LManIII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LManIII
Entrez Gene ID	34203
Full Name	Lysosomal alpha-mannosidase III
Synonyms	CG9463,Dmel\CG9463
General protein information	Preferred Names Lysosomal alpha-mannosidase III Names CG9463 gene product from transcript CG9463-RB CG9463-PA CG9463-PB LManIII-PA LManIII-PB lysosomal alpha-mannosidase III
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29F1-29F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298833.1	NP_001285762.1	lysosomal alpha-mannosidase III, isoform B
NM_135406.4	NP_609250.2	lysosomal alpha-mannosidase III, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9468	NP_609253.1
Drosophila melanogaster (fruit fly)	CG9466	NP_609252.1
Drosophila melanogaster (fruit fly)	CG5322	NP_609407.1
Drosophila melanogaster (fruit fly)	CG9463	NP_609250.2
Drosophila melanogaster (fruit fly)	CG9465	NP_609251.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LManIII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LManIII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14148	NM_135406.4 Latest version!	Drosophila melanogaster lysosomal alpha-mannosidase III, transcript variant A (LManIII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14148	NM_001298833.1 Latest version!	Drosophila melanogaster lysosomal alpha-mannosidase III, transcript variant B (LManIII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LManIII ( NM_001298833.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285762.1 Drosophila melanogaster lysosomal alpha-mannosidase III, transcript variant B (LManIII), mRNA.

Price & Availability↑

CloneID	OFa14148
Clone ID Related Accession (Same CDS sequence)	NM_001298833.1 , NM_135406.4
Accession Version	NM_001298833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysosomal alpha-mannosidase III, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTCTG TAAAGAACTT GGCCATTTTG TTGGCCTTGT TTGCCTCTTA TATTGTCCAA 
TCTGAGGCTG TTTGTTATGA GTCATGTCCC GAAACAAAGT CCAATATGAT CAATATTCAC 
TTGGTGCCAC ACTCCCATGA TGACTTGGGT TGGCTAAAAA CGGTCGATCA GTACTATTAT 
GGCAATAAGC ACAACATTCA ACATGCTGGA GTTCAATATA TATTTGATAC CGTTGTTGCC 
GAGCTGAGCA AGGACTCACG TCGCCGCTTC ATCCAGGTGG AGACGGGTTT CTTCGCCAAG 
TGGTGGGAGG ATCAGTCGGA AACCAAGAAG AAACTGGTCA AAACATTGGT GAACGAGGGT 
CGTCTGGAAT TCACTGGTGG CGCGTGGAGC ATGAACGATG AAGCTGCCGT GAACTACCAG 
AGTGTAATTG ACCAGTTTGC CGTTGGCTTA AAGTTCTTGA ATGACAACTT TGGGGTCTGT 
GGTCGCCCTC GCGTCGGCTG GCAGATCGAT GCCTTTGGTC ACTCTCGGGA GCAGGCCTCG 
ATGTTTGCCC AAATGGCTTT CGATGGTCAG TTTTTTGCAC GCATGGATCA GAATGACAAG 
