robo2 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa148975 XM_021481452.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148976 XM_021481453.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148977 XM_021481454.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148978 XM_021481455.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148979 XM_021481456.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148980 XM_021481457.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148981 XM_021481458.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148982 XM_021481459.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148983 XM_021481460.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148984 XM_021481461.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55117 XM_017350998.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55118 XM_017350999.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55119 XM_017351000.2
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55120 XM_017351001.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55121 XM_017351002.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55122 XM_017351003.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55123 XM_017351004.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55124 XM_017351006.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55125 XM_017351007.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55126 XM_017351008.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55127 XM_017351009.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55128 XM_017351010.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa02796 NM_131633.1
Latest version!
Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa55119 XM_017351000.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa148975
    Clone ID Related Accession (Same CDS sequence) XM_021481452.1
    Accession Version XM_021481452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT
    TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC
    GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT
    GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT
    CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC
    CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT
    GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC
    ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG
    CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA
    TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC
    AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA
    TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG
    GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA
    GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337127.1
    CDS252..5039
    Translation

    Target ORF information:

    RefSeq Version XM_021481452.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT
    TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC
    GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT
    GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT
    CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC
    CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT
    GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC
    ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG
    CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA
    TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC
    AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA
    TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG
    GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA
    GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148976
    Clone ID Related Accession (Same CDS sequence) XM_021481453.1
    Accession Version XM_021481453.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337128.1
    CDS252..5027
    Translation

    Target ORF information:

    RefSeq Version XM_021481453.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148977
    Clone ID Related Accession (Same CDS sequence) XM_021481454.1
    Accession Version XM_021481454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG
    CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT
    GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC
    AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC
    GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT
    GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA
    GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG
    CCCGACAAAA ACTCCTGCAA TAACACACTC CTTTTCATTC CTGACTACCG ATTGGCTGAC
    GGACTGTCTA ATAGAATGCC ACACAACCAA TCGCAGGATT TCAGCACCAC CAGCTCCCAC
    AACAGCTCAG ATCGGAGCAA CAGTCTGTCA GGTGGAAAAG GTGGTAAGAA GAAGAAGACC
    AAAGCAAACG TCAAGCCCAC CAAGATGAAT GGCTCCAACT GGGCGAACGT TCCACTTCCT
    CCTCCTCCCA TCCACCCTCT TCCTGGCACA GAGATGGACC ACTACGCCTC AGAGCACCAT
    GATGGAGGAG GATATGATAG TGATGGTTGG GGCCCACCAT TGCCAGTGCA GACGTACCTC
    CATCAGGGTT TAGAGGATGA GCTGGAGGAA GAGGAAGAGC GGGTGCCAAC TCCTCCCATT
    AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT TCCTTTGGCA AACAGTCCAC AGCCACACTG
    AGCCCATCTC CACGTGAAGA AATGCAGCCC ATGCTCCAAG CCCACCTAGA TGAGCTTACC
    CGTGCTTATC AATTGGACAT GGCTAAACAG ACTTGGCACA TGCAGGGTGG GCCTTACCCG
    CCCCAGGCCC CCGCACCACC CGTGGGCTAC GTCTCCAGCA CCTTAGTGTC TGACATCGAG
    ACCGATCTCC CTGATGAAGA TGACGATGAT GAGGAGGATG AAGGATACGA GATGTCGCCA
    CCAATTAGGG GCACTGAACA CACGCCAGGC TCCAGCATGG ACAACTTAGA CAGCTCCGTT
    ACGGGCTCTA TGGTGAACGG CTGGGGCTCG GCTTCAGAAG ATGATGACAG AAACTTCTCC
    AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT GACGGCTCGC TCTTCACCCA TTGCAGCTTT
    GCGCGAGCCC TGGTGGCAGC CGCAGACAAG GCTGGTTACC GTCTGGATGG CACCAGCCTG
    AGCAAAAGAG ATAAAGGTCT ACCTCATAGA CCTCGTCCCG CTAGCCCGTT CTCCACAGAT
    GGAAGCACGG TGGGCACACA CAGTCTGGGG CACCAGCGGG CAGCAAGGCC CACACGTAAA
    CACAAAGGCC CTGGCACTGT CCCGAACCGC CGTGATGCCA GTGCGGATGA TCTGCCTCCA
    CCTCCAGAGC CTCCACCCTG CCAGACAGCT CGCATCCCCG GGTCGAGGAA CGTTCCCCAT
    CCCTGCGACC GCAAAGCTTC CTCTCTGGAG AGGCAGCCTA TGATGGGCCT TGAGGAAATG
    AGAAGTTCCT TGGACCGGCA GGCCCGAACC TCATTGGACC GCCACCAACA AGTCCAGGAC
    AGACTGGGGT CCATAGAGAG AGGAGATGAA AGCAGAAGAG GACAGAAAAA TGGTCCCTTT
    TCAGAAGAGG CCCTATTGCC ATACAACAAA CCATCATTCC CATCCCCTGG TGGGCACAGC
    TCATCTGGGA CGGCCTCTTC TAAAGGGTCA ACGGGTCCTC GCAAAGGAGA AGGGACGAGG
    GGACAGCACC AGCGCTCCAC CACGGAACAA GGAGAAAGCA GCTACATGGG GACCAACGCG
    CAGTTTTCCG GAGAGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337129.1
    CDS252..5012
    Translation

    Target ORF information:

    RefSeq Version XM_021481454.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG
    CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT
    GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC
    AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC
    GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT
    GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA
    GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG
    CCCGACAAAA ACTCCTGCAA TAACACACTC CTTTTCATTC CTGACTACCG ATTGGCTGAC
    GGACTGTCTA ATAGAATGCC ACACAACCAA TCGCAGGATT TCAGCACCAC CAGCTCCCAC
    AACAGCTCAG ATCGGAGCAA CAGTCTGTCA GGTGGAAAAG GTGGTAAGAA GAAGAAGACC
    AAAGCAAACG TCAAGCCCAC CAAGATGAAT GGCTCCAACT GGGCGAACGT TCCACTTCCT
    CCTCCTCCCA TCCACCCTCT TCCTGGCACA GAGATGGACC ACTACGCCTC AGAGCACCAT
    GATGGAGGAG GATATGATAG TGATGGTTGG GGCCCACCAT TGCCAGTGCA GACGTACCTC
    CATCAGGGTT TAGAGGATGA GCTGGAGGAA GAGGAAGAGC GGGTGCCAAC TCCTCCCATT
    AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT TCCTTTGGCA AACAGTCCAC AGCCACACTG
    AGCCCATCTC CACGTGAAGA AATGCAGCCC ATGCTCCAAG CCCACCTAGA TGAGCTTACC
    CGTGCTTATC AATTGGACAT GGCTAAACAG ACTTGGCACA TGCAGGGTGG GCCTTACCCG
    CCCCAGGCCC CCGCACCACC CGTGGGCTAC GTCTCCAGCA CCTTAGTGTC TGACATCGAG
    ACCGATCTCC CTGATGAAGA TGACGATGAT GAGGAGGATG AAGGATACGA GATGTCGCCA
    CCAATTAGGG GCACTGAACA CACGCCAGGC TCCAGCATGG ACAACTTAGA CAGCTCCGTT
    ACGGGCTCTA TGGTGAACGG CTGGGGCTCG GCTTCAGAAG ATGATGACAG AAACTTCTCC
    AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT GACGGCTCGC TCTTCACCCA TTGCAGCTTT
    GCGCGAGCCC TGGTGGCAGC CGCAGACAAG GCTGGTTACC GTCTGGATGG CACCAGCCTG
    AGCAAAAGAG ATAAAGGTCT ACCTCATAGA CCTCGTCCCG CTAGCCCGTT CTCCACAGAT
    GGAAGCACGG TGGGCACACA CAGTCTGGGG CACCAGCGGG CAGCAAGGCC CACACGTAAA
    CACAAAGGCC CTGGCACTGT CCCGAACCGC CGTGATGCCA GTGCGGATGA TCTGCCTCCA
    CCTCCAGAGC CTCCACCCTG CCAGACAGCT CGCATCCCCG GGTCGAGGAA CGTTCCCCAT
    CCCTGCGACC GCAAAGCTTC CTCTCTGGAG AGGCAGCCTA TGATGGGCCT TGAGGAAATG
    AGAAGTTCCT TGGACCGGCA GGCCCGAACC TCATTGGACC GCCACCAACA AGTCCAGGAC
    AGACTGGGGT CCATAGAGAG AGGAGATGAA AGCAGAAGAG GACAGAAAAA TGGTCCCTTT
    TCAGAAGAGG CCCTATTGCC ATACAACAAA CCATCATTCC CATCCCCTGG TGGGCACAGC
    TCATCTGGGA CGGCCTCTTC TAAAGGGTCA ACGGGTCCTC GCAAAGGAGA AGGGACGAGG
    GGACAGCACC AGCGCTCCAC CACGGAACAA GGAGAAAGCA GCTACATGGG GACCAACGCG
    CAGTTTTCCG GAGAGTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148978
    Clone ID Related Accession (Same CDS sequence) XM_021481455.1
    Accession Version XM_021481455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT
    TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC
    GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT
    GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT
    CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC
    CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT
    GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC
    ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG
    CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA
    TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC
    AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA
    TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG
    GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA
    GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337130.1
    CDS618..5345
    Translation

