aub cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Gene Symbol	aub
Entrez Gene ID	34524
Full Name	aubergine
Synonyms	AUB,Aub,CG6137,Dmel\CG6137,ms(2)32D,sti,sting
General protein information	Preferred Names aubergine Names CG6137 gene product from transcript CG6137-RA CG6137-PA CG6137-PC aub-PA aub-PC stellate-interacting gene
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32C1-32C1\|2-39 cM

mRNA and Protein(s)

mRNA Protein Name

NM_001103674.3 NP_001097144.1 aubergine, isoform C

NM_057386.5 NP_476734.1 aubergine, isoform A
Pathways from BioSystems

mRNA	Protein	Name
NM_001103674.3	NP_001097144.1	aubergine, isoform C
NM_057386.5	NP_476734.1	aubergine, isoform A

Homologies

Homo sapiens (human)	PIWIL1	NP_004755.2
Pan troglodytes (chimpanzee)	PIWIL1	XP_001137913.1
Xenopus tropicalis (tropical clawed frog)	LOC100496286	XP_002942570.2
Canis lupus familiaris (dog)	PIWIL1	XP_534638.2
Mus musculus (house mouse)	Piwil1	NP_067286.1
Rattus norvegicus (Norway rat)	Piwil1	NP_001102323.1
Danio rerio (zebrafish)	piwil1	NP_899181.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011204	XP_564296.2
Caenorhabditis elegans (roundworm)	prg-1	NP_492121.1
Caenorhabditis elegans (roundworm)	prg-2	NP_500994.1
Bos taurus (cattle)	PIWIL1	XP_002694505.2
Gallus gallus (chicken)	PIWIL1	NP_001092322.1
Drosophila melanogaster (fruit fly)	aub	NP_476734.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009509	XP_310187.3
Xenopus tropicalis (tropical clawed frog)	piwil1	XP_002940227.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Aubergine and piRNAs promote germline stem cell self-renewal by repressing the proto-oncogene Cbl.
Title: Aubergine and piRNAs promote germline stem cell self-renewal by repressing the proto-oncogene

Aubergine controls germline stem cell self-renewal and progeny differentiation
Title: Aubergine Controls Germline Stem Cell Self-Renewal and Progeny Differentiation via Distinct Mechanisms.

Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway.
Title: Cbp80 is needed for the expression of piRNA components and piRNAs.

The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules.
Title: RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.

We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication.
Title: Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine.