AACAAACGAG TGGATAACCT GGGCCTTGAA ATGGTCTGGG ATGCCAGCGA GACCCTGGAG 
GAAATGGACT TCTTCACGGG CATGCTCTAC AATCACTACT CTGCTCCACC TGGCTTTTGC 
TTTGACTCAC TCTGCCAAGA TGATCCCATT ATTGATGGAA AGAGCTACGA CAACAATGTC 
AAGTCCAGGG TAGATGATTT TCTCAACTAT GCTTCCAATT TGGCTGGCTA TTATCGCTCT 
AATCACATCA TGGTGCCAAT GGGTGATGAT TTTCAATATG AAAATGCCTA CATGAACTAC 
AAGAACATGG ACAAACTGAT CAAGTATGTC AATGAGCGTC AAGCCAGCGG CTCCAAGTAT 
AATATCTTCT ATTCAACTGC TGGATGCTAT CTGAACTCCC TGCACAAGAG CCTCCAAAGC 
TTCCCGAACA AGACACAGGA CTTCCTGCCC CATTCCCATG AGGCCAAGAG CTTTTGGACC 
GGCTTCTTCA CCTCTCGTCC CACCCAGAAG CGATTTGAAC GCGATGGCAA CCATATCCTC 
CAAGTGGCCA AGCAACTGAG TGTGCTAGCA GATCTTTCAG GCGAAGAACA ACATAAAGAC 
TTGGACTATC TGCGTCAGAT TATGGCTGTT ATGCAGCACC ACGATGCCAT CACAGGCACC 
GAGAAACAGG AAGTGTCCAA TGACTACGAT CGCCTTTTGT ACGATGCCAT TTTGGGAGGT 
GCCAACACAG CCCGCGATGC TCTTCGAGTG CTTACCAATC AGCCAAATGG AGAGTTCCAG 
AGCTGTCTAA AATTGAATAT AAGCGAGTGC GCTTTTACCA AGGAAAATGC AGATGACTTC 
ATGGTTACTC TGTACAATCC ATTGGCTCAC ACGTCTACGC AGTATGTGCG AGTTCCTGTC 
AAGGAGGACA GCTACCAGGT TACCGACGAG AAGGGTGGAT TGGTTGCTTC TGAGCTGGTC 
CCAGTTCCCT GGGAAGTCCT GTCCCTGGAA TTCCGCAACA ACGACACTCA ACATGAGCTG 
GTTTTCAAGG CATCCGTTAA TAAAATCGCA ACTTACTTTA TCAAAAAGAT CGATAAAACT 
ACAGAAGTTG ATATCACTGT GAACACTCAA ACTGAACAGT CTGTCAATGA GGAGGAATCA 
AATGTGAAAG TTCCCAAACG TTTTAAGAAA GTTCACTCCT TGAAAACCGA TTTAGAAACC 
CTTGATGATC AAGAGACAAT TGTTCAAAAT TCGCTTATAA AACTAGTGAT CGACAATAAC 
ACAGGACGTC TGAAGACCGT TGAAATGAAC GGAGTTTCCG AGGATATTGA TCAGACCTTT 
GGAATGTACA AGACTGCAAG ATCCTGCCAT TACATATTCC GCCAGGATGC AGACTTGGAA 
CTTGTGGAAG ATGCTTTTGA TTTCACAGTT TACGATGGTG AATCCGTCAA GGAAGTCCAC 
CAGCACGTTA ACGAGTGGAT CTCGCAAGTC ATCCGCATCT ACGAGGGTGT CAATCGAGTG 
GAGTTTGAGT GGCTAGTGGG ACCTGTTCCA ATTGACGATG AGCTGGGCAA AGAGATCGTC 
ACCATCTTCA AAAGTGGCAT CTCTTCCGGT GGAGTCTTCT ACACCGACTC CAATGGTCGT 
GAAATGATGA GAAGAGAAAA GGACAAGCGG GAGGACTTTA GTCCCGATTT AAGTGAGCAG 
CCTGTGAGCG GAAACTACTA TCCAGTCACG TCGAGGATGG CTCTACAGGA CAGTAGCAAG 
CGAATGGTCC TGCTCAATGA CCGTTCTCAG GGAGGCGCCA GCTTGGAGGA CGGACGTTTG 
GAGATGATGC TGCATCGTCG TCACATTTTC GCCGACGGCT CAGGAGCTGC AGAAGCCATC 
AATGAACAAC AGTTCGGAAA GGGACTGATT GCCCGTGGAA AACTGTTTCT TTACCTCAAC 
GCTATCGAAG ATGGAGCCAC TGCATCCGAA CGGGTGGCCG AGAAAGAGAT TCATTTGCCC 
TTCTGGAAGT TCTTCAGCAA ATCTAATAAC ATCCAAAGTG ATGTAACTAA GACGTTGTCC 
GATTTTAACG ACTTACCCCA GTCCGTTCAT CTGCTTACTC TAGAGCCCTA TTCCAAGGAC 
GAAATCTTGT TGCGTTTGGA GAACTTTTTG GATCAGACTG AGGGCAATGT GGTCAGTTTC 
AGTATTCGGC AAATTTTCGA TGATCTTGGT GGACTGGAGA TTCGTGAAAC GACCTTGGAT 
GGCAACCTTC CACTAAGCGA TATGAAGCGT CTAAAGTTCC ACCATGATGG TTCTGGTCCT 
AGTCACTCAG TTCCGGAGTA CTTTACCAGT CTGCATAAGC CTTTGGCAGC TGATAAAACT 
CAGGATGCCT CGGAGTTCAG TGTTACCTTA AAGCCCATGC AAATCCGTAC TTTCATCATT 
AAAAAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285762.1
CDS	42..3053
Translation	MSSVKNLAILLALFASYIVQSEAVCYESCPETKSNMINIHLVPHSHDDLGWLKTVDQYYYGNKHNIQHAGVQYIFDTVVAELSKDSRRRFIQVETGFFAKWWEDQSETKKKLVKTLVNEGRLEFTGGAWSMNDEAAVNYQSVIDQFAVGLKFLNDNFGVCGRPRVGWQIDAFGHSREQASMFAQMAFDGQFFARMDQNDKNKRVDNLGLEMVWDASETLEEMDFFTGMLYNHYSAPPGFCFDSLCQDDPIIDGKSYDNNVKSRVDDFLNYASNLAGYYRSNHIMVPMGDDFQYENAYMNYKNMDKLIKYVNERQASGSKYNIFYSTAGCYLNSLHKSLQSFPNKTQDFLPHSHEAKSFWTGFFTSRPTQKRFERDGNHILQVAKQLSVLADLSGEEQHKDLDYLRQIMAVMQHHDAITGTEKQEVSNDYDRLLYDAILGGANTARDALRVLTNQPNGEFQSCLKLNISECAFTKENADDFMVTLYNPLAHTSTQYVRVPVKEDSYQVTDEKGGLVASELVPVPWEVLSLEFRNNDTQHELVFKASVNKIATYFIKKIDKTTEVDITVNTQTEQSVNEEESNVKVPKRFKKVHSLKTDLETLDDQETIVQNSLIKLVIDNNTGRLKTVEMNGVSEDIDQTFGMYKTARSCHYIFRQDADLELVEDAFDFTVYDGESVKEVHQHVNEWISQVIRIYEGVNRVEFEWLVGPVPIDDELGKEIVTIFKSGISSGGVFYTDSNGREMMRREKDKREDFSPDLSEQPVSGNYYPVTSRMALQDSSKRMVLLNDRSQGGASLEDGRLEMMLHRRHIFADGSGAAEAINEQQFGKGLIARGKLFLYLNAIEDGATASERVAEKEIHLPFWKFFSKSNNIQSDVTKTLSDFNDLPQSVHLLTLEPYSKDEILLRLENFLDQTEGNVVSFSIRQIFDDLGGLEIRETTLDGNLPLSDMKRLKFHHDGSGPSHSVPEYFTSLHKPLAADKTQDASEFSVTLKPMQIRTFIIKKE

Target ORF information:

RefSeq Version	NM_001298833.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysosomal alpha-mannosidase III, transcript variant B (LManIII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298833.1

ORF Insert Sequence:

ATGAGTTCTG TAAAGAACTT GGCCATTTTG TTGGCCTTGT TTGCCTCTTA TATTGTCCAA 
TCTGAGGCTG TTTGTTATGA GTCATGTCCC GAAACAAAGT CCAATATGAT CAATATTCAC 
TTGGTGCCAC ACTCCCATGA TGACTTGGGT TGGCTAAAAA CGGTCGATCA GTACTATTAT 
GGCAATAAGC ACAACATTCA ACATGCTGGA GTTCAATATA TATTTGATAC CGTTGTTGCC 
GAGCTGAGCA AGGACTCACG TCGCCGCTTC ATCCAGGTGG AGACGGGTTT CTTCGCCAAG 
TGGTGGGAGG ATCAGTCGGA AACCAAGAAG AAACTGGTCA AAACATTGGT GAACGAGGGT 
CGTCTGGAAT TCACTGGTGG CGCGTGGAGC ATGAACGATG AAGCTGCCGT GAACTACCAG 
AGTGTAATTG ACCAGTTTGC CGTTGGCTTA AAGTTCTTGA ATGACAACTT TGGGGTCTGT 
GGTCGCCCTC GCGTCGGCTG GCAGATCGAT GCCTTTGGTC ACTCTCGGGA GCAGGCCTCG 
ATGTTTGCCC AAATGGCTTT CGATGGTCAG TTTTTTGCAC GCATGGATCA GAATGACAAG 
AACAAACGAG TGGATAACCT GGGCCTTGAA ATGGTCTGGG ATGCCAGCGA GACCCTGGAG 
GAAATGGACT TCTTCACGGG CATGCTCTAC AATCACTACT CTGCTCCACC TGGCTTTTGC 
TTTGACTCAC TCTGCCAAGA TGATCCCATT ATTGATGGAA AGAGCTACGA CAACAATGTC 
AAGTCCAGGG TAGATGATTT TCTCAACTAT GCTTCCAATT TGGCTGGCTA TTATCGCTCT 
AATCACATCA TGGTGCCAAT GGGTGATGAT TTTCAATATG AAAATGCCTA CATGAACTAC 
AAGAACATGG ACAAACTGAT CAAGTATGTC AATGAGCGTC AAGCCAGCGG CTCCAAGTAT 
