gnl2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gnl2
Entrez Gene ID	793727
Full Name	guanine nucleotide binding protein-like 2 (nucleolar)
Synonyms	wu:fc15c12,zgc:65800,zgc:77319
General protein information	Preferred Names guanine nucleotide binding protein-like 2 (nucleolar) Names nucleolar GTP-binding protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213224.1	NP_998389.1	nucleolar GTP-binding protein 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gnl2	NP_001020907.1
Gallus gallus (chicken)	GNL2	XP_417761.3
Drosophila melanogaster (fruit fly)	ns2	NP_611232.1
Caenorhabditis elegans (roundworm)	ngp-1	NP_001021621.1
Saccharomyces cerevisiae (baker's yeast)	NOG2	NP_014451.1
Homo sapiens (human)	GNL2	NP_037417.1
Danio rerio (zebrafish)	gnl2	NP_998389.1
Schizosaccharomyces pombe (fission yeast)	SPAC6F6.03c	NP_593896.1
Neurospora crassa	NCU02546	XP_965677.1
Canis lupus familiaris (dog)	GNL2	XP_532556.2
Mus musculus (house mouse)	Gnl2	NP_663527.2
Danio rerio (zebrafish)	LOC100536859	XP_003200256.1
Eremothecium gossypii	AGOS_ABR120C	NP_983067.1
Magnaporthe oryzae (rice blast fungus)	MGG_01561	XP_003714536.1
Oryza sativa (rice)	Os03g0352400	NP_001050123.1
Pan troglodytes (chimpanzee)	GNL2	NP_001233327.1
	GNL2	XP_001111847.2
Bos taurus (cattle)	GNL2	NP_001039432.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011309	XP_309340.4
Kluyveromyces lactis	KLLA0C18843g	XP_453040.1
Arabidopsis thaliana (thale cress)	AT1G52980	NP_175706.1
Xenopus tropicalis (tropical clawed frog)	gnl2	NP_001001243.1

Gene Ontology
Bibliography

Related articles in PubMed

Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation.
Gallagher TL, Tietz KT, Morrow ZT, McCammon JM, Goldrich ML, Derr NL, Amacher SL
Developmental biology429(1)225-239(2017 09)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis.
Essers PB, Pereboom TC, Goos YJ, Paridaen JT, Macinnes AW
Developmental biology385(2)304-15(2014 Jan)

The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish.
Paridaen JT, Janson E, Utami KH, Pereboom TC, Essers PB, van Rooijen C, Zivkovic D, MacInnes AW
Developmental biology355(2)286-301(2011 Jul)

Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach.
Encinas P, Rodriguez-Milla MA, Novoa B, Estepa A, Figueras A, Coll J
BMC genomics11518(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish.
Title: The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish.

The following gnl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gnl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00305	NM_213224.1 Latest version!	Danio rerio guanine nucleotide binding protein-like 2 (nucleolar) (gnl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gnl2 ( NM_213224.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998389.1 Danio rerio guanine nucleotide binding protein-like 2 (nucleolar) (gnl2), mRNA.

