Duox cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Gene Symbol	Duox
Entrez Gene ID	33477
Full Name	Dual oxidase
Synonyms	CG3131,Cy,DUOX,Dmel\CG3131,dDuox,dduox,duox,l(2)23Bb
General protein information	Preferred Names Dual oxidase Names CG3131 gene product from transcript CG3131-RC CG3131-PB CG3131-PC Duox-PB Duox-PC NADPH dual oxidase curly dual oxidase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23B2-23B3\|2-6.1 cM

mRNA and Protein(s)

mRNA Protein Name

NM_001273039.1 NP_001259968.1 dual oxidase, isoform C

NM_134871.3 NP_608715.2 dual oxidase, isoform B
Pathways from BioSystems

mRNA	Protein	Name
NM_001273039.1	NP_001259968.1	dual oxidase, isoform C
NM_134871.3	NP_608715.2	dual oxidase, isoform B

Homologies

	LOC713906	XP_001103398.2
Canis lupus familiaris (dog)	DUOX2	XP_005638424.1
Rattus norvegicus (Norway rat)	Duox2	NP_077055.1
Bos taurus (cattle)	DUOX2	XP_002690988.2
Homo sapiens (human)	DUOX2	NP_054799.4
Drosophila melanogaster (fruit fly)	Duox	NP_001259968.1
Anopheles gambiae (African malaria mosquito)	DUOX	XP_319115.4
Caenorhabditis elegans (roundworm)	bli-3	NP_490686.3
Pan troglodytes (chimpanzee)	DUOX2	XP_510367.3
Arabidopsis thaliana (thale cress)	RBOHB	NP_973799.1
Mus musculus (house mouse)	Duox2	NP_808278.2
Caenorhabditis elegans (roundworm)	duox-2	NP_490684.1
Xenopus tropicalis (tropical clawed frog)	duox2	XP_002937935.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results propose that the uracil/Duox pathway promotes bacteria expulsion from the gut through the HOCl-sensitive receptor, TRPA1(A)10b, thereby minimizing the chances that bacteria adapt to survive host defense systems.
Title: TrpA1 Regulates Defecation of Food-Borne Pathogens under the Control of the Duox Pathway.

wound-induced calcium flash activates DUOX
Title: Calcium flashes orchestrate the wound inflammatory response through DUOX activation and hydrogen peroxide release.

One gene required to activate epidermal wound reporters encodes Dual oxidase, an enzyme that produces hydrogen peroxide.
Title: Duox, Flotillin-2, and Src42A are required to activate or delimit the spread of the transcriptional response to epidermal wounds in Drosophila.

dDuox plays important roles in the stabilization of the cuticle structure of the wings.
Title: Essential role of Duox in stabilization of Drosophila wing.

the Galphaq-PLCbeta-Ca(2+)-DUOX-ROS signaling pathway acts as a bona fide first line of defense that enables gut epithelia to dynamically control yeast during the Drosophila life cycle
Title: Regulation of DUOX by the Galphaq-phospholipase Cbeta-Ca2+ pathway in Drosophila gut immunity.

The following Duox gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Duox cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13550	NM_001273039.1 Latest version!	Drosophila melanogaster dual oxidase, transcript variant C (Duox), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa13550	NM_134871.3 Latest version!	Drosophila melanogaster dual oxidase, transcript variant B (Duox), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Duox ( NM_001273039.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259968.1 Drosophila melanogaster dual oxidase, transcript variant C (Duox), mRNA.

