l(2)k05819 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(2)k05819
Entrez Gene ID	33644
Full Name	lethal (2) k05819
Synonyms	CG3054,Dmel\CG3054,anon-WO0140519.156
General protein information	Preferred Names lethal (2) k05819 Names CG3054 gene product from transcript CG3054-RD CG3054-PA CG3054-PC CG3054-PD l(2)k05819-PA l(2)k05819-PC l(2)k05819-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24E5-24F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273094.1	NP_001260023.1	lethal (2) k05819, isoform D
NM_164582.3	NP_722974.1	lethal (2) k05819, isoform A
NM_134985.4	NP_608829.2	lethal (2) k05819, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	l(2)k05819	NP_722974.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000576	XP_310498.5
Homo sapiens (human)	KIAA0195	NP_055553.3
Pan troglodytes (chimpanzee)	KIAA0195	XP_001142508.1
Gallus gallus (chicken)	KIAA0195	XP_004946429.1
Bos taurus (cattle)	KIAA0195	XP_005221370.1
Mus musculus (house mouse)	2310067B10Rik	NP_082290.2
	KIAA0195	XP_001096873.1
Canis lupus familiaris (dog)	KIAA0195	XP_540432.3
Danio rerio (zebrafish)	LOC795408	XP_005169540.1
Caenorhabditis elegans (roundworm)	D1007.15	NP_491390.2
Xenopus tropicalis (tropical clawed frog)	kiaa0195	XP_004918526.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(2)k05819 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(2)k05819 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13725	NM_164582.3 Latest version!	Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13726	NM_134985.4 Latest version!	Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13726	NM_001273094.1 Latest version!	Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(2)k05819 ( NM_164582.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722974.1 Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13725
Clone ID Related Accession (Same CDS sequence)	NM_164582.3
Accession Version	NM_164582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) k05819, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164582.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGA AGCGGGCGGA AAGTCCGGAG AAGCGGGCTA GTCCGGAGCC CACTCTGCGT 
GGACTAACCA CAAAGGTGGC GCTGCGGCGT CTACATGAGG AGATTGAAAA ACGGGTGCAA 
CTGGAGCACG AGCGGCAATA CGGAGCGGAG AGCCCCTATC GAAAATTGCG GGCGGCCTTT 
GTGAAGCGGT ACGATAGCCC ACTGGGATGG CTTTCCATTG GAAGCAGTTT GCTGGCTAGT 
GGAGCACTGC TCGCTACAGG AATGCTATGG CCCGCCTTGT GCCTGCTGGC CATCTTATCG 
CTGGACCTCT TTGTGGTGGT CCGGGAGAAC AACCTGCGGC GCACAGAGAT CTTCCGGAAG 
GCACGACACG CGATTTCGGA AATTAAACTA GCGGAACAGC ACTTCAGTGA CGATTGGCAG 
CAATCCAACT ATCCGCACCT AAGTAATCCC ATGTCGCCCT GCGTTTCCCT GCAGTGGACC 
TACCGAGATG GAAAAATTGT CAACTTGCCG TGGGCTTTGT TGGTGCGCGG GGATTACATC 
GTTCTACGGC CGGGACACAT TTCACCCGGA CCTTGTCACG AACTGGAGAC AAATAAATTT 
TTTGCTGCTA ACGAAATATA TGGAGCTGCT CCTCACGGAG ATCCTCCAGT GAAACCTGTG 
GCACGACCTC CGCTGCCCAA TCTCGTTTGT TGTTTGGAAA GCACACCTTA CCTGGATAAC 
CTTACTGTTT GCCTCAAAAA CTCTCTGAAG CGACCGCCGA CCATCTACAA TCAGCAGCGC 
TATCTGCTGG TCACCAAGTA TATACAGCAG TGGGGCTTCA TTAGTGCCCT AGTTATCACG 
CTCATCTCGG GGTTACTGCG CCTCCATGGC TATGGCCTGC CTACCGATGA GAAGCACAAC 
TGGCGCTTTG TGCTCGTCGA ATCCTCGGCG GCGGCCATAG TTCCCCTATT GCCGCTTATC 
TTCCCGCTCA TGTGGATATC CATGAACCTG TGGGGAATAG CTCGATTGCA GTGTTTTCTA 
AAGACGCCTC AGGCTGCGTT GGAAAAGAGT ACAAGCAAAT CGTTCGAAGA GGACCTGGAC 
ACGCCCTCGT ACGACTATCA GAATATGTCC ATGCTTCGTT CCAGTGTGCT CCGCATCTGG 
ATGCAGCTGT GGCGTGGTGA CAGTGAACTC CTGCCGCGCT CAGCCAATCT GGTACAGGTG 
CTGGGGTCCA TTTCGGCGCT GTGCTGCGTG GACAAGAAAG GTATCCTGTC GTGGCCCAAT 
CCCACCGCCG AAAAGGTATT CTTCCTGCAC AACACAGACG AGGAGGCTCG CGAGGATGGA 
GCAGGCAGTC AGTCTTCTTC ACAGAGCGAG AGCGACGACG TACCGGTTGT GGATGAGGAC 
CACAAGGAGC GCGGAATAGT GGCTGAAGTG CTGGACCTCA CGCACGACCA ACACTGCCCC 
TTTCGGCTGG AGTTCGACGA TCACGAATGG AAGGCGCACA TCAAGTCCTT GAAGCCACTG 
GGTCTTGCCA TTCTGCTAAA CACCTGTTCT CCACTCACCC ACGAGCACTA CGCACAGTTC 
TGCGGTCATG TAACCGCGGT GGCTATGCTC GACAAAGATC TGGTACCTGT GACTAATCGG 
TATGCGTATG CACTCATTGA GTCCAGTAAA AGCTTTTTGC ATGGACGCTG CCTTTGCGAA 
CTGGCCAAAC AGATTGGATT CACCGACCAT GCCACTGATC GGTTCGCGCT GGAGGGTCAA 
TTGGCTAGCT ATCGCCACTT GCAACCTGAT GTTGTGCGAA GGGACATTCG TTTCGCCCGG 
CAGCTCCAGC TGTCCTCCAA GGTGAAGGTC CCATTCCCGC ACTCTTTGTC TGTGGTCATG 
CGCGAGAATC CAGGCGGCTA TTTATCACTT TTCACCCAGG GAACAGCAGA CATTATACTG 
GACTGCTGCG ATGACTTTTG GGACGGCATT GATCTGCGAC CATTGTCCGC ACAGGATCGC 
AAGCGTGCAC TGGACTTTTA TCAGCGCAGC GCGCTGACGT CCTATTGTAC AGCGTTTGCC 
TATCGACCTC TAAGGCACGG CATCCATGGA GCCCTTAGCG GTCCTCAACG GAGTGGAGGT 
GACTGTTTGT ATCTGGAGCT ACCGCCGGAG TCAAAGCTGC GCATGCAGCA CGTGGACAAG 
ACCCATTGCG ACTTCCAGGG CGGACATCAC GTCAAGCTGA GCCACAGCAT TAGCACGGAC 
TCATTGCTAT TTTCGGAGAA CAAGGACGAG GAGTGTAACG ATGTGGAGGG CTGCTTCGAG 
ATGCAGTGCC ATCAGGTATT CATCGGCATG GTGACAATGC AGTACCAGGC GCAAAACGAT 
ATCGTACGAC TCGTTGAGCA TCTCGAACAG GCCTGCATAC GCTTTGTTCA CTTTAGCAAA 
GAAAACGAAC TGCGCTCGCG TGTCTTCTCT GAGAAAATGG GTCTAGAGTC CGGATGGAAC 
TGCCACATCT CGCTACTGAG CGAACCTGAG CCGGATAGTG AAAAGGAGCG GATAAGTGCC 
AAGCCAAAGG AGTTCCCGGA AAATATTGGT AAACCCCAAG CCACCACCTC AACCACTGCC 
ACCACCACTA CAACAGACCA TGAGAACAAC CATCATCCCA GTGCCAAAGT CATGTACATA 
TCGCCCCCCG CACCCGCACC TGCCACTGCA TCTGTATCCG AATCCATATC TGCAGCTAAA 
ACCACTGAAA TTGTAGGTGA CGGCCCAGAA ATTAGTTATC TCTTGGGACC GCCAAACAAT 
CTGGAGTCGT CAAAGACCCT GAGCTCTTCA GCTCCTGGTG CCATTTCCAA TCCGGATGAG 
AATCCACTTA ACGCCCTCAA TAGTGGGGAT TCCCATTCAC CTGATAGCGA GGGCAGTAAG 
GAGAGCTCTC AGGATAGCTG CACAAAGAAT AACCCACCAG GCGATTCTGA GTTGGAGGCG 
GAAACCGAGG CGAACAAGCA CAAGCAGCGC CATTCGCTGG ACTCTGCCAT GGACGAAAAC 
TGCCGTTCGT TGAGCACACT CACTGAAAGC ACGGATCAGA GTGCACCCAT CAACTTCGAT 
ATGTCAAATA GGGCAAAGCT GCCAAGGGGC ATTGAAAACA TTCGGCCGCA TTTGGAACAG 
GTTGACAATG TCCCACTGCA AGTATCGCTT TTTACGGACT GCTCAGCGGA AGCCACGCGT 
CAGATGCTGG ACATCATGCA ATCCTACGGC GAGATTGTTG TCTGCCTTGG AAGTTCGGCC 
AGTAATGCCA ATGCGGACAT TTTTCTGCAG GCCGACTGTA GTATAGCCGT GGAGCCGTTG 
TATCCGCAAG TGTGCCAGGA TGTGGACGCG TACACGGAAG CCAATATCCA GAACAACAAA 
CTGTTGTGGC AGCGGCATTC CGGCAAGTCA AAGGGAAACG ATGATGAGGA GCTTCTGGAG 
GGCGGCCTTC ATGGATGCGC CATGCAGTCG CCAGCTCACA CCATCTCGCC ACTGTACTTA 
AGCCGGCTGC TCAACTCTCT GCCCTGTTCC ATTGCCGTTT GCCGCGACGA CCCGCTTTCG 
CTGGTGGCCA TAATTGAGCT TTCACGTCGA TTCTCTCTGG GCCTGTGGAA CTGCATCCAG 
TACTGGGCTT GCTGCGCAGG CTCTATCTCC ATTCTGAACG TGCTGTGCGC GTGCCTCTCG 
CAACCCCCGC TCGTAACGCC GCTGCTCGGC ATTTATCTGA TGTGTGTGCC GGTGCCGCTA 
ATAGCCATTT CGCTAGCACA CATCGACGTG GATCCCAAAA TCATGAATCG GGCAACAAGC 
AAAAAGCAGA CGGACTATGA TTCTCGCGCC TTTGCCTTTG TCATGTGGTG CTACGGCTGC 
AAGTTTATTG TACTGGTCGT GTCTCTGCTT GTTTCCTATT GGATGCTCTT ACCAAACACC 
CAATTAAATC CCGCTGAACC AGCCACTGAG GAACCCGTGT TGGAAATCGA CGATGATGAC 
GACCCGGAGT TTTATCAATT TCTTTACATT GCTAGAAGCT CTGCGCTGCT AGGCATAATC 
CTACACTTCG TTTTCATTTC TGTCACCTTT GTGCATCGAG ACCACGCGCT CTGGCAGCGC 
AATCCATTTA CCAATCGTAT TTGGGCTTTT ACATGTTTGT TCTTAGTCCT CATCCATGGT 
ATTATTTGCT TTGTGCAACT GCTGCTGGAG GATTTCTGGG TTCAGCTGCC CAACCAGTGG 
TTGGCCTTGT TAATCTTATT TGGTGGCAGC TTGCTGAGTA TGCTCGTCAG CGAGCTGGCG 
AAATTCCAGG AGAACAAGGT TAACGCGCGC TATCAGCGTC GGGCGCGTCT TGACTTCGGT 
ACCAAATTGG GCATGAATTC GCCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722974.1
CDS	143..4492
Translation	MAAKRAESPEKRASPEPTLRGLTTKVALRRLHEEIEKRVQLEHERQYGAESPYRKLRAAFVKRYDSPLGWLSIGSSLLASGALLATGMLWPALCLLAILSLDLFVVVRENNLRRTEIFRKARHAISEIKLAEQHFSDDWQQSNYPHLSNPMSPCVSLQWTYRDGKIVNLPWALLVRGDYIVLRPGHISPGPCHELETNKFFAANEIYGAAPHGDPPVKPVARPPLPNLVCCLESTPYLDNLTVCLKNSLKRPPTIYNQQRYLLVTKYIQQWGFISALVITLISGLLRLHGYGLPTDEKHNWRFVLVESSAAAIVPLLPLIFPLMWISMNLWGIARLQCFLKTPQAALEKSTSKSFEEDLDTPSYDYQNMSMLRSSVLRIWMQLWRGDSELLPRSANLVQVLGSISALCCVDKKGILSWPNPTAEKVFFLHNTDEEAREDGAGSQSSSQSESDDVPVVDEDHKERGIVAEVLDLTHDQHCPFRLEFDDHEWKAHIKSLKPLGLAILLNTCSPLTHEHYAQFCGHVTAVAMLDKDLVPVTNRYAYALIESSKSFLHGRCLCELAKQIGFTDHATDRFALEGQLASYRHLQPDVVRRDIRFARQLQLSSKVKVPFPHSLSVVMRENPGGYLSLFTQGTADIILDCCDDFWDGIDLRPLSAQDRKRALDFYQRSALTSYCTAFAYRPLRHGIHGALSGPQRSGGDCLYLELPPESKLRMQHVDKTHCDFQGGHHVKLSHSISTDSLLFSENKDEECNDVEGCFEMQCHQVFIGMVTMQYQAQNDIVRLVEHLEQACIRFVHFSKENELRSRVFSEKMGLESGWNCHISLLSEPEPDSEKERISAKPKEFPENIGKPQATTSTTATTTTTDHENNHHPSAKVMYISPPAPAPATASVSESISAAKTTEIVGDGPEISYLLGPPNNLESSKTLSSSAPGAISNPDENPLNALNSGDSHSPDSEGSKESSQDSCTKNNPPGDSELEAETEANKHKQRHSLDSAMDENCRSLSTLTESTDQSAPINFDMSNRAKLPRGIENIRPHLEQVDNVPLQVSLFTDCSAEATRQMLDIMQSYGEIVVCLGSSASNANADIFLQADCSIAVEPLYPQVCQDVDAYTEANIQNNKLLWQRHSGKSKGNDDEELLEGGLHGCAMQSPAHTISPLYLSRLLNSLPCSIAVCRDDPLSLVAIIELSRRFSLGLWNCIQYWACCAGSISILNVLCACLSQPPLVTPLLGIYLMCVPVPLIAISLAHIDVDPKIMNRATSKKQTDYDSRAFAFVMWCYGCKFIVLVVSLLVSYWMLLPNTQLNPAEPATEEPVLEIDDDDDPEFYQFLYIARSSALLGIILHFVFISVTFVHRDHALWQRNPFTNRIWAFTCLFLVLIHGIICFVQLLLEDFWVQLPNQWLALLILFGGSLLSMLVSELAKFQENKVNARYQRRARLDFGTKLGMNSPF

Target ORF information:

RefSeq Version	NM_164582.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164582.3

ORF Insert Sequence:

ATGGCAGCGA AGCGGGCGGA AAGTCCGGAG AAGCGGGCTA GTCCGGAGCC CACTCTGCGT 
GGACTAACCA CAAAGGTGGC GCTGCGGCGT CTACATGAGG AGATTGAAAA ACGGGTGCAA 
CTGGAGCACG AGCGGCAATA CGGAGCGGAG AGCCCCTATC GAAAATTGCG GGCGGCCTTT 
GTGAAGCGGT ACGATAGCCC ACTGGGATGG CTTTCCATTG GAAGCAGTTT GCTGGCTAGT 
GGAGCACTGC TCGCTACAGG AATGCTATGG CCCGCCTTGT GCCTGCTGGC CATCTTATCG 
CTGGACCTCT TTGTGGTGGT CCGGGAGAAC AACCTGCGGC GCACAGAGAT CTTCCGGAAG 
GCACGACACG CGATTTCGGA AATTAAACTA GCGGAACAGC ACTTCAGTGA CGATTGGCAG 
CAATCCAACT ATCCGCACCT AAGTAATCCC ATGTCGCCCT GCGTTTCCCT GCAGTGGACC 
TACCGAGATG GAAAAATTGT CAACTTGCCG TGGGCTTTGT TGGTGCGCGG GGATTACATC 
GTTCTACGGC CGGGACACAT TTCACCCGGA CCTTGTCACG AACTGGAGAC AAATAAATTT 
