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SUPT5H cDNA ORF clone, Danio rerio(Zebrafish)

Summary

Gene Symbol SUPT5H
Entrez Gene ID 64275
Full Name SPT5 homolog, DSIF elongation factor subunit
Synonyms Spt5,etID10129.15,etID37101.15,fb37g01,fb95e05,fog,foggy,wu:fb37g01,wu:fb95e05,wu:fc10f02
General protein information
Preferred Names

SPT5 homolog, DSIF elongation factor subunit

Names

transcription elongation factor SPT5
DRB sensitivity-inducing factor large subunit
DSIF large subunit
protein foggy
suppressor of Ty 5 homolog
transcription elongation regulator foggy
Gene Type protein-coding
Organism Danio rerio(Zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_021481463.1 XP_021337138.1 transcription elongation factor SPT5 isoform X2
XM_005157529.4 XP_005157586.1 transcription elongation factor SPT5 isoform X1
NM_131673.2 NP_571748.2 transcription elongation factor SPT5
XM_017351012.1 XP_017206501.1 transcription elongation factor SPT5 isoform X2
XM_005157529.3 XP_005157586.1 transcription elongation factor SPT5 isoform X1
XM_021481462.1 XP_021337137.1 transcription elongation factor SPT5 isoform X1
XM_005157530.3 XP_005157587.1 transcription elongation factor SPT5 isoform X1

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP005021 XP_001231045.2
Caenorhabditis elegans (roundworm) spt-5 NP_502283.1
Xenopus tropicalis (tropical clawed frog) supt5h NP_001123796.1
Canis lupus familiaris (dog) SUPT5H XP_005616644.1
Mus musculus (house mouse) Supt5 NP_038704.1
Gallus gallus (chicken) SUPT5H NP_001026555.1
Neurospora crassa NCU06929 XP_959276.2
SUPT5H XP_001088495.1
Bos taurus (cattle) SUPT5H NP_001076908.2
Rattus norvegicus (Norway rat) Supt5h NP_001100967.2
Schizosaccharomyces pombe (fission yeast) spt5 NP_593191.1
Arabidopsis thaliana (thale cress) AT2G34210 NP_180968.2
Oryza sativa (rice) Os02g0772000 NP_001173159.1
Saccharomyces cerevisiae (baker's yeast) SPT5 NP_013703.1
Eremothecium gossypii AGOS_ADR187W NP_984283.2
Homo sapiens (human) SUPT5H NP_001104490.1
Pan troglodytes (chimpanzee) SUPT5H XP_512652.3
Danio rerio (zebrafish) supt5h NP_571748.2
Drosophila melanogaster (fruit fly) Spt5 NP_652610.1
Kluyveromyces lactis KLLA0B00891g XP_451570.1
Magnaporthe oryzae (rice blast fungus) MGG_12268 XP_003715018.1
Arabidopsis thaliana (thale cress) GTA2 NP_192575.3

Gene Ontology


Bibliography

Related articles in PubMed

Maternal methylmercury from a wild-caught walleye diet induces developmental abnormalities in zebrafish.
Liu Q, Klingler RH, Wimpee B, Dellinger M, King-Heiden T, Grzybowski J, Gerstenberger SL, Weber DN, Carvan MJ 3rd
Reproductive toxicology (Elmsford, N.Y.)65272-282(2016 10)

RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Yang Q, Liu X, Zhou T, Cook J, Nguyen K, Bai X
Blood128(13)1701-10(2016 09)

Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation.
Taneda T, Zhu W, Cao Q, Watanabe H, Yamaguchi Y, Handa H, Wada T
Genes to cells : devoted to molecular & cellular mechanisms16(2)231-42(2011 Feb)

TIF1gamma controls erythroid cell fate by regulating transcription elongation.
Bai X, Kim J, Yang Z, Jurynec MJ, Akie TE, Lee J, LeBlanc J, Sessa A, Jiang H, DiBiase A, Zhou Y, Grunwald DJ, Lin S, Cantor AB, Orkin SH, Zon LI
Cell142(1)133-43(2010 Jul)

Repression of RNA polymerase II elongation in vivo is critically dependent on the C-terminus of Spt5.
Chen H, Contreras X, Yamaguchi Y, Handa H, Peterlin BM, Guo S
PloS one4(9)e6918(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the positive function of Foggy/Spt5 is required for gata1 expression during zebrafish embryonic hematopoiesis.
Title: Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation.

RNA polymerase II elongation repression is critically dependent on the C-terminus of Spt5
Title: Repression of RNA polymerase II elongation in vivo is critically dependent on the C-terminus of Spt5.

Spt5 as a dual regulator of transcription elongation in vivo; a small but diverse set of target genes critically dependent on Spt5 during development.
Title: Identification of Spt5 target genes in zebrafish development reveals its dual activity in vivo.

spt 5-dependent transcript elongation is required cell-autonomously for a complex cell migration
Title: Zebrafish foggy/spt 5 is required for migration of facial branchiomotor neurons but not for their survival.

The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebrafish embryo.
Title: The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebrafish embryo.

The following SUPT5H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SUPT5H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa38948 NM_131673.2
Latest version!		 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
ODa20394 XM_005157529.4
Latest version!		 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
ODa20394 XM_005157530.3
Latest version!		 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
ODa20394 XM_021481462.1
Latest version!		 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
ODa38948 XM_017351012.1
Latest version!		 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
ODa38948 XM_021481463.1
Latest version!		 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
ODa20394 XM_005157529.3 Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa38948
Clone ID Related Accession (Same CDS sequence) XM_021481463.1 , NM_131673.2 , XM_017351012.1
Accession Version NM_131673.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-19
Organism Danio rerio(zebrafish)
Product transcription elongation factor SPT5
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR626887.6. On May 7, 2009 this sequence version replaced NM_131673.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF288409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END## 

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2161
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2281
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ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGAAGAGG CTGAAGTGTC CAACCTTGAC CATGTAGTTC TGGATGAGGA TCACTCGGGT 
TCAAGGAGGT TGCAGAATCT CTGGAGAGAT TCTCGTGAGG AAGCTCTGGG GGAGTATTAC 
ATGAGGAAAT ATGCCAAGTC TTCAGGAGGC GAACACTTCT ATGGTGGGTC TGAGGATCTT 
TCTGATGACA TCACACAGCA ACAATTGCTT CCTGGGGTCA AGGATCCTAA TTTGTGGACT 
GTTAAGTGTA AGATTGGAGA GGAGAGAGCA ACTGCAATTT CCTTGATGCG AAAATTCGTT 
GCTTATCAGT GCACTGACAC GCCACTTCAA ATCAAATCAG TAGTTGCCCC TGAACATGTC 
AAAGGTTATA TCTATGTGGA GGCGTACAAG CAGACCCATG TCAAGGCTGC CATTGAGGGT 
GTGGGAAACC TGCGCATGGG CTTCTGGAAC CAGCAGATGG TGCCCATTAA AGAGATGACA 
GATGTGCTGA AAGTGGTTAA GGAAGTGACT AACCTTAAAC CCAAGTCTTG GGTTCGTCTC 
AAGAGAGGCC TTTATAAAGA TGACATTGCC CAGGTGGACT ATGTTGAACC TAGTCAGAAC 
ACGATTTCGT TGAAAATGAT TCCTCGTATT GACCTGGATC GTATCAAAGC AAGGATGAGT 
ATGAAAGACT GGTTTGCAAA GAGAAAGAAG TTCAAGAGGC CCCCACAGAG GCTTTTTGAT 
GCTGAAAAGA TCAGGTCTCT TGGTGGTGAG GTCAGCCATG ATGGTGATTT CATGATATTT 
GAAGCGAACC GCTACAGCAG AAAGGGTTTC CTGTTCAAAA GCTTTGCCAT GTCTGCTGTG 
ATCACAGAAG GGGTGAAGCC CACTCTGTCC GAGCTGGAGA AGTTTGAAGA CCAGCCAGAG 
GGCATTGACC TTGAAGTAGT CACAGAAACA ACAGGTAAGG AGCGAGAACA CAATCTCCAG 
GCGGGAGATA ATGTGGAGGT GTGTGAAGGG GAGTTGATCA ACCTTCAGGG CAAAATCCTG 
AGTGTGGATG GCAACAAAAT CACCATCATG CCCAAGCACG AGGACCTCAA GGATCCTCTT 
GAGTTTCCAG CTCATGAGTT GCGGAAGTAT TTTCGTATGG GTGACCATGT GAAGGTCATC 
GCGGGGCGTT ATGAAGGGGA TACAGGCCTC ATAGTTCGCG TGGAGGAGAA CTTTGTCATT 
CTGTTCTCTG ACCTCACCAT GCACGAGTTG AAAGTTTTGC CCCGGGACCT GCAGCTGTGC 
TCTGAGACGG CTTCAGGTGT GGATGCTGGA GGCCAGCATG AGTGGGGGGA GCTGGTACAG 
TTAGACCCTC AAACTGTAGG TGTCATTGTC AGGCTGGAGA GAGAGACCTT TCAGGTTCTG 
AACATGCATG GGAAAGTTTT AACAGTACGG CACCAGGCAG TGAACAGGCG GAAGGACAAC 
CGGTTTGCTG TCGCTCTCGA CTCTGAGCAA AACAACATCC ATGTTAAGGA TATTGTGAAA 
GTCATTGATG GACCTCATTC GGGCCGTGAA GGTGAAATTC GCCACATCTT CAGAGGCTTT 
GCTTTTCTTC ACTGCAAGAA GCTGGTTGAA AACGGAGGCA TGTTTGTTTG TAAAGCACGT 
CACCTTGTAC TGGCAGGTGG CTCTAAGCCC AGAGATGTGA CCAATTTTAC AGTGGGTGGC 
TTTGCTCCCA TGAGCCCACG CATCAGCAGC CCAATGCACC CTGGAGGAGG TGGTCAGCCG 
CAAAGAGGTG GAGGTGGTGG TGGTGGTGGT GGCATGGGCC GAGGCCGTGG ACGACGAGAC 
AATGATCTCA TTGGCCAGAC TGTTCGTATA TCCCAGGGAC CATACAAAGG CTACATTGGT 
GTGGTGAAGG ATGCCACAGA ATCCACCGCT AGGGTAGAGC TCCACTCAAC CTGCCAGACC 
ATCTCTGTGG ATCGCCAGCG CCTTACAACT GTTGGTGGCA AAGAAAGACA AGGAAGAAGT 
TCCACCCATC TACGAACCCC GATGTATGGT TCCCAGACAC CCATTTATGG GACTGGCTCA 
CGTACTCCCA TGTATGGTAG CCAGACACCC CTCCATGATG GAAGTCGTAC TCCTCACTAT 
GGCTCACAGA CTCCTCTTCA TGATGGGAGC AGGACACCTG GCCAAAGTGG TGCGTGGGAC 
CCCAACAATC CAAACACACC ATCACGGCCT GATGACGAGT ATGAGTTTGC CTACGATGAC 
GAACCCTCGC CGTCTCCTCA AGGCTATGGT GGGACACCCA ACCCTCAGAC ACCTGGATAT 
CCAGAAGTGC CCTCTCCGCA GGTCAACCCT CAGTATAACC CACAGACTCC TGGGACTCCT 
GCCATGTACA ACACAGAGCA ATACTCCCCA TATGCAGCAC CTTCACCCCA GGGCTCCTAC 
CAGCCCAGTC CAAGTCCACA GAGTTACCAC CAAGTGGCGC CAAGCCCGGT GGGATACCAA 
AACACACACT CGCCTGCCAG TTACCACCCC ACTCCATCAC CCATGGCTTA CCAGGCCAGT 
CCAAGTCCCA GTCCGGTAGG GTATAGTCCC ATGACTCCTG GCGCTCCGTC TCCTGGGGGT 
TACAACCCTC ACACCCCTGG CTCCAACATT GACCAAGCCT CCAATGACTG GGTCACCACA 
GACATCATGG TGCGTGTGAA AGACACATTC CTTGATGGAG GCGTCATCAA TCAGACCGGC 
ATCATCCGCA GTGTCACCGG AGGAATGTGC TCTGTTTTTC TGCAAGACAC TGAAAAGGTT 
GTTAGCATTT CAAGCGAACA CTTGGAGCCT GTTACACCAA CCAAAAACAA TAAGGTGAAG 
GTGATTCTGG GAGAGGACAG GGAGGCCACA GGAGTCCTGC TTAGTATCGA TGGTGAAGAC 
GGCATTGTTA GAATGGAGTT GGACGAGCAG CTGAAGATTC TCAATCTCCG CTTCCTTGGG 
AAACTGGAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_571748.2
CDS120..3374
Translation

Target ORF information:

RefSeq Version NM_131673.2
Organism Danio rerio(zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_131673.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGAAGAGG CTGAAGTGTC CAACCTTGAC CATGTAGTTC TGGATGAGGA TCACTCGGGT 
TCAAGGAGGT TGCAGAATCT CTGGAGAGAT TCTCGTGAGG AAGCTCTGGG GGAGTATTAC 
ATGAGGAAAT ATGCCAAGTC TTCAGGAGGC GAACACTTCT ATGGTGGGTC TGAGGATCTT 
TCTGATGACA TCACACAGCA ACAATTGCTT CCTGGGGTCA AGGATCCTAA TTTGTGGACT 
GTTAAGTGTA AGATTGGAGA GGAGAGAGCA ACTGCAATTT CCTTGATGCG AAAATTCGTT 
GCTTATCAGT GCACTGACAC GCCACTTCAA ATCAAATCAG TAGTTGCCCC TGAACATGTC 
AAAGGTTATA TCTATGTGGA GGCGTACAAG CAGACCCATG TCAAGGCTGC CATTGAGGGT 
GTGGGAAACC TGCGCATGGG CTTCTGGAAC CAGCAGATGG TGCCCATTAA AGAGATGACA 
GATGTGCTGA AAGTGGTTAA GGAAGTGACT AACCTTAAAC CCAAGTCTTG GGTTCGTCTC 
AAGAGAGGCC TTTATAAAGA TGACATTGCC CAGGTGGACT ATGTTGAACC TAGTCAGAAC 
ACGATTTCGT TGAAAATGAT TCCTCGTATT GACCTGGATC GTATCAAAGC AAGGATGAGT 
ATGAAAGACT GGTTTGCAAA GAGAAAGAAG TTCAAGAGGC CCCCACAGAG GCTTTTTGAT 
GCTGAAAAGA TCAGGTCTCT TGGTGGTGAG GTCAGCCATG ATGGTGATTT CATGATATTT 
GAAGCGAACC GCTACAGCAG AAAGGGTTTC CTGTTCAAAA GCTTTGCCAT GTCTGCTGTG 
ATCACAGAAG GGGTGAAGCC CACTCTGTCC GAGCTGGAGA AGTTTGAAGA CCAGCCAGAG 
GGCATTGACC TTGAAGTAGT CACAGAAACA ACAGGTAAGG AGCGAGAACA CAATCTCCAG 
GCGGGAGATA ATGTGGAGGT GTGTGAAGGG GAGTTGATCA ACCTTCAGGG CAAAATCCTG 
AGTGTGGATG GCAACAAAAT CACCATCATG CCCAAGCACG AGGACCTCAA GGATCCTCTT 
GAGTTTCCAG CTCATGAGTT GCGGAAGTAT TTTCGTATGG GTGACCATGT GAAGGTCATC 
GCGGGGCGTT ATGAAGGGGA TACAGGCCTC ATAGTTCGCG TGGAGGAGAA CTTTGTCATT 
CTGTTCTCTG ACCTCACCAT GCACGAGTTG AAAGTTTTGC CCCGGGACCT GCAGCTGTGC 
TCTGAGACGG CTTCAGGTGT GGATGCTGGA GGCCAGCATG AGTGGGGGGA GCTGGTACAG 
TTAGACCCTC AAACTGTAGG TGTCATTGTC AGGCTGGAGA GAGAGACCTT TCAGGTTCTG 
AACATGCATG GGAAAGTTTT AACAGTACGG CACCAGGCAG TGAACAGGCG GAAGGACAAC 
CGGTTTGCTG TCGCTCTCGA CTCTGAGCAA AACAACATCC ATGTTAAGGA TATTGTGAAA 
GTCATTGATG GACCTCATTC GGGCCGTGAA GGTGAAATTC GCCACATCTT CAGAGGCTTT 
GCTTTTCTTC ACTGCAAGAA GCTGGTTGAA AACGGAGGCA TGTTTGTTTG TAAAGCACGT 
CACCTTGTAC TGGCAGGTGG CTCTAAGCCC AGAGATGTGA CCAATTTTAC AGTGGGTGGC 
TTTGCTCCCA TGAGCCCACG CATCAGCAGC CCAATGCACC CTGGAGGAGG TGGTCAGCCG 
CAAAGAGGTG GAGGTGGTGG TGGTGGTGGT GGCATGGGCC GAGGCCGTGG ACGACGAGAC 
AATGATCTCA TTGGCCAGAC TGTTCGTATA TCCCAGGGAC CATACAAAGG CTACATTGGT 
GTGGTGAAGG ATGCCACAGA ATCCACCGCT AGGGTAGAGC TCCACTCAAC CTGCCAGACC 
ATCTCTGTGG ATCGCCAGCG CCTTACAACT GTTGGTGGCA AAGAAAGACA AGGAAGAAGT 
TCCACCCATC TACGAACCCC GATGTATGGT TCCCAGACAC CCATTTATGG GACTGGCTCA 
CGTACTCCCA TGTATGGTAG CCAGACACCC CTCCATGATG GAAGTCGTAC TCCTCACTAT 
GGCTCACAGA CTCCTCTTCA TGATGGGAGC AGGACACCTG GCCAAAGTGG TGCGTGGGAC 
CCCAACAATC CAAACACACC ATCACGGCCT GATGACGAGT ATGAGTTTGC CTACGATGAC 
GAACCCTCGC CGTCTCCTCA AGGCTATGGT GGGACACCCA ACCCTCAGAC ACCTGGATAT 
CCAGAAGTGC CCTCTCCGCA GGTCAACCCT CAGTATAACC CACAGACTCC TGGGACTCCT 
GCCATGTACA ACACAGAGCA ATACTCCCCA TATGCAGCAC CTTCACCCCA GGGCTCCTAC 
CAGCCCAGTC CAAGTCCACA GAGTTACCAC CAAGTGGCGC CAAGCCCGGT GGGATACCAA 
AACACACACT CGCCTGCCAG TTACCACCCC ACTCCATCAC CCATGGCTTA CCAGGCCAGT 
CCAAGTCCCA GTCCGGTAGG GTATAGTCCC ATGACTCCTG GCGCTCCGTC TCCTGGGGGT 
TACAACCCTC ACACCCCTGG CTCCAACATT GACCAAGCCT CCAATGACTG GGTCACCACA 
GACATCATGG TGCGTGTGAA AGACACATTC CTTGATGGAG GCGTCATCAA TCAGACCGGC 
ATCATCCGCA GTGTCACCGG AGGAATGTGC TCTGTTTTTC TGCAAGACAC TGAAAAGGTT 
GTTAGCATTT CAAGCGAACA CTTGGAGCCT GTTACACCAA CCAAAAACAA TAAGGTGAAG 
GTGATTCTGG GAGAGGACAG GGAGGCCACA GGAGTCCTGC TTAGTATCGA TGGTGAAGAC 
GGCATTGTTA GAATGGAGTT GGACGAGCAG CTGAAGATTC TCAATCTCCG CTTCCTTGGG 
AAACTGGAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20394
Clone ID Related Accession (Same CDS sequence) XM_005157529.4 , XM_005157529.3 , XM_021481462.1 , XM_005157530.3
Accession Version XM_005157529.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product transcription elongation factor SPT5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157529.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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481
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661
721
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841
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961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157586.1
CDS161..3421
Translation

Target ORF information:

RefSeq Version XM_005157529.4
Organism Danio rerio(zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157529.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20394
Clone ID Related Accession (Same CDS sequence) XM_005157529.4 , XM_005157529.3 , XM_021481462.1 , XM_005157530.3
Accession Version XM_005157529.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product transcription elongation factor SPT5 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157586.1
CDS216..3476
Translation

Target ORF information:

RefSeq Version XM_005157529.3
Organism Danio rerio(Zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157529.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20394
Clone ID Related Accession (Same CDS sequence) XM_005157529.4 , XM_005157529.3 , XM_021481462.1 , XM_005157530.3
Accession Version XM_021481462.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product transcription elongation factor SPT5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_021337137.1
CDS105..3365
Translation

Target ORF information:

RefSeq Version XM_021481462.1
Organism Danio rerio(zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_021481462.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20394
Clone ID Related Accession (Same CDS sequence) XM_005157529.4 , XM_005157529.3 , XM_021481462.1 , XM_005157530.3
Accession Version XM_005157530.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product transcription elongation factor SPT5 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157587.1
CDS157..3417
Translation

Target ORF information:

RefSeq Version XM_005157530.3
Organism Danio rerio(Zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157530.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGTTAACG AAGAGGCTGA AGTGTCCAAC CTTGACCATG TAGTTCTGGA TGAGGATCAC 
TCGGGTTCAA GGAGGTTGCA GAATCTCTGG AGAGATTCTC GTGAGGAAGC TCTGGGGGAG 
TATTACATGA GGAAATATGC CAAGTCTTCA GGAGGCGAAC ACTTCTATGG TGGGTCTGAG 
GATCTTTCTG ATGACATCAC ACAGCAACAA TTGCTTCCTG GGGTCAAGGA TCCTAATTTG 
TGGACTGTTA AGTGTAAGAT TGGAGAGGAG AGAGCAACTG CAATTTCCTT GATGCGAAAA 
TTCGTTGCTT ATCAGTGCAC TGACACGCCA CTTCAAATCA AATCAGTAGT TGCCCCTGAA 
CATGTCAAAG GTTATATCTA TGTGGAGGCG TACAAGCAGA CCCATGTCAA GGCTGCCATT 
GAGGGTGTGG GAAACCTGCG CATGGGCTTC TGGAACCAGC AGATGGTGCC CATTAAAGAG 
ATGACAGATG TGCTGAAAGT GGTTAAGGAA GTGACTAACC TTAAACCCAA GTCTTGGGTT 
CGTCTCAAGA GAGGCCTTTA TAAAGATGAC ATTGCCCAGG TGGACTATGT TGAACCTAGT 
CAGAACACGA TTTCGTTGAA AATGATTCCT CGTATTGACC TGGATCGTAT CAAAGCAAGG 
ATGAGTATGA AAGACTGGTT TGCAAAGAGA AAGAAGTTCA AGAGGCCCCC ACAGAGGCTT 
TTTGATGCTG AAAAGATCAG GTCTCTTGGT GGTGAGGTCA GCCATGATGG TGATTTCATG 
ATATTTGAAG CGAACCGCTA CAGCAGAAAG GGTTTCCTGT TCAAAAGCTT TGCCATGTCT 
GCTGTGATCA CAGAAGGGGT GAAGCCCACT CTGTCCGAGC TGGAGAAGTT TGAAGACCAG 
CCAGAGGGCA TTGACCTTGA AGTAGTCACA GAAACAACAG GTAAGGAGCG AGAACACAAT 
CTCCAGGCGG GAGATAATGT GGAGGTGTGT GAAGGGGAGT TGATCAACCT TCAGGGCAAA 
ATCCTGAGTG TGGATGGCAA CAAAATCACC ATCATGCCCA AGCACGAGGA CCTCAAGGAT 
CCTCTTGAGT TTCCAGCTCA TGAGTTGCGG AAGTATTTTC GTATGGGTGA CCATGTGAAG 
GTCATCGCGG GGCGTTATGA AGGGGATACA GGCCTCATAG TTCGCGTGGA GGAGAACTTT 
GTCATTCTGT TCTCTGACCT CACCATGCAC GAGTTGAAAG TTTTGCCCCG GGACCTGCAG 
CTGTGCTCTG AGACGGCTTC AGGTGTGGAT GCTGGAGGCC AGCATGAGTG GGGGGAGCTG 
GTACAGTTAG ACCCTCAAAC TGTAGGTGTC ATTGTCAGGC TGGAGAGAGA GACCTTTCAG 
GTTCTGAACA TGCATGGGAA AGTTTTAACA GTACGGCACC AGGCAGTGAA CAGGCGGAAG 
GACAACCGGT TTGCTGTCGC TCTCGACTCT GAGCAAAACA ACATCCATGT TAAGGATATT 
GTGAAAGTCA TTGATGGACC TCATTCGGGC CGTGAAGGTG AAATTCGCCA CATCTTCAGA 
GGCTTTGCTT TTCTTCACTG CAAGAAGCTG GTTGAAAACG GAGGCATGTT TGTTTGTAAA 
GCACGTCACC TTGTACTGGC AGGTGGCTCT AAGCCCAGAG ATGTGACCAA TTTTACAGTG 
GGTGGCTTTG CTCCCATGAG CCCACGCATC AGCAGCCCAA TGCACCCTGG AGGAGGTGGT 
CAGCCGCAAA GAGGTGGAGG TGGTGGTGGT GGTGGTGGCA TGGGCCGAGG CCGTGGACGA 
CGAGACAATG ATCTCATTGG CCAGACTGTT CGTATATCCC AGGGACCATA CAAAGGCTAC 
ATTGGTGTGG TGAAGGATGC CACAGAATCC ACCGCTAGGG TAGAGCTCCA CTCAACCTGC 
CAGACCATCT CTGTGGATCG CCAGCGCCTT ACAACTGTTG GTGGCAAAGA AAGACAAGGA 
AGAAGTTCCA CCCATCTACG AACCCCGATG TATGGTTCCC AGACACCCAT TTATGGGACT 
GGCTCACGTA CTCCCATGTA TGGTAGCCAG ACACCCCTCC ATGATGGAAG TCGTACTCCT 
CACTATGGCT CACAGACTCC TCTTCATGAT GGGAGCAGGA CACCTGGCCA AAGTGGTGCG 
TGGGACCCCA ACAATCCAAA CACACCATCA CGGCCTGATG ACGAGTATGA GTTTGCCTAC 
GATGACGAAC CCTCGCCGTC TCCTCAAGGC TATGGTGGGA CACCCAACCC TCAGACACCT 
GGATATCCAG AAGTGCCCTC TCCGCAGGTC AACCCTCAGT ATAACCCACA GACTCCTGGG 
ACTCCTGCCA TGTACAACAC AGAGCAATAC TCCCCATATG CAGCACCTTC ACCCCAGGGC 
TCCTACCAGC CCAGTCCAAG TCCACAGAGT TACCACCAAG TGGCGCCAAG CCCGGTGGGA 
TACCAAAACA CACACTCGCC TGCCAGTTAC CACCCCACTC CATCACCCAT GGCTTACCAG 
GCCAGTCCAA GTCCCAGTCC GGTAGGGTAT AGTCCCATGA CTCCTGGCGC TCCGTCTCCT 
GGGGGTTACA ACCCTCACAC CCCTGGCTCC AACATTGACC AAGCCTCCAA TGACTGGGTC 
ACCACAGACA TCATGGTGCG TGTGAAAGAC ACATTCCTTG ATGGAGGCGT CATCAATCAG 
ACCGGCATCA TCCGCAGTGT CACCGGAGGA ATGTGCTCTG TTTTTCTGCA AGACACTGAA 
AAGGTTGTTA GCATTTCAAG CGAACACTTG GAGCCTGTTA CACCAACCAA AAACAATAAG 
GTGAAGGTGA TTCTGGGAGA GGACAGGGAG GCCACAGGAG TCCTGCTTAG TATCGATGGT 
GAAGACGGCA TTGTTAGAAT GGAGTTGGAC GAGCAGCTGA AGATTCTCAA TCTCCGCTTC 
CTTGGGAAAC TGGAAGTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa38948
Clone ID Related Accession (Same CDS sequence) XM_021481463.1 , NM_131673.2 , XM_017351012.1