    Target ORF information:

    RefSeq Version XM_021481455.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT
    TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC
    GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT
    GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT
    CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC
    CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT
    GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC
    ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG
    CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA
    TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC
    AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA
    TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG
    GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA
    GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148979
    Clone ID Related Accession (Same CDS sequence) XM_021481456.1
    Accession Version XM_021481456.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337131.1
    CDS618..5333
    Translation

    Target ORF information:

    RefSeq Version XM_021481456.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148980
    Clone ID Related Accession (Same CDS sequence) XM_021481457.1
    Accession Version XM_021481457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CAGTGGAAAA
    GGTGGTAAGA AGAAGAAGAC CAAAGCAAAC GTCAAGCCCA CCAAGATGAA TGGCTCCAAC
    TGGGCGAACG TTCCACTTCC TCCTCCTCCC ATCCACCCTC TTCCTGGCAC AGAGATGGAC
    CACTACGCCT CAGAGCACCA TGATGGAGGA GGATATGATA GTGATGGTTG GGGCCCACCA
    TTGCCAGTGC AGACGTACCT CCATCAGGGT TTAGAGGATG AGCTGGAGGA AGAGGAAGAG
    CGGGTGCCAA CTCCTCCCAT TAGGGGTGTG GCTTCTTCTC CTGCTGCAGT TTCCTTTGGC
    AAACAGTCCA CAGCCACACT GAGCCCATCT CCACGTGAAG AAATGCAGCC CATGCTCCAA
    GCCCACCTAG ATGAGCTTAC CCGTGCTTAT CAATTGGACA TGGCTAAACA GACTTGGCAC
    ATGCAGGGTG GGCCTTACCC GCCCCAGGCC CCCGCACCAC CCGTGGGCTA CGTCTCCAGC
    ACCTTAGTGT CTGACATCGA GACCGATCTC CCTGATGAAG ATGACGATGA TGAGGAGGAT
    GAAGGATACG AGATGTCGCC ACCAATTAGG GGCACTGAAC ACACGCCAGG CTCCAGCATG
    GACAACTTAG ACAGCTCCGT TACGGGCTCT ATGGTGAACG GCTGGGGCTC GGCTTCAGAA
    GATGATGACA GAAACTTCTC CAGCAGGAGG TCTAGTGCCG GCAGCTCTTC TGACGGCTCG
    CTCTTCACCC ATTGCAGCTT TGCGCGAGCC CTGGTGGCAG CCGCAGACAA GGCTGGTTAC
    CGTCTGGATG GCACCAGCCT GAGCAAAAGA GATAAAGGTC TACCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337132.1
    CDS252..4913
    Translation

    Target ORF information:

    RefSeq Version XM_021481457.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CAGTGGAAAA
    GGTGGTAAGA AGAAGAAGAC CAAAGCAAAC GTCAAGCCCA CCAAGATGAA TGGCTCCAAC
    TGGGCGAACG TTCCACTTCC TCCTCCTCCC ATCCACCCTC TTCCTGGCAC AGAGATGGAC
    CACTACGCCT CAGAGCACCA TGATGGAGGA GGATATGATA GTGATGGTTG GGGCCCACCA
    TTGCCAGTGC AGACGTACCT CCATCAGGGT TTAGAGGATG AGCTGGAGGA AGAGGAAGAG
    CGGGTGCCAA CTCCTCCCAT TAGGGGTGTG GCTTCTTCTC CTGCTGCAGT TTCCTTTGGC
    AAACAGTCCA CAGCCACACT GAGCCCATCT CCACGTGAAG AAATGCAGCC CATGCTCCAA
    GCCCACCTAG ATGAGCTTAC CCGTGCTTAT CAATTGGACA TGGCTAAACA GACTTGGCAC
    ATGCAGGGTG GGCCTTACCC GCCCCAGGCC CCCGCACCAC CCGTGGGCTA CGTCTCCAGC
    ACCTTAGTGT CTGACATCGA GACCGATCTC CCTGATGAAG ATGACGATGA TGAGGAGGAT
    GAAGGATACG AGATGTCGCC ACCAATTAGG GGCACTGAAC ACACGCCAGG CTCCAGCATG
    GACAACTTAG ACAGCTCCGT TACGGGCTCT ATGGTGAACG GCTGGGGCTC GGCTTCAGAA
    GATGATGACA GAAACTTCTC CAGCAGGAGG TCTAGTGCCG GCAGCTCTTC TGACGGCTCG
    CTCTTCACCC ATTGCAGCTT TGCGCGAGCC CTGGTGGCAG CCGCAGACAA GGCTGGTTAC
    CGTCTGGATG GCACCAGCCT GAGCAAAAGA GATAAAGGTC TACCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148981
    Clone ID Related Accession (Same CDS sequence) XM_021481458.1
    Accession Version XM_021481458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG
    CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT
    GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC
    AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC
    GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT
    GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA
    GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG
    CCCGACAAAA ACTCCTGCAA TAACAGTGGA AAAGGTGGTA AGAAGAAGAA GACCAAAGCA
    AACGTCAAGC CCACCAAGAT GAATGGCTCC AACTGGGCGA ACGTTCCACT TCCTCCTCCT
    CCCATCCACC CTCTTCCTGG CACAGAGATG GACCACTACG CCTCAGAGCA CCATGATGGA
    GGAGGATATG ATAGTGATGG TTGGGGCCCA CCATTGCCAG TGCAGACGTA CCTCCATCAG
    GGTTTAGAGG ATGAGCTGGA GGAAGAGGAA GAGCGGGTGC CAACTCCTCC CATTAGGGGT
    GTGGCTTCTT CTCCTGCTGC AGTTTCCTTT GGCAAACAGT CCACAGCCAC ACTGAGCCCA
    TCTCCACGTG AAGAAATGCA GCCCATGCTC CAAGCCCACC TAGATGAGCT TACCCGTGCT
    TATCAATTGG ACATGGCTAA ACAGACTTGG CACATGCAGG GTGGGCCTTA CCCGCCCCAG
    GCCCCCGCAC CACCCGTGGG CTACGTCTCC AGCACCTTAG TGTCTGACAT CGAGACCGAT
    CTCCCTGATG AAGATGACGA TGATGAGGAG GATGAAGGAT ACGAGATGTC GCCACCAATT
    AGGGGCACTG AACACACGCC AGGCTCCAGC ATGGACAACT TAGACAGCTC CGTTACGGGC
    TCTATGGTGA ACGGCTGGGG CTCGGCTTCA GAAGATGATG ACAGAAACTT CTCCAGCAGG
    AGGTCTAGTG CCGGCAGCTC TTCTGACGGC TCGCTCTTCA CCCATTGCAG CTTTGCGCGA
    GCCCTGGTGG CAGCCGCAGA CAAGGCTGGT TACCGTCTGG ATGGCACCAG CCTGAGCAAA
    AGAGATAAAG GTCTACCTCA TAGACCTCGT CCCGCTAGCC CGTTCTCCAC AGATGGAAGC
    ACGGTGGGCA CACACAGTCT GGGGCACCAG CGGGCAGCAA GGCCCACACG TAAACACAAA
    GGCCCTGGCA CTGTCCCGAA CCGCCGTGAT GCCAGTGCGG ATGATCTGCC TCCACCTCCA
    GAGCCTCCAC CCTGCCAGAC AGCTCGCATC CCCGGGTCGA GGAACGTTCC CCATCCCTGC
    GACCGCAAAG CTTCCTCTCT GGAGAGGCAG CCTATGATGG GCCTTGAGGA AATGAGAAGT
    TCCTTGGACC GGCAGGCCCG AACCTCATTG GACCGCCACC AACAAGTCCA GGACAGACTG
    GGGTCCATAG AGAGAGGAGA TGAAAGCAGA AGAGGACAGA AAAATGGTCC CTTTTCAGAA
    GAGGCCCTAT TGCCATACAA CAAACCATCA TTCCCATCCC CTGGTGGGCA CAGCTCATCT
    GGGACGGCCT CTTCTAAAGG GTCAACGGGT CCTCGCAAAG GAGAAGGGAC GAGGGGACAG
    CACCAGCGCT CCACCACGGA ACAAGGAGAA AGCAGCTACA TGGGGACCAA CGCGCAGTTT
    TCCGGAGAGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337133.1
    CDS252..4886
    Translation