TTTGCTGCTA ACGAAATATA TGGAGCTGCT CCTCACGGAG ATCCTCCAGT GAAACCTGTG 
GCACGACCTC CGCTGCCCAA TCTCGTTTGT TGTTTGGAAA GCACACCTTA CCTGGATAAC 
CTTACTGTTT GCCTCAAAAA CTCTCTGAAG CGACCGCCGA CCATCTACAA TCAGCAGCGC 
TATCTGCTGG TCACCAAGTA TATACAGCAG TGGGGCTTCA TTAGTGCCCT AGTTATCACG 
CTCATCTCGG GGTTACTGCG CCTCCATGGC TATGGCCTGC CTACCGATGA GAAGCACAAC 
TGGCGCTTTG TGCTCGTCGA ATCCTCGGCG GCGGCCATAG TTCCCCTATT GCCGCTTATC 
TTCCCGCTCA TGTGGATATC CATGAACCTG TGGGGAATAG CTCGATTGCA GTGTTTTCTA 
AAGACGCCTC AGGCTGCGTT GGAAAAGAGT ACAAGCAAAT CGTTCGAAGA GGACCTGGAC 
ACGCCCTCGT ACGACTATCA GAATATGTCC ATGCTTCGTT CCAGTGTGCT CCGCATCTGG 
ATGCAGCTGT GGCGTGGTGA CAGTGAACTC CTGCCGCGCT CAGCCAATCT GGTACAGGTG 
CTGGGGTCCA TTTCGGCGCT GTGCTGCGTG GACAAGAAAG GTATCCTGTC GTGGCCCAAT 
CCCACCGCCG AAAAGGTATT CTTCCTGCAC AACACAGACG AGGAGGCTCG CGAGGATGGA 
GCAGGCAGTC AGTCTTCTTC ACAGAGCGAG AGCGACGACG TACCGGTTGT GGATGAGGAC 
CACAAGGAGC GCGGAATAGT GGCTGAAGTG CTGGACCTCA CGCACGACCA ACACTGCCCC 
TTTCGGCTGG AGTTCGACGA TCACGAATGG AAGGCGCACA TCAAGTCCTT GAAGCCACTG 
GGTCTTGCCA TTCTGCTAAA CACCTGTTCT CCACTCACCC ACGAGCACTA CGCACAGTTC 
TGCGGTCATG TAACCGCGGT GGCTATGCTC GACAAAGATC TGGTACCTGT GACTAATCGG 
TATGCGTATG CACTCATTGA GTCCAGTAAA AGCTTTTTGC ATGGACGCTG CCTTTGCGAA 
CTGGCCAAAC AGATTGGATT CACCGACCAT GCCACTGATC GGTTCGCGCT GGAGGGTCAA 
TTGGCTAGCT ATCGCCACTT GCAACCTGAT GTTGTGCGAA GGGACATTCG TTTCGCCCGG 
CAGCTCCAGC TGTCCTCCAA GGTGAAGGTC CCATTCCCGC ACTCTTTGTC TGTGGTCATG 
CGCGAGAATC CAGGCGGCTA TTTATCACTT TTCACCCAGG GAACAGCAGA CATTATACTG 
GACTGCTGCG ATGACTTTTG GGACGGCATT GATCTGCGAC CATTGTCCGC ACAGGATCGC 
AAGCGTGCAC TGGACTTTTA TCAGCGCAGC GCGCTGACGT CCTATTGTAC AGCGTTTGCC 
TATCGACCTC TAAGGCACGG CATCCATGGA GCCCTTAGCG GTCCTCAACG GAGTGGAGGT 
GACTGTTTGT ATCTGGAGCT ACCGCCGGAG TCAAAGCTGC GCATGCAGCA CGTGGACAAG 
ACCCATTGCG ACTTCCAGGG CGGACATCAC GTCAAGCTGA GCCACAGCAT TAGCACGGAC 
TCATTGCTAT TTTCGGAGAA CAAGGACGAG GAGTGTAACG ATGTGGAGGG CTGCTTCGAG 
ATGCAGTGCC ATCAGGTATT CATCGGCATG GTGACAATGC AGTACCAGGC GCAAAACGAT 
ATCGTACGAC TCGTTGAGCA TCTCGAACAG GCCTGCATAC GCTTTGTTCA CTTTAGCAAA 
GAAAACGAAC TGCGCTCGCG TGTCTTCTCT GAGAAAATGG GTCTAGAGTC CGGATGGAAC 
TGCCACATCT CGCTACTGAG CGAACCTGAG CCGGATAGTG AAAAGGAGCG GATAAGTGCC 
AAGCCAAAGG AGTTCCCGGA AAATATTGGT AAACCCCAAG CCACCACCTC AACCACTGCC 
ACCACCACTA CAACAGACCA TGAGAACAAC CATCATCCCA GTGCCAAAGT CATGTACATA 
TCGCCCCCCG CACCCGCACC TGCCACTGCA TCTGTATCCG AATCCATATC TGCAGCTAAA 
ACCACTGAAA TTGTAGGTGA CGGCCCAGAA ATTAGTTATC TCTTGGGACC GCCAAACAAT 
CTGGAGTCGT CAAAGACCCT GAGCTCTTCA GCTCCTGGTG CCATTTCCAA TCCGGATGAG 
AATCCACTTA ACGCCCTCAA TAGTGGGGAT TCCCATTCAC CTGATAGCGA GGGCAGTAAG 
GAGAGCTCTC AGGATAGCTG CACAAAGAAT AACCCACCAG GCGATTCTGA GTTGGAGGCG 
GAAACCGAGG CGAACAAGCA CAAGCAGCGC CATTCGCTGG ACTCTGCCAT GGACGAAAAC 
TGCCGTTCGT TGAGCACACT CACTGAAAGC ACGGATCAGA GTGCACCCAT CAACTTCGAT 
ATGTCAAATA GGGCAAAGCT GCCAAGGGGC ATTGAAAACA TTCGGCCGCA TTTGGAACAG 
GTTGACAATG TCCCACTGCA AGTATCGCTT TTTACGGACT GCTCAGCGGA AGCCACGCGT 
CAGATGCTGG ACATCATGCA ATCCTACGGC GAGATTGTTG TCTGCCTTGG AAGTTCGGCC 
AGTAATGCCA ATGCGGACAT TTTTCTGCAG GCCGACTGTA GTATAGCCGT GGAGCCGTTG 
TATCCGCAAG TGTGCCAGGA TGTGGACGCG TACACGGAAG CCAATATCCA GAACAACAAA 
CTGTTGTGGC AGCGGCATTC CGGCAAGTCA AAGGGAAACG ATGATGAGGA GCTTCTGGAG 
GGCGGCCTTC ATGGATGCGC CATGCAGTCG CCAGCTCACA CCATCTCGCC ACTGTACTTA 
AGCCGGCTGC TCAACTCTCT GCCCTGTTCC ATTGCCGTTT GCCGCGACGA CCCGCTTTCG 
CTGGTGGCCA TAATTGAGCT TTCACGTCGA TTCTCTCTGG GCCTGTGGAA CTGCATCCAG 
TACTGGGCTT GCTGCGCAGG CTCTATCTCC ATTCTGAACG TGCTGTGCGC GTGCCTCTCG 
CAACCCCCGC TCGTAACGCC GCTGCTCGGC ATTTATCTGA TGTGTGTGCC GGTGCCGCTA 
ATAGCCATTT CGCTAGCACA CATCGACGTG GATCCCAAAA TCATGAATCG GGCAACAAGC 
AAAAAGCAGA CGGACTATGA TTCTCGCGCC TTTGCCTTTG TCATGTGGTG CTACGGCTGC 
AAGTTTATTG TACTGGTCGT GTCTCTGCTT GTTTCCTATT GGATGCTCTT ACCAAACACC 
CAATTAAATC CCGCTGAACC AGCCACTGAG GAACCCGTGT TGGAAATCGA CGATGATGAC 
GACCCGGAGT TTTATCAATT TCTTTACATT GCTAGAAGCT CTGCGCTGCT AGGCATAATC 
CTACACTTCG TTTTCATTTC TGTCACCTTT GTGCATCGAG ACCACGCGCT CTGGCAGCGC 
AATCCATTTA CCAATCGTAT TTGGGCTTTT ACATGTTTGT TCTTAGTCCT CATCCATGGT 
ATTATTTGCT TTGTGCAACT GCTGCTGGAG GATTTCTGGG TTCAGCTGCC CAACCAGTGG 
TTGGCCTTGT TAATCTTATT TGGTGGCAGC TTGCTGAGTA TGCTCGTCAG CGAGCTGGCG 
AAATTCCAGG AGAACAAGGT TAACGCGCGC TATCAGCGTC GGGCGCGTCT TGACTTCGGT 
ACCAAATTGG GCATGAATTC GCCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(2)k05819 ( NM_001273094.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260023.1 Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13726
Clone ID Related Accession (Same CDS sequence)	NM_001273094.1 , NM_134985.4
Accession Version	NM_001273094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) k05819, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGA AGCGGGCGGA AAGTCCGGAG AAGCGGGCTA GTCCGGAGCC CACTCTGCGT 