AATATCTTCT ATTCAACTGC TGGATGCTAT CTGAACTCCC TGCACAAGAG CCTCCAAAGC 
TTCCCGAACA AGACACAGGA CTTCCTGCCC CATTCCCATG AGGCCAAGAG CTTTTGGACC 
GGCTTCTTCA CCTCTCGTCC CACCCAGAAG CGATTTGAAC GCGATGGCAA CCATATCCTC 
CAAGTGGCCA AGCAACTGAG TGTGCTAGCA GATCTTTCAG GCGAAGAACA ACATAAAGAC 
TTGGACTATC TGCGTCAGAT TATGGCTGTT ATGCAGCACC ACGATGCCAT CACAGGCACC 
GAGAAACAGG AAGTGTCCAA TGACTACGAT CGCCTTTTGT ACGATGCCAT TTTGGGAGGT 
GCCAACACAG CCCGCGATGC TCTTCGAGTG CTTACCAATC AGCCAAATGG AGAGTTCCAG 
AGCTGTCTAA AATTGAATAT AAGCGAGTGC GCTTTTACCA AGGAAAATGC AGATGACTTC 
ATGGTTACTC TGTACAATCC ATTGGCTCAC ACGTCTACGC AGTATGTGCG AGTTCCTGTC 
AAGGAGGACA GCTACCAGGT TACCGACGAG AAGGGTGGAT TGGTTGCTTC TGAGCTGGTC 
CCAGTTCCCT GGGAAGTCCT GTCCCTGGAA TTCCGCAACA ACGACACTCA ACATGAGCTG 
GTTTTCAAGG CATCCGTTAA TAAAATCGCA ACTTACTTTA TCAAAAAGAT CGATAAAACT 
ACAGAAGTTG ATATCACTGT GAACACTCAA ACTGAACAGT CTGTCAATGA GGAGGAATCA 
AATGTGAAAG TTCCCAAACG TTTTAAGAAA GTTCACTCCT TGAAAACCGA TTTAGAAACC 
CTTGATGATC AAGAGACAAT TGTTCAAAAT TCGCTTATAA AACTAGTGAT CGACAATAAC 
ACAGGACGTC TGAAGACCGT TGAAATGAAC GGAGTTTCCG AGGATATTGA TCAGACCTTT 
GGAATGTACA AGACTGCAAG ATCCTGCCAT TACATATTCC GCCAGGATGC AGACTTGGAA 
CTTGTGGAAG ATGCTTTTGA TTTCACAGTT TACGATGGTG AATCCGTCAA GGAAGTCCAC 
CAGCACGTTA ACGAGTGGAT CTCGCAAGTC ATCCGCATCT ACGAGGGTGT CAATCGAGTG 
GAGTTTGAGT GGCTAGTGGG ACCTGTTCCA ATTGACGATG AGCTGGGCAA AGAGATCGTC 
ACCATCTTCA AAAGTGGCAT CTCTTCCGGT GGAGTCTTCT ACACCGACTC CAATGGTCGT 
GAAATGATGA GAAGAGAAAA GGACAAGCGG GAGGACTTTA GTCCCGATTT AAGTGAGCAG 
CCTGTGAGCG GAAACTACTA TCCAGTCACG TCGAGGATGG CTCTACAGGA CAGTAGCAAG 
CGAATGGTCC TGCTCAATGA CCGTTCTCAG GGAGGCGCCA GCTTGGAGGA CGGACGTTTG 
GAGATGATGC TGCATCGTCG TCACATTTTC GCCGACGGCT CAGGAGCTGC AGAAGCCATC 
AATGAACAAC AGTTCGGAAA GGGACTGATT GCCCGTGGAA AACTGTTTCT TTACCTCAAC 
GCTATCGAAG ATGGAGCCAC TGCATCCGAA CGGGTGGCCG AGAAAGAGAT TCATTTGCCC 
TTCTGGAAGT TCTTCAGCAA ATCTAATAAC ATCCAAAGTG ATGTAACTAA GACGTTGTCC 
GATTTTAACG ACTTACCCCA GTCCGTTCAT CTGCTTACTC TAGAGCCCTA TTCCAAGGAC 
GAAATCTTGT TGCGTTTGGA GAACTTTTTG GATCAGACTG AGGGCAATGT GGTCAGTTTC 
AGTATTCGGC AAATTTTCGA TGATCTTGGT GGACTGGAGA TTCGTGAAAC GACCTTGGAT 
GGCAACCTTC CACTAAGCGA TATGAAGCGT CTAAAGTTCC ACCATGATGG TTCTGGTCCT 
AGTCACTCAG TTCCGGAGTA CTTTACCAGT CTGCATAAGC CTTTGGCAGC TGATAAAACT 
CAGGATGCCT CGGAGTTCAG TGTTACCTTA AAGCCCATGC AAATCCGTAC TTTCATCATT 
AAAAAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LManIII ( NM_135406.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609250.2 Drosophila melanogaster lysosomal alpha-mannosidase III, transcript variant A (LManIII), mRNA.

