robo3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	robo3
Entrez Gene ID	33314
Full Name	roundabout 3
Synonyms	CG14345,CG5423,CT17158,Dmel\CG5423,ROBO3,Robo 3,Robo-3,Robo3,dRobo-3
General protein information	Preferred Names roundabout 3 Names CG5423 gene product from transcript CG5423-RB CG5423-PA CG5423-PB robo3-PA robo3-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21F3-21F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272937.1	NP_001259866.1	roundabout 3, isoform B
NM_134748.4	NP_608592.2	roundabout 3, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010242	XP_554155.3
Danio rerio (zebrafish)	robo1	NP_571556.1
Drosophila melanogaster (fruit fly)	robo3	NP_001259866.1
Homo sapiens (human)	ROBO1	NP_002932.1
Pan troglodytes (chimpanzee)	ROBO1	XP_516592.3
Gallus gallus (chicken)	ROBO1	XP_004938403.1
Caenorhabditis elegans (roundworm)	sax-3	NP_001024990.1
Xenopus tropicalis (tropical clawed frog)	robo1	NP_001096171.1
	ROBO1	XP_002802665.1
Canis lupus familiaris (dog)	ROBO1	XP_544814.4
Bos taurus (cattle)	ROBO1	NP_001179817.2
Mus musculus (house mouse)	Robo1	NP_062286.2
Rattus norvegicus (Norway rat)	Robo1	NP_071524.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the role of Slit and Robo receptors in wiring Drosophila higher-order brain circuits and identified differences in the cellular and molecular mechanisms of Robo/Slit function, was investigated.
Title: Regulation of Drosophila Brain Wiring by Neuropil Interactions via a Slit-Robo-RPTP Signaling Complex.

Data show that Robo and Robo2 promote midline repulsion, while Robo2 and Robo3 specify the position of longitudinal axon pathways.
Title: Slit/Robo-mediated axon guidance in Tribolium and Drosophila: divergent genetic programs build insect nervous systems.

The transient cell-specific expression of Robo3 plays a crucial role in establishing neural circuits in the Drosophila visual system by selectively regulating pathway choice for posterior-most R8 growth cones.
Title: Robo-3--mediated repulsive interactions guide R8 axons during Drosophila visual system development.

Slit and Robo-family proteins act to maintain the distinct cellular composition of the developing lamina and the lobula cortex
Title: Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins.

axon guidance molecules roundabout2 and roundabout3 (robo2/3) are necessary for serotonergic neuron differentiation
Title: robo2 and robo3 interact with eagle to regulate serotonergic neuron differentiation.

The following robo3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13448	NM_001272937.1 Latest version!	Drosophila melanogaster roundabout 3 (robo3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa13448	NM_134748.4 Latest version!	Drosophila melanogaster roundabout 3 (robo3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robo3 ( NM_001272937.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259866.1 Drosophila melanogaster roundabout 3 (robo3), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13448
Clone ID Related Accession (Same CDS sequence)	NM_001272937.1 , NM_134748.4
Accession Version	NM_001272937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roundabout 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC 
ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG 
GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT 
TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG 
AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC 
GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC 
GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG 
AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC 
CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG 
GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT 
CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT 
GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT 
CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA 
GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG 
ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC 
GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC 
ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC 
CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG 
GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG 
CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT 
CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT 
CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT 
ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC 
ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG 
TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT 
GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA 
ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC 
AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC 
GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC 
TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG 
GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG 
CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA 
CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA 
CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC 
TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC 
AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG 
GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG 
TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC 
GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG 
GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG 
GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT 
GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC 
GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT 
GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG 
CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG 
CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG 
AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC 
GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG 
GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG 
CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA 
GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG 
GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA 
AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG 
ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG 
CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG 
AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG 
GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC 
GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT 
GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA 
AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT 
CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC 
TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC 
TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC 
TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC 
AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA 
GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC 
AATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259866.1
CDS	475..4503
Translation	MDLRFVFIVFLLKWTHAQGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKILPGSHRITLPAGGLFFLKVVNSRRETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGIPEPTVTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLKVHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLERVTIADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFWTIKNNSTLIFPGAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLDSQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQPSSKLNITTSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIELPTNPNIKFYRAPEQTKCPSAPGQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAAYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEANASDSTTARLSWDIDSGQYIEGFYLYARELHSSEYKMVTLLNKGQGLSSCTVPGLAKASTYEFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQFNRTSVFLKWLPPQPNRTRNGILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRIDARTQSLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGALVLFKRYQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVWRQAAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLPDYERLSPLNMPDYAEVACSTFKSPTHGAPPMGGGGGGQSASLYDSCGAYATTNVVANVKLYQNRYATKPNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQQQPNLMTTSTASTAILTNSPAKVKKINITENKMDQLEGKSERTNPFNQQQQLLLASNALKQGLGAYANTTLAAQMASGGGAGTLRRQRQPKTLYKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPPSEGGDFSGLGLCNSTNQLLNDWASSASIAAPGDYHFGSKQPSKQHLYVKAKDGTWSAVSSDAYQSFKHQQQHHPFLAGSGDNTKSLASVNSLAGDSKFLSSFGSSANV