GGACTAACCA CAAAGGTGGC GCTGCGGCGT CTACATGAGG AGATTGAAAA ACGGGTGCAA 
CTGGAGCACG AGCGGCAATA CGGAGCGGAG AGCCCCTATC GAAAATTGCG GGCGGCCTTT 
GTGAAGCGGT ACGATAGCCC ACTGGGATGG CTTTCCATTG GAAGCAGTTT GCTGGCTAGT 
GGAGCACTGC TCGCTACAGG AATGCTATGG CCCGCCTTGT GCCTGCTGGC CATCTTATCG 
CTGGACCTCT TTGTGGTGGT CCGGGAGAAC AACCTGCGGC GCACAGAGAT CTTCCGGAAG 
GCACGACACG CGATTTCGGA AATTAAACTA GCGGAACAGC ACTTCAGTGA CGATTGGCAG 
CAATCCAACT ATCCGCACCT AAGTAATCCC ATGTCGCCCT GCGTTTCCCT GCAGTGGACC 
TACCGAGATG GAAAAATTGT CAACTTGCCG TGGGCTTTGT TGGTGCGCGG GGATTACATC 
GTTCTACGGC CGGGACACAT TTCACCCGGA CCTTGTCACG AACTGGAGAC AAATAAATTT 
TTTGCTGCTA ACGAAATATA TGGAGCTGCT CCTCACGGAG ATCCTCCAGT GAAACCTGTG 
GCACGACCTC CGCTGCCCAA TCTCGTTTGT TGTTTGGAAA GCACACCTTA CCTGGATAAC 
CTTACTGTTT GCCTCAAAAA CTCTCTGAAG CGACCGCCGA CCATCTACAA TCAGCAGCGC 
TATCTGCTGG TCACCAAGTA TATACAGCAG TGGGGCTTCA TTAGTGCCCT AGTTATCACG 
CTCATCTCGG GGTTACTGCG CCTCCATGGC TATGGCCTGC CTACCGATGA GAAGCACAAC 
TGGCGCTTTG TGCTCGTCGA ATCCTCGGCG GCGGCCATAG TTCCCCTATT GCCGCTTATC 
TTCCCGCTCA TGTGGATATC CATGAACCTG TGGGGAATAG CTCGATTGCA GTGTTTTCTA 
AAGACGCCTC AGGCTGCGTT GGAAAAGAGT ACAAGCAAAT CGTTCGAAGA GGACCTGGAC 
ACGCCCTCGT ACGACTATCA GAATATGTCC ATGCTTCGTT CCAGTGTGCT CCGCATCTGG 
ATGCAGCTGT GGCGTGGTGA CAGTGAACTC CTGCCGCGCT CAGCCAATCT GGTACAGGTG 
CTGGGGTCCA TTTCGGCGCT GTGCTGCGTG GACAAGAAAG GTATCCTGTC GTGGCCCAAT 
CCCACCGCCG AAAAGGTATT CTTCCTGCAC AACACAGACG AGGAGGCTCG CGAGGATGGA 
GCAGGCAGTC AGTCTTCTTC ACAGAGCGAG AGCGACGACG TACCGGTTGT GGATGAGGAC 
CACAAGGAGC GCGGAATAGT GGCTGAAGTG CTGGACCTCA CGCACGACCA ACACTGCCCC 
TTTCGGCTGG AGTTCGACGA TCACGAATGG AAGGCGCACA TCAAGTCCTT GAAGCCACTG 
GGTCTTGCCA TTCTGCTAAA CACCTGTTCT CCACTCACCC ACGAGCACTA CGCACAGTTC 
TGCGGTCATG TAACCGCGGT GGCTATGCTC GACAAAGATC TGGTACCTGT GACTAATCGA 
CGCTGCCTTT GCGAACTGGC CAAACAGATT GGATTCACCG ACCATGCCAC TGATCGGTTC 
GCGCTGGAGG GTCAATTGGC TAGCTATCGC CACTTGCAAC CTGATGTTGT GCGAAGGGAC 
ATTCGTTTCG CCCGGCAGCT CCAGCTGTCC TCCAAGGTGA AGGTCCCATT CCCGCACTCT 
TTGTCTGTGG TCATGCGCGA GAATCCAGGC GGCTATTTAT CACTTTTCAC CCAGGGAACA 
GCAGACATTA TACTGGACTG CTGCGATGAC TTTTGGGACG GCATTGATCT GCGACCATTG 
TCCGCACAGG ATCGCAAGCG TGCACTGGAC TTTTATCAGC GCAGCGCGCT GACGTCCTAT 
TGTACAGCGT TTGCCTATCG ACCTCTAAGG CACGGCATCC ATGGAGCCCT TAGCGGTCCT 
CAACGGAGTG GAGGTGACTG TTTGTATCTG GAGCTACCGC CGGAGTCAAA GCTGCGCATG 
CAGCACGTGG ACAAGACCCA TTGCGACTTC CAGGGCGGAC ATCACGTCAA GCTGAGCCAC 
AGCATTAGCA CGGACTCATT GCTATTTTCG GAGAACAAGG ACGAGGAGTG TAACGATGTG 
GAGGGCTGCT TCGAGATGCA GTGCCATCAG GTATTCATCG GCATGGTGAC AATGCAGTAC 
CAGGCGCAAA ACGATATCGT ACGACTCGTT GAGCATCTCG AACAGGCCTG CATACGCTTT 
GTTCACTTTA GCAAAGAAAA CGAACTGCGC TCGCGTGTCT TCTCTGAGAA AATGGGTCTA 
GAGTCCGGAT GGAACTGCCA CATCTCGCTA CTGAGCGAAC CTGAGCCGGA TAGTGAAAAG 
GAGCGGATAA GTGCCAAGCC AAAGGAGTTC CCGGAAAATA TTGGTAAACC CCAAGCCACC 
ACCTCAACCA CTGCCACCAC CACTACAACA GACCATGAGA ACAACCATCA TCCCAGTGCC 
AAAGTCATGT ACATATCGCC CCCCGCACCC GCACCTGCCA CTGCATCTGT ATCCGAATCC 
ATATCTGCAG CTAAAACCAC TGAAATTGTA GGTGACGGCC CAGAAATTAG TTATCTCTTG 
GGACCGCCAA ACAATCTGGA GTCGTCAAAG ACCCTGAGCT CTTCAGCTCC TGGTGCCATT 
TCCAATCCGG ATGAGAATCC ACTTAACGCC CTCAATAGTG GGGATTCCCA TTCACCTGAT 
AGCGAGGGCA GTAAGGAGAG CTCTCAGGAT AGCTGCACAA AGAATAACCC ACCAGGCGAT 
TCTGAGTTGG AGGCGGAAAC CGAGGCGAAC AAGCACAAGC AGCGCCATTC GCTGGACTCT 
GCCATGGACG AAAACTGCCG TTCGTTGAGC ACACTCACTG AAAGCACGGA TCAGAGTGCA 
CCCATCAACT TCGATATGTC AAATAGGGCA AAGCTGCCAA GGGGCATTGA AAACATTCGG 
CCGCATTTGG AACAGGTTGA CAATGTCCCA CTGCAAGTAT CGCTTTTTAC GGACTGCTCA 
GCGGAAGCCA CGCGTCAGAT GCTGGACATC ATGCAATCCT ACGGCGAGAT TGTTGTCTGC 
CTTGGAAGTT CGGCCAGTAA TGCCAATGCG GACATTTTTC TGCAGGCCGA CTGTAGTATA 
GCCGTGGAGC CGTTGTATCC GCAAGTGTGC CAGGATGTGG ACGCGTACAC GGAAGCCAAT 
ATCCAGAACA ACAAACTGTT GTGGCAGCGG CATTCCGGCA AGTCAAAGGG AAACGATGAT 
GAGGAGCTTC TGGAGGGCGG CCTTCATGGA TGCGCCATGC AGTCGCCAGC TCACACCATC 
TCGCCACTGT ACTTAAGCCG GCTGCTCAAC TCTCTGCCCT GTTCCATTGC CGTTTGCCGC 
GACGACCCGC TTTCGCTGGT GGCCATAATT GAGCTTTCAC GTCGATTCTC TCTGGGCCTG 
TGGAACTGCA TCCAGTACTG GGCTTGCTGC GCAGGCTCTA TCTCCATTCT GAACGTGCTG 
TGCGCGTGCC TCTCGCAACC CCCGCTCGTA ACGCCGCTGC TCGGCATTTA TCTGATGTGT 
GTGCCGGTGC CGCTAATAGC CATTTCGCTA GCACACATCG ACGTGGATCC CAAAATCATG 
AATCGGGCAA CAAGCAAAAA GCAGACGGAC TATGATTCTC GCGCCTTTGC CTTTGTCATG 
TGGTGCTACG GCTGCAAGTT TATTGTACTG GTCGTGTCTC TGCTTGTTTC CTATTGGATG 
CTCTTACCAA ACACCCAATT AAATCCCGCT GAACCAGCCA CTGAGGAACC CGTGTTGGAA 
ATCGACGATG ATGACGACCC GGAGTTTTAT CAATTTCTTT ACATTGCTAG AAGCTCTGCG 
CTGCTAGGCA TAATCCTACA CTTCGTTTTC ATTTCTGTCA CCTTTGTGCA TCGAGACCAC 
GCGCTCTGGC AGCGCAATCC ATTTACCAAT CGTATTTGGG CTTTTACATG TTTGTTCTTA 
GTCCTCATCC ATGGTATTAT TTGCTTTGTG CAACTGCTGC TGGAGGATTT CTGGGTTCAG 
CTGCCCAACC AGTGGTTGGC CTTGTTAATC TTATTTGGTG GCAGCTTGCT GAGTATGCTC 