The following aub gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aub cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16680	NM_057386.5 Latest version!	Drosophila melanogaster aubergine (aub), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16685	NM_001103674.3 Latest version!	Drosophila melanogaster aubergine (aub), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aub ( NM_057386.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476734.1 Drosophila melanogaster aubergine (aub), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16680
Clone ID Related Accession (Same CDS sequence)	NM_057386.5
Accession Version	NM_057386.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aubergine, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057386.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTAC CACCAAACCC TGTAATTGCT CGTGGACGTG GTCGAGGAAG AAAGCCCAAT 
AATGTAGAGG CCAATCGCGG CTTCGCTCCT TCGCTGGGTC AGAAGTCGGA CCCTTCCCAC 
TCGGAAGGAA ATCAAGCATC CGGAGGCAAT GGTGGTGGTG GTGATGCTCA AGTGGGTCCC 
TCGATAGAGA AATCCAGTTT GAGCGCGGTG CAAATGCACA AGTCCGAGGG CGATCCGCGT 
GGCTCCGTGC GCGGCCGTAG GCTAATAACG GATTTGGTGT ATTCACGTCC TCCAGGGATG 
ACCAGCAAGA AAGGAGTGGT TGGCACTCAT ATTACCGTGC AGGCAAACTA TTTTAAGGTA 
TTAAAGCGTC CAAACTGGAC CATCTACCAG TACCGCGTCG ATTTTACGCC TGATGTGGAG 
GCTACACGAC TGCGACGGTC TTTCTTGTAT GAACATAAAG GGATCCTGGG CGGCTACATC 
TTTGACGGAA CCAATATGTT TTGCATCAAT CAGTTCAAAG CTGTTCAAGA TAGCCCCTAT 
GTTTTGGAAC TGGTTACGAA GAGTCGTGCT GGCGAAAACA TTGAAATTAA GATCAAGGCT 
GTTGGATCTG TGCAATCTAC GGATGCCGAG CAGTTTCAGG TCCTTAATCT CATACTGCGC 
AGGGCCATGG AGGGCTTAGA CTTGAAGCTG GTCTCGCGCT ACTACTACGA TCCTCAAGCT 
AAGATTAATT TGGAGAATTT CCGCATGCAA TTATGGCCTG GCTATCAGAC TTCGATTCGC 
CAGCACGAAA ATGATATATT ACTTTGTTCC GAGATATGCC ACAAGGTTAT GCGAACTGAG 
ACCTTGTACA ATATTTTGTC CGATGCTATT CGCGACAGTG ACGATTATCA AAGTACATTT 
AAGCGTGCAG TAATGGGTAT GGTAATACTA ACGGATTATA ATAACAAAAC CTATCGCATT 
GACGATGTCG ACTTTCAATC GACGCCATTG TGCAAATTTA AAACCAATGA CGGTGAGATT 
TCGTACGTGG ATTACTATAA GAAGCGATAC AACATAATCA TTCGCGACTT GAAGCAACCT 
CTGGTCATGT CTCGTCCGAC AGACAAAAAT ATTCGTGGTG GAAATGACCA GGCCATAATG 
ATCATTCCCG AGCTGGCACG GGCTACGGGA ATGACGGACG CTATGCGCGC TGACTTTAGG 
ACGTTGAGGG CCATGAGTGA ACATACCAGG CTGAATCCAG ATCGTCGCAT CGAACGCCTT 
CGCATGTTCA ACAAGCGCTT AAAGTCGTGT AAACAGAGTG TAGAGACCCT TAAGTCCTGG 
AACATCGAGC TGGACTCCGC CTTGGTGGAG ATTCCAGCAC GCGTGTTGCC ACCGGAAAAA 
