SPNS1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Gene Symbol	SPNS1
Entrez Gene ID	799873
Full Name	spinster homolog 1 (Drosophila)
Synonyms	etID64740.3,nrs,spinl,wu:fb95b12,wu:fi37e11
General protein information	Preferred Names spinster homolog 1 (Drosophila) Names protein spinster homolog 1 not really started protein not really started spinster-like protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163832.4	XP_005163889.1	protein spinster homolog 1 isoform X1
NM_153663.2	NP_705949.2	protein spinster homolog 1
XM_005163832.3	XP_005163889.1	protein spinster homolog 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPNS1	XP_003639185.2
Bos taurus (cattle)	SPNS1	NP_001070276.1
Drosophila melanogaster (fruit fly)	spin	NP_725530.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006346	XP_316366.4
Caenorhabditis elegans (roundworm)	F09A5.1	NP_510181.3
Mus musculus (house mouse)	Spns1	NP_076201.2
Danio rerio (zebrafish)	spns1	NP_705949.1
	SPNS1	XP_001102328.2
Caenorhabditis elegans (roundworm)	CELE_C39E9.10	NP_502513.1
Arabidopsis thaliana (thale cress)	AT5G65687	NP_680469.1
Arabidopsis thaliana (thale cress)	AT2G22730	NP_179858.2
Arabidopsis thaliana (thale cress)	AT5G64500	NP_201255.1
Xenopus tropicalis (tropical clawed frog)	spns1	NP_001106495.1
Homo sapiens (human)	SPNS1	NP_114427.1
Pan troglodytes (chimpanzee)	SPNS1	XP_001147131.2
Caenorhabditis elegans (roundworm)	C13C4.5	NP_506041.1
Rattus norvegicus (Norway rat)	Spns1	NP_001034297.2
Caenorhabditis elegans (roundworm)	CELE_Y111B2A.19	NP_499650.2

Gene Ontology
Bibliography

Related articles in PubMed

Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase.
Sasaki T, Lian S, Khan A, Llop JR, Samuelson AV, Chen W, Klionsky DJ, Kishi S
Autophagy13(2)386-403(2017 Feb)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Aberrant autolysosomal regulation is linked to the induction of embryonic senescence: differential roles of Beclin 1 and p53 in vertebrate Spns1 deficiency.
Sasaki T, Lian S, Qi J, Bayliss PE, Carr CE, Johnson JL, Guha S, Kobler P, Catz SD, Gill M, Jia K, Klionsky DJ, Kishi S
PLoS genetics10(6)e1004409(2014 Jun)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.
Young RM, Marty S, Nakano Y, Wang H, Yamamoto D, Lin S, Allende ML
Developmental dynamics : an official publication of the American Association of Anatomists223(2)298-305(2002 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spns1 and the v-ATPase orchestrate proper autolysosomal biogenesis with optimal acidification that is critically linked to developmental senescence and survival.
Title: Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase.

Spns1 operates during autophagy and senescence differentially with Beclin 1 and p53.
Title: Aberrant autolysosomal regulation is linked to the induction of embryonic senescence: differential roles of Beclin 1 and p53 in vertebrate Spns1 deficiency.

Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.
Title: Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.

The following SPNS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPNS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08104	NM_153663.2 Latest version!	Danio rerio spinster homolog 1 (Drosophila) (spns1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa08104	XM_005163832.4 Latest version!	Danio rerio spinster homolog 1 (Drosophila) (spns1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa08104	XM_005163832.3	Danio rerio spinster homolog 1 (Drosophila) (spns1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPNS1 ( NM_153663.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_705949.2 Danio rerio spinster homolog 1 (Drosophila) (spns1), mRNA.