Price & Availability↑

CloneID	OFa14148
Clone ID Related Accession (Same CDS sequence)	NM_001298833.1 , NM_135406.4
Accession Version	NM_135406.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysosomal alpha-mannosidase III, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135406.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTCTG TAAAGAACTT GGCCATTTTG TTGGCCTTGT TTGCCTCTTA TATTGTCCAA 
TCTGAGGCTG TTTGTTATGA GTCATGTCCC GAAACAAAGT CCAATATGAT CAATATTCAC 
TTGGTGCCAC ACTCCCATGA TGACTTGGGT TGGCTAAAAA CGGTCGATCA GTACTATTAT 
GGCAATAAGC ACAACATTCA ACATGCTGGA GTTCAATATA TATTTGATAC CGTTGTTGCC 
GAGCTGAGCA AGGACTCACG TCGCCGCTTC ATCCAGGTGG AGACGGGTTT CTTCGCCAAG 
TGGTGGGAGG ATCAGTCGGA AACCAAGAAG AAACTGGTCA AAACATTGGT GAACGAGGGT 
CGTCTGGAAT TCACTGGTGG CGCGTGGAGC ATGAACGATG AAGCTGCCGT GAACTACCAG 
AGTGTAATTG ACCAGTTTGC CGTTGGCTTA AAGTTCTTGA ATGACAACTT TGGGGTCTGT 
GGTCGCCCTC GCGTCGGCTG GCAGATCGAT GCCTTTGGTC ACTCTCGGGA GCAGGCCTCG 
ATGTTTGCCC AAATGGCTTT CGATGGTCAG TTTTTTGCAC GCATGGATCA GAATGACAAG 
AACAAACGAG TGGATAACCT GGGCCTTGAA ATGGTCTGGG ATGCCAGCGA GACCCTGGAG 
GAAATGGACT TCTTCACGGG CATGCTCTAC AATCACTACT CTGCTCCACC TGGCTTTTGC 
TTTGACTCAC TCTGCCAAGA TGATCCCATT ATTGATGGAA AGAGCTACGA CAACAATGTC 
AAGTCCAGGG TAGATGATTT TCTCAACTAT GCTTCCAATT TGGCTGGCTA TTATCGCTCT 
AATCACATCA TGGTGCCAAT GGGTGATGAT TTTCAATATG AAAATGCCTA CATGAACTAC 
AAGAACATGG ACAAACTGAT CAAGTATGTC AATGAGCGTC AAGCCAGCGG CTCCAAGTAT 
AATATCTTCT ATTCAACTGC TGGATGCTAT CTGAACTCCC TGCACAAGAG CCTCCAAAGC 
TTCCCGAACA AGACACAGGA CTTCCTGCCC CATTCCCATG AGGCCAAGAG CTTTTGGACC 
GGCTTCTTCA CCTCTCGTCC CACCCAGAAG CGATTTGAAC GCGATGGCAA CCATATCCTC 
CAAGTGGCCA AGCAACTGAG TGTGCTAGCA GATCTTTCAG GCGAAGAACA ACATAAAGAC 
TTGGACTATC TGCGTCAGAT TATGGCTGTT ATGCAGCACC ACGATGCCAT CACAGGCACC 
GAGAAACAGG AAGTGTCCAA TGACTACGAT CGCCTTTTGT ACGATGCCAT TTTGGGAGGT 
GCCAACACAG CCCGCGATGC TCTTCGAGTG CTTACCAATC AGCCAAATGG AGAGTTCCAG 
AGCTGTCTAA AATTGAATAT AAGCGAGTGC GCTTTTACCA AGGAAAATGC AGATGACTTC 
ATGGTTACTC TGTACAATCC ATTGGCTCAC ACGTCTACGC AGTATGTGCG AGTTCCTGTC 
AAGGAGGACA GCTACCAGGT TACCGACGAG AAGGGTGGAT TGGTTGCTTC TGAGCTGGTC 
CCAGTTCCCT GGGAAGTCCT GTCCCTGGAA TTCCGCAACA ACGACACTCA ACATGAGCTG 