ATATTATTCG GCAACCAAAA AATATTCGTA TGCGACGCTC GCGCCGATTG GACCAATGAG 
TTTCGCACCT GTTCGATGTT TAAAAACGTG CATATCAATA GGTGGTATGT GATCACACCG 
AGTCGAAATC TGCGCGAGAC CCAGGAATTT GTGCAGATGT GCATCCGCAC AGCCAGCAGC 
ATGAAGATGA ACATCTGCAA TCCAATATAT GAGGAAATTC CGGATGACCG CAACGGCACT 
TACTCCCAAG CGATCGACAA CGCCGCAGCT AACGATCCAC AGATCGTGAT GGTTGTCATG 
AGATCTCCAA ATGAAGAGAA ATATAGCTGC ATTAAAAAAC GCACGTGCGT GGACAGACCG 
GTGCCATCGC AGGTGGTGAC ACTAAAAGTT ATCGCGCCTC GACAGCAAAA ACCAACTGGG 
CTGATGTCGA TCGCCACAAA GGTGGTTATT CAAATGAACG CCAAATTGAT GGGAGCTCCC 
TGGCAGGTAG TGATCCCCCT CCACGGTCTG ATGACTGTTG GTTTCGATGT CTGCCATTCA 
CCGAAGAATA AGAACAAGTC ATATGGGGCT TTCGTTGCAA CCATGGACCA GAAGGAGTCT 
TTCCGCTACT TCTCCACCGT AAACGAACAC ATTAAGGGCC AAGAGTTGTC CGAGCAGATG 
TCGGTGAACA TGGCGTGCGC CCTGAGGTCG TATCAGGAGC AACACCGTTC TTTGCCAGAG 
CGCATTCTCT TCTTTCGCGA CGGTGTTGGC GATGGTCAGC TCTACCAGGT GGTAAACAGC 
GAGGTGAACA CCCTAAAGGA CAGGCTCGAC GAAATTTACA AATCAGCTGG CAAACAGGAG 
GGCTGTCGCA TGACATTTAT AATTGTATCC AAGCGCATTA ATTCTCGCTA CTTTACTGGG 
CATCGCAACC CAGTTCCGGG CACTGTAGTC GATGACGTTA TTACCTTGCC AGAGCGCTAC 
GACTTCTTCC TAGTGTCCCA GGCTGTTCGG ATAGGAACTG TGTCGCCTAC CAGCTACAAT 
GTTATTTCTG ACAACATGGG ACTAAACGCC GATAAGCTGC AGATGCTCTC CTATAAGATG 
ACCCATATGT ACTACAATTA CAGCGGAACC ATACGAGTCC CCGCTGTCTG CCATTACGCC 
CACAAATTGG CTTTCCTCGT AGCCGAATCC ATTAATCGTG CGCCTTCAGC AGGACTGCAG 
AATCAATTGT ACTTTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476734.1
CDS	112..2712
Translation	MNLPPNPVIARGRGRGRKPNNVEANRGFAPSLGQKSDPSHSEGNQASGGNGGGGDAQVGPSIEKSSLSAVQMHKSEGDPRGSVRGRRLITDLVYSRPPGMTSKKGVVGTHITVQANYFKVLKRPNWTIYQYRVDFTPDVEATRLRRSFLYEHKGILGGYIFDGTNMFCINQFKAVQDSPYVLELVTKSRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYYDPQAKINLENFRMQLWPGYQTSIRQHENDILLCSEICHKVMRTETLYNILSDAIRDSDDYQSTFKRAVMGMVILTDYNNKTYRIDDVDFQSTPLCKFKTNDGEISYVDYYKKRYNIIIRDLKQPLVMSRPTDKNIRGGNDQAIMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSWNIELDSALVEIPARVLPPEKILFGNQKIFVCDARADWTNEFRTCSMFKNVHINRWYVITPSRNLRETQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSCIKKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGFDVCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPERILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYFTGHRNPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADKLQMLSYKMTHMYYNYSGTIRVPAVCHYAHKLAFLVAESINRAPSAGLQNQLYFL