    Target ORF information:

    RefSeq Version XM_021481458.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG
    CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT
    GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC
    AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC
    GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT
    GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA
    GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG
    CCCGACAAAA ACTCCTGCAA TAACAGTGGA AAAGGTGGTA AGAAGAAGAA GACCAAAGCA
    AACGTCAAGC CCACCAAGAT GAATGGCTCC AACTGGGCGA ACGTTCCACT TCCTCCTCCT
    CCCATCCACC CTCTTCCTGG CACAGAGATG GACCACTACG CCTCAGAGCA CCATGATGGA
    GGAGGATATG ATAGTGATGG TTGGGGCCCA CCATTGCCAG TGCAGACGTA CCTCCATCAG
    GGTTTAGAGG ATGAGCTGGA GGAAGAGGAA GAGCGGGTGC CAACTCCTCC CATTAGGGGT
    GTGGCTTCTT CTCCTGCTGC AGTTTCCTTT GGCAAACAGT CCACAGCCAC ACTGAGCCCA
    TCTCCACGTG AAGAAATGCA GCCCATGCTC CAAGCCCACC TAGATGAGCT TACCCGTGCT
    TATCAATTGG ACATGGCTAA ACAGACTTGG CACATGCAGG GTGGGCCTTA CCCGCCCCAG
    GCCCCCGCAC CACCCGTGGG CTACGTCTCC AGCACCTTAG TGTCTGACAT CGAGACCGAT
    CTCCCTGATG AAGATGACGA TGATGAGGAG GATGAAGGAT ACGAGATGTC GCCACCAATT
    AGGGGCACTG AACACACGCC AGGCTCCAGC ATGGACAACT TAGACAGCTC CGTTACGGGC
    TCTATGGTGA ACGGCTGGGG CTCGGCTTCA GAAGATGATG ACAGAAACTT CTCCAGCAGG
    AGGTCTAGTG CCGGCAGCTC TTCTGACGGC TCGCTCTTCA CCCATTGCAG CTTTGCGCGA
    GCCCTGGTGG CAGCCGCAGA CAAGGCTGGT TACCGTCTGG ATGGCACCAG CCTGAGCAAA
    AGAGATAAAG GTCTACCTCA TAGACCTCGT CCCGCTAGCC CGTTCTCCAC AGATGGAAGC
    ACGGTGGGCA CACACAGTCT GGGGCACCAG CGGGCAGCAA GGCCCACACG TAAACACAAA
    GGCCCTGGCA CTGTCCCGAA CCGCCGTGAT GCCAGTGCGG ATGATCTGCC TCCACCTCCA
    GAGCCTCCAC CCTGCCAGAC AGCTCGCATC CCCGGGTCGA GGAACGTTCC CCATCCCTGC
    GACCGCAAAG CTTCCTCTCT GGAGAGGCAG CCTATGATGG GCCTTGAGGA AATGAGAAGT
    TCCTTGGACC GGCAGGCCCG AACCTCATTG GACCGCCACC AACAAGTCCA GGACAGACTG
    GGGTCCATAG AGAGAGGAGA TGAAAGCAGA AGAGGACAGA AAAATGGTCC CTTTTCAGAA
    GAGGCCCTAT TGCCATACAA CAAACCATCA TTCCCATCCC CTGGTGGGCA CAGCTCATCT
    GGGACGGCCT CTTCTAAAGG GTCAACGGGT CCTCGCAAAG GAGAAGGGAC GAGGGGACAG
    CACCAGCGCT CCACCACGGA ACAAGGAGAA AGCAGCTACA TGGGGACCAA CGCGCAGTTT
    TCCGGAGAGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148982
    Clone ID Related Accession (Same CDS sequence) XM_021481459.1
    Accession Version XM_021481459.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337134.1
    CDS252..4853
    Translation

    Target ORF information:

    RefSeq Version XM_021481459.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481459.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148983
    Clone ID Related Accession (Same CDS sequence) XM_021481460.1
    Accession Version XM_021481460.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4749bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT
    AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT
    CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC
    AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC
    CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA
    TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA
    GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT
    TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA
    CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA
    TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC
    GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT
    GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC
    ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG
    GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT
    AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG
    GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT
    AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG
    GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT
    GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT
    CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC
    AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG
    GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC
    ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC
    CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG
    GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG
    CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA
    GAGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337135.1
    CDS252..5000
    Translation

    Target ORF information:

    RefSeq Version XM_021481460.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT
    AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT
    CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC
    AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC
    CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA
    TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA
    GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT
    TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA
    CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA
    TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC
    GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT
    GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC
    ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG
    GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT
    AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG
    GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT
    AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG
    GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT
    GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT
    CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC
    AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG
    GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC
    ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC
    CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG
    GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG
    CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA
    GAGTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148984
    Clone ID Related Accession (Same CDS sequence) XM_021481461.1
    Accession Version XM_021481461.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC
    ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT
    CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT
    AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT
    GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT
    CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA
    GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC
    ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA
    CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA
    GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA
    CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC
    GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC
    GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA
    GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT
    CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA
    CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT
    GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC
    TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT
    TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG
    CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG
    AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG
    CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT
    TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC
    ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337136.1
    CDS252..4874
    Translation

    Target ORF information:

    RefSeq Version XM_021481461.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481461.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
    CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC
    ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT
    CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT
    AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT
    GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT
    CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA
    GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC
    ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA
    CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA
    GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA
    CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC
    GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC
    GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA
    GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT
    CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA
    CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT
    GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC
    TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT
    TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG
    CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG
    AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG
    CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT
    TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC
    ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55117
    Clone ID Related Accession (Same CDS sequence) XM_017350998.1
    Accession Version XM_017350998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206487.1
    CDS680..5479
    Translation