Price & Availability↑

CloneID	ODa08104
Clone ID Related Accession (Same CDS sequence)	XM_005163832.4 , NM_153663.2 , XM_005163832.3
Accession Version	NM_153663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Danio rerio(zebrafish)
Product	protein spinster homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH595239.1, CK029141.1, EH585608.1, CK027241.1, BX784026.17, CF999294.1 and EH478886.1. On Sep 3, 2015 this sequence version replaced NM_153663.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF465772.1, BC048024.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG 
CCTGTTGAAA ATGGGGTGGG GTCACCTCTA CCTGAGGACG AGGAGTCTCC CAGCGGAGTC 
ACCGACAGGA GAGCCATAAT GACTGTGATT GTGCTCTGCT ACATCAACCT GCTGAACTAT 
ATGGACAGAT TCACTGTGGC AGGTGTACTT CCAGACATTG AACATTTCTT CGGCATTGGT 
GATGGAACGT CAGGGCTGCT TCAAACAGTC TTCATCTGCA GCTACATGTT CTTGGCTCCT 
CTATTCGGAT ACCTGGGTGA CCGGTATAAT AGGAAGCTGA TCATGTGTGT GGGGATTTTC 
TTCTGGTCTG TGGTAACACT TGCCAGCTCA TTTATCGGGA AAGATCACTT CTGGGCACTG 
CTGTTGACAC GAGGGCTAGT GGGTGTTGGA GAGGCCAGTT ATTCCACCAT TGCTCCCACC 
ATCATCGCTG ACCTCTTTGT CAAGGAAAAG AGGACTAACA TGCTGTCTAT ATTTTATTTT 
GCCATCCCAG TGGGCAGTGG TATGGGTTAT ATCGTGGGCT CAAAGGTGGA TACTGTGGCT 
AAAGATTGGC ATTGGGCACT TCGGGTGACC CCTGGGCTGG GACTGCTGGC CGTGTTCCTC 
TTAATGCTTG TGGTTCAGGA ACCGAAGCGT GGTGCGATTG AAGCTCACCC CGAGCACACG 
CTGCACAGAA CCAGCTGGCT GGCAGACATG AAAGCACTTT GCAGGAATCC AAGTTTTATT 
CTGTCCACGT TCGGCTTCAC GGCAGTGGCC TTTGTGACGG GATCACTGGC CCTGTGGGCT 
CCTGCCTTTT TATTTAGAGC CGGCGTCTTC ACCGGAGTCA AGCAGCCGTG TTTTAAAGCT 
CCATGTGATG ACTCTGACAG TCTGATTTTT GGCGCCATCA CTGTAGTAAC GGGCATCTTG 
GGTGTAGCGA GCGGTGTTCA GGCCAGTAAA CTGCTGAGGA CCAGAACTCC TCGAGCTGAC 
CCGCTAGTGT GTGCAGCTGG ACTCCTCCTC GCGGCTCCCT TTCTCTATCT CTCCATCATG 
TTTGCTCAGG CGAGCACTGT GGCCACATAT GTCTTTATCT TCCTGGGAGA GACATTCCTT 
TCAATGAATT GGGCCATTGT TGCTGATATC CTACTGTATG TTGTGATCCC GACACGTCGC 
TCCACAGCAG AGGCCTTTCA GATTGTCCTT TCACACTTGC TGGGTGACGC TATTAGTCCG 
TATCTCATAG GAGTGGTTTC AGACTCAATA AAAGAATCAA ATTCGTACAT GTGGGAGTTT 
CGCTCACTTC AGATGTCTCT GCTGCTGTGC TCTTTCGTGG CCGTCGCTGG AGGCGCTTTC 
TTCTTGGCCA CAGCCGTCTT TATTGAGAAA GACCGAGATC TCGCTGAGAA CTATGTGCCC 
TCTGATGACG CACCAATTGT TGTTCCAAGG AGCGGCAGAT CCACTAAAGT ATCAGTGTCG 
AGTGTTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705949.2
CDS	241..1755
Translation	MSQADADITPFFADDNEGEGPVENGVGSPLPEDEESPSGVTDRRAIMTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSLIFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKESNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDRDLAENYVPSDDAPIVVPRSGRSTKVSVSSVLI

Target ORF information:

RefSeq Version	NM_153663.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spinster homolog 1 (Drosophila) (spns1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153663.2

ORF Insert Sequence:

ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG 
CCTGTTGAAA ATGGGGTGGG GTCACCTCTA CCTGAGGACG AGGAGTCTCC CAGCGGAGTC 
ACCGACAGGA GAGCCATAAT GACTGTGATT GTGCTCTGCT ACATCAACCT GCTGAACTAT 
ATGGACAGAT TCACTGTGGC AGGTGTACTT CCAGACATTG AACATTTCTT CGGCATTGGT 
GATGGAACGT CAGGGCTGCT TCAAACAGTC TTCATCTGCA GCTACATGTT CTTGGCTCCT 
CTATTCGGAT ACCTGGGTGA CCGGTATAAT AGGAAGCTGA TCATGTGTGT GGGGATTTTC 
TTCTGGTCTG TGGTAACACT TGCCAGCTCA TTTATCGGGA AAGATCACTT CTGGGCACTG 
CTGTTGACAC GAGGGCTAGT GGGTGTTGGA GAGGCCAGTT ATTCCACCAT TGCTCCCACC 
ATCATCGCTG ACCTCTTTGT CAAGGAAAAG AGGACTAACA TGCTGTCTAT ATTTTATTTT 
GCCATCCCAG TGGGCAGTGG TATGGGTTAT ATCGTGGGCT CAAAGGTGGA TACTGTGGCT 
AAAGATTGGC ATTGGGCACT TCGGGTGACC CCTGGGCTGG GACTGCTGGC CGTGTTCCTC 
TTAATGCTTG TGGTTCAGGA ACCGAAGCGT GGTGCGATTG AAGCTCACCC CGAGCACACG 
CTGCACAGAA CCAGCTGGCT GGCAGACATG AAAGCACTTT GCAGGAATCC AAGTTTTATT 
CTGTCCACGT TCGGCTTCAC GGCAGTGGCC TTTGTGACGG GATCACTGGC CCTGTGGGCT 
CCTGCCTTTT TATTTAGAGC CGGCGTCTTC ACCGGAGTCA AGCAGCCGTG TTTTAAAGCT 
CCATGTGATG ACTCTGACAG TCTGATTTTT GGCGCCATCA CTGTAGTAAC GGGCATCTTG 
GGTGTAGCGA GCGGTGTTCA GGCCAGTAAA CTGCTGAGGA CCAGAACTCC TCGAGCTGAC 
CCGCTAGTGT GTGCAGCTGG ACTCCTCCTC GCGGCTCCCT TTCTCTATCT CTCCATCATG 
TTTGCTCAGG CGAGCACTGT GGCCACATAT GTCTTTATCT TCCTGGGAGA GACATTCCTT 
TCAATGAATT GGGCCATTGT TGCTGATATC CTACTGTATG TTGTGATCCC GACACGTCGC 
TCCACAGCAG AGGCCTTTCA GATTGTCCTT TCACACTTGC TGGGTGACGC TATTAGTCCG 
TATCTCATAG GAGTGGTTTC AGACTCAATA AAAGAATCAA ATTCGTACAT GTGGGAGTTT 
CGCTCACTTC AGATGTCTCT GCTGCTGTGC TCTTTCGTGG CCGTCGCTGG AGGCGCTTTC 
TTCTTGGCCA CAGCCGTCTT TATTGAGAAA GACCGAGATC TCGCTGAGAA CTATGTGCCC 
TCTGATGACG CACCAATTGT TGTTCCAAGG AGCGGCAGAT CCACTAAAGT ATCAGTGTCG 
AGTGTTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPNS1 ( XM_005163832.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163889.1 Danio rerio spinster homolog 1 (Drosophila) (spns1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08104