GTCAGCGAGC TGGCGAAATT CCAGGAGAAC AAGGTTAACG CGCGCTATCA GCGTCGGGCG 
CGTCTTGACT TCGGTACCAA ATTGGGCATG AATTCGCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260023.1
CDS	143..4447
Translation	MAAKRAESPEKRASPEPTLRGLTTKVALRRLHEEIEKRVQLEHERQYGAESPYRKLRAAFVKRYDSPLGWLSIGSSLLASGALLATGMLWPALCLLAILSLDLFVVVRENNLRRTEIFRKARHAISEIKLAEQHFSDDWQQSNYPHLSNPMSPCVSLQWTYRDGKIVNLPWALLVRGDYIVLRPGHISPGPCHELETNKFFAANEIYGAAPHGDPPVKPVARPPLPNLVCCLESTPYLDNLTVCLKNSLKRPPTIYNQQRYLLVTKYIQQWGFISALVITLISGLLRLHGYGLPTDEKHNWRFVLVESSAAAIVPLLPLIFPLMWISMNLWGIARLQCFLKTPQAALEKSTSKSFEEDLDTPSYDYQNMSMLRSSVLRIWMQLWRGDSELLPRSANLVQVLGSISALCCVDKKGILSWPNPTAEKVFFLHNTDEEAREDGAGSQSSSQSESDDVPVVDEDHKERGIVAEVLDLTHDQHCPFRLEFDDHEWKAHIKSLKPLGLAILLNTCSPLTHEHYAQFCGHVTAVAMLDKDLVPVTNRRCLCELAKQIGFTDHATDRFALEGQLASYRHLQPDVVRRDIRFARQLQLSSKVKVPFPHSLSVVMRENPGGYLSLFTQGTADIILDCCDDFWDGIDLRPLSAQDRKRALDFYQRSALTSYCTAFAYRPLRHGIHGALSGPQRSGGDCLYLELPPESKLRMQHVDKTHCDFQGGHHVKLSHSISTDSLLFSENKDEECNDVEGCFEMQCHQVFIGMVTMQYQAQNDIVRLVEHLEQACIRFVHFSKENELRSRVFSEKMGLESGWNCHISLLSEPEPDSEKERISAKPKEFPENIGKPQATTSTTATTTTTDHENNHHPSAKVMYISPPAPAPATASVSESISAAKTTEIVGDGPEISYLLGPPNNLESSKTLSSSAPGAISNPDENPLNALNSGDSHSPDSEGSKESSQDSCTKNNPPGDSELEAETEANKHKQRHSLDSAMDENCRSLSTLTESTDQSAPINFDMSNRAKLPRGIENIRPHLEQVDNVPLQVSLFTDCSAEATRQMLDIMQSYGEIVVCLGSSASNANADIFLQADCSIAVEPLYPQVCQDVDAYTEANIQNNKLLWQRHSGKSKGNDDEELLEGGLHGCAMQSPAHTISPLYLSRLLNSLPCSIAVCRDDPLSLVAIIELSRRFSLGLWNCIQYWACCAGSISILNVLCACLSQPPLVTPLLGIYLMCVPVPLIAISLAHIDVDPKIMNRATSKKQTDYDSRAFAFVMWCYGCKFIVLVVSLLVSYWMLLPNTQLNPAEPATEEPVLEIDDDDDPEFYQFLYIARSSALLGIILHFVFISVTFVHRDHALWQRNPFTNRIWAFTCLFLVLIHGIICFVQLLLEDFWVQLPNQWLALLILFGGSLLSMLVSELAKFQENKVNARYQRRARLDFGTKLGMNSPF

Target ORF information:

RefSeq Version	NM_001273094.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273094.1

ORF Insert Sequence:

ATGGCAGCGA AGCGGGCGGA AAGTCCGGAG AAGCGGGCTA GTCCGGAGCC CACTCTGCGT 
GGACTAACCA CAAAGGTGGC GCTGCGGCGT CTACATGAGG AGATTGAAAA ACGGGTGCAA 
CTGGAGCACG AGCGGCAATA CGGAGCGGAG AGCCCCTATC GAAAATTGCG GGCGGCCTTT 
GTGAAGCGGT ACGATAGCCC ACTGGGATGG CTTTCCATTG GAAGCAGTTT GCTGGCTAGT 
GGAGCACTGC TCGCTACAGG AATGCTATGG CCCGCCTTGT GCCTGCTGGC CATCTTATCG 
CTGGACCTCT TTGTGGTGGT CCGGGAGAAC AACCTGCGGC GCACAGAGAT CTTCCGGAAG 
GCACGACACG CGATTTCGGA AATTAAACTA GCGGAACAGC ACTTCAGTGA CGATTGGCAG 
CAATCCAACT ATCCGCACCT AAGTAATCCC ATGTCGCCCT GCGTTTCCCT GCAGTGGACC 
TACCGAGATG GAAAAATTGT CAACTTGCCG TGGGCTTTGT TGGTGCGCGG GGATTACATC 
GTTCTACGGC CGGGACACAT TTCACCCGGA CCTTGTCACG AACTGGAGAC AAATAAATTT 
TTTGCTGCTA ACGAAATATA TGGAGCTGCT CCTCACGGAG ATCCTCCAGT GAAACCTGTG 
GCACGACCTC CGCTGCCCAA TCTCGTTTGT TGTTTGGAAA GCACACCTTA CCTGGATAAC 
CTTACTGTTT GCCTCAAAAA CTCTCTGAAG CGACCGCCGA CCATCTACAA TCAGCAGCGC 
TATCTGCTGG TCACCAAGTA TATACAGCAG TGGGGCTTCA TTAGTGCCCT AGTTATCACG 
CTCATCTCGG GGTTACTGCG CCTCCATGGC TATGGCCTGC CTACCGATGA GAAGCACAAC 
TGGCGCTTTG TGCTCGTCGA ATCCTCGGCG GCGGCCATAG TTCCCCTATT GCCGCTTATC 
TTCCCGCTCA TGTGGATATC CATGAACCTG TGGGGAATAG CTCGATTGCA GTGTTTTCTA 
AAGACGCCTC AGGCTGCGTT GGAAAAGAGT ACAAGCAAAT CGTTCGAAGA GGACCTGGAC 
ACGCCCTCGT ACGACTATCA GAATATGTCC ATGCTTCGTT CCAGTGTGCT CCGCATCTGG 
ATGCAGCTGT GGCGTGGTGA CAGTGAACTC CTGCCGCGCT CAGCCAATCT GGTACAGGTG 
CTGGGGTCCA TTTCGGCGCT GTGCTGCGTG GACAAGAAAG GTATCCTGTC GTGGCCCAAT 
CCCACCGCCG AAAAGGTATT CTTCCTGCAC AACACAGACG AGGAGGCTCG CGAGGATGGA 
GCAGGCAGTC AGTCTTCTTC ACAGAGCGAG AGCGACGACG TACCGGTTGT GGATGAGGAC 
CACAAGGAGC GCGGAATAGT GGCTGAAGTG CTGGACCTCA CGCACGACCA ACACTGCCCC 
TTTCGGCTGG AGTTCGACGA TCACGAATGG AAGGCGCACA TCAAGTCCTT GAAGCCACTG 
GGTCTTGCCA TTCTGCTAAA CACCTGTTCT CCACTCACCC ACGAGCACTA CGCACAGTTC 
TGCGGTCATG TAACCGCGGT GGCTATGCTC GACAAAGATC TGGTACCTGT GACTAATCGA 
CGCTGCCTTT GCGAACTGGC CAAACAGATT GGATTCACCG ACCATGCCAC TGATCGGTTC 
GCGCTGGAGG GTCAATTGGC TAGCTATCGC CACTTGCAAC CTGATGTTGT GCGAAGGGAC 
ATTCGTTTCG CCCGGCAGCT CCAGCTGTCC TCCAAGGTGA AGGTCCCATT CCCGCACTCT 
TTGTCTGTGG TCATGCGCGA GAATCCAGGC GGCTATTTAT CACTTTTCAC CCAGGGAACA 
GCAGACATTA TACTGGACTG CTGCGATGAC TTTTGGGACG GCATTGATCT GCGACCATTG 
TCCGCACAGG ATCGCAAGCG TGCACTGGAC TTTTATCAGC GCAGCGCGCT GACGTCCTAT 
TGTACAGCGT TTGCCTATCG ACCTCTAAGG CACGGCATCC ATGGAGCCCT TAGCGGTCCT 
CAACGGAGTG GAGGTGACTG TTTGTATCTG GAGCTACCGC CGGAGTCAAA GCTGCGCATG 
CAGCACGTGG ACAAGACCCA TTGCGACTTC CAGGGCGGAC ATCACGTCAA GCTGAGCCAC 
AGCATTAGCA CGGACTCATT GCTATTTTCG GAGAACAAGG ACGAGGAGTG TAACGATGTG 
GAGGGCTGCT TCGAGATGCA GTGCCATCAG GTATTCATCG GCATGGTGAC AATGCAGTAC 
CAGGCGCAAA ACGATATCGT ACGACTCGTT GAGCATCTCG AACAGGCCTG CATACGCTTT 
GTTCACTTTA GCAAAGAAAA CGAACTGCGC TCGCGTGTCT TCTCTGAGAA AATGGGTCTA 
GAGTCCGGAT GGAACTGCCA CATCTCGCTA CTGAGCGAAC CTGAGCCGGA TAGTGAAAAG 
GAGCGGATAA GTGCCAAGCC AAAGGAGTTC CCGGAAAATA TTGGTAAACC CCAAGCCACC 
ACCTCAACCA CTGCCACCAC CACTACAACA GACCATGAGA ACAACCATCA TCCCAGTGCC 