Price & Availability↑

CloneID	OFa13550
Clone ID Related Accession (Same CDS sequence)	NM_001273039.1 , NM_134871.3
Accession Version	NM_001273039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dual oxidase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTTC CAAGTGCGCC CCATCAGAGG GCAGAAAGCA AAAATCGAGT GCCGCGTCCG 
GGCCAAAAAA ACCGCAAGCT CCCCAAGCTC CGGCTCCACT GGCCGGGGGC AACCTACGGA 
GGAGCCCTGC TGCTTCTACT GATTAGTTAC GGTCTGGAGC TGGGCTCCGT GCATTGTTAT 
GAGAAAATGT ATAGTCAGAC CGAGAAACAG CGCTACGATG GCTGGTACAA TAACCTGGCC 
CATCCCGACT GGGGATCCGT GGATAGCCAC CTGGTGCGCA AGGCGCCGCC CTCCTATTCG 
GATGGGGTTT ACGCAATGGC AGGTGCCAAC CGGCCCTCGA CCCGCAGGCT CAGCCGGCTG 
TTTATGCGCG GAAAGGACGG CCTGGGGTCA AAGTTCAACC GGACTGCACT ACTGGCCTTC 
TTCGGCCAGC TGGTGGCCAA CGAGATAGTG ATGGCCTCGG AGTCCGGCTG CCCCATCGAG 
ATGCACCGCA TCGAGATCGA GAAGTGTGAC GAGATGTACG ACCGGGAGTG CCGTGGCGAC 
AAGTACATAC CATTCCATCG GGCGGCCTAC GATCGCGATA CCGGGCAGAG CCCGAATGCG 
CCGAGGGAGC AGATAAATCA AATGACTGCT TGGATTGATG GCAGTTTCAT TTACAGCACC 
TCCGAGGCCT GGCTCAACGC CATGCGCTCC TTCCACAATG GCACCCTGCT CACCGAGAAG 
GATGGGAAGC TTCCGGTGCG GAACACCATG CGGGTGCCGC TCTTCAACAA TCCGGTGCCG 
AGTGTCATGA AGATGCTCAG TCCGGAGCGG CTGTTTCTTC TTGGTGATCC TCGAACCAAT 
CAGAACCCGG CGATCCTCTC CTTCGCCATT CTCTTTCTGC GTTGGCACAA CACCCTGGCC 
CAGCGAATCA AGCGGGTGCA TCCGGATTGG AGCGATGAGG ATATCTATCA GCGAGCACGC 
CACACGGTGA TTGCCAGTCT TCAGAATGTG ATAGTCTACG AGTACCTGCC CGCCTTCCTG 
GGCACCTCGT TGCCACCTTA CGAGGGCTAC AAGCAGGACA TACATCCTGG CATTGGCCAC 
ATCTTCCAGG CGGCCGCCTT TCGGTTCGGC CACACGATGA TTCCGCCGGG AATTTACAGG 
CGAGATGGTC AGTGCAACTT TAAGGAAACG CCCATGGGTT ACCCGGCGGT CAGACTCTGC 
TCCACTTGGT GGGACTCCAG CGGATTTTTT GCCGACACCA GCGTAGAGGA GGTACTAATG 
GGCTTGGCTT CGCAAATATC CGAACGTGAG GATCCGGTTC TCTGTTCAGA TGTGCGGGAC 
AAACTTTTCG GACCGATGGA ATTCACACGC CGGGATCTGG GTGCTCTCAA CATAATGCGG 
GGTCGGGACA ATGGTCTGCC AGATTACAAT ACCGCGAGGG AGTCTTATGG ACTGAAACGG 
CACAAGACCT GGACGGACAT CAATCCTCCG TTGTTCGAAA CGCAGCCGGA ACTCTTGGAC 
ATGCTGAAGG AGGCGTACGA TAACAAGCTG GATGATGTGG ATGTTTATGT GGGTGGTATG 
CTGGAATCCT ACGGTCAACC GGGCGAGTTC TTCACGGCCG TAATCAAGGA GCAGTTCCAG 
CGACTACGAG ATGCCGATAG ATTTTGGTTC GAGAACGAGC GCAACGGCAT ATTTACTCCC 
GAGGAGATCG CTGAGCTACG CAAGATCACC CTGTGGGACA TCATAGTGAA CAGCACGGAT 
GTGAAGGAGG AGGAGATCCA AAAGGATGTG TTCATGTGGC GCACTGGCGA TCCCTGTCCG 
CAGCCAATGC AGCTGAATGC CACCGAACTG GAGCCGTGCA CTTACTTGGA GGGCTACGAC 
TACTTCTCCG GCTCGGAGCT GATGTTTATC TACGTTTGTG TCTTCCTTGG ATTCGTGCCC 
ATATTGTGTG CCGGAGCCGG TTACTGTGTG GTCAAGCTGC AGAACAGCAA GCGCCGTCGG 
CTGAAGATCC GTCAGGAGGC ATTGAGGGCA CCGCAGCACA AGGGATCCGT GGACAAGATG 
CTGGCCAGGG AGTGGCTGCA CGCCAACCAC AAGAGACTGG TCACGGTTAA ATTCGGACCA 
GAGGCGGCCA TCTACACGGT GGATAGGAAG GGCGAGAAGT TGCGCACCTT TAGTTTGAAG 
CACATCGACG TGGTCAGTGT GGAGGAGTCG GCCACGAACC ACATCAAGAA GAAACCTTAC 
ATCCTGCTGC GCGTGCCCAG CGATCATGAT CTGGTGCTCG AGCTGGAATC TTACGGAGCA 
CGCAGGAAGT TTGTAAAGAA GCTGGAGGAT TTCCTACTGC TCCACAAGAA GGAGATGACT 
TTGATGGAGG TCAACAGGGA CATAATGTTG GCGCGGGCGG AGACACGGGA GCGGCGCCAA 
AAGCGACTGG AGTACTTCTT CCGTGAGGCT TACGCCCTGA CCTTTGGCCT GAGACCCGGA 
GAACGACGAA GGCGTTCAGA TGCCTCCAGT GATGGCGAGG TGATGACCGT GATGCGGACC 
AGTCTTTCCA AGGCGGAATT CGCCGCCGCT CTGGGCATGA AGCCCAACGA CATGTTTGTG 
CGCAAGATGT TCAACATTGT GGACAAGGAT CAGGATGGCA GGATTAGCTT CCAGGAGTTC 
CTGGAGACCG TAGTTCTTTT CTCCCGCGGC AAAACGGACG ACAAGCTGCG CATAATCTTT 
GATATGTGTG ATAATGATCG GAATGGCGTC ATCGATAAGG GCGAACTGAG CGAGATGATG 
CGATCGCTGG TGGAGATTGC GAGGACCACG AGCCTGGGTG ATGACCAGGT CACGGAACTG 
ATCGATGGCA TGTTTCAGGA TGTGGGGCTG GAGCACAAGA ACCATCTGAC CTACCAGGAC 
TTCAAACTGA TGATGAAGGA GTACAAGGGT GACTTTGTGG CCATCGGACT GGACTGCAAG 
GGAGCCAAGC AGAACTTCCT GGACACCTCG ACGAACGTGG CCCGGATGAC TTCGTTCAAC 
ATCGAACCGA TGCAGGACAA GCCGCGTCAC TGGTTGCTGG CCAAATGGGA TGCCTACATC 
ACGTTCCTCG AGGAGAACCG GCAGAACATC TTCTATCTGT TCCTCTTCTA CGTGGTCACC 
ATAGTTCTGT TCGTTGAGCG CTTCATCCAC TACTCGTTCA TGGCGGAGCA CACCGATCTG 
CGGCACATTA TGGGCGTAGG CATTGCCATC ACCAGGGGAT CTGCCGCATC GCTGTCCTTC 
TGCTACTCCC TGCTGCTGCT GACCATGTCT CGGAATCTGA TCACCAAGCT GAAGGAGTTC 
CCCATTCAAC AGTACATTCC CCTGGACTCG CACATACAGT TCCACAAGAT TGCCGCCTGC 
ACGGCGCTCT TCTTTTCCGT GCTGCACACG GTGGGCCACA TAGTGAACTT CTATCACGTG 
TCCACCCAAT CGCACGAGAA CCTGCGCTGC CTGACCCGCG AAGTGCACTT CGCCTCCGAC 
TACAAGCCGG ATATCACCTT CTGGCTCTTC CAGACGGTCA CCGGCACCAC AGGCGTCATG 
TTGTTCATCA TCATGTGCAT CATCTTCGTG TTCGCTCATC CTACGATCCG CAAGAAGGCC 
TACAACTTCT TCTGGAACAT GCACACCCTG TACATCGGAT TGTATCTTCT GAGTCTGATC 
CACGGATTGG CTCGACTGAC TGGACCACCA CGCTTCTGGA TGTTCTTCCT GGGCCCCGGA 
ATCGTGTACA CGCTGGATAA GATCGTCTCG CTGCGCACCA AGTACATGGC GCTGGACGTG 
ATCGACACAG ATCTGCTGCC CTCCGACGTG ATCAAGATCA AGTTCTATCG GCCACCGAAT 
CTAAAATACC TTTCTGGCCA ATGGGTGCGG TTGTCCTGTA CCGCCTTCCG GCCGCACGAG 
ATGCACAGCT TCACGCTGAC CTCGGCGCCG CACGAGAACT TCCTCAGCTG CCACATCAAG 
GCCCAAGGTC CGTGGACGTG GAAGCTGCGC AATTACTTCG ATCCCTGCAA CTACAATCCG 
GAGGACCAGC CCAAGATCCG CATCGAGGGA CCATTTGGTG GTGGCAACCA GGACTGGTAC 
AAGTTCGAGG TGGCTGTGAT GGTGGGCGGC GGCATCGGGG TCACTCCATA CGCCTCCATT 
CTCAACGACC TGGTCTTTGG CACCAGTACC AATCGATACT CGGGCGTGGC CTGCAAGAAG 
GTGTACTTCC TGTGGATCTG CCCATCGCAC AAGCACTTTG AGTGGTTCAT CGACGTCCTG 
CGCGATGTGG AGAAGAAGGA TGTGACCAAT GTGCTGGAGA TACACATATT CATCACGCAG 
TTCTTTCACA AGTTCGATCT GCGAACAACA ATGCTGTACA TCTGTGAGAA CCACTTCCAG 
CGACTGTCGA AGACCTCGAT ATTCACGGGT CTCAAGGCGG TGAATCACTT TGGTCGCCCA 
GATATGTCCA GCTTCTTGAA GTTCGTGCAA AAGAAGCACT CATACGTCTC CAAGATAGGA 
GTCTTCTCCT GCGGTCCTCG TCCGCTGACC AAGAGCGTGA TGTCTGCCTG TGATGAAGTG 
AACAAGACGC GCAAGTTGCC CTATTTCATC CACCACTTCG AGAACTTTGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259968.1
CDS	300..4913
Translation	MSVPSAPHQRAESKNRVPRPGQKNRKLPKLRLHWPGATYGGALLLLLISYGLELGSVHCYEKMYSQTEKQRYDGWYNNLAHPDWGSVDSHLVRKAPPSYSDGVYAMAGANRPSTRRLSRLFMRGKDGLGSKFNRTALLAFFGQLVANEIVMASESGCPIEMHRIEIEKCDEMYDRECRGDKYIPFHRAAYDRDTGQSPNAPREQINQMTAWIDGSFIYSTSEAWLNAMRSFHNGTLLTEKDGKLPVRNTMRVPLFNNPVPSVMKMLSPERLFLLGDPRTNQNPAILSFAILFLRWHNTLAQRIKRVHPDWSDEDIYQRARHTVIASLQNVIVYEYLPAFLGTSLPPYEGYKQDIHPGIGHIFQAAAFRFGHTMIPPGIYRRDGQCNFKETPMGYPAVRLCSTWWDSSGFFADTSVEEVLMGLASQISEREDPVLCSDVRDKLFGPMEFTRRDLGALNIMRGRDNGLPDYNTARESYGLKRHKTWTDINPPLFETQPELLDMLKEAYDNKLDDVDVYVGGMLESYGQPGEFFTAVIKEQFQRLRDADRFWFENERNGIFTPEEIAELRKITLWDIIVNSTDVKEEEIQKDVFMWRTGDPCPQPMQLNATELEPCTYLEGYDYFSGSELMFIYVCVFLGFVPILCAGAGYCVVKLQNSKRRRLKIRQEALRAPQHKGSVDKMLAREWLHANHKRLVTVKFGPEAAIYTVDRKGEKLRTFSLKHIDVVSVEESATNHIKKKPYILLRVPSDHDLVLELESYGARRKFVKKLEDFLLLHKKEMTLMEVNRDIMLARAETRERRQKRLEYFFREAYALTFGLRPGERRRRSDASSDGEVMTVMRTSLSKAEFAAALGMKPNDMFVRKMFNIVDKDQDGRISFQEFLETVVLFSRGKTDDKLRIIFDMCDNDRNGVIDKGELSEMMRSLVEIARTTSLGDDQVTELIDGMFQDVGLEHKNHLTYQDFKLMMKEYKGDFVAIGLDCKGAKQNFLDTSTNVARMTSFNIEPMQDKPRHWLLAKWDAYITFLEENRQNIFYLFLFYVVTIVLFVERFIHYSFMAEHTDLRHIMGVGIAITRGSAASLSFCYSLLLLTMSRNLITKLKEFPIQQYIPLDSHIQFHKIAACTALFFSVLHTVGHIVNFYHVSTQSHENLRCLTREVHFASDYKPDITFWLFQTVTGTTGVMLFIIMCIIFVFAHPTIRKKAYNFFWNMHTLYIGLYLLSLIHGLARLTGPPRFWMFFLGPGIVYTLDKIVSLRTKYMALDVIDTDLLPSDVIKIKFYRPPNLKYLSGQWVRLSCTAFRPHEMHSFTLTSAPHENFLSCHIKAQGPWTWKLRNYFDPCNYNPEDQPKIRIEGPFGGGNQDWYKFEVAVMVGGGIGVTPYASILNDLVFGTSTNRYSGVACKKVYFLWICPSHKHFEWFIDVLRDVEKKDVTNVLEIHIFITQFFHKFDLRTTMLYICENHFQRLSKTSIFTGLKAVNHFGRPDMSSFLKFVQKKHSYVSKIGVFSCGPRPLTKSVMSACDEVNKTRKLPYFIHHFENFG

Target ORF information:

RefSeq Version	NM_001273039.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dual oxidase, transcript variant C (Duox), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273039.1

ORF Insert Sequence:

ATGAGTGTTC CAAGTGCGCC CCATCAGAGG GCAGAAAGCA AAAATCGAGT GCCGCGTCCG 
GGCCAAAAAA ACCGCAAGCT CCCCAAGCTC CGGCTCCACT GGCCGGGGGC AACCTACGGA 
GGAGCCCTGC TGCTTCTACT GATTAGTTAC GGTCTGGAGC TGGGCTCCGT GCATTGTTAT 
GAGAAAATGT ATAGTCAGAC CGAGAAACAG CGCTACGATG GCTGGTACAA TAACCTGGCC 
CATCCCGACT GGGGATCCGT GGATAGCCAC CTGGTGCGCA AGGCGCCGCC CTCCTATTCG 
GATGGGGTTT ACGCAATGGC AGGTGCCAAC CGGCCCTCGA CCCGCAGGCT CAGCCGGCTG 
TTTATGCGCG GAAAGGACGG CCTGGGGTCA AAGTTCAACC GGACTGCACT ACTGGCCTTC 
TTCGGCCAGC TGGTGGCCAA CGAGATAGTG ATGGCCTCGG AGTCCGGCTG CCCCATCGAG 
ATGCACCGCA TCGAGATCGA GAAGTGTGAC GAGATGTACG ACCGGGAGTG CCGTGGCGAC 
AAGTACATAC CATTCCATCG GGCGGCCTAC GATCGCGATA CCGGGCAGAG CCCGAATGCG 
CCGAGGGAGC AGATAAATCA AATGACTGCT TGGATTGATG GCAGTTTCAT TTACAGCACC 
TCCGAGGCCT GGCTCAACGC CATGCGCTCC TTCCACAATG GCACCCTGCT CACCGAGAAG 
GATGGGAAGC TTCCGGTGCG GAACACCATG CGGGTGCCGC TCTTCAACAA TCCGGTGCCG 
AGTGTCATGA AGATGCTCAG TCCGGAGCGG CTGTTTCTTC TTGGTGATCC TCGAACCAAT 
CAGAACCCGG CGATCCTCTC CTTCGCCATT CTCTTTCTGC GTTGGCACAA CACCCTGGCC 
CAGCGAATCA AGCGGGTGCA TCCGGATTGG AGCGATGAGG ATATCTATCA GCGAGCACGC 
CACACGGTGA TTGCCAGTCT TCAGAATGTG ATAGTCTACG AGTACCTGCC CGCCTTCCTG 
GGCACCTCGT TGCCACCTTA CGAGGGCTAC AAGCAGGACA TACATCCTGG CATTGGCCAC 
ATCTTCCAGG CGGCCGCCTT TCGGTTCGGC CACACGATGA TTCCGCCGGG AATTTACAGG 
CGAGATGGTC AGTGCAACTT TAAGGAAACG CCCATGGGTT ACCCGGCGGT CAGACTCTGC 
TCCACTTGGT GGGACTCCAG CGGATTTTTT GCCGACACCA GCGTAGAGGA GGTACTAATG 
GGCTTGGCTT CGCAAATATC CGAACGTGAG GATCCGGTTC TCTGTTCAGA TGTGCGGGAC 
AAACTTTTCG GACCGATGGA ATTCACACGC CGGGATCTGG GTGCTCTCAA CATAATGCGG 
GGTCGGGACA ATGGTCTGCC AGATTACAAT ACCGCGAGGG AGTCTTATGG ACTGAAACGG 
CACAAGACCT GGACGGACAT CAATCCTCCG TTGTTCGAAA CGCAGCCGGA ACTCTTGGAC 
ATGCTGAAGG AGGCGTACGA TAACAAGCTG GATGATGTGG ATGTTTATGT GGGTGGTATG 
CTGGAATCCT ACGGTCAACC GGGCGAGTTC TTCACGGCCG TAATCAAGGA GCAGTTCCAG 
CGACTACGAG ATGCCGATAG ATTTTGGTTC GAGAACGAGC GCAACGGCAT ATTTACTCCC 
GAGGAGATCG CTGAGCTACG CAAGATCACC CTGTGGGACA TCATAGTGAA CAGCACGGAT 
GTGAAGGAGG AGGAGATCCA AAAGGATGTG TTCATGTGGC GCACTGGCGA TCCCTGTCCG 
CAGCCAATGC AGCTGAATGC CACCGAACTG GAGCCGTGCA CTTACTTGGA GGGCTACGAC 
TACTTCTCCG GCTCGGAGCT GATGTTTATC TACGTTTGTG TCTTCCTTGG ATTCGTGCCC 
ATATTGTGTG CCGGAGCCGG TTACTGTGTG GTCAAGCTGC AGAACAGCAA GCGCCGTCGG 
CTGAAGATCC GTCAGGAGGC ATTGAGGGCA CCGCAGCACA AGGGATCCGT GGACAAGATG 
CTGGCCAGGG AGTGGCTGCA CGCCAACCAC AAGAGACTGG TCACGGTTAA ATTCGGACCA 
GAGGCGGCCA TCTACACGGT GGATAGGAAG GGCGAGAAGT TGCGCACCTT TAGTTTGAAG 
CACATCGACG TGGTCAGTGT GGAGGAGTCG GCCACGAACC ACATCAAGAA GAAACCTTAC 
ATCCTGCTGC GCGTGCCCAG CGATCATGAT CTGGTGCTCG AGCTGGAATC TTACGGAGCA 
