Ppat-Dpck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppat-Dpck
Entrez Gene ID	38647
Full Name	Bifunctional Phosphopantetheine adenylyltransferase - Dephospho-CoA kinase
Synonyms	CG10575,DPCK/PPAT,Dmel\CG10575,PPAT-DPCK,dCOASY,dPPAT-DPCK
General protein information	Preferred Names Bifunctional Phosphopantetheine adenylyltransferase - Dephospho-CoA kinase Names CG10575 gene product from transcript CG10575-RA 4'-phosphopantetheine adenylyltransferase - dephospho-CoA kinase CG10575-PA Ppat-Dpck-PA bifunctional phosphopantetheine adenylyltransferase - Dephospho-CoA kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64E7-64E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_139728.2	NP_647985.1	bifunctional phosphopantetheine adenylyltransferase - Dephospho-CoA kinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	COASY	NP_001035997.2
Danio rerio (zebrafish)	coasy	NP_001004577.1
Arabidopsis thaliana (thale cress)	COAD	NP_179417.1
Canis lupus familiaris (dog)	COASY	XP_005624507.1
Drosophila melanogaster (fruit fly)	Ppat-Dpck	NP_647985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005460	XP_315463.3
Oryza sativa (rice)	Os07g0179400	NP_001059035.1
Mus musculus (house mouse)	Coasy	NP_082172.2
Pan troglodytes (chimpanzee)	COASY	XP_001163322.2
	COASY	XP_001110129.2
Bos taurus (cattle)	COASY	XP_005220858.1
Rattus norvegicus (Norway rat)	Coasy	NP_001006955.1
Caenorhabditis elegans (roundworm)	Y65B4A.8	NP_490766.2
Xenopus tropicalis (tropical clawed frog)	coasy	XP_002942317.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppat-Dpck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppat-Dpck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17621	NM_139728.2 Latest version!	Drosophila melanogaster bifunctional phosphopantetheine adenylyltransferase - Dephospho-CoA kinase (Ppat-Dpck), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppat-Dpck ( NM_139728.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647985.1 Drosophila melanogaster bifunctional phosphopantetheine adenylyltransferase - Dephospho-CoA kinase (Ppat-Dpck), mRNA.

Price & Availability↑

CloneID	OFa17621
Clone ID Related Accession (Same CDS sequence)	NM_139728.2
Accession Version	NM_139728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifunctional phosphopantetheine adenylyltransferase - Dephospho-CoA kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_139728.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTCAT CCACAGGTCT CCTGGTGGTG TCCAACATCA AGCACCTTGG CAAATCCCTG 
CGAGCCATCG AGAAGTACGT GAATTCACTG TACATCCACC TAAATGTGGC GGGGTCAACG 
TCCACGACGT CACCAGTTCC ACCGCCTCCG GTTTGGGGTC GTCTAATCTC GCAGCTