Pkn cDNA ORF clone, Drosophila melanogaster(fruit fly)

  • Gene

  • Clones

  • gRNAs

The following Pkn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa09373 NM_176114.3
Latest version!
Drosophila melanogaster protein kinase N, transcript variant E (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa21739 NM_001299313.1
Latest version!
Drosophila melanogaster protein kinase N, transcript variant O (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OFa09369 NM_176110.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant B (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa09370 NM_176112.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant F (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa09375 NM_001201966.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant J (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa09375 NM_001259292.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant L (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa12244 NM_001169624.1
Latest version!
Drosophila melanogaster protein kinase N, transcript variant H (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa12246 NM_001259291.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant M (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa21740 NM_001299315.1
Latest version!
Drosophila melanogaster protein kinase N, transcript variant Q (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa21741 NM_001299314.1
Latest version!
Drosophila melanogaster protein kinase N, transcript variant P (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa09371 NM_176113.3
Latest version!
Drosophila melanogaster protein kinase N, transcript variant D (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa09372 NM_001103767.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant G (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa09374 NM_176111.2
Latest version!
Drosophila melanogaster protein kinase N, transcript variant C (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa12245 NM_001201967.1
Latest version!
Drosophila melanogaster protein kinase N, transcript variant K (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa12245 NM_001201968.3
Latest version!
Drosophila melanogaster protein kinase N, transcript variant S (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa21742 NM_001299312.1
Latest version!
Drosophila melanogaster protein kinase N, transcript variant N (Pkn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa09373
    Clone ID Related Accession (Same CDS sequence) NM_176114.3
    Accession Version NM_176114.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176114.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATTGTGC CAACCTTCCA TGCGCTCGGC CAAGTCTTGA GGCAGATGTT TGCGGTCCAA 
    CTCCAACAGC AGCAGCAGCG CACTCATAAT CAGCTCCATT ACCTCGATTT GTCGCCCGCG
    CGAACCATCA ACCAACTGTT AGTGTGTCCA TGCGCATCCA ATCCTGTACC TCCTGCTGCC
    CGTATTAGTC TTGTACATAT AACCCTCGAA CCGATCAATG CCAGCCGGAC GACCAGTTGC
    CTGATCGAGG AGGTAGCCGA GCCGGATTCA CAGCCGGAGA TTAAGCCGGT GGCAGAAGCG
    CAGTCTGCCA AAGTATCCGA GGCTTGTGTC GAAAGTATTC TCCCCGAGAC AGTTGAAAAG
    TTAGAAACAG CAGACCAGGT CCAGCAGGTT ATACCACAGT TGGGGAAGCT TTACGTGGGC
    AGTAGCCAGC AGCAGTATGC GCAGCAGTCA TCGCCCATCA TCCAGGAGCC AGCTACTCCT
    ACTATTTACG GAAACAGCGC CGCAGCCGGA GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG
    CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC ATCTACGCTA ACCAGTATGA GCTGAATGTG
    GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC TCACCCAGCT CCTCCACCAC CAGCAACTCC
    AATCAACAGC AGCAGCAGCA GCGCCGGAAT GTGGCCCGTG GACTGCAGTA TCGTGAATCT
    GGAGGACTCG AGACTGGACG TGCTGGCAAG CAGCCACCCA ATGCGGGCAT GTTGTCAATG
    GACAACTTCC GTTTGCTAAG CGTTCTTGGT CGCGGCCACT TTGGCAAGGT GATTCTGTCC
    CAATTGCGAA GCAACAACCA GTACTACGCT ATTAAGGCAC TGAAGAAGGG AGACATCATT
    GCCCGCGACG AAGTGGAGTC CCTGCTTAGC GAAAAGCGCA TCTTCGAGGT GGCCAACGCC
    ATGCGCCATC CGTTCTTAGT TAACTTGTAT TCGTGCTTCC AGACTGAGCA ACACGTATGC
    TTTGTGATGG AATACGCTGC TGGCGGAGAT TTGATGATGC ACATCCACAC GGACGTGTTC
    CTAGAGCCGA GAGCCGTTTT CTACGCCGCT TGTGTGGTTC TGGGCCTGCA GTACCTGCAC
    GAGAACAAGA TCATCTACCG GGACCTGAAG CTGGACAATT TGCTTTTGGA CACGGAAGGA
    TATGTGAAAA TTGCGGACTT TGGTTTGTGC AAGGAGGGCA TGGGCTTTGG TGATCGCACG
    GGCACTTTCT GTGGTACGCC CGAGTTTCTG GCACCGGAAG TGCTCACGGA AACTTCCTAC
    ACACGAGCTG TGGATTGGTG GGGCTTGGGT GTGTTGATCT TTGAGATGTT GGTTGGTGAG
    TCCCCATTCC CTGGTGACGA TGAGGAGGAA GTATTCGATT CAATTGTCAA CGATGAGGTG
    CGCTATCCGC GCTTCCTATC GCTGGAGGCC ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG
    AATCCAGAGA GACGTCTGGG ATCTTCGGAA CGGGATGCGG AGGATGTTAA GAAACAGGCA
    TTCTTCCGGT CAATTGTGTG GGATGACCTG CTCCTGCGAA AGGTTAAACC ACCATTTGTG
    CCGACAATTA ACCACTTGGA GGATGTGTCA AACTTTGACG AGGAGTTCAC GTCGGAGAAG
    GCTCAGCTTA CGCCACCGAA GGAGCCGCGA CACTTGACCG AGGAGGAGCA GTTGCTCTTC
    CAGGACTTTT CATACACGGC CGAATGGTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788294.1
    CDS91..1863
    Translation

    Target ORF information:

    RefSeq Version NM_176114.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant E (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176114.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATTGTGC CAACCTTCCA TGCGCTCGGC CAAGTCTTGA GGCAGATGTT TGCGGTCCAA 
    CTCCAACAGC AGCAGCAGCG CACTCATAAT CAGCTCCATT ACCTCGATTT GTCGCCCGCG
    CGAACCATCA ACCAACTGTT AGTGTGTCCA TGCGCATCCA ATCCTGTACC TCCTGCTGCC
    CGTATTAGTC TTGTACATAT AACCCTCGAA CCGATCAATG CCAGCCGGAC GACCAGTTGC
    CTGATCGAGG AGGTAGCCGA GCCGGATTCA CAGCCGGAGA TTAAGCCGGT GGCAGAAGCG
    CAGTCTGCCA AAGTATCCGA GGCTTGTGTC GAAAGTATTC TCCCCGAGAC AGTTGAAAAG
    TTAGAAACAG CAGACCAGGT CCAGCAGGTT ATACCACAGT TGGGGAAGCT TTACGTGGGC
    AGTAGCCAGC AGCAGTATGC GCAGCAGTCA TCGCCCATCA TCCAGGAGCC AGCTACTCCT
    ACTATTTACG GAAACAGCGC CGCAGCCGGA GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG
    CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC ATCTACGCTA ACCAGTATGA GCTGAATGTG
    GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC TCACCCAGCT CCTCCACCAC CAGCAACTCC
    AATCAACAGC AGCAGCAGCA GCGCCGGAAT GTGGCCCGTG GACTGCAGTA TCGTGAATCT
    GGAGGACTCG AGACTGGACG TGCTGGCAAG CAGCCACCCA ATGCGGGCAT GTTGTCAATG
    GACAACTTCC GTTTGCTAAG CGTTCTTGGT CGCGGCCACT TTGGCAAGGT GATTCTGTCC
    CAATTGCGAA GCAACAACCA GTACTACGCT ATTAAGGCAC TGAAGAAGGG AGACATCATT
    GCCCGCGACG AAGTGGAGTC CCTGCTTAGC GAAAAGCGCA TCTTCGAGGT GGCCAACGCC
    ATGCGCCATC CGTTCTTAGT TAACTTGTAT TCGTGCTTCC AGACTGAGCA ACACGTATGC
    TTTGTGATGG AATACGCTGC TGGCGGAGAT TTGATGATGC ACATCCACAC GGACGTGTTC
    CTAGAGCCGA GAGCCGTTTT CTACGCCGCT TGTGTGGTTC TGGGCCTGCA GTACCTGCAC
    GAGAACAAGA TCATCTACCG GGACCTGAAG CTGGACAATT TGCTTTTGGA CACGGAAGGA
    TATGTGAAAA TTGCGGACTT TGGTTTGTGC AAGGAGGGCA TGGGCTTTGG TGATCGCACG
    GGCACTTTCT GTGGTACGCC CGAGTTTCTG GCACCGGAAG TGCTCACGGA AACTTCCTAC
    ACACGAGCTG TGGATTGGTG GGGCTTGGGT GTGTTGATCT TTGAGATGTT GGTTGGTGAG
    TCCCCATTCC CTGGTGACGA TGAGGAGGAA GTATTCGATT CAATTGTCAA CGATGAGGTG
    CGCTATCCGC GCTTCCTATC GCTGGAGGCC ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG
    AATCCAGAGA GACGTCTGGG ATCTTCGGAA CGGGATGCGG AGGATGTTAA GAAACAGGCA
    TTCTTCCGGT CAATTGTGTG GGATGACCTG CTCCTGCGAA AGGTTAAACC ACCATTTGTG
    CCGACAATTA ACCACTTGGA GGATGTGTCA AACTTTGACG AGGAGTTCAC GTCGGAGAAG
    GCTCAGCTTA CGCCACCGAA GGAGCCGCGA CACTTGACCG AGGAGGAGCA GTTGCTCTTC
    CAGGACTTTT CATACACGGC CGAATGGTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21739
    Clone ID Related Accession (Same CDS sequence) NM_001299313.1
    Accession Version NM_001299313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGGTT ATACCACAGT TGGGGAAGCT TTACGTGGGC
    AGTAGCCAGC AGCAGTATGC GCAGCAGTCA TCGCCCATCA TCCAGGAGCC AGCTACTCCT
    ACTATTTACG GAAACAGCGC CGCAGCCGGA GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG
    CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC ATCTACGCTA ACCACAACTC CAATCAACAG
    CAGCAGCAGC AGCGCCGGAA TGTGGCCCGT GGACTGCAGT ATCGTGAATC TGGAGGACTC
    GAGACTGGAC GTGCTGGCAA GCAGCCACCC AATGCGGGCA TGTTGTCAAT GGACAACTTC
    CGTTTGCTAA GCGTTCTTGG TCGCGGCCAC TTTGGCAAGG TGATTCTGTC CCAATTGCGA
    AGCAACAACC AGTACTACGC TATTAAGGCA CTGAAGAAGG GAGACATCAT TGCCCGCGAC
    GAAGTGGAGT CCCTGCTTAG CGAAAAGCGC ATCTTCGAGG TGGCCAACGC CATGCGCCAT
    CCGTTCTTAG TTAACTTGTA TTCGTGCTTC CAGACTGAGC AACACGTATG CTTTGTGATG
    GAATACGCTG CTGGCGGAGA TTTGATGATG CACATCCACA CGGACGTGTT CCTAGAGCCG
    AGAGCCGTTT TCTACGCCGC TTGTGTGGTT CTGGGCCTGC AGTACCTGCA CGAGAACAAG
    ATCATCTACC GGGACCTGAA GCTGGACAAT TTGCTTTTGG ACACGGAAGG ATATGTGAAA
    ATTGCGGACT TTGGTTTGTG CAAGGAGGGC ATGGGCTTTG GTGATCGCAC GGGCACTTTC
    TGTGGTACGC CCGAGTTTCT GGCACCGGAA GTGCTCACGG AAACTTCCTA CACACGAGCT
    GTGGATTGGT GGGGCTTGGG TGTGTTGATC TTTGAGATGT TGGTTGGTGA GTCCCCATTC
    CCTGGTGACG ATGAGGAGGA AGTATTCGAT TCAATTGTCA ACGATGAGGT GCGCTATCCG
    CGCTTCCTAT CGCTGGAGGC CATAGCCGTG ATGCGTAGGC TTTTGCGCAA GAATCCAGAG
    AGACGTCTGG GATCTTCGGA ACGGGATGCG GAGGATGTTA AGAAACAGGC ATTCTTCCGG
    TCAATTGTGT GGGATGACCT GCTCCTGCGA AAGGTTAAAC CACCATTTGT GCCGACAATT
    AACCACTTGG AGGATGTGTC AAACTTTGAC GAGGAGTTCA CGTCGGAGAA GGCTCAGCTT
    ACGCCACCGA AGGAGCCGCG ACACTTGACC GAGGAGGAGC AGTTGCTCTT CCAGGACTTT
    TCATACACGG CCGAATGGTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286242.1
    CDS421..3924
    Translation

    Target ORF information:

    RefSeq Version NM_001299313.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant O (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGGTT ATACCACAGT TGGGGAAGCT TTACGTGGGC
    AGTAGCCAGC AGCAGTATGC GCAGCAGTCA TCGCCCATCA TCCAGGAGCC AGCTACTCCT
    ACTATTTACG GAAACAGCGC CGCAGCCGGA GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG
    CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC ATCTACGCTA ACCACAACTC CAATCAACAG
    CAGCAGCAGC AGCGCCGGAA TGTGGCCCGT GGACTGCAGT ATCGTGAATC TGGAGGACTC
    GAGACTGGAC GTGCTGGCAA GCAGCCACCC AATGCGGGCA TGTTGTCAAT GGACAACTTC
    CGTTTGCTAA GCGTTCTTGG TCGCGGCCAC TTTGGCAAGG TGATTCTGTC CCAATTGCGA
    AGCAACAACC AGTACTACGC TATTAAGGCA CTGAAGAAGG GAGACATCAT TGCCCGCGAC
    GAAGTGGAGT CCCTGCTTAG CGAAAAGCGC ATCTTCGAGG TGGCCAACGC CATGCGCCAT
    CCGTTCTTAG TTAACTTGTA TTCGTGCTTC CAGACTGAGC AACACGTATG CTTTGTGATG
    GAATACGCTG CTGGCGGAGA TTTGATGATG CACATCCACA CGGACGTGTT CCTAGAGCCG
    AGAGCCGTTT TCTACGCCGC TTGTGTGGTT CTGGGCCTGC AGTACCTGCA CGAGAACAAG
    ATCATCTACC GGGACCTGAA GCTGGACAAT TTGCTTTTGG ACACGGAAGG ATATGTGAAA
    ATTGCGGACT TTGGTTTGTG CAAGGAGGGC ATGGGCTTTG GTGATCGCAC GGGCACTTTC
    TGTGGTACGC CCGAGTTTCT GGCACCGGAA GTGCTCACGG AAACTTCCTA CACACGAGCT
    GTGGATTGGT GGGGCTTGGG TGTGTTGATC TTTGAGATGT TGGTTGGTGA GTCCCCATTC
    CCTGGTGACG ATGAGGAGGA AGTATTCGAT TCAATTGTCA ACGATGAGGT GCGCTATCCG
    CGCTTCCTAT CGCTGGAGGC CATAGCCGTG ATGCGTAGGC TTTTGCGCAA GAATCCAGAG
    AGACGTCTGG GATCTTCGGA ACGGGATGCG GAGGATGTTA AGAAACAGGC ATTCTTCCGG
    TCAATTGTGT GGGATGACCT GCTCCTGCGA AAGGTTAAAC CACCATTTGT GCCGACAATT
    AACCACTTGG AGGATGTGTC AAACTTTGAC GAGGAGTTCA CGTCGGAGAA GGCTCAGCTT
    ACGCCACCGA AGGAGCCGCG ACACTTGACC GAGGAGGAGC AGTTGCTCTT CCAGGACTTT
    TCATACACGG CCGAATGGTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09369
    Clone ID Related Accession (Same CDS sequence) NM_176110.2
    Accession Version NM_176110.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_176110.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGGTT ATACCACAGT TGGGGAAGCT TTACGTGGGC
    AGTAGCCAGC AGCAGTATGC GCAGCAGTCA TCGCCCATCA TCCAGGAGCC AGCTACTCCT
    ACTATTTACG GAAACAGCGC CGCAGCCGGA GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG
    CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC ATCTACGCTA ACCAGTATGA GCTGAATGTG
    GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC TCACCCAGCT CCTCCACCAC CAGCAACTCC
    AATCAACAGC AGCAGCAGCA GCGCCGGAAT GTGGCCCGTG GACTGCAGTA TCGTGAATCT
    GGAGGACTCG AGACTGGACG TGCTGGCAAG CAGCCACCCA ATGCGGGCAT GTTGTCAATG
    GACAACTTCC GTTTGCTAAG CGTTCTTGGT CGCGGCCACT TTGGCAAGGT GATTCTGTCC
    CAATTGCGAA GCAACAACCA GTACTACGCT ATTAAGGCAC TGAAGAAGGG AGACATCATT
    GCCCGCGACG AAGTGGAGTC CCTGCTTAGC GAAAAGCGCA TCTTCGAGGT GGCCAACGCC
    ATGCGCCATC CGTTCTTAGT TAACTTGTAT TCGTGCTTCC AGACTGAGCA ACACGTATGC
    TTTGTGATGG AATACGCTGC TGGCGGAGAT TTGATGATGC ACATCCACAC GGACGTGTTC
    CTAGAGCCGA GAGCCGTTTT CTACGCCGCT TGTGTGGTTC TGGGCCTGCA GTACCTGCAC
    GAGAACAAGA TCATCTACCG GGACCTGAAG CTGGACAATT TGCTTTTGGA CACGGAAGGA
    TATGTGAAAA TTGCGGACTT TGGTTTGTGC AAGGAGGGCA TGGGCTTTGG TGATCGCACG
    GGCACTTTCT GTGGTACGCC CGAGTTTCTG GCACCGGAAG TGCTCACGGA AACTTCCTAC
    ACACGAGCTG TGGATTGGTG GGGCTTGGGT GTGTTGATCT TTGAGATGTT GGTTGGTGAG
    TCCCCATTCC CTGGTGACGA TGAGGAGGAA GTATTCGATT CAATTGTCAA CGATGAGGTG
    CGCTATCCGC GCTTCCTATC GCTGGAGGCC ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG
    AATCCAGAGA GACGTCTGGG ATCTTCGGAA CGGGATGCGG AGGATGTTAA GAAACAGGCA
    TTCTTCCGGT CAATTGTGTG GGATGACCTG CTCCTGCGAA AGGTTAAACC ACCATTTGTG
    CCGACAATTA ACCACTTGGA GGATGTGTCA AACTTTGACG AGGAGTTCAC GTCGGAGAAG
    GCTCAGCTTA CGCCACCGAA GGAGCCGCGA CACTTGACCG AGGAGGAGCA GTTGCTCTTC
    CAGGACTTTT CATACACGGC CGAATGGTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788290.1
    CDS421..3993
    Translation

    Target ORF information:

    RefSeq Version NM_176110.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant B (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176110.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGGTT ATACCACAGT TGGGGAAGCT TTACGTGGGC
    AGTAGCCAGC AGCAGTATGC GCAGCAGTCA TCGCCCATCA TCCAGGAGCC AGCTACTCCT
    ACTATTTACG GAAACAGCGC CGCAGCCGGA GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG
    CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC ATCTACGCTA ACCAGTATGA GCTGAATGTG
    GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC TCACCCAGCT CCTCCACCAC CAGCAACTCC
    AATCAACAGC AGCAGCAGCA GCGCCGGAAT GTGGCCCGTG GACTGCAGTA TCGTGAATCT
    GGAGGACTCG AGACTGGACG TGCTGGCAAG CAGCCACCCA ATGCGGGCAT GTTGTCAATG
    GACAACTTCC GTTTGCTAAG CGTTCTTGGT CGCGGCCACT TTGGCAAGGT GATTCTGTCC
    CAATTGCGAA GCAACAACCA GTACTACGCT ATTAAGGCAC TGAAGAAGGG AGACATCATT
    GCCCGCGACG AAGTGGAGTC CCTGCTTAGC GAAAAGCGCA TCTTCGAGGT GGCCAACGCC
    ATGCGCCATC CGTTCTTAGT TAACTTGTAT TCGTGCTTCC AGACTGAGCA ACACGTATGC
    TTTGTGATGG AATACGCTGC TGGCGGAGAT TTGATGATGC ACATCCACAC GGACGTGTTC
    CTAGAGCCGA GAGCCGTTTT CTACGCCGCT TGTGTGGTTC TGGGCCTGCA GTACCTGCAC
    GAGAACAAGA TCATCTACCG GGACCTGAAG CTGGACAATT TGCTTTTGGA CACGGAAGGA
    TATGTGAAAA TTGCGGACTT TGGTTTGTGC AAGGAGGGCA TGGGCTTTGG TGATCGCACG
    GGCACTTTCT GTGGTACGCC CGAGTTTCTG GCACCGGAAG TGCTCACGGA AACTTCCTAC
    ACACGAGCTG TGGATTGGTG GGGCTTGGGT GTGTTGATCT TTGAGATGTT GGTTGGTGAG
    TCCCCATTCC CTGGTGACGA TGAGGAGGAA GTATTCGATT CAATTGTCAA CGATGAGGTG
    CGCTATCCGC GCTTCCTATC GCTGGAGGCC ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG
    AATCCAGAGA GACGTCTGGG ATCTTCGGAA CGGGATGCGG AGGATGTTAA GAAACAGGCA
    TTCTTCCGGT CAATTGTGTG GGATGACCTG CTCCTGCGAA AGGTTAAACC ACCATTTGTG
    CCGACAATTA ACCACTTGGA GGATGTGTCA AACTTTGACG AGGAGTTCAC GTCGGAGAAG
    GCTCAGCTTA CGCCACCGAA GGAGCCGCGA CACTTGACCG AGGAGGAGCA GTTGCTCTTC
    CAGGACTTTT CATACACGGC CGAATGGTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09370
    Clone ID Related Accession (Same CDS sequence) NM_176112.2
    Accession Version NM_176112.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_176112.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGGTTATACC ACAGTTGGGG AAGCTTTACG TGGGCAGTAG CCAGCAGCAG
    TATGCGCAGC AGTCATCGCC CATCATCCAG GAGCCAGCTA CTCCTACTAT TTACGGAAAC
    AGCGCCGCAG CCGGAGCGCC GCAGTTCCCG CAGCCCGCCC AAAGGCAAGA GAAGCAGCCT
    CCGCAGCAGC AGCCCATCTA CGCTAACCAG TATGAGCTGA ATGTGGCCAA GGCGGCAGCT
    GCAGCATCTG TTTACTCACC CAGCTCCTCC ACCACCAGCA ACTCCAATCA ACAGCAGCAG
    CAGCAGCGCC GGAATGTGGC CCGTGGACTG CAGTATCGTG AATCTGGAGG ACTCGAGACT
    GGACGTGCTG GCAAGCAGCC ACCCAATGCG GGCATGTTGT CAATGGACAA CTTCCGTTTG
    CTAAGCGTTC TTGGTCGCGG CCACTTTGGC AAGGTGATTC TGTCCCAATT GCGAAGCAAC
    AACCAGTACT ACGCTATTAA GGCACTGAAG AAGGGAGACA TCATTGCCCG CGACGAAGTG
    GAGTCCCTGC TTAGCGAAAA GCGCATCTTC GAGGTGGCCA ACGCCATGCG CCATCCGTTC
    TTAGTTAACT TGTATTCGTG CTTCCAGACT GAGCAACACG TATGCTTTGT GATGGAATAC
    GCTGCTGGCG GAGATTTGAT GATGCACATC CACACGGACG TGTTCCTAGA GCCGAGAGCC
    GTTTTCTACG CCGCTTGTGT GGTTCTGGGC CTGCAGTACC TGCACGAGAA CAAGATCATC
    TACCGGGACC TGAAGCTGGA CAATTTGCTT TTGGACACGG AAGGATATGT GAAAATTGCG
    GACTTTGGTT TGTGCAAGGA GGGCATGGGC TTTGGTGATC GCACGGGCAC TTTCTGTGGT
    ACGCCCGAGT TTCTGGCACC GGAAGTGCTC ACGGAAACTT CCTACACACG AGCTGTGGAT
    TGGTGGGGCT TGGGTGTGTT GATCTTTGAG ATGTTGGTTG GTGAGTCCCC ATTCCCTGGT
    GACGATGAGG AGGAAGTATT CGATTCAATT GTCAACGATG AGGTGCGCTA TCCGCGCTTC
    CTATCGCTGG AGGCCATAGC CGTGATGCGT AGGCTTTTGC GCAAGAATCC AGAGAGACGT
    CTGGGATCTT CGGAACGGGA TGCGGAGGAT GTTAAGAAAC AGGCATTCTT CCGGTCAATT
    GTGTGGGATG ACCTGCTCCT GCGAAAGGTT AAACCACCAT TTGTGCCGAC AATTAACCAC
    TTGGAGGATG TGTCAAACTT TGACGAGGAG TTCACGTCGG AGAAGGCTCA GCTTACGCCA
    CCGAAGGAGC CGCGACACTT GACCGAGGAG GAGCAGTTGC TCTTCCAGGA CTTTTCATAC
    ACGGCCGAAT GGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788292.1
    CDS498..4115
    Translation

    Target ORF information:

    RefSeq Version NM_176112.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant F (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176112.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGGTTATACC ACAGTTGGGG AAGCTTTACG TGGGCAGTAG CCAGCAGCAG
    TATGCGCAGC AGTCATCGCC CATCATCCAG GAGCCAGCTA CTCCTACTAT TTACGGAAAC
    AGCGCCGCAG CCGGAGCGCC GCAGTTCCCG CAGCCCGCCC AAAGGCAAGA GAAGCAGCCT
    CCGCAGCAGC AGCCCATCTA CGCTAACCAG TATGAGCTGA ATGTGGCCAA GGCGGCAGCT
    GCAGCATCTG TTTACTCACC CAGCTCCTCC ACCACCAGCA ACTCCAATCA ACAGCAGCAG
    CAGCAGCGCC GGAATGTGGC CCGTGGACTG CAGTATCGTG AATCTGGAGG ACTCGAGACT
    GGACGTGCTG GCAAGCAGCC ACCCAATGCG GGCATGTTGT CAATGGACAA CTTCCGTTTG
    CTAAGCGTTC TTGGTCGCGG CCACTTTGGC AAGGTGATTC TGTCCCAATT GCGAAGCAAC
    AACCAGTACT ACGCTATTAA GGCACTGAAG AAGGGAGACA TCATTGCCCG CGACGAAGTG
    GAGTCCCTGC TTAGCGAAAA GCGCATCTTC GAGGTGGCCA ACGCCATGCG CCATCCGTTC
    TTAGTTAACT TGTATTCGTG CTTCCAGACT GAGCAACACG TATGCTTTGT GATGGAATAC
    GCTGCTGGCG GAGATTTGAT GATGCACATC CACACGGACG TGTTCCTAGA GCCGAGAGCC
    GTTTTCTACG CCGCTTGTGT GGTTCTGGGC CTGCAGTACC TGCACGAGAA CAAGATCATC
    TACCGGGACC TGAAGCTGGA CAATTTGCTT TTGGACACGG AAGGATATGT GAAAATTGCG
    GACTTTGGTT TGTGCAAGGA GGGCATGGGC TTTGGTGATC GCACGGGCAC TTTCTGTGGT
    ACGCCCGAGT TTCTGGCACC GGAAGTGCTC ACGGAAACTT CCTACACACG AGCTGTGGAT
    TGGTGGGGCT TGGGTGTGTT GATCTTTGAG ATGTTGGTTG GTGAGTCCCC ATTCCCTGGT
    GACGATGAGG AGGAAGTATT CGATTCAATT GTCAACGATG AGGTGCGCTA TCCGCGCTTC
    CTATCGCTGG AGGCCATAGC CGTGATGCGT AGGCTTTTGC GCAAGAATCC AGAGAGACGT
    CTGGGATCTT CGGAACGGGA TGCGGAGGAT GTTAAGAAAC AGGCATTCTT CCGGTCAATT
    GTGTGGGATG ACCTGCTCCT GCGAAAGGTT AAACCACCAT TTGTGCCGAC AATTAACCAC
    TTGGAGGATG TGTCAAACTT TGACGAGGAG TTCACGTCGG AGAAGGCTCA GCTTACGCCA
    CCGAAGGAGC CGCGACACTT GACCGAGGAG GAGCAGTTGC TCTTCCAGGA CTTTTCATAC
    ACGGCCGAAT GGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09375
    Clone ID Related Accession (Same CDS sequence) NM_001201966.2 , NM_001259292.2
    Accession Version NM_001201966.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001201966.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGGCCCGTAT TAGTCTTGTA CATATAACCC TCGAACCGAT CAATGCCAGC
    CGGACGACCA GTTGCCTGAT CGAGGAGGTA GCCGAGCCGG ATTCACAGCC GGAGATTAAG
    CCGGTGGCAG AAGCGCAGTC TGCCAAAGTA TCCGAGGCTT GTGTCGAAAG TATTCTCCCC
    GAGACAGTTG AAAAGTTAGA AACAGCAGAC CAGGTCCAGC AGGTTATACC ACAGTTGGGG
    AAGCTTTACG TGGGCAGTAG CCAGCAGCAG TATGCGCAGC AGTCATCGCC CATCATCCAG
    GAGCCAGCTA CTCCTACTAT TTACGGAAAC AGCGCCGCAG CCGGAGCGCC GCAGTTCCCG
    CAGCCCGCCC AAAGGCAAGA GAAGCAGCCT CCGCAGCAGC AGCCCATCTA CGCTAACCAG
    TATGAGCTGA ATGTGGCCAA GGCGGCAGCT GCAGCATCTG TTTACTCACC CAGCTCCTCC
    ACCACCAGCA ACTCCAATCA ACAGCAGCAG CAGCAGCGCC GGAATGTGGC CCGTGGACTG
    CAGTATCGTG AATCTGGAGG ACTCGAGACT GGACGTGCTG GCAAGCAGCC ACCCAATGCG
    GGCATGTTGT CAATGGACAA CTTCCGTTTG CTAAGCGTTC TTGGTCGCGG CCACTTTGGC
    AAGGTGATTC TGTCCCAATT GCGAAGCAAC AACCAGTACT ACGCTATTAA GGCACTGAAG
    AAGGGAGACA TCATTGCCCG CGACGAAGTG GAGTCCCTGC TTAGCGAAAA GCGCATCTTC
    GAGGTGGCCA ACGCCATGCG CCATCCGTTC TTAGTTAACT TGTATTCGTG CTTCCAGACT
    GAGCAACACG TATGCTTTGT GATGGAATAC GCTGCTGGCG GAGATTTGAT GATGCACATC
    CACACGGACG TGTTCCTAGA GCCGAGAGCC GTTTTCTACG CCGCTTGTGT GGTTCTGGGC
    CTGCAGTACC TGCACGAGAA CAAGATCATC TACCGGGACC TGAAGCTGGA CAATTTGCTT
    TTGGACACGG AAGGATATGT GAAAATTGCG GACTTTGGTT TGTGCAAGGA GGGCATGGGC
    TTTGGTGATC GCACGGGCAC TTTCTGTGGT ACGCCCGAGT TTCTGGCACC GGAAGTGCTC
    ACGGAAACTT CCTACACACG AGCTGTGGAT TGGTGGGGCT TGGGTGTGTT GATCTTTGAG
    ATGTTGGTTG GTGAGTCCCC ATTCCCTGGT GACGATGAGG AGGAAGTATT CGATTCAATT
    GTCAACGATG AGGTGCGCTA TCCGCGCTTC CTATCGCTGG AGGCCATAGC CGTGATGCGT
    AGGCTTTTGC GCAAGAATCC AGAGAGACGT CTGGGATCTT CGGAACGGGA TGCGGAGGAT
    GTTAAGAAAC AGGCATTCTT CCGGTCAATT GTGTGGGATG ACCTGCTCCT GCGAAAGGTT
    AAACCACCAT TTGTGCCGAC AATTAACCAC TTGGAGGATG TGTCAAACTT TGACGAGGAG
    TTCACGTCGG AGAAGGCTCA GCTTACGCCA CCGAAGGAGC CGCGACACTT GACCGAGGAG
    GAGCAGTTGC TCTTCCAGGA CTTTTCATAC ACGGCCGAAT GGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188895.1
    CDS915..4742
    Translation

    Target ORF information:

    RefSeq Version NM_001201966.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant J (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGGCCCGTAT TAGTCTTGTA CATATAACCC TCGAACCGAT CAATGCCAGC
    CGGACGACCA GTTGCCTGAT CGAGGAGGTA GCCGAGCCGG ATTCACAGCC GGAGATTAAG
    CCGGTGGCAG AAGCGCAGTC TGCCAAAGTA TCCGAGGCTT GTGTCGAAAG TATTCTCCCC
    GAGACAGTTG AAAAGTTAGA AACAGCAGAC CAGGTCCAGC AGGTTATACC ACAGTTGGGG
    AAGCTTTACG TGGGCAGTAG CCAGCAGCAG TATGCGCAGC AGTCATCGCC CATCATCCAG
    GAGCCAGCTA CTCCTACTAT TTACGGAAAC AGCGCCGCAG CCGGAGCGCC GCAGTTCCCG
    CAGCCCGCCC AAAGGCAAGA GAAGCAGCCT CCGCAGCAGC AGCCCATCTA CGCTAACCAG
    TATGAGCTGA ATGTGGCCAA GGCGGCAGCT GCAGCATCTG TTTACTCACC CAGCTCCTCC
    ACCACCAGCA ACTCCAATCA ACAGCAGCAG CAGCAGCGCC GGAATGTGGC CCGTGGACTG
    CAGTATCGTG AATCTGGAGG ACTCGAGACT GGACGTGCTG GCAAGCAGCC ACCCAATGCG
    GGCATGTTGT CAATGGACAA CTTCCGTTTG CTAAGCGTTC TTGGTCGCGG CCACTTTGGC
    AAGGTGATTC TGTCCCAATT GCGAAGCAAC AACCAGTACT ACGCTATTAA GGCACTGAAG
    AAGGGAGACA TCATTGCCCG CGACGAAGTG GAGTCCCTGC TTAGCGAAAA GCGCATCTTC
    GAGGTGGCCA ACGCCATGCG CCATCCGTTC TTAGTTAACT TGTATTCGTG CTTCCAGACT
    GAGCAACACG TATGCTTTGT GATGGAATAC GCTGCTGGCG GAGATTTGAT GATGCACATC
    CACACGGACG TGTTCCTAGA GCCGAGAGCC GTTTTCTACG CCGCTTGTGT GGTTCTGGGC
    CTGCAGTACC TGCACGAGAA CAAGATCATC TACCGGGACC TGAAGCTGGA CAATTTGCTT
    TTGGACACGG AAGGATATGT GAAAATTGCG GACTTTGGTT TGTGCAAGGA GGGCATGGGC
    TTTGGTGATC GCACGGGCAC TTTCTGTGGT ACGCCCGAGT TTCTGGCACC GGAAGTGCTC
    ACGGAAACTT CCTACACACG AGCTGTGGAT TGGTGGGGCT TGGGTGTGTT GATCTTTGAG
    ATGTTGGTTG GTGAGTCCCC ATTCCCTGGT GACGATGAGG AGGAAGTATT CGATTCAATT
    GTCAACGATG AGGTGCGCTA TCCGCGCTTC CTATCGCTGG AGGCCATAGC CGTGATGCGT
    AGGCTTTTGC GCAAGAATCC AGAGAGACGT CTGGGATCTT CGGAACGGGA TGCGGAGGAT
    GTTAAGAAAC AGGCATTCTT CCGGTCAATT GTGTGGGATG ACCTGCTCCT GCGAAAGGTT
    AAACCACCAT TTGTGCCGAC AATTAACCAC TTGGAGGATG TGTCAAACTT TGACGAGGAG
    TTCACGTCGG AGAAGGCTCA GCTTACGCCA CCGAAGGAGC CGCGACACTT GACCGAGGAG
    GAGCAGTTGC TCTTCCAGGA CTTTTCATAC ACGGCCGAAT GGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09375
    Clone ID Related Accession (Same CDS sequence) NM_001201966.2 , NM_001259292.2
    Accession Version NM_001259292.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259292.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGGCCCGTAT TAGTCTTGTA CATATAACCC TCGAACCGAT CAATGCCAGC
    CGGACGACCA GTTGCCTGAT CGAGGAGGTA GCCGAGCCGG ATTCACAGCC GGAGATTAAG
    CCGGTGGCAG AAGCGCAGTC TGCCAAAGTA TCCGAGGCTT GTGTCGAAAG TATTCTCCCC
    GAGACAGTTG AAAAGTTAGA AACAGCAGAC CAGGTCCAGC AGGTTATACC ACAGTTGGGG
    AAGCTTTACG TGGGCAGTAG CCAGCAGCAG TATGCGCAGC AGTCATCGCC CATCATCCAG
    GAGCCAGCTA CTCCTACTAT TTACGGAAAC AGCGCCGCAG CCGGAGCGCC GCAGTTCCCG
    CAGCCCGCCC AAAGGCAAGA GAAGCAGCCT CCGCAGCAGC AGCCCATCTA CGCTAACCAG
    TATGAGCTGA ATGTGGCCAA GGCGGCAGCT GCAGCATCTG TTTACTCACC CAGCTCCTCC
    ACCACCAGCA ACTCCAATCA ACAGCAGCAG CAGCAGCGCC GGAATGTGGC CCGTGGACTG
    CAGTATCGTG AATCTGGAGG ACTCGAGACT GGACGTGCTG GCAAGCAGCC ACCCAATGCG
    GGCATGTTGT CAATGGACAA CTTCCGTTTG CTAAGCGTTC TTGGTCGCGG CCACTTTGGC
    AAGGTGATTC TGTCCCAATT GCGAAGCAAC AACCAGTACT ACGCTATTAA GGCACTGAAG
    AAGGGAGACA TCATTGCCCG CGACGAAGTG GAGTCCCTGC TTAGCGAAAA GCGCATCTTC
    GAGGTGGCCA ACGCCATGCG CCATCCGTTC TTAGTTAACT TGTATTCGTG CTTCCAGACT
    GAGCAACACG TATGCTTTGT GATGGAATAC GCTGCTGGCG GAGATTTGAT GATGCACATC
    CACACGGACG TGTTCCTAGA GCCGAGAGCC GTTTTCTACG CCGCTTGTGT GGTTCTGGGC
    CTGCAGTACC TGCACGAGAA CAAGATCATC TACCGGGACC TGAAGCTGGA CAATTTGCTT
    TTGGACACGG AAGGATATGT GAAAATTGCG GACTTTGGTT TGTGCAAGGA GGGCATGGGC
    TTTGGTGATC GCACGGGCAC TTTCTGTGGT ACGCCCGAGT TTCTGGCACC GGAAGTGCTC
    ACGGAAACTT CCTACACACG AGCTGTGGAT TGGTGGGGCT TGGGTGTGTT GATCTTTGAG
    ATGTTGGTTG GTGAGTCCCC ATTCCCTGGT GACGATGAGG AGGAAGTATT CGATTCAATT
    GTCAACGATG AGGTGCGCTA TCCGCGCTTC CTATCGCTGG AGGCCATAGC CGTGATGCGT
    AGGCTTTTGC GCAAGAATCC AGAGAGACGT CTGGGATCTT CGGAACGGGA TGCGGAGGAT
    GTTAAGAAAC AGGCATTCTT CCGGTCAATT GTGTGGGATG ACCTGCTCCT GCGAAAGGTT
    AAACCACCAT TTGTGCCGAC AATTAACCAC TTGGAGGATG TGTCAAACTT TGACGAGGAG
    TTCACGTCGG AGAAGGCTCA GCTTACGCCA CCGAAGGAGC CGCGACACTT GACCGAGGAG
    GAGCAGTTGC TCTTCCAGGA CTTTTCATAC ACGGCCGAAT GGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246221.1
    CDS507..4334
    Translation