Target ORF information:

RefSeq Version	NM_057386.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aubergine (aub), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057386.5

ORF Insert Sequence:

ATGAATTTAC CACCAAACCC TGTAATTGCT CGTGGACGTG GTCGAGGAAG AAAGCCCAAT 
AATGTAGAGG CCAATCGCGG CTTCGCTCCT TCGCTGGGTC AGAAGTCGGA CCCTTCCCAC 
TCGGAAGGAA ATCAAGCATC CGGAGGCAAT GGTGGTGGTG GTGATGCTCA AGTGGGTCCC 
TCGATAGAGA AATCCAGTTT GAGCGCGGTG CAAATGCACA AGTCCGAGGG CGATCCGCGT 
GGCTCCGTGC GCGGCCGTAG GCTAATAACG GATTTGGTGT ATTCACGTCC TCCAGGGATG 
ACCAGCAAGA AAGGAGTGGT TGGCACTCAT ATTACCGTGC AGGCAAACTA TTTTAAGGTA 
TTAAAGCGTC CAAACTGGAC CATCTACCAG TACCGCGTCG ATTTTACGCC TGATGTGGAG 
GCTACACGAC TGCGACGGTC TTTCTTGTAT GAACATAAAG GGATCCTGGG CGGCTACATC 
TTTGACGGAA CCAATATGTT TTGCATCAAT CAGTTCAAAG CTGTTCAAGA TAGCCCCTAT 
GTTTTGGAAC TGGTTACGAA GAGTCGTGCT GGCGAAAACA TTGAAATTAA GATCAAGGCT 
GTTGGATCTG TGCAATCTAC GGATGCCGAG CAGTTTCAGG TCCTTAATCT CATACTGCGC 
AGGGCCATGG AGGGCTTAGA CTTGAAGCTG GTCTCGCGCT ACTACTACGA TCCTCAAGCT 
AAGATTAATT TGGAGAATTT CCGCATGCAA TTATGGCCTG GCTATCAGAC TTCGATTCGC 
CAGCACGAAA ATGATATATT ACTTTGTTCC GAGATATGCC ACAAGGTTAT GCGAACTGAG 
ACCTTGTACA ATATTTTGTC CGATGCTATT CGCGACAGTG ACGATTATCA AAGTACATTT 
AAGCGTGCAG TAATGGGTAT GGTAATACTA ACGGATTATA ATAACAAAAC CTATCGCATT 
GACGATGTCG ACTTTCAATC GACGCCATTG TGCAAATTTA AAACCAATGA CGGTGAGATT 
TCGTACGTGG ATTACTATAA GAAGCGATAC AACATAATCA TTCGCGACTT GAAGCAACCT 
CTGGTCATGT CTCGTCCGAC AGACAAAAAT ATTCGTGGTG GAAATGACCA GGCCATAATG 
ATCATTCCCG AGCTGGCACG GGCTACGGGA ATGACGGACG CTATGCGCGC TGACTTTAGG 
ACGTTGAGGG CCATGAGTGA ACATACCAGG CTGAATCCAG ATCGTCGCAT CGAACGCCTT 
CGCATGTTCA ACAAGCGCTT AAAGTCGTGT AAACAGAGTG TAGAGACCCT TAAGTCCTGG 
AACATCGAGC TGGACTCCGC CTTGGTGGAG ATTCCAGCAC GCGTGTTGCC ACCGGAAAAA 
ATATTATTCG GCAACCAAAA AATATTCGTA TGCGACGCTC GCGCCGATTG GACCAATGAG 
TTTCGCACCT GTTCGATGTT TAAAAACGTG CATATCAATA GGTGGTATGT GATCACACCG 
AGTCGAAATC TGCGCGAGAC CCAGGAATTT GTGCAGATGT GCATCCGCAC AGCCAGCAGC 
ATGAAGATGA ACATCTGCAA TCCAATATAT GAGGAAATTC CGGATGACCG CAACGGCACT 
TACTCCCAAG CGATCGACAA CGCCGCAGCT AACGATCCAC AGATCGTGAT GGTTGTCATG 
AGATCTCCAA ATGAAGAGAA ATATAGCTGC ATTAAAAAAC GCACGTGCGT GGACAGACCG 
GTGCCATCGC AGGTGGTGAC ACTAAAAGTT ATCGCGCCTC GACAGCAAAA ACCAACTGGG 
CTGATGTCGA TCGCCACAAA GGTGGTTATT CAAATGAACG CCAAATTGAT GGGAGCTCCC 
TGGCAGGTAG TGATCCCCCT CCACGGTCTG ATGACTGTTG GTTTCGATGT CTGCCATTCA 