GTTTTCAAGG CATCCGTTAA TAAAATCGCA ACTTACTTTA TCAAAAAGAT CGATAAAACT 
ACAGAAGTTG ATATCACTGT GAACACTCAA ACTGAACAGT CTGTCAATGA GGAGGAATCA 
AATGTGAAAG TTCCCAAACG TTTTAAGAAA GTTCACTCCT TGAAAACCGA TTTAGAAACC 
CTTGATGATC AAGAGACAAT TGTTCAAAAT TCGCTTATAA AACTAGTGAT CGACAATAAC 
ACAGGACGTC TGAAGACCGT TGAAATGAAC GGAGTTTCCG AGGATATTGA TCAGACCTTT 
GGAATGTACA AGACTGCAAG ATCCTGCCAT TACATATTCC GCCAGGATGC AGACTTGGAA 
CTTGTGGAAG ATGCTTTTGA TTTCACAGTT TACGATGGTG AATCCGTCAA GGAAGTCCAC 
CAGCACGTTA ACGAGTGGAT CTCGCAAGTC ATCCGCATCT ACGAGGGTGT CAATCGAGTG 
GAGTTTGAGT GGCTAGTGGG ACCTGTTCCA ATTGACGATG AGCTGGGCAA AGAGATCGTC 
ACCATCTTCA AAAGTGGCAT CTCTTCCGGT GGAGTCTTCT ACACCGACTC CAATGGTCGT 
GAAATGATGA GAAGAGAAAA GGACAAGCGG GAGGACTTTA GTCCCGATTT AAGTGAGCAG 
CCTGTGAGCG GAAACTACTA TCCAGTCACG TCGAGGATGG CTCTACAGGA CAGTAGCAAG 
CGAATGGTCC TGCTCAATGA CCGTTCTCAG GGAGGCGCCA GCTTGGAGGA CGGACGTTTG 
GAGATGATGC TGCATCGTCG TCACATTTTC GCCGACGGCT CAGGAGCTGC AGAAGCCATC 
AATGAACAAC AGTTCGGAAA GGGACTGATT GCCCGTGGAA AACTGTTTCT TTACCTCAAC 
GCTATCGAAG ATGGAGCCAC TGCATCCGAA CGGGTGGCCG AGAAAGAGAT TCATTTGCCC 
TTCTGGAAGT TCTTCAGCAA ATCTAATAAC ATCCAAAGTG ATGTAACTAA GACGTTGTCC 
GATTTTAACG ACTTACCCCA GTCCGTTCAT CTGCTTACTC TAGAGCCCTA TTCCAAGGAC 
GAAATCTTGT TGCGTTTGGA GAACTTTTTG GATCAGACTG AGGGCAATGT GGTCAGTTTC 
AGTATTCGGC AAATTTTCGA TGATCTTGGT GGACTGGAGA TTCGTGAAAC GACCTTGGAT 
GGCAACCTTC CACTAAGCGA TATGAAGCGT CTAAAGTTCC ACCATGATGG TTCTGGTCCT 
AGTCACTCAG TTCCGGAGTA CTTTACCAGT CTGCATAAGC CTTTGGCAGC TGATAAAACT 
CAGGATGCCT CGGAGTTCAG TGTTACCTTA AAGCCCATGC AAATCCGTAC TTTCATCATT 
AAAAAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609250.2
CDS	42..3053
Translation	MSSVKNLAILLALFASYIVQSEAVCYESCPETKSNMINIHLVPHSHDDLGWLKTVDQYYYGNKHNIQHAGVQYIFDTVVAELSKDSRRRFIQVETGFFAKWWEDQSETKKKLVKTLVNEGRLEFTGGAWSMNDEAAVNYQSVIDQFAVGLKFLNDNFGVCGRPRVGWQIDAFGHSREQASMFAQMAFDGQFFARMDQNDKNKRVDNLGLEMVWDASETLEEMDFFTGMLYNHYSAPPGFCFDSLCQDDPIIDGKSYDNNVKSRVDDFLNYASNLAGYYRSNHIMVPMGDDFQYENAYMNYKNMDKLIKYVNERQASGSKYNIFYSTAGCYLNSLHKSLQSFPNKTQDFLPHSHEAKSFWTGFFTSRPTQKRFERDGNHILQVAKQLSVLADLSGEEQHKDLDYLRQIMAVMQHHDAITGTEKQEVSNDYDRLLYDAILGGANTARDALRVLTNQPNGEFQSCLKLNISECAFTKENADDFMVTLYNPLAHTSTQYVRVPVKEDSYQVTDEKGGLVASELVPVPWEVLSLEFRNNDTQHELVFKASVNKIATYFIKKIDKTTEVDITVNTQTEQSVNEEESNVKVPKRFKKVHSLKTDLETLDDQETIVQNSLIKLVIDNNTGRLKTVEMNGVSEDIDQTFGMYKTARSCHYIFRQDADLELVEDAFDFTVYDGESVKEVHQHVNEWISQVIRIYEGVNRVEFEWLVGPVPIDDELGKEIVTIFKSGISSGGVFYTDSNGREMMRREKDKREDFSPDLSEQPVSGNYYPVTSRMALQDSSKRMVLLNDRSQGGASLEDGRLEMMLHRRHIFADGSGAAEAINEQQFGKGLIARGKLFLYLNAIEDGATASERVAEKEIHLPFWKFFSKSNNIQSDVTKTLSDFNDLPQSVHLLTLEPYSKDEILLRLENFLDQTEGNVVSFSIRQIFDDLGGLEIRETTLDGNLPLSDMKRLKFHHDGSGPSHSVPEYFTSLHKPLAADKTQDASEFSVTLKPMQIRTFIIKKE

Target ORF information:

RefSeq Version	NM_135406.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysosomal alpha-mannosidase III, transcript variant A (LManIII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135406.4

ORF Insert Sequence:

ATGAGTTCTG TAAAGAACTT GGCCATTTTG TTGGCCTTGT TTGCCTCTTA TATTGTCCAA 
TCTGAGGCTG TTTGTTATGA GTCATGTCCC GAAACAAAGT CCAATATGAT CAATATTCAC 
TTGGTGCCAC ACTCCCATGA TGACTTGGGT TGGCTAAAAA CGGTCGATCA GTACTATTAT 
GGCAATAAGC ACAACATTCA ACATGCTGGA GTTCAATATA TATTTGATAC CGTTGTTGCC 
GAGCTGAGCA AGGACTCACG TCGCCGCTTC ATCCAGGTGG AGACGGGTTT CTTCGCCAAG 
TGGTGGGAGG ATCAGTCGGA AACCAAGAAG AAACTGGTCA AAACATTGGT GAACGAGGGT 
CGTCTGGAAT TCACTGGTGG CGCGTGGAGC ATGAACGATG AAGCTGCCGT GAACTACCAG 
AGTGTAATTG ACCAGTTTGC CGTTGGCTTA AAGTTCTTGA ATGACAACTT TGGGGTCTGT 
GGTCGCCCTC GCGTCGGCTG GCAGATCGAT GCCTTTGGTC ACTCTCGGGA GCAGGCCTCG 
ATGTTTGCCC AAATGGCTTT CGATGGTCAG TTTTTTGCAC GCATGGATCA GAATGACAAG 
AACAAACGAG TGGATAACCT GGGCCTTGAA ATGGTCTGGG ATGCCAGCGA GACCCTGGAG 
GAAATGGACT TCTTCACGGG CATGCTCTAC AATCACTACT CTGCTCCACC TGGCTTTTGC 
TTTGACTCAC TCTGCCAAGA TGATCCCATT ATTGATGGAA AGAGCTACGA CAACAATGTC 
AAGTCCAGGG TAGATGATTT TCTCAACTAT GCTTCCAATT TGGCTGGCTA TTATCGCTCT 
AATCACATCA TGGTGCCAAT GGGTGATGAT TTTCAATATG AAAATGCCTA CATGAACTAC 
AAGAACATGG ACAAACTGAT CAAGTATGTC AATGAGCGTC AAGCCAGCGG CTCCAAGTAT 
AATATCTTCT ATTCAACTGC TGGATGCTAT CTGAACTCCC TGCACAAGAG CCTCCAAAGC 
TTCCCGAACA AGACACAGGA CTTCCTGCCC CATTCCCATG AGGCCAAGAG CTTTTGGACC 
GGCTTCTTCA CCTCTCGTCC CACCCAGAAG CGATTTGAAC GCGATGGCAA CCATATCCTC 
CAAGTGGCCA AGCAACTGAG TGTGCTAGCA GATCTTTCAG GCGAAGAACA ACATAAAGAC 
TTGGACTATC TGCGTCAGAT TATGGCTGTT ATGCAGCACC ACGATGCCAT CACAGGCACC 
