pan2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pan2
Entrez Gene ID	792831
Full Name	PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
Synonyms	fc27e10,si:dkey-126g1.10,wu:fc27e10
General protein information	Preferred Names PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) Names PAB-dependent poly(A)-specific ribonuclease subunit PAN2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001919965.6	XP_001920000.4	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X2
XM_005162345.2	XP_005162402.1	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X1
XM_001919965.5	XP_001920000.4	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X2
XM_005162345.3	XP_005162402.1	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pan2	NP_001008862.1
Kluyveromyces lactis	KLLA0E18877g	XP_454804.1
Magnaporthe oryzae (rice blast fungus)	MGG_17449	XP_003718602.1
Neurospora crassa	NCU10733	XP_001728299.1
Pan troglodytes (chimpanzee)	LOC451995	XP_003952222.1
Saccharomyces cerevisiae (baker's yeast)	PAN2	NP_011421.1
	PAN2	XP_001114990.2
Canis lupus familiaris (dog)	PAN2	XP_531635.3
Danio rerio (zebrafish)	LOC792831	XP_001920000.4
Homo sapiens (human)	PAN2	NP_001159751.1
Bos taurus (cattle)	PAN2	NP_001073078.2
Gallus gallus (chicken)	PAN2	NP_001012972.1
Drosophila melanogaster (fruit fly)	CG8232	NP_610427.2
Schizosaccharomyces pombe (fission yeast)	ubp13	NP_593053.2
Xenopus tropicalis (tropical clawed frog)	pan2	XP_002934576.2
Mus musculus (house mouse)	Pan2	NP_598753.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010626	XP_310122.4
Eremothecium gossypii	AGOS_AFL055W	NP_985493.2

Gene Ontology

The following pan2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pan2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27930	XM_005162345.3 Latest version!	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa27932	XM_001919965.6 Latest version!	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa27932	XM_001919965.5	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa27930	XM_005162345.2	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pan2 ( XM_005162345.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162402.1 Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27930
Clone ID Related Accession (Same CDS sequence)	XM_005162345.2 , XM_005162345.3
Accession Version	XM_005162345.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGTAGCC CAAAAAGCAC AGCCGTATTT 
CCCTCAGTGA TGGGTTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162402.1
CDS	92..3712
Translation	MMNFEGLDPGLGDYAPGLHGGLEPGLDSSLDPRLDPALLQGVEIDPDGLAVSESVHIVEGMYSELHSVAAEVGIPVTATHFDLQEELLWMGNHRGHASSFFGPTMGRYSSFQVHMTDDIRHIQSMDTGVLFLTKNNLRCLTRGGFIMFDYPMEEGADMHSLILTENNTLLMGGLQNYVAELDLTTVQETQKFTVEVPGVAIMRQTSRFFFCGHTSGKVSLRDLRTFKMEHEFDAFSGSLSDFDIHGNLLAACGFSSRGLNGLSCDRFLMVYDLRMMRAVTPLQVHVDPLFLRFIPTYTSRLAIISQTGQCQFCEPTGLASLADIFHVNTVGQLLMSFDVSSSKQALSFGDSGGCVHLWSSAPEVSYNSYSRDTELALPCLVDSLPQMDWNHELMPLSLLPMPLTSTEPLLSDWPTALIAPSPRRAPPVDPEILRTMKTVGFIGYAPNPRTRPRNQVPYKIKDVELDYDSYNQVPESPIGRDEEPHLYMVPKKYRKVTIKYSKLGLEDFDFKHYNRTLFAGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILAQLHQETLEQEGPQAYRGASSSSVLSSSGESVISRLFGCEVENSSLCCCGKETVRSSLTLLFTMHYPEQSSLEKKIEEYEFVDILRKSICLEQSTQAWCDNCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKAQTEYVFNKALKKEAAEPPKPKEPLPTEWCLEEELCNVEGLTFDTQAEDMRHVWIPLSLRMCISKAQGLEVSSLTEGEELTESEEAEGVSIYDLFVTVSHVQDTRTGGNMVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKAESVQFDVNWKVPAVLYYIKRNYHTKFDLRIKNPIEASVLLTEASLARKQRKSHATFIPLMVSEMPQVGDLVGLDAEFVTLNQEEAELRSDGTKSTIKPSQMSVARITCVRGQGQNEGVPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDTGVRFVGHGLQKDFRVINLMVPKDQVIDTVYLFHMPRKRMISLRFLAWYFLDLNIQGETHDSIEDARTALQLYRKYLELSHNGGQEEFRKVLKALYEKGRKLDWKVPDIDSADGQGSPKSTAVFPSVMGL

