RNaseZ cDNA ORF clone, Drosophila melanogaster(fruit fly)
| Gene Symbol | RNaseZ |
| Entrez Gene ID | 36086 |
| Full Name | Juvenile hormone-inducible protein 1 |
| Synonyms | CG3298,Dmel\CG3298,JHI-1,RNZ,dRNase Z,dRNaseZ,dtRNaseZ,jhI-1,tRNAse Z,tRNase Z |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Drosophila melanogaster(fruit fly) |
| Genome |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_165763.3 | NP_724916.1 | ribonuclease Z |
| Drosophila melanogaster (fruit fly) | JhI-1 | NP_724916.1 |
| Oryza sativa (rice) | Os01g0232300 | NP_001042502.1 |
| Xenopus tropicalis (tropical clawed frog) | elac2 | NP_001039086.1 |
| Homo sapiens (human) | ELAC2 | NP_060597.4 |
| Rattus norvegicus (Norway rat) | Elac2 | NP_758829.1 |
| Danio rerio (zebrafish) | elac2 | NP_001243133.1 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP009743 | XP_318827.4 |
| Schizosaccharomyces pombe (fission yeast) | trz1 | NP_593023.1 |
| Arabidopsis thaliana (thale cress) | TRZ4 | NP_188247.2 |
| Pan troglodytes (chimpanzee) | ELAC2 | NP_001009034.1 |
| Bos taurus (cattle) | ELAC2 | NP_001179994.1 |
| Caenorhabditis elegans (roundworm) | hoe-1 | NP_001023109.1 |
| Arabidopsis thaliana (thale cress) | TRZ3 | NP_175628.2 |
| Canis lupus familiaris (dog) | ELAC2 | XP_546630.2 |
| Mus musculus (house mouse) | Elac2 | NP_075968.2 |
| Gallus gallus (chicken) | ELAC2 | XP_415584.4 |
Related articles in PubMed
Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The linker region was explored by Ala-scanning through two conserved regions of D. melanogaster tRNase Z: NdomTprox, located at the carboxy end of the amino domain proximal to the linker, and Tflex, a flexible site in the linker. Periodic substitutions in a hydrophobic patch (F329 and L332) at the carboxy end of NdomTprox show 2,700 and 670-fold impairment relative to wild type
Title: Substitutions in conserved regions preceding and within the linker affect activity and flexibility of tRNase ZL, the long form of tRNase Z.
The study implicates Drosophila RNase Z(L) in a novel retrograde signaling pathway initiated by the damage in mitochondria and manifested in a cell cycle delay before the mitotic entry.
Title: Knockout of Drosophila RNase ZL impairs mitochondrial transcript processing, respiration and cell cycle progression.
RNase Z(L) activity is essential for all phases of fly development that involve cell division, including growth of adult tissue progenitors during larval and metamorphic stages, and gametogenesis in adults.
Title: Developmental roles of Drosophila tRNA processing endonuclease RNase ZL as revealed with a conditional rescue system.
The study shows that dRNaseZ is necessary for 3'-end processing of nuclear & mitochondrial tRNAs. In mitotically growing imaginal discs it is required for cell proliferation and/or viability. In endoreplicating salivary glands, it controls organ size.
Title: RNAi knockdown of dRNaseZ, the Drosophila homolog of ELAC2, impairs growth of mitotic and endoreplicating tissues.
Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing
Title: Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing.
The following RNaseZ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNaseZ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OFa09509 | |
| Clone ID Related Accession (Same CDS sequence) | NM_165763.3 | |
| Accession Version | NM_165763.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-24 | |
| Organism | Drosophila melanogaster(fruit fly) | |
| Product | ribonuclease Z | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165763.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## | |
1 | ATGTATTTAG TAAAATCAGC CAGGTCGCCG ATCTACAGAA CCTTGCGAAC GCTCACAACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_724916.1 |
| CDS | 132..2432 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_165763.3 |
ORF Insert Sequence:
1 | ATGTATTTAG TAAAATCAGC CAGGTCGCCG ATCTACAGAA CCTTGCGAAC GCTCACAACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data. |
|
Gene Model Annotations for Drosophila melanogaster: The Rule-Benders. |
|
Sequence finishing and mapping of Drosophila melanogaster heterochromatin. |
|
The Release 5.1 annotation of Drosophila melanogaster heterochromatin. |
|
Combined evidence annotation of transposable elements in genome sequences. |