    Target ORF information:

    RefSeq Version NM_001259292.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant L (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259292.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGGCCCGTAT TAGTCTTGTA CATATAACCC TCGAACCGAT CAATGCCAGC
    CGGACGACCA GTTGCCTGAT CGAGGAGGTA GCCGAGCCGG ATTCACAGCC GGAGATTAAG
    CCGGTGGCAG AAGCGCAGTC TGCCAAAGTA TCCGAGGCTT GTGTCGAAAG TATTCTCCCC
    GAGACAGTTG AAAAGTTAGA AACAGCAGAC CAGGTCCAGC AGGTTATACC ACAGTTGGGG
    AAGCTTTACG TGGGCAGTAG CCAGCAGCAG TATGCGCAGC AGTCATCGCC CATCATCCAG
    GAGCCAGCTA CTCCTACTAT TTACGGAAAC AGCGCCGCAG CCGGAGCGCC GCAGTTCCCG
    CAGCCCGCCC AAAGGCAAGA GAAGCAGCCT CCGCAGCAGC AGCCCATCTA CGCTAACCAG
    TATGAGCTGA ATGTGGCCAA GGCGGCAGCT GCAGCATCTG TTTACTCACC CAGCTCCTCC
    ACCACCAGCA ACTCCAATCA ACAGCAGCAG CAGCAGCGCC GGAATGTGGC CCGTGGACTG
    CAGTATCGTG AATCTGGAGG ACTCGAGACT GGACGTGCTG GCAAGCAGCC ACCCAATGCG
    GGCATGTTGT CAATGGACAA CTTCCGTTTG CTAAGCGTTC TTGGTCGCGG CCACTTTGGC
    AAGGTGATTC TGTCCCAATT GCGAAGCAAC AACCAGTACT ACGCTATTAA GGCACTGAAG
    AAGGGAGACA TCATTGCCCG CGACGAAGTG GAGTCCCTGC TTAGCGAAAA GCGCATCTTC
    GAGGTGGCCA ACGCCATGCG CCATCCGTTC TTAGTTAACT TGTATTCGTG CTTCCAGACT
    GAGCAACACG TATGCTTTGT GATGGAATAC GCTGCTGGCG GAGATTTGAT GATGCACATC
    CACACGGACG TGTTCCTAGA GCCGAGAGCC GTTTTCTACG CCGCTTGTGT GGTTCTGGGC
    CTGCAGTACC TGCACGAGAA CAAGATCATC TACCGGGACC TGAAGCTGGA CAATTTGCTT
    TTGGACACGG AAGGATATGT GAAAATTGCG GACTTTGGTT TGTGCAAGGA GGGCATGGGC
    TTTGGTGATC GCACGGGCAC TTTCTGTGGT ACGCCCGAGT TTCTGGCACC GGAAGTGCTC
    ACGGAAACTT CCTACACACG AGCTGTGGAT TGGTGGGGCT TGGGTGTGTT GATCTTTGAG
    ATGTTGGTTG GTGAGTCCCC ATTCCCTGGT GACGATGAGG AGGAAGTATT CGATTCAATT
    GTCAACGATG AGGTGCGCTA TCCGCGCTTC CTATCGCTGG AGGCCATAGC CGTGATGCGT
    AGGCTTTTGC GCAAGAATCC AGAGAGACGT CTGGGATCTT CGGAACGGGA TGCGGAGGAT
    GTTAAGAAAC AGGCATTCTT CCGGTCAATT GTGTGGGATG ACCTGCTCCT GCGAAAGGTT
    AAACCACCAT TTGTGCCGAC AATTAACCAC TTGGAGGATG TGTCAAACTT TGACGAGGAG
    TTCACGTCGG AGAAGGCTCA GCTTACGCCA CCGAAGGAGC CGCGACACTT GACCGAGGAG
    GAGCAGTTGC TCTTCCAGGA CTTTTCATAC ACGGCCGAAT GGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12244
    Clone ID Related Accession (Same CDS sequence) NM_001169624.1
    Accession Version NM_001169624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGG TTATACCACA GTTGGGGAAG CTTTACGTGG GCAGTAGCCA GCAGCAGTAT
    GCGCAGCAGT CATCGCCCAT CATCCAGGAG CCAGCTACTC CTACTATTTA CGGAAACAGC
    GCCGCAGCCG GAGCGCCGCA GTTCCCGCAG CCCGCCCAAA GGCAAGAGAA GCAGCCTCCG
    CAGCAGCAGC CCATCTACGC TAACCAGTAT GAGCTGAATG TGGCCAAGGC GGCAGCTGCA
    GCATCTGTTT ACTCACCCAG CTCCTCCACC ACCAGCAACT CCAATCAACA GCAGCAGCAG
    CAGCGCCGGA ATGTGGCCCG TGGACTGCAG TATCGTGAAT CTGGAGGACT CGAGACTGGA
    CGTGCTGGCA AGCAGCCACC CAATGCGGGC ATGTTGTCAA TGGACAACTT CCGTTTGCTA
    AGCGTTCTTG GTCGCGGCCA CTTTGGCAAG GTGATTCTGT CCCAATTGCG AAGCAACAAC
    CAGTACTACG CTATTAAGGC ACTGAAGAAG GGAGACATCA TTGCCCGCGA CGAAGTGGAG
    TCCCTGCTTA GCGAAAAGCG CATCTTCGAG GTGGCCAACG CCATGCGCCA TCCGTTCTTA
    GTTAACTTGT ATTCGTGCTT CCAGACTGAG CAACACGTAT GCTTTGTGAT GGAATACGCT
    GCTGGCGGAG ATTTGATGAT GCACATCCAC ACGGACGTGT TCCTAGAGCC GAGAGCCGTT
    TTCTACGCCG CTTGTGTGGT TCTGGGCCTG CAGTACCTGC ACGAGAACAA GATCATCTAC
    CGGGACCTGA AGCTGGACAA TTTGCTTTTG GACACGGAAG GATATGTGAA AATTGCGGAC
    TTTGGTTTGT GCAAGGAGGG CATGGGCTTT GGTGATCGCA CGGGCACTTT CTGTGGTACG
    CCCGAGTTTC TGGCACCGGA AGTGCTCACG GAAACTTCCT ACACACGAGC TGTGGATTGG
    TGGGGCTTGG GTGTGTTGAT CTTTGAGATG TTGGTTGGTG AGTCCCCATT CCCTGGTGAC
    GATGAGGAGG AAGTATTCGA TTCAATTGTC AACGATGAGG TGCGCTATCC GCGCTTCCTA
    TCGCTGGAGG CCATAGCCGT GATGCGTAGG CTTTTGCGCA AGAATCCAGA GAGACGTCTG
    GGATCTTCGG AACGGGATGC GGAGGATGTT AAGAAACAGG CATTCTTCCG GTCAATTGTG
    TGGGATGACC TGCTCCTGCG AAAGGTTAAA CCACCATTTG TGCCGACAAT TAACCACTTG
    GAGGATGTGT CAAACTTTGA CGAGGAGTTC ACGTCGGAGA AGGCTCAGCT TACGCCACCG
    AAGGAGCCGC GACACTTGAC CGAGGAGGAG CAGTTGCTCT TCCAGGACTT TTCATACACG
    GCCGAATGGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163095.1
    CDS293..4147
    Translation

    Target ORF information:

    RefSeq Version NM_001169624.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant H (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGG TTATACCACA GTTGGGGAAG CTTTACGTGG GCAGTAGCCA GCAGCAGTAT
    GCGCAGCAGT CATCGCCCAT CATCCAGGAG CCAGCTACTC CTACTATTTA CGGAAACAGC
    GCCGCAGCCG GAGCGCCGCA GTTCCCGCAG CCCGCCCAAA GGCAAGAGAA GCAGCCTCCG
    CAGCAGCAGC CCATCTACGC TAACCAGTAT GAGCTGAATG TGGCCAAGGC GGCAGCTGCA
    GCATCTGTTT ACTCACCCAG CTCCTCCACC ACCAGCAACT CCAATCAACA GCAGCAGCAG
    CAGCGCCGGA ATGTGGCCCG TGGACTGCAG TATCGTGAAT CTGGAGGACT CGAGACTGGA
    CGTGCTGGCA AGCAGCCACC CAATGCGGGC ATGTTGTCAA TGGACAACTT CCGTTTGCTA
    AGCGTTCTTG GTCGCGGCCA CTTTGGCAAG GTGATTCTGT CCCAATTGCG AAGCAACAAC
    CAGTACTACG CTATTAAGGC ACTGAAGAAG GGAGACATCA TTGCCCGCGA CGAAGTGGAG
    TCCCTGCTTA GCGAAAAGCG CATCTTCGAG GTGGCCAACG CCATGCGCCA TCCGTTCTTA
    GTTAACTTGT ATTCGTGCTT CCAGACTGAG CAACACGTAT GCTTTGTGAT GGAATACGCT
    GCTGGCGGAG ATTTGATGAT GCACATCCAC ACGGACGTGT TCCTAGAGCC GAGAGCCGTT
    TTCTACGCCG CTTGTGTGGT TCTGGGCCTG CAGTACCTGC ACGAGAACAA GATCATCTAC
    CGGGACCTGA AGCTGGACAA TTTGCTTTTG GACACGGAAG GATATGTGAA AATTGCGGAC
    TTTGGTTTGT GCAAGGAGGG CATGGGCTTT GGTGATCGCA CGGGCACTTT CTGTGGTACG
    CCCGAGTTTC TGGCACCGGA AGTGCTCACG GAAACTTCCT ACACACGAGC TGTGGATTGG
    TGGGGCTTGG GTGTGTTGAT CTTTGAGATG TTGGTTGGTG AGTCCCCATT CCCTGGTGAC
    GATGAGGAGG AAGTATTCGA TTCAATTGTC AACGATGAGG TGCGCTATCC GCGCTTCCTA
    TCGCTGGAGG CCATAGCCGT GATGCGTAGG CTTTTGCGCA AGAATCCAGA GAGACGTCTG
    GGATCTTCGG AACGGGATGC GGAGGATGTT AAGAAACAGG CATTCTTCCG GTCAATTGTG
    TGGGATGACC TGCTCCTGCG AAAGGTTAAA CCACCATTTG TGCCGACAAT TAACCACTTG
    GAGGATGTGT CAAACTTTGA CGAGGAGTTC ACGTCGGAGA AGGCTCAGCT TACGCCACCG
    AAGGAGCCGC GACACTTGAC CGAGGAGGAG CAGTTGCTCT TCCAGGACTT TTCATACACG
    GCCGAATGGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12246
    Clone ID Related Accession (Same CDS sequence) NM_001259291.2
    Accession Version NM_001259291.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259291.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGGCC CGTATTAGTC TTGTACATAT AACCCTCGAA
    CCGATCAATG CCAGCCGGAC GACCAGTTGC CTGATCGAGG AGGTAGCCGA GCCGGATTCA
    CAGCCGGAGA TTAAGCCGGT GGCAGAAGCG CAGTCTGCCA AAGTATCCGA GGCTTGTGTC
    GAAAGTATTC TCCCCGAGAC AGTTGAAAAG TTAGAAACAG CAGACCAGGT CCAGCAGGTT
    ATACCACAGT TGGGGAAGCT TTACGTGGGC AGTAGCCAGC AGCAGTATGC GCAGCAGTCA
    TCGCCCATCA TCCAGGAGCC AGCTACTCCT ACTATTTACG GAAACAGCGC CGCAGCCGGA
    GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC
    ATCTACGCTA ACCAGTATGA GCTGAATGTG GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC
    TCACCCAGCT CCTCCACCAC CAGCAACTCC AATCAACAGC AGCAGCAGCA GCGCCGGAAT
    GTGGCCCGTG GACTGCAGTA TCGTGAATCT GGAGGACTCG AGACTGGACG TGCTGGCAAG
    CAGCCACCCA ATGCGGGCAT GTTGTCAATG GACAACTTCC GTTTGCTAAG CGTTCTTGGT
    CGCGGCCACT TTGGCAAGGT GATTCTGTCC CAATTGCGAA GCAACAACCA GTACTACGCT
    ATTAAGGCAC TGAAGAAGGG AGACATCATT GCCCGCGACG AAGTGGAGTC CCTGCTTAGC
    GAAAAGCGCA TCTTCGAGGT GGCCAACGCC ATGCGCCATC CGTTCTTAGT TAACTTGTAT
    TCGTGCTTCC AGACTGAGCA ACACGTATGC TTTGTGATGG AATACGCTGC TGGCGGAGAT
    TTGATGATGC ACATCCACAC GGACGTGTTC CTAGAGCCGA GAGCCGTTTT CTACGCCGCT
    TGTGTGGTTC TGGGCCTGCA GTACCTGCAC GAGAACAAGA TCATCTACCG GGACCTGAAG
    CTGGACAATT TGCTTTTGGA CACGGAAGGA TATGTGAAAA TTGCGGACTT TGGTTTGTGC
    AAGGAGGGCA TGGGCTTTGG TGATCGCACG GGCACTTTCT GTGGTACGCC CGAGTTTCTG
    GCACCGGAAG TGCTCACGGA AACTTCCTAC ACACGAGCTG TGGATTGGTG GGGCTTGGGT
    GTGTTGATCT TTGAGATGTT GGTTGGTGAG TCCCCATTCC CTGGTGACGA TGAGGAGGAA
    GTATTCGATT CAATTGTCAA CGATGAGGTG CGCTATCCGC GCTTCCTATC GCTGGAGGCC
    ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG AATCCAGAGA GACGTCTGGG ATCTTCGGAA
    CGGGATGCGG AGGATGTTAA GAAACAGGCA TTCTTCCGGT CAATTGTGTG GGATGACCTG
    CTCCTGCGAA AGGTTAAACC ACCATTTGTG CCGACAATTA ACCACTTGGA GGATGTGTCA
    AACTTTGACG AGGAGTTCAC GTCGGAGAAG GCTCAGCTTA CGCCACCGAA GGAGCCGCGA
    CACTTGACCG AGGAGGAGCA GTTGCTCTTC CAGGACTTTT CATACACGGC CGAATGGTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246220.1
    CDS421..4203
    Translation

    Target ORF information:

    RefSeq Version NM_001259291.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant M (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259291.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGGCC CGTATTAGTC TTGTACATAT AACCCTCGAA
    CCGATCAATG CCAGCCGGAC GACCAGTTGC CTGATCGAGG AGGTAGCCGA GCCGGATTCA
    CAGCCGGAGA TTAAGCCGGT GGCAGAAGCG CAGTCTGCCA AAGTATCCGA GGCTTGTGTC
    GAAAGTATTC TCCCCGAGAC AGTTGAAAAG TTAGAAACAG CAGACCAGGT CCAGCAGGTT
    ATACCACAGT TGGGGAAGCT TTACGTGGGC AGTAGCCAGC AGCAGTATGC GCAGCAGTCA
    TCGCCCATCA TCCAGGAGCC AGCTACTCCT ACTATTTACG GAAACAGCGC CGCAGCCGGA
    GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC
    ATCTACGCTA ACCAGTATGA GCTGAATGTG GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC
    TCACCCAGCT CCTCCACCAC CAGCAACTCC AATCAACAGC AGCAGCAGCA GCGCCGGAAT
    GTGGCCCGTG GACTGCAGTA TCGTGAATCT GGAGGACTCG AGACTGGACG TGCTGGCAAG
    CAGCCACCCA ATGCGGGCAT GTTGTCAATG GACAACTTCC GTTTGCTAAG CGTTCTTGGT
    CGCGGCCACT TTGGCAAGGT GATTCTGTCC CAATTGCGAA GCAACAACCA GTACTACGCT
    ATTAAGGCAC TGAAGAAGGG AGACATCATT GCCCGCGACG AAGTGGAGTC CCTGCTTAGC
    GAAAAGCGCA TCTTCGAGGT GGCCAACGCC ATGCGCCATC CGTTCTTAGT TAACTTGTAT
    TCGTGCTTCC AGACTGAGCA ACACGTATGC TTTGTGATGG AATACGCTGC TGGCGGAGAT
    TTGATGATGC ACATCCACAC GGACGTGTTC CTAGAGCCGA GAGCCGTTTT CTACGCCGCT
    TGTGTGGTTC TGGGCCTGCA GTACCTGCAC GAGAACAAGA TCATCTACCG GGACCTGAAG
    CTGGACAATT TGCTTTTGGA CACGGAAGGA TATGTGAAAA TTGCGGACTT TGGTTTGTGC
    AAGGAGGGCA TGGGCTTTGG TGATCGCACG GGCACTTTCT GTGGTACGCC CGAGTTTCTG
    GCACCGGAAG TGCTCACGGA AACTTCCTAC ACACGAGCTG TGGATTGGTG GGGCTTGGGT
    GTGTTGATCT TTGAGATGTT GGTTGGTGAG TCCCCATTCC CTGGTGACGA TGAGGAGGAA
    GTATTCGATT CAATTGTCAA CGATGAGGTG CGCTATCCGC GCTTCCTATC GCTGGAGGCC
    ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG AATCCAGAGA GACGTCTGGG ATCTTCGGAA
    CGGGATGCGG AGGATGTTAA GAAACAGGCA TTCTTCCGGT CAATTGTGTG GGATGACCTG
    CTCCTGCGAA AGGTTAAACC ACCATTTGTG CCGACAATTA ACCACTTGGA GGATGTGTCA
    AACTTTGACG AGGAGTTCAC GTCGGAGAAG GCTCAGCTTA CGCCACCGAA GGAGCCGCGA
    CACTTGACCG AGGAGGAGCA GTTGCTCTTC CAGGACTTTT CATACACGGC CGAATGGTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21740
    Clone ID Related Accession (Same CDS sequence) NM_001299315.1
    Accession Version NM_001299315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform Q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGCCG CAGCCACCAC ATCCCGCCCA GAAACCGACG
    AATCCCGAAC CACCGTCCGC TGTGGAACAG CAGCTGCACC GTCCGGTCCT AATCCTGCCG
    CCCGTGGTGC TCCTGGGTGG TCGGGAGGAG GACCGTTCGG TGCCACCATC GCCACTAGTC
    GAGTATCCCG AGGACGACGA CGAGTATCTG TATCGTGGTA GCAACGAAAA CCTGGGCACC
    AGGCTGCACT CCAGCCACAG TTCCAGTGCC GGCGATCGTT TCTGTGTGGA GGTTATACCA
    CAGTTGGGGA AGCTTTACGT GGGCAGTAGC CAGCAGCAGT ATGCGCAGCA GTCATCGCCC
    ATCATCCAGG AGCCAGCTAC TCCTACTATT TACGGAAACA GCGCCGCAGC CGGAGCGCCG
    CAGTTCCCGC AGCCCGCCCA AAGGCAAGAG AAGCAGCCTC CGCAGCAGCA GCCCATCTAC
    GCTAACCAGT ATGAGCTGAA TGTGGCCAAG GCGGCAGCTG CAGCATCTGT TTACTCACCC
    AGCTCCTCCA CCACCAGCAA CTCCAATCAA CAGCAGCAGC AGCAGCGCCG GAATGTGGCC
    CGTGGACTGC AGTATCGTGA ATCTGGAGGA CTCGAGACTG GACGTGCTGG CAAGCAGCCA
    CCCAATGCGG GCATGTTGTC AATGGACAAC TTCCGTTTGC TAAGCGTTCT TGGTCGCGGC
    CACTTTGGCA AGGTGATTCT GTCCCAATTG CGAAGCAACA ACCAGTACTA CGCTATTAAG
    GCACTGAAGA AGGGAGACAT CATTGCCCGC GACGAAGTGG AGTCCCTGCT TAGCGAAAAG
    CGCATCTTCG AGGTGGCCAA CGCCATGCGC CATCCGTTCT TAGTTAACTT GTATTCGTGC
    TTCCAGACTG AGCAACACGT ATGCTTTGTG ATGGAATACG CTGCTGGCGG AGATTTGATG
    ATGCACATCC ACACGGACGT GTTCCTAGAG CCGAGAGCCG TTTTCTACGC CGCTTGTGTG
    GTTCTGGGCC TGCAGTACCT GCACGAGAAC AAGATCATCT ACCGGGACCT GAAGCTGGAC
    AATTTGCTTT TGGACACGGA AGGATATGTG AAAATTGCGG ACTTTGGTTT GTGCAAGGAG
    GGCATGGGCT TTGGTGATCG CACGGGCACT TTCTGTGGTA CGCCCGAGTT TCTGGCACCG
    GAAGTGCTCA CGGAAACTTC CTACACACGA GCTGTGGATT GGTGGGGCTT GGGTGTGTTG
    ATCTTTGAGA TGTTGGTTGG TGAGTCCCCA TTCCCTGGTG ACGATGAGGA GGAAGTATTC
    GATTCAATTG TCAACGATGA GGTGCGCTAT CCGCGCTTCC TATCGCTGGA GGCCATAGCC
    GTGATGCGTA GGCTTTTGCG CAAGAATCCA GAGAGACGTC TGGGATCTTC GGAACGGGAT
    GCGGAGGATG TTAAGAAACA GGCATTCTTC CGGTCAATTG TGTGGGATGA CCTGCTCCTG
    CGAAAGGTTA AACCACCATT TGTGCCGACA ATTAACCACT TGGAGGATGT GTCAAACTTT
    GACGAGGAGT TCACGTCGGA GAAGGCTCAG CTTACGCCAC CGAAGGAGCC GCGACACTTG
    ACCGAGGAGG AGCAGTTGCT CTTCCAGGAC TTTTCATACA CGGCCGAATG GTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286244.1
    CDS421..4257
    Translation

    Target ORF information:

    RefSeq Version NM_001299315.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant Q (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGCCG CAGCCACCAC ATCCCGCCCA GAAACCGACG
    AATCCCGAAC CACCGTCCGC TGTGGAACAG CAGCTGCACC GTCCGGTCCT AATCCTGCCG
    CCCGTGGTGC TCCTGGGTGG TCGGGAGGAG GACCGTTCGG TGCCACCATC GCCACTAGTC
    GAGTATCCCG AGGACGACGA CGAGTATCTG TATCGTGGTA GCAACGAAAA CCTGGGCACC
    AGGCTGCACT CCAGCCACAG TTCCAGTGCC GGCGATCGTT TCTGTGTGGA GGTTATACCA
    CAGTTGGGGA AGCTTTACGT GGGCAGTAGC CAGCAGCAGT ATGCGCAGCA GTCATCGCCC
    ATCATCCAGG AGCCAGCTAC TCCTACTATT TACGGAAACA GCGCCGCAGC CGGAGCGCCG
    CAGTTCCCGC AGCCCGCCCA AAGGCAAGAG AAGCAGCCTC CGCAGCAGCA GCCCATCTAC
    GCTAACCAGT ATGAGCTGAA TGTGGCCAAG GCGGCAGCTG CAGCATCTGT TTACTCACCC
    AGCTCCTCCA CCACCAGCAA CTCCAATCAA CAGCAGCAGC AGCAGCGCCG GAATGTGGCC
    CGTGGACTGC AGTATCGTGA ATCTGGAGGA CTCGAGACTG GACGTGCTGG CAAGCAGCCA
    CCCAATGCGG GCATGTTGTC AATGGACAAC TTCCGTTTGC TAAGCGTTCT TGGTCGCGGC
    CACTTTGGCA AGGTGATTCT GTCCCAATTG CGAAGCAACA ACCAGTACTA CGCTATTAAG
    GCACTGAAGA AGGGAGACAT CATTGCCCGC GACGAAGTGG AGTCCCTGCT TAGCGAAAAG
    CGCATCTTCG AGGTGGCCAA CGCCATGCGC CATCCGTTCT TAGTTAACTT GTATTCGTGC
    TTCCAGACTG AGCAACACGT ATGCTTTGTG ATGGAATACG CTGCTGGCGG AGATTTGATG
    ATGCACATCC ACACGGACGT GTTCCTAGAG CCGAGAGCCG TTTTCTACGC CGCTTGTGTG
    GTTCTGGGCC TGCAGTACCT GCACGAGAAC AAGATCATCT ACCGGGACCT GAAGCTGGAC
    AATTTGCTTT TGGACACGGA AGGATATGTG AAAATTGCGG ACTTTGGTTT GTGCAAGGAG
    GGCATGGGCT TTGGTGATCG CACGGGCACT TTCTGTGGTA CGCCCGAGTT TCTGGCACCG
    GAAGTGCTCA CGGAAACTTC CTACACACGA GCTGTGGATT GGTGGGGCTT GGGTGTGTTG
    ATCTTTGAGA TGTTGGTTGG TGAGTCCCCA TTCCCTGGTG ACGATGAGGA GGAAGTATTC
    GATTCAATTG TCAACGATGA GGTGCGCTAT CCGCGCTTCC TATCGCTGGA GGCCATAGCC
    GTGATGCGTA GGCTTTTGCG CAAGAATCCA GAGAGACGTC TGGGATCTTC GGAACGGGAT
    GCGGAGGATG TTAAGAAACA GGCATTCTTC CGGTCAATTG TGTGGGATGA CCTGCTCCTG
    CGAAAGGTTA AACCACCATT TGTGCCGACA ATTAACCACT TGGAGGATGT GTCAAACTTT
    GACGAGGAGT TCACGTCGGA GAAGGCTCAG CTTACGCCAC CGAAGGAGCC GCGACACTTG
    ACCGAGGAGG AGCAGTTGCT CTTCCAGGAC TTTTCATACA CGGCCGAATG GTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21741
    Clone ID Related Accession (Same CDS sequence) NM_001299314.1
    Accession Version NM_001299314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGTTG CAGCCGCAGC CACCACATCC CGCCCAGAAA
    CCGACGAATC CCGAACCACC GTCCGCTGTG GAACAGCAGC TGCACCGTCC GGTCCTAATC
    CTGCCGCCCG TGGTGCTCCT GGGTGGTCGG GAGGAGGACC GTTCGGTGCC ACCATCGCCA
    CTAGTCGAGT ATCCCGAGGA CGACGACGAG TATCTGTATC GTGGTAGCAA CGAAAACCTG
    GGCACCAGGC TGCACTCCAG CCACAGTTCC AGTGCCGGCG ATCGTTTCTG TGTGGAGGTT
    ATACCACAGT TGGGGAAGCT TTACGTGGGC AGTAGCCAGC AGCAGTATGC GCAGCAGTCA
    TCGCCCATCA TCCAGGAGCC AGCTACTCCT ACTATTTACG GAAACAGCGC CGCAGCCGGA
    GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC
    ATCTACGCTA ACCAGTATGA GCTGAATGTG GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC
    TCACCCAGCT CCTCCACCAC CAGCAACTCC AATCAACAGC AGCAGCAGCA GCGCCGGAAT
    GTGGCCCGTG GACTGCAGTA TCGTGAATCT GGAGGACTCG AGACTGGACG TGCTGGCAAG
    CAGCCACCCA ATGCGGGCAT GTTGTCAATG GACAACTTCC GTTTGCTAAG CGTTCTTGGT
    CGCGGCCACT TTGGCAAGGT GATTCTGTCC CAATTGCGAA GCAACAACCA GTACTACGCT
    ATTAAGGCAC TGAAGAAGGG AGACATCATT GCCCGCGACG AAGTGGAGTC CCTGCTTAGC
    GAAAAGCGCA TCTTCGAGGT GGCCAACGCC ATGCGCCATC CGTTCTTAGT TAACTTGTAT
    TCGTGCTTCC AGACTGAGCA ACACGTATGC TTTGTGATGG AATACGCTGC TGGCGGAGAT
    TTGATGATGC ACATCCACAC GGACGTGTTC CTAGAGCCGA GAGCCGTTTT CTACGCCGCT
    TGTGTGGTTC TGGGCCTGCA GTACCTGCAC GAGAACAAGA TCATCTACCG GGACCTGAAG
    CTGGACAATT TGCTTTTGGA CACGGAAGGA TATGTGAAAA TTGCGGACTT TGGTTTGTGC
    AAGGAGGGCA TGGGCTTTGG TGATCGCACG GGCACTTTCT GTGGTACGCC CGAGTTTCTG
    GCACCGGAAG TGCTCACGGA AACTTCCTAC ACACGAGCTG TGGATTGGTG GGGCTTGGGT
    GTGTTGATCT TTGAGATGTT GGTTGGTGAG TCCCCATTCC CTGGTGACGA TGAGGAGGAA
    GTATTCGATT CAATTGTCAA CGATGAGGTG CGCTATCCGC GCTTCCTATC GCTGGAGGCC
    ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG AATCCAGAGA GACGTCTGGG ATCTTCGGAA
    CGGGATGCGG AGGATGTTAA GAAACAGGCA TTCTTCCGGT CAATTGTGTG GGATGACCTG
    CTCCTGCGAA AGGTTAAACC ACCATTTGTG CCGACAATTA ACCACTTGGA GGATGTGTCA
    AACTTTGACG AGGAGTTCAC GTCGGAGAAG GCTCAGCTTA CGCCACCGAA GGAGCCGCGA
    CACTTGACCG AGGAGGAGCA GTTGCTCTTC CAGGACTTTT CATACACGGC CGAATGGTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286243.1
    CDS421..4263
    Translation

    Target ORF information:

    RefSeq Version NM_001299314.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant P (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGTTG CAGCCGCAGC CACCACATCC CGCCCAGAAA
    CCGACGAATC CCGAACCACC GTCCGCTGTG GAACAGCAGC TGCACCGTCC GGTCCTAATC
    CTGCCGCCCG TGGTGCTCCT GGGTGGTCGG GAGGAGGACC GTTCGGTGCC ACCATCGCCA
    CTAGTCGAGT ATCCCGAGGA CGACGACGAG TATCTGTATC GTGGTAGCAA CGAAAACCTG
    GGCACCAGGC TGCACTCCAG CCACAGTTCC AGTGCCGGCG ATCGTTTCTG TGTGGAGGTT
    ATACCACAGT TGGGGAAGCT TTACGTGGGC AGTAGCCAGC AGCAGTATGC GCAGCAGTCA
    TCGCCCATCA TCCAGGAGCC AGCTACTCCT ACTATTTACG GAAACAGCGC CGCAGCCGGA
    GCGCCGCAGT TCCCGCAGCC CGCCCAAAGG CAAGAGAAGC AGCCTCCGCA GCAGCAGCCC
    ATCTACGCTA ACCAGTATGA GCTGAATGTG GCCAAGGCGG CAGCTGCAGC ATCTGTTTAC
    TCACCCAGCT CCTCCACCAC CAGCAACTCC AATCAACAGC AGCAGCAGCA GCGCCGGAAT
    GTGGCCCGTG GACTGCAGTA TCGTGAATCT GGAGGACTCG AGACTGGACG TGCTGGCAAG
    CAGCCACCCA ATGCGGGCAT GTTGTCAATG GACAACTTCC GTTTGCTAAG CGTTCTTGGT
    CGCGGCCACT TTGGCAAGGT GATTCTGTCC CAATTGCGAA GCAACAACCA GTACTACGCT
    ATTAAGGCAC TGAAGAAGGG AGACATCATT GCCCGCGACG AAGTGGAGTC CCTGCTTAGC
    GAAAAGCGCA TCTTCGAGGT GGCCAACGCC ATGCGCCATC CGTTCTTAGT TAACTTGTAT
    TCGTGCTTCC AGACTGAGCA ACACGTATGC TTTGTGATGG AATACGCTGC TGGCGGAGAT
    TTGATGATGC ACATCCACAC GGACGTGTTC CTAGAGCCGA GAGCCGTTTT CTACGCCGCT
    TGTGTGGTTC TGGGCCTGCA GTACCTGCAC GAGAACAAGA TCATCTACCG GGACCTGAAG
    CTGGACAATT TGCTTTTGGA CACGGAAGGA TATGTGAAAA TTGCGGACTT TGGTTTGTGC
    AAGGAGGGCA TGGGCTTTGG TGATCGCACG GGCACTTTCT GTGGTACGCC CGAGTTTCTG
    GCACCGGAAG TGCTCACGGA AACTTCCTAC ACACGAGCTG TGGATTGGTG GGGCTTGGGT
    GTGTTGATCT TTGAGATGTT GGTTGGTGAG TCCCCATTCC CTGGTGACGA TGAGGAGGAA
    GTATTCGATT CAATTGTCAA CGATGAGGTG CGCTATCCGC GCTTCCTATC GCTGGAGGCC
    ATAGCCGTGA TGCGTAGGCT TTTGCGCAAG AATCCAGAGA GACGTCTGGG ATCTTCGGAA
    CGGGATGCGG AGGATGTTAA GAAACAGGCA TTCTTCCGGT CAATTGTGTG GGATGACCTG
    CTCCTGCGAA AGGTTAAACC ACCATTTGTG CCGACAATTA ACCACTTGGA GGATGTGTCA
    AACTTTGACG AGGAGTTCAC GTCGGAGAAG GCTCAGCTTA CGCCACCGAA GGAGCCGCGA
    CACTTGACCG AGGAGGAGCA GTTGCTCTTC CAGGACTTTT CATACACGGC CGAATGGTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09371
    Clone ID Related Accession (Same CDS sequence) NM_176113.3
    Accession Version NM_176113.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_176113.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGCTGCAGGA TGCCCTGCAC GAATTCGATT TCCTGTCGGA CCTGGACTCA
    CGTCCCACCA CGCTGCGTCG CCTGCTGAAG GAGCAGAAGA TGGCGCTGGA TGCCGGCGCG
    CTGGAGAATC TGCTGCTGCA GCAGCAACAG ACTGAGCAGC AGGCACTGCA GCAGCGCCAG
    CGCCAGGAGC AGCTGCGTCT GCAGCAGTTT CAGGAGGCGC AGCGCCAGGC GATCCTGGAC
    CTGTGCGTGA AACAGAGAGA GCCCGCGGAG CAGACATCAC CCACCCTTGC CAACCAAACG
    CCGACGTTCC CACCGCTTGC CAGCAATCAG ATCATGCCAA GCTTAAATGC CCGCCCTTGC
    CAAAGCCAAA GCCCAAACCA CAACGAATTT CAGTTGCAGC CGCAGCCACC ACATCCCGCC
    CAGAAACCGA CGAATCCCGA ACCACCGTCC GCTGTGGAAC AGCAGCTGCA CCGTCCGGTC
    CTAATCCTGC CGCCCGTGGT GCTCCTGGGT GGTCGGGAGG AGGACCGTTC GGTGCCACCA
    TCGCCACTAG TCGAGTATCC CGAGGACGAC GACGAGTATC TGTATCGTGG TAGCAACGAA
    AACCTGGGCA CCAGGCTGCA CTCCAGCCAC AGTTCCAGTG CCGGCGATCG TTTCTGTGTG
    GAGGTTATAC CACAGTTGGG GAAGCTTTAC GTGGGCAGTA GCCAGCAGCA GTATGCGCAG
    CAGTCATCGC CCATCATCCA GGAGCCAGCT ACTCCTACTA TTTACGGAAA CAGCGCCGCA
    GCCGGAGCGC CGCAGTTCCC GCAGCCCGCC CAAAGGCAAG AGAAGCAGCC TCCGCAGCAG
    CAGCCCATCT ACGCTAACCA GTATGAGCTG AATGTGGCCA AGGCGGCAGC TGCAGCATCT
    GTTTACTCAC CCAGCTCCTC CACCACCAGC AACTCCAATC AACAGCAGCA GCAGCAGCGC
    CGGAATGTGG CCCGTGGACT GCAGTATCGT GAATCTGGAG GACTCGAGAC TGGACGTGCT
    GGCAAGCAGC CACCCAATGC GGGCATGTTG TCAATGGACA ACTTCCGTTT GCTAAGCGTT
    CTTGGTCGCG GCCACTTTGG CAAGGTGATT CTGTCCCAAT TGCGAAGCAA CAACCAGTAC
    TACGCTATTA AGGCACTGAA GAAGGGAGAC ATCATTGCCC GCGACGAAGT GGAGTCCCTG
    CTTAGCGAAA AGCGCATCTT CGAGGTGGCC AACGCCATGC GCCATCCGTT CTTAGTTAAC
    TTGTATTCGT GCTTCCAGAC TGAGCAACAC GTATGCTTTG TGATGGAATA CGCTGCTGGC
    GGAGATTTGA TGATGCACAT CCACACGGAC GTGTTCCTAG AGCCGAGAGC CGTTTTCTAC
    GCCGCTTGTG TGGTTCTGGG CCTGCAGTAC CTGCACGAGA ACAAGATCAT CTACCGGGAC
    CTGAAGCTGG ACAATTTGCT TTTGGACACG GAAGGATATG TGAAAATTGC GGACTTTGGT
    TTGTGCAAGG AGGGCATGGG CTTTGGTGAT CGCACGGGCA CTTTCTGTGG TACGCCCGAG
    TTTCTGGCAC CGGAAGTGCT CACGGAAACT TCCTACACAC GAGCTGTGGA TTGGTGGGGC
    TTGGGTGTGT TGATCTTTGA GATGTTGGTT GGTGAGTCCC CATTCCCTGG TGACGATGAG
    GAGGAAGTAT TCGATTCAAT TGTCAACGAT GAGGTGCGCT ATCCGCGCTT CCTATCGCTG
    GAGGCCATAG CCGTGATGCG TAGGCTTTTG CGCAAGAATC CAGAGAGACG TCTGGGATCT
    TCGGAACGGG ATGCGGAGGA TGTTAAGAAA CAGGCATTCT TCCGGTCAAT TGTGTGGGAT
    GACCTGCTCC TGCGAAAGGT TAAACCACCA TTTGTGCCGA CAATTAACCA CTTGGAGGAT
    GTGTCAAACT TTGACGAGGA GTTCACGTCG GAGAAGGCTC AGCTTACGCC ACCGAAGGAG
    CCGCGACACT TGACCGAGGA GGAGCAGTTG CTCTTCCAGG ACTTTTCATA CACGGCCGAA
    TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788293.2
    CDS498..4766
    Translation

    Target ORF information:

    RefSeq Version NM_176113.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant D (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176113.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCATTGA CCATGAACAT GGTTTTCCTC AAGGACCTAC GATCGCGCCT CAAGGGATAT 
    CTGCACGGCG AATACATCAA GCATCCCGTT CTGTACGAGC TCAGTCACAA ATATGGTTTC
    ACAGAGAATC TGCCGGAGAG CTGTATGTCC ATACGGCTGG AGGAGATCAA GGAGGCCATT
    CGGCGAGAGA TCCGCAAGGA GCTGAAGATC AAAGAGGGCG CCGAGAAGCT CCGCGAGGTG
    GCTAAAGATC GACGATCCCT CAGCGATGTG GCCGTTCTTG TCAAGAAGAG CAAAAGTAAA
    CTTGCCGAGC TGAAGTCCGA GTTGCAGGAG CTCGAGAGTC AAATCCTCCT GACATCGGCC
    AACACCGCCG TCAATAGTAA TGGACAAGAA TCGATCACTG CCTGCATTGA TCCCAATGGC
    GGCTTCTTGG TCAGCGGTGC AGTTGGTGGC TTGGGCGGCG GAAACACGGC TCTGGAGGGC
    GGCGCACCGG CCACTGCCAA TGACAAAGTG CTCGCCTCGC TGGAGAAGCA GCTGCAGATC
    GAGATGAAGG TGAAGACCGG GGCGGAAAAC ATGATCCAGT CGCTGGGCAT CGGATGCGAC
    AAGAAGCTGC TGGCGGAAGC CCACCAGATG CTGGCCGACT CGAAGGCCAA GATTGAGTTT
    TTGCGCCTGC GCATCATCAA GGTGAAACAG AACCGCGAGC AGGCCGATCG CCTCAAGGCC
    TCGCGCCAGA TGATCGACGA GCATGGCCAG ACGATCGGCG GGAACAACAG CAGCCAGCCG
    CAGAGCCTGG AGACGACGCT GGAGGAGCGA ATCGAGGAGC TCCGCCATCG ACTGCGAATC
    GAGGCAGCCG TCGTCGATGG AGCCAAGAAT GTTATACGCA CGCTGCAGAC GGCGAATAGG
    GCACCGGACA AGAAGGCTTT GCAGGAGGCC CATGGACGTT TGTCGGAATC GTCGCGAAAA
    CTAGATCTCT TGCGCTACTC CTTGGATTTA CGACGCCAGG AGCTGCCCGC CGATTCGCCC
    GCCGCCCAGC AATTAAAAAC GGAGCTGCAG ATCGTCCAGC TATCGACCTC CCCAGCTCCG
    GTCACCTACA CGTCACTGCA GTCCGGACAA GCGGGCATAC TCGGCGGAAA GCCGTACCAG
    TCGGTATCCT CGCTTGGACG CTGTGCCAGT GTCACCGGAA AACTGGAGGT TCGCTTGCTG
    GGCTGTCAAG ATTTGCTGGA AGATGTGCCC GGTAGATCGA GGAGGGACAA GGACAACAAC
    TCAAGTCCGG GTGATTTACG AAGCTTCGTC AAGGGCGTCA CGTCGCGCAG CAGTTCAAAG
    AGCTATTCGG TAAAGGATGA GACGTCCATT GAGATCATGG CGGTCATCAA GCTGGACAAT
    ATCACCGTTG GCCAGACATC GTGGAAGCAA TGCTCGCAGC AGGCCTGGGA TCAGCGCTTC
    TCCATCGATC TAGACCGGTC GCGCGAACTG GAGATTGGAG TTTACTGGCG CGACTGGCGA
    TCCCTGTGCG CCGTAAAGGT GCTGCGCCTT GAAGAGTTCA TCGACGATGT ACGACATGGC
    ATGGCATTGC AGCTGGAGCC ACAAGGTCTG CTATTTGCGG AGGTCAAGTT CTTGAACCCC
    ATGATTTCAC AGAAGCCAAA GCTGAGGCGC CAGCGTATGA TCTTCAACAG GCAGCAAGCG
    AAGAACATCT CGCGGGCCAA GCAGATGAAC ATTAATGTGG CCACATGGGG CCGCCTGCTT
    AAGCGGAATG CTCCTAACCA TGTGCACATG GGATCGGCAG GATCAGGATC TTCTCTAACC
    GGTAGCTCGC CTATGGTGGT TGGTGGGTCT CGCGACTCTG AGTCTCCGAT TTCGAGGACT
    CCCTCATCCG ACGCACTGGT GGAACCGGAG CCCTACACGC CCGGAGAGCA GGCACAGAAC
    TTGGAATTCG ATCCCGATGC AGGAATTAAT GAACACGTGG AGACGCCAGG TGAATACCCG
    GATCCGGCGG CCAGTGGTCT GAGCGGAATG CGACCCCTGT CCATGCACAT GCAGGGAATT
    AGTGTGCTGC CCCCGGAATC GCCACCCGTG GCCACCGGAG CAGCTGGTCG GCCCAATACG
    CTCAGCTTAC AGATGCCGGG TGCCAGTAAA GGACAGGTGA TCCAAGGCGG ACGGACTGCG
    GCGCCTACAA CGGCGCCACC GCCACCACCT GTGCTCAAGG CGACATCCAC CACTCCGATA
    TTGGATCAGG AGCTGCAGGA TGCCCTGCAC GAATTCGATT TCCTGTCGGA CCTGGACTCA
    CGTCCCACCA CGCTGCGTCG CCTGCTGAAG GAGCAGAAGA TGGCGCTGGA TGCCGGCGCG
    CTGGAGAATC TGCTGCTGCA GCAGCAACAG ACTGAGCAGC AGGCACTGCA GCAGCGCCAG
    CGCCAGGAGC AGCTGCGTCT GCAGCAGTTT CAGGAGGCGC AGCGCCAGGC GATCCTGGAC
    CTGTGCGTGA AACAGAGAGA GCCCGCGGAG CAGACATCAC CCACCCTTGC CAACCAAACG
    CCGACGTTCC CACCGCTTGC CAGCAATCAG ATCATGCCAA GCTTAAATGC CCGCCCTTGC
    CAAAGCCAAA GCCCAAACCA CAACGAATTT CAGTTGCAGC CGCAGCCACC ACATCCCGCC
    CAGAAACCGA CGAATCCCGA ACCACCGTCC GCTGTGGAAC AGCAGCTGCA CCGTCCGGTC
    CTAATCCTGC CGCCCGTGGT GCTCCTGGGT GGTCGGGAGG AGGACCGTTC GGTGCCACCA
    TCGCCACTAG TCGAGTATCC CGAGGACGAC GACGAGTATC TGTATCGTGG TAGCAACGAA
    AACCTGGGCA CCAGGCTGCA CTCCAGCCAC AGTTCCAGTG CCGGCGATCG TTTCTGTGTG
    GAGGTTATAC CACAGTTGGG GAAGCTTTAC GTGGGCAGTA GCCAGCAGCA GTATGCGCAG
    CAGTCATCGC CCATCATCCA GGAGCCAGCT ACTCCTACTA TTTACGGAAA CAGCGCCGCA
    GCCGGAGCGC CGCAGTTCCC GCAGCCCGCC CAAAGGCAAG AGAAGCAGCC TCCGCAGCAG
    CAGCCCATCT ACGCTAACCA GTATGAGCTG AATGTGGCCA AGGCGGCAGC TGCAGCATCT
    GTTTACTCAC CCAGCTCCTC CACCACCAGC AACTCCAATC AACAGCAGCA GCAGCAGCGC
    CGGAATGTGG CCCGTGGACT GCAGTATCGT GAATCTGGAG GACTCGAGAC TGGACGTGCT
    GGCAAGCAGC CACCCAATGC GGGCATGTTG TCAATGGACA ACTTCCGTTT GCTAAGCGTT
    CTTGGTCGCG GCCACTTTGG CAAGGTGATT CTGTCCCAAT TGCGAAGCAA CAACCAGTAC
    TACGCTATTA AGGCACTGAA GAAGGGAGAC ATCATTGCCC GCGACGAAGT GGAGTCCCTG
    CTTAGCGAAA AGCGCATCTT CGAGGTGGCC AACGCCATGC GCCATCCGTT CTTAGTTAAC
    TTGTATTCGT GCTTCCAGAC TGAGCAACAC GTATGCTTTG TGATGGAATA CGCTGCTGGC
    GGAGATTTGA TGATGCACAT CCACACGGAC GTGTTCCTAG AGCCGAGAGC CGTTTTCTAC
    GCCGCTTGTG TGGTTCTGGG CCTGCAGTAC CTGCACGAGA ACAAGATCAT CTACCGGGAC
    CTGAAGCTGG ACAATTTGCT TTTGGACACG GAAGGATATG TGAAAATTGC GGACTTTGGT
    TTGTGCAAGG AGGGCATGGG CTTTGGTGAT CGCACGGGCA CTTTCTGTGG TACGCCCGAG
    TTTCTGGCAC CGGAAGTGCT CACGGAAACT TCCTACACAC GAGCTGTGGA TTGGTGGGGC
    TTGGGTGTGT TGATCTTTGA GATGTTGGTT GGTGAGTCCC CATTCCCTGG TGACGATGAG
    GAGGAAGTAT TCGATTCAAT TGTCAACGAT GAGGTGCGCT ATCCGCGCTT CCTATCGCTG
    GAGGCCATAG CCGTGATGCG TAGGCTTTTG CGCAAGAATC CAGAGAGACG TCTGGGATCT
    TCGGAACGGG ATGCGGAGGA TGTTAAGAAA CAGGCATTCT TCCGGTCAAT TGTGTGGGAT
    GACCTGCTCC TGCGAAAGGT TAAACCACCA TTTGTGCCGA CAATTAACCA CTTGGAGGAT
    GTGTCAAACT TTGACGAGGA GTTCACGTCG GAGAAGGCTC AGCTTACGCC ACCGAAGGAG
    CCGCGACACT TGACCGAGGA GGAGCAGTTG CTCTTCCAGG ACTTTTCATA CACGGCCGAA
    TGGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09372
    Clone ID Related Accession (Same CDS sequence) NM_001103767.2
    Accession Version NM_001103767.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_001103767.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGC TGCAGGATGC CCTGCACGAA TTCGATTTCC TGTCGGACCT GGACTCACGT
    CCCACCACGC TGCGTCGCCT GCTGAAGGAG CAGAAGATGG CGCTGGATGC CGGCGCGCTG
    GAGAATCTGC TGCTGCAGCA GCAACAGACT GAGCAGCAGG CACTGCAGCA GCGCCAGCGC
    CAGGAGCAGC TGCGTCTGCA GCAGTTTCAG GAGGCGCAGC GCCAGGCGAT CCTGGACCTG
    TGCGTGAAAC AGAGAGAGCC CGCGGAGCAG ACATCACCCA CCCTTGCCAA CCAAACGCCG
    ACGTTCCCAC CGCTTGCCAG CAATCAGATC ATGCCAAGCT TAAATGCCCG CCCTTGCCAA
    AGCCAAAGCC CAAACCACAA CGAATTTCAG TTGCAGCCGC AGCCACCACA TCCCGCCCAG
    AAACCGACGA ATCCCGAACC ACCGTCCGCT GTGGAACAGC AGCTGCACCG TCCGGTCCTA
    ATCCTGCCGC CCGTGGTGCT CCTGGGTGGT CGGGAGGAGG ACCGTTCGGT GCCACCATCG
    CCACTAGTCG AGTATCCCGA GGACGACGAC GAGTATCTGT ATCGTGGTAG CAACGAAAAC
    CTGGGCACCA GGCTGCACTC CAGCCACAGT TCCAGTGCCG GCGATCGTTT CTGTGTGGAG
    GTTATACCAC AGTTGGGGAA GCTTTACGTG GGCAGTAGCC AGCAGCAGTA TGCGCAGCAG
    TCATCGCCCA TCATCCAGGA GCCAGCTACT CCTACTATTT ACGGAAACAG CGCCGCAGCC
    GGAGCGCCGC AGTTCCCGCA GCCCGCCCAA AGGCAAGAGA AGCAGCCTCC GCAGCAGCAG
    CCCATCTACG CTAACCAGTA TGAGCTGAAT GTGGCCAAGG CGGCAGCTGC AGCATCTGTT
    TACTCACCCA GCTCCTCCAC CACCAGCAAC TCCAATCAAC AGCAGCAGCA GCAGCGCCGG
    AATGTGGCCC GTGGACTGCA GTATCGTGAA TCTGGAGGAC TCGAGACTGG ACGTGCTGGC
    AAGCAGCCAC CCAATGCGGG CATGTTGTCA ATGGACAACT TCCGTTTGCT AAGCGTTCTT
    GGTCGCGGCC ACTTTGGCAA GGTGATTCTG TCCCAATTGC GAAGCAACAA CCAGTACTAC
    GCTATTAAGG CACTGAAGAA GGGAGACATC ATTGCCCGCG ACGAAGTGGA GTCCCTGCTT
    AGCGAAAAGC GCATCTTCGA GGTGGCCAAC GCCATGCGCC ATCCGTTCTT AGTTAACTTG
    TATTCGTGCT TCCAGACTGA GCAACACGTA TGCTTTGTGA TGGAATACGC TGCTGGCGGA
    GATTTGATGA TGCACATCCA CACGGACGTG TTCCTAGAGC CGAGAGCCGT TTTCTACGCC
    GCTTGTGTGG TTCTGGGCCT GCAGTACCTG CACGAGAACA AGATCATCTA CCGGGACCTG
    AAGCTGGACA ATTTGCTTTT GGACACGGAA GGATATGTGA AAATTGCGGA CTTTGGTTTG
    TGCAAGGAGG GCATGGGCTT TGGTGATCGC ACGGGCACTT TCTGTGGTAC GCCCGAGTTT
    CTGGCACCGG AAGTGCTCAC GGAAACTTCC TACACACGAG CTGTGGATTG GTGGGGCTTG
    GGTGTGTTGA TCTTTGAGAT GTTGGTTGGT GAGTCCCCAT TCCCTGGTGA CGATGAGGAG
    GAAGTATTCG ATTCAATTGT CAACGATGAG GTGCGCTATC CGCGCTTCCT ATCGCTGGAG
    GCCATAGCCG TGATGCGTAG GCTTTTGCGC AAGAATCCAG AGAGACGTCT GGGATCTTCG
    GAACGGGATG CGGAGGATGT TAAGAAACAG GCATTCTTCC GGTCAATTGT GTGGGATGAC
    CTGCTCCTGC GAAAGGTTAA ACCACCATTT GTGCCGACAA TTAACCACTT GGAGGATGTG
    TCAAACTTTG ACGAGGAGTT CACGTCGGAG AAGGCTCAGC TTACGCCACC GAAGGAGCCG
    CGACACTTGA CCGAGGAGGA GCAGTTGCTC TTCCAGGACT TTTCATACAC GGCCGAATGG
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097237.1
    CDS293..4798
    Translation