Price & Availability↑

CloneID	ODa00305
Clone ID Related Accession (Same CDS sequence)	NM_213224.1
Accession Version	NM_213224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Danio rerio(zebrafish)
Product	nucleolar GTP-binding protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065960.1. On Mar 1, 2008 this sequence version replaced XM_001332987.1. ##Evidence-Data-START## Transcript exon combination :: BC065960.1, BC056293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTTAAGG CAAAATTCAA GGGGAAAAGC TCCATAAACA CCTCAAACTC CAGTAGTAAT 
CCCGACCGAG TCAAGGGAGC TGGAGGAAAC AACATGAGGG ACCGAGCAAC TATAAAGCGC 
CTGAATATGT ACAGACAAAA GCAGAGATGT AACAGTCGAG GTAAAGTCAT CAAACCTTTG 
CAGTACCAGA ACACAGTCGC TCCTGGAACT GTGGCCAGAG TTGAGCCCAA CATCAAGTGG 
TTTGCCAACA CAAAAGTCAT CAAGCAGTCA TCCTTGCAGA AGTTTCAGGA AGAAATGAAC 
GCGGTGAAGA AAGATCCATA CCGAGTGGTG ATGAGACAGA GCAAACTACC AATGTCCTTG 
CTGCATGACC GGATAAAAGC ACACAACTCC AAGGTGCATA TTCTAGACAC TGAGACGTTT 
GAGACAACAT TTGGACCTAA GGCGCAGAGA AAGAGGCCAA ACTTGTCTGT AGGAGAGCTG 
AAAGAGTTTG CTGAACAGGC TGAGGTCTCT GCACAGAGCT ACAGTGCAGA GAAAGACCGC 
GATTTGGTGT CTGAGGACAG CGGAGTCAAG GAAGAGGCTC GTGAGGAGAT CTTCAAGAAG 
GGGCAGTCAA AACGGATCTG GGGTGAGCTT TATAAGGTGA TCGACTCTTC GGACGTAATT 
ATCCAGGTGC TGGATGCTCG GGACCCAATG GGCACACGCT CACAAAGCAT TGAGACTTAC 
TTGAAGAAAG AGAAGCCTTG GAAACACCTC ATATTTGTGC TCAATAAGTG TGACCTCATT 
CCCACATGGG TTACGAAACA CTGGGTTGCA GTTCTTTCCC AGGAATACCC TACTTTGGCC 
TTTCACGCAA GCCTTACCAA CTCCTTCGGC AAGGGCTCAC TTATCCAGCT CCTGCGGCAG 
TTTGGCAAGC TCCATTCCGA CAAAAAACAG ATCAGTGTTG GTTTCATTGG TTACCCCAAC 
GTTGGAAAGA GCTCTATTAT CAATACCCTG CGCTCCAAAA AAGTCTGTAA TGTGGCACCC 
CTTGCTGGTG AAACTAAGGT TTGGCAGTAC ATCACACTAA TGAGACGGAT TTTCCTCATC 
GACTGTCCTG GCGTTGTTTA CCCGTCTGAC GACAGCGAGA CTGACATTGT GCTGAAGGGA 
GTGGTCCAAG TAGAGAAGAT CCGAAATCCA GAGGATCACA TTGGAGCAGT GCTGGAAAGA 
GCCAAAGCAG AGTACATCCA GAAGACATAC CGCATTCCCT CCTGGAGCTC TGCCGAGGAT 