Clone ID Related Accession (Same CDS sequence)	XM_005163832.4 , NM_153663.2 , XM_005163832.3
Accession Version	XM_005163832.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein spinster homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG 
CCTGTTGAAA ATGGGGTGGG GTCACCTCTA CCTGAGGACG AGGAGTCTCC CAGCGGAGTC 
ACCGACAGGA GAGCCATAAT GACTGTGATT GTGCTCTGCT ACATCAACCT GCTGAACTAT 
ATGGACAGAT TCACTGTGGC AGGTGTACTT CCAGACATTG AACATTTCTT CGGCATTGGT 
GATGGAACGT CAGGGCTGCT TCAAACAGTC TTCATCTGCA GCTACATGTT CTTGGCTCCT 
CTATTCGGAT ACCTGGGTGA CCGGTATAAT AGGAAGCTGA TCATGTGTGT GGGGATTTTC 
TTCTGGTCTG TGGTAACACT TGCCAGCTCA TTTATCGGGA AAGATCACTT CTGGGCACTG 
CTGTTGACAC GAGGGCTAGT GGGTGTTGGA GAGGCCAGTT ATTCCACCAT TGCTCCCACC 
ATCATCGCTG ACCTCTTTGT CAAGGAAAAG AGGACTAACA TGCTGTCTAT ATTTTATTTT 
GCCATCCCAG TGGGCAGTGG TATGGGTTAT ATCGTGGGCT CAAAGGTGGA TACTGTGGCT 
AAAGATTGGC ATTGGGCACT TCGGGTGACC CCTGGGCTGG GACTGCTGGC CGTGTTCCTC 
TTAATGCTTG TGGTTCAGGA ACCGAAGCGT GGTGCGATTG AAGCTCACCC CGAGCACACG 
CTGCACAGAA CCAGCTGGCT GGCAGACATG AAAGCACTTT GCAGGAATCC AAGTTTTATT 
CTGTCCACGT TCGGCTTCAC GGCAGTGGCC TTTGTGACGG GATCACTGGC CCTGTGGGCT 
CCTGCCTTTT TATTTAGAGC CGGCGTCTTC ACCGGAGTCA AGCAGCCGTG TTTTAAAGCT 
CCATGTGATG ACTCTGACAG TCTGATTTTT GGCGCCATCA CTGTAGTAAC GGGCATCTTG 
GGTGTAGCGA GCGGTGTTCA GGCCAGTAAA CTGCTGAGGA CCAGAACTCC TCGAGCTGAC 
CCGCTAGTGT GTGCAGCTGG ACTCCTCCTC GCGGCTCCCT TTCTCTATCT CTCCATCATG 
TTTGCTCAGG CGAGCACTGT GGCCACATAT GTCTTTATCT TCCTGGGAGA GACATTCCTT 
TCAATGAATT GGGCCATTGT TGCTGATATC CTACTGTATG TTGTGATCCC GACACGTCGC 
TCCACAGCAG AGGCCTTTCA GATTGTCCTT TCACACTTGC TGGGTGACGC TATTAGTCCG 
TATCTCATAG GAGTGGTTTC AGACTCAATA AAAGAATCAA ATTCGTACAT GTGGGAGTTT 
CGCTCACTTC AGATGTCTCT GCTGCTGTGC TCTTTCGTGG CCGTCGCTGG AGGCGCTTTC 
TTCTTGGCCA CAGCCGTCTT TATTGAGAAA GACCGAGATC TCGCTGAGAA CTATGTGCCC 
TCTGATGACG CACCAATTGT TGTTCCAAGG AGCGGCAGAT CCACTAAAGT ATCAGTGTCG 
AGTGTTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163889.1
CDS	255..1769
Translation	MSQADADITPFFADDNEGEGPVENGVGSPLPEDEESPSGVTDRRAIMTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSLIFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKESNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDRDLAENYVPSDDAPIVVPRSGRSTKVSVSSVLI