    Target ORF information:

    RefSeq Version NM_001103767.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant G (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103767.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGC TGCAGGATGC CCTGCACGAA TTCGATTTCC TGTCGGACCT GGACTCACGT
    CCCACCACGC TGCGTCGCCT GCTGAAGGAG CAGAAGATGG CGCTGGATGC CGGCGCGCTG
    GAGAATCTGC TGCTGCAGCA GCAACAGACT GAGCAGCAGG CACTGCAGCA GCGCCAGCGC
    CAGGAGCAGC TGCGTCTGCA GCAGTTTCAG GAGGCGCAGC GCCAGGCGAT CCTGGACCTG
    TGCGTGAAAC AGAGAGAGCC CGCGGAGCAG ACATCACCCA CCCTTGCCAA CCAAACGCCG
    ACGTTCCCAC CGCTTGCCAG CAATCAGATC ATGCCAAGCT TAAATGCCCG CCCTTGCCAA
    AGCCAAAGCC CAAACCACAA CGAATTTCAG TTGCAGCCGC AGCCACCACA TCCCGCCCAG
    AAACCGACGA ATCCCGAACC ACCGTCCGCT GTGGAACAGC AGCTGCACCG TCCGGTCCTA
    ATCCTGCCGC CCGTGGTGCT CCTGGGTGGT CGGGAGGAGG ACCGTTCGGT GCCACCATCG
    CCACTAGTCG AGTATCCCGA GGACGACGAC GAGTATCTGT ATCGTGGTAG CAACGAAAAC
    CTGGGCACCA GGCTGCACTC CAGCCACAGT TCCAGTGCCG GCGATCGTTT CTGTGTGGAG
    GTTATACCAC AGTTGGGGAA GCTTTACGTG GGCAGTAGCC AGCAGCAGTA TGCGCAGCAG
    TCATCGCCCA TCATCCAGGA GCCAGCTACT CCTACTATTT ACGGAAACAG CGCCGCAGCC
    GGAGCGCCGC AGTTCCCGCA GCCCGCCCAA AGGCAAGAGA AGCAGCCTCC GCAGCAGCAG
    CCCATCTACG CTAACCAGTA TGAGCTGAAT GTGGCCAAGG CGGCAGCTGC AGCATCTGTT
    TACTCACCCA GCTCCTCCAC CACCAGCAAC TCCAATCAAC AGCAGCAGCA GCAGCGCCGG
    AATGTGGCCC GTGGACTGCA GTATCGTGAA TCTGGAGGAC TCGAGACTGG ACGTGCTGGC
    AAGCAGCCAC CCAATGCGGG CATGTTGTCA ATGGACAACT TCCGTTTGCT AAGCGTTCTT
    GGTCGCGGCC ACTTTGGCAA GGTGATTCTG TCCCAATTGC GAAGCAACAA CCAGTACTAC
    GCTATTAAGG CACTGAAGAA GGGAGACATC ATTGCCCGCG ACGAAGTGGA GTCCCTGCTT
    AGCGAAAAGC GCATCTTCGA GGTGGCCAAC GCCATGCGCC ATCCGTTCTT AGTTAACTTG
    TATTCGTGCT TCCAGACTGA GCAACACGTA TGCTTTGTGA TGGAATACGC TGCTGGCGGA
    GATTTGATGA TGCACATCCA CACGGACGTG TTCCTAGAGC CGAGAGCCGT TTTCTACGCC
    GCTTGTGTGG TTCTGGGCCT GCAGTACCTG CACGAGAACA AGATCATCTA CCGGGACCTG
    AAGCTGGACA ATTTGCTTTT GGACACGGAA GGATATGTGA AAATTGCGGA CTTTGGTTTG
    TGCAAGGAGG GCATGGGCTT TGGTGATCGC ACGGGCACTT TCTGTGGTAC GCCCGAGTTT
    CTGGCACCGG AAGTGCTCAC GGAAACTTCC TACACACGAG CTGTGGATTG GTGGGGCTTG
    GGTGTGTTGA TCTTTGAGAT GTTGGTTGGT GAGTCCCCAT TCCCTGGTGA CGATGAGGAG
    GAAGTATTCG ATTCAATTGT CAACGATGAG GTGCGCTATC CGCGCTTCCT ATCGCTGGAG
    GCCATAGCCG TGATGCGTAG GCTTTTGCGC AAGAATCCAG AGAGACGTCT GGGATCTTCG
    GAACGGGATG CGGAGGATGT TAAGAAACAG GCATTCTTCC GGTCAATTGT GTGGGATGAC
    CTGCTCCTGC GAAAGGTTAA ACCACCATTT GTGCCGACAA TTAACCACTT GGAGGATGTG
    TCAAACTTTG ACGAGGAGTT CACGTCGGAG AAGGCTCAGC TTACGCCACC GAAGGAGCCG
    CGACACTTGA CCGAGGAGGA GCAGTTGCTC TTCCAGGACT TTTCATACAC GGCCGAATGG
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa09374
    Clone ID Related Accession (Same CDS sequence) NM_176111.2
    Accession Version NM_176111.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4224bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_176111.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGCTG CAGGATGCCC TGCACGAATT CGATTTCCTG
    TCGGACCTGG ACTCACGTCC CACCACGCTG CGTCGCCTGC TGAAGGAGCA GAAGATGGCG
    CTGGATGCCG GCGCGCTGGA GAATCTGCTG CTGCAGCAGC AACAGACTGA GCAGCAGGCA
    CTGCAGCAGC GCCAGCGCCA GGAGCAGCTG CGTCTGCAGC AGTTTCAGGA GGCGCAGCGC
    CAGGCGATCC TGGACCTGTG CGTGAAACAG AGAGAGCCCG CGGAGCAGAC ATCACCCACC
    CTTGCCAACC AAACGCCGAC GTTCCCACCG CTTGCCAGCA ATCAGATCAT GCCAAGCTTA
    AATGCCCGCC CTTGCCAAAG CCAAAGCCCA AACCACAACG AATTTCAGTT GCAGCCGCAG
    CCACCACATC CCGCCCAGAA ACCGACGAAT CCCGAACCAC CGTCCGCTGT GGAACAGCAG
    CTGCACCGTC CGGTCCTAAT CCTGCCGCCC GTGGTGCTCC TGGGTGGTCG GGAGGAGGAC
    CGTTCGGTGC CACCATCGCC ACTAGTCGAG TATCCCGAGG ACGACGACGA GTATCTGTAT
    CGTGGTAGCA ACGAAAACCT GGGCACCAGG CTGCACTCCA GCCACAGTTC CAGTGCCGGC
    GATCGTTTCT GTGTGGAGGT TATACCACAG TTGGGGAAGC TTTACGTGGG CAGTAGCCAG
    CAGCAGTATG CGCAGCAGTC ATCGCCCATC ATCCAGGAGC CAGCTACTCC TACTATTTAC
    GGAAACAGCG CCGCAGCCGG AGCGCCGCAG TTCCCGCAGC CCGCCCAAAG GCAAGAGAAG
    CAGCCTCCGC AGCAGCAGCC CATCTACGCT AACCAGTATG AGCTGAATGT GGCCAAGGCG
    GCAGCTGCAG CATCTGTTTA CTCACCCAGC TCCTCCACCA CCAGCAACTC CAATCAACAG
    CAGCAGCAGC AGCGCCGGAA TGTGGCCCGT GGACTGCAGT ATCGTGAATC TGGAGGACTC
    GAGACTGGAC GTGCTGGCAA GCAGCCACCC AATGCGGGCA TGTTGTCAAT GGACAACTTC
    CGTTTGCTAA GCGTTCTTGG TCGCGGCCAC TTTGGCAAGG TGATTCTGTC CCAATTGCGA
    AGCAACAACC AGTACTACGC TATTAAGGCA CTGAAGAAGG GAGACATCAT TGCCCGCGAC
    GAAGTGGAGT CCCTGCTTAG CGAAAAGCGC ATCTTCGAGG TGGCCAACGC CATGCGCCAT
    CCGTTCTTAG TTAACTTGTA TTCGTGCTTC CAGACTGAGC AACACGTATG CTTTGTGATG
    GAATACGCTG CTGGCGGAGA TTTGATGATG CACATCCACA CGGACGTGTT CCTAGAGCCG
    AGAGCCGTTT TCTACGCCGC TTGTGTGGTT CTGGGCCTGC AGTACCTGCA CGAGAACAAG
    ATCATCTACC GGGACCTGAA GCTGGACAAT TTGCTTTTGG ACACGGAAGG ATATGTGAAA
    ATTGCGGACT TTGGTTTGTG CAAGGAGGGC ATGGGCTTTG GTGATCGCAC GGGCACTTTC
    TGTGGTACGC CCGAGTTTCT GGCACCGGAA GTGCTCACGG AAACTTCCTA CACACGAGCT
    GTGGATTGGT GGGGCTTGGG TGTGTTGATC TTTGAGATGT TGGTTGGTGA GTCCCCATTC
    CCTGGTGACG ATGAGGAGGA AGTATTCGAT TCAATTGTCA ACGATGAGGT GCGCTATCCG
    CGCTTCCTAT CGCTGGAGGC CATAGCCGTG ATGCGTAGGC TTTTGCGCAA GAATCCAGAG
    AGACGTCTGG GATCTTCGGA ACGGGATGCG GAGGATGTTA AGAAACAGGC ATTCTTCCGG
    TCAATTGTGT GGGATGACCT GCTCCTGCGA AAGGTTAAAC CACCATTTGT GCCGACAATT
    AACCACTTGG AGGATGTGTC AAACTTTGAC GAGGAGTTCA CGTCGGAGAA GGCTCAGCTT
    ACGCCACCGA AGGAGCCGCG ACACTTGACC GAGGAGGAGC AGTTGCTCTT CCAGGACTTT
    TCATACACGG CCGAATGGTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788291.1
    CDS421..4644
    Translation

    Target ORF information:

    RefSeq Version NM_176111.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant C (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176111.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGCTG CAGGATGCCC TGCACGAATT CGATTTCCTG
    TCGGACCTGG ACTCACGTCC CACCACGCTG CGTCGCCTGC TGAAGGAGCA GAAGATGGCG
    CTGGATGCCG GCGCGCTGGA GAATCTGCTG CTGCAGCAGC AACAGACTGA GCAGCAGGCA
    CTGCAGCAGC GCCAGCGCCA GGAGCAGCTG CGTCTGCAGC AGTTTCAGGA GGCGCAGCGC
    CAGGCGATCC TGGACCTGTG CGTGAAACAG AGAGAGCCCG CGGAGCAGAC ATCACCCACC
    CTTGCCAACC AAACGCCGAC GTTCCCACCG CTTGCCAGCA ATCAGATCAT GCCAAGCTTA
    AATGCCCGCC CTTGCCAAAG CCAAAGCCCA AACCACAACG AATTTCAGTT GCAGCCGCAG
    CCACCACATC CCGCCCAGAA ACCGACGAAT CCCGAACCAC CGTCCGCTGT GGAACAGCAG
    CTGCACCGTC CGGTCCTAAT CCTGCCGCCC GTGGTGCTCC TGGGTGGTCG GGAGGAGGAC
    CGTTCGGTGC CACCATCGCC ACTAGTCGAG TATCCCGAGG ACGACGACGA GTATCTGTAT
    CGTGGTAGCA ACGAAAACCT GGGCACCAGG CTGCACTCCA GCCACAGTTC CAGTGCCGGC
    GATCGTTTCT GTGTGGAGGT TATACCACAG TTGGGGAAGC TTTACGTGGG CAGTAGCCAG
    CAGCAGTATG CGCAGCAGTC ATCGCCCATC ATCCAGGAGC CAGCTACTCC TACTATTTAC
    GGAAACAGCG CCGCAGCCGG AGCGCCGCAG TTCCCGCAGC CCGCCCAAAG GCAAGAGAAG
    CAGCCTCCGC AGCAGCAGCC CATCTACGCT AACCAGTATG AGCTGAATGT GGCCAAGGCG
    GCAGCTGCAG CATCTGTTTA CTCACCCAGC TCCTCCACCA CCAGCAACTC CAATCAACAG
    CAGCAGCAGC AGCGCCGGAA TGTGGCCCGT GGACTGCAGT ATCGTGAATC TGGAGGACTC
    GAGACTGGAC GTGCTGGCAA GCAGCCACCC AATGCGGGCA TGTTGTCAAT GGACAACTTC
    CGTTTGCTAA GCGTTCTTGG TCGCGGCCAC TTTGGCAAGG TGATTCTGTC CCAATTGCGA
    AGCAACAACC AGTACTACGC TATTAAGGCA CTGAAGAAGG GAGACATCAT TGCCCGCGAC
    GAAGTGGAGT CCCTGCTTAG CGAAAAGCGC ATCTTCGAGG TGGCCAACGC CATGCGCCAT
    CCGTTCTTAG TTAACTTGTA TTCGTGCTTC CAGACTGAGC AACACGTATG CTTTGTGATG
    GAATACGCTG CTGGCGGAGA TTTGATGATG CACATCCACA CGGACGTGTT CCTAGAGCCG
    AGAGCCGTTT TCTACGCCGC TTGTGTGGTT CTGGGCCTGC AGTACCTGCA CGAGAACAAG
    ATCATCTACC GGGACCTGAA GCTGGACAAT TTGCTTTTGG ACACGGAAGG ATATGTGAAA
    ATTGCGGACT TTGGTTTGTG CAAGGAGGGC ATGGGCTTTG GTGATCGCAC GGGCACTTTC
    TGTGGTACGC CCGAGTTTCT GGCACCGGAA GTGCTCACGG AAACTTCCTA CACACGAGCT
    GTGGATTGGT GGGGCTTGGG TGTGTTGATC TTTGAGATGT TGGTTGGTGA GTCCCCATTC
    CCTGGTGACG ATGAGGAGGA AGTATTCGAT TCAATTGTCA ACGATGAGGT GCGCTATCCG
    CGCTTCCTAT CGCTGGAGGC CATAGCCGTG ATGCGTAGGC TTTTGCGCAA GAATCCAGAG
    AGACGTCTGG GATCTTCGGA ACGGGATGCG GAGGATGTTA AGAAACAGGC ATTCTTCCGG
    TCAATTGTGT GGGATGACCT GCTCCTGCGA AAGGTTAAAC CACCATTTGT GCCGACAATT
    AACCACTTGG AGGATGTGTC AAACTTTGAC GAGGAGTTCA CGTCGGAGAA GGCTCAGCTT
    ACGCCACCGA AGGAGCCGCG ACACTTGACC GAGGAGGAGC AGTTGCTCTT CCAGGACTTT
    TCATACACGG CCGAATGGTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12245
    Clone ID Related Accession (Same CDS sequence) NM_001201968.3 , NM_001201967.1
    Accession Version NM_001201968.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001201968.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGG CCCGTATTAG TCTTGTACAT ATAACCCTCG AACCGATCAA TGCCAGCCGG
    ACGACCAGTT GCCTGATCGA GGAGGTAGCC GAGCCGGATT CACAGCCGGA GATTAAGCCG
    GTGGCAGAAG CGCAGTCTGC CAAAGTATCC GAGGCTTGTG TCGAAAGTAT TCTCCCCGAG
    ACAGTTGAAA AGTTAGAAAC AGCAGACCAG GTCCAGCAGG TTATACCACA GTTGGGGAAG
    CTTTACGTGG GCAGTAGCCA GCAGCAGTAT GCGCAGCAGT CATCGCCCAT CATCCAGGAG
    CCAGCTACTC CTACTATTTA CGGAAACAGC GCCGCAGCCG GAGCGCCGCA GTTCCCGCAG
    CCCGCCCAAA GGCAAGAGAA GCAGCCTCCG CAGCAGCAGC CCATCTACGC TAACCAGTAT
    GAGCTGAATG TGGCCAAGGC GGCAGCTGCA GCATCTGTTT ACTCACCCAG CTCCTCCACC
    ACCAGCAACT CCAATCAACA GCAGCAGCAG CAGCGCCGGA ATGTGGCCCG TGGACTGCAG
    TATCGTGAAT CTGGAGGACT CGAGACTGGA CGTGCTGGCA AGCAGCCACC CAATGCGGGC
    ATGTTGTCAA TGGACAACTT CCGTTTGCTA AGCGTTCTTG GTCGCGGCCA CTTTGGCAAG
    GTGATTCTGT CCCAATTGCG AAGCAACAAC CAGTACTACG CTATTAAGGC ACTGAAGAAG
    GGAGACATCA TTGCCCGCGA CGAAGTGGAG TCCCTGCTTA GCGAAAAGCG CATCTTCGAG
    GTGGCCAACG CCATGCGCCA TCCGTTCTTA GTTAACTTGT ATTCGTGCTT CCAGACTGAG
    CAACACGTAT GCTTTGTGAT GGAATACGCT GCTGGCGGAG ATTTGATGAT GCACATCCAC
    ACGGACGTGT TCCTAGAGCC GAGAGCCGTT TTCTACGCCG CTTGTGTGGT TCTGGGCCTG
    CAGTACCTGC ACGAGAACAA GATCATCTAC CGGGACCTGA AGCTGGACAA TTTGCTTTTG
    GACACGGAAG GATATGTGAA AATTGCGGAC TTTGGTTTGT GCAAGGAGGG CATGGGCTTT
    GGTGATCGCA CGGGCACTTT CTGTGGTACG CCCGAGTTTC TGGCACCGGA AGTGCTCACG
    GAAACTTCCT ACACACGAGC TGTGGATTGG TGGGGCTTGG GTGTGTTGAT CTTTGAGATG
    TTGGTTGGTG AGTCCCCATT CCCTGGTGAC GATGAGGAGG AAGTATTCGA TTCAATTGTC
    AACGATGAGG TGCGCTATCC GCGCTTCCTA TCGCTGGAGG CCATAGCCGT GATGCGTAGG
    CTTTTGCGCA AGAATCCAGA GAGACGTCTG GGATCTTCGG AACGGGATGC GGAGGATGTT
    AAGAAACAGG CATTCTTCCG GTCAATTGTG TGGGATGACC TGCTCCTGCG AAAGGTTAAA
    CCACCATTTG TGCCGACAAT TAACCACTTG GAGGATGTGT CAAACTTTGA CGAGGAGTTC
    ACGTCGGAGA AGGCTCAGCT TACGCCACCG AAGGAGCCGC GACACTTGAC CGAGGAGGAG
    CAGTTGCTCT TCCAGGACTT TTCATACACG GCCGAATGGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188897.3
    CDS198..4262
    Translation