CCGAAGAATA AGAACAAGTC ATATGGGGCT TTCGTTGCAA CCATGGACCA GAAGGAGTCT 
TTCCGCTACT TCTCCACCGT AAACGAACAC ATTAAGGGCC AAGAGTTGTC CGAGCAGATG 
TCGGTGAACA TGGCGTGCGC CCTGAGGTCG TATCAGGAGC AACACCGTTC TTTGCCAGAG 
CGCATTCTCT TCTTTCGCGA CGGTGTTGGC GATGGTCAGC TCTACCAGGT GGTAAACAGC 
GAGGTGAACA CCCTAAAGGA CAGGCTCGAC GAAATTTACA AATCAGCTGG CAAACAGGAG 
GGCTGTCGCA TGACATTTAT AATTGTATCC AAGCGCATTA ATTCTCGCTA CTTTACTGGG 
CATCGCAACC CAGTTCCGGG CACTGTAGTC GATGACGTTA TTACCTTGCC AGAGCGCTAC 
GACTTCTTCC TAGTGTCCCA GGCTGTTCGG ATAGGAACTG TGTCGCCTAC CAGCTACAAT 
GTTATTTCTG ACAACATGGG ACTAAACGCC GATAAGCTGC AGATGCTCTC CTATAAGATG 
ACCCATATGT ACTACAATTA CAGCGGAACC ATACGAGTCC CCGCTGTCTG CCATTACGCC 
CACAAATTGG CTTTCCTCGT AGCCGAATCC ATTAATCGTG CGCCTTCAGC AGGACTGCAG 
AATCAATTGT ACTTTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aub ( NM_001103674.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097144.1 Drosophila melanogaster aubergine (aub), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16685
Clone ID Related Accession (Same CDS sequence)	NM_001103674.3
Accession Version	NM_001103674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aubergine, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103674.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACAAGT CCGAGGGCGA TCCGCGTGGC TCCGTGCGCG GCCGTAGGCT AATAACGGAT 
TTGGTGTATT CACGTCCTCC AGGGATGACC AGCAAGAAAG GAGTGGTTGG CACTCATATT 
ACCGTGCAGG CAAACTATTT TAAGGTATTA AAGCGTCCAA ACTGGACCAT CTACCAGTAC 
CGCGTCGATT TTACGCCTGA TGTGGAGGCT ACACGACTGC GACGGTCTTT CTTGTATGAA 
CATAAAGGGA TCCTGGGCGG CTACATCTTT GACGGAACCA ATATGTTTTG CATCAATCAG 
TTCAAAGCTG TTCAAGATAG CCCCTATGTT TTGGAACTGG TTACGAAGAG TCGTGCTGGC 
GAAAACATTG AAATTAAGAT CAAGGCTGTT GGATCTGTGC AATCTACGGA TGCCGAGCAG 
TTTCAGGTCC TTAATCTCAT ACTGCGCAGG GCCATGGAGG GCTTAGACTT GAAGCTGGTC 
TCGCGCTACT ACTACGATCC TCAAGCTAAG ATTAATTTGG AGAATTTCCG CATGCAATTA 
TGGCCTGGCT ATCAGACTTC GATTCGCCAG CACGAAAATG ATATATTACT TTGTTCCGAG 
ATATGCCACA AGGTTATGCG AACTGAGACC TTGTACAATA TTTTGTCCGA TGCTATTCGC 
GACAGTGACG ATTATCAAAG TACATTTAAG CGTGCAGTAA TGGGTATGGT AATACTAACG 
GATTATAATA ACAAAACCTA TCGCATTGAC GATGTCGACT TTCAATCGAC GCCATTGTGC 
AAATTTAAAA CCAATGACGG TGAGATTTCG TACGTGGATT ACTATAAGAA GCGATACAAC 
ATAATCATTC GCGACTTGAA GCAACCTCTG GTCATGTCTC GTCCGACAGA CAAAAATATT 
CGTGGTGGAA ATGACCAGGC CATAATGATC ATTCCCGAGC TGGCACGGGC TACGGGAATG 
ACGGACGCTA TGCGCGCTGA CTTTAGGACG TTGAGGGCCA TGAGTGAACA TACCAGGCTG 