Target ORF information:

RefSeq Version	XM_005162345.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162345.2

ORF Insert Sequence:

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGTAGCC CAAAAAGCAC AGCCGTATTT 
CCCTCAGTGA TGGGTTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pan2 ( XM_005162345.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162402.1 Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27930
Clone ID Related Accession (Same CDS sequence)	XM_005162345.2 , XM_005162345.3
Accession Version	XM_005162345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGTAGCC CAAAAAGCAC AGCCGTATTT 
CCCTCAGTGA TGGGTTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162402.1
CDS	90..3710
Translation	MMNFEGLDPGLGDYAPGLHGGLEPGLDSSLDPRLDPALLQGVEIDPDGLAVSESVHIVEGMYSELHSVAAEVGIPVTATHFDLQEELLWMGNHRGHASSFFGPTMGRYSSFQVHMTDDIRHIQSMDTGVLFLTKNNLRCLTRGGFIMFDYPMEEGADMHSLILTENNTLLMGGLQNYVAELDLTTVQETQKFTVEVPGVAIMRQTSRFFFCGHTSGKVSLRDLRTFKMEHEFDAFSGSLSDFDIHGNLLAACGFSSRGLNGLSCDRFLMVYDLRMMRAVTPLQVHVDPLFLRFIPTYTSRLAIISQTGQCQFCEPTGLASLADIFHVNTVGQLLMSFDVSSSKQALSFGDSGGCVHLWSSAPEVSYNSYSRDTELALPCLVDSLPQMDWNHELMPLSLLPMPLTSTEPLLSDWPTALIAPSPRRAPPVDPEILRTMKTVGFIGYAPNPRTRPRNQVPYKIKDVELDYDSYNQVPESPIGRDEEPHLYMVPKKYRKVTIKYSKLGLEDFDFKHYNRTLFAGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILAQLHQETLEQEGPQAYRGASSSSVLSSSGESVISRLFGCEVENSSLCCCGKETVRSSLTLLFTMHYPEQSSLEKKIEEYEFVDILRKSICLEQSTQAWCDNCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKAQTEYVFNKALKKEAAEPPKPKEPLPTEWCLEEELCNVEGLTFDTQAEDMRHVWIPLSLRMCISKAQGLEVSSLTEGEELTESEEAEGVSIYDLFVTVSHVQDTRTGGNMVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKAESVQFDVNWKVPAVLYYIKRNYHTKFDLRIKNPIEASVLLTEASLARKQRKSHATFIPLMVSEMPQVGDLVGLDAEFVTLNQEEAELRSDGTKSTIKPSQMSVARITCVRGQGQNEGVPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDTGVRFVGHGLQKDFRVINLMVPKDQVIDTVYLFHMPRKRMISLRFLAWYFLDLNIQGETHDSIEDARTALQLYRKYLELSHNGGQEEFRKVLKALYEKGRKLDWKVPDIDSADGQGSPKSTAVFPSVMGL

Target ORF information:

RefSeq Version	XM_005162345.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162345.3

ORF Insert Sequence:

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGTAGCC CAAAAAGCAC AGCCGTATTT 
CCCTCAGTGA TGGGTTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pan2 ( XM_001919965.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001920000.4 Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X2, mRNA.