Accession Version XM_021481463.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product transcription elongation factor SPT5 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGAAGAGG CTGAAGTGTC CAACCTTGAC CATGTAGTTC TGGATGAGGA TCACTCGGGT 
TCAAGGAGGT TGCAGAATCT CTGGAGAGAT TCTCGTGAGG AAGCTCTGGG GGAGTATTAC 
ATGAGGAAAT ATGCCAAGTC TTCAGGAGGC GAACACTTCT ATGGTGGGTC TGAGGATCTT 
TCTGATGACA TCACACAGCA ACAATTGCTT CCTGGGGTCA AGGATCCTAA TTTGTGGACT 
GTTAAGTGTA AGATTGGAGA GGAGAGAGCA ACTGCAATTT CCTTGATGCG AAAATTCGTT 
GCTTATCAGT GCACTGACAC GCCACTTCAA ATCAAATCAG TAGTTGCCCC TGAACATGTC 
AAAGGTTATA TCTATGTGGA GGCGTACAAG CAGACCCATG TCAAGGCTGC CATTGAGGGT 
GTGGGAAACC TGCGCATGGG CTTCTGGAAC CAGCAGATGG TGCCCATTAA AGAGATGACA 
GATGTGCTGA AAGTGGTTAA GGAAGTGACT AACCTTAAAC CCAAGTCTTG GGTTCGTCTC 
AAGAGAGGCC TTTATAAAGA TGACATTGCC CAGGTGGACT ATGTTGAACC TAGTCAGAAC 
ACGATTTCGT TGAAAATGAT TCCTCGTATT GACCTGGATC GTATCAAAGC AAGGATGAGT 
ATGAAAGACT GGTTTGCAAA GAGAAAGAAG TTCAAGAGGC CCCCACAGAG GCTTTTTGAT 
GCTGAAAAGA TCAGGTCTCT TGGTGGTGAG GTCAGCCATG ATGGTGATTT CATGATATTT 
GAAGCGAACC GCTACAGCAG AAAGGGTTTC CTGTTCAAAA GCTTTGCCAT GTCTGCTGTG 
ATCACAGAAG GGGTGAAGCC CACTCTGTCC GAGCTGGAGA AGTTTGAAGA CCAGCCAGAG 
GGCATTGACC TTGAAGTAGT CACAGAAACA ACAGGTAAGG AGCGAGAACA CAATCTCCAG 
GCGGGAGATA ATGTGGAGGT GTGTGAAGGG GAGTTGATCA ACCTTCAGGG CAAAATCCTG 
AGTGTGGATG GCAACAAAAT CACCATCATG CCCAAGCACG AGGACCTCAA GGATCCTCTT 
GAGTTTCCAG CTCATGAGTT GCGGAAGTAT TTTCGTATGG GTGACCATGT GAAGGTCATC 
GCGGGGCGTT ATGAAGGGGA TACAGGCCTC ATAGTTCGCG TGGAGGAGAA CTTTGTCATT 
CTGTTCTCTG ACCTCACCAT GCACGAGTTG AAAGTTTTGC CCCGGGACCT GCAGCTGTGC 
TCTGAGACGG CTTCAGGTGT GGATGCTGGA GGCCAGCATG AGTGGGGGGA GCTGGTACAG 
TTAGACCCTC AAACTGTAGG TGTCATTGTC AGGCTGGAGA GAGAGACCTT TCAGGTTCTG 
AACATGCATG GGAAAGTTTT AACAGTACGG CACCAGGCAG TGAACAGGCG GAAGGACAAC 
CGGTTTGCTG TCGCTCTCGA CTCTGAGCAA AACAACATCC ATGTTAAGGA TATTGTGAAA 
GTCATTGATG GACCTCATTC GGGCCGTGAA GGTGAAATTC GCCACATCTT CAGAGGCTTT 
GCTTTTCTTC ACTGCAAGAA GCTGGTTGAA AACGGAGGCA TGTTTGTTTG TAAAGCACGT 
CACCTTGTAC TGGCAGGTGG CTCTAAGCCC AGAGATGTGA CCAATTTTAC AGTGGGTGGC 
TTTGCTCCCA TGAGCCCACG CATCAGCAGC CCAATGCACC CTGGAGGAGG TGGTCAGCCG 
CAAAGAGGTG GAGGTGGTGG TGGTGGTGGT GGCATGGGCC GAGGCCGTGG ACGACGAGAC 
AATGATCTCA TTGGCCAGAC TGTTCGTATA TCCCAGGGAC CATACAAAGG CTACATTGGT 
GTGGTGAAGG ATGCCACAGA ATCCACCGCT AGGGTAGAGC TCCACTCAAC CTGCCAGACC 
ATCTCTGTGG ATCGCCAGCG CCTTACAACT GTTGGTGGCA AAGAAAGACA AGGAAGAAGT 
TCCACCCATC TACGAACCCC GATGTATGGT TCCCAGACAC CCATTTATGG GACTGGCTCA 
CGTACTCCCA TGTATGGTAG CCAGACACCC CTCCATGATG GAAGTCGTAC TCCTCACTAT 
GGCTCACAGA CTCCTCTTCA TGATGGGAGC AGGACACCTG GCCAAAGTGG TGCGTGGGAC 
CCCAACAATC CAAACACACC ATCACGGCCT GATGACGAGT ATGAGTTTGC CTACGATGAC 
GAACCCTCGC CGTCTCCTCA AGGCTATGGT GGGACACCCA ACCCTCAGAC ACCTGGATAT 
CCAGAAGTGC CCTCTCCGCA GGTCAACCCT CAGTATAACC CACAGACTCC TGGGACTCCT 
GCCATGTACA ACACAGAGCA ATACTCCCCA TATGCAGCAC CTTCACCCCA GGGCTCCTAC 
CAGCCCAGTC CAAGTCCACA GAGTTACCAC CAAGTGGCGC CAAGCCCGGT GGGATACCAA 
AACACACACT CGCCTGCCAG TTACCACCCC ACTCCATCAC CCATGGCTTA CCAGGCCAGT 
CCAAGTCCCA GTCCGGTAGG GTATAGTCCC ATGACTCCTG GCGCTCCGTC TCCTGGGGGT 
TACAACCCTC ACACCCCTGG CTCCAACATT GACCAAGCCT CCAATGACTG GGTCACCACA 
GACATCATGG TGCGTGTGAA AGACACATTC CTTGATGGAG GCGTCATCAA TCAGACCGGC 
ATCATCCGCA GTGTCACCGG AGGAATGTGC TCTGTTTTTC TGCAAGACAC TGAAAAGGTT 
GTTAGCATTT CAAGCGAACA CTTGGAGCCT GTTACACCAA CCAAAAACAA TAAGGTGAAG 
GTGATTCTGG GAGAGGACAG GGAGGCCACA GGAGTCCTGC TTAGTATCGA TGGTGAAGAC 
GGCATTGTTA GAATGGAGTT GGACGAGCAG CTGAAGATTC TCAATCTCCG CTTCCTTGGG 
AAACTGGAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_021337138.1
CDS105..3359
Translation