AAAGTCATGT ACATATCGCC CCCCGCACCC GCACCTGCCA CTGCATCTGT ATCCGAATCC 
ATATCTGCAG CTAAAACCAC TGAAATTGTA GGTGACGGCC CAGAAATTAG TTATCTCTTG 
GGACCGCCAA ACAATCTGGA GTCGTCAAAG ACCCTGAGCT CTTCAGCTCC TGGTGCCATT 
TCCAATCCGG ATGAGAATCC ACTTAACGCC CTCAATAGTG GGGATTCCCA TTCACCTGAT 
AGCGAGGGCA GTAAGGAGAG CTCTCAGGAT AGCTGCACAA AGAATAACCC ACCAGGCGAT 
TCTGAGTTGG AGGCGGAAAC CGAGGCGAAC AAGCACAAGC AGCGCCATTC GCTGGACTCT 
GCCATGGACG AAAACTGCCG TTCGTTGAGC ACACTCACTG AAAGCACGGA TCAGAGTGCA 
CCCATCAACT TCGATATGTC AAATAGGGCA AAGCTGCCAA GGGGCATTGA AAACATTCGG 
CCGCATTTGG AACAGGTTGA CAATGTCCCA CTGCAAGTAT CGCTTTTTAC GGACTGCTCA 
GCGGAAGCCA CGCGTCAGAT GCTGGACATC ATGCAATCCT ACGGCGAGAT TGTTGTCTGC 
CTTGGAAGTT CGGCCAGTAA TGCCAATGCG GACATTTTTC TGCAGGCCGA CTGTAGTATA 
GCCGTGGAGC CGTTGTATCC GCAAGTGTGC CAGGATGTGG ACGCGTACAC GGAAGCCAAT 
ATCCAGAACA ACAAACTGTT GTGGCAGCGG CATTCCGGCA AGTCAAAGGG AAACGATGAT 
GAGGAGCTTC TGGAGGGCGG CCTTCATGGA TGCGCCATGC AGTCGCCAGC TCACACCATC 
TCGCCACTGT ACTTAAGCCG GCTGCTCAAC TCTCTGCCCT GTTCCATTGC CGTTTGCCGC 
GACGACCCGC TTTCGCTGGT GGCCATAATT GAGCTTTCAC GTCGATTCTC TCTGGGCCTG 
TGGAACTGCA TCCAGTACTG GGCTTGCTGC GCAGGCTCTA TCTCCATTCT GAACGTGCTG 
TGCGCGTGCC TCTCGCAACC CCCGCTCGTA ACGCCGCTGC TCGGCATTTA TCTGATGTGT 
GTGCCGGTGC CGCTAATAGC CATTTCGCTA GCACACATCG ACGTGGATCC CAAAATCATG 
AATCGGGCAA CAAGCAAAAA GCAGACGGAC TATGATTCTC GCGCCTTTGC CTTTGTCATG 
TGGTGCTACG GCTGCAAGTT TATTGTACTG GTCGTGTCTC TGCTTGTTTC CTATTGGATG 
CTCTTACCAA ACACCCAATT AAATCCCGCT GAACCAGCCA CTGAGGAACC CGTGTTGGAA 
ATCGACGATG ATGACGACCC GGAGTTTTAT CAATTTCTTT ACATTGCTAG AAGCTCTGCG 
CTGCTAGGCA TAATCCTACA CTTCGTTTTC ATTTCTGTCA CCTTTGTGCA TCGAGACCAC 
GCGCTCTGGC AGCGCAATCC ATTTACCAAT CGTATTTGGG CTTTTACATG TTTGTTCTTA 
GTCCTCATCC ATGGTATTAT TTGCTTTGTG CAACTGCTGC TGGAGGATTT CTGGGTTCAG 
CTGCCCAACC AGTGGTTGGC CTTGTTAATC TTATTTGGTG GCAGCTTGCT GAGTATGCTC 
GTCAGCGAGC TGGCGAAATT CCAGGAGAAC AAGGTTAACG CGCGCTATCA GCGTCGGGCG 
CGTCTTGACT TCGGTACCAA ATTGGGCATG AATTCGCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(2)k05819 ( NM_134985.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608829.2 Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant C, mRNA.

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CloneID	OFa13726
Clone ID Related Accession (Same CDS sequence)	NM_001273094.1 , NM_134985.4
Accession Version	NM_134985.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) k05819, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134985.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGA AGCGGGCGGA AAGTCCGGAG AAGCGGGCTA GTCCGGAGCC CACTCTGCGT 
GGACTAACCA CAAAGGTGGC GCTGCGGCGT CTACATGAGG AGATTGAAAA ACGGGTGCAA 
CTGGAGCACG AGCGGCAATA CGGAGCGGAG AGCCCCTATC GAAAATTGCG GGCGGCCTTT 
GTGAAGCGGT ACGATAGCCC ACTGGGATGG CTTTCCATTG GAAGCAGTTT GCTGGCTAGT 
GGAGCACTGC TCGCTACAGG AATGCTATGG CCCGCCTTGT GCCTGCTGGC CATCTTATCG 
CTGGACCTCT TTGTGGTGGT CCGGGAGAAC AACCTGCGGC GCACAGAGAT CTTCCGGAAG 
GCACGACACG CGATTTCGGA AATTAAACTA GCGGAACAGC ACTTCAGTGA CGATTGGCAG 
CAATCCAACT ATCCGCACCT AAGTAATCCC ATGTCGCCCT GCGTTTCCCT GCAGTGGACC 
TACCGAGATG GAAAAATTGT CAACTTGCCG TGGGCTTTGT TGGTGCGCGG GGATTACATC 
GTTCTACGGC CGGGACACAT TTCACCCGGA CCTTGTCACG AACTGGAGAC AAATAAATTT 
TTTGCTGCTA ACGAAATATA TGGAGCTGCT CCTCACGGAG ATCCTCCAGT GAAACCTGTG 
GCACGACCTC CGCTGCCCAA TCTCGTTTGT TGTTTGGAAA GCACACCTTA CCTGGATAAC 
CTTACTGTTT GCCTCAAAAA CTCTCTGAAG CGACCGCCGA CCATCTACAA TCAGCAGCGC 
TATCTGCTGG TCACCAAGTA TATACAGCAG TGGGGCTTCA TTAGTGCCCT AGTTATCACG 
CTCATCTCGG GGTTACTGCG CCTCCATGGC TATGGCCTGC CTACCGATGA GAAGCACAAC 
TGGCGCTTTG TGCTCGTCGA ATCCTCGGCG GCGGCCATAG TTCCCCTATT GCCGCTTATC 
TTCCCGCTCA TGTGGATATC CATGAACCTG TGGGGAATAG CTCGATTGCA GTGTTTTCTA 
AAGACGCCTC AGGCTGCGTT GGAAAAGAGT ACAAGCAAAT CGTTCGAAGA GGACCTGGAC 
ACGCCCTCGT ACGACTATCA GAATATGTCC ATGCTTCGTT CCAGTGTGCT CCGCATCTGG 
ATGCAGCTGT GGCGTGGTGA CAGTGAACTC CTGCCGCGCT CAGCCAATCT GGTACAGGTG 
CTGGGGTCCA TTTCGGCGCT GTGCTGCGTG GACAAGAAAG GTATCCTGTC GTGGCCCAAT 
CCCACCGCCG AAAAGGTATT CTTCCTGCAC AACACAGACG AGGAGGCTCG CGAGGATGGA 
GCAGGCAGTC AGTCTTCTTC ACAGAGCGAG AGCGACGACG TACCGGTTGT GGATGAGGAC 
CACAAGGAGC GCGGAATAGT GGCTGAAGTG CTGGACCTCA CGCACGACCA ACACTGCCCC 
TTTCGGCTGG AGTTCGACGA TCACGAATGG AAGGCGCACA TCAAGTCCTT GAAGCCACTG 
GGTCTTGCCA TTCTGCTAAA CACCTGTTCT CCACTCACCC ACGAGCACTA CGCACAGTTC 
TGCGGTCATG TAACCGCGGT GGCTATGCTC GACAAAGATC TGGTACCTGT GACTAATCGA 
CGCTGCCTTT GCGAACTGGC CAAACAGATT GGATTCACCG ACCATGCCAC TGATCGGTTC 
GCGCTGGAGG GTCAATTGGC TAGCTATCGC CACTTGCAAC CTGATGTTGT GCGAAGGGAC 
ATTCGTTTCG CCCGGCAGCT CCAGCTGTCC TCCAAGGTGA AGGTCCCATT CCCGCACTCT 
TTGTCTGTGG TCATGCGCGA GAATCCAGGC GGCTATTTAT CACTTTTCAC CCAGGGAACA 
GCAGACATTA TACTGGACTG CTGCGATGAC TTTTGGGACG GCATTGATCT GCGACCATTG 
TCCGCACAGG ATCGCAAGCG TGCACTGGAC TTTTATCAGC GCAGCGCGCT GACGTCCTAT 