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CloneID	ODa27932
Clone ID Related Accession (Same CDS sequence)	XM_001919965.6 , XM_001919965.5
Accession Version	XM_001919965.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919965.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGCACAG CCGTATTTCC CTCAGTGATG 
GGTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001920000.4
CDS	90..3698
Translation	MMNFEGLDPGLGDYAPGLHGGLEPGLDSSLDPRLDPALLQGVEIDPDGLAVSESVHIVEGMYSELHSVAAEVGIPVTATHFDLQEELLWMGNHRGHASSFFGPTMGRYSSFQVHMTDDIRHIQSMDTGVLFLTKNNLRCLTRGGFIMFDYPMEEGADMHSLILTENNTLLMGGLQNYVAELDLTTVQETQKFTVEVPGVAIMRQTSRFFFCGHTSGKVSLRDLRTFKMEHEFDAFSGSLSDFDIHGNLLAACGFSSRGLNGLSCDRFLMVYDLRMMRAVTPLQVHVDPLFLRFIPTYTSRLAIISQTGQCQFCEPTGLASLADIFHVNTVGQLLMSFDVSSSKQALSFGDSGGCVHLWSSAPEVSYNSYSRDTELALPCLVDSLPQMDWNHELMPLSLLPMPLTSTEPLLSDWPTALIAPSPRRAPPVDPEILRTMKTVGFIGYAPNPRTRPRNQVPYKIKDVELDYDSYNQVPESPIGRDEEPHLYMVPKKYRKVTIKYSKLGLEDFDFKHYNRTLFAGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILAQLHQETLEQEGPQAYRGASSSSVLSSSGESVISRLFGCEVENSSLCCCGKETVRSSLTLLFTMHYPEQSSLEKKIEEYEFVDILRKSICLEQSTQAWCDNCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKAQTEYVFNKALKKEAAEPPKPKEPLPTEWCLEEELCNVEGLTFDTQAEDMRHVWIPLSLRMCISKAQGLEVSSLTEGEELTESEEAEGVSIYDLFVTVSHVQDTRTGGNMVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKAESVQFDVNWKVPAVLYYIKRNYHTKFDLRIKNPIEASVLLTEASLARKQRKSHATFIPLMVSEMPQVGDLVGLDAEFVTLNQEEAELRSDGTKSTIKPSQMSVARITCVRGQGQNEGVPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDTGVRFVGHGLQKDFRVINLMVPKDQVIDTVYLFHMPRKRMISLRFLAWYFLDLNIQGETHDSIEDARTALQLYRKYLELSHNGGQEEFRKVLKALYEKGRKLDWKVPDIDSADGQGTAVFPSVMGL

Target ORF information:

RefSeq Version	XM_001919965.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919965.6

ORF Insert Sequence:

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGCACAG CCGTATTTCC CTCAGTGATG 
GGTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pan2 ( XM_001919965.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001920000.4 Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X2, mRNA.

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CloneID	ODa27932
Clone ID Related Accession (Same CDS sequence)	XM_001919965.6 , XM_001919965.5
Accession Version	XM_001919965.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919965.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGCACAG CCGTATTTCC CTCAGTGATG 
GGTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001920000.4
CDS	92..3700
Translation	MMNFEGLDPGLGDYAPGLHGGLEPGLDSSLDPRLDPALLQGVEIDPDGLAVSESVHIVEGMYSELHSVAAEVGIPVTATHFDLQEELLWMGNHRGHASSFFGPTMGRYSSFQVHMTDDIRHIQSMDTGVLFLTKNNLRCLTRGGFIMFDYPMEEGADMHSLILTENNTLLMGGLQNYVAELDLTTVQETQKFTVEVPGVAIMRQTSRFFFCGHTSGKVSLRDLRTFKMEHEFDAFSGSLSDFDIHGNLLAACGFSSRGLNGLSCDRFLMVYDLRMMRAVTPLQVHVDPLFLRFIPTYTSRLAIISQTGQCQFCEPTGLASLADIFHVNTVGQLLMSFDVSSSKQALSFGDSGGCVHLWSSAPEVSYNSYSRDTELALPCLVDSLPQMDWNHELMPLSLLPMPLTSTEPLLSDWPTALIAPSPRRAPPVDPEILRTMKTVGFIGYAPNPRTRPRNQVPYKIKDVELDYDSYNQVPESPIGRDEEPHLYMVPKKYRKVTIKYSKLGLEDFDFKHYNRTLFAGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILAQLHQETLEQEGPQAYRGASSSSVLSSSGESVISRLFGCEVENSSLCCCGKETVRSSLTLLFTMHYPEQSSLEKKIEEYEFVDILRKSICLEQSTQAWCDNCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKAQTEYVFNKALKKEAAEPPKPKEPLPTEWCLEEELCNVEGLTFDTQAEDMRHVWIPLSLRMCISKAQGLEVSSLTEGEELTESEEAEGVSIYDLFVTVSHVQDTRTGGNMVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKAESVQFDVNWKVPAVLYYIKRNYHTKFDLRIKNPIEASVLLTEASLARKQRKSHATFIPLMVSEMPQVGDLVGLDAEFVTLNQEEAELRSDGTKSTIKPSQMSVARITCVRGQGQNEGVPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDTGVRFVGHGLQKDFRVINLMVPKDQVIDTVYLFHMPRKRMISLRFLAWYFLDLNIQGETHDSIEDARTALQLYRKYLELSHNGGQEEFRKVLKALYEKGRKLDWKVPDIDSADGQGTAVFPSVMGL

