTACAGTTT GTGACAGGAA GTTCGAGGGT CCCTCATGGA
    GGCTTTGCTT TCCTCATGGG TGGCAGTGGT CTGCAGAAGT TCACTGTCGC CGCTGTGCCC
    TACACATCAA ACCTACTGCC TACCTCTAGT ACATGCATTA ACATGTTGAA GCTCCCAGAA
    TACCCCAGCA AGGACGTCCT GCGGGATCGC TTGCTTGTCG CACTGCATTG TGGGAGTTAT
    GGATACACCA TGGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_701235.5
    CDS206..2923
    Translation

    Target ORF information:

    RefSeq Version XM_696143.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (hace1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_696143.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGGAAC GGGCGATGGA GCATCTGAAC GTGCAGCTGA ATAGATTGAC GCGCTCTCTG 
    CGTCGCGCCC GGACTGTGGA GCTCCCCGAA GACAGTGAAA CCGCAGTGTA CACCCTCATG
    CCCATGGTCA TGGCTGACCA GCACAGGTCA GTTTCTGAAT TGCTTTTAAA CTCTAAGTTT
    GATGTGAACT ACGCCTTTGG ACGTGTTAAA AGAAGCCTTC TACACATCGC TGCCAATTGT
    GGATCTGTAG AGTGTCTCGT CCTGCTGTTA AAGCGTGGAG CGAATCCGAA CTATCAGGAC
    ATCTCCGGCT GCACACCTCT GCATCTGGCT GCCAGAAATG GGCAGAAGAA ATGTATGGGC
    AGACTGTTAG AGTACAACGC TGATGTTAAT ATCTGCAATA ATGAAGGCCT CACTGCTATT
    CATTGGCTGG CAGTGAACGG ACGGACAGAA TTACTTCATG ACCTGGTGCA GCACGTCACT
    AATGTAGACG TGGAGGATGC CATGGGACAG ACAGCCTTAC ATGTGGCCTG CCAAAACGGA
    CACAAGACTA CAGTGCAGTG TCTTCTGGAC AGTGGAGCAG ACATCAACAG GCCCAATGTG
    TCTGGAGCCA CTCCACTCTA TTTCGCCTGC AGTCACGGTC AGAGGGACAC GGCACAGATT
    CTCCTCCTCA GGGGAGCCAA GTATCTTCCA GACAGGAATG GAGTTACACC ACTCGACTTG
    TGTGTTCAGG GTGGCTATGG CGAGACGTGT GAAATCCTCA TTCAGCATCA TGGCCGACTG
    TTTCAGACGC TGATACAGAT GACCCAGAAT GATGACATTA AAGAGAACAT GCTGAGGCAG
    GTTCTAGAGC ATGTTTCACA GCAGAATGAC TCCAACTACC AAAGGATCTT GACCAGCTTG
    GCGGAAGTGG CCACTACAAA TGGACACAAA CTGCTGAGTT TGTCCAGTAA CTTTGAAGTC
    CAAACCAAGA GTTTACTGCG GATCATTCGG ATCTTCTGCC ATGTGTTCTG TCTTGGACCG
    TCGTCCCCAA ATAATGGGAA TGATATGGGC TACAATGGAA ATAAGACGCC TCGTAGCCAA
    GTGTTTAAGC CTCTGGAGCT GTTATGGCAC TCTCTGGATG AATGGCTGGT GCTCATCTCC
    ACCGAGCTGG AGAAGGAGAT CACAGACACA ACACGCTCCT CCTCAGGAAA TGACATCGCC
    TCTCTCTTCC TGAAAAAGCA GGAAGTCGAC CACTCAGTTT CCAGTGAAAA CCCTCAGCTT
    CTGCTGGATG CCAGTTCTGT GATGAAGACG CCCGAGGTTT ACGCTGACGG TCAGGATGTC
    ATCTCAATGA TCGCCAATCG GCTGAGCGCT GTCATCCAGG CCTTCTACAT GTGCTGCTCC