Target ORF information:

RefSeq Version XM_021481463.1
Organism Danio rerio(zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_021481463.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGAAGAGG CTGAAGTGTC CAACCTTGAC CATGTAGTTC TGGATGAGGA TCACTCGGGT 
TCAAGGAGGT TGCAGAATCT CTGGAGAGAT TCTCGTGAGG AAGCTCTGGG GGAGTATTAC 
ATGAGGAAAT ATGCCAAGTC TTCAGGAGGC GAACACTTCT ATGGTGGGTC TGAGGATCTT 
TCTGATGACA TCACACAGCA ACAATTGCTT CCTGGGGTCA AGGATCCTAA TTTGTGGACT 
GTTAAGTGTA AGATTGGAGA GGAGAGAGCA ACTGCAATTT CCTTGATGCG AAAATTCGTT 
GCTTATCAGT GCACTGACAC GCCACTTCAA ATCAAATCAG TAGTTGCCCC TGAACATGTC 
AAAGGTTATA TCTATGTGGA GGCGTACAAG CAGACCCATG TCAAGGCTGC CATTGAGGGT 
GTGGGAAACC TGCGCATGGG CTTCTGGAAC CAGCAGATGG TGCCCATTAA AGAGATGACA 
GATGTGCTGA AAGTGGTTAA GGAAGTGACT AACCTTAAAC CCAAGTCTTG GGTTCGTCTC 
AAGAGAGGCC TTTATAAAGA TGACATTGCC CAGGTGGACT ATGTTGAACC TAGTCAGAAC 
ACGATTTCGT TGAAAATGAT TCCTCGTATT GACCTGGATC GTATCAAAGC AAGGATGAGT 
ATGAAAGACT GGTTTGCAAA GAGAAAGAAG TTCAAGAGGC CCCCACAGAG GCTTTTTGAT 
GCTGAAAAGA TCAGGTCTCT TGGTGGTGAG GTCAGCCATG ATGGTGATTT CATGATATTT 
GAAGCGAACC GCTACAGCAG AAAGGGTTTC CTGTTCAAAA GCTTTGCCAT GTCTGCTGTG 
ATCACAGAAG GGGTGAAGCC CACTCTGTCC GAGCTGGAGA AGTTTGAAGA CCAGCCAGAG 
GGCATTGACC TTGAAGTAGT CACAGAAACA ACAGGTAAGG AGCGAGAACA CAATCTCCAG 
GCGGGAGATA ATGTGGAGGT GTGTGAAGGG GAGTTGATCA ACCTTCAGGG CAAAATCCTG 
AGTGTGGATG GCAACAAAAT CACCATCATG CCCAAGCACG AGGACCTCAA GGATCCTCTT 
GAGTTTCCAG CTCATGAGTT GCGGAAGTAT TTTCGTATGG GTGACCATGT GAAGGTCATC 
GCGGGGCGTT ATGAAGGGGA TACAGGCCTC ATAGTTCGCG TGGAGGAGAA CTTTGTCATT 
CTGTTCTCTG ACCTCACCAT GCACGAGTTG AAAGTTTTGC CCCGGGACCT GCAGCTGTGC 
TCTGAGACGG CTTCAGGTGT GGATGCTGGA GGCCAGCATG AGTGGGGGGA GCTGGTACAG 
TTAGACCCTC AAACTGTAGG TGTCATTGTC AGGCTGGAGA GAGAGACCTT TCAGGTTCTG 
AACATGCATG GGAAAGTTTT AACAGTACGG CACCAGGCAG TGAACAGGCG GAAGGACAAC 
CGGTTTGCTG TCGCTCTCGA CTCTGAGCAA AACAACATCC ATGTTAAGGA TATTGTGAAA 
GTCATTGATG GACCTCATTC GGGCCGTGAA GGTGAAATTC GCCACATCTT CAGAGGCTTT 
GCTTTTCTTC ACTGCAAGAA GCTGGTTGAA AACGGAGGCA TGTTTGTTTG TAAAGCACGT 
CACCTTGTAC TGGCAGGTGG CTCTAAGCCC AGAGATGTGA CCAATTTTAC AGTGGGTGGC 
TTTGCTCCCA TGAGCCCACG CATCAGCAGC CCAATGCACC CTGGAGGAGG TGGTCAGCCG 
CAAAGAGGTG GAGGTGGTGG TGGTGGTGGT GGCATGGGCC GAGGCCGTGG ACGACGAGAC 
AATGATCTCA TTGGCCAGAC TGTTCGTATA TCCCAGGGAC CATACAAAGG CTACATTGGT 
GTGGTGAAGG ATGCCACAGA ATCCACCGCT AGGGTAGAGC TCCACTCAAC CTGCCAGACC 
ATCTCTGTGG ATCGCCAGCG CCTTACAACT GTTGGTGGCA AAGAAAGACA AGGAAGAAGT 
TCCACCCATC TACGAACCCC GATGTATGGT TCCCAGACAC CCATTTATGG GACTGGCTCA 
CGTACTCCCA TGTATGGTAG CCAGACACCC CTCCATGATG GAAGTCGTAC TCCTCACTAT 
GGCTCACAGA CTCCTCTTCA TGATGGGAGC AGGACACCTG GCCAAAGTGG TGCGTGGGAC 
CCCAACAATC CAAACACACC ATCACGGCCT GATGACGAGT ATGAGTTTGC CTACGATGAC 
GAACCCTCGC CGTCTCCTCA AGGCTATGGT GGGACACCCA ACCCTCAGAC ACCTGGATAT 
CCAGAAGTGC CCTCTCCGCA GGTCAACCCT CAGTATAACC CACAGACTCC TGGGACTCCT 
GCCATGTACA ACACAGAGCA ATACTCCCCA TATGCAGCAC CTTCACCCCA GGGCTCCTAC 
CAGCCCAGTC CAAGTCCACA GAGTTACCAC CAAGTGGCGC CAAGCCCGGT GGGATACCAA 
AACACACACT CGCCTGCCAG TTACCACCCC ACTCCATCAC CCATGGCTTA CCAGGCCAGT 
CCAAGTCCCA GTCCGGTAGG GTATAGTCCC ATGACTCCTG GCGCTCCGTC TCCTGGGGGT 
TACAACCCTC ACACCCCTGG CTCCAACATT GACCAAGCCT CCAATGACTG GGTCACCACA 
GACATCATGG TGCGTGTGAA AGACACATTC CTTGATGGAG GCGTCATCAA TCAGACCGGC 
ATCATCCGCA GTGTCACCGG AGGAATGTGC TCTGTTTTTC TGCAAGACAC TGAAAAGGTT 
GTTAGCATTT CAAGCGAACA CTTGGAGCCT GTTACACCAA CCAAAAACAA TAAGGTGAAG 
GTGATTCTGG GAGAGGACAG GGAGGCCACA GGAGTCCTGC TTAGTATCGA TGGTGAAGAC 
GGCATTGTTA GAATGGAGTT GGACGAGCAG CTGAAGATTC TCAATCTCCG CTTCCTTGGG 
AAACTGGAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa38948
Clone ID Related Accession (Same CDS sequence) XM_021481463.1 , NM_131673.2 , XM_017351012.1