CGCAGGAAGT TTGTAAAGAA GCTGGAGGAT TTCCTACTGC TCCACAAGAA GGAGATGACT 
TTGATGGAGG TCAACAGGGA CATAATGTTG GCGCGGGCGG AGACACGGGA GCGGCGCCAA 
AAGCGACTGG AGTACTTCTT CCGTGAGGCT TACGCCCTGA CCTTTGGCCT GAGACCCGGA 
GAACGACGAA GGCGTTCAGA TGCCTCCAGT GATGGCGAGG TGATGACCGT GATGCGGACC 
AGTCTTTCCA AGGCGGAATT CGCCGCCGCT CTGGGCATGA AGCCCAACGA CATGTTTGTG 
CGCAAGATGT TCAACATTGT GGACAAGGAT CAGGATGGCA GGATTAGCTT CCAGGAGTTC 
CTGGAGACCG TAGTTCTTTT CTCCCGCGGC AAAACGGACG ACAAGCTGCG CATAATCTTT 
GATATGTGTG ATAATGATCG GAATGGCGTC ATCGATAAGG GCGAACTGAG CGAGATGATG 
CGATCGCTGG TGGAGATTGC GAGGACCACG AGCCTGGGTG ATGACCAGGT CACGGAACTG 
ATCGATGGCA TGTTTCAGGA TGTGGGGCTG GAGCACAAGA ACCATCTGAC CTACCAGGAC 
TTCAAACTGA TGATGAAGGA GTACAAGGGT GACTTTGTGG CCATCGGACT GGACTGCAAG 
GGAGCCAAGC AGAACTTCCT GGACACCTCG ACGAACGTGG CCCGGATGAC TTCGTTCAAC 
ATCGAACCGA TGCAGGACAA GCCGCGTCAC TGGTTGCTGG CCAAATGGGA TGCCTACATC 
ACGTTCCTCG AGGAGAACCG GCAGAACATC TTCTATCTGT TCCTCTTCTA CGTGGTCACC 
ATAGTTCTGT TCGTTGAGCG CTTCATCCAC TACTCGTTCA TGGCGGAGCA CACCGATCTG 
CGGCACATTA TGGGCGTAGG CATTGCCATC ACCAGGGGAT CTGCCGCATC GCTGTCCTTC 
TGCTACTCCC TGCTGCTGCT GACCATGTCT CGGAATCTGA TCACCAAGCT GAAGGAGTTC 
CCCATTCAAC AGTACATTCC CCTGGACTCG CACATACAGT TCCACAAGAT TGCCGCCTGC 
ACGGCGCTCT TCTTTTCCGT GCTGCACACG GTGGGCCACA TAGTGAACTT CTATCACGTG 
TCCACCCAAT CGCACGAGAA CCTGCGCTGC CTGACCCGCG AAGTGCACTT CGCCTCCGAC 
TACAAGCCGG ATATCACCTT CTGGCTCTTC CAGACGGTCA CCGGCACCAC AGGCGTCATG 
TTGTTCATCA TCATGTGCAT CATCTTCGTG TTCGCTCATC CTACGATCCG CAAGAAGGCC 
TACAACTTCT TCTGGAACAT GCACACCCTG TACATCGGAT TGTATCTTCT GAGTCTGATC 
CACGGATTGG CTCGACTGAC TGGACCACCA CGCTTCTGGA TGTTCTTCCT GGGCCCCGGA 
ATCGTGTACA CGCTGGATAA GATCGTCTCG CTGCGCACCA AGTACATGGC GCTGGACGTG 
ATCGACACAG ATCTGCTGCC CTCCGACGTG ATCAAGATCA AGTTCTATCG GCCACCGAAT 
CTAAAATACC TTTCTGGCCA ATGGGTGCGG TTGTCCTGTA CCGCCTTCCG GCCGCACGAG 
ATGCACAGCT TCACGCTGAC CTCGGCGCCG CACGAGAACT TCCTCAGCTG CCACATCAAG 
GCCCAAGGTC CGTGGACGTG GAAGCTGCGC AATTACTTCG ATCCCTGCAA CTACAATCCG 
GAGGACCAGC CCAAGATCCG CATCGAGGGA CCATTTGGTG GTGGCAACCA GGACTGGTAC 
AAGTTCGAGG TGGCTGTGAT GGTGGGCGGC GGCATCGGGG TCACTCCATA CGCCTCCATT 
CTCAACGACC TGGTCTTTGG CACCAGTACC AATCGATACT CGGGCGTGGC CTGCAAGAAG 
GTGTACTTCC TGTGGATCTG CCCATCGCAC AAGCACTTTG AGTGGTTCAT CGACGTCCTG 
CGCGATGTGG AGAAGAAGGA TGTGACCAAT GTGCTGGAGA TACACATATT CATCACGCAG 
TTCTTTCACA AGTTCGATCT GCGAACAACA ATGCTGTACA TCTGTGAGAA CCACTTCCAG 
CGACTGTCGA AGACCTCGAT ATTCACGGGT CTCAAGGCGG TGAATCACTT TGGTCGCCCA 
GATATGTCCA GCTTCTTGAA GTTCGTGCAA AAGAAGCACT CATACGTCTC CAAGATAGGA 
GTCTTCTCCT GCGGTCCTCG TCCGCTGACC AAGAGCGTGA TGTCTGCCTG TGATGAAGTG 
AACAAGACGC GCAAGTTGCC CTATTTCATC CACCACTTCG AGAACTTTGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Duox ( NM_134871.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608715.2 Drosophila melanogaster dual oxidase, transcript variant B (Duox), mRNA.