Target ORF information:

RefSeq Version	NM_001272937.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roundabout 3 (robo3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272937.1

ORF Insert Sequence:

ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC 
ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG 
GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT 
TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG 
AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC 
GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC 
GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG 
AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC 
CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG 
GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT 
CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT 
GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT 
CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA 
GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG 
ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC 
GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC 
ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC 
CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG 
GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG 
CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT 
CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT 
CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT 
ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC 
ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG 
TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT 
GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA 
ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC 
AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC 
GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC 
TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG 
GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG 
CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA 
CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA 
CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC 
TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC 
AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG 
GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG 
TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC 
GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG 
GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG 
GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT 
GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC 
GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT 
GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG 
CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG 
CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG 
AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC 
GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG 
GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG 
CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA 
GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG 
GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA 
AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG 
ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG 
CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG 
AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG 
GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC 
GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT 
GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA 
AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT 
CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC 
TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC 
TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC 
TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC 
AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA 
GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC 
AATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( NM_134748.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608592.2 Drosophila melanogaster roundabout 3 (robo3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13448
Clone ID Related Accession (Same CDS sequence)	NM_001272937.1 , NM_134748.4
Accession Version	NM_134748.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roundabout 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134748.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC 
ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG 
GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT 
TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG 
AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC 
GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC 
GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG 
AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC 
CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG 
GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT 
CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT 
GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT 
CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA 
GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG 
ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC 
GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC 
ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC 
CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG 
GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG 
CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT 
CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT 
CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT 
ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC 
ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG 
TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT 
GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA 
ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC 
AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC 
GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC 
TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG 
GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG 
CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA 
CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA 
CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC 
TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC 
AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG 
GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG 
TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC 
GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG 
GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG 
GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT 
GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC 
GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT 
GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG 
CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG 
CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG 
AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC 
GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG 
GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG 
CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA 
GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG 
GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA 
AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG 
ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG 
CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG 
AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG 
GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC 
GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT 
GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA 
AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT 
CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC 
TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC 
TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC 
TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC 
AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA 
GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC 
AATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608592.2
CDS	475..4503
Translation	MDLRFVFIVFLLKWTHAQGSHPPRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKILPGSHRITLPAGGLFFLKVVNSRRETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGIPEPTVTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLKVHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLERVTIADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFWTIKNNSTLIFPGAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLDSQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQPSSKLNITTSGDLIISDLDRQQDQGLYTCVASSRAGKSTWSGFLRIELPTNPNIKFYRAPEQTKCPSAPGQPKILNATASALTIVWPTSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAAYMFIVRAENSLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEANASDSTTARLSWDIDSGQYIEGFYLYARELHSSEYKMVTLLNKGQGLSSCTVPGLAKASTYEFFLVPFYKSIVGKPSNSRRMRTLEDVPEAPPYGMEAIQFNRTSVFLKWLPPQPNRTRNGILTSYNVVVKGLDVHNTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRIDARTQSLDTGYTRYPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGALVLFKRYQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSGVWRQAAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLPDYERLSPLNMPDYAEVACSTFKSPTHGAPPMGGGGGGQSASLYDSCGAYATTNVVANVKLYQNRYATKPNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQQQPNLMTTSTASTAILTNSPAKVKKINITENKMDQLEGKSERTNPFNQQQQLLLASNALKQGLGAYANTTLAAQMASGGGAGTLRRQRQPKTLYKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPPSEGGDFSGLGLCNSTNQLLNDWASSASIAAPGDYHFGSKQPSKQHLYVKAKDGTWSAVSSDAYQSFKHQQQHHPFLAGSGDNTKSLASVNSLAGDSKFLSSFGSSANV

Target ORF information:

RefSeq Version	NM_134748.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roundabout 3 (robo3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134748.4

ORF Insert Sequence:

ATGGATTTAC GTTTCGTATT CATCGTTTTC CTGCTCAAGT GGACCCATGC ACAAGGATCC 
CATCCGCCGC GGATAGTGGA GCATCCCATA GATACGACAG TGCCGCGACA CGAGCCAGCC 
ACGCTTAATT GCAAAGCCGA GGGCAGCCCC ACGCCCACCA TCCAGTGGTA CAAGGATGGG 
GTGCCCCTGA AGATCCTGCC GGGCTCCCAC CGCATCACCC TGCCAGCCGG CGGATTGTTT 
TTCCTCAAGG TTGTCAATTC ACGTCGCGAA ACAGATGCGG GCATCTACTG GTGCGAGGCG 
AAGAACGAAC TGGGCGTGGC AAGGAGTCGG AACGCCACGT TGCAAGTGGC GGTTCTCCGC 
GATGAGTTCC GCCTGGAGCC GCAGAACACA CGGATCGCCC AGGGCGATAC CGCCCTCCTC 
GAGTGCGCCG CCCCTCGGGG AATCCCCGAG CCCACAGTCA CCTGGAAGAA GGGCGGCCAG 
AAATTGGATT TGGAGGGCTC GAAGCGAGTA CGCATCGTTG ACGGCGGCAA TTTGGCCATC 
CAGGATGCCC GACAGACTGA CGAGGGTCAG TACCAGTGCA TAGCCAAGAA TCCTGTCGGG 
GTGCGCGAGT CCTCGTTGGC TACACTCAAA GTGCACGTCA AGCCGTACAT CATCCGGGGT 
CCGCACGATC AAACGGTTTT GGAGGGCGCC TCGGTGACCT TTCCCTGTCG AGTGGGTGGT 
GACCCCATGC CGGACGTACT CTGGCTGCGA ACCGCCTCGG GTGGCAACAT GCCATTGGAT 
CGCGTGAGTG TCCTGGAGGA TCGGAGTCTG CGACTGGAGA GGGTCACCAT TGCGGATGAA 
GGCGAGTACA GTTGCGAGGC GGACAATGTT GTGGGCGCCA TCACCGCGAT GGGAACTTTG 
ACTGTTTACG CCCCCCCGAA ATTCATCCAA CGTCCTGCGA GCAAATCCGT GGAACTCGGC 
GCCGACACAT CGTTCGAGTG TCGAGCTATA GGAAACCCAA AGCCCACCAT CTTCTGGACC 
ATCAAGAACA ACAGCACGCT GATATTTCCC GGAGCCCCGC CCTTGGATCG CTTTCATAGC 
CTCAACACTG AGGAAGGTCA CTCGATACTC ACGTTGACCA GGTTCCAGAG AACGGACAAG 
GATCTGGTCA TCCTGTGCAA CGCCATGAAC GAGGTGGCCA GCATTACCTC AAGGGTGCAG 
CTAAGCCTGG ACTCTCAAGA GGATCGACCA CCGCCGATCA TAATCTCCGG TCCGGTTAAT 
CAAACCCTAC CCATCAAGTC CCTGGCCACA CTTCAGTGCA AGGCCATCGG TTTGCCCAGT 
CCCACTATTT CCTGGTATCG CGATGGAATA CCAGTGCAAC CCAGTTCCAA GCTGAACATT 
ACCACATCCG GGGACTTGAT AATATCGGAC CTCGATCGAC AACAGGATCA AGGCCTATAC 
ACCTGCGTGG CCAGCTCGCG GGCTGGAAAG TCCACTTGGA GTGGTTTCCT CCGGATAGAG 
TTGCCAACCA ATCCGAATAT TAAGTTCTAC AGAGCCCCGG AGCAGACTAA GTGTCCCAGT 
GCTCCAGGGC AACCGAAAAT TCTGAACGCC ACTGCCTCGG CACTGACCAT TGTTTGGCCA 
ACCAGTGACA AGGCGGGAGC ATCGTCATTC CTAGGGTACA GTGTGGAAAT GTACTGCACC 
AATCAGAGCA GGACTTGGAT ACCCATTGCA TCGCGTTTGA GTGAGCCGAT TTTCACGGTC 
GAGAGTTTGA CGCAGGGAGC GGCTTACATG TTCATTGTTC GAGCGGAAAA CTCACTGGGC 
TTCTCGCCAC CCTCCCCCAT ATCGGAACCC ATTACGGCGG GAAAACTTGT GGGCGTGCGG 
GATGGTAGTG AAAGCACCGG GACCTCACAG CTCCTCCTCA GCGATGTGGA GACCTTGCTG 
CAGGCCAACG ATGTTGTGGA GCTACTGGAG GCCAATGCCA GTGATTCGAC GACCGCTCGA 
CTGTCTTGGG ACATAGATAG TGGTCAGTAC ATCGAGGGCT TCTATCTGTA CGCCCGGGAA 
CTGCACTCCT CCGAGTACAA AATGGTTACA CTGCTCAACA AGGGTCAGGG GCTGAGCTCC 