Accession Version XM_017351012.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product transcription elongation factor SPT5 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGAAGAGG CTGAAGTGTC CAACCTTGAC CATGTAGTTC TGGATGAGGA TCACTCGGGT 
TCAAGGAGGT TGCAGAATCT CTGGAGAGAT TCTCGTGAGG AAGCTCTGGG GGAGTATTAC 
ATGAGGAAAT ATGCCAAGTC TTCAGGAGGC GAACACTTCT ATGGTGGGTC TGAGGATCTT 
TCTGATGACA TCACACAGCA ACAATTGCTT CCTGGGGTCA AGGATCCTAA TTTGTGGACT 
GTTAAGTGTA AGATTGGAGA GGAGAGAGCA ACTGCAATTT CCTTGATGCG AAAATTCGTT 
GCTTATCAGT GCACTGACAC GCCACTTCAA ATCAAATCAG TAGTTGCCCC TGAACATGTC 
AAAGGTTATA TCTATGTGGA GGCGTACAAG CAGACCCATG TCAAGGCTGC CATTGAGGGT 
GTGGGAAACC TGCGCATGGG CTTCTGGAAC CAGCAGATGG TGCCCATTAA AGAGATGACA 
GATGTGCTGA AAGTGGTTAA GGAAGTGACT AACCTTAAAC CCAAGTCTTG GGTTCGTCTC 
AAGAGAGGCC TTTATAAAGA TGACATTGCC CAGGTGGACT ATGTTGAACC TAGTCAGAAC 
ACGATTTCGT TGAAAATGAT TCCTCGTATT GACCTGGATC GTATCAAAGC AAGGATGAGT 
ATGAAAGACT GGTTTGCAAA GAGAAAGAAG TTCAAGAGGC CCCCACAGAG GCTTTTTGAT 
GCTGAAAAGA TCAGGTCTCT TGGTGGTGAG GTCAGCCATG ATGGTGATTT CATGATATTT 
GAAGCGAACC GCTACAGCAG AAAGGGTTTC CTGTTCAAAA GCTTTGCCAT GTCTGCTGTG 
ATCACAGAAG GGGTGAAGCC CACTCTGTCC GAGCTGGAGA AGTTTGAAGA CCAGCCAGAG 
GGCATTGACC TTGAAGTAGT CACAGAAACA ACAGGTAAGG AGCGAGAACA CAATCTCCAG 
GCGGGAGATA ATGTGGAGGT GTGTGAAGGG GAGTTGATCA ACCTTCAGGG CAAAATCCTG 
AGTGTGGATG GCAACAAAAT CACCATCATG CCCAAGCACG AGGACCTCAA GGATCCTCTT 
GAGTTTCCAG CTCATGAGTT GCGGAAGTAT TTTCGTATGG GTGACCATGT GAAGGTCATC 
GCGGGGCGTT ATGAAGGGGA TACAGGCCTC ATAGTTCGCG TGGAGGAGAA CTTTGTCATT 
CTGTTCTCTG ACCTCACCAT GCACGAGTTG AAAGTTTTGC CCCGGGACCT GCAGCTGTGC 
TCTGAGACGG CTTCAGGTGT GGATGCTGGA GGCCAGCATG AGTGGGGGGA GCTGGTACAG 
TTAGACCCTC AAACTGTAGG TGTCATTGTC AGGCTGGAGA GAGAGACCTT TCAGGTTCTG 
AACATGCATG GGAAAGTTTT AACAGTACGG CACCAGGCAG TGAACAGGCG GAAGGACAAC 
CGGTTTGCTG TCGCTCTCGA CTCTGAGCAA AACAACATCC ATGTTAAGGA TATTGTGAAA 
GTCATTGATG GACCTCATTC GGGCCGTGAA GGTGAAATTC GCCACATCTT CAGAGGCTTT 
GCTTTTCTTC ACTGCAAGAA GCTGGTTGAA AACGGAGGCA TGTTTGTTTG TAAAGCACGT 
CACCTTGTAC TGGCAGGTGG CTCTAAGCCC AGAGATGTGA CCAATTTTAC AGTGGGTGGC 
TTTGCTCCCA TGAGCCCACG CATCAGCAGC CCAATGCACC CTGGAGGAGG TGGTCAGCCG 
CAAAGAGGTG GAGGTGGTGG TGGTGGTGGT GGCATGGGCC GAGGCCGTGG ACGACGAGAC 
AATGATCTCA TTGGCCAGAC TGTTCGTATA TCCCAGGGAC CATACAAAGG CTACATTGGT 
GTGGTGAAGG ATGCCACAGA ATCCACCGCT AGGGTAGAGC TCCACTCAAC CTGCCAGACC 
ATCTCTGTGG ATCGCCAGCG CCTTACAACT GTTGGTGGCA AAGAAAGACA AGGAAGAAGT 
TCCACCCATC TACGAACCCC GATGTATGGT TCCCAGACAC CCATTTATGG GACTGGCTCA 
CGTACTCCCA TGTATGGTAG CCAGACACCC CTCCATGATG GAAGTCGTAC TCCTCACTAT 
GGCTCACAGA CTCCTCTTCA TGATGGGAGC AGGACACCTG GCCAAAGTGG TGCGTGGGAC 
CCCAACAATC CAAACACACC ATCACGGCCT GATGACGAGT ATGAGTTTGC CTACGATGAC 
GAACCCTCGC CGTCTCCTCA AGGCTATGGT GGGACACCCA ACCCTCAGAC ACCTGGATAT 
CCAGAAGTGC CCTCTCCGCA GGTCAACCCT CAGTATAACC CACAGACTCC TGGGACTCCT 
GCCATGTACA ACACAGAGCA ATACTCCCCA TATGCAGCAC CTTCACCCCA GGGCTCCTAC 
CAGCCCAGTC CAAGTCCACA GAGTTACCAC CAAGTGGCGC CAAGCCCGGT GGGATACCAA 
AACACACACT CGCCTGCCAG TTACCACCCC ACTCCATCAC CCATGGCTTA CCAGGCCAGT 
CCAAGTCCCA GTCCGGTAGG GTATAGTCCC ATGACTCCTG GCGCTCCGTC TCCTGGGGGT 
TACAACCCTC ACACCCCTGG CTCCAACATT GACCAAGCCT CCAATGACTG GGTCACCACA 
GACATCATGG TGCGTGTGAA AGACACATTC CTTGATGGAG GCGTCATCAA TCAGACCGGC 
ATCATCCGCA GTGTCACCGG AGGAATGTGC TCTGTTTTTC TGCAAGACAC TGAAAAGGTT 
GTTAGCATTT CAAGCGAACA CTTGGAGCCT GTTACACCAA CCAAAAACAA TAAGGTGAAG 
GTGATTCTGG GAGAGGACAG GGAGGCCACA GGAGTCCTGC TTAGTATCGA TGGTGAAGAC 
GGCATTGTTA GAATGGAGTT GGACGAGCAG CTGAAGATTC TCAATCTCCG CTTCCTTGGG 
AAACTGGAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017206501.1
CDS157..3411
Translation