    Target ORF information:

    RefSeq Version XM_017350998.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017350998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55118
    Clone ID Related Accession (Same CDS sequence) XM_017350999.1
    Accession Version XM_017350999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT
    CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG
    CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG
    CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG
    CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC
    AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG
    GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC
    GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC
    CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT
    TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC
    TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT
    CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC
    TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC
    CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC
    ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA
    GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA
    GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA
    CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG
    TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA
    GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT
    CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC
    ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG
    CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC
    CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG
    AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT
    GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG
    GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG
    ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC
    ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC
    TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TTCAGTCGTT CACCTTCACC
    CCTGCAGTGA CGTTCCAGCG TGTTGATGGA GGCTTGATGA GCAACGGGAG TCGTCCTGGT
    CTGCTGAACG CCAGTGATTC GGGTTACCCG TGGCTCGCCG ACTCCTGGCC TCCCAGCCTG
    CCTGTCAACA GCGGATCTGG AGGACCCAAT GACCTCAGCT TTGGCCGTGG AGATGTGATG
    CCCAGTGCTC AGGCTGATAA GACGGGTACC ATGCTTTCGG ATGGAGCCAT TTACAGCAGC
    ATCGACTTCA CCACCAAAGG CGGATTCGGT AGCCCAAGCC CAGCCTCCCA GGCCACGCCC
    TATGCCACCA CACAGATCCT CCACTCCAAC AGCATCCATG AGCTGGCAGT GGATCTTCCT
    GAAGCCCAGT GGAAGACCTC CCTACAGGCC AAGCAGGAGA TGGCCAGCCT GGGTTACTCA
    CTGCCCGACA AAAACTCCTG CAATAACACA CTCCTTTTCA TTCCTGACTA CCGATTGGCT
    GACGGACTGT CTAATAGAAT GCCACACAAC CAATCGCAGG ATTTCAGCAC CACCAGCTCC
    CACAACAGCT CAGATCGGAG CAACAGTCTG TCAGGTGGAA AAGGTGGTAA GAAGAAGAAG
    ACCAAAGCAA ACGTCAAGCC CACCAAGATG AATGGCTCCA ACTGGGCGAA CGTTCCACTT
    CCTCCTCCTC CCATCCACCC TCTTCCTGGC ACAGAGATGG ACCACTACGC CTCAGAGCAC
    CATGATGGAG GAGGATATGA TAGTGATGGT TGGGGCCCAC CATTGCCAGT GCAGACGTAC
    CTCCATCAGG GTTTAGAGGA TGAGCTGGAG GAAGAGGAAG AGCGGGTGCC AACTCCTCCC
    ATTAGGGGTG TGGCTTCTTC TCCTGCTGCA GTTTCCTTTG GCAAACAGTC CACAGCCACA
    CTGAGCCCAT CTCCACGTGA AGAAATGCAG CCCATGCTCC AAGCCCACCT AGATGAGCTT
    ACCCGTGCTT ATCAATTGGA CATGGCTAAA CAGACTTGGC ACATGCAGGG TGGGCCTTAC
    CCGCCCCAGG CCCCCGCACC ACCCGTGGGC TACGTCTCCA GCACCTTAGT GTCTGACATC
    GAGACCGATC TCCCTGATGA AGATGACGAT GATGAGGAGG ATGAAGGATA CGAGATGTCG
    CCACCAATTA GGGGCACTGA ACACACGCCA GGCTCCAGCA TGGACAACTT AGACAGCTCC
    GTTACGGGCT CTATGGTGAA CGGCTGGGGC TCGGCTTCAG AAGATGATGA CAGAAACTTC
    TCCAGCAGGA GGTCTAGTGC CGGCAGCTCT TCTGACGGCT CGCTCTTCAC CCATTGCAGC
    TTTGCGCGAG CCCTGGTGGC AGCCGCAGAC AAGGCTGGTT ACCGTCTGGA TGGCACCAGC
    CTGAGCAAAA GAGATAAAGG TCTACCTCAT AGACCTCGTC CCGCTAGCCC GTTCTCCACA
    GATGGAAGCA CGGTGGGCAC ACACAGTCTG GGGCACCAGC GGGCAGCAAG GCCCACACGT
    AAACACAAAG GCCCTGGCAC TGTCCCGAAC CGCCGTGATG CCAGTGCGGA TGATCTGCCT
    CCACCTCCAG AGCCTCCACC CTGCCAGACA GCTCGCATCC CCGGGTCGAG GAACGTTCCC
    CATCCCTGCG ACCGCAAAGC TTCCTCTCTG GAGAGGCAGC CTATGATGGG CCTTGAGGAA
    ATGAGAAGTT CCTTGGACCG GCAGGCCCGA ACCTCATTGG ACCGCCACCA ACAAGTCCAG
    GACAGACTGG GGTCCATAGA GAGAGGAGAT GAAAGCAGAA GAGGACAGAA AAATGGTCCC
    TTTTCAGAAG AGGCCCTATT GCCATACAAC AAACCATCAT TCCCATCCCC TGGTGGGCAC
    AGCTCATCTG GGACGGCCTC TTCTAAAGGG TCAACGGGTC CTCGCAAAGG AGAAGGGACG
    AGGGGACAGC ACCAGCGCTC CACCACGGAA CAAGGAGAAA GCAGCTACAT GGGGACCAAC
    GCGCAGTTTT CCGGAGAGTT ATGTGAGTCT GAACAAGTTC AAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206488.1
    CDS680..5467
    Translation

    Target ORF information:

    RefSeq Version XM_017350999.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017350999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT
    CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG
    CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG
    CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG
    CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC
    AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG
    GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC
    GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC
    CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT
    TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC
    TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT
    CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC
    TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC
    CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC
    ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA
    GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA
    GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA
    CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG
    TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA
    GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT
    CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC
    ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG
    CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC
    CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG
    AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT
    GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG
    GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG
    ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC
    ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC
    TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TTCAGTCGTT CACCTTCACC
    CCTGCAGTGA CGTTCCAGCG TGTTGATGGA GGCTTGATGA GCAACGGGAG TCGTCCTGGT
    CTGCTGAACG CCAGTGATTC GGGTTACCCG TGGCTCGCCG ACTCCTGGCC TCCCAGCCTG
    CCTGTCAACA GCGGATCTGG AGGACCCAAT GACCTCAGCT TTGGCCGTGG AGATGTGATG
    CCCAGTGCTC AGGCTGATAA GACGGGTACC ATGCTTTCGG ATGGAGCCAT TTACAGCAGC
    ATCGACTTCA CCACCAAAGG CGGATTCGGT AGCCCAAGCC CAGCCTCCCA GGCCACGCCC
    TATGCCACCA CACAGATCCT CCACTCCAAC AGCATCCATG AGCTGGCAGT GGATCTTCCT
    GAAGCCCAGT GGAAGACCTC CCTACAGGCC AAGCAGGAGA TGGCCAGCCT GGGTTACTCA
    CTGCCCGACA AAAACTCCTG CAATAACACA CTCCTTTTCA TTCCTGACTA CCGATTGGCT
    GACGGACTGT CTAATAGAAT GCCACACAAC CAATCGCAGG ATTTCAGCAC CACCAGCTCC
    CACAACAGCT CAGATCGGAG CAACAGTCTG TCAGGTGGAA AAGGTGGTAA GAAGAAGAAG
    ACCAAAGCAA ACGTCAAGCC CACCAAGATG AATGGCTCCA ACTGGGCGAA CGTTCCACTT
    CCTCCTCCTC CCATCCACCC TCTTCCTGGC ACAGAGATGG ACCACTACGC CTCAGAGCAC
    CATGATGGAG GAGGATATGA TAGTGATGGT TGGGGCCCAC CATTGCCAGT GCAGACGTAC
    CTCCATCAGG GTTTAGAGGA TGAGCTGGAG GAAGAGGAAG AGCGGGTGCC AACTCCTCCC
    ATTAGGGGTG TGGCTTCTTC TCCTGCTGCA GTTTCCTTTG GCAAACAGTC CACAGCCACA
    CTGAGCCCAT CTCCACGTGA AGAAATGCAG CCCATGCTCC AAGCCCACCT AGATGAGCTT
    ACCCGTGCTT ATCAATTGGA CATGGCTAAA CAGACTTGGC ACATGCAGGG TGGGCCTTAC
    CCGCCCCAGG CCCCCGCACC ACCCGTGGGC TACGTCTCCA GCACCTTAGT GTCTGACATC
    GAGACCGATC TCCCTGATGA AGATGACGAT GATGAGGAGG ATGAAGGATA CGAGATGTCG
    CCACCAATTA GGGGCACTGA ACACACGCCA GGCTCCAGCA TGGACAACTT AGACAGCTCC
    GTTACGGGCT CTATGGTGAA CGGCTGGGGC TCGGCTTCAG AAGATGATGA CAGAAACTTC
    TCCAGCAGGA GGTCTAGTGC CGGCAGCTCT TCTGACGGCT CGCTCTTCAC CCATTGCAGC
    TTTGCGCGAG CCCTGGTGGC AGCCGCAGAC AAGGCTGGTT ACCGTCTGGA TGGCACCAGC
    CTGAGCAAAA GAGATAAAGG TCTACCTCAT AGACCTCGTC CCGCTAGCCC GTTCTCCACA
    GATGGAAGCA CGGTGGGCAC ACACAGTCTG GGGCACCAGC GGGCAGCAAG GCCCACACGT
    AAACACAAAG GCCCTGGCAC TGTCCCGAAC CGCCGTGATG CCAGTGCGGA TGATCTGCCT
    CCACCTCCAG AGCCTCCACC CTGCCAGACA GCTCGCATCC CCGGGTCGAG GAACGTTCCC
    CATCCCTGCG ACCGCAAAGC TTCCTCTCTG GAGAGGCAGC CTATGATGGG CCTTGAGGAA
    ATGAGAAGTT CCTTGGACCG GCAGGCCCGA ACCTCATTGG ACCGCCACCA ACAAGTCCAG
    GACAGACTGG GGTCCATAGA GAGAGGAGAT GAAAGCAGAA GAGGACAGAA AAATGGTCCC
    TTTTCAGAAG AGGCCCTATT GCCATACAAC AAACCATCAT TCCCATCCCC TGGTGGGCAC
    AGCTCATCTG GGACGGCCTC TTCTAAAGGG TCAACGGGTC CTCGCAAAGG AGAAGGGACG
    AGGGGACAGC ACCAGCGCTC CACCACGGAA CAAGGAGAAA GCAGCTACAT GGGGACCAAC
    GCGCAGTTTT CCGGAGAGTT ATGTGAGTCT GAACAAGTTC AAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55119
    Clone ID Related Accession (Same CDS sequence) XM_017351000.1 , XM_017351000.2
    Accession Version XM_017351000.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206489.1
    CDS677..5452
    Translation

    Target ORF information:

    RefSeq Version XM_017351000.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55119
    Clone ID Related Accession (Same CDS sequence) XM_017351000.1 , XM_017351000.2
    Accession Version XM_017351000.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206489.1
    CDS638..5413
    Translation

    Target ORF information:

    RefSeq Version XM_017351000.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351000.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55120
    Clone ID Related Accession (Same CDS sequence) XM_017351001.1
    Accession Version XM_017351001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT
    AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT
    CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC
    AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC
    CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA
    TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA
    GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT
    TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA
    CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA
    TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC
    GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT
    GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC
    ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG
    GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT
    AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG
    GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT
    AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG
    GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT
    GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT
    CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC
    AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG
    GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC
    ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC
    CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG
    GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG
    CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA
    GAGTTATGTG AGTCTGAACA AGTTCAAGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206490.1
    CDS680..5452
    Translation

    Target ORF information:

    RefSeq Version XM_017351001.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT
    AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT
    CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC
    AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC
    CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA
    TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA
    GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT
    TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA
    CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA
    TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC
    GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT
    GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC
    ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG
    GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT
    AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG
    GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT
    AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG
    GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT
    GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT
    CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC
    AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG
    GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC
    ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC
    CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG
    GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG
    CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA
    GAGTTATGTG AGTCTGAACA AGTTCAAGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55121
    Clone ID Related Accession (Same CDS sequence) XM_017351002.1
    Accession Version XM_017351002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT
    TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC
    GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT
    GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT
    CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC
    CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT
    GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC
    ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG
    CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA
    TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC
    AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA
    TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG
    GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA
    GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATGTGA GTCTGAACAA
    GTTCAAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206491.1
    CDS616..5367
    Translation

    Target ORF information:

    RefSeq Version XM_017351002.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT
    TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC
    GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT
    GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT
    CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC
    CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT
    GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC
    ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG
    CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA
    TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC
    AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA
    TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG
    GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA
    GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATGTGA GTCTGAACAA
    GTTCAAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55122
    Clone ID Related Accession (Same CDS sequence) XM_017351003.1
    Accession Version XM_017351003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206492.1
    CDS1..4740
    Translation

    Target ORF information:

    RefSeq Version XM_017351003.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT
    GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC
    ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG
    GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC
    CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA
    AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG
    GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG
    AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG
    GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC
    CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG
    AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC
    CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA
    GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC
    ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55123
    Clone ID Related Accession (Same CDS sequence) XM_017351004.1
    Accession Version XM_017351004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA GTGGAAAAGG TGGTAAGAAG
    AAGAAGACCA AAGCAAACGT CAAGCCCACC AAGATGAATG GCTCCAACTG GGCGAACGTT
    CCACTTCCTC CTCCTCCCAT CCACCCTCTT CCTGGCACAG AGATGGACCA CTACGCCTCA
    GAGCACCATG ATGGAGGAGG ATATGATAGT GATGGTTGGG GCCCACCATT GCCAGTGCAG
    ACGTACCTCC ATCAGGGTTT AGAGGATGAG CTGGAGGAAG AGGAAGAGCG GGTGCCAACT
    CCTCCCATTA GGGGTGTGGC TTCTTCTCCT GCTGCAGTTT CCTTTGGCAA ACAGTCCACA
    GCCACACTGA GCCCATCTCC ACGTGAAGAA ATGCAGCCCA TGCTCCAAGC CCACCTAGAT
    GAGCTTACCC GTGCTTATCA ATTGGACATG GCTAAACAGA CTTGGCACAT GCAGGGTGGG
    CCTTACCCGC CCCAGGCCCC CGCACCACCC GTGGGCTACG TCTCCAGCAC CTTAGTGTCT
    GACATCGAGA CCGATCTCCC TGATGAAGAT GACGATGATG AGGAGGATGA AGGATACGAG
    ATGTCGCCAC CAATTAGGGG CACTGAACAC ACGCCAGGCT CCAGCATGGA CAACTTAGAC
    AGCTCCGTTA CGGGCTCTAT GGTGAACGGC TGGGGCTCGG CTTCAGAAGA TGATGACAGA
    AACTTCTCCA GCAGGAGGTC TAGTGCCGGC AGCTCTTCTG ACGGCTCGCT CTTCACCCAT
    TGCAGCTTTG CGCGAGCCCT GGTGGCAGCC GCAGACAAGG CTGGTTACCG TCTGGATGGC
    ACCAGCCTGA GCAAAAGAGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC
    TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC
    ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT
    CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC
    GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT
    GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA
    GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT
    GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT
    GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA
    GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG
    ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206493.1
    CDS680..5353
    Translation

