Dh44-R1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dh44-R1
Entrez Gene ID	36601
Full Name	Diuretic hormone 44 receptor 1
Synonyms	CG8422,DH-R,DH44-R1,DH[[44]]-R1,Dh44R1,DmeCG8422,Dmel\CG8422,anon-WO0170980.100,anon-WO0170980.101
General protein information	Preferred Names Diuretic hormone 44 receptor 1 Names CG8422 gene product from transcript CG8422-RA CG8422-PA CRF-like diuretic hormone receptor 1 DH44 receptor DH44-receptor DH[[44]] receptor Dh44-R1-PA diuretic hormone 44 receptor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50F8-50F9

mRNA and Protein(s)

mRNA	Protein	Name
NM_137116.3	NP_610960.1	diuretic hormone 44 receptor 1

Pathways from BioSystems

Homologies

	CRHR2	XP_001085987.2
Mus musculus (house mouse)	Crhr2	NP_034083.2
Gallus gallus (chicken)	CRHR2	NP_989785.1
Xenopus tropicalis (tropical clawed frog)	crhr2	XP_004915648.1
Homo sapiens (human)	CRHR2	NP_001189411.1
Pan troglodytes (chimpanzee)	CRHR2	XP_003318285.1
Rattus norvegicus (Norway rat)	Crhr2	NP_073205.1
Canis lupus familiaris (dog)	CRHR2	XP_005628743.1
Bos taurus (cattle)	CRHR2	NP_001179474.1
Danio rerio (zebrafish)	LOC100335005	XP_002667894.2
Drosophila melanogaster (fruit fly)	Dh44-R1	NP_610960.1
Anopheles gambiae (African malaria mosquito)	GPRDIH2	XP_315468.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Dh44/DH44 receptor system directs the detection and consumption of nutritive sugars through a positive feedback loop.
Title: Nutrient Sensor in the Brain Directs the Action of the Brain-Gut Axis in Drosophila.

Fundamental differences in signaling, association with the arrestins, and peptide sensitivity between CG8422 and CG12370 are discussed.
Title: Functional differences between two CRF-related diuretic hormone receptors in Drosophila.

The following Dh44-R1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dh44-R1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09976	NM_137116.3 Latest version!	Drosophila melanogaster diuretic hormone 44 receptor 1 (Dh44-R1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dh44-R1 ( NM_137116.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610960.1 Drosophila melanogaster diuretic hormone 44 receptor 1 (Dh44-R1), mRNA.

Price & Availability↑

CloneID	OFa09976
Clone ID Related Accession (Same CDS sequence)	NM_137116.3
Accession Version	NM_137116.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diuretic hormone 44 receptor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137116.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGACC ACAACCACAT CGATTCGGTG AACGCCAGCG GGAGTGATCC GCTCCTGGAT 
CTGCACAACC TAGATGGCAT CGGTGAGAGC GTGGAGTTGC AGTGCCTGGT GCAGGAGCAC 
ATCGAGGCAT CCACCTATGG CAACGATAGT GGCCACTGCC TCACCCAGTT CGACTCGATC 
CTGTGCTGGC CGAGGACAGC TCGCGGTACC CTGGCGGTGC TCCAGTGCAT GGACGAGTTG 
CAGGGAATTC ATTACGATAG CAGCAAGAAT GCAACGAGGT TCTGCCACGC CAATGGAACT 
TGGGAGAAGT ACACCAACTA CGATGCCTGC GCCCACCTGC CCGCCCCAGA ATCCGTGCCG 
GAGTTCGAGG TCATCGTGGA GCTGCCCACC ATTATCTACT ACATTGGATA CACCCTCAGC 
CTGGTATCGC TCTCGCTGGC GCTGATTGTT TTCGCCTACT TCAAGGAGCT GCGTTGCCTG 
CGCAACACCA TCCACGCCAA CTTGTTCTTC ACGTACATCA TGTCGGCTTT GTTCTGGATA 
CTCTTGTTAT CCGTCCAGAT CTCCATCCGA AGTGGAGTGG GCAGCTGCAT TGCTCTGATC 
ACCCTGTTCC ACTTCTTCAC CCTGACCAAC TTCTTTTGGA TGCTGGTCGA GGGTTTGTAC 
CTGTACATGC TGGTGGTTAA GACTTTCTCC GGGGACAATT TACGCTTTAA TATCTACGCC 
TCCATAGGCT GGGGTGGCCC GGCCTTGTTT GTTGTCACCT GGGCTGTGGC CAAGAGTCTG 
ACGGTCACCT ACAGCACCCC CGAAAAGTAC GAAATCAACT GCCCCTGGAT GCAGGAGACC 
CATGTGGATT GGATATACCA GGGACCTGTC TGTGCGGTGC TAATAATCAA TCTCACATTT 
CTGCTGCGTA TCATGTGGGT TCTAATCACA AAGCTGCGCT CGGCCAATAC AGTGGAGACT 
CGTCAGTATA GGAAAGCTGC TAAGGCACTG CTGGTGCTAA TCCCACTTTT TGGCATCACC 
TACCTGGTGG TGCTGGCTGG TCCCTCGGAA TCTGGCCTAA TGGGACACAT GTTTGCCGTC 
CTGCGAGCGG TATTACTCAG CACTCAGGGA TTCTCGGTGT CGCTGTTCTA CTGTTTCCTC 
AACTCGGAGG TGCGAAATGC ACTGAGACAC CACATTTCCA CGTGGCGGGA CACGCGCACC 
ATTCAGCTTA ACCAGAACCG ACGTTATACC ACGAAAAGCT TCTCGAAAGG CGGTGGTTCC 
CCCCGAGCCG AGAGTATGCG ACCTTTGACC AGCTACTATG GGCGTGGCAA GCGGGAATCG 
TGCGTAAGTT CGGCCACCAC CACCACGCTG GTGGGTCAGC ATGCCCCACT TTCACTCCAC 
CGGGGCTCCA ACAATGCCCT GCACACGATG CCCACTTTGG CGGCAAATGC CATGAGTTCC 
GGCAGCACTC TGAGCGTAAT GCCCCGGGCC ATTAGTCCCC TGATGAGGCA AGGACTCGAG 
GAGAACTCGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610960.1
CDS	603..2117
Translation	MSDHNHIDSVNASGSDPLLDLHNLDGIGESVELQCLVQEHIEASTYGNDSGHCLTQFDSILCWPRTARGTLAVLQCMDELQGIHYDSSKNATRFCHANGTWEKYTNYDACAHLPAPESVPEFEVIVELPTIIYYIGYTLSLVSLSLALIVFAYFKELRCLRNTIHANLFFTYIMSALFWILLLSVQISIRSGVGSCIALITLFHFFTLTNFFWMLVEGLYLYMLVVKTFSGDNLRFNIYASIGWGGPALFVVTWAVAKSLTVTYSTPEKYEINCPWMQETHVDWIYQGPVCAVLIINLTFLLRIMWVLITKLRSANTVETRQYRKAAKALLVLIPLFGITYLVVLAGPSESGLMGHMFAVLRAVLLSTQGFSVSLFYCFLNSEVRNALRHHISTWRDTRTIQLNQNRRYTTKSFSKGGGSPRAESMRPLTSYYGRGKRESCVSSATTTTLVGQHAPLSLHRGSNNALHTMPTLAANAMSSGSTLSVMPRAISPLMRQGLEENSV