    TGTCAGATGC CACATGGCAT GACCTCCCCA CGATTCATCG AGTTTGTCTG CAAGCATGAT
    GAAGTGCTGA AGTGTTTTGT GACAAGGAAT CCCAAAATAA TCTTCAACCA CTTCCATTTC
    CTGCTGGAGT GTCCTGAGCT CATGTCCCGC TTCATGCACA TAATCAAGGG GCAGCCGTTT
    AAAGACCGGT GCGAGTGGTT TTATGAACAT CTTCTTGCTG GACAGCCAGA CTCCGATATG
    GTGCATCGAC CGGTTAATGA AAACGACATC CTTCTCGTGC ACAGAGACTC GTTATTCAGA
    AGCAGCTGTG AAGTCGTGTC CAAGTCCTCC AATGAGAAAC TCAAACAAGG CATCGCCGTG
    CGGTTTCACG GTGAAGAGGG AATGGGTCAG GGAGTGGTCA GAGAGTGGTT CGACATTCTT
    TCCAATGAGA TCATCAACCC AGACTATGCA CTCTTCACAC AATCTGCTGA TGGTACCACG
    TTTCAACCCA ACAGTAACTC CTCTGTAAAC CCAGATCATC TGAACTACTT CCGATTTGCT
    GGTCAAATCC TGGGTCTGGC GCTCTATCAT CGACAGCTGG TGAACATCTA CTTCACACGC
    TCCTTTTACA AGCATATTCT GGGAATCCCA GTGAGCTATC AGGACGTGTC TTCCATTGAC
    CCAGAGTATG CAAAAAACCT TCAGTGGATT CTGGACAACG ACATCAGTGA CCTCGGCCTG
    GAGCTCACCT TCTCTGTCGA GACTGATGTG TTCGGTACAA TGGAAGAAGT TCCTCTCAAG
    CCAGGAGGAA CTACCATTCA GGTCACCCAA GACAACAAGG AGGAGTATGT GCAGCTGGTG
    ACGGAGCTCA GGATGACCCG AGCGATTCAG CCGCAGATCA ATGCTTTCCT GCAGGGCTTT
    CACACTTTCA TCCCCCCCTC GCTCATCCAG CTCTTTGATG AATATGAGCT GGAACTGCTG
    TTATCAGGCA TGCCTGAAAT CGATGTGATG GACTGGAAGA GGAACACAGA GTACACCAGT
    GGCTACGACC TGCAGGAGCC TGTTATACAG TGGTTTTGGG AAGTGGTTGA AAATCTCACC
    CAAGAAGAAA GAGTGCTTCT CTTACAGTTT GTGACAGGAA GTTCGAGGGT CCCTCATGGA
    GGCTTTGCTT TCCTCATGGG TGGCAGTGGT CTGCAGAAGT TCACTGTCGC CGCTGTGCCC
    TACACATCAA ACCTACTGCC TACCTCTAGT ACATGCATTA ACATGTTGAA GCTCCCAGAA
    TACCCCAGCA AGGACGTCCT GCGGGATCGC TTGCTTGTCG CACTGCATTG TGGGAGTTAT
    GGATACACCA TGGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21011
    Clone ID Related Accession (Same CDS sequence) XM_696143.8 , XM_696143.9
    Accession Version XM_696143.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HACE1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_696143.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGGAAC GGGCGATGGA GCATCTGAAC GTGCAGCTGA ATAGATTGAC GCGCTCTCTG 
    CGTCGCGCCC GGACTGTGGA GCTCCCCGAA GACAGTGAAA CCGCAGTGTA CACCCTCATG
    CCCATGGTCA TGGCTGACCA GCACAGGTCA GTTTCTGAAT TGCTTTTAAA CTCTAAGTTT
    GATGTGAACT ACGCCTTTGG ACGTGTTAAA AGAAGCCTTC TACACATCGC TGCCAATTGT
