Pepck1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pepck1
Entrez Gene ID	37131
Full Name	Phosphoenolpyruvate carboxykinase
Synonyms	143299_at,CG10924,CG17725,Dmel\CG17725,Dromel_CG17725_FBtr0086701_pepck_mORF,PEPCK,dPEPCK,pepck,pepck1
General protein information	Preferred Names Phosphoenolpyruvate carboxykinase Names CG17725 gene product from transcript CG17725-RA CG17725-PA Pepck-PA Phosphoenolpyruvate-carboxykinase phosphoenol pyruvate carboxykinase phosphoenolpyruvate carboxy kinase phosphoenolpyruvate carboxykinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55D3-55D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079060.3	NP_523784.2	phosphoenolpyruvate carboxykinase 1

Pathways from BioSystems

Homologies

	PCK2	XP_001111487.1
Canis lupus familiaris (dog)	PCK2	XP_537379.2
Mus musculus (house mouse)	Pck2	NP_083270.1
Rattus norvegicus (Norway rat)	Pck2	NP_001101847.2
Homo sapiens (human)	PCK2	NP_004554.2
Drosophila melanogaster (fruit fly)	Pepck	NP_523784.2
Caenorhabditis elegans (roundworm)	pck-1	NP_497134.1
Pan troglodytes (chimpanzee)	PCK2	XP_001165863.1
Bos taurus (cattle)	PCK2	NP_001192523.1
Danio rerio (zebrafish)	pck2	NP_998357.1
Xenopus tropicalis (tropical clawed frog)	pck2	NP_001034816.1
Gallus gallus (chicken)	PCK2	NP_990801.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003350	XP_003436452.1
Caenorhabditis elegans (roundworm)	pck-2	NP_001021587.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pepck1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pepck1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10501	NM_079060.3 Latest version!	Drosophila melanogaster phosphoenolpyruvate carboxykinase 1 (Pepck1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pepck1 ( NM_079060.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523784.2 Drosophila melanogaster phosphoenolpyruvate carboxykinase 1 (Pepck1), mRNA.