TGTACAGCGT TTGCCTATCG ACCTCTAAGG CACGGCATCC ATGGAGCCCT TAGCGGTCCT 
CAACGGAGTG GAGGTGACTG TTTGTATCTG GAGCTACCGC CGGAGTCAAA GCTGCGCATG 
CAGCACGTGG ACAAGACCCA TTGCGACTTC CAGGGCGGAC ATCACGTCAA GCTGAGCCAC 
AGCATTAGCA CGGACTCATT GCTATTTTCG GAGAACAAGG ACGAGGAGTG TAACGATGTG 
GAGGGCTGCT TCGAGATGCA GTGCCATCAG GTATTCATCG GCATGGTGAC AATGCAGTAC 
CAGGCGCAAA ACGATATCGT ACGACTCGTT GAGCATCTCG AACAGGCCTG CATACGCTTT 
GTTCACTTTA GCAAAGAAAA CGAACTGCGC TCGCGTGTCT TCTCTGAGAA AATGGGTCTA 
GAGTCCGGAT GGAACTGCCA CATCTCGCTA CTGAGCGAAC CTGAGCCGGA TAGTGAAAAG 
GAGCGGATAA GTGCCAAGCC AAAGGAGTTC CCGGAAAATA TTGGTAAACC CCAAGCCACC 
ACCTCAACCA CTGCCACCAC CACTACAACA GACCATGAGA ACAACCATCA TCCCAGTGCC 
AAAGTCATGT ACATATCGCC CCCCGCACCC GCACCTGCCA CTGCATCTGT ATCCGAATCC 
ATATCTGCAG CTAAAACCAC TGAAATTGTA GGTGACGGCC CAGAAATTAG TTATCTCTTG 
GGACCGCCAA ACAATCTGGA GTCGTCAAAG ACCCTGAGCT CTTCAGCTCC TGGTGCCATT 
TCCAATCCGG ATGAGAATCC ACTTAACGCC CTCAATAGTG GGGATTCCCA TTCACCTGAT 
AGCGAGGGCA GTAAGGAGAG CTCTCAGGAT AGCTGCACAA AGAATAACCC ACCAGGCGAT 
TCTGAGTTGG AGGCGGAAAC CGAGGCGAAC AAGCACAAGC AGCGCCATTC GCTGGACTCT 
GCCATGGACG AAAACTGCCG TTCGTTGAGC ACACTCACTG AAAGCACGGA TCAGAGTGCA 
CCCATCAACT TCGATATGTC AAATAGGGCA AAGCTGCCAA GGGGCATTGA AAACATTCGG 
CCGCATTTGG AACAGGTTGA CAATGTCCCA CTGCAAGTAT CGCTTTTTAC GGACTGCTCA 
GCGGAAGCCA CGCGTCAGAT GCTGGACATC ATGCAATCCT ACGGCGAGAT TGTTGTCTGC 
CTTGGAAGTT CGGCCAGTAA TGCCAATGCG GACATTTTTC TGCAGGCCGA CTGTAGTATA 
GCCGTGGAGC CGTTGTATCC GCAAGTGTGC CAGGATGTGG ACGCGTACAC GGAAGCCAAT 
ATCCAGAACA ACAAACTGTT GTGGCAGCGG CATTCCGGCA AGTCAAAGGG AAACGATGAT 
GAGGAGCTTC TGGAGGGCGG CCTTCATGGA TGCGCCATGC AGTCGCCAGC TCACACCATC 
TCGCCACTGT ACTTAAGCCG GCTGCTCAAC TCTCTGCCCT GTTCCATTGC CGTTTGCCGC 
GACGACCCGC TTTCGCTGGT GGCCATAATT GAGCTTTCAC GTCGATTCTC TCTGGGCCTG 
TGGAACTGCA TCCAGTACTG GGCTTGCTGC GCAGGCTCTA TCTCCATTCT GAACGTGCTG 
TGCGCGTGCC TCTCGCAACC CCCGCTCGTA ACGCCGCTGC TCGGCATTTA TCTGATGTGT 
GTGCCGGTGC CGCTAATAGC CATTTCGCTA GCACACATCG ACGTGGATCC CAAAATCATG 
AATCGGGCAA CAAGCAAAAA GCAGACGGAC TATGATTCTC GCGCCTTTGC CTTTGTCATG 
TGGTGCTACG GCTGCAAGTT TATTGTACTG GTCGTGTCTC TGCTTGTTTC CTATTGGATG 
CTCTTACCAA ACACCCAATT AAATCCCGCT GAACCAGCCA CTGAGGAACC CGTGTTGGAA 
ATCGACGATG ATGACGACCC GGAGTTTTAT CAATTTCTTT ACATTGCTAG AAGCTCTGCG 
CTGCTAGGCA TAATCCTACA CTTCGTTTTC ATTTCTGTCA CCTTTGTGCA TCGAGACCAC 
GCGCTCTGGC AGCGCAATCC ATTTACCAAT CGTATTTGGG CTTTTACATG TTTGTTCTTA 
GTCCTCATCC ATGGTATTAT TTGCTTTGTG CAACTGCTGC TGGAGGATTT CTGGGTTCAG 
CTGCCCAACC AGTGGTTGGC CTTGTTAATC TTATTTGGTG GCAGCTTGCT GAGTATGCTC 
GTCAGCGAGC TGGCGAAATT CCAGGAGAAC AAGGTTAACG CGCGCTATCA GCGTCGGGCG 
CGTCTTGACT TCGGTACCAA ATTGGGCATG AATTCGCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608829.2
CDS	143..4447
Translation	MAAKRAESPEKRASPEPTLRGLTTKVALRRLHEEIEKRVQLEHERQYGAESPYRKLRAAFVKRYDSPLGWLSIGSSLLASGALLATGMLWPALCLLAILSLDLFVVVRENNLRRTEIFRKARHAISEIKLAEQHFSDDWQQSNYPHLSNPMSPCVSLQWTYRDGKIVNLPWALLVRGDYIVLRPGHISPGPCHELETNKFFAANEIYGAAPHGDPPVKPVARPPLPNLVCCLESTPYLDNLTVCLKNSLKRPPTIYNQQRYLLVTKYIQQWGFISALVITLISGLLRLHGYGLPTDEKHNWRFVLVESSAAAIVPLLPLIFPLMWISMNLWGIARLQCFLKTPQAALEKSTSKSFEEDLDTPSYDYQNMSMLRSSVLRIWMQLWRGDSELLPRSANLVQVLGSISALCCVDKKGILSWPNPTAEKVFFLHNTDEEAREDGAGSQSSSQSESDDVPVVDEDHKERGIVAEVLDLTHDQHCPFRLEFDDHEWKAHIKSLKPLGLAILLNTCSPLTHEHYAQFCGHVTAVAMLDKDLVPVTNRRCLCELAKQIGFTDHATDRFALEGQLASYRHLQPDVVRRDIRFARQLQLSSKVKVPFPHSLSVVMRENPGGYLSLFTQGTADIILDCCDDFWDGIDLRPLSAQDRKRALDFYQRSALTSYCTAFAYRPLRHGIHGALSGPQRSGGDCLYLELPPESKLRMQHVDKTHCDFQGGHHVKLSHSISTDSLLFSENKDEECNDVEGCFEMQCHQVFIGMVTMQYQAQNDIVRLVEHLEQACIRFVHFSKENELRSRVFSEKMGLESGWNCHISLLSEPEPDSEKERISAKPKEFPENIGKPQATTSTTATTTTTDHENNHHPSAKVMYISPPAPAPATASVSESISAAKTTEIVGDGPEISYLLGPPNNLESSKTLSSSAPGAISNPDENPLNALNSGDSHSPDSEGSKESSQDSCTKNNPPGDSELEAETEANKHKQRHSLDSAMDENCRSLSTLTESTDQSAPINFDMSNRAKLPRGIENIRPHLEQVDNVPLQVSLFTDCSAEATRQMLDIMQSYGEIVVCLGSSASNANADIFLQADCSIAVEPLYPQVCQDVDAYTEANIQNNKLLWQRHSGKSKGNDDEELLEGGLHGCAMQSPAHTISPLYLSRLLNSLPCSIAVCRDDPLSLVAIIELSRRFSLGLWNCIQYWACCAGSISILNVLCACLSQPPLVTPLLGIYLMCVPVPLIAISLAHIDVDPKIMNRATSKKQTDYDSRAFAFVMWCYGCKFIVLVVSLLVSYWMLLPNTQLNPAEPATEEPVLEIDDDDDPEFYQFLYIARSSALLGIILHFVFISVTFVHRDHALWQRNPFTNRIWAFTCLFLVLIHGIICFVQLLLEDFWVQLPNQWLALLILFGGSLLSMLVSELAKFQENKVNARYQRRARLDFGTKLGMNSPF

Target ORF information:

RefSeq Version	NM_134985.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) k05819 (l(2)k05819), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134985.4

ORF Insert Sequence:

ATGGCAGCGA AGCGGGCGGA AAGTCCGGAG AAGCGGGCTA GTCCGGAGCC CACTCTGCGT 
GGACTAACCA CAAAGGTGGC GCTGCGGCGT CTACATGAGG AGATTGAAAA ACGGGTGCAA 
CTGGAGCACG AGCGGCAATA CGGAGCGGAG AGCCCCTATC GAAAATTGCG GGCGGCCTTT 
GTGAAGCGGT ACGATAGCCC ACTGGGATGG CTTTCCATTG GAAGCAGTTT GCTGGCTAGT 
GGAGCACTGC TCGCTACAGG AATGCTATGG CCCGCCTTGT GCCTGCTGGC CATCTTATCG 