    GGATCTGTAG AGTGTCTCGT CCTGCTGTTA AAGCGTGGAG CGAATCCGAA CTATCAGGAC
    ATCTCCGGCT GCACACCTCT GCATCTGGCT GCCAGAAATG GGCAGAAGAA ATGTATGGGC
    AGACTGTTAG AGTACAACGC TGATGTTAAT ATCTGCAATA ATGAAGGCCT CACTGCTATT
    CATTGGCTGG CAGTGAACGG ACGGACAGAA TTACTTCATG ACCTGGTGCA GCACGTCACT
    AATGTAGACG TGGAGGATGC CATGGGACAG ACAGCCTTAC ATGTGGCCTG CCAAAACGGA
    CACAAGACTA CAGTGCAGTG TCTTCTGGAC AGTGGAGCAG ACATCAACAG GCCCAATGTG
    TCTGGAGCCA CTCCACTCTA TTTCGCCTGC AGTCACGGTC AGAGGGACAC GGCACAGATT
    CTCCTCCTCA GGGGAGCCAA GTATCTTCCA GACAGGAATG GAGTTACACC ACTCGACTTG
    TGTGTTCAGG GTGGCTATGG CGAGACGTGT GAAATCCTCA TTCAGCATCA TGGCCGACTG
    TTTCAGACGC TGATACAGAT GACCCAGAAT GATGACATTA AAGAGAACAT GCTGAGGCAG
    GTTCTAGAGC ATGTTTCACA GCAGAATGAC TCCAACTACC AAAGGATCTT GACCAGCTTG
    GCGGAAGTGG CCACTACAAA TGGACACAAA CTGCTGAGTT TGTCCAGTAA CTTTGAAGTC
    CAAACCAAGA GTTTACTGCG GATCATTCGG ATCTTCTGCC ATGTGTTCTG TCTTGGACCG
    TCGTCCCCAA ATAATGGGAA TGATATGGGC TACAATGGAA ATAAGACGCC TCGTAGCCAA
    GTGTTTAAGC CTCTGGAGCT GTTATGGCAC TCTCTGGATG AATGGCTGGT GCTCATCTCC
    ACCGAGCTGG AGAAGGAGAT CACAGACACA ACACGCTCCT CCTCAGGAAA TGACATCGCC
    TCTCTCTTCC TGAAAAAGCA GGAAGTCGAC CACTCAGTTT CCAGTGAAAA CCCTCAGCTT
    CTGCTGGATG CCAGTTCTGT GATGAAGACG CCCGAGGTTT ACGCTGACGG TCAGGATGTC
    ATCTCAATGA TCGCCAATCG GCTGAGCGCT GTCATCCAGG CCTTCTACAT GTGCTGCTCC
    TGTCAGATGC CACATGGCAT GACCTCCCCA CGATTCATCG AGTTTGTCTG CAAGCATGAT
    GAAGTGCTGA AGTGTTTTGT GACAAGGAAT CCCAAAATAA TCTTCAACCA CTTCCATTTC
    CTGCTGGAGT GTCCTGAGCT CATGTCCCGC TTCATGCACA TAATCAAGGG GCAGCCGTTT
    AAAGACCGGT GCGAGTGGTT TTATGAACAT CTTCTTGCTG GACAGCCAGA CTCCGATATG
    GTGCATCGAC CGGTTAATGA AAACGACATC CTTCTCGTGC ACAGAGACTC GTTATTCAGA
    AGCAGCTGTG AAGTCGTGTC CAAGTCCTCC AATGAGAAAC TCAAACAAGG CATCGCCGTG
    CGGTTTCACG GTGAAGAGGG AATGGGTCAG GGAGTGGTCA GAGAGTGGTT CGACATTCTT
    TCCAATGAGA TCATCAACCC AGACTATGCA CTCTTCACAC AATCTGCTGA TGGTACCACG
    TTTCAACCCA ACAGTAACTC CTCTGTAAAC CCAGATCATC TGAACTACTT CCGATTTGCT
    GGTCAAATCC TGGGTCTGGC GCTCTATCAT CGACAGCTGG TGAACATCTA CTTCACACGC
    TCCTTTTACA AGCATATTCT GGGAATCCCA GTGAGCTATC AGGACGTGTC TTCCATTGAC
    CCAGAGTATG CAAAAAACCT TCAGTGGATT CTGGACAACG ACATCAGTGA CCTCGGCCTG