    Target ORF information:

    RefSeq Version NM_001201968.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant S (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201968.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGG CCCGTATTAG TCTTGTACAT ATAACCCTCG AACCGATCAA TGCCAGCCGG
    ACGACCAGTT GCCTGATCGA GGAGGTAGCC GAGCCGGATT CACAGCCGGA GATTAAGCCG
    GTGGCAGAAG CGCAGTCTGC CAAAGTATCC GAGGCTTGTG TCGAAAGTAT TCTCCCCGAG
    ACAGTTGAAA AGTTAGAAAC AGCAGACCAG GTCCAGCAGG TTATACCACA GTTGGGGAAG
    CTTTACGTGG GCAGTAGCCA GCAGCAGTAT GCGCAGCAGT CATCGCCCAT CATCCAGGAG
    CCAGCTACTC CTACTATTTA CGGAAACAGC GCCGCAGCCG GAGCGCCGCA GTTCCCGCAG
    CCCGCCCAAA GGCAAGAGAA GCAGCCTCCG CAGCAGCAGC CCATCTACGC TAACCAGTAT
    GAGCTGAATG TGGCCAAGGC GGCAGCTGCA GCATCTGTTT ACTCACCCAG CTCCTCCACC
    ACCAGCAACT CCAATCAACA GCAGCAGCAG CAGCGCCGGA ATGTGGCCCG TGGACTGCAG
    TATCGTGAAT CTGGAGGACT CGAGACTGGA CGTGCTGGCA AGCAGCCACC CAATGCGGGC
    ATGTTGTCAA TGGACAACTT CCGTTTGCTA AGCGTTCTTG GTCGCGGCCA CTTTGGCAAG
    GTGATTCTGT CCCAATTGCG AAGCAACAAC CAGTACTACG CTATTAAGGC ACTGAAGAAG
    GGAGACATCA TTGCCCGCGA CGAAGTGGAG TCCCTGCTTA GCGAAAAGCG CATCTTCGAG
    GTGGCCAACG CCATGCGCCA TCCGTTCTTA GTTAACTTGT ATTCGTGCTT CCAGACTGAG
    CAACACGTAT GCTTTGTGAT GGAATACGCT GCTGGCGGAG ATTTGATGAT GCACATCCAC
    ACGGACGTGT TCCTAGAGCC GAGAGCCGTT TTCTACGCCG CTTGTGTGGT TCTGGGCCTG
    CAGTACCTGC ACGAGAACAA GATCATCTAC CGGGACCTGA AGCTGGACAA TTTGCTTTTG
    GACACGGAAG GATATGTGAA AATTGCGGAC TTTGGTTTGT GCAAGGAGGG CATGGGCTTT
    GGTGATCGCA CGGGCACTTT CTGTGGTACG CCCGAGTTTC TGGCACCGGA AGTGCTCACG
    GAAACTTCCT ACACACGAGC TGTGGATTGG TGGGGCTTGG GTGTGTTGAT CTTTGAGATG
    TTGGTTGGTG AGTCCCCATT CCCTGGTGAC GATGAGGAGG AAGTATTCGA TTCAATTGTC
    AACGATGAGG TGCGCTATCC GCGCTTCCTA TCGCTGGAGG CCATAGCCGT GATGCGTAGG
    CTTTTGCGCA AGAATCCAGA GAGACGTCTG GGATCTTCGG AACGGGATGC GGAGGATGTT
    AAGAAACAGG CATTCTTCCG GTCAATTGTG TGGGATGACC TGCTCCTGCG AAAGGTTAAA
    CCACCATTTG TGCCGACAAT TAACCACTTG GAGGATGTGT CAAACTTTGA CGAGGAGTTC
    ACGTCGGAGA AGGCTCAGCT TACGCCACCG AAGGAGCCGC GACACTTGAC CGAGGAGGAG
    CAGTTGCTCT TCCAGGACTT TTCATACACG GCCGAATGGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12245
    Clone ID Related Accession (Same CDS sequence) NM_001201968.3 , NM_001201967.1
    Accession Version NM_001201967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGG CCCGTATTAG TCTTGTACAT ATAACCCTCG AACCGATCAA TGCCAGCCGG
    ACGACCAGTT GCCTGATCGA GGAGGTAGCC GAGCCGGATT CACAGCCGGA GATTAAGCCG
    GTGGCAGAAG CGCAGTCTGC CAAAGTATCC GAGGCTTGTG TCGAAAGTAT TCTCCCCGAG
    ACAGTTGAAA AGTTAGAAAC AGCAGACCAG GTCCAGCAGG TTATACCACA GTTGGGGAAG
    CTTTACGTGG GCAGTAGCCA GCAGCAGTAT GCGCAGCAGT CATCGCCCAT CATCCAGGAG
    CCAGCTACTC CTACTATTTA CGGAAACAGC GCCGCAGCCG GAGCGCCGCA GTTCCCGCAG
    CCCGCCCAAA GGCAAGAGAA GCAGCCTCCG CAGCAGCAGC CCATCTACGC TAACCAGTAT
    GAGCTGAATG TGGCCAAGGC GGCAGCTGCA GCATCTGTTT ACTCACCCAG CTCCTCCACC
    ACCAGCAACT CCAATCAACA GCAGCAGCAG CAGCGCCGGA ATGTGGCCCG TGGACTGCAG
    TATCGTGAAT CTGGAGGACT CGAGACTGGA CGTGCTGGCA AGCAGCCACC CAATGCGGGC
    ATGTTGTCAA TGGACAACTT CCGTTTGCTA AGCGTTCTTG GTCGCGGCCA CTTTGGCAAG
    GTGATTCTGT CCCAATTGCG AAGCAACAAC CAGTACTACG CTATTAAGGC ACTGAAGAAG
    GGAGACATCA TTGCCCGCGA CGAAGTGGAG TCCCTGCTTA GCGAAAAGCG CATCTTCGAG
    GTGGCCAACG CCATGCGCCA TCCGTTCTTA GTTAACTTGT ATTCGTGCTT CCAGACTGAG
    CAACACGTAT GCTTTGTGAT GGAATACGCT GCTGGCGGAG ATTTGATGAT GCACATCCAC
    ACGGACGTGT TCCTAGAGCC GAGAGCCGTT TTCTACGCCG CTTGTGTGGT TCTGGGCCTG
    CAGTACCTGC ACGAGAACAA GATCATCTAC CGGGACCTGA AGCTGGACAA TTTGCTTTTG
    GACACGGAAG GATATGTGAA AATTGCGGAC TTTGGTTTGT GCAAGGAGGG CATGGGCTTT
    GGTGATCGCA CGGGCACTTT CTGTGGTACG CCCGAGTTTC TGGCACCGGA AGTGCTCACG
    GAAACTTCCT ACACACGAGC TGTGGATTGG TGGGGCTTGG GTGTGTTGAT CTTTGAGATG
    TTGGTTGGTG AGTCCCCATT CCCTGGTGAC GATGAGGAGG AAGTATTCGA TTCAATTGTC
    AACGATGAGG TGCGCTATCC GCGCTTCCTA TCGCTGGAGG CCATAGCCGT GATGCGTAGG
    CTTTTGCGCA AGAATCCAGA GAGACGTCTG GGATCTTCGG AACGGGATGC GGAGGATGTT
    AAGAAACAGG CATTCTTCCG GTCAATTGTG TGGGATGACC TGCTCCTGCG AAAGGTTAAA
    CCACCATTTG TGCCGACAAT TAACCACTTG GAGGATGTGT CAAACTTTGA CGAGGAGTTC
    ACGTCGGAGA AGGCTCAGCT TACGCCACCG AAGGAGCCGC GACACTTGAC CGAGGAGGAG
    CAGTTGCTCT TCCAGGACTT TTCATACACG GCCGAATGGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188896.1
    CDS293..4357
    Translation

    Target ORF information:

    RefSeq Version NM_001201967.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant K (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAAAC TGGTGAAGAG AATCCGCAGC ATGATCAATC CAAGCAGCCA GCGGGATCGC 
    GAAGATACCA CCTCGGTATC CGCTTCAGAG GGCTTCATTG CTCGCAGGAA GTGCCACTCG
    CTGCCCCGAA GGAGCAATAG ACACTCGACC GCCCGGCGAT CAAACAGTGG TCTGTGGAAT
    CGACTGGTCA CCAATGTCTT CGGACCGGAA GATTCATATG ACTTTGACAA CGAAGGCGAC
    AGTGGTGGTG CCGTATTTTA CACCGATTCG GTGAACGGTT CAAACTATGA GATATCTGCA
    GAAGGCGAAT ACATCAAGCA TCCCGTTCTG TACGAGCTCA GTCACAAATA TGGTTTCACA
    GAGAATCTGC CGGAGAGCTG TATGTCCATA CGGCTGGAGG AGATCAAGGA GGCCATTCGG
    CGAGAGATCC GCAAGGAGCT GAAGATCAAA GAGGGCGCCG AGAAGCTCCG CGAGGTGGCT
    AAAGATCGAC GATCCCTCAG CGATGTGGCC GTTCTTGTCA AGAAGAGCAA AAGTAAACTT
    GCCGAGCTGA AGTCCGAGTT GCAGGAGCTC GAGAGTCAAA TCCTCCTGAC ATCGGCCAAC
    ACCGCCGTCA ATAGTAATGG ACAAGAATCG ATCACTGCCT GCATTGATCC CAATGGCGGC
    TTCTTGGTCA GCGGTGCAGT TGGTGGCTTG GGCGGCGGAA ACACGGCTCT GGAGGGCGGC
    GCACCGGCCA CTGCCAATGA CAAAGTGCTC GCCTCGCTGG AGAAGCAGCT GCAGATCGAG
    ATGAAGGTGA AGACCGGGGC GGAAAACATG ATCCAGTCGC TGGGCATCGG ATGCGACAAG
    AAGCTGCTGG CGGAAGCCCA CCAGATGCTG GCCGACTCGA AGGCCAAGAT TGAGTTTTTG
    CGCCTGCGCA TCATCAAGGT GAAACAGAAC CGCGAGCAGG CCGATCGCCT CAAGGCCTCG
    CGCCAGATGA TCGACGAGCA TGGCCAGACG ATCGGCGGGA ACAACAGCAG CCAGCCGCAG
    AGCCTGGAGA CGACGCTGGA GGAGCGAATC GAGGAGCTCC GCCATCGACT GCGAATCGAG
    GCAGCCGTCG TCGATGGAGC CAAGAATGTT ATACGCACGC TGCAGACGGC GAATAGGGCA
    CCGGACAAGA AGGCTTTGCA GGAGGCCCAT GGACGTTTGT CGGAATCGTC GCGAAAACTA
    GATCTCTTGC GCTACTCCTT GGATTTACGA CGCCAGGAGC TGCCCGCCGA TTCGCCCGCC
    GCCCAGCAAT TAAAAACGGA GCTGCAGATC GTCCAGCTAT CGACCTCCCC AGCTCCGGTC
    ACCTACACGT CACTGCAGTC CGGACAAGCG GGCATACTCG GCGGAAAGCC GTACCAGTCG
    GTATCCTCGC TTGGACGCTG TGCCAGTGTC ACCGGAAAAC TGGAGGTTCG CTTGCTGGGC
    TGTCAAGATT TGCTGGAAGA TGTGCCCGGT AGATCGAGGA GGGACAAGGA CAACAACTCA
    AGTCCGGGTG ATTTACGAAG CTTCGTCAAG GGCGTCACGT CGCGCAGCAG TTCAAAGAGC
    TATTCGGTAA AGGATGAGAC GTCCATTGAG ATCATGGCGG TCATCAAGCT GGACAATATC
    ACCGTTGGCC AGACATCGTG GAAGCAATGC TCGCAGCAGG CCTGGGATCA GCGCTTCTCC
    ATCGATCTAG ACCGGTCGCG CGAACTGGAG ATTGGAGTTT ACTGGCGCGA CTGGCGATCC
    CTGTGCGCCG TAAAGGTGCT GCGCCTTGAA GAGTTCATCG ACGATGTACG ACATGGCATG
    GCATTGCAGC TGGAGCCACA AGGTCTGCTA TTTGCGGAGG TCAAGTTCTT GAACCCCATG
    ATTTCACAGA AGCCAAAGCT GAGGCGCCAG CGTATGATCT TCAACAGGCA GCAAGCGAAG
    AACATCTCGC GGGCCAAGCA GATGAACATT AATGTGGCCA CATGGGGCCG CCTGCTTAAG
    CGGAATGCTC CTAACCATGT GCACATGGGA TCGGCAGGAT CAGGATCTTC TCTAACCGGT
    AGCTCGCCTA TGGTGGTTGG TGGGTCTCGC GACTCTGAGT CTCCGATTTC GAGGACTCCC
    TCATCCGACG CACTGGTGGA ACCGGAGCCC TACACGCCCG GAGAGCAGGC ACAGAACTTG
    GAATTCGATC CCGATGCAGG AATTAATGAA CACGTGGAGA CGCCAGGTGA ATACCCGGAT
    CCGGCGGCCA GTGGTCTGAG CGGAATGCGA CCCCTGTCCA TGCACATGCA GGGAATTAGT
    GTGCTGCCCC CGGAATCGCC ACCCGTGGCC ACCGGAGCAG CTGGTCGGCC CAATACGCTC
    AGCTTACAGA TGCCGGGTGC CAGTAAAGGA CAGGTGATCC AAGGCGGACG GACTGCGGCG
    CCTACAACGG CGCCACCGCC ACCACCTGTG CTCAAGGCGA CATCCACCAC TCCGATATTG
    GATCAGGAGG CCCGTATTAG TCTTGTACAT ATAACCCTCG AACCGATCAA TGCCAGCCGG
    ACGACCAGTT GCCTGATCGA GGAGGTAGCC GAGCCGGATT CACAGCCGGA GATTAAGCCG
    GTGGCAGAAG CGCAGTCTGC CAAAGTATCC GAGGCTTGTG TCGAAAGTAT TCTCCCCGAG
    ACAGTTGAAA AGTTAGAAAC AGCAGACCAG GTCCAGCAGG TTATACCACA GTTGGGGAAG
    CTTTACGTGG GCAGTAGCCA GCAGCAGTAT GCGCAGCAGT CATCGCCCAT CATCCAGGAG
    CCAGCTACTC CTACTATTTA CGGAAACAGC GCCGCAGCCG GAGCGCCGCA GTTCCCGCAG
    CCCGCCCAAA GGCAAGAGAA GCAGCCTCCG CAGCAGCAGC CCATCTACGC TAACCAGTAT
    GAGCTGAATG TGGCCAAGGC GGCAGCTGCA GCATCTGTTT ACTCACCCAG CTCCTCCACC
    ACCAGCAACT CCAATCAACA GCAGCAGCAG CAGCGCCGGA ATGTGGCCCG TGGACTGCAG
    TATCGTGAAT CTGGAGGACT CGAGACTGGA CGTGCTGGCA AGCAGCCACC CAATGCGGGC
    ATGTTGTCAA TGGACAACTT CCGTTTGCTA AGCGTTCTTG GTCGCGGCCA CTTTGGCAAG
    GTGATTCTGT CCCAATTGCG AAGCAACAAC CAGTACTACG CTATTAAGGC ACTGAAGAAG
    GGAGACATCA TTGCCCGCGA CGAAGTGGAG TCCCTGCTTA GCGAAAAGCG CATCTTCGAG
    GTGGCCAACG CCATGCGCCA TCCGTTCTTA GTTAACTTGT ATTCGTGCTT CCAGACTGAG
    CAACACGTAT GCTTTGTGAT GGAATACGCT GCTGGCGGAG ATTTGATGAT GCACATCCAC
    ACGGACGTGT TCCTAGAGCC GAGAGCCGTT TTCTACGCCG CTTGTGTGGT TCTGGGCCTG
    CAGTACCTGC ACGAGAACAA GATCATCTAC CGGGACCTGA AGCTGGACAA TTTGCTTTTG
    GACACGGAAG GATATGTGAA AATTGCGGAC TTTGGTTTGT GCAAGGAGGG CATGGGCTTT
    GGTGATCGCA CGGGCACTTT CTGTGGTACG CCCGAGTTTC TGGCACCGGA AGTGCTCACG
    GAAACTTCCT ACACACGAGC TGTGGATTGG TGGGGCTTGG GTGTGTTGAT CTTTGAGATG
    TTGGTTGGTG AGTCCCCATT CCCTGGTGAC GATGAGGAGG AAGTATTCGA TTCAATTGTC
    AACGATGAGG TGCGCTATCC GCGCTTCCTA TCGCTGGAGG CCATAGCCGT GATGCGTAGG
    CTTTTGCGCA AGAATCCAGA GAGACGTCTG GGATCTTCGG AACGGGATGC GGAGGATGTT
    AAGAAACAGG CATTCTTCCG GTCAATTGTG TGGGATGACC TGCTCCTGCG AAAGGTTAAA
    CCACCATTTG TGCCGACAAT TAACCACTTG GAGGATGTGT CAAACTTTGA CGAGGAGTTC
    ACGTCGGAGA AGGCTCAGCT TACGCCACCG AAGGAGCCGC GACACTTGAC CGAGGAGGAG
    CAGTTGCTCT TCCAGGACTT TTCATACACG GCCGAATGGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21742
    Clone ID Related Accession (Same CDS sequence) NM_001299312.1
    Accession Version NM_001299312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product protein kinase N, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGCTG CAGGATGCCC TGCACGAATT CGATTTCCTG
    TCGGACCTGG ACTCACGTCC CACCACGCTG CGTCGCCTGC TGAAGGAGCA GAAGATGGCG
    CTGGATGCCG GCGCGCTGGA GAATCTGCTG CTGCAGCAGC AACAGACTGA GCAGCAGGCA
    CTGCAGCAGC GCCAGCGCCA GGAGCAGCTG CGTCTGCAGC AGTTTCAGGA GGCGCAGCGC
    CAGGCGATCC TGGACCTGTG CGTGAAACAG AGAGAGCCCG CGGAGCAGAC ATCACCCACC
    CTTGCCAACC AAACGCCGAC GTTCCCACCG CTTGCCAGCA ATCAGATCAT GCCAAGCTTA
    AATGCCCGCC CTTGCCAAAG CCAAAGCCCA AACCACAACG AATTTCAGTT GCAGCCGCAG
    CCACCACATC CCGCCCAGAA ACCGACGAAT CCCGAACCAC CGTCCGCTGT GGAACAGCAG
    CTGCACCGTC CGGTCCTAAT CCTGCCGCCC GTGGTGCTCC TGGGTGGTCG GGAGGAGGAC
    CGTTCGGTGC CACCATCGCC ACTAGTCGAG TATCCCGAGG ACGACGACGA GTATCTGTAT
    CGTGGTAGCA ACGAAAACCT GGGCACCAGG CTGCACTCCA GCCACAGTTC CAGTGCCGGC
    GATCGTTTCT GTGTGGAGGC CCGTATTAGT CTTGTACATA TAACCCTCGA ACCGATCAAT
    GCCAGCCGGA CGACCAGTTG CCTGATCGAG GAGGTAGCCG AGCCGGATTC ACAGCCGGAG
    ATTAAGCCGG TGGCAGAAGC GCAGTCTGCC AAAGTATCCG AGGCTTGTGT CGAAAGTATT
    CTCCCCGAGA CAGTTGAAAA GTTAGAAACA GCAGACCAGG TCCAGCAGGT TATACCACAG
    TTGGGGAAGC TTTACGTGGG CAGTAGCCAG CAGCAGTATG CGCAGCAGTC ATCGCCCATC
    ATCCAGGAGC CAGCTACTCC TACTATTTAC GGAAACAGCG CCGCAGCCGG AGCGCCGCAG
    TTCCCGCAGC CCGCCCAAAG GCAAGAGAAG CAGCCTCCGC AGCAGCAGCC CATCTACGCT
    AACCAGTATG AGCTGAATGT GGCCAAGGCG GCAGCTGCAG CATCTGTTTA CTCACCCAGC
    TCCTCCACCA CCAGCAACTC CAATCAACAG CAGCAGCAGC AGCGCCGGAA TGTGGCCCGT
    GGACTGCAGT ATCGTGAATC TGGAGGACTC GAGACTGGAC GTGCTGGCAA GCAGCCACCC
    AATGCGGGCA TGTTGTCAAT GGACAACTTC CGTTTGCTAA GCGTTCTTGG TCGCGGCCAC
    TTTGGCAAGG TGATTCTGTC CCAATTGCGA AGCAACAACC AGTACTACGC TATTAAGGCA
    CTGAAGAAGG GAGACATCAT TGCCCGCGAC GAAGTGGAGT CCCTGCTTAG CGAAAAGCGC
    ATCTTCGAGG TGGCCAACGC CATGCGCCAT CCGTTCTTAG TTAACTTGTA TTCGTGCTTC
    CAGACTGAGC AACACGTATG CTTTGTGATG GAATACGCTG CTGGCGGAGA TTTGATGATG
    CACATCCACA CGGACGTGTT CCTAGAGCCG AGAGCCGTTT TCTACGCCGC TTGTGTGGTT
    CTGGGCCTGC AGTACCTGCA CGAGAACAAG ATCATCTACC GGGACCTGAA GCTGGACAAT
    TTGCTTTTGG ACACGGAAGG ATATGTGAAA ATTGCGGACT TTGGTTTGTG CAAGGAGGGC
    ATGGGCTTTG GTGATCGCAC GGGCACTTTC TGTGGTACGC CCGAGTTTCT GGCACCGGAA
    GTGCTCACGG AAACTTCCTA CACACGAGCT GTGGATTGGT GGGGCTTGGG TGTGTTGATC
    TTTGAGATGT TGGTTGGTGA GTCCCCATTC CCTGGTGACG ATGAGGAGGA AGTATTCGAT
    TCAATTGTCA ACGATGAGGT GCGCTATCCG CGCTTCCTAT CGCTGGAGGC CATAGCCGTG
    ATGCGTAGGC TTTTGCGCAA GAATCCAGAG AGACGTCTGG GATCTTCGGA ACGGGATGCG
    GAGGATGTTA AGAAACAGGC ATTCTTCCGG TCAATTGTGT GGGATGACCT GCTCCTGCGA
    AAGGTTAAAC CACCATTTGT GCCGACAATT AACCACTTGG AGGATGTGTC AAACTTTGAC
    GAGGAGTTCA CGTCGGAGAA GGCTCAGCTT ACGCCACCGA AGGAGCCGCG ACACTTGACC
    GAGGAGGAGC AGTTGCTCTT CCAGGACTTT TCATACACGG CCGAATGGTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286241.1
    CDS421..4854
    Translation

    Target ORF information:

    RefSeq Version NM_001299312.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster protein kinase N, transcript variant N (Pkn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCGGATT CGTATTATCA GGGCGAATAC ATCAAGCATC CCGTTCTGTA CGAGCTCAGT 
    CACAAATATG GTTTCACAGA GAATCTGCCG GAGAGCTGTA TGTCCATACG GCTGGAGGAG
    ATCAAGGAGG CCATTCGGCG AGAGATCCGC AAGGAGCTGA AGATCAAAGA GGGCGCCGAG
    AAGCTCCGCG AGGTGGCTAA AGATCGACGA TCCCTCAGCG ATGTGGCCGT TCTTGTCAAG
    AAGAGCAAAA GTAAACTTGC CGAGCTGAAG TCCGAGTTGC AGGAGCTCGA GAGTCAAATC
    CTCCTGACAT CGGCCAACAC CGCCGTCAAT AGTAATGGAC AAGAATCGAT CACTGCCTGC
    ATTGATCCCA ATGGCGGCTT CTTGGTCAGC GGTGCAGTTG GTGGCTTGGG CGGCGGAAAC
    ACGGCTCTGG AGGGCGGCGC ACCGGCCACT GCCAATGACA AAGTGCTCGC CTCGCTGGAG
    AAGCAGCTGC AGATCGAGAT GAAGGTGAAG ACCGGGGCGG AAAACATGAT CCAGTCGCTG
    GGCATCGGAT GCGACAAGAA GCTGCTGGCG GAAGCCCACC AGATGCTGGC CGACTCGAAG
    GCCAAGATTG AGTTTTTGCG CCTGCGCATC ATCAAGGTGA AACAGAACCG CGAGCAGGCC
    GATCGCCTCA AGGCCTCGCG CCAGATGATC GACGAGCATG GCCAGACGAT CGGCGGGAAC
    AACAGCAGCC AGCCGCAGAG CCTGGAGACG ACGCTGGAGG AGCGAATCGA GGAGCTCCGC
    CATCGACTGC GAATCGAGGC AGCCGTCGTC GATGGAGCCA AGAATGTTAT ACGCACGCTG
    CAGACGGCGA ATAGGGCACC GGACAAGAAG GCTTTGCAGG AGGCCCATGG ACGTTTGTCG
    GAATCGTCGC GAAAACTAGA TCTCTTGCGC TACTCCTTGG ATTTACGACG CCAGGAGCTG
    CCCGCCGATT CGCCCGCCGC CCAGCAATTA AAAACGGAGC TGCAGATCGT CCAGCTATCG
    ACCTCCCCAG CTCCGGTCAC CTACACGTCA CTGCAGTCCG GACAAGCGGG CATACTCGGC
    GGAAAGCCGT ACCAGTCGGT ATCCTCGCTT GGACGCTGTG CCAGTGTCAC CGGAAAACTG
    GAGGTTCGCT TGCTGGGCTG TCAAGATTTG CTGGAAGATG TGCCCGGTAG ATCGAGGAGG
    GACAAGGACA ACAACTCAAG TCCGGGTGAT TTACGAAGCT TCGTCAAGGG CGTCACGTCG
    CGCAGCAGTT CAAAGAGCTA TTCGGTAAAG GATGAGACGT CCATTGAGAT CATGGCGGTC
    ATCAAGCTGG ACAATATCAC CGTTGGCCAG ACATCGTGGA AGCAATGCTC GCAGCAGGCC
    TGGGATCAGC GCTTCTCCAT CGATCTAGAC CGGTCGCGCG AACTGGAGAT TGGAGTTTAC
    TGGCGCGACT GGCGATCCCT GTGCGCCGTA AAGGTGCTGC GCCTTGAAGA GTTCATCGAC
    GATGTACGAC ATGGCATGGC ATTGCAGCTG GAGCCACAAG GTCTGCTATT TGCGGAGGTC
    AAGTTCTTGA ACCCCATGAT TTCACAGAAG CCAAAGCTGA GGCGCCAGCG TATGATCTTC
    AACAGGCAGC AAGCGAAGAA CATCTCGCGG GCCAAGCAGA TGAACATTAA TGTGGCCACA
    TGGGGCCGCC TGCTTAAGCG GAATGCTCCT AACCATGTGC ACATGGGATC GGCAGGATCA
    GGATCTTCTC TAACCGGTAG CTCGCCTATG GTGGTTGGTG GGTCTCGCGA CTCTGAGTCT
    CCGATTTCGA GGACTCCCTC ATCCGACGCA CTGGTGGAAC CGGAGCCCTA CACGCCCGGA
    GAGCAGGCAC AGAACTTGGA ATTCGATCCC GATGCAGGAA TTAATGAACA CGTGGAGACG
    CCAGGTGAAT ACCCGGATCC GGCGGCCAGT GGTCTGAGCG GAATGCGACC CCTGTCCATG
    CACATGCAGG GAATTAGTGT GCTGCCCCCG GAATCGCCAC CCGTGGCCAC CGGAGCAGCT
    GGTCGGCCCA ATACGCTCAG CTTACAGATG CCGGGTGCCA GTAAAGGACA GGTGATCCAA
    GGCGGACGGA CTGCGGCGCC TACAACGGCG CCACCGCCAC CACCTGTGCT CAAGGCGACA
    TCCACCACTC CGATATTGGA TCAGGAGCTG CAGGATGCCC TGCACGAATT CGATTTCCTG
    TCGGACCTGG ACTCACGTCC CACCACGCTG CGTCGCCTGC TGAAGGAGCA GAAGATGGCG
    CTGGATGCCG GCGCGCTGGA GAATCTGCTG CTGCAGCAGC AACAGACTGA GCAGCAGGCA
    CTGCAGCAGC GCCAGCGCCA GGAGCAGCTG CGTCTGCAGC AGTTTCAGGA GGCGCAGCGC
    CAGGCGATCC TGGACCTGTG CGTGAAACAG AGAGAGCCCG CGGAGCAGAC ATCACCCACC
    CTTGCCAACC AAACGCCGAC GTTCCCACCG CTTGCCAGCA ATCAGATCAT GCCAAGCTTA
    AATGCCCGCC CTTGCCAAAG CCAAAGCCCA AACCACAACG AATTTCAGTT GCAGCCGCAG
    CCACCACATC CCGCCCAGAA ACCGACGAAT CCCGAACCAC CGTCCGCTGT GGAACAGCAG
    CTGCACCGTC CGGTCCTAAT CCTGCCGCCC GTGGTGCTCC TGGGTGGTCG GGAGGAGGAC
    CGTTCGGTGC CACCATCGCC ACTAGTCGAG TATCCCGAGG ACGACGACGA GTATCTGTAT
    CGTGGTAGCA ACGAAAACCT GGGCACCAGG CTGCACTCCA GCCACAGTTC CAGTGCCGGC
    GATCGTTTCT GTGTGGAGGC CCGTATTAGT CTTGTACATA TAACCCTCGA ACCGATCAAT
    GCCAGCCGGA CGACCAGTTG CCTGATCGAG GAGGTAGCCG AGCCGGATTC ACAGCCGGAG
    ATTAAGCCGG TGGCAGAAGC GCAGTCTGCC AAAGTATCCG AGGCTTGTGT CGAAAGTATT
    CTCCCCGAGA CAGTTGAAAA GTTAGAAACA GCAGACCAGG TCCAGCAGGT TATACCACAG
    TTGGGGAAGC TTTACGTGGG CAGTAGCCAG CAGCAGTATG CGCAGCAGTC ATCGCCCATC
    ATCCAGGAGC CAGCTACTCC TACTATTTAC GGAAACAGCG CCGCAGCCGG AGCGCCGCAG
    TTCCCGCAGC CCGCCCAAAG GCAAGAGAAG CAGCCTCCGC AGCAGCAGCC CATCTACGCT
    AACCAGTATG AGCTGAATGT GGCCAAGGCG GCAGCTGCAG CATCTGTTTA CTCACCCAGC
    TCCTCCACCA CCAGCAACTC CAATCAACAG CAGCAGCAGC AGCGCCGGAA TGTGGCCCGT
    GGACTGCAGT ATCGTGAATC TGGAGGACTC GAGACTGGAC GTGCTGGCAA GCAGCCACCC
    AATGCGGGCA TGTTGTCAAT GGACAACTTC CGTTTGCTAA GCGTTCTTGG TCGCGGCCAC
    TTTGGCAAGG TGATTCTGTC CCAATTGCGA AGCAACAACC AGTACTACGC TATTAAGGCA
    CTGAAGAAGG GAGACATCAT TGCCCGCGAC GAAGTGGAGT CCCTGCTTAG CGAAAAGCGC
    ATCTTCGAGG TGGCCAACGC CATGCGCCAT CCGTTCTTAG TTAACTTGTA TTCGTGCTTC
    CAGACTGAGC AACACGTATG CTTTGTGATG GAATACGCTG CTGGCGGAGA TTTGATGATG
    CACATCCACA CGGACGTGTT CCTAGAGCCG AGAGCCGTTT TCTACGCCGC TTGTGTGGTT
    CTGGGCCTGC AGTACCTGCA CGAGAACAAG ATCATCTACC GGGACCTGAA GCTGGACAAT
    TTGCTTTTGG ACACGGAAGG ATATGTGAAA ATTGCGGACT TTGGTTTGTG CAAGGAGGGC
    ATGGGCTTTG GTGATCGCAC GGGCACTTTC TGTGGTACGC CCGAGTTTCT GGCACCGGAA
    GTGCTCACGG AAACTTCCTA CACACGAGCT GTGGATTGGT GGGGCTTGGG TGTGTTGATC
    TTTGAGATGT TGGTTGGTGA GTCCCCATTC CCTGGTGACG ATGAGGAGGA AGTATTCGAT
    TCAATTGTCA ACGATGAGGT GCGCTATCCG CGCTTCCTAT CGCTGGAGGC CATAGCCGTG
    ATGCGTAGGC TTTTGCGCAA GAATCCAGAG AGACGTCTGG GATCTTCGGA ACGGGATGCG
    GAGGATGTTA AGAAACAGGC ATTCTTCCGG TCAATTGTGT GGGATGACCT GCTCCTGCGA
    AAGGTTAAAC CACCATTTGT GCCGACAATT AACCACTTGG AGGATGTGTC AAACTTTGAC
    GAGGAGTTCA CGTCGGAGAA GGCTCAGCTT ACGCCACCGA AGGAGCCGCG ACACTTGACC
    GAGGAGGAGC AGTTGCTCTT CCAGGACTTT TCATACACGG CCGAATGGTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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