CTGGACCTCT TTGTGGTGGT CCGGGAGAAC AACCTGCGGC GCACAGAGAT CTTCCGGAAG 
GCACGACACG CGATTTCGGA AATTAAACTA GCGGAACAGC ACTTCAGTGA CGATTGGCAG 
CAATCCAACT ATCCGCACCT AAGTAATCCC ATGTCGCCCT GCGTTTCCCT GCAGTGGACC 
TACCGAGATG GAAAAATTGT CAACTTGCCG TGGGCTTTGT TGGTGCGCGG GGATTACATC 
GTTCTACGGC CGGGACACAT TTCACCCGGA CCTTGTCACG AACTGGAGAC AAATAAATTT 
TTTGCTGCTA ACGAAATATA TGGAGCTGCT CCTCACGGAG ATCCTCCAGT GAAACCTGTG 
GCACGACCTC CGCTGCCCAA TCTCGTTTGT TGTTTGGAAA GCACACCTTA CCTGGATAAC 
CTTACTGTTT GCCTCAAAAA CTCTCTGAAG CGACCGCCGA CCATCTACAA TCAGCAGCGC 
TATCTGCTGG TCACCAAGTA TATACAGCAG TGGGGCTTCA TTAGTGCCCT AGTTATCACG 
CTCATCTCGG GGTTACTGCG CCTCCATGGC TATGGCCTGC CTACCGATGA GAAGCACAAC 
TGGCGCTTTG TGCTCGTCGA ATCCTCGGCG GCGGCCATAG TTCCCCTATT GCCGCTTATC 
TTCCCGCTCA TGTGGATATC CATGAACCTG TGGGGAATAG CTCGATTGCA GTGTTTTCTA 
AAGACGCCTC AGGCTGCGTT GGAAAAGAGT ACAAGCAAAT CGTTCGAAGA GGACCTGGAC 
ACGCCCTCGT ACGACTATCA GAATATGTCC ATGCTTCGTT CCAGTGTGCT CCGCATCTGG 
ATGCAGCTGT GGCGTGGTGA CAGTGAACTC CTGCCGCGCT CAGCCAATCT GGTACAGGTG 
CTGGGGTCCA TTTCGGCGCT GTGCTGCGTG GACAAGAAAG GTATCCTGTC GTGGCCCAAT 
CCCACCGCCG AAAAGGTATT CTTCCTGCAC AACACAGACG AGGAGGCTCG CGAGGATGGA 
GCAGGCAGTC AGTCTTCTTC ACAGAGCGAG AGCGACGACG TACCGGTTGT GGATGAGGAC 
CACAAGGAGC GCGGAATAGT GGCTGAAGTG CTGGACCTCA CGCACGACCA ACACTGCCCC 
TTTCGGCTGG AGTTCGACGA TCACGAATGG AAGGCGCACA TCAAGTCCTT GAAGCCACTG 
GGTCTTGCCA TTCTGCTAAA CACCTGTTCT CCACTCACCC ACGAGCACTA CGCACAGTTC 
TGCGGTCATG TAACCGCGGT GGCTATGCTC GACAAAGATC TGGTACCTGT GACTAATCGA 
CGCTGCCTTT GCGAACTGGC CAAACAGATT GGATTCACCG ACCATGCCAC TGATCGGTTC 
GCGCTGGAGG GTCAATTGGC TAGCTATCGC CACTTGCAAC CTGATGTTGT GCGAAGGGAC 
ATTCGTTTCG CCCGGCAGCT CCAGCTGTCC TCCAAGGTGA AGGTCCCATT CCCGCACTCT 
TTGTCTGTGG TCATGCGCGA GAATCCAGGC GGCTATTTAT CACTTTTCAC CCAGGGAACA 
GCAGACATTA TACTGGACTG CTGCGATGAC TTTTGGGACG GCATTGATCT GCGACCATTG 
TCCGCACAGG ATCGCAAGCG TGCACTGGAC TTTTATCAGC GCAGCGCGCT GACGTCCTAT 
TGTACAGCGT TTGCCTATCG ACCTCTAAGG CACGGCATCC ATGGAGCCCT TAGCGGTCCT 
CAACGGAGTG GAGGTGACTG TTTGTATCTG GAGCTACCGC CGGAGTCAAA GCTGCGCATG 
CAGCACGTGG ACAAGACCCA TTGCGACTTC CAGGGCGGAC ATCACGTCAA GCTGAGCCAC 
AGCATTAGCA CGGACTCATT GCTATTTTCG GAGAACAAGG ACGAGGAGTG TAACGATGTG 
GAGGGCTGCT TCGAGATGCA GTGCCATCAG GTATTCATCG GCATGGTGAC AATGCAGTAC 
CAGGCGCAAA ACGATATCGT ACGACTCGTT GAGCATCTCG AACAGGCCTG CATACGCTTT 
GTTCACTTTA GCAAAGAAAA CGAACTGCGC TCGCGTGTCT TCTCTGAGAA AATGGGTCTA 
GAGTCCGGAT GGAACTGCCA CATCTCGCTA CTGAGCGAAC CTGAGCCGGA TAGTGAAAAG 
GAGCGGATAA GTGCCAAGCC AAAGGAGTTC CCGGAAAATA TTGGTAAACC CCAAGCCACC 
ACCTCAACCA CTGCCACCAC CACTACAACA GACCATGAGA ACAACCATCA TCCCAGTGCC 
AAAGTCATGT ACATATCGCC CCCCGCACCC GCACCTGCCA CTGCATCTGT ATCCGAATCC 
ATATCTGCAG CTAAAACCAC TGAAATTGTA GGTGACGGCC CAGAAATTAG TTATCTCTTG 
GGACCGCCAA ACAATCTGGA GTCGTCAAAG ACCCTGAGCT CTTCAGCTCC TGGTGCCATT 
TCCAATCCGG ATGAGAATCC ACTTAACGCC CTCAATAGTG GGGATTCCCA TTCACCTGAT 
AGCGAGGGCA GTAAGGAGAG CTCTCAGGAT AGCTGCACAA AGAATAACCC ACCAGGCGAT 
TCTGAGTTGG AGGCGGAAAC CGAGGCGAAC AAGCACAAGC AGCGCCATTC GCTGGACTCT 
GCCATGGACG AAAACTGCCG TTCGTTGAGC ACACTCACTG AAAGCACGGA TCAGAGTGCA 
CCCATCAACT TCGATATGTC AAATAGGGCA AAGCTGCCAA GGGGCATTGA AAACATTCGG 
CCGCATTTGG AACAGGTTGA CAATGTCCCA CTGCAAGTAT CGCTTTTTAC GGACTGCTCA 
GCGGAAGCCA CGCGTCAGAT GCTGGACATC ATGCAATCCT ACGGCGAGAT TGTTGTCTGC 
CTTGGAAGTT CGGCCAGTAA TGCCAATGCG GACATTTTTC TGCAGGCCGA CTGTAGTATA 
GCCGTGGAGC CGTTGTATCC GCAAGTGTGC CAGGATGTGG ACGCGTACAC GGAAGCCAAT 
ATCCAGAACA ACAAACTGTT GTGGCAGCGG CATTCCGGCA AGTCAAAGGG AAACGATGAT 
GAGGAGCTTC TGGAGGGCGG CCTTCATGGA TGCGCCATGC AGTCGCCAGC TCACACCATC 
TCGCCACTGT ACTTAAGCCG GCTGCTCAAC TCTCTGCCCT GTTCCATTGC CGTTTGCCGC 
GACGACCCGC TTTCGCTGGT GGCCATAATT GAGCTTTCAC GTCGATTCTC TCTGGGCCTG 
TGGAACTGCA TCCAGTACTG GGCTTGCTGC GCAGGCTCTA TCTCCATTCT GAACGTGCTG 
TGCGCGTGCC TCTCGCAACC CCCGCTCGTA ACGCCGCTGC TCGGCATTTA TCTGATGTGT 
GTGCCGGTGC CGCTAATAGC CATTTCGCTA GCACACATCG ACGTGGATCC CAAAATCATG 
AATCGGGCAA CAAGCAAAAA GCAGACGGAC TATGATTCTC GCGCCTTTGC CTTTGTCATG 
TGGTGCTACG GCTGCAAGTT TATTGTACTG GTCGTGTCTC TGCTTGTTTC CTATTGGATG 
CTCTTACCAA ACACCCAATT AAATCCCGCT GAACCAGCCA CTGAGGAACC CGTGTTGGAA 
ATCGACGATG ATGACGACCC GGAGTTTTAT CAATTTCTTT ACATTGCTAG AAGCTCTGCG 
CTGCTAGGCA TAATCCTACA CTTCGTTTTC ATTTCTGTCA CCTTTGTGCA TCGAGACCAC 
GCGCTCTGGC AGCGCAATCC ATTTACCAAT CGTATTTGGG CTTTTACATG TTTGTTCTTA 
GTCCTCATCC ATGGTATTAT TTGCTTTGTG CAACTGCTGC TGGAGGATTT CTGGGTTCAG 
CTGCCCAACC AGTGGTTGGC CTTGTTAATC TTATTTGGTG GCAGCTTGCT GAGTATGCTC 
GTCAGCGAGC TGGCGAAATT CCAGGAGAAC AAGGTTAACG CGCGCTATCA GCGTCGGGCG 
CGTCTTGACT TCGGTACCAA ATTGGGCATG AATTCGCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(2)k05819 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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