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CloneID	OFa13550
Clone ID Related Accession (Same CDS sequence)	NM_001273039.1 , NM_134871.3
Accession Version	NM_134871.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dual oxidase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134871.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTTC CAAGTGCGCC CCATCAGAGG GCAGAAAGCA AAAATCGAGT GCCGCGTCCG 
GGCCAAAAAA ACCGCAAGCT CCCCAAGCTC CGGCTCCACT GGCCGGGGGC AACCTACGGA 
GGAGCCCTGC TGCTTCTACT GATTAGTTAC GGTCTGGAGC TGGGCTCCGT GCATTGTTAT 
GAGAAAATGT ATAGTCAGAC CGAGAAACAG CGCTACGATG GCTGGTACAA TAACCTGGCC 
CATCCCGACT GGGGATCCGT GGATAGCCAC CTGGTGCGCA AGGCGCCGCC CTCCTATTCG 
GATGGGGTTT ACGCAATGGC AGGTGCCAAC CGGCCCTCGA CCCGCAGGCT CAGCCGGCTG 
TTTATGCGCG GAAAGGACGG CCTGGGGTCA AAGTTCAACC GGACTGCACT ACTGGCCTTC 
TTCGGCCAGC TGGTGGCCAA CGAGATAGTG ATGGCCTCGG AGTCCGGCTG CCCCATCGAG 
ATGCACCGCA TCGAGATCGA GAAGTGTGAC GAGATGTACG ACCGGGAGTG CCGTGGCGAC 
AAGTACATAC CATTCCATCG GGCGGCCTAC GATCGCGATA CCGGGCAGAG CCCGAATGCG 
CCGAGGGAGC AGATAAATCA AATGACTGCT TGGATTGATG GCAGTTTCAT TTACAGCACC 
TCCGAGGCCT GGCTCAACGC CATGCGCTCC TTCCACAATG GCACCCTGCT CACCGAGAAG 
GATGGGAAGC TTCCGGTGCG GAACACCATG CGGGTGCCGC TCTTCAACAA TCCGGTGCCG 
AGTGTCATGA AGATGCTCAG TCCGGAGCGG CTGTTTCTTC TTGGTGATCC TCGAACCAAT 
CAGAACCCGG CGATCCTCTC CTTCGCCATT CTCTTTCTGC GTTGGCACAA CACCCTGGCC 
CAGCGAATCA AGCGGGTGCA TCCGGATTGG AGCGATGAGG ATATCTATCA GCGAGCACGC 
CACACGGTGA TTGCCAGTCT TCAGAATGTG ATAGTCTACG AGTACCTGCC CGCCTTCCTG 
GGCACCTCGT TGCCACCTTA CGAGGGCTAC AAGCAGGACA TACATCCTGG CATTGGCCAC 
ATCTTCCAGG CGGCCGCCTT TCGGTTCGGC CACACGATGA TTCCGCCGGG AATTTACAGG 
CGAGATGGTC AGTGCAACTT TAAGGAAACG CCCATGGGTT ACCCGGCGGT CAGACTCTGC 
TCCACTTGGT GGGACTCCAG CGGATTTTTT GCCGACACCA GCGTAGAGGA GGTACTAATG 
GGCTTGGCTT CGCAAATATC CGAACGTGAG GATCCGGTTC TCTGTTCAGA TGTGCGGGAC 
AAACTTTTCG GACCGATGGA ATTCACACGC CGGGATCTGG GTGCTCTCAA CATAATGCGG 
GGTCGGGACA ATGGTCTGCC AGATTACAAT ACCGCGAGGG AGTCTTATGG ACTGAAACGG 
CACAAGACCT GGACGGACAT CAATCCTCCG TTGTTCGAAA CGCAGCCGGA ACTCTTGGAC 
ATGCTGAAGG AGGCGTACGA TAACAAGCTG GATGATGTGG ATGTTTATGT GGGTGGTATG 
CTGGAATCCT ACGGTCAACC GGGCGAGTTC TTCACGGCCG TAATCAAGGA GCAGTTCCAG 
CGACTACGAG ATGCCGATAG ATTTTGGTTC GAGAACGAGC GCAACGGCAT ATTTACTCCC 
GAGGAGATCG CTGAGCTACG CAAGATCACC CTGTGGGACA TCATAGTGAA CAGCACGGAT 
GTGAAGGAGG AGGAGATCCA AAAGGATGTG TTCATGTGGC GCACTGGCGA TCCCTGTCCG 
CAGCCAATGC AGCTGAATGC CACCGAACTG GAGCCGTGCA CTTACTTGGA GGGCTACGAC 
TACTTCTCCG GCTCGGAGCT GATGTTTATC TACGTTTGTG TCTTCCTTGG ATTCGTGCCC 
ATATTGTGTG CCGGAGCCGG TTACTGTGTG GTCAAGCTGC AGAACAGCAA GCGCCGTCGG 
CTGAAGATCC GTCAGGAGGC ATTGAGGGCA CCGCAGCACA AGGGATCCGT GGACAAGATG 
CTGGCCAGGG AGTGGCTGCA CGCCAACCAC AAGAGACTGG TCACGGTTAA ATTCGGACCA 
GAGGCGGCCA TCTACACGGT GGATAGGAAG GGCGAGAAGT TGCGCACCTT TAGTTTGAAG 
CACATCGACG TGGTCAGTGT GGAGGAGTCG GCCACGAACC ACATCAAGAA GAAACCTTAC 
ATCCTGCTGC GCGTGCCCAG CGATCATGAT CTGGTGCTCG AGCTGGAATC TTACGGAGCA 
CGCAGGAAGT TTGTAAAGAA GCTGGAGGAT TTCCTACTGC TCCACAAGAA GGAGATGACT 
TTGATGGAGG TCAACAGGGA CATAATGTTG GCGCGGGCGG AGACACGGGA GCGGCGCCAA 
AAGCGACTGG AGTACTTCTT CCGTGAGGCT TACGCCCTGA CCTTTGGCCT GAGACCCGGA 
GAACGACGAA GGCGTTCAGA TGCCTCCAGT GATGGCGAGG TGATGACCGT GATGCGGACC 
AGTCTTTCCA AGGCGGAATT CGCCGCCGCT CTGGGCATGA AGCCCAACGA CATGTTTGTG 
CGCAAGATGT TCAACATTGT GGACAAGGAT CAGGATGGCA GGATTAGCTT CCAGGAGTTC 
CTGGAGACCG TAGTTCTTTT CTCCCGCGGC AAAACGGACG ACAAGCTGCG CATAATCTTT 
GATATGTGTG ATAATGATCG GAATGGCGTC ATCGATAAGG GCGAACTGAG CGAGATGATG 
CGATCGCTGG TGGAGATTGC GAGGACCACG AGCCTGGGTG ATGACCAGGT CACGGAACTG 
ATCGATGGCA TGTTTCAGGA TGTGGGGCTG GAGCACAAGA ACCATCTGAC CTACCAGGAC 