    Target ORF information:

    RefSeq Version XM_017351004.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA GTGGAAAAGG TGGTAAGAAG
    AAGAAGACCA AAGCAAACGT CAAGCCCACC AAGATGAATG GCTCCAACTG GGCGAACGTT
    CCACTTCCTC CTCCTCCCAT CCACCCTCTT CCTGGCACAG AGATGGACCA CTACGCCTCA
    GAGCACCATG ATGGAGGAGG ATATGATAGT GATGGTTGGG GCCCACCATT GCCAGTGCAG
    ACGTACCTCC ATCAGGGTTT AGAGGATGAG CTGGAGGAAG AGGAAGAGCG GGTGCCAACT
    CCTCCCATTA GGGGTGTGGC TTCTTCTCCT GCTGCAGTTT CCTTTGGCAA ACAGTCCACA
    GCCACACTGA GCCCATCTCC ACGTGAAGAA ATGCAGCCCA TGCTCCAAGC CCACCTAGAT
    GAGCTTACCC GTGCTTATCA ATTGGACATG GCTAAACAGA CTTGGCACAT GCAGGGTGGG
    CCTTACCCGC CCCAGGCCCC CGCACCACCC GTGGGCTACG TCTCCAGCAC CTTAGTGTCT
    GACATCGAGA CCGATCTCCC TGATGAAGAT GACGATGATG AGGAGGATGA AGGATACGAG
    ATGTCGCCAC CAATTAGGGG CACTGAACAC ACGCCAGGCT CCAGCATGGA CAACTTAGAC
    AGCTCCGTTA CGGGCTCTAT GGTGAACGGC TGGGGCTCGG CTTCAGAAGA TGATGACAGA
    AACTTCTCCA GCAGGAGGTC TAGTGCCGGC AGCTCTTCTG ACGGCTCGCT CTTCACCCAT
    TGCAGCTTTG CGCGAGCCCT GGTGGCAGCC GCAGACAAGG CTGGTTACCG TCTGGATGGC
    ACCAGCCTGA GCAAAAGAGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC
    TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC
    ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT
    CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC
    GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT
    GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA
    GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT
    GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT
    GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA
    GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG
    ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55124
    Clone ID Related Accession (Same CDS sequence) XM_017351006.1
    Accession Version XM_017351006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC
    ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT
    CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT
    AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT
    GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT
    CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA
    GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC
    ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA
    CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA
    GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA
    CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC
    GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC
    GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA
    GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT
    CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA
    CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT
    GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC
    TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT
    TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG
    CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG
    AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG
    CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT
    TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC
    ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA
    TGTGAGTCTG AACAAGTTCA AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206495.1
    CDS680..5326
    Translation

    Target ORF information:

    RefSeq Version XM_017351006.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC
    CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT
    GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA
    TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT
    GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC
    AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG
    ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG
    ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC
    TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC
    ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT
    CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT
    AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT
    GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT
    CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA
    GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC
    ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA
    CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA
    GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA
    CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC
    GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC
    GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA
    GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT
    CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA
    CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT
    GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC
    TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT
    TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG
    CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG
    AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG
    CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT
    TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC
    ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA
    TGTGAGTCTG AACAAGTTCA AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55125
    Clone ID Related Accession (Same CDS sequence) XM_017351007.1
    Accession Version XM_017351007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC
    TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC
    ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT
    CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC
    GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT
    GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA
    GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT
    GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT
    GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA
    GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG
    ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206496.1
    CDS678..5291
    Translation

    Target ORF information:

    RefSeq Version XM_017351007.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC
    AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA
    GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA
    AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC
    ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC
    AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC
    ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG
    TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG
    CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA
    CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA
    TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT
    GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC
    GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA
    GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA
    ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG
    GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT
    ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT
    ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG
    GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC
    CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG
    GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT
    GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT
    GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC
    ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC
    TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC
    TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC
    ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC
    CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA
    CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC
    ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG
    TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG
    AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG
    CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT
    GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC
    ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC
    CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA
    GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC
    CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC
    TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC
    ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT
    AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG
    AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC
    GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA
    GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG
    CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG
    TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC
    CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG
    GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA
    GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA
    TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC
    TTAGACAGCT CCGTTACGGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC
    TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC
    ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT
    CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC
    GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT
    GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA
    GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT
    GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT
    GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA
    GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG
    ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55126
    Clone ID Related Accession (Same CDS sequence) XM_017351008.1
    Accession Version XM_017351008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT GTGAGTCTGA ACAAGTTCAA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206497.1
    CDS1..4566
    Translation

    Target ORF information:

    RefSeq Version XM_017351008.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA
    GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT
    CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC
    CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT GTGAGTCTGA ACAAGTTCAA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55127
    Clone ID Related Accession (Same CDS sequence) XM_017351009.1
    Accession Version XM_017351009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT
    CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG
    CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG
    CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG
    CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC
    AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG
    GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC
    GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC
    CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT
    TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC
    TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT
    CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC
    TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC
    CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC
    ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA
    GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA
    GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA
    CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG
    TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA
    GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT
    CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC
    ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG
    CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC
    CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG
    AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT
    GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG
    GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG
    ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC
    ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC
    TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT
    GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC
    CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC
    AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT
    ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC
    GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC
    AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG
    GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC
    ACACTCCTTT TCATTCCTGA CTACCGATTG GCTGACGGAC TGTCTAATAG AATGCCACAC
    AACCAATCGC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCAGATCG GAGCAACAGT
    CTGTCAGGTG GAAAAGGTGG TAAGAAGAAG AAGACCAAAG CAAACGTCAA GCCCACCAAG
    ATGAATGGCT CCAACTGGGC GAACGTTCCA CTTCCTCCTC CTCCCATCCA CCCTCTTCCT
    GGCACAGAGA TGGACCACTA CGCCTCAGAG CACCATGATG GAGGAGGATA TGATAGTGAT
    GGTTGGGGCC CACCATTGCC AGTGCAGACG TACCTCCATC AGGGTTTAGA GGATGAGCTG
    GAGGAAGAGG AAGAGCGGGT GCCAACTCCT CCCATTAGGG GTGTGGCTTC TTCTCCTGCT
    GCAGTTTCCT TTGGCAAACA GTCCACAGCC ACACTGAGCC CATCTCCACG TGAAGAAATG
    CAGCCCATGC TCCAAGCCCA CCTAGATGAG CTTACCCGTG CTTATCAATT GGACATGGCT
    AAACAGACTT GGCACATGCA GGGTGGGCCT TACCCGCCCC AGGCCCCCGC ACCACCCGTG
    GGCTACGTCT CCAGCACCTT AGTGTCTGAC ATCGAGACCG ATCTCCCTGA TGAAGATGAC
    GATGATGAGG AGGATGAAGG ATACGAGATG TCGCCACCAA TTAGGGGCAC TGAACACACG
    CCAGGCTCCA GCATGGACAA CTTAGACAGC TCCGTTACGG GCTCTATGGT GAACGGCTGG
    GGCTCGGCTT CAGAAGATGA TGACAGAAAC TTCTCCAGCA GGAGGTCTAG TGCCGGCAGC
    TCTTCTGACG GCTCGCTCTT CACCCATTGC AGCTTTGCGC GAGCCCTGGT GGCAGCCGCA
    GACAAGGCTG GTTACCGTCT GGATGGCACC AGCCTGAGCA AAAGAGATAA AGGTCTACCT
    CATAGACCTC GTCCCGCTAG CCCGTTCTCC ACAGATGGAA GCACGGTGGG CACACACAGT
    CTGGGGCACC AGCGGGCAGC AAGGCCCACA CGTAAACACA AAGGCCCTGG CACTGTCCCG
    AACCGCCGTG ATGCCAGTGC GGATGATCTG CCTCCACCTC CAGAGCCTCC ACCCTGCCAG
    ACAGCTCGCA TCCCCGGGTC GAGGAACGTT CCCCATCCCT GCGACCGCAA AGCTTCCTCT
    CTGGAGAGGC AGCCTATGAT GGGCCTTGAG GAAATGAGAA GTTCCTTGGA CCGGCAGGCC
    CGAACCTCAT TGGACCGCCA CCAACAAGTC CAGGACAGAC TGGGGTCCAT AGAGAGAGGA
    GATGAAAGCA GAAGAGGACA GAAAAATGGT CCCTTTTCAG AAGAGGCCCT ATTGCCATAC
    AACAAACCAT CATTCCCATC CCCTGGTGGG CACAGCTCAT CTGGGACGGC CTCTTCTAAA
    GGGTCAACGG GTCCTCGCAA AGGAGAAGGG ACGAGGGGAC AGCACCAGCG CTCCACCACG
    GAACAAGGAG AAAGCAGCTA CATGGGGACC AACGCGCAGT TTTCCGGAGA GTTATGTGAG
    TCTGAACAAG TTCAAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206498.1
    CDS680..5440
    Translation