Target ORF information:

RefSeq Version	XM_001919965.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (pan2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919965.5

ORF Insert Sequence:

ATGATGAACT TCGAGGGTCT GGACCCGGGT TTGGGCGATT ATGCGCCTGG CCTGCATGGT 
GGCCTGGAGC CCGGTCTGGA CTCTTCCCTG GACCCGCGGC TCGACCCGGC GCTACTGCAG 
GGTGTGGAGA TCGACCCCGA TGGGCTGGCT GTGTCAGAGT CTGTGCACAT AGTGGAAGGC 
ATGTACTCGG AGTTGCACAG CGTGGCAGCT GAAGTCGGGA TCCCTGTGAC AGCCACACAC 
TTCGATCTGC AGGAGGAGTT GCTCTGGATG GGGAACCACC GGGGACACGC GAGTTCCTTC 
TTTGGGCCGA CAATGGGCCG CTACTCGTCT TTCCAGGTGC ACATGACTGA CGACATTCGC 
CATATCCAGA GTATGGACAC GGGGGTGCTG TTCCTGACCA AAAACAACCT CAGATGCCTC 
ACACGAGGAG GTTTTATCAT GTTTGATTAT CCAATGGAGG AGGGTGCTGA CATGCACAGC 
TTGATTCTTA CTGAAAATAA CACCTTGTTA ATGGGAGGCC TGCAGAACTA TGTGGCTGAA 
CTGGACCTCA CCACAGTGCA GGAAACTCAA AAATTTACTG TTGAAGTGCC TGGTGTCGCC 
ATCATGAGAC AAACTAGTCG TTTCTTCTTT TGTGGTCACA CATCTGGCAA GGTGTCTCTT 
CGTGATCTGC GCACGTTTAA AATGGAGCAC GAGTTTGATG CATTCTCCGG CAGCCTGTCT 
GATTTCGACA TTCACGGTAA CCTGTTAGCG GCATGTGGTT TCTCAAGTCG CGGTCTGAAC 
GGGCTGTCCT GTGATCGGTT CCTCATGGTG TACGATCTGC GCATGATGAG GGCCGTCACC 
CCATTGCAGG TCCACGTGGA CCCCCTATTC CTCCGCTTTA TCCCCACATA CACCTCCCGC 
CTGGCCATCA TTTCACAAAC AGGTCAGTGT CAGTTCTGTG AGCCCACCGG CCTGGCCAGC 
CTGGCTGATA TCTTCCACGT CAACACAGTG GGCCAGCTGC TGATGAGCTT TGACGTGTCC 
TCCAGCAAGC AGGCTCTGTC ATTCGGGGAC TCGGGCGGCT GTGTGCATCT GTGGTCCAGC 
GCCCCTGAGG TGTCCTATAA CAGCTACTCT AGAGACACAG AGCTGGCGCT GCCCTGCCTG 
GTGGACTCAC TGCCGCAGAT GGACTGGAAC CACGAGCTGA TGCCTCTGTC TCTTCTGCCA 
ATGCCTCTCA CCAGCACTGA GCCTCTGCTG TCTGACTGGC CCACCGCCCT CATCGCACCC 
AGCCCCAGAC GAGCGCCTCC AGTAGACCCA GAGATTCTGA GGACAATGAA AACTGTGGGA 
TTTATTGGAT ACGCACCAAA TCCTAGAACC CGTCCCAGAA ACCAGGTTCC ATATAAGATA 
AAAGATGTCG AGCTTGACTA TGACAGCTAT AATCAGGTGC CGGAGTCTCC CATTGGTCGA 
GACGAGGAGC CACATCTTTA CATGGTGCCA AAGAAATACA GGAAGGTGAC CATTAAATAT 
TCCAAACTGG GACTGGAAGA CTTTGATTTT AAGCACTATA ATCGGACTCT GTTTGCTGGT 
CTGGAGCCTC ACATACCCAA CGCTTACTGT AACTGTATGA TCCAGGTTTT GTACTTCCTG 
GAGCCCATCA GGTGTCTGGT ACAGAATCAC CTGTGTCAGA AAGAGTTCTG CCTGGCCTGT 
GAGCTGGGCT TCCTCTTCCA CATGCTGGAC CTGAGCAGAG GAGACCCCTG TCAGGCCAGT 
AATTTTCTCC GGGCTTTCCG GACAATTCCC GAGGCATCTG CTCTGGGTTT AATCCTGGCT 