AATCCAGATC GTCGCATCGA ACGCCTTCGC ATGTTCAACA AGCGCTTAAA GTCGTGTAAA 
CAGAGTGTAG AGACCCTTAA GTCCTGGAAC ATCGAGCTGG ACTCCGCCTT GGTGGAGATT 
CCAGCACGCG TGTTGCCACC GGAAAAAATA TTATTCGGCA ACCAAAAAAT ATTCGTATGC 
GACGCTCGCG CCGATTGGAC CAATGAGTTT CGCACCTGTT CGATGTTTAA AAACGTGCAT 
ATCAATAGGT GGTATGTGAT CACACCGAGT CGAAATCTGC GCGAGACCCA GGAATTTGTG 
CAGATGTGCA TCCGCACAGC CAGCAGCATG AAGATGAACA TCTGCAATCC AATATATGAG 
GAAATTCCGG ATGACCGCAA CGGCACTTAC TCCCAAGCGA TCGACAACGC CGCAGCTAAC 
GATCCACAGA TCGTGATGGT TGTCATGAGA TCTCCAAATG AAGAGAAATA TAGCTGCATT 
AAAAAACGCA CGTGCGTGGA CAGACCGGTG CCATCGCAGG TGGTGACACT AAAAGTTATC 
GCGCCTCGAC AGCAAAAACC AACTGGGCTG ATGTCGATCG CCACAAAGGT GGTTATTCAA 
ATGAACGCCA AATTGATGGG AGCTCCCTGG CAGGTAGTGA TCCCCCTCCA CGGTCTGATG 
ACTGTTGGTT TCGATGTCTG CCATTCACCG AAGAATAAGA ACAAGTCATA TGGGGCTTTC 
GTTGCAACCA TGGACCAGAA GGAGTCTTTC CGCTACTTCT CCACCGTAAA CGAACACATT 
AAGGGCCAAG AGTTGTCCGA GCAGATGTCG GTGAACATGG CGTGCGCCCT GAGGTCGTAT 
CAGGAGCAAC ACCGTTCTTT GCCAGAGCGC ATTCTCTTCT TTCGCGACGG TGTTGGCGAT 
GGTCAGCTCT ACCAGGTGGT AAACAGCGAG GTGAACACCC TAAAGGACAG GCTCGACGAA 
ATTTACAAAT CAGCTGGCAA ACAGGAGGGC TGTCGCATGA CATTTATAAT TGTATCCAAG 
CGCATTAATT CTCGCTACTT TACTGGGCAT CGCAACCCAG TTCCGGGCAC TGTAGTCGAT 
GACGTTATTA CCTTGCCAGA GCGCTACGAC TTCTTCCTAG TGTCCCAGGC TGTTCGGATA 
GGAACTGTGT CGCCTACCAG CTACAATGTT ATTTCTGACA ACATGGGACT AAACGCCGAT 
AAGCTGCAGA TGCTCTCCTA TAAGATGACC CATATGTACT ACAATTACAG CGGAACCATA 
CGAGTCCCCG CTGTCTGCCA TTACGCCCAC AAATTGGCTT TCCTCGTAGC CGAATCCATT 
AATCGTGCGC CTTCAGCAGG ACTGCAGAAT CAATTGTACT TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097144.1
CDS	308..2695
Translation	MHKSEGDPRGSVRGRRLITDLVYSRPPGMTSKKGVVGTHITVQANYFKVLKRPNWTIYQYRVDFTPDVEATRLRRSFLYEHKGILGGYIFDGTNMFCINQFKAVQDSPYVLELVTKSRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYYDPQAKINLENFRMQLWPGYQTSIRQHENDILLCSEICHKVMRTETLYNILSDAIRDSDDYQSTFKRAVMGMVILTDYNNKTYRIDDVDFQSTPLCKFKTNDGEISYVDYYKKRYNIIIRDLKQPLVMSRPTDKNIRGGNDQAIMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSWNIELDSALVEIPARVLPPEKILFGNQKIFVCDARADWTNEFRTCSMFKNVHINRWYVITPSRNLRETQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSCIKKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGFDVCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPERILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYFTGHRNPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADKLQMLSYKMTHMYYNYSGTIRVPAVCHYAHKLAFLVAESINRAPSAGLQNQLYFL