TTCAAACTGA TGATGAAGGA GTACAAGGGT GACTTTGTGG CCATCGGACT GGACTGCAAG 
GGAGCCAAGC AGAACTTCCT GGACACCTCG ACGAACGTGG CCCGGATGAC TTCGTTCAAC 
ATCGAACCGA TGCAGGACAA GCCGCGTCAC TGGTTGCTGG CCAAATGGGA TGCCTACATC 
ACGTTCCTCG AGGAGAACCG GCAGAACATC TTCTATCTGT TCCTCTTCTA CGTGGTCACC 
ATAGTTCTGT TCGTTGAGCG CTTCATCCAC TACTCGTTCA TGGCGGAGCA CACCGATCTG 
CGGCACATTA TGGGCGTAGG CATTGCCATC ACCAGGGGAT CTGCCGCATC GCTGTCCTTC 
TGCTACTCCC TGCTGCTGCT GACCATGTCT CGGAATCTGA TCACCAAGCT GAAGGAGTTC 
CCCATTCAAC AGTACATTCC CCTGGACTCG CACATACAGT TCCACAAGAT TGCCGCCTGC 
ACGGCGCTCT TCTTTTCCGT GCTGCACACG GTGGGCCACA TAGTGAACTT CTATCACGTG 
TCCACCCAAT CGCACGAGAA CCTGCGCTGC CTGACCCGCG AAGTGCACTT CGCCTCCGAC 
TACAAGCCGG ATATCACCTT CTGGCTCTTC CAGACGGTCA CCGGCACCAC AGGCGTCATG 
TTGTTCATCA TCATGTGCAT CATCTTCGTG TTCGCTCATC CTACGATCCG CAAGAAGGCC 
TACAACTTCT TCTGGAACAT GCACACCCTG TACATCGGAT TGTATCTTCT GAGTCTGATC 
CACGGATTGG CTCGACTGAC TGGACCACCA CGCTTCTGGA TGTTCTTCCT GGGCCCCGGA 
ATCGTGTACA CGCTGGATAA GATCGTCTCG CTGCGCACCA AGTACATGGC GCTGGACGTG 
ATCGACACAG ATCTGCTGCC CTCCGACGTG ATCAAGATCA AGTTCTATCG GCCACCGAAT 
CTAAAATACC TTTCTGGCCA ATGGGTGCGG TTGTCCTGTA CCGCCTTCCG GCCGCACGAG 
ATGCACAGCT TCACGCTGAC CTCGGCGCCG CACGAGAACT TCCTCAGCTG CCACATCAAG 
GCCCAAGGTC CGTGGACGTG GAAGCTGCGC AATTACTTCG ATCCCTGCAA CTACAATCCG 
GAGGACCAGC CCAAGATCCG CATCGAGGGA CCATTTGGTG GTGGCAACCA GGACTGGTAC 
AAGTTCGAGG TGGCTGTGAT GGTGGGCGGC GGCATCGGGG TCACTCCATA CGCCTCCATT 
CTCAACGACC TGGTCTTTGG CACCAGTACC AATCGATACT CGGGCGTGGC CTGCAAGAAG 
GTGTACTTCC TGTGGATCTG CCCATCGCAC AAGCACTTTG AGTGGTTCAT CGACGTCCTG 
CGCGATGTGG AGAAGAAGGA TGTGACCAAT GTGCTGGAGA TACACATATT CATCACGCAG 
TTCTTTCACA AGTTCGATCT GCGAACAACA ATGCTGTACA TCTGTGAGAA CCACTTCCAG 
CGACTGTCGA AGACCTCGAT ATTCACGGGT CTCAAGGCGG TGAATCACTT TGGTCGCCCA 
GATATGTCCA GCTTCTTGAA GTTCGTGCAA AAGAAGCACT CATACGTCTC CAAGATAGGA 
GTCTTCTCCT GCGGTCCTCG TCCGCTGACC AAGAGCGTGA TGTCTGCCTG TGATGAAGTG 
AACAAGACGC GCAAGTTGCC CTATTTCATC CACCACTTCG AGAACTTTGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608715.2
CDS	351..4964
Translation	MSVPSAPHQRAESKNRVPRPGQKNRKLPKLRLHWPGATYGGALLLLLISYGLELGSVHCYEKMYSQTEKQRYDGWYNNLAHPDWGSVDSHLVRKAPPSYSDGVYAMAGANRPSTRRLSRLFMRGKDGLGSKFNRTALLAFFGQLVANEIVMASESGCPIEMHRIEIEKCDEMYDRECRGDKYIPFHRAAYDRDTGQSPNAPREQINQMTAWIDGSFIYSTSEAWLNAMRSFHNGTLLTEKDGKLPVRNTMRVPLFNNPVPSVMKMLSPERLFLLGDPRTNQNPAILSFAILFLRWHNTLAQRIKRVHPDWSDEDIYQRARHTVIASLQNVIVYEYLPAFLGTSLPPYEGYKQDIHPGIGHIFQAAAFRFGHTMIPPGIYRRDGQCNFKETPMGYPAVRLCSTWWDSSGFFADTSVEEVLMGLASQISEREDPVLCSDVRDKLFGPMEFTRRDLGALNIMRGRDNGLPDYNTARESYGLKRHKTWTDINPPLFETQPELLDMLKEAYDNKLDDVDVYVGGMLESYGQPGEFFTAVIKEQFQRLRDADRFWFENERNGIFTPEEIAELRKITLWDIIVNSTDVKEEEIQKDVFMWRTGDPCPQPMQLNATELEPCTYLEGYDYFSGSELMFIYVCVFLGFVPILCAGAGYCVVKLQNSKRRRLKIRQEALRAPQHKGSVDKMLAREWLHANHKRLVTVKFGPEAAIYTVDRKGEKLRTFSLKHIDVVSVEESATNHIKKKPYILLRVPSDHDLVLELESYGARRKFVKKLEDFLLLHKKEMTLMEVNRDIMLARAETRERRQKRLEYFFREAYALTFGLRPGERRRRSDASSDGEVMTVMRTSLSKAEFAAALGMKPNDMFVRKMFNIVDKDQDGRISFQEFLETVVLFSRGKTDDKLRIIFDMCDNDRNGVIDKGELSEMMRSLVEIARTTSLGDDQVTELIDGMFQDVGLEHKNHLTYQDFKLMMKEYKGDFVAIGLDCKGAKQNFLDTSTNVARMTSFNIEPMQDKPRHWLLAKWDAYITFLEENRQNIFYLFLFYVVTIVLFVERFIHYSFMAEHTDLRHIMGVGIAITRGSAASLSFCYSLLLLTMSRNLITKLKEFPIQQYIPLDSHIQFHKIAACTALFFSVLHTVGHIVNFYHVSTQSHENLRCLTREVHFASDYKPDITFWLFQTVTGTTGVMLFIIMCIIFVFAHPTIRKKAYNFFWNMHTLYIGLYLLSLIHGLARLTGPPRFWMFFLGPGIVYTLDKIVSLRTKYMALDVIDTDLLPSDVIKIKFYRPPNLKYLSGQWVRLSCTAFRPHEMHSFTLTSAPHENFLSCHIKAQGPWTWKLRNYFDPCNYNPEDQPKIRIEGPFGGGNQDWYKFEVAVMVGGGIGVTPYASILNDLVFGTSTNRYSGVACKKVYFLWICPSHKHFEWFIDVLRDVEKKDVTNVLEIHIFITQFFHKFDLRTTMLYICENHFQRLSKTSIFTGLKAVNHFGRPDMSSFLKFVQKKHSYVSKIGVFSCGPRPLTKSVMSACDEVNKTRKLPYFIHHFENFG

