ddx21 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx21
Entrez Gene ID	799650
Full Name	DEAD (Asp-Glu-Ala-Asp) box helicase 21
Synonyms	cb960,wu:fi30c07,wu:fi34d08
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box helicase 21 Names nucleolar RNA helicase 2 DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358815.1	XP_017214304.1	nucleolar RNA helicase 2 isoform X1
NM_001127335.2	NP_001120807.2	nucleolar RNA helicase 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ddx21	NP_001039224.1
Bos taurus (cattle)	DDX21	NP_001076996.1
Pan troglodytes (chimpanzee)	DDX21	XP_507825.2
	DDX21	XP_001110939.1
Gallus gallus (chicken)	DDX50	XP_001232052.1
Danio rerio (zebrafish)	ddx21	NP_001120807.1
Homo sapiens (human)	DDX21	NP_004719.2
Canis lupus familiaris (dog)	DDX21	XP_851746.2
Mus musculus (house mouse)	Ddx21	NP_062426.2
Rattus norvegicus (Norway rat)	Ddx21	NP_001032278.1
Arabidopsis thaliana (thale cress)	PRH75	NP_201025.1
Oryza sativa (rice)	Os09g0520700	NP_001063702.1

Gene Ontology
Bibliography

Related articles in PubMed

Investigation of the effects of estrogen on skeletal gene expression during zebrafish larval head development.
Pashay Ahi E, Walker BS, Lassiter CS, J?nsson ZO
PeerJ4e1878(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Feng H, Zhang YB, Zhang QM, Li Z, Zhang QY, Gui JF
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)

TET2 plays an essential role in erythropoiesis by regulating lineage-specific genes via DNA oxidative demethylation in a zebrafish model.
Ge L, Zhang RP, Wan F, Guo DY, Wang P, Xiang LX, Shao JZ
Molecular and cellular biology34(6)989-1002(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddx21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa60484	NM_001127335.2 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa54749	XM_017358815.1 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx21 ( NM_001127335.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001120807.2 Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), mRNA.

Price & Availability↑

CloneID	ODa60484
Clone ID Related Accession (Same CDS sequence)	NM_001127335.2
Accession Version	NM_001127335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nucleolar RNA helicase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FQ378000.6, EH525400.1, DT075062.1, DT065140.1, EH437022.1, EB906270.1 and EH463012.1. On Nov 5, 2015 this sequence version replaced NM_001127335.1. ##Evidence-Data-START## Transcript exon combination :: BC160629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476728, SAMEA4476741 [ECO:0000348] ##Evidence-Data-END##