Price & Availability↑

CloneID	OFa10501
Clone ID Related Accession (Same CDS sequence)	NM_079060.3
Accession Version	NM_079060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphoenolpyruvate carboxykinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079060.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTGAGC TCATTGAACA AAGCAAAATT ATCTCCGGCA ATGTCTGCGG TCTGCCCCAG 
TTGCACAAGC TGCGCCAGGA CAATTGCGGT CTGTACAGCC ACATCCGTGG CATTCCCATC 
TCCTATGGAA ATGTGGATTT GCTGACCACC GGTGTCCGTG CCTTCGTGGA GGAGGGCATC 
GCCCTCTGCC AGCCCGACCA GGTGCACATC TGCGATGGCA GCGAGCAGGA GAACAAGGTG 
CTCATCAAGA GCCTCTTGGA GGCTGGCACC ATTGTGCCGC TGCCCAAGTA CGACAATTGC 
TGGCTGGCCC GCACCAATCC GGCGGATGTG GCCCGCGTCG AGTCGCGCAC TTTCATCTGC 
ACCGAGCGGC GGGAGGAGAC GATTCCCACT CCAGTGGAGG GTGTCAAGGG AACCCTGGGC 
AACTGGATCT CGCCCAGCGA CATGGATGCT GCAGTGCAGC AGCGATTCCC CGGCTGCATG 
AAGGGTCGCA CCATGTACGT GGTGCCCTTC AGCATGGGTC CTGTGGGCTC CCCGCTCTCT 
AAGATCGGAA TCGAACTGAC GGACTCTGCT TACGTGGTGG CCTCCATGAG GATCATGACA 
CGCATGGGAG CCGCTGTCTT GCGCCAGCTG GCCAAGAAGG AGGAATTCGT CCGCGCCCTA 
CACTCTGTGG GCGCCCCTGC CAACGGACAG GTGGAGCAGC CATCCTGGCC TTGTGATCCC 
GAACGCACCA TCATCCTGCA CAAGCCCGCC GAGAACCTTA TTGTGTCTTA CGGATCGGGC 
TACGGCGGCA ACTCGCTGCT GGGCAAGAAG TGCTTCGCCC TGAGGATTGG CAGCACCATT 
GCCAAGCAGG AGGGCTGGCT GGCCGAGCAC ATGTTGATTC TGGGCATCAC CGATCCCAAG 
GGCGAGAAGA AGTACATCAC TGCCGCCTTT CCCTCGGCCT GTGGCAAGAC CAATCTGGCC 
ATGCTGAATC CCTCGTTGGC CAACTACAAG GTGGAATGCG TGGGCGACGA CATTGCGTGG 
ATGAAGTTTG ACTCGCAGGG AGTGCTGCGT GCCATCAACC CAGAGAATGG ATTCTTTGGT 
GTGGCTCCAG GAACCTCAAT GGAGACCAAT CCCATTGCCA TGAACACTGT TTTCAAGAAC 
ACCATCTTCA CGAACGTGGC CTCCACCTCT GATGGCGGTG TGTTCTGGGA GGGCATGGAA 
AGCAGTCTGG CTCCCAATGT CCAAATCACT GACTGGTTGG GCAAACCCTG GACCAAGGAT 
TCCGGCAAGC CCGCTGCGCA TCCCAACTCC CGCTTCTGCA CTCCAGCCGC CCAGTGCCCG 
ATTATCGATG AAGCCTGGGA AGATCCGGCG GGAGTGCCCA TCTCCGCCAT GCTCTTCGGA 
GGACGTCGTC CCGCCGGAGT GCCCCTAATC TATGAGGCTC GGGACTGGAC CCACGGAGTC 
TTCATTGGAG CAGCCATGAG GAGTGAGGCC ACTGCTGCGG CCGAGCACAA GGGCAAGGTG 
ATCATGCACG ATCCGTTCGC CATGAGGCCC TTCTTTGGCT ACAACTTTGG CGACTACGTG 
GCCCACTGGC TGAGCATGGA GAAGCGTGGT CAGGTGCCAA AGATCTTCCA CGTCAACTGG 
TTCCGCAAGA GTGCCGAGGG CAAGTTCATG TGGCCCGGAT ACGGAGAGAA CTCCCGTGTG 
CTGGAATGGA TCCTGCGCAG GGTCAATGGC GAATCCTGCT ACGTGGACTC GGCCATTGGA 
CACATTCCCG CCGAGGGTGC CCTAAACCTC GATGGCATGA AGGATAAGGT GGACGTGAAG 
GAGATCTTCT CGCTGCCCAA GGAGTTCTGG TCGCAGGAGG TCAAGGATAT TCGCACCTAC 
TTCGAGTCAC AGGTGGGCGC CGATCTTCCG GCCAGCATTT ACCAGCAGCT GGACGAGCTA 
TCTTCCCGCG TAGACAACCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523784.2
CDS	272..2215
Translation	MPELIEQSKIISGNVCGLPQLHKLRQDNCGLYSHIRGIPISYGNVDLLTTGVRAFVEEGIALCQPDQVHICDGSEQENKVLIKSLLEAGTIVPLPKYDNCWLARTNPADVARVESRTFICTERREETIPTPVEGVKGTLGNWISPSDMDAAVQQRFPGCMKGRTMYVVPFSMGPVGSPLSKIGIELTDSAYVVASMRIMTRMGAAVLRQLAKKEEFVRALHSVGAPANGQVEQPSWPCDPERTIILHKPAENLIVSYGSGYGGNSLLGKKCFALRIGSTIAKQEGWLAEHMLILGITDPKGEKKYITAAFPSACGKTNLAMLNPSLANYKVECVGDDIAWMKFDSQGVLRAINPENGFFGVAPGTSMETNPIAMNTVFKNTIFTNVASTSDGGVFWEGMESSLAPNVQITDWLGKPWTKDSGKPAAHPNSRFCTPAAQCPIIDEAWEDPAGVPISAMLFGGRRPAGVPLIYEARDWTHGVFIGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGDYVAHWLSMEKRGQVPKIFHVNWFRKSAEGKFMWPGYGENSRVLEWILRRVNGESCYVDSAIGHIPAEGALNLDGMKDKVDVKEIFSLPKEFWSQEVKDIRTYFESQVGADLPASIYQQLDELSSRVDNL

Target ORF information:

RefSeq Version	NM_079060.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphoenolpyruvate carboxykinase 1 (Pepck1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079060.3

ORF Insert Sequence:

ATGCCTGAGC TCATTGAACA AAGCAAAATT ATCTCCGGCA ATGTCTGCGG TCTGCCCCAG 
TTGCACAAGC TGCGCCAGGA CAATTGCGGT CTGTACAGCC ACATCCGTGG CATTCCCATC 
TCCTATGGAA ATGTGGATTT GCTGACCACC GGTGTCCGTG CCTTCGTGGA GGAGGGCATC 
GCCCTCTGCC AGCCCGACCA GGTGCACATC TGCGATGGCA GCGAGCAGGA GAACAAGGTG 
CTCATCAAGA GCCTCTTGGA GGCTGGCACC ATTGTGCCGC TGCCCAAGTA CGACAATTGC 
TGGCTGGCCC GCACCAATCC GGCGGATGTG GCCCGCGTCG AGTCGCGCAC TTTCATCTGC 
ACCGAGCGGC GGGAGGAGAC GATTCCCACT CCAGTGGAGG GTGTCAAGGG AACCCTGGGC 
AACTGGATCT CGCCCAGCGA CATGGATGCT GCAGTGCAGC AGCGATTCCC CGGCTGCATG 
AAGGGTCGCA CCATGTACGT GGTGCCCTTC AGCATGGGTC CTGTGGGCTC CCCGCTCTCT 
AAGATCGGAA TCGAACTGAC GGACTCTGCT TACGTGGTGG CCTCCATGAG GATCATGACA 
CGCATGGGAG CCGCTGTCTT GCGCCAGCTG GCCAAGAAGG AGGAATTCGT CCGCGCCCTA 
CACTCTGTGG GCGCCCCTGC CAACGGACAG GTGGAGCAGC CATCCTGGCC TTGTGATCCC 
GAACGCACCA TCATCCTGCA CAAGCCCGCC GAGAACCTTA TTGTGTCTTA CGGATCGGGC 
TACGGCGGCA ACTCGCTGCT GGGCAAGAAG TGCTTCGCCC TGAGGATTGG CAGCACCATT 
GCCAAGCAGG AGGGCTGGCT GGCCGAGCAC ATGTTGATTC TGGGCATCAC CGATCCCAAG 
GGCGAGAAGA AGTACATCAC TGCCGCCTTT CCCTCGGCCT GTGGCAAGAC CAATCTGGCC 
ATGCTGAATC CCTCGTTGGC CAACTACAAG GTGGAATGCG TGGGCGACGA CATTGCGTGG 
ATGAAGTTTG ACTCGCAGGG AGTGCTGCGT GCCATCAACC CAGAGAATGG ATTCTTTGGT 
GTGGCTCCAG GAACCTCAAT GGAGACCAAT CCCATTGCCA TGAACACTGT TTTCAAGAAC 
ACCATCTTCA CGAACGTGGC CTCCACCTCT GATGGCGGTG TGTTCTGGGA GGGCATGGAA 
AGCAGTCTGG CTCCCAATGT CCAAATCACT GACTGGTTGG GCAAACCCTG GACCAAGGAT 
TCCGGCAAGC CCGCTGCGCA TCCCAACTCC CGCTTCTGCA CTCCAGCCGC CCAGTGCCCG 
ATTATCGATG AAGCCTGGGA AGATCCGGCG GGAGTGCCCA TCTCCGCCAT GCTCTTCGGA 
GGACGTCGTC CCGCCGGAGT GCCCCTAATC TATGAGGCTC GGGACTGGAC CCACGGAGTC 
TTCATTGGAG CAGCCATGAG GAGTGAGGCC ACTGCTGCGG CCGAGCACAA GGGCAAGGTG 
ATCATGCACG ATCCGTTCGC CATGAGGCCC TTCTTTGGCT ACAACTTTGG CGACTACGTG 
GCCCACTGGC TGAGCATGGA GAAGCGTGGT CAGGTGCCAA AGATCTTCCA CGTCAACTGG 
TTCCGCAAGA GTGCCGAGGG CAAGTTCATG TGGCCCGGAT ACGGAGAGAA CTCCCGTGTG 
CTGGAATGGA TCCTGCGCAG GGTCAATGGC GAATCCTGCT ACGTGGACTC GGCCATTGGA 
CACATTCCCG CCGAGGGTGC CCTAAACCTC GATGGCATGA AGGATAAGGT GGACGTGAAG 
GAGATCTTCT CGCTGCCCAA GGAGTTCTGG TCGCAGGAGG TCAAGGATAT TCGCACCTAC 
TTCGAGTCAC AGGTGGGCGC CGATCTTCCG GCCAGCATTT ACCAGCAGCT GGACGAGCTA 
TCTTCCCGCG TAGACAACCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pepck1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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