GAGAAACAGG AAGTGTCCAA TGACTACGAT CGCCTTTTGT ACGATGCCAT TTTGGGAGGT 
GCCAACACAG CCCGCGATGC TCTTCGAGTG CTTACCAATC AGCCAAATGG AGAGTTCCAG 
AGCTGTCTAA AATTGAATAT AAGCGAGTGC GCTTTTACCA AGGAAAATGC AGATGACTTC 
ATGGTTACTC TGTACAATCC ATTGGCTCAC ACGTCTACGC AGTATGTGCG AGTTCCTGTC 
AAGGAGGACA GCTACCAGGT TACCGACGAG AAGGGTGGAT TGGTTGCTTC TGAGCTGGTC 
CCAGTTCCCT GGGAAGTCCT GTCCCTGGAA TTCCGCAACA ACGACACTCA ACATGAGCTG 
GTTTTCAAGG CATCCGTTAA TAAAATCGCA ACTTACTTTA TCAAAAAGAT CGATAAAACT 
ACAGAAGTTG ATATCACTGT GAACACTCAA ACTGAACAGT CTGTCAATGA GGAGGAATCA 
AATGTGAAAG TTCCCAAACG TTTTAAGAAA GTTCACTCCT TGAAAACCGA TTTAGAAACC 
CTTGATGATC AAGAGACAAT TGTTCAAAAT TCGCTTATAA AACTAGTGAT CGACAATAAC 
ACAGGACGTC TGAAGACCGT TGAAATGAAC GGAGTTTCCG AGGATATTGA TCAGACCTTT 
GGAATGTACA AGACTGCAAG ATCCTGCCAT TACATATTCC GCCAGGATGC AGACTTGGAA 
CTTGTGGAAG ATGCTTTTGA TTTCACAGTT TACGATGGTG AATCCGTCAA GGAAGTCCAC 
CAGCACGTTA ACGAGTGGAT CTCGCAAGTC ATCCGCATCT ACGAGGGTGT CAATCGAGTG 
GAGTTTGAGT GGCTAGTGGG ACCTGTTCCA ATTGACGATG AGCTGGGCAA AGAGATCGTC 
ACCATCTTCA AAAGTGGCAT CTCTTCCGGT GGAGTCTTCT ACACCGACTC CAATGGTCGT 
GAAATGATGA GAAGAGAAAA GGACAAGCGG GAGGACTTTA GTCCCGATTT AAGTGAGCAG 
CCTGTGAGCG GAAACTACTA TCCAGTCACG TCGAGGATGG CTCTACAGGA CAGTAGCAAG 
CGAATGGTCC TGCTCAATGA CCGTTCTCAG GGAGGCGCCA GCTTGGAGGA CGGACGTTTG 
GAGATGATGC TGCATCGTCG TCACATTTTC GCCGACGGCT CAGGAGCTGC AGAAGCCATC 
AATGAACAAC AGTTCGGAAA GGGACTGATT GCCCGTGGAA AACTGTTTCT TTACCTCAAC 
GCTATCGAAG ATGGAGCCAC TGCATCCGAA CGGGTGGCCG AGAAAGAGAT TCATTTGCCC 
TTCTGGAAGT TCTTCAGCAA ATCTAATAAC ATCCAAAGTG ATGTAACTAA GACGTTGTCC 
GATTTTAACG ACTTACCCCA GTCCGTTCAT CTGCTTACTC TAGAGCCCTA TTCCAAGGAC 
GAAATCTTGT TGCGTTTGGA GAACTTTTTG GATCAGACTG AGGGCAATGT GGTCAGTTTC 
AGTATTCGGC AAATTTTCGA TGATCTTGGT GGACTGGAGA TTCGTGAAAC GACCTTGGAT 
GGCAACCTTC CACTAAGCGA TATGAAGCGT CTAAAGTTCC ACCATGATGG TTCTGGTCCT 
AGTCACTCAG TTCCGGAGTA CTTTACCAGT CTGCATAAGC CTTTGGCAGC TGATAAAACT 
CAGGATGCCT CGGAGTTCAG TGTTACCTTA AAGCCCATGC AAATCCGTAC TTTCATCATT 
AAAAAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LManIII cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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