ATGCGCACTT CCGCTTTGCG CACGTCCGCT TCCGTCCTTT CCTTTCCTCA CGTGTGTTGT 
GAGAGCTCCA TGTCCAGCCG CTCCTCAACA TCCAGCATGC CGTCAAAGAG TCTCCCAGAA 
TCGGAAGCCG AGAGAGACCA CCTGATGGAG GACATTCACA ACGGCCTGCA GCTGCTCAAG 
AAGAAGAAGA AGAAGCTGAA GGAGCAGCAG CAGACGGCAG AAACAGACGA GTGTGAGTCT 
CCTGCTCCTA AAAAGAGGAA GATGAAGATT TCCAGCGACA CCACAGACCT GAACGGAAAC 
ACAGACACAC ACACACTCAA GGTCAAGAAG AAGAAGATCA AGCTGGAGGA GGAGGAGGAG 
GAGACGACGA GCACTCTGCT GGAGAAGAAG AAGAAGAAGA AGATCAAGCT GGAGAAGGAG 
GAGATGATGG AGGAGACGAA CACTCTGCTG GAGAAGAAGA AGAAGATCAA GATGGAGAAA 
GAGGAGGAGA AGATGATGAA CGGAGACTCA CACACACACT CCACAGAGCC TGCAGCGTCC 
AGCACAGACT CCGACAGCGA ACAGGAAAAA GAGACTCCAG AGCAGAGAGA AGGAGCTTTC 
TCCAACTTCA GGATCTCACC GAACACCATC AAACTGCTGC AGGCTCGTGG CGTCTCGTAC 
CTGTTTGACA TCCAGGTGAA GACGTTTAAT GCAGTGTATG ATGGGAAGGA TCTGATTGGT 
CAGGCCCGGA CCGGCACCGG GAAGACCTTC TCCTTCGCTG TTCCTCTGGT GGAGAAGCTG 
CAGTCTGGAG ACCAGGAGCG GCGGAGAGGA CGACCACCAA AGGTTCTGGT TCTGGCTCCG 
ACTCGTGAGC TGGCCATACA GGTGACCAAA GACTTCAAGG ACATCACCAG GAAACTCTCC 
GTCACCTGCT TCTATGGAGG AAGCTCATAC AACCCTCAGA TCGATGCGAT CCGCAGTGGC 
ATTGACGTCC TGGTGGGGAC GCCGGGCCGA ATCCGAGACC ATCTGCAGAA CAATAAGCTG 
GATCTGTCTC AGCTGCAGCA TGTGGTGCTG GACGAGGTGG ACCAGATGCT GGACATGGGC 
TTCGCTGAGC AGGTGGAGGA GATCCTGTCT GCCTCCTACA AGAAAGATGC GGAGCAGAAG 
CCGCAGACGC TGCTGTTCTC CGCCACGTGT CCCTCATGGG TTTATGACGT GGCCAAGAAA 
TACATGCGCT CACAGTTCAT CCACGTGGAC CTCATCGGGA AGAAGACACA GAAAGCTGCA 
ACCACTGTCG AGCACCTGGC CATCGCATGT CACTGGTCTC AGAGGGCGTC GGTCATCGGT 
GATGTCATCC AGGTGTACAG CGGCAGCCAC GGCCGCACCA TCGTCTTCTG TGAGACCAAG 
AAGGAGGCGA CAGAGCTGTC CCTGAACACC TCCATCAAAC AGAGTGCTCA GTCTCTGCAT 
GGAGATATCC CGCAGAAGCA GAGGGAGGTG ACGCTGAAGG GTTTCAGGAA CGGCTCTTTC 
GAGGTGTTGG TGGCCACTAA TGTAGCTGCG CGCGGGCTGG ACATCCCAGA GGTGGACCTG 
GTCATCCAGA GCTCACCACC AAACGATGTG GAGTCGTATA TCCACCGGTC GGGCCGGACG 
GGCAGAGCTG GAAGAACCGG CATCTGCATC TGCTTCTACC AGCGCAAAGA GGAGAGCCAG 
CTGAAGTTTG TGGAGCAGAA AGCGGGGATC ACCTTTAAGC GAGTGGGCGT TCCCACAGCC 
TATGACATCA TCCAGTCCTC CAGTAAAGAC GCCGTCAGGT TTCTGGACTC GGTGCCGGCA 
GTAGCGGTGG GATATTTCCG TGAGGCGGCG CAGGAGCTGA TCCAGCAGCG GGGGGCAGTG 
GAGGCTCTGG CAGCAGCACT CGCTCACATA TCAGGAGCCA CCAGCCTGGA GCAGAGATCC 
CTGATCAACT CTGAGCCTGG TTTCTCCAGC ATGATCCTCA GCTGCTCGCA GGAGATGCAC 
AGCATAAGTT TCGCCTGGAG AGGACTGAAG GAGCAGCTGG GAGAGGAGAT AGACCAGCAC 
ATCTCACACA TGGTCTTCAT CAAGGGGAAG AAGGGTGTGT GTTTCGACGT CCCAGTCAGC 
AAACTGAAGG AGGTCCAGGA GAAGTGGCAG GACAGCCGGC GCTGGACGCT CAGCACAGCC 
ACACAGCTGC CGGAGCTGGA GCAGAGTCTG CGGCCCAACT CGGGCTTCGG GAACAGCGGT 
GGGTTCGGGA ACAGATCAGG ATTCGGGAAT CGCTCTGGGA ATCGTTCAGG ATTCGGAAAC 
CGTGGAGGAC GAGGACGATC ATCCGGCTTC AGGAGCTCAG GACGCGGTGG AGGAGGAGGA 
CAGAAACGCA GCTTCAGTCG GGCCTTCAAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120807.2
CDS	6..2381
Translation	MRTSALRTSASVLSFPHVCCESSMSSRSSTSSMPSKSLPESEAERDHLMEDIHNGLQLLKKKKKKLKEQQQTAETDECESPAPKKRKMKISSDTTDLNGNTDTHTLKVKKKKIKLEEEEEETTSTLLEKKKKKKIKLEKEEMMEETNTLLEKKKKIKMEKEEEKMMNGDSHTHSTEPAASSTDSDSEQEKETPEQREGAFSNFRISPNTIKLLQARGVSYLFDIQVKTFNAVYDGKDLIGQARTGTGKTFSFAVPLVEKLQSGDQERRRGRPPKVLVLAPTRELAIQVTKDFKDITRKLSVTCFYGGSSYNPQIDAIRSGIDVLVGTPGRIRDHLQNNKLDLSQLQHVVLDEVDQMLDMGFAEQVEEILSASYKKDAEQKPQTLLFSATCPSWVYDVAKKYMRSQFIHVDLIGKKTQKAATTVEHLAIACHWSQRASVIGDVIQVYSGSHGRTIVFCETKKEATELSLNTSIKQSAQSLHGDIPQKQREVTLKGFRNGSFEVLVATNVAARGLDIPEVDLVIQSSPPNDVESYIHRSGRTGRAGRTGICICFYQRKEESQLKFVEQKAGITFKRVGVPTAYDIIQSSSKDAVRFLDSVPAVAVGYFREAAQELIQQRGAVEALAAALAHISGATSLEQRSLINSEPGFSSMILSCSQEMHSISFAWRGLKEQLGEEIDQHISHMVFIKGKKGVCFDVPVSKLKEVQEKWQDSRRWTLSTATQLPELEQSLRPNSGFGNSGGFGNRSGFGNRSGNRSGFGNRGGRGRSSGFRSSGRGGGGGQKRSFSRAFNY

Target ORF information:

RefSeq Version	NM_001127335.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127335.2

ORF Insert Sequence:

ATGCGCACTT CCGCTTTGCG CACGTCCGCT TCCGTCCTTT CCTTTCCTCA CGTGTGTTGT 
GAGAGCTCCA TGTCCAGCCG CTCCTCAACA TCCAGCATGC CGTCAAAGAG TCTCCCAGAA 
TCGGAAGCCG AGAGAGACCA CCTGATGGAG GACATTCACA ACGGCCTGCA GCTGCTCAAG 
AAGAAGAAGA AGAAGCTGAA GGAGCAGCAG CAGACGGCAG AAACAGACGA GTGTGAGTCT 
CCTGCTCCTA AAAAGAGGAA GATGAAGATT TCCAGCGACA CCACAGACCT GAACGGAAAC 
ACAGACACAC ACACACTCAA GGTCAAGAAG AAGAAGATCA AGCTGGAGGA GGAGGAGGAG 
GAGACGACGA GCACTCTGCT GGAGAAGAAG AAGAAGAAGA AGATCAAGCT GGAGAAGGAG 
GAGATGATGG AGGAGACGAA CACTCTGCTG GAGAAGAAGA AGAAGATCAA GATGGAGAAA 
GAGGAGGAGA AGATGATGAA CGGAGACTCA CACACACACT CCACAGAGCC TGCAGCGTCC 
AGCACAGACT CCGACAGCGA ACAGGAAAAA GAGACTCCAG AGCAGAGAGA AGGAGCTTTC 
TCCAACTTCA GGATCTCACC GAACACCATC AAACTGCTGC AGGCTCGTGG CGTCTCGTAC 
CTGTTTGACA TCCAGGTGAA GACGTTTAAT GCAGTGTATG ATGGGAAGGA TCTGATTGGT 
CAGGCCCGGA CCGGCACCGG GAAGACCTTC TCCTTCGCTG TTCCTCTGGT GGAGAAGCTG 
CAGTCTGGAG ACCAGGAGCG GCGGAGAGGA CGACCACCAA AGGTTCTGGT TCTGGCTCCG 
ACTCGTGAGC TGGCCATACA GGTGACCAAA GACTTCAAGG ACATCACCAG GAAACTCTCC 
GTCACCTGCT TCTATGGAGG AAGCTCATAC AACCCTCAGA TCGATGCGAT CCGCAGTGGC 
ATTGACGTCC TGGTGGGGAC GCCGGGCCGA ATCCGAGACC ATCTGCAGAA CAATAAGCTG 
GATCTGTCTC AGCTGCAGCA TGTGGTGCTG GACGAGGTGG ACCAGATGCT GGACATGGGC 
TTCGCTGAGC AGGTGGAGGA GATCCTGTCT GCCTCCTACA AGAAAGATGC GGAGCAGAAG 
CCGCAGACGC TGCTGTTCTC CGCCACGTGT CCCTCATGGG TTTATGACGT GGCCAAGAAA 
TACATGCGCT CACAGTTCAT CCACGTGGAC CTCATCGGGA AGAAGACACA GAAAGCTGCA 
ACCACTGTCG AGCACCTGGC CATCGCATGT CACTGGTCTC AGAGGGCGTC GGTCATCGGT 
GATGTCATCC AGGTGTACAG CGGCAGCCAC GGCCGCACCA TCGTCTTCTG TGAGACCAAG 
AAGGAGGCGA CAGAGCTGTC CCTGAACACC TCCATCAAAC AGAGTGCTCA GTCTCTGCAT 
GGAGATATCC CGCAGAAGCA GAGGGAGGTG ACGCTGAAGG GTTTCAGGAA CGGCTCTTTC 
GAGGTGTTGG TGGCCACTAA TGTAGCTGCG CGCGGGCTGG ACATCCCAGA GGTGGACCTG 
GTCATCCAGA GCTCACCACC AAACGATGTG GAGTCGTATA TCCACCGGTC GGGCCGGACG 
GGCAGAGCTG GAAGAACCGG CATCTGCATC TGCTTCTACC AGCGCAAAGA GGAGAGCCAG 
CTGAAGTTTG TGGAGCAGAA AGCGGGGATC ACCTTTAAGC GAGTGGGCGT TCCCACAGCC 
TATGACATCA TCCAGTCCTC CAGTAAAGAC GCCGTCAGGT TTCTGGACTC GGTGCCGGCA 
GTAGCGGTGG GATATTTCCG TGAGGCGGCG CAGGAGCTGA TCCAGCAGCG GGGGGCAGTG 
GAGGCTCTGG CAGCAGCACT CGCTCACATA TCAGGAGCCA CCAGCCTGGA GCAGAGATCC 
CTGATCAACT CTGAGCCTGG TTTCTCCAGC ATGATCCTCA GCTGCTCGCA GGAGATGCAC 
AGCATAAGTT TCGCCTGGAG AGGACTGAAG GAGCAGCTGG GAGAGGAGAT AGACCAGCAC 
ATCTCACACA TGGTCTTCAT CAAGGGGAAG AAGGGTGTGT GTTTCGACGT CCCAGTCAGC 
AAACTGAAGG AGGTCCAGGA GAAGTGGCAG GACAGCCGGC GCTGGACGCT CAGCACAGCC 
ACACAGCTGC CGGAGCTGGA GCAGAGTCTG CGGCCCAACT CGGGCTTCGG GAACAGCGGT 
GGGTTCGGGA ACAGATCAGG ATTCGGGAAT CGCTCTGGGA ATCGTTCAGG ATTCGGAAAC 
CGTGGAGGAC GAGGACGATC ATCCGGCTTC AGGAGCTCAG GACGCGGTGG AGGAGGAGGA 
CAGAAACGCA GCTTCAGTCG GGCCTTCAAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx21 ( XM_017358815.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214304.1 Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54749
Clone ID Related Accession (Same CDS sequence)	XM_017358815.1
Accession Version	XM_017358815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolar RNA helicase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCACTT CCGCTTTGCG CACGTCCGCT TCCGTCCTTT CCTTTCCTCA CGTGTGTTGT 
GAGAGCTCCA TGTCCAGCCG CTCCTCAACA TCCAGCATGC CGTCAAAGAG TCTCCCAGAA 
TCGGAAGCCG AGAGAGACCA CCTGATGGAG GACATTCACA ACGGCCTGCA GCTGCTCAAG 
AAGAAGAAGA AGCTGAAGGA GCAGCAGCAG ACGGCAGAAA CAGACGAGTG TGAGTCTCCT 
GCTCCTAAAA AGAGGAAGAT GAAGATTTCC AGCGACACCA CAGACCTGAA CGGAAACACA 
GACACACACA CACTCAAGGT CAAGAAGAAG AAGATCAAGC TGGAGGAGGA GGAGGAGGAG 
ACGACGAGCA CTCTGCTGGA GAAGAAGAAG AAGAAGAAGA TCAAGCTGGA GAAGGAGGAG 
ATGATGGAGG AGACGAACAC TCTGCTGGAG AAGAAGAAGA AGATCAAGAT GGAGAAAGAG 
GAGGAGAAGA TGATGAACGG AGACTCACAC ACACACTCCA CAGAGCCTGC AGCGTCCAGC 
ACAGACTCCG ACAGCGAACA GGAAAAAGAG ACTCCAGAGC AGAGAGAAGG AGCTTTCTCC 
AACTTCAGGA TCTCACCGAA CACCATCAAA CTGCTGCAGG CTCGTGGCGT CTCGTACCTG 
TTTGACATCC AGGTGAAGAC GTTTAATGCA GTGTATGATG GGAAGGATCT GATTGGTCAG 
GCCCGGACCG GCACCGGGAA GACCTTCTCC TTCGCTGTTC CTCTGGTGGA GAAGCTGCAG 
TCTGGAGACC AGGAGCGGCG GAGAGGACGA CCACCAAAGG TTCTGGTTCT GGCTCCGACT 
CGTGAGCTGG CCATACAGGT GACCAAAGAC TTCAAGGACA TCACCAGGAA ACTCTCCGTC 
ACCTGCTTCT ATGGAGGAAG CTCATACAAC CCTCAGATCG ATGCGATCCG CAGTGGCATT 
GACGTCCTGG TGGGGACGCC GGGCCGAATC CGAGACCATC TGCAGAACAA TAAGCTGGAT 
CTGTCTCAGC TGCAGCATGT GGTGCTGGAC GAGGTGGACC AGATGCTGGA CATGGGCTTC 
GCTGAGCAGG TGGAGGAGAT CCTGTCTGCC TCCTACAAGA AAGATGCGGA GCAGAAGCCG 
CAGACGCTGC TGTTCTCCGC CACGTGTCCC TCATGGGTTT ATGACGTGGC CAAGAAATAC 
ATGCGCTCAC AGTTCATCCA CGTGGACCTC ATCGGGAAGA AGACACAGAA AGCTGCAACC 
ACTGTCGAGC ACCTGGCCAT CGCATGTCAC TGGTCTCAGA GGGCGTCGGT CATCGGTGAT 
GTCATCCAGG TGTACAGCGG CAGCCACGGC CGCACCATCG TCTTCTGTGA GACCAAGAAG 
GAGGCGACAG AGCTGTCCCT GAACACCTCC ATCAAACAGA GTGCTCAGTC TCTGCATGGA 
GATATCCCGC AGAAGCAGAG GGAGGTGACG CTGAAGGGTT TCAGGAACGG CTCTTTCGAG 
GTGTTGGTGG CCACTAATGT AGCTGCGCGC GGGCTGGACA TCCCAGAGGT GGACCTGGTC 
ATCCAGAGCT CACCACCAAA CGATGTGGAG TCGTATATCC ACCGGTCGGG CCGGACGGGC 
AGAGCTGGAA GAACCGGCAT CTGCATCTGC TTCTACCAGC GCAAAGAGGA GAGCCAGCTG 
AAGTTTGTGG AGCAGAAAGC GGGGATCACC TTTAAGCGAG TGGGCGTTCC CACAGCCTAT 
GACATCATCC AGTCCTCCAG TAAAGACGCC GTCAGGTTTC TGGACTCGGT GCCGGCAGTA 
GCGGTGGGAT ATTTCCGTGA GGCGGCGCAG GAGCTGATCC AGCAGCGGGG GGCAGTGGAG 
GCTCTGGCAG CAGCACTCGC TCACATATCA GGAGCCACCA GCCTGGAGCA GAGATCCCTG 
ATCAACTCTG AGCCTGGTTT CTCCAGCATG ATCCTCAGCT GCTCGCAGGA GATGCACAGC 
ATAAGTTTCG CCTGGAGAGG ACTGAAGGAG CAGCTGGGAG AGGAGATAGA CCAGCACATC 
TCACACATGG TCTTCATCAA GGGGAAGAAG GGTGTGTGTT TCGACGTCCC AGTCAGCAAA 
CTGAAGGAGG TCCAGGAGAA GTGGCAGGAC AGCCGGCGCT GGACGCTCAG CACAGCCACA 
CAGCTGCCGG AGCTGGAGCA GAGTCTGCGG CCCAACTCGG GCTTCGGGAA CAGCGGTGGG 
TTCGGGAACA GATCAGGATT CGGGAATCGC TCTGGGAATC GTTCAGGATT CGGAAACCGT 
GGAGGACGAG GACGATCATC CGGCTTCAGG AGCTCAGGAC GCGGTGGAGG AGGAGGACAG 
AAACGCAGCT TCAGTCGGGC CTTCAACTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214304.1
CDS	8..2380
Translation	MRTSALRTSASVLSFPHVCCESSMSSRSSTSSMPSKSLPESEAERDHLMEDIHNGLQLLKKKKKLKEQQQTAETDECESPAPKKRKMKISSDTTDLNGNTDTHTLKVKKKKIKLEEEEEETTSTLLEKKKKKKIKLEKEEMMEETNTLLEKKKKIKMEKEEEKMMNGDSHTHSTEPAASSTDSDSEQEKETPEQREGAFSNFRISPNTIKLLQARGVSYLFDIQVKTFNAVYDGKDLIGQARTGTGKTFSFAVPLVEKLQSGDQERRRGRPPKVLVLAPTRELAIQVTKDFKDITRKLSVTCFYGGSSYNPQIDAIRSGIDVLVGTPGRIRDHLQNNKLDLSQLQHVVLDEVDQMLDMGFAEQVEEILSASYKKDAEQKPQTLLFSATCPSWVYDVAKKYMRSQFIHVDLIGKKTQKAATTVEHLAIACHWSQRASVIGDVIQVYSGSHGRTIVFCETKKEATELSLNTSIKQSAQSLHGDIPQKQREVTLKGFRNGSFEVLVATNVAARGLDIPEVDLVIQSSPPNDVESYIHRSGRTGRAGRTGICICFYQRKEESQLKFVEQKAGITFKRVGVPTAYDIIQSSSKDAVRFLDSVPAVAVGYFREAAQELIQQRGAVEALAAALAHISGATSLEQRSLINSEPGFSSMILSCSQEMHSISFAWRGLKEQLGEEIDQHISHMVFIKGKKGVCFDVPVSKLKEVQEKWQDSRRWTLSTATQLPELEQSLRPNSGFGNSGGFGNRSGFGNRSGNRSGFGNRGGRGRSSGFRSSGRGGGGGQKRSFSRAFNY

Target ORF information:

RefSeq Version	XM_017358815.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358815.1

ORF Insert Sequence:

ATGCGCACTT CCGCTTTGCG CACGTCCGCT TCCGTCCTTT CCTTTCCTCA CGTGTGTTGT 
GAGAGCTCCA TGTCCAGCCG CTCCTCAACA TCCAGCATGC CGTCAAAGAG TCTCCCAGAA 
TCGGAAGCCG AGAGAGACCA CCTGATGGAG GACATTCACA ACGGCCTGCA GCTGCTCAAG 
AAGAAGAAGA AGCTGAAGGA GCAGCAGCAG ACGGCAGAAA CAGACGAGTG TGAGTCTCCT 
GCTCCTAAAA AGAGGAAGAT GAAGATTTCC AGCGACACCA CAGACCTGAA CGGAAACACA 
GACACACACA CACTCAAGGT CAAGAAGAAG AAGATCAAGC TGGAGGAGGA GGAGGAGGAG 
ACGACGAGCA CTCTGCTGGA GAAGAAGAAG AAGAAGAAGA TCAAGCTGGA GAAGGAGGAG 
ATGATGGAGG AGACGAACAC TCTGCTGGAG AAGAAGAAGA AGATCAAGAT GGAGAAAGAG 
GAGGAGAAGA TGATGAACGG AGACTCACAC ACACACTCCA CAGAGCCTGC AGCGTCCAGC 
ACAGACTCCG ACAGCGAACA GGAAAAAGAG ACTCCAGAGC AGAGAGAAGG AGCTTTCTCC 
AACTTCAGGA TCTCACCGAA CACCATCAAA CTGCTGCAGG CTCGTGGCGT CTCGTACCTG 
TTTGACATCC AGGTGAAGAC GTTTAATGCA GTGTATGATG GGAAGGATCT GATTGGTCAG 
GCCCGGACCG GCACCGGGAA GACCTTCTCC TTCGCTGTTC CTCTGGTGGA GAAGCTGCAG 
TCTGGAGACC AGGAGCGGCG GAGAGGACGA CCACCAAAGG TTCTGGTTCT GGCTCCGACT 
CGTGAGCTGG CCATACAGGT GACCAAAGAC TTCAAGGACA TCACCAGGAA ACTCTCCGTC 
ACCTGCTTCT ATGGAGGAAG CTCATACAAC CCTCAGATCG ATGCGATCCG CAGTGGCATT 
GACGTCCTGG TGGGGACGCC GGGCCGAATC CGAGACCATC TGCAGAACAA TAAGCTGGAT 
CTGTCTCAGC TGCAGCATGT GGTGCTGGAC GAGGTGGACC AGATGCTGGA CATGGGCTTC 
GCTGAGCAGG TGGAGGAGAT CCTGTCTGCC TCCTACAAGA AAGATGCGGA GCAGAAGCCG 
CAGACGCTGC TGTTCTCCGC CACGTGTCCC TCATGGGTTT ATGACGTGGC CAAGAAATAC 
ATGCGCTCAC AGTTCATCCA CGTGGACCTC ATCGGGAAGA AGACACAGAA AGCTGCAACC 
ACTGTCGAGC ACCTGGCCAT CGCATGTCAC TGGTCTCAGA GGGCGTCGGT CATCGGTGAT 
GTCATCCAGG TGTACAGCGG CAGCCACGGC CGCACCATCG TCTTCTGTGA GACCAAGAAG 
GAGGCGACAG AGCTGTCCCT GAACACCTCC ATCAAACAGA GTGCTCAGTC TCTGCATGGA 
GATATCCCGC AGAAGCAGAG GGAGGTGACG CTGAAGGGTT TCAGGAACGG CTCTTTCGAG 
GTGTTGGTGG CCACTAATGT AGCTGCGCGC GGGCTGGACA TCCCAGAGGT GGACCTGGTC 
ATCCAGAGCT CACCACCAAA CGATGTGGAG TCGTATATCC ACCGGTCGGG CCGGACGGGC 
AGAGCTGGAA GAACCGGCAT CTGCATCTGC TTCTACCAGC GCAAAGAGGA GAGCCAGCTG 
AAGTTTGTGG AGCAGAAAGC GGGGATCACC TTTAAGCGAG TGGGCGTTCC CACAGCCTAT 
GACATCATCC AGTCCTCCAG TAAAGACGCC GTCAGGTTTC TGGACTCGGT GCCGGCAGTA 
GCGGTGGGAT ATTTCCGTGA GGCGGCGCAG GAGCTGATCC AGCAGCGGGG GGCAGTGGAG 
GCTCTGGCAG CAGCACTCGC TCACATATCA GGAGCCACCA GCCTGGAGCA GAGATCCCTG 
ATCAACTCTG AGCCTGGTTT CTCCAGCATG ATCCTCAGCT GCTCGCAGGA GATGCACAGC 
ATAAGTTTCG CCTGGAGAGG ACTGAAGGAG CAGCTGGGAG AGGAGATAGA CCAGCACATC 
TCACACATGG TCTTCATCAA GGGGAAGAAG GGTGTGTGTT TCGACGTCCC AGTCAGCAAA 
CTGAAGGAGG TCCAGGAGAA GTGGCAGGAC AGCCGGCGCT GGACGCTCAG CACAGCCACA 
CAGCTGCCGG AGCTGGAGCA GAGTCTGCGG CCCAACTCGG GCTTCGGGAA CAGCGGTGGG 
TTCGGGAACA GATCAGGATT CGGGAATCGC TCTGGGAATC GTTCAGGATT CGGAAACCGT 
GGAGGACGAG GACGATCATC CGGCTTCAGG AGCTCAGGAC GCGGTGGAGG AGGAGGACAG 
AAACGCAGCT TCAGTCGGGC CTTCAACTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Investigation of the effects of estrogen on skeletal gene expression during zebrafish larval head development.
PeerJ4e1878(2016)
Pashay Ahi E,Walker BS,Lassiter CS,J?nsson ZO

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)
Feng H,Zhang YB,Zhang QM,Li Z,Zhang QY,Gui JF

TET2 plays an essential role in erythropoiesis by regulating lineage-specific genes via DNA oxidative demethylation in a zebrafish model.
Molecular and cellular biology34(6)989-1002(2014 Mar)
Ge L,Zhang RP,Wan F,Guo DY,Wang P,Xiang LX,Shao JZ