Target ORF information:

RefSeq Version XM_017351012.1
Organism Danio rerio(Zebrafish)
Definition Danio rerio SPT5 homolog, DSIF elongation factor subunit (supt5h), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017351012.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCAGACA GCGAGGACAG TGATTTCTCT GATAATCAGA GTGAGAGGAG CAGCGAGGCT 
GAAGAGGTGG AGGAGAATGA GGAAGAGGAA GAACAGGGAA GTGTAGCCGG CAGTGACAAG 
GCTGAGGAAG AGGGAGAGGA TTTGGAAGAT GAGGAGGAAT ATGATGAAGA GGAAGAGGAG 
GATGACGACC GACCTCGGAA GAAAGCTCGG CATGGTGGCT TCATCTTGGA TGAAGCCGAT 
GTTGATGATG AGTATGAAGA TGAAGATCCA TGGGAGGATG GTGCTGAAGA CATCTTAGAA 
AAAGAAGAGG CTGAAGTGTC CAACCTTGAC CATGTAGTTC TGGATGAGGA TCACTCGGGT 
TCAAGGAGGT TGCAGAATCT CTGGAGAGAT TCTCGTGAGG AAGCTCTGGG GGAGTATTAC 
ATGAGGAAAT ATGCCAAGTC TTCAGGAGGC GAACACTTCT ATGGTGGGTC TGAGGATCTT 
TCTGATGACA TCACACAGCA ACAATTGCTT CCTGGGGTCA AGGATCCTAA TTTGTGGACT 
GTTAAGTGTA AGATTGGAGA GGAGAGAGCA ACTGCAATTT CCTTGATGCG AAAATTCGTT 
GCTTATCAGT GCACTGACAC GCCACTTCAA ATCAAATCAG TAGTTGCCCC TGAACATGTC 
AAAGGTTATA TCTATGTGGA GGCGTACAAG CAGACCCATG TCAAGGCTGC CATTGAGGGT 
GTGGGAAACC TGCGCATGGG CTTCTGGAAC CAGCAGATGG TGCCCATTAA AGAGATGACA 
GATGTGCTGA AAGTGGTTAA GGAAGTGACT AACCTTAAAC CCAAGTCTTG GGTTCGTCTC 
AAGAGAGGCC TTTATAAAGA TGACATTGCC CAGGTGGACT ATGTTGAACC TAGTCAGAAC 
ACGATTTCGT TGAAAATGAT TCCTCGTATT GACCTGGATC GTATCAAAGC AAGGATGAGT 
ATGAAAGACT GGTTTGCAAA GAGAAAGAAG TTCAAGAGGC CCCCACAGAG GCTTTTTGAT 
GCTGAAAAGA TCAGGTCTCT TGGTGGTGAG GTCAGCCATG ATGGTGATTT CATGATATTT 
GAAGCGAACC GCTACAGCAG AAAGGGTTTC CTGTTCAAAA GCTTTGCCAT GTCTGCTGTG 
ATCACAGAAG GGGTGAAGCC CACTCTGTCC GAGCTGGAGA AGTTTGAAGA CCAGCCAGAG 
GGCATTGACC TTGAAGTAGT CACAGAAACA ACAGGTAAGG AGCGAGAACA CAATCTCCAG 
GCGGGAGATA ATGTGGAGGT GTGTGAAGGG GAGTTGATCA ACCTTCAGGG CAAAATCCTG 
AGTGTGGATG GCAACAAAAT CACCATCATG CCCAAGCACG AGGACCTCAA GGATCCTCTT 
GAGTTTCCAG CTCATGAGTT GCGGAAGTAT TTTCGTATGG GTGACCATGT GAAGGTCATC 
GCGGGGCGTT ATGAAGGGGA TACAGGCCTC ATAGTTCGCG TGGAGGAGAA CTTTGTCATT 
CTGTTCTCTG ACCTCACCAT GCACGAGTTG AAAGTTTTGC CCCGGGACCT GCAGCTGTGC 
TCTGAGACGG CTTCAGGTGT GGATGCTGGA GGCCAGCATG AGTGGGGGGA GCTGGTACAG 
TTAGACCCTC AAACTGTAGG TGTCATTGTC AGGCTGGAGA GAGAGACCTT TCAGGTTCTG 
AACATGCATG GGAAAGTTTT AACAGTACGG CACCAGGCAG TGAACAGGCG GAAGGACAAC 
CGGTTTGCTG TCGCTCTCGA CTCTGAGCAA AACAACATCC ATGTTAAGGA TATTGTGAAA 
GTCATTGATG GACCTCATTC GGGCCGTGAA GGTGAAATTC GCCACATCTT CAGAGGCTTT 
GCTTTTCTTC ACTGCAAGAA GCTGGTTGAA AACGGAGGCA TGTTTGTTTG TAAAGCACGT 
CACCTTGTAC TGGCAGGTGG CTCTAAGCCC AGAGATGTGA CCAATTTTAC AGTGGGTGGC 
TTTGCTCCCA TGAGCCCACG CATCAGCAGC CCAATGCACC CTGGAGGAGG TGGTCAGCCG 
CAAAGAGGTG GAGGTGGTGG TGGTGGTGGT GGCATGGGCC GAGGCCGTGG ACGACGAGAC 
AATGATCTCA TTGGCCAGAC TGTTCGTATA TCCCAGGGAC CATACAAAGG CTACATTGGT 
GTGGTGAAGG ATGCCACAGA ATCCACCGCT AGGGTAGAGC TCCACTCAAC CTGCCAGACC 
ATCTCTGTGG ATCGCCAGCG CCTTACAACT GTTGGTGGCA AAGAAAGACA AGGAAGAAGT 
TCCACCCATC TACGAACCCC GATGTATGGT TCCCAGACAC CCATTTATGG GACTGGCTCA 
CGTACTCCCA TGTATGGTAG CCAGACACCC CTCCATGATG GAAGTCGTAC TCCTCACTAT 
GGCTCACAGA CTCCTCTTCA TGATGGGAGC AGGACACCTG GCCAAAGTGG TGCGTGGGAC 
CCCAACAATC CAAACACACC ATCACGGCCT GATGACGAGT ATGAGTTTGC CTACGATGAC 
GAACCCTCGC CGTCTCCTCA AGGCTATGGT GGGACACCCA ACCCTCAGAC ACCTGGATAT 
CCAGAAGTGC CCTCTCCGCA GGTCAACCCT CAGTATAACC CACAGACTCC TGGGACTCCT 
GCCATGTACA ACACAGAGCA ATACTCCCCA TATGCAGCAC CTTCACCCCA GGGCTCCTAC 
CAGCCCAGTC CAAGTCCACA GAGTTACCAC CAAGTGGCGC CAAGCCCGGT GGGATACCAA 
AACACACACT CGCCTGCCAG TTACCACCCC ACTCCATCAC CCATGGCTTA CCAGGCCAGT 
CCAAGTCCCA GTCCGGTAGG GTATAGTCCC ATGACTCCTG GCGCTCCGTC TCCTGGGGGT 
TACAACCCTC ACACCCCTGG CTCCAACATT GACCAAGCCT CCAATGACTG GGTCACCACA 
GACATCATGG TGCGTGTGAA AGACACATTC CTTGATGGAG GCGTCATCAA TCAGACCGGC 
ATCATCCGCA GTGTCACCGG AGGAATGTGC TCTGTTTTTC TGCAAGACAC TGAAAAGGTT 
GTTAGCATTT CAAGCGAACA CTTGGAGCCT GTTACACCAA CCAAAAACAA TAAGGTGAAG 
GTGATTCTGG GAGAGGACAG GGAGGCCACA GGAGTCCTGC TTAGTATCGA TGGTGAAGAC 
GGCATTGTTA GAATGGAGTT GGACGAGCAG CTGAAGATTC TCAATCTCCG CTTCCTTGGG 
AAACTGGAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Maternal methylmercury from a wild-caught walleye diet induces developmental abnormalities in zebrafish.
Reproductive toxicology (Elmsford, N.Y.)65272-282(2016 10)
Liu Q,Klingler RH,Wimpee B,Dellinger M,King-Heiden T,Grzybowski J,Gerstenberger SL,Weber DN,Carvan MJ 3rd


book

RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Blood128(13)1701-10(2016 09)
Yang Q,Liu X,Zhou T,Cook J,Nguyen K,Bai X


book

Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation.
Genes to cells : devoted to molecular & cellular mechanisms16(2)231-42(2011 Feb)
Taneda T,Zhu W,Cao Q,Watanabe H,Yamaguchi Y,Handa H,Wada T


book

TIF1gamma controls erythroid cell fate by regulating transcription elongation.
Cell142(1)133-43(2010 Jul)
Bai X,Kim J,Yang Z,Jurynec MJ,Akie TE,Lee J,LeBlanc J,Sessa A,Jiang H,DiBiase A,Zhou Y,Grunwald DJ,Lin S,Cantor AB,Orkin SH,Zon LI


book

Repression of RNA polymerase II elongation in vivo is critically dependent on the C-terminus of Spt5.
PloS one4(9)e6918(2009 Sep)
Chen H,Contreras X,Yamaguchi Y,Handa H,Peterlin BM,Guo S