Target ORF information:

RefSeq Version	NM_134871.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dual oxidase, transcript variant B (Duox), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134871.3

ORF Insert Sequence:

ATGAGTGTTC CAAGTGCGCC CCATCAGAGG GCAGAAAGCA AAAATCGAGT GCCGCGTCCG 
GGCCAAAAAA ACCGCAAGCT CCCCAAGCTC CGGCTCCACT GGCCGGGGGC AACCTACGGA 
GGAGCCCTGC TGCTTCTACT GATTAGTTAC GGTCTGGAGC TGGGCTCCGT GCATTGTTAT 
GAGAAAATGT ATAGTCAGAC CGAGAAACAG CGCTACGATG GCTGGTACAA TAACCTGGCC 
CATCCCGACT GGGGATCCGT GGATAGCCAC CTGGTGCGCA AGGCGCCGCC CTCCTATTCG 
GATGGGGTTT ACGCAATGGC AGGTGCCAAC CGGCCCTCGA CCCGCAGGCT CAGCCGGCTG 
TTTATGCGCG GAAAGGACGG CCTGGGGTCA AAGTTCAACC GGACTGCACT ACTGGCCTTC 
TTCGGCCAGC TGGTGGCCAA CGAGATAGTG ATGGCCTCGG AGTCCGGCTG CCCCATCGAG 
ATGCACCGCA TCGAGATCGA GAAGTGTGAC GAGATGTACG ACCGGGAGTG CCGTGGCGAC 
AAGTACATAC CATTCCATCG GGCGGCCTAC GATCGCGATA CCGGGCAGAG CCCGAATGCG 
CCGAGGGAGC AGATAAATCA AATGACTGCT TGGATTGATG GCAGTTTCAT TTACAGCACC 
TCCGAGGCCT GGCTCAACGC CATGCGCTCC TTCCACAATG GCACCCTGCT CACCGAGAAG 
GATGGGAAGC TTCCGGTGCG GAACACCATG CGGGTGCCGC TCTTCAACAA TCCGGTGCCG 
AGTGTCATGA AGATGCTCAG TCCGGAGCGG CTGTTTCTTC TTGGTGATCC TCGAACCAAT 
CAGAACCCGG CGATCCTCTC CTTCGCCATT CTCTTTCTGC GTTGGCACAA CACCCTGGCC 
CAGCGAATCA AGCGGGTGCA TCCGGATTGG AGCGATGAGG ATATCTATCA GCGAGCACGC 
CACACGGTGA TTGCCAGTCT TCAGAATGTG ATAGTCTACG AGTACCTGCC CGCCTTCCTG 
GGCACCTCGT TGCCACCTTA CGAGGGCTAC AAGCAGGACA TACATCCTGG CATTGGCCAC 
ATCTTCCAGG CGGCCGCCTT TCGGTTCGGC CACACGATGA TTCCGCCGGG AATTTACAGG 
CGAGATGGTC AGTGCAACTT TAAGGAAACG CCCATGGGTT ACCCGGCGGT CAGACTCTGC 
TCCACTTGGT GGGACTCCAG CGGATTTTTT GCCGACACCA GCGTAGAGGA GGTACTAATG 
GGCTTGGCTT CGCAAATATC CGAACGTGAG GATCCGGTTC TCTGTTCAGA TGTGCGGGAC 
AAACTTTTCG GACCGATGGA ATTCACACGC CGGGATCTGG GTGCTCTCAA CATAATGCGG 
GGTCGGGACA ATGGTCTGCC AGATTACAAT ACCGCGAGGG AGTCTTATGG ACTGAAACGG 
CACAAGACCT GGACGGACAT CAATCCTCCG TTGTTCGAAA CGCAGCCGGA ACTCTTGGAC 
ATGCTGAAGG AGGCGTACGA TAACAAGCTG GATGATGTGG ATGTTTATGT GGGTGGTATG 
CTGGAATCCT ACGGTCAACC GGGCGAGTTC TTCACGGCCG TAATCAAGGA GCAGTTCCAG 
CGACTACGAG ATGCCGATAG ATTTTGGTTC GAGAACGAGC GCAACGGCAT ATTTACTCCC 
GAGGAGATCG CTGAGCTACG CAAGATCACC CTGTGGGACA TCATAGTGAA CAGCACGGAT 
GTGAAGGAGG AGGAGATCCA AAAGGATGTG TTCATGTGGC GCACTGGCGA TCCCTGTCCG 
CAGCCAATGC AGCTGAATGC CACCGAACTG GAGCCGTGCA CTTACTTGGA GGGCTACGAC 
TACTTCTCCG GCTCGGAGCT GATGTTTATC TACGTTTGTG TCTTCCTTGG ATTCGTGCCC 
ATATTGTGTG CCGGAGCCGG TTACTGTGTG GTCAAGCTGC AGAACAGCAA GCGCCGTCGG 
CTGAAGATCC GTCAGGAGGC ATTGAGGGCA CCGCAGCACA AGGGATCCGT GGACAAGATG 
CTGGCCAGGG AGTGGCTGCA CGCCAACCAC AAGAGACTGG TCACGGTTAA ATTCGGACCA 
GAGGCGGCCA TCTACACGGT GGATAGGAAG GGCGAGAAGT TGCGCACCTT TAGTTTGAAG 
CACATCGACG TGGTCAGTGT GGAGGAGTCG GCCACGAACC ACATCAAGAA GAAACCTTAC 
ATCCTGCTGC GCGTGCCCAG CGATCATGAT CTGGTGCTCG AGCTGGAATC TTACGGAGCA 
CGCAGGAAGT TTGTAAAGAA GCTGGAGGAT TTCCTACTGC TCCACAAGAA GGAGATGACT 
TTGATGGAGG TCAACAGGGA CATAATGTTG GCGCGGGCGG AGACACGGGA GCGGCGCCAA 
AAGCGACTGG AGTACTTCTT CCGTGAGGCT TACGCCCTGA CCTTTGGCCT GAGACCCGGA 
GAACGACGAA GGCGTTCAGA TGCCTCCAGT GATGGCGAGG TGATGACCGT GATGCGGACC 
AGTCTTTCCA AGGCGGAATT CGCCGCCGCT CTGGGCATGA AGCCCAACGA CATGTTTGTG 
CGCAAGATGT TCAACATTGT GGACAAGGAT CAGGATGGCA GGATTAGCTT CCAGGAGTTC 
CTGGAGACCG TAGTTCTTTT CTCCCGCGGC AAAACGGACG ACAAGCTGCG CATAATCTTT 
GATATGTGTG ATAATGATCG GAATGGCGTC ATCGATAAGG GCGAACTGAG CGAGATGATG 
CGATCGCTGG TGGAGATTGC GAGGACCACG AGCCTGGGTG ATGACCAGGT CACGGAACTG 
ATCGATGGCA TGTTTCAGGA TGTGGGGCTG GAGCACAAGA ACCATCTGAC CTACCAGGAC 
TTCAAACTGA TGATGAAGGA GTACAAGGGT GACTTTGTGG CCATCGGACT GGACTGCAAG 
GGAGCCAAGC AGAACTTCCT GGACACCTCG ACGAACGTGG CCCGGATGAC TTCGTTCAAC 
ATCGAACCGA TGCAGGACAA GCCGCGTCAC TGGTTGCTGG CCAAATGGGA TGCCTACATC 
ACGTTCCTCG AGGAGAACCG GCAGAACATC TTCTATCTGT TCCTCTTCTA CGTGGTCACC 
ATAGTTCTGT TCGTTGAGCG CTTCATCCAC TACTCGTTCA TGGCGGAGCA CACCGATCTG 
CGGCACATTA TGGGCGTAGG CATTGCCATC ACCAGGGGAT CTGCCGCATC GCTGTCCTTC 
TGCTACTCCC TGCTGCTGCT GACCATGTCT CGGAATCTGA TCACCAAGCT GAAGGAGTTC 
CCCATTCAAC AGTACATTCC CCTGGACTCG CACATACAGT TCCACAAGAT TGCCGCCTGC 
ACGGCGCTCT TCTTTTCCGT GCTGCACACG GTGGGCCACA TAGTGAACTT CTATCACGTG 
TCCACCCAAT CGCACGAGAA CCTGCGCTGC CTGACCCGCG AAGTGCACTT CGCCTCCGAC 
TACAAGCCGG ATATCACCTT CTGGCTCTTC CAGACGGTCA CCGGCACCAC AGGCGTCATG 
TTGTTCATCA TCATGTGCAT CATCTTCGTG TTCGCTCATC CTACGATCCG CAAGAAGGCC 
TACAACTTCT TCTGGAACAT GCACACCCTG TACATCGGAT TGTATCTTCT GAGTCTGATC 
CACGGATTGG CTCGACTGAC TGGACCACCA CGCTTCTGGA TGTTCTTCCT GGGCCCCGGA 
ATCGTGTACA CGCTGGATAA GATCGTCTCG CTGCGCACCA AGTACATGGC GCTGGACGTG 
ATCGACACAG ATCTGCTGCC CTCCGACGTG ATCAAGATCA AGTTCTATCG GCCACCGAAT 
CTAAAATACC TTTCTGGCCA ATGGGTGCGG TTGTCCTGTA CCGCCTTCCG GCCGCACGAG 
ATGCACAGCT TCACGCTGAC CTCGGCGCCG CACGAGAACT TCCTCAGCTG CCACATCAAG 
GCCCAAGGTC CGTGGACGTG GAAGCTGCGC AATTACTTCG ATCCCTGCAA CTACAATCCG 
GAGGACCAGC CCAAGATCCG CATCGAGGGA CCATTTGGTG GTGGCAACCA GGACTGGTAC 
AAGTTCGAGG TGGCTGTGAT GGTGGGCGGC GGCATCGGGG TCACTCCATA CGCCTCCATT 
CTCAACGACC TGGTCTTTGG CACCAGTACC AATCGATACT CGGGCGTGGC CTGCAAGAAG 
GTGTACTTCC TGTGGATCTG CCCATCGCAC AAGCACTTTG AGTGGTTCAT CGACGTCCTG 
CGCGATGTGG AGAAGAAGGA TGTGACCAAT GTGCTGGAGA TACACATATT CATCACGCAG 
TTCTTTCACA AGTTCGATCT GCGAACAACA ATGCTGTACA TCTGTGAGAA CCACTTCCAG 
CGACTGTCGA AGACCTCGAT ATTCACGGGT CTCAAGGCGG TGAATCACTT TGGTCGCCCA 
GATATGTCCA GCTTCTTGAA GTTCGTGCAA AAGAAGCACT CATACGTCTC CAAGATAGGA 
GTCTTCTCCT GCGGTCCTCG TCCGCTGACC AAGAGCGTGA TGTCTGCCTG TGATGAAGTG 
AACAAGACGC GCAAGTTGCC CTATTTCATC CACCACTTCG AGAACTTTGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Duox cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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