TTCCTGGAAA AACTCGCCTT TCGCACCGGA AAACTGCTTA AGGGTGGAGA ACCAGATCTG 
CCTACTGTGT CCAAAATGGT ACTGAATGAT TGGCAGAGGG GACGCATCCC GTTTTTTGTC 
AAGCCCCCTG GCGTTGAGAC AGACGAAGAG AACAAAGCCC AGGCGATGCT GGAAATGCCG 
GAGATGGAAA GCGAGATGGA TGAGTTGGAA AACAGACTTG AAGCGCAGGA TGTGGAAGAG 
GCCTCATCAA GCCAGCATGA AGAACAAAAG CTGCTGAAAA AGAAGAAGGA AGAGGTGCAG 
AAACTCTTCA ATGATGTCAA GCAGAACTTT GGCAAAATTA ACGTCGCCCC GGAGTTCAGC 
GAGGAAGACC TTGTGCCTGT GGAGATGCCG GACATGATGG ATGCTTCCGG CTCAGAAGAT 
GAGGAAAATG AGGAGGAGGA GGAGGAGGAG GATGATGAGG AGAAAGCGGA AGAGGAGACT 
GGAGAACAGC AGGAAGAAGA ATCCTCTGTT TCAGACTCAA CACCCAAAGC AGGGAAAAAG 
AGCACACAGG AAGTAAACAA AGAGATGGAT GAGAAGATTG CTAAATTGAA ACATTTTCTT 
GATCGAGCCA AGTCAAAGCA CTTCTCCGCC ATACGCATTC CTAAAGGCCT TTCTGAAAGG 
GTGTTTACTG AAACAGTAGC AAAGCAGAAC GCTGCAAAGA ATCAACAACA GAAAGCTGTA 
AAACAAGGCA AACGGAGGAG AGCTGTAGAA GATGAGCCTG CACAGCCTGC AAAACTCACA 
TCTAAAGAGA AAAGGAGAAT TGAGCGTGCC CAGAAAGTGA AGAAAGTGGG AGTCCGCTAT 
TATGAGACGC ACAACGTCAA GAACAAGAAT AAGAACAGAA AAATTCCCTC AGATGGCAAG 
AAAGCCAATC GAGCCAAGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998389.1
CDS	74..2257
Translation	MVKAKFKGKSSINTSNSSSNPDRVKGAGGNNMRDRATIKRLNMYRQKQRCNSRGKVIKPLQYQNTVAPGTVARVEPNIKWFANTKVIKQSSLQKFQEEMNAVKKDPYRVVMRQSKLPMSLLHDRIKAHNSKVHILDTETFETTFGPKAQRKRPNLSVGELKEFAEQAEVSAQSYSAEKDRDLVSEDSGVKEEAREEIFKKGQSKRIWGELYKVIDSSDVIIQVLDARDPMGTRSQSIETYLKKEKPWKHLIFVLNKCDLIPTWVTKHWVAVLSQEYPTLAFHASLTNSFGKGSLIQLLRQFGKLHSDKKQISVGFIGYPNVGKSSIINTLRSKKVCNVAPLAGETKVWQYITLMRRIFLIDCPGVVYPSDDSETDIVLKGVVQVEKIRNPEDHIGAVLERAKAEYIQKTYRIPSWSSAEDFLEKLAFRTGKLLKGGEPDLPTVSKMVLNDWQRGRIPFFVKPPGVETDEENKAQAMLEMPEMESEMDELENRLEAQDVEEASSSQHEEQKLLKKKKEEVQKLFNDVKQNFGKINVAPEFSEEDLVPVEMPDMMDASGSEDEENEEEEEEEDDEEKAEEETGEQQEEESSVSDSTPKAGKKSTQEVNKEMDEKIAKLKHFLDRAKSKHFSAIRIPKGLSERVFTETVAKQNAAKNQQQKAVKQGKRRRAVEDEPAQPAKLTSKEKRRIERAQKVKKVGVRYYETHNVKNKNKNRKIPSDGKKANRAKR