    GAGCTCACCT TCTCTGTCGA GACTGATGTG TTCGGTACAA TGGAAGAAGT TCCTCTCAAG
    CCAGGAGGAA CTACCATTCA GGTCACCCAA GACAACAAGG AGGAGTATGT GCAGCTGGTG
    ACGGAGCTCA GGATGACCCG AGCGATTCAG CCGCAGATCA ATGCTTTCCT GCAGGGCTTT
    CACACTTTCA TCCCCCCCTC GCTCATCCAG CTCTTTGATG AATATGAGCT GGAACTGCTG
    TTATCAGGCA TGCCTGAAAT CGATGTGATG GACTGGAAGA GGAACACAGA GTACACCAGT
    GGCTACGACC TGCAGGAGCC TGTTATACAG TGGTTTTGGG AAGTGGTTGA AAATCTCACC
    CAAGAAGAAA GAGTGCTTCT CTTACAGTTT GTGACAGGAA GTTCGAGGGT CCCTCATGGA
    GGCTTTGCTT TCCTCATGGG TGGCAGTGGT CTGCAGAAGT TCACTGTCGC CGCTGTGCCC
    TACACATCAA ACCTACTGCC TACCTCTAGT ACATGCATTA ACATGTTGAA GCTCCCAGAA
    TACCCCAGCA AGGACGTCCT GCGGGATCGC TTGCTTGTCG CACTGCATTG TGGGAGTTAT
    GGATACACCA TGGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_701235.5
    CDS209..2926
    Translation

    Target ORF information:

    RefSeq Version XM_696143.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (hace1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_696143.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGGAAC GGGCGATGGA GCATCTGAAC GTGCAGCTGA ATAGATTGAC GCGCTCTCTG 
    CGTCGCGCCC GGACTGTGGA GCTCCCCGAA GACAGTGAAA CCGCAGTGTA CACCCTCATG
    CCCATGGTCA TGGCTGACCA GCACAGGTCA GTTTCTGAAT TGCTTTTAAA CTCTAAGTTT
    GATGTGAACT ACGCCTTTGG ACGTGTTAAA AGAAGCCTTC TACACATCGC TGCCAATTGT
    GGATCTGTAG AGTGTCTCGT CCTGCTGTTA AAGCGTGGAG CGAATCCGAA CTATCAGGAC
    ATCTCCGGCT GCACACCTCT GCATCTGGCT GCCAGAAATG GGCAGAAGAA ATGTATGGGC
    AGACTGTTAG AGTACAACGC TGATGTTAAT ATCTGCAATA ATGAAGGCCT CACTGCTATT
    CATTGGCTGG CAGTGAACGG ACGGACAGAA TTACTTCATG ACCTGGTGCA GCACGTCACT
    AATGTAGACG TGGAGGATGC CATGGGACAG ACAGCCTTAC ATGTGGCCTG CCAAAACGGA
    CACAAGACTA CAGTGCAGTG TCTTCTGGAC AGTGGAGCAG ACATCAACAG GCCCAATGTG
    TCTGGAGCCA CTCCACTCTA TTTCGCCTGC AGTCACGGTC AGAGGGACAC GGCACAGATT
    CTCCTCCTCA GGGGAGCCAA GTATCTTCCA GACAGGAATG GAGTTACACC ACTCGACTTG
    TGTGTTCAGG GTGGCTATGG CGAGACGTGT GAAATCCTCA TTCAGCATCA TGGCCGACTG
    TTTCAGACGC TGATACAGAT GACCCAGAAT GATGACATTA AAGAGAACAT GCTGAGGCAG
    GTTCTAGAGC ATGTTTCACA GCAGAATGAC TCCAACTACC AAAGGATCTT GACCAGCTTG
    GCGGAAGTGG CCACTACAAA TGGACACAAA CTGCTGAGTT TGTCCAGTAA CTTTGAAGTC