Target ORF information:

RefSeq Version	XM_005163832.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spinster homolog 1 (Drosophila) (spns1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163832.4

ORF Insert Sequence:

ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG 
CCTGTTGAAA ATGGGGTGGG GTCACCTCTA CCTGAGGACG AGGAGTCTCC CAGCGGAGTC 
ACCGACAGGA GAGCCATAAT GACTGTGATT GTGCTCTGCT ACATCAACCT GCTGAACTAT 
ATGGACAGAT TCACTGTGGC AGGTGTACTT CCAGACATTG AACATTTCTT CGGCATTGGT 
GATGGAACGT CAGGGCTGCT TCAAACAGTC TTCATCTGCA GCTACATGTT CTTGGCTCCT 
CTATTCGGAT ACCTGGGTGA CCGGTATAAT AGGAAGCTGA TCATGTGTGT GGGGATTTTC 
TTCTGGTCTG TGGTAACACT TGCCAGCTCA TTTATCGGGA AAGATCACTT CTGGGCACTG 
CTGTTGACAC GAGGGCTAGT GGGTGTTGGA GAGGCCAGTT ATTCCACCAT TGCTCCCACC 
ATCATCGCTG ACCTCTTTGT CAAGGAAAAG AGGACTAACA TGCTGTCTAT ATTTTATTTT 
GCCATCCCAG TGGGCAGTGG TATGGGTTAT ATCGTGGGCT CAAAGGTGGA TACTGTGGCT 
AAAGATTGGC ATTGGGCACT TCGGGTGACC CCTGGGCTGG GACTGCTGGC CGTGTTCCTC 
TTAATGCTTG TGGTTCAGGA ACCGAAGCGT GGTGCGATTG AAGCTCACCC CGAGCACACG 
CTGCACAGAA CCAGCTGGCT GGCAGACATG AAAGCACTTT GCAGGAATCC AAGTTTTATT 
CTGTCCACGT TCGGCTTCAC GGCAGTGGCC TTTGTGACGG GATCACTGGC CCTGTGGGCT 
CCTGCCTTTT TATTTAGAGC CGGCGTCTTC ACCGGAGTCA AGCAGCCGTG TTTTAAAGCT 
CCATGTGATG ACTCTGACAG TCTGATTTTT GGCGCCATCA CTGTAGTAAC GGGCATCTTG 
GGTGTAGCGA GCGGTGTTCA GGCCAGTAAA CTGCTGAGGA CCAGAACTCC TCGAGCTGAC 
CCGCTAGTGT GTGCAGCTGG ACTCCTCCTC GCGGCTCCCT TTCTCTATCT CTCCATCATG 
TTTGCTCAGG CGAGCACTGT GGCCACATAT GTCTTTATCT TCCTGGGAGA GACATTCCTT 
TCAATGAATT GGGCCATTGT TGCTGATATC CTACTGTATG TTGTGATCCC GACACGTCGC 
TCCACAGCAG AGGCCTTTCA GATTGTCCTT TCACACTTGC TGGGTGACGC TATTAGTCCG 
TATCTCATAG GAGTGGTTTC AGACTCAATA AAAGAATCAA ATTCGTACAT GTGGGAGTTT 
CGCTCACTTC AGATGTCTCT GCTGCTGTGC TCTTTCGTGG CCGTCGCTGG AGGCGCTTTC 
TTCTTGGCCA CAGCCGTCTT TATTGAGAAA GACCGAGATC TCGCTGAGAA CTATGTGCCC 
TCTGATGACG CACCAATTGT TGTTCCAAGG AGCGGCAGAT CCACTAAAGT ATCAGTGTCG 
AGTGTTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPNS1 ( XM_005163832.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163889.1 Danio rerio spinster homolog 1 (Drosophila) (spns1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08104