    Target ORF information:

    RefSeq Version XM_017351009.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT
    CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG
    CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG
    CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG
    CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC
    AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG
    GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC
    GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC
    CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT
    TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC
    TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT
    CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC
    TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC
    CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC
    ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA
    GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA
    GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA
    CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG
    TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA
    GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT
    CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC
    ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG
    CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC
    CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG
    AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT
    GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG
    GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG
    ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC
    ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC
    TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT
    GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC
    CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC
    AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT
    ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC
    GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC
    AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG
    GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC
    ACACTCCTTT TCATTCCTGA CTACCGATTG GCTGACGGAC TGTCTAATAG AATGCCACAC
    AACCAATCGC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCAGATCG GAGCAACAGT
    CTGTCAGGTG GAAAAGGTGG TAAGAAGAAG AAGACCAAAG CAAACGTCAA GCCCACCAAG
    ATGAATGGCT CCAACTGGGC GAACGTTCCA CTTCCTCCTC CTCCCATCCA CCCTCTTCCT
    GGCACAGAGA TGGACCACTA CGCCTCAGAG CACCATGATG GAGGAGGATA TGATAGTGAT
    GGTTGGGGCC CACCATTGCC AGTGCAGACG TACCTCCATC AGGGTTTAGA GGATGAGCTG
    GAGGAAGAGG AAGAGCGGGT GCCAACTCCT CCCATTAGGG GTGTGGCTTC TTCTCCTGCT
    GCAGTTTCCT TTGGCAAACA GTCCACAGCC ACACTGAGCC CATCTCCACG TGAAGAAATG
    CAGCCCATGC TCCAAGCCCA CCTAGATGAG CTTACCCGTG CTTATCAATT GGACATGGCT
    AAACAGACTT GGCACATGCA GGGTGGGCCT TACCCGCCCC AGGCCCCCGC ACCACCCGTG
    GGCTACGTCT CCAGCACCTT AGTGTCTGAC ATCGAGACCG ATCTCCCTGA TGAAGATGAC
    GATGATGAGG AGGATGAAGG ATACGAGATG TCGCCACCAA TTAGGGGCAC TGAACACACG
    CCAGGCTCCA GCATGGACAA CTTAGACAGC TCCGTTACGG GCTCTATGGT GAACGGCTGG
    GGCTCGGCTT CAGAAGATGA TGACAGAAAC TTCTCCAGCA GGAGGTCTAG TGCCGGCAGC
    TCTTCTGACG GCTCGCTCTT CACCCATTGC AGCTTTGCGC GAGCCCTGGT GGCAGCCGCA
    GACAAGGCTG GTTACCGTCT GGATGGCACC AGCCTGAGCA AAAGAGATAA AGGTCTACCT
    CATAGACCTC GTCCCGCTAG CCCGTTCTCC ACAGATGGAA GCACGGTGGG CACACACAGT
    CTGGGGCACC AGCGGGCAGC AAGGCCCACA CGTAAACACA AAGGCCCTGG CACTGTCCCG
    AACCGCCGTG ATGCCAGTGC GGATGATCTG CCTCCACCTC CAGAGCCTCC ACCCTGCCAG
    ACAGCTCGCA TCCCCGGGTC GAGGAACGTT CCCCATCCCT GCGACCGCAA AGCTTCCTCT
    CTGGAGAGGC AGCCTATGAT GGGCCTTGAG GAAATGAGAA GTTCCTTGGA CCGGCAGGCC
    CGAACCTCAT TGGACCGCCA CCAACAAGTC CAGGACAGAC TGGGGTCCAT AGAGAGAGGA
    GATGAAAGCA GAAGAGGACA GAAAAATGGT CCCTTTTCAG AAGAGGCCCT ATTGCCATAC
    AACAAACCAT CATTCCCATC CCCTGGTGGG CACAGCTCAT CTGGGACGGC CTCTTCTAAA
    GGGTCAACGG GTCCTCGCAA AGGAGAAGGG ACGAGGGGAC AGCACCAGCG CTCCACCACG
    GAACAAGGAG AAAGCAGCTA CATGGGGACC AACGCGCAGT TTTCCGGAGA GTTATGTGAG
    TCTGAACAAG TTCAAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55128
    Clone ID Related Accession (Same CDS sequence) XM_017351010.1
    Accession Version XM_017351010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 2 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT
    CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG
    CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG
    CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG
    CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC
    AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG
    GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC
    GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC
    CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT
    TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC
    TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT
    CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC
    TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC
    CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC
    ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA
    GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA
    GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA
    CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG
    TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA
    GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT
    CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC
    ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG
    CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC
    CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG
    AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT
    GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG
    GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG
    ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC
    ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC
    TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT
    GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC
    CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC
    AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT
    ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC
    GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC
    AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG
    GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC
    AGTGGAAAAG GTGGTAAGAA GAAGAAGACC AAAGCAAACG TCAAGCCCAC CAAGATGAAT
    GGCTCCAACT GGGCGAACGT TCCACTTCCT CCTCCTCCCA TCCACCCTCT TCCTGGCACA
    GAGATGGACC ACTACGCCTC AGAGCACCAT GATGGAGGAG GATATGATAG TGATGGTTGG
    GGCCCACCAT TGCCAGTGCA GACGTACCTC CATCAGGGTT TAGAGGATGA GCTGGAGGAA
    GAGGAAGAGC GGGTGCCAAC TCCTCCCATT AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT
    TCCTTTGGCA AACAGTCCAC AGCCACACTG AGCCCATCTC CACGTGAAGA AATGCAGCCC
    ATGCTCCAAG CCCACCTAGA TGAGCTTACC CGTGCTTATC AATTGGACAT GGCTAAACAG
    ACTTGGCACA TGCAGGGTGG GCCTTACCCG CCCCAGGCCC CCGCACCACC CGTGGGCTAC
    GTCTCCAGCA CCTTAGTGTC TGACATCGAG ACCGATCTCC CTGATGAAGA TGACGATGAT
    GAGGAGGATG AAGGATACGA GATGTCGCCA CCAATTAGGG GCACTGAACA CACGCCAGGC
    TCCAGCATGG ACAACTTAGA CAGCTCCGTT ACGGGCTCTA TGGTGAACGG CTGGGGCTCG
    GCTTCAGAAG ATGATGACAG AAACTTCTCC AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT
    GACGGCTCGC TCTTCACCCA TTGCAGCTTT GCGCGAGCCC TGGTGGCAGC CGCAGACAAG
    GCTGGTTACC GTCTGGATGG CACCAGCCTG AGCAAAAGAG ATAAAGGTCT ACCTCATAGA
    CCTCGTCCCG CTAGCCCGTT CTCCACAGAT GGAAGCACGG TGGGCACACA CAGTCTGGGG
    CACCAGCGGG CAGCAAGGCC CACACGTAAA CACAAAGGCC CTGGCACTGT CCCGAACCGC
    CGTGATGCCA GTGCGGATGA TCTGCCTCCA CCTCCAGAGC CTCCACCCTG CCAGACAGCT
    CGCATCCCCG GGTCGAGGAA CGTTCCCCAT CCCTGCGACC GCAAAGCTTC CTCTCTGGAG
    AGGCAGCCTA TGATGGGCCT TGAGGAAATG AGAAGTTCCT TGGACCGGCA GGCCCGAACC
    TCATTGGACC GCCACCAACA AGTCCAGGAC AGACTGGGGT CCATAGAGAG AGGAGATGAA
    AGCAGAAGAG GACAGAAAAA TGGTCCCTTT TCAGAAGAGG CCCTATTGCC ATACAACAAA
    CCATCATTCC CATCCCCTGG TGGGCACAGC TCATCTGGGA CGGCCTCTTC TAAAGGGTCA
    ACGGGTCCTC GCAAAGGAGA AGGGACGAGG GGACAGCACC AGCGCTCCAC CACGGAACAA
    GGAGAAAGCA GCTACATGGG GACCAACGCG CAGTTTTCCG GAGAGTTATG TGAGTCTGAA
    CAAGTTCAAG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206499.1
    CDS680..5314
    Translation

