Hel25E cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Hel25E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hel25E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13806 NM_001273171.1
Latest version!
Drosophila melanogaster helicase at 25E, transcript variant D (Hel25E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OFa16282 NM_164646.3
Latest version!
Drosophila melanogaster helicase at 25E, transcript variant A (Hel25E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa16282 NM_164648.2
Latest version!
Drosophila melanogaster helicase at 25E, transcript variant C (Hel25E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa16282 NM_164647.1
Latest version!
Drosophila melanogaster helicase at 25E, transcript variant B (Hel25E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13806
    Clone ID Related Accession (Same CDS sequence) NM_001273171.1
    Accession Version NM_001273171.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product helicase at 25E, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATGTAA ATATCAACGT GTTCATAACC AAAATGCTCG TACTTCTCCA CCTCGCATCC
    ACCGCTATCA CGACAATTAG TTATTATTTT GTTAATGCCA CGCGATATCG AACATTCGAT
    CTTGATCCCC TCCGTATAAG CCAACCACCA TCTTGCAAAT GGTTAAACGC GTACAACCAA
    ACGTCCAGCG CTCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260100.1
    CDS188..1105
    Translation

    Target ORF information:

    RefSeq Version NM_001273171.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster helicase at 25E, transcript variant D (Hel25E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATGTAA ATATCAACGT GTTCATAACC AAAATGCTCG TACTTCTCCA CCTCGCATCC
    ACCGCTATCA CGACAATTAG TTATTATTTT GTTAATGCCA CGCGATATCG AACATTCGAT
    CTTGATCCCC TCCGTATAAG CCAACCACCA TCTTGCAAAT GGTTAAACGC GTACAACCAA
    ACGTCCAGCG CTCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16282
    Clone ID Related Accession (Same CDS sequence) NM_164647.1 , NM_164648.2 , NM_164646.3
    Accession Version NM_164647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product helicase at 25E, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG
    CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC
    GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG
    TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG
    GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC
    ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC
    ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC
    AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA
    CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC
    ATTGAGGGAC GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723090.1
    CDS78..1352
    Translation

    Target ORF information:

    RefSeq Version NM_164647.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster helicase at 25E, transcript variant B (Hel25E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG
    CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC
    GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG
    TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG
    GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC
    ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC
    ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC
    AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA
    CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC
    ATTGAGGGAC GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16282
    Clone ID Related Accession (Same CDS sequence) NM_164647.1 , NM_164648.2 , NM_164646.3
    Accession Version NM_164648.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product helicase at 25E, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164648.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG
    CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC
    GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG
    TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG
    GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC
    ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC
    ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC
    AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA
    CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC
    ATTGAGGGAC GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723091.1
    CDS181..1455
    Translation

    Target ORF information:

    RefSeq Version NM_164648.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster helicase at 25E, transcript variant C (Hel25E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164648.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG
    CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC
    GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG
    TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG
    GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC
    ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC
    ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC
    AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA
    CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC
    ATTGAGGGAC GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16282
    Clone ID Related Accession (Same CDS sequence) NM_164647.1 , NM_164648.2 , NM_164646.3
    Accession Version NM_164646.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product helicase at 25E, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164646.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG
    CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC
    GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG
    TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG
    GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC
    ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC
    ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC
    AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA
    CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC
    ATTGAGGGAC GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723089.1
    CDS188..1462
    Translation

    Target ORF information:

    RefSeq Version NM_164646.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster helicase at 25E, transcript variant A (Hel25E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164646.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
    GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT
    TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC
    TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC
    ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG
    CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC
    GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC
    AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC
    GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG
    AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG
    CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA
    GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG
    CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG
    CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC
    GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG
    TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG
    GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC
    ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC
    ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC
    AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA
    CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC
    ATTGAGGGAC GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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