Target ORF information:

RefSeq Version	NM_137116.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diuretic hormone 44 receptor 1 (Dh44-R1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137116.3

ORF Insert Sequence:

ATGAGTGACC ACAACCACAT CGATTCGGTG AACGCCAGCG GGAGTGATCC GCTCCTGGAT 
CTGCACAACC TAGATGGCAT CGGTGAGAGC GTGGAGTTGC AGTGCCTGGT GCAGGAGCAC 
ATCGAGGCAT CCACCTATGG CAACGATAGT GGCCACTGCC TCACCCAGTT CGACTCGATC 
CTGTGCTGGC CGAGGACAGC TCGCGGTACC CTGGCGGTGC TCCAGTGCAT GGACGAGTTG 
CAGGGAATTC ATTACGATAG CAGCAAGAAT GCAACGAGGT TCTGCCACGC CAATGGAACT 
TGGGAGAAGT ACACCAACTA CGATGCCTGC GCCCACCTGC CCGCCCCAGA ATCCGTGCCG 
GAGTTCGAGG TCATCGTGGA GCTGCCCACC ATTATCTACT ACATTGGATA CACCCTCAGC 
CTGGTATCGC TCTCGCTGGC GCTGATTGTT TTCGCCTACT TCAAGGAGCT GCGTTGCCTG 
CGCAACACCA TCCACGCCAA CTTGTTCTTC ACGTACATCA TGTCGGCTTT GTTCTGGATA 
CTCTTGTTAT CCGTCCAGAT CTCCATCCGA AGTGGAGTGG GCAGCTGCAT TGCTCTGATC 
ACCCTGTTCC ACTTCTTCAC CCTGACCAAC TTCTTTTGGA TGCTGGTCGA GGGTTTGTAC 
CTGTACATGC TGGTGGTTAA GACTTTCTCC GGGGACAATT TACGCTTTAA TATCTACGCC 
TCCATAGGCT GGGGTGGCCC GGCCTTGTTT GTTGTCACCT GGGCTGTGGC CAAGAGTCTG 
ACGGTCACCT ACAGCACCCC CGAAAAGTAC GAAATCAACT GCCCCTGGAT GCAGGAGACC 
CATGTGGATT GGATATACCA GGGACCTGTC TGTGCGGTGC TAATAATCAA TCTCACATTT 
CTGCTGCGTA TCATGTGGGT TCTAATCACA AAGCTGCGCT CGGCCAATAC AGTGGAGACT 
CGTCAGTATA GGAAAGCTGC TAAGGCACTG CTGGTGCTAA TCCCACTTTT TGGCATCACC 
TACCTGGTGG TGCTGGCTGG TCCCTCGGAA TCTGGCCTAA TGGGACACAT GTTTGCCGTC 
CTGCGAGCGG TATTACTCAG CACTCAGGGA TTCTCGGTGT CGCTGTTCTA CTGTTTCCTC 
AACTCGGAGG TGCGAAATGC ACTGAGACAC CACATTTCCA CGTGGCGGGA CACGCGCACC 
ATTCAGCTTA ACCAGAACCG ACGTTATACC ACGAAAAGCT TCTCGAAAGG CGGTGGTTCC 
CCCCGAGCCG AGAGTATGCG ACCTTTGACC AGCTACTATG GGCGTGGCAA GCGGGAATCG 
TGCGTAAGTT CGGCCACCAC CACCACGCTG GTGGGTCAGC ATGCCCCACT TTCACTCCAC 
CGGGGCTCCA ACAATGCCCT GCACACGATG CCCACTTTGG CGGCAAATGC CATGAGTTCC 
GGCAGCACTC TGAGCGTAAT GCCCCGGGCC ATTAGTCCCC TGATGAGGCA AGGACTCGAG 
GAGAACTCGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dh44-R1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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