Target ORF information:

RefSeq Version	NM_001103674.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aubergine (aub), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103674.3

ORF Insert Sequence:

ATGCACAAGT CCGAGGGCGA TCCGCGTGGC TCCGTGCGCG GCCGTAGGCT AATAACGGAT 
TTGGTGTATT CACGTCCTCC AGGGATGACC AGCAAGAAAG GAGTGGTTGG CACTCATATT 
ACCGTGCAGG CAAACTATTT TAAGGTATTA AAGCGTCCAA ACTGGACCAT CTACCAGTAC 
CGCGTCGATT TTACGCCTGA TGTGGAGGCT ACACGACTGC GACGGTCTTT CTTGTATGAA 
CATAAAGGGA TCCTGGGCGG CTACATCTTT GACGGAACCA ATATGTTTTG CATCAATCAG 
TTCAAAGCTG TTCAAGATAG CCCCTATGTT TTGGAACTGG TTACGAAGAG TCGTGCTGGC 
GAAAACATTG AAATTAAGAT CAAGGCTGTT GGATCTGTGC AATCTACGGA TGCCGAGCAG 
TTTCAGGTCC TTAATCTCAT ACTGCGCAGG GCCATGGAGG GCTTAGACTT GAAGCTGGTC 
TCGCGCTACT ACTACGATCC TCAAGCTAAG ATTAATTTGG AGAATTTCCG CATGCAATTA 
TGGCCTGGCT ATCAGACTTC GATTCGCCAG CACGAAAATG ATATATTACT TTGTTCCGAG 
ATATGCCACA AGGTTATGCG AACTGAGACC TTGTACAATA TTTTGTCCGA TGCTATTCGC 
GACAGTGACG ATTATCAAAG TACATTTAAG CGTGCAGTAA TGGGTATGGT AATACTAACG 
GATTATAATA ACAAAACCTA TCGCATTGAC GATGTCGACT TTCAATCGAC GCCATTGTGC 
AAATTTAAAA CCAATGACGG TGAGATTTCG TACGTGGATT ACTATAAGAA GCGATACAAC 
ATAATCATTC GCGACTTGAA GCAACCTCTG GTCATGTCTC GTCCGACAGA CAAAAATATT 
CGTGGTGGAA ATGACCAGGC CATAATGATC ATTCCCGAGC TGGCACGGGC TACGGGAATG 
ACGGACGCTA TGCGCGCTGA CTTTAGGACG TTGAGGGCCA TGAGTGAACA TACCAGGCTG 
AATCCAGATC GTCGCATCGA ACGCCTTCGC ATGTTCAACA AGCGCTTAAA GTCGTGTAAA 
CAGAGTGTAG AGACCCTTAA GTCCTGGAAC ATCGAGCTGG ACTCCGCCTT GGTGGAGATT 
CCAGCACGCG TGTTGCCACC GGAAAAAATA TTATTCGGCA ACCAAAAAAT ATTCGTATGC 
GACGCTCGCG CCGATTGGAC CAATGAGTTT CGCACCTGTT CGATGTTTAA AAACGTGCAT 
ATCAATAGGT GGTATGTGAT CACACCGAGT CGAAATCTGC GCGAGACCCA GGAATTTGTG 
CAGATGTGCA TCCGCACAGC CAGCAGCATG AAGATGAACA TCTGCAATCC AATATATGAG 
GAAATTCCGG ATGACCGCAA CGGCACTTAC TCCCAAGCGA TCGACAACGC CGCAGCTAAC 
GATCCACAGA TCGTGATGGT TGTCATGAGA TCTCCAAATG AAGAGAAATA TAGCTGCATT 
AAAAAACGCA CGTGCGTGGA CAGACCGGTG CCATCGCAGG TGGTGACACT AAAAGTTATC 
GCGCCTCGAC AGCAAAAACC AACTGGGCTG ATGTCGATCG CCACAAAGGT GGTTATTCAA 
ATGAACGCCA AATTGATGGG AGCTCCCTGG CAGGTAGTGA TCCCCCTCCA CGGTCTGATG 
ACTGTTGGTT TCGATGTCTG CCATTCACCG AAGAATAAGA ACAAGTCATA TGGGGCTTTC 
GTTGCAACCA TGGACCAGAA GGAGTCTTTC CGCTACTTCT CCACCGTAAA CGAACACATT 
AAGGGCCAAG AGTTGTCCGA GCAGATGTCG GTGAACATGG CGTGCGCCCT GAGGTCGTAT 
CAGGAGCAAC ACCGTTCTTT GCCAGAGCGC ATTCTCTTCT TTCGCGACGG TGTTGGCGAT 
GGTCAGCTCT ACCAGGTGGT AAACAGCGAG GTGAACACCC TAAAGGACAG GCTCGACGAA 
ATTTACAAAT CAGCTGGCAA ACAGGAGGGC TGTCGCATGA CATTTATAAT TGTATCCAAG 
CGCATTAATT CTCGCTACTT TACTGGGCAT CGCAACCCAG TTCCGGGCAC TGTAGTCGAT 
GACGTTATTA CCTTGCCAGA GCGCTACGAC TTCTTCCTAG TGTCCCAGGC TGTTCGGATA 
GGAACTGTGT CGCCTACCAG CTACAATGTT ATTTCTGACA ACATGGGACT AAACGCCGAT 
AAGCTGCAGA TGCTCTCCTA TAAGATGACC CATATGTACT ACAATTACAG CGGAACCATA 
CGAGTCCCCG CTGTCTGCCA TTACGCCCAC AAATTGGCTT TCCTCGTAGC CGAATCCATT 
AATCGTGCGC CTTCAGCAGG ACTGCAGAAT CAATTGTACT TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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aub cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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