    Target ORF information:

    RefSeq Version XM_017351010.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
    CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG
    CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC
    GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC
    AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT
    CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG
    GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG
    CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT
    GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC
    TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA
    GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC
    ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC
    ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA
    TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC
    CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG
    GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG
    GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT
    CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG
    CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG
    CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG
    CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC
    AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG
    GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC
    GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC
    CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT
    TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC
    TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT
    CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC
    TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC
    CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC
    ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA
    GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA
    GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA
    CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG
    TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA
    GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT
    CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC
    ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG
    CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC
    CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG
    AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT
    GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG
    GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG
    ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC
    ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC
    TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT
    GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC
    CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC
    AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT
    ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC
    GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC
    AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG
    GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC
    AGTGGAAAAG GTGGTAAGAA GAAGAAGACC AAAGCAAACG TCAAGCCCAC CAAGATGAAT
    GGCTCCAACT GGGCGAACGT TCCACTTCCT CCTCCTCCCA TCCACCCTCT TCCTGGCACA
    GAGATGGACC ACTACGCCTC AGAGCACCAT GATGGAGGAG GATATGATAG TGATGGTTGG
    GGCCCACCAT TGCCAGTGCA GACGTACCTC CATCAGGGTT TAGAGGATGA GCTGGAGGAA
    GAGGAAGAGC GGGTGCCAAC TCCTCCCATT AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT
    TCCTTTGGCA AACAGTCCAC AGCCACACTG AGCCCATCTC CACGTGAAGA AATGCAGCCC
    ATGCTCCAAG CCCACCTAGA TGAGCTTACC CGTGCTTATC AATTGGACAT GGCTAAACAG
    ACTTGGCACA TGCAGGGTGG GCCTTACCCG CCCCAGGCCC CCGCACCACC CGTGGGCTAC
    GTCTCCAGCA CCTTAGTGTC TGACATCGAG ACCGATCTCC CTGATGAAGA TGACGATGAT
    GAGGAGGATG AAGGATACGA GATGTCGCCA CCAATTAGGG GCACTGAACA CACGCCAGGC
    TCCAGCATGG ACAACTTAGA CAGCTCCGTT ACGGGCTCTA TGGTGAACGG CTGGGGCTCG
    GCTTCAGAAG ATGATGACAG AAACTTCTCC AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT
    GACGGCTCGC TCTTCACCCA TTGCAGCTTT GCGCGAGCCC TGGTGGCAGC CGCAGACAAG
    GCTGGTTACC GTCTGGATGG CACCAGCCTG AGCAAAAGAG ATAAAGGTCT ACCTCATAGA
    CCTCGTCCCG CTAGCCCGTT CTCCACAGAT GGAAGCACGG TGGGCACACA CAGTCTGGGG
    CACCAGCGGG CAGCAAGGCC CACACGTAAA CACAAAGGCC CTGGCACTGT CCCGAACCGC
    CGTGATGCCA GTGCGGATGA TCTGCCTCCA CCTCCAGAGC CTCCACCCTG CCAGACAGCT
    CGCATCCCCG GGTCGAGGAA CGTTCCCCAT CCCTGCGACC GCAAAGCTTC CTCTCTGGAG
    AGGCAGCCTA TGATGGGCCT TGAGGAAATG AGAAGTTCCT TGGACCGGCA GGCCCGAACC
    TCATTGGACC GCCACCAACA AGTCCAGGAC AGACTGGGGT CCATAGAGAG AGGAGATGAA
    AGCAGAAGAG GACAGAAAAA TGGTCCCTTT TCAGAAGAGG CCCTATTGCC ATACAACAAA
    CCATCATTCC CATCCCCTGG TGGGCACAGC TCATCTGGGA CGGCCTCTTC TAAAGGGTCA
    ACGGGTCCTC GCAAAGGAGA AGGGACGAGG GGACAGCACC AGCGCTCCAC CACGGAACAA
    GGAGAAAGCA GCTACATGGG GACCAACGCG CAGTTTTCCG GAGAGTTATG TGAGTCTGAA
    CAAGTTCAAG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa02796
    Clone ID Related Accession (Same CDS sequence) NM_131633.1
    Accession Version NM_131633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 2 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF337035.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF337035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCCCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ACTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GGTGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG GAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACCA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCTAGTGGA
    GATCTAACCA TCACGGCGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCA GTGAGCAACA GCTGGCAGAC TGTTGCCGAT
    CACGTCAAAA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGGTGATC ACAGACATGT GCAGAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CGGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCGCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATA
    CATGAGCTAG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCTA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC AGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC ATACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACATC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TTCCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGGAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGTGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571708.1
    CDS168..4709
    Translation

    Target ORF information:

    RefSeq Version NM_131633.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
    TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC
    GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC
    GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCCCAC
    CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC
    AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT
    CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT
    GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG
    GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC
    AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC
    ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG
    TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ACTAACCAGG TGGTTCTGGA GGAGGAGGCT
    GTGGAATTTC GGTGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC
    GACGTTGAAA TCCCCCGCGG GAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG
    AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA
    GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG
    CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC
    TGCGAGACCA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT
    CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCTAGTGGA
    GATCTAACCA TCACGGCGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG
    ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT
    CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG
    GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC
    GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG
    ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT
    GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT
    AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC
    ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT
    ACATATGTCA TAGAAGCATT CAGCCAATCA GTGAGCAACA GCTGGCAGAC TGTTGCCGAT
    CACGTCAAAA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC
    ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC
    CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGGTGATC ACAGACATGT GCAGAAGGAG
    CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG
    GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG
    CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA
    GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT
    CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG
    GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CGGTGGGCAA CCAGAACAGC
    ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG
    GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC
    GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG
    GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT
    GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG
    GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG
    ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC
    GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG
    ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC
    GCCGACTCCT GGCCGCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC
    AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT
    TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA
    AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATA
    CATGAGCTAG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG
    GAGATGGCTA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT
    TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT
    GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC
    TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC AGGCACAGAG
    ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC
    CCACCATTGC CAGTGCAGAC ATACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG
    GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC
    TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG
    CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT
    TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACATC
    TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG
    GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC
    AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TTCCTCATAG ACCTCGTCCC
    GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG
    GCAGCAAGGC CCACACGGAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC
    AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC
    GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT
    ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC
    CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA
    GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC
    CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT
    CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGTGAAAGC
    AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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