sema3fb cDNA ORF clone, Danio rerio(zebrafish)

The following sema3fb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3fb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa17258 XM_005172909.3
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa17258 XM_021479878.1
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa17259 XM_009306194.2
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa17259 XM_021479877.1
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa145325 XM_009306194.3
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa145326 XM_005172909.4
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa145327 XM_021472495.1
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa145328 XM_021472496.1
Latest version!
Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa17258
    Clone ID Related Accession (Same CDS sequence) XM_021479878.1 , XM_005172909.3
    Accession Version XM_021479878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-3F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC
    AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC
    ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA
    GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA
    GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA
    GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC
    GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC
    TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG
    GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC
    ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT
    CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335553.1
    CDS396..2759
    Translation

    Target ORF information:

    RefSeq Version XM_021479878.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC
    AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC
    ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA
    GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA
    GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA
    GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC
    GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC
    TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG
    GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC
    ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT
    CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa17258
    Clone ID Related Accession (Same CDS sequence) XM_021479878.1 , XM_005172909.3
    Accession Version XM_005172909.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-3F isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC
    AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC
    ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA
    GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA
    GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA
    GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC
    GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC
    TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG
    GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC
    ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT
    CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172966.1
    CDS375..2738
    Translation

    Target ORF information:

    RefSeq Version XM_005172909.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172909.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC
    AATTCCAACA TTAATAAGAA CACACTGGAG ACAGTGCAGT ATGGGGTGGA GGGCAGCAGC
    ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA
    GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA
    GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA
    GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC
    GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC
    TGGACCCCCA GTGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG
    GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC
    ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT
    CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa17259
    Clone ID Related Accession (Same CDS sequence) XM_009306194.2 , XM_021479877.1
    Accession Version XM_009306194.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-3F isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG
    TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG
    GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG
    CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG
    AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC
    TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG
    ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC
    CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC
    CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT
    CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA
    AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304469.1
    CDS375..2750
    Translation

    Target ORF information:

    RefSeq Version XM_009306194.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306194.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG
    TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG
    GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG
    CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG
    AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC
    TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG
    ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC
    CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC
    CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT
    CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA
    AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa17259
    Clone ID Related Accession (Same CDS sequence) XM_009306194.2 , XM_021479877.1
    Accession Version XM_021479877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG
    TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG
    GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG
    CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG
    AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC
    TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG
    ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC
    CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC
    CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT
    CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA
    AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335552.1
    CDS397..2772
    Translation

    Target ORF information:

    RefSeq Version XM_021479877.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCC
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAGACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GTGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CAGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCCCTGGTGG TCAGGACTAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAGAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCATG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAG
    AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG
    TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACAGTGCA GTATGGGGTG
    GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG
    CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG
    AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC
    TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG
    ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC
    CAGTCCAGCG CCTGGACCCC CAGTGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC
    CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT
    CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA
    AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145325
    Clone ID Related Accession (Same CDS sequence) XM_009306194.3
    Accession Version XM_009306194.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-3F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306194.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA
    AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG
    TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACGGTGCA GTATGGGGTG
    GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG
    CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG
    AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC
    TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG
    ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC
    CAGTCCAGCG CCTGGACCCC CAGCGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC
    CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT
    CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA
    AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304469.2
    CDS397..2772
    Translation

    Target ORF information:

    RefSeq Version XM_009306194.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306194.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA
    AGGCTTCCTT TTAGACGCAG CCGTCGACAA GACGTGAAAT ACGGGAACCC CATACGCCAG
    TGCAGAGGAT ACAATTCCAA CATTAATAAG AACACACTGG AGACGGTGCA GTATGGGGTG
    GAGGGCAGCA GCACCTTTCT GGAGTGCCAG GCCAGATCAC CACACGCTGC CATCAAGTGG
    CACCTTCACA GAGACAACAG CGACCGGAGG AGAGAAGTCC GCAGTGATGG CCGTGTGCTG
    AAGACGGATC AAGGTTTGCT TCTGCGCTCT CTCCAGTCCT CAGACTCCGG CATCTACATC
    TGCACGGCCA CAGAGAAGAA CTTCAAGCAC ACGCTGGTCA AACTGCAGCT GCTCGTCCTG
    ACCAATCAGG CCGTCAACAA CATTCTGGTG GACACGGGAC GTCCGGTCGT CTCTCCGCTC
    CAGTCCAGCG CCTGGACCCC CAGCGCCGGG CAATACAAAG ACCTGCTGAC CATCCTCAGC
    CAGCCCGAGA TGGGCCTCAT AAACCAGTAC TGCCAGGACT ATTGGCAGTA TGGAGACCCT
    CTGACCGGGG TCATCAAGGC CAAAGACCTC AAAGAGCTCA AGGAGCAGAA GAAACCACGA
    AATCGCAGAC ATCACGGGGA CGAGGAGGAG ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145326
    Clone ID Related Accession (Same CDS sequence) XM_005172909.4
    Accession Version XM_005172909.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-3F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172909.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA
    AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC
    AATTCCAACA TTAATAAGAA CACACTGGAG ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC
    ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA
    GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA
    GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA
    GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC
    GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC
    TGGACCCCCA GCGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG
    GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC
    ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT
    CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172966.2
    CDS396..2759
    Translation

    Target ORF information:

    RefSeq Version XM_005172909.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172909.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGACGG CCATGCATCT GCAGATGCCC
    CAGACCAGAG GCAGAGCTAG TCGTGCAGCT GAGCCTGAAA CAGTGTTTGA TGAAACAGGA
    CTGCAGGAAT ACGTGTTCCA TTTGGAGCCC GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC
    CCGTATGATC CAAAACTGAA CAGCGTCTCT GCTTTGATTA ATGGAGAACT GTATGCAGGG
    GTGTATATTG ACTTCATGGG AACAGACTCT GCTATTTTCC GCACTTTGGG GAAAAACGCA
    GTCATGCGTA CCGACCAATA CAACTCAAGA TGGCTGAATG ACCCATCATT TGTCCATGTG
    CACCTCATTC CTGACAGCGG TGAGAAAAAC GATGACAAAC TCTACTTTTT CTTTCGTGAA
    AAGTCTTCTG AAATGGGCCA GAGCCCGAAA TCACAATCCC GCATCGGCCG CATCTGCCTG
    AACGATGACG GCGGTCACTG CTGCTTGGTC AACAAGTGGA GCACCTTCCT GAAAGCCCGT
    CTCATCTGCT CGGTCCCAGG AGCTGACGGG ATTGAAACGC ACTTCGATGA GCTCAGAGAT
    GTGTACATAC AGCCCACGCA GGACACTAAA AACCCAGTCA TCTACGGGGT CTTCTCTGTG
    TCCGGCTCTG TATTCAAGGG CTCGGCGGTC TGCGTCTACT CCATGGCGGA CATTCGGATG
    GTGTTCAATG GACCTTTTGC CCACAAAGAA GGTCCCAATT ACCAGTGGGT GGCCTACACT
    GGGAAAATCC CCTACCCCCG TCCGGGAACA TGTCCTGGTG GGACTTTCAC CCCGAACATG
    AAGTCCACTA AGGACTACCC AGACGAGGTC ATCAACTTCA TGCGGAATCA TCCCACCATG
    TATAACTCCG TGTATCCAGT GCACAAGCGC CCTCTGGTGG TCAGGACCAA CGTGGACTAT
    GAGTTCACAA CCATCACTGT GGATCAGGTC ACAGCGGCCG ACGGAAACTA TGAGGTGCTC
    TTCCTGGGAA CAGACAAAGG AACAGTGCAG AAGGTGATCG TCCTACCCAG TGATGACCTG
    CAGACAGAGG AGCTTGTGCT GGAGGAAGTG GAGGTCTTTA AGGTCCCTAC TTCTATCACA
    ACAATGAAGA TTTCTTCAAA ACGGCAACAA CTGTATGTGG CCTCTGCGGT GGGAGTGACG
    CATATGGCTC TGCACCGCTG TGATGTCTAC GGCGAGGCCT GTGCTGACTG CTGTTTGGCC
    CGGGACCCCT ATTGTGCCTG GGACGGCAAA TCCTGCTCTA GATACTCAGC CTCGCAGAAA
    AGACGCAGCC GTCGACAAGA CGTGAAATAC GGGAACCCCA TACGCCAGTG CAGAGGATAC
    AATTCCAACA TTAATAAGAA CACACTGGAG ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC
    ACCTTTCTGG AGTGCCAGGC CAGATCACCA CACGCTGCCA TCAAGTGGCA CCTTCACAGA
    GACAACAGCG ACCGGAGGAG AGAAGTCCGC AGTGATGGCC GTGTGCTGAA GACGGATCAA
    GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA GACTCCGGCA TCTACATCTG CACGGCCACA
    GAGAAGAACT TCAAGCACAC GCTGGTCAAA CTGCAGCTGC TCGTCCTGAC CAATCAGGCC
    GTCAACAACA TTCTGGTGGA CACGGGACGT CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC
    TGGACCCCCA GCGCCGGGCA ATACAAAGAC CTGCTGACCA TCCTCAGCCA GCCCGAGATG
    GGCCTCATAA ACCAGTACTG CCAGGACTAT TGGCAGTATG GAGACCCTCT GACCGGGGTC
    ATCAAGGCCA AAGACCTCAA AGAGCTCAAG GAGCAGAAGA AACCACGAAA TCGCAGACAT
    CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145327
    Clone ID Related Accession (Same CDS sequence) XM_021472495.1
    Accession Version XM_021472495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-3F isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC
    GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT
    GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT
    GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA
    TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC
    GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA
    TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC
    AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG
    ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA
    AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC
    TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA
    GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA
    TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC
    ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC
    CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC
    ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG
    AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG
    GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA
    CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC
    GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA
    TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGGCTTCCTT TTAGACGCAG CCGTCGACAA
    GACGTGAAAT ACGGGAACCC CATACGCCAG TGCAGAGGAT ACAATTCCAA CATTAATAAG
    AACACACTGG AGACGGTGCA GTATGGGGTG GAGGGCAGCA GCACCTTTCT GGAGTGCCAG
    GCCAGATCAC CACACGCTGC CATCAAGTGG CACCTTCACA GAGACAACAG CGACCGGAGG
    AGAGAAGTCC GCAGTGATGG CCGTGTGCTG AAGACGGATC AAGGTTTGCT TCTGCGCTCT
    CTCCAGTCCT CAGACTCCGG CATCTACATC TGCACGGCCA CAGAGAAGAA CTTCAAGCAC
    ACGCTGGTCA AACTGCAGCT GCTCGTCCTG ACCAATCAGG CCGTCAACAA CATTCTGGTG
    GACACGGGAC GTCCGGTCGT CTCTCCGCTC CAGTCCAGCG CCTGGACCCC CAGCGCCGGG
    CAATACAAAG ACCTGCTGAC CATCCTCAGC CAGCCCGAGA TGGGCCTCAT AAACCAGTAC
    TGCCAGGACT ATTGGCAGTA TGGAGACCCT CTGACCGGGG TCATCAAGGC CAAAGACCTC
    AAAGAGCTCA AGGAGCAGAA GAAACCACGA AATCGCAGAC ATCACGGGGA CGAGGAGGAG
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328170.1
    CDS397..2682
    Translation

    Target ORF information:

    RefSeq Version XM_021472495.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC
    GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT
    GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT
    GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA
    TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC
    GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA
    TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC
    AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG
    ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA
    AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC
    TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA
    GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA
    TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC
    ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC
    CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC
    ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG
    AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG
    GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA
    CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC
    GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA
    TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGGCTTCCTT TTAGACGCAG CCGTCGACAA
    GACGTGAAAT ACGGGAACCC CATACGCCAG TGCAGAGGAT ACAATTCCAA CATTAATAAG
    AACACACTGG AGACGGTGCA GTATGGGGTG GAGGGCAGCA GCACCTTTCT GGAGTGCCAG
    GCCAGATCAC CACACGCTGC CATCAAGTGG CACCTTCACA GAGACAACAG CGACCGGAGG
    AGAGAAGTCC GCAGTGATGG CCGTGTGCTG AAGACGGATC AAGGTTTGCT TCTGCGCTCT
    CTCCAGTCCT CAGACTCCGG CATCTACATC TGCACGGCCA CAGAGAAGAA CTTCAAGCAC
    ACGCTGGTCA AACTGCAGCT GCTCGTCCTG ACCAATCAGG CCGTCAACAA CATTCTGGTG
    GACACGGGAC GTCCGGTCGT CTCTCCGCTC CAGTCCAGCG CCTGGACCCC CAGCGCCGGG
    CAATACAAAG ACCTGCTGAC CATCCTCAGC CAGCCCGAGA TGGGCCTCAT AAACCAGTAC
    TGCCAGGACT ATTGGCAGTA TGGAGACCCT CTGACCGGGG TCATCAAGGC CAAAGACCTC
    AAAGAGCTCA AGGAGCAGAA GAAACCACGA AATCGCAGAC ATCACGGGGA CGAGGAGGAG
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145328
    Clone ID Related Accession (Same CDS sequence) XM_021472496.1
    Accession Version XM_021472496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-3F isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394824.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC
    GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT
    GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT
    GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA
    TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC
    GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA
    TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC
    AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG
    ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA
    AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC
    TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA
    GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA
    TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC
    ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC
    CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC
    ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG
    AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG
    GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA
    CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC
    GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA
    TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGACGCAGCC GTCGACAAGA CGTGAAATAC
    GGGAACCCCA TACGCCAGTG CAGAGGATAC AATTCCAACA TTAATAAGAA CACACTGGAG
    ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC ACCTTTCTGG AGTGCCAGGC CAGATCACCA
    CACGCTGCCA TCAAGTGGCA CCTTCACAGA GACAACAGCG ACCGGAGGAG AGAAGTCCGC
    AGTGATGGCC GTGTGCTGAA GACGGATCAA GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA
    GACTCCGGCA TCTACATCTG CACGGCCACA GAGAAGAACT TCAAGCACAC GCTGGTCAAA
    CTGCAGCTGC TCGTCCTGAC CAATCAGGCC GTCAACAACA TTCTGGTGGA CACGGGACGT
    CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC TGGACCCCCA GCGCCGGGCA ATACAAAGAC
    CTGCTGACCA TCCTCAGCCA GCCCGAGATG GGCCTCATAA ACCAGTACTG CCAGGACTAT
    TGGCAGTATG GAGACCCTCT GACCGGGGTC ATCAAGGCCA AAGACCTCAA AGAGCTCAAG
    GAGCAGAAGA AACCACGAAA TCGCAGACAT CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328171.1
    CDS397..2670
    Translation

    Target ORF information:

    RefSeq Version XM_021472496.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb (sema3fb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTCTTGG ACAGTCTATG GCCAGTCCAG GTTCTCCTGG TTCTGTCTGT CCTAACGTTG 
    GTCTTGGGCA ATGAACCTCA AAATGCCCCG CGGGTCTTCT TGTCCTTCAA AGATCTGAGG
    TCTACAGGTA CAGCCCATTA CTTCTCTTTC CTGCTCAACA CATCTGACTA TCGCATCTTG
    CGCATGGATG AGGATCACGA CCGCATGTAT GTGGGCAGCA AAGATTACAT CCTGTCTCTG
    GATCTCCACG ACATCAACCG AGAGCCACTC ATAATACACT GGCCTGTTTC CCCGCAGAGG
    AAGACTGAGT GTGTTTTAAG TGGCAAAGAC ACAAACGGCG AGTGTGGTAA TTTCATCCGT
    CTGATCGAGC CATGGAACAG GACGCACTTA TATATTTGTG GGACGGGCGC CTACAACCCT
    GTGTGCACTT ATGTGAACAG AGGACGCAAG CCTATGGAAT ACGTGTTCCA TTTGGAGCCC
    GGCAAGGAAG ATTCTGGGAA GGGAAAGTGC CCGTATGATC CAAAACTGAA CAGCGTCTCT
    GCTTTGATTA ATGGAGAACT GTATGCAGGG GTGTATATTG ACTTCATGGG AACAGACTCT
    GCTATTTTCC GCACTTTGGG GAAAAACGCA GTCATGCGTA CCGACCAATA CAACTCAAGA
    TGGCTGAATG ACCCATCATT TGTCCATGTG CACCTCATTC CTGACAGCGG TGAGAAAAAC
    GATGACAAAC TCTACTTTTT CTTTCGTGAA AAGTCTTCTG AAATGGGCCA GAGCCCGAAA
    TCACAATCCC GCATCGGCCG CATCTGCCTG AACGATGACG GCGGTCACTG CTGCTTGGTC
    AACAAGTGGA GCACCTTCCT GAAAGCCCGT CTCATCTGCT CGGTCCCAGG AGCTGACGGG
    ATTGAAACGC ACTTCGATGA GCTCAGAGAT GTGTACATAC AGCCCACGCA GGACACTAAA
    AACCCAGTCA TCTACGGGGT CTTCTCTGTG TCCGGCTCTG TATTCAAGGG CTCGGCGGTC
    TGCGTCTACT CCATGGCGGA CATTCGGATG GTGTTCAATG GACCTTTTGC CCACAAAGAA
    GGTCCCAATT ACCAGTGGGT GGCCTACACT GGGAAAATCC CCTACCCCCG TCCGGGAACA
    TGTCCTGGTG GGACTTTCAC CCCGAACATG AAGTCCACTA AGGACTACCC AGACGAGGTC
    ATCAACTTCA TGCGGAATCA TCCCACCATG TATAACTCCG TGTATCCAGT GCACAAGCGC
    CCTCTGGTGG TCAGGACCAA CGTGGACTAT GAGTTCACAA CCATCACTGT GGATCAGGTC
    ACAGCGGCCG ACGGAAACTA TGAGGTGCTC TTCCTGGGAA CAGACAAAGG AACAGTGCAG
    AAGGTGATCG TCCTACCCAG TGATGACCTG CAGACAGAGG AGCTTGTGCT GGAGGAAGTG
    GAGGTCTTTA AGGTCCCTAC TTCTATCACA ACAATGAAGA TTTCTTCAAA ACGGCAACAA
    CTGTATGTGG CCTCTGCGGT GGGAGTGACG CATATGGCTC TGCACCGCTG TGATGTCTAC
    GGCGAGGCCT GTGCTGACTG CTGTTTGGCC CGGGACCCCT ATTGTGCCTG GGACGGCAAA
    TCCTGCTCTA GATACTCAGC CTCGCAGAAA AGACGCAGCC GTCGACAAGA CGTGAAATAC
    GGGAACCCCA TACGCCAGTG CAGAGGATAC AATTCCAACA TTAATAAGAA CACACTGGAG
    ACGGTGCAGT ATGGGGTGGA GGGCAGCAGC ACCTTTCTGG AGTGCCAGGC CAGATCACCA
    CACGCTGCCA TCAAGTGGCA CCTTCACAGA GACAACAGCG ACCGGAGGAG AGAAGTCCGC
    AGTGATGGCC GTGTGCTGAA GACGGATCAA GGTTTGCTTC TGCGCTCTCT CCAGTCCTCA
    GACTCCGGCA TCTACATCTG CACGGCCACA GAGAAGAACT TCAAGCACAC GCTGGTCAAA
    CTGCAGCTGC TCGTCCTGAC CAATCAGGCC GTCAACAACA TTCTGGTGGA CACGGGACGT
    CCGGTCGTCT CTCCGCTCCA GTCCAGCGCC TGGACCCCCA GCGCCGGGCA ATACAAAGAC
    CTGCTGACCA TCCTCAGCCA GCCCGAGATG GGCCTCATAA ACCAGTACTG CCAGGACTAT
    TGGCAGTATG GAGACCCTCT GACCGGGGTC ATCAAGGCCA AAGACCTCAA AGAGCTCAAG
    GAGCAGAAGA AACCACGAAA TCGCAGACAT CACGGGGACG AGGAGGAGAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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