GACTCCGATG AGCAGACTGG GAAAGCCCGT CTGGGCCGTC TAATCCAGAG CTGGAACCGT 
TTTATTCTTG CACAGCTGCA TCAGGAAACG CTCGAGCAGG AAGGGCCGCA GGCGTACAGA 
GGAGCCAGCA GCAGCAGTGT GCTGAGCTCC TCAGGGGAGT CTGTGATTAG CCGGTTGTTT 
GGCTGTGAAG TGGAGAACAG CAGTCTGTGT TGTTGTGGAA AGGAGACGGT TCGCTCTTCA 
TTGACGCTGC TGTTTACCAT GCATTACCCC GAGCAGAGCT CCCTGGAGAA GAAGATAGAG 
GAATATGAAT TTGTAGATAT TCTGAGGAAG AGTATCTGTC TGGAACAGAG CACACAGGCC 
TGGTGTGACA ACTGTGAGAA GTACCAGCCA ACAGTGCAGA CCAGGAACAT TCGCTGTCTG 
CCTGATGTTC TGGTGATAAA CTGTGAGGTG AACAGTGCAA AAGAGGCAGA ATTCTGGAAG 
GCACAGACTG AGTATGTGTT CAACAAAGCT TTGAAGAAAG AAGCAGCCGA ACCTCCCAAA 
CCTAAAGAGC CTTTACCCAC AGAATGGTGT TTGGAAGAAG AGCTGTGCAA TGTGGAGGGC 
TTGACCTTTG ACACTCAAGC AGAAGACATG AGGCATGTGT GGATTCCTCT CAGTCTGAGG 
ATGTGCATCA GTAAAGCTCA AGGCCTGGAG GTCAGCAGCC TAACTGAGGG AGAGGAGCTG 
ACTGAAAGTG AAGAGGCAGA AGGAGTGTCC ATCTACGATC TGTTTGTCAC TGTGTCGCAT 
GTCCAGGACA CTCGGACTGG AGGAAACATG GTGGCGCACA TCAAAGTGGG CGAGACTTAT 
CATCAGCGCA AAGAGGGAGT CACACACCAG CAGTGGTATC TGTTCAATGA TTTTCTGATT 
GAGCCCATTG ATAAGGCTGA ATCGGTTCAG TTTGATGTGA ACTGGAAGGT TCCTGCTGTA 
CTTTATTACA TCAAGAGGAA CTACCACACC AAGTTTGATT TGCGCATTAA GAACCCTATC 
GAGGCAAGCG TTCTGCTGAC GGAGGCTTCT TTAGCACGAA AACAGAGGAA GAGTCACGCC 
ACCTTCATAC CCCTCATGGT GAGCGAAATG CCACAGGTTG GAGACCTTGT CGGATTGGAT 
GCAGAATTTG TCACTCTCAA CCAGGAAGAG GCAGAACTGC GCAGCGATGG AACTAAGTCC 
ACTATTAAAC CCAGCCAGAT GTCTGTTGCT CGTATCACTT GTGTGAGAGG CCAGGGACAA 
AATGAAGGAG TGCCGTTCAT TGATGACTAC ATATCTACAC AGGAACAGGT GGTAGACTAT 
CTGACCCAGT ATTCTGGTAT CAAACCTGGA GATTTGGATG CAAAGATTTC CTCGAAACAC 
CTGACTACTC TGAAATCCAC ATATCTTAAA TTGCGGTTTC TTATTGACAC TGGGGTTCGC 
TTTGTCGGGC ACGGCTTACA GAAGGATTTC CGGGTCATAA ACCTGATGGT CCCAAAAGAC 
CAGGTGATCG ACACTGTCTA CCTCTTTCAC ATGCCTAGGA AACGAATGAT CTCTCTGCGG 
TTCCTTGCAT GGTATTTTCT TGATTTGAAC ATCCAGGGTG AAACTCATGA CAGTATTGAA 
GATGCGCGGA CTGCTTTGCA GTTGTACAGG AAGTACCTAG AGCTGAGCCA TAATGGAGGA 
CAGGAGGAGT TCCGGAAGGT TCTCAAGGCT CTCTATGAGA AAGGACGCAA GCTAGACTGG 
AAAGTTCCAG ACATCGATTC TGCAGATGGT CAAGGCACAG CCGTATTTCC CTCAGTGATG 
GGTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pan2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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