    CAAACCAAGA GTTTACTGCG GATCATTCGG ATCTTCTGCC ATGTGTTCTG TCTTGGACCG
    TCGTCCCCAA ATAATGGGAA TGATATGGGC TACAATGGAA ATAAGACGCC TCGTAGCCAA
    GTGTTTAAGC CTCTGGAGCT GTTATGGCAC TCTCTGGATG AATGGCTGGT GCTCATCTCC
    ACCGAGCTGG AGAAGGAGAT CACAGACACA ACACGCTCCT CCTCAGGAAA TGACATCGCC
    TCTCTCTTCC TGAAAAAGCA GGAAGTCGAC CACTCAGTTT CCAGTGAAAA CCCTCAGCTT
    CTGCTGGATG CCAGTTCTGT GATGAAGACG CCCGAGGTTT ACGCTGACGG TCAGGATGTC
    ATCTCAATGA TCGCCAATCG GCTGAGCGCT GTCATCCAGG CCTTCTACAT GTGCTGCTCC
    TGTCAGATGC CACATGGCAT GACCTCCCCA CGATTCATCG AGTTTGTCTG CAAGCATGAT
    GAAGTGCTGA AGTGTTTTGT GACAAGGAAT CCCAAAATAA TCTTCAACCA CTTCCATTTC
    CTGCTGGAGT GTCCTGAGCT CATGTCCCGC TTCATGCACA TAATCAAGGG GCAGCCGTTT
    AAAGACCGGT GCGAGTGGTT TTATGAACAT CTTCTTGCTG GACAGCCAGA CTCCGATATG
    GTGCATCGAC CGGTTAATGA AAACGACATC CTTCTCGTGC ACAGAGACTC GTTATTCAGA
    AGCAGCTGTG AAGTCGTGTC CAAGTCCTCC AATGAGAAAC TCAAACAAGG CATCGCCGTG
    CGGTTTCACG GTGAAGAGGG AATGGGTCAG GGAGTGGTCA GAGAGTGGTT CGACATTCTT
    TCCAATGAGA TCATCAACCC AGACTATGCA CTCTTCACAC AATCTGCTGA TGGTACCACG
    TTTCAACCCA ACAGTAACTC CTCTGTAAAC CCAGATCATC TGAACTACTT CCGATTTGCT
    GGTCAAATCC TGGGTCTGGC GCTCTATCAT CGACAGCTGG TGAACATCTA CTTCACACGC
    TCCTTTTACA AGCATATTCT GGGAATCCCA GTGAGCTATC AGGACGTGTC TTCCATTGAC
    CCAGAGTATG CAAAAAACCT TCAGTGGATT CTGGACAACG ACATCAGTGA CCTCGGCCTG
    GAGCTCACCT TCTCTGTCGA GACTGATGTG TTCGGTACAA TGGAAGAAGT TCCTCTCAAG
    CCAGGAGGAA CTACCATTCA GGTCACCCAA GACAACAAGG AGGAGTATGT GCAGCTGGTG
    ACGGAGCTCA GGATGACCCG AGCGATTCAG CCGCAGATCA ATGCTTTCCT GCAGGGCTTT
    CACACTTTCA TCCCCCCCTC GCTCATCCAG CTCTTTGATG AATATGAGCT GGAACTGCTG
    TTATCAGGCA TGCCTGAAAT CGATGTGATG GACTGGAAGA GGAACACAGA GTACACCAGT
    GGCTACGACC TGCAGGAGCC TGTTATACAG TGGTTTTGGG AAGTGGTTGA AAATCTCACC
    CAAGAAGAAA GAGTGCTTCT CTTACAGTTT GTGACAGGAA GTTCGAGGGT CCCTCATGGA
    GGCTTTGCTT TCCTCATGGG TGGCAGTGGT CTGCAGAAGT TCACTGTCGC CGCTGTGCCC
    TACACATCAA ACCTACTGCC TACCTCTAGT ACATGCATTA ACATGTTGAA GCTCCCAGAA
    TACCCCAGCA AGGACGTCCT GCGGGATCGC TTGCTTGTCG CACTGCATTG TGGGAGTTAT
    GGATACACCA TGGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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