Target ORF information:

RefSeq Version	NM_213224.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanine nucleotide binding protein-like 2 (nucleolar) (gnl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213224.1

ORF Insert Sequence:

ATGGTTAAGG CAAAATTCAA GGGGAAAAGC TCCATAAACA CCTCAAACTC CAGTAGTAAT 
CCCGACCGAG TCAAGGGAGC TGGAGGAAAC AACATGAGGG ACCGAGCAAC TATAAAGCGC 
CTGAATATGT ACAGACAAAA GCAGAGATGT AACAGTCGAG GTAAAGTCAT CAAACCTTTG 
CAGTACCAGA ACACAGTCGC TCCTGGAACT GTGGCCAGAG TTGAGCCCAA CATCAAGTGG 
TTTGCCAACA CAAAAGTCAT CAAGCAGTCA TCCTTGCAGA AGTTTCAGGA AGAAATGAAC 
GCGGTGAAGA AAGATCCATA CCGAGTGGTG ATGAGACAGA GCAAACTACC AATGTCCTTG 
CTGCATGACC GGATAAAAGC ACACAACTCC AAGGTGCATA TTCTAGACAC TGAGACGTTT 
GAGACAACAT TTGGACCTAA GGCGCAGAGA AAGAGGCCAA ACTTGTCTGT AGGAGAGCTG 
AAAGAGTTTG CTGAACAGGC TGAGGTCTCT GCACAGAGCT ACAGTGCAGA GAAAGACCGC 
GATTTGGTGT CTGAGGACAG CGGAGTCAAG GAAGAGGCTC GTGAGGAGAT CTTCAAGAAG 
GGGCAGTCAA AACGGATCTG GGGTGAGCTT TATAAGGTGA TCGACTCTTC GGACGTAATT 
ATCCAGGTGC TGGATGCTCG GGACCCAATG GGCACACGCT CACAAAGCAT TGAGACTTAC 
TTGAAGAAAG AGAAGCCTTG GAAACACCTC ATATTTGTGC TCAATAAGTG TGACCTCATT 
CCCACATGGG TTACGAAACA CTGGGTTGCA GTTCTTTCCC AGGAATACCC TACTTTGGCC 
TTTCACGCAA GCCTTACCAA CTCCTTCGGC AAGGGCTCAC TTATCCAGCT CCTGCGGCAG 
TTTGGCAAGC TCCATTCCGA CAAAAAACAG ATCAGTGTTG GTTTCATTGG TTACCCCAAC 
GTTGGAAAGA GCTCTATTAT CAATACCCTG CGCTCCAAAA AAGTCTGTAA TGTGGCACCC 
CTTGCTGGTG AAACTAAGGT TTGGCAGTAC ATCACACTAA TGAGACGGAT TTTCCTCATC 
GACTGTCCTG GCGTTGTTTA CCCGTCTGAC GACAGCGAGA CTGACATTGT GCTGAAGGGA 
GTGGTCCAAG TAGAGAAGAT CCGAAATCCA GAGGATCACA TTGGAGCAGT GCTGGAAAGA 
GCCAAAGCAG AGTACATCCA GAAGACATAC CGCATTCCCT CCTGGAGCTC TGCCGAGGAT 
TTCCTGGAAA AACTCGCCTT TCGCACCGGA AAACTGCTTA AGGGTGGAGA ACCAGATCTG 
CCTACTGTGT CCAAAATGGT ACTGAATGAT TGGCAGAGGG GACGCATCCC GTTTTTTGTC 
AAGCCCCCTG GCGTTGAGAC AGACGAAGAG AACAAAGCCC AGGCGATGCT GGAAATGCCG 
GAGATGGAAA GCGAGATGGA TGAGTTGGAA AACAGACTTG AAGCGCAGGA TGTGGAAGAG 
GCCTCATCAA GCCAGCATGA AGAACAAAAG CTGCTGAAAA AGAAGAAGGA AGAGGTGCAG 
AAACTCTTCA ATGATGTCAA GCAGAACTTT GGCAAAATTA ACGTCGCCCC GGAGTTCAGC 
GAGGAAGACC TTGTGCCTGT GGAGATGCCG GACATGATGG ATGCTTCCGG CTCAGAAGAT 
GAGGAAAATG AGGAGGAGGA GGAGGAGGAG GATGATGAGG AGAAAGCGGA AGAGGAGACT 
GGAGAACAGC AGGAAGAAGA ATCCTCTGTT TCAGACTCAA CACCCAAAGC AGGGAAAAAG 
AGCACACAGG AAGTAAACAA AGAGATGGAT GAGAAGATTG CTAAATTGAA ACATTTTCTT 
GATCGAGCCA AGTCAAAGCA CTTCTCCGCC ATACGCATTC CTAAAGGCCT TTCTGAAAGG 
GTGTTTACTG AAACAGTAGC AAAGCAGAAC GCTGCAAAGA ATCAACAACA GAAAGCTGTA 
AAACAAGGCA AACGGAGGAG AGCTGTAGAA GATGAGCCTG CACAGCCTGC AAAACTCACA 
TCTAAAGAGA AAAGGAGAAT TGAGCGTGCC CAGAAAGTGA AGAAAGTGGG AGTCCGCTAT 
TATGAGACGC ACAACGTCAA GAACAAGAAT AAGAACAGAA AAATTCCCTC AGATGGCAAG 
AAAGCCAATC GAGCCAAGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation.
Developmental biology429(1)225-239(2017 09)
Gallagher TL,Tietz KT,Morrow ZT,McCammon JM,Goldrich ML,Derr NL,Amacher SL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis.
Developmental biology385(2)304-15(2014 Jan)
Essers PB,Pereboom TC,Goos YJ,Paridaen JT,Macinnes AW

The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish.
Developmental biology355(2)286-301(2011 Jul)
Paridaen JT,Janson E,Utami KH,Pereboom TC,Essers PB,van Rooijen C,Zivkovic D,MacInnes AW

Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach.
BMC genomics11518(2010 Sep)
Encinas P,Rodriguez-Milla MA,Novoa B,Estepa A,Figueras A,Coll J