TGTACGGTGC CTGGACTGGC GAAGGCCTCC ACATACGAGT TTTTTCTTGT GCCATTTTAC 
AAGAGCATCG TAGGCAAGCC CTCGAACTCG CGACGCATGA GAACTTTGGA AGATGTTCCG 
GAGGCACCAC CTTATGGCAT GGAAGCCATT CAATTCAATC GCACCTCGGT CTTCTTGAAG 
TGGTTACCTC CACAACCAAA TCGGACTCGC AACGGCATCC TCACCAGCTA CAATGTGGTC 
GTCAAAGGCC TGGACGTGCA CAATACGACG CGGATATTCA AAAATATGAC CATAGACGCG 
GCCGCGCCCA CCCTGCTCCT GGCCAACCTC ACCACAGGAG TCACCTACTA TATCGCAGTG 
GCGGCTGCCA CGCGGGTGGG AGTCGGACCC TTCAGCAAGC CTGCCGTGCT CCGCATCGAT 
GCACGCACCC AATCCCTGGA CACTGGCTAC ACGAGATACC CCATCAGTCG TGATATTGCC 
GATGACTTTT TAACGCAGAC CTGGTTTATC GTCCTGCTGG GCTCCATCAT TGCCATAATT 
GTGTTTCTAT TGGGCGCATT GGTTCTATTC AAGCGCTACC AATTTATCAA GCAGACCTCG 
CTGGGCAGTT TACATGGCAA TCACGCAATC GGAACCGTGC GAAAGTTTCC AACATTGCCG 
CTAAATGGAG GCGGAGCCGT CGGTGCCGGT GGCTCAAATT CGACAGCCGC AGCTGGGCAG 
AGTGGCCATG GCCTGTGGAT CGATCCCACC AGCGGCGTTT GGCGACAGGC AGCGGGTGGC 
GGCAATCCTG GCGGAGTGGG CTCGTGTACG ACAAAGGAGC AACTTCCGGG ATACGCACAG 
GCCACCGCCC AACAAGGACA ACAGCCCACG CTGCTACCCG ATTACGAGAG ACTATCACCG 
CTGAATATGC CCGATTACGC GGAAGTCGCC TGCTCGACAT TCAAAAGCCC CACACACGGA 
GCACCGCCGA TGGGTGGTGG TGGTGGTGGC CAGTCGGCCT CCCTGTACGA CAGCTGTGGG 
GCCTACGCTA CCACCAATGT GGTGGCCAAT GTAAAGCTCT ACCAGAACCG CTATGCGACA 
AAGCCGAACC AGAGCAGCGG CAGCAATAAC AACAATCACC AGGGCAATGA CTACCAGTCG 
ACCGGGATGT ACTCCGCTCC ACCGAGCGCC CACTACGGAT GCTTGGAGCC AAAGCAGCAG 
CAGCAGCAGC AGCAGCCCAA TCTGATGACC ACCTCCACGG CCAGCACGGC CATCTTGACG 
AACTCGCCGG CCAAGGTGAA GAAGATCAAC ATAACCGAGA ACAAAATGGA TCAGCTGGAG 
GGCAAGTCGG AGCGGACGAA CCCGTTCAAC CAGCAGCAGC AGCTCCTCCT GGCCAGCAAC 
GCCCTGAAGC AGGGACTGGG CGCCTATGCC AACACCACCT TGGCGGCTCA GATGGCCAGT 
GGAGGTGGAG CGGGCACCTT GAGGCGCCAG CGTCAGCCGA AGACGCTTTA CAAAAGCGAA 
AACAATATAC TGGGCAAGTC TGGTCTGCGG CAGAACACCA TGAATGCGAG TGTAGGTGCT 
CCCAGCAACG GGCAGATGGA CTTCCTGACC GGCGGTCCTC CGTCGGAGGG CGGAGACTTC 
TCCGGACTGG GCCTCTGCAA CTCCACCAAC CAGCTGCTTA ACGACTGGGC ATCCAGTGCC 
TCGATCGCAG CTCCAGGGGA TTATCATTTC GGCAGCAAGC AGCCCAGCAA GCAGCACCTC 
TACGTGAAGG CCAAGGATGG CACCTGGTCG GCCGTCAGTT CGGATGCCTA TCAGTCCTTC 
AAGCATCAGC AGCAGCACCA TCCCTTCCTG GCAGGATCAG GTGACAACAC CAAGTCCCTA 
GCTAGTGTAA ACAGCTTAGC TGGCGATAGC AAATTCCTGA GTAGTTTTGG CTCTAGTGCC 
AATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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robo3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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