Clone ID Related Accession (Same CDS sequence)	XM_005163832.4 , NM_153663.2 , XM_005163832.3
Accession Version	XM_005163832.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein spinster homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG 
CCTGTTGAAA ATGGGGTGGG GTCACCTCTA CCTGAGGACG AGGAGTCTCC CAGCGGAGTC 
ACCGACAGGA GAGCCATAAT GACTGTGATT GTGCTCTGCT ACATCAACCT GCTGAACTAT 
ATGGACAGAT TCACTGTGGC AGGTGTACTT CCAGACATTG AACATTTCTT CGGCATTGGT 
GATGGAACGT CAGGGCTGCT TCAAACAGTC TTCATCTGCA GCTACATGTT CTTGGCTCCT 
CTATTCGGAT ACCTGGGTGA CCGGTATAAT AGGAAGCTGA TCATGTGTGT GGGGATTTTC 
TTCTGGTCTG TGGTAACACT TGCCAGCTCA TTTATCGGGA AAGATCACTT CTGGGCACTG 
CTGTTGACAC GAGGGCTAGT GGGTGTTGGA GAGGCCAGTT ATTCCACCAT TGCTCCCACC 
ATCATCGCTG ACCTCTTTGT CAAGGAAAAG AGGACTAACA TGCTGTCTAT ATTTTATTTT 
GCCATCCCAG TGGGCAGTGG TATGGGTTAT ATCGTGGGCT CAAAGGTGGA TACTGTGGCT 
AAAGATTGGC ATTGGGCACT TCGGGTGACC CCTGGGCTGG GACTGCTGGC CGTGTTCCTC 
TTAATGCTTG TGGTTCAGGA ACCGAAGCGT GGTGCGATTG AAGCTCACCC CGAGCACACG 
CTGCACAGAA CCAGCTGGCT GGCAGACATG AAAGCACTTT GCAGGAATCC AAGTTTTATT 
CTGTCCACGT TCGGCTTCAC GGCAGTGGCC TTTGTGACGG GATCACTGGC CCTGTGGGCT 
CCTGCCTTTT TATTTAGAGC CGGCGTCTTC ACCGGAGTCA AGCAGCCGTG TTTTAAAGCT 
CCATGTGATG ACTCTGACAG TCTGATTTTT GGCGCCATCA CTGTAGTAAC GGGCATCTTG 
GGTGTAGCGA GCGGTGTTCA GGCCAGTAAA CTGCTGAGGA CCAGAACTCC TCGAGCTGAC 
CCGCTAGTGT GTGCAGCTGG ACTCCTCCTC GCGGCTCCCT TTCTCTATCT CTCCATCATG 
TTTGCTCAGG CGAGCACTGT GGCCACATAT GTCTTTATCT TCCTGGGAGA GACATTCCTT 
TCAATGAATT GGGCCATTGT TGCTGATATC CTACTGTATG TTGTGATCCC GACACGTCGC 
TCCACAGCAG AGGCCTTTCA GATTGTCCTT TCACACTTGC TGGGTGACGC TATTAGTCCG 
TATCTCATAG GAGTGGTTTC AGACTCAATA AAAGAATCAA ATTCGTACAT GTGGGAGTTT 
CGCTCACTTC AGATGTCTCT GCTGCTGTGC TCTTTCGTGG CCGTCGCTGG AGGCGCTTTC 
TTCTTGGCCA CAGCCGTCTT TATTGAGAAA GACCGAGATC TCGCTGAGAA CTATGTGCCC 
TCTGATGACG CACCAATTGT TGTTCCAAGG AGCGGCAGAT CCACTAAAGT ATCAGTGTCG 
AGTGTTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163889.1
CDS	253..1767
Translation	MSQADADITPFFADDNEGEGPVENGVGSPLPEDEESPSGVTDRRAIMTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSLIFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKESNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDRDLAENYVPSDDAPIVVPRSGRSTKVSVSSVLI

Target ORF information:

RefSeq Version	XM_005163832.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio spinster homolog 1 (Drosophila) (spns1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163832.3

ORF Insert Sequence:

ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG 
CCTGTTGAAA ATGGGGTGGG GTCACCTCTA CCTGAGGACG AGGAGTCTCC CAGCGGAGTC 
ACCGACAGGA GAGCCATAAT GACTGTGATT GTGCTCTGCT ACATCAACCT GCTGAACTAT 
ATGGACAGAT TCACTGTGGC AGGTGTACTT CCAGACATTG AACATTTCTT CGGCATTGGT 
GATGGAACGT CAGGGCTGCT TCAAACAGTC TTCATCTGCA GCTACATGTT CTTGGCTCCT 
CTATTCGGAT ACCTGGGTGA CCGGTATAAT AGGAAGCTGA TCATGTGTGT GGGGATTTTC 
TTCTGGTCTG TGGTAACACT TGCCAGCTCA TTTATCGGGA AAGATCACTT CTGGGCACTG 
CTGTTGACAC GAGGGCTAGT GGGTGTTGGA GAGGCCAGTT ATTCCACCAT TGCTCCCACC 
ATCATCGCTG ACCTCTTTGT CAAGGAAAAG AGGACTAACA TGCTGTCTAT ATTTTATTTT 
GCCATCCCAG TGGGCAGTGG TATGGGTTAT ATCGTGGGCT CAAAGGTGGA TACTGTGGCT 
AAAGATTGGC ATTGGGCACT TCGGGTGACC CCTGGGCTGG GACTGCTGGC CGTGTTCCTC 
TTAATGCTTG TGGTTCAGGA ACCGAAGCGT GGTGCGATTG AAGCTCACCC CGAGCACACG 
CTGCACAGAA CCAGCTGGCT GGCAGACATG AAAGCACTTT GCAGGAATCC AAGTTTTATT 
CTGTCCACGT TCGGCTTCAC GGCAGTGGCC TTTGTGACGG GATCACTGGC CCTGTGGGCT 
CCTGCCTTTT TATTTAGAGC CGGCGTCTTC ACCGGAGTCA AGCAGCCGTG TTTTAAAGCT 
CCATGTGATG ACTCTGACAG TCTGATTTTT GGCGCCATCA CTGTAGTAAC GGGCATCTTG 
GGTGTAGCGA GCGGTGTTCA GGCCAGTAAA CTGCTGAGGA CCAGAACTCC TCGAGCTGAC 
CCGCTAGTGT GTGCAGCTGG ACTCCTCCTC GCGGCTCCCT TTCTCTATCT CTCCATCATG 
TTTGCTCAGG CGAGCACTGT GGCCACATAT GTCTTTATCT TCCTGGGAGA GACATTCCTT 
TCAATGAATT GGGCCATTGT TGCTGATATC CTACTGTATG TTGTGATCCC GACACGTCGC 
TCCACAGCAG AGGCCTTTCA GATTGTCCTT TCACACTTGC TGGGTGACGC TATTAGTCCG 
TATCTCATAG GAGTGGTTTC AGACTCAATA AAAGAATCAA ATTCGTACAT GTGGGAGTTT 
CGCTCACTTC AGATGTCTCT GCTGCTGTGC TCTTTCGTGG CCGTCGCTGG AGGCGCTTTC 
TTCTTGGCCA CAGCCGTCTT TATTGAGAAA GACCGAGATC TCGCTGAGAA CTATGTGCCC 
TCTGATGACG CACCAATTGT TGTTCCAAGG AGCGGCAGAT CCACTAAAGT ATCAGTGTCG 
AGTGTTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase.
Autophagy13(2)386-403(2017 Feb)
Sasaki T,Lian S,Khan A,Llop JR,Samuelson AV,Chen W,Klionsky DJ,Kishi S

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Aberrant autolysosomal regulation is linked to the induction of embryonic senescence: differential roles of Beclin 1 and p53 in vertebrate Spns1 deficiency.
PLoS genetics10(6)e1004409(2014 Jun)
Sasaki T,Lian S,Qi J,Bayliss PE,Carr CE,Johnson JL,Guha S,Kobler P,Catz SD,Gill M,Jia K,Klionsky DJ,Kishi S

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists223(2)298-305(2002 Mar)
Young RM,Marty S,Nakano Y,Wang H,Yamamoto D,Lin S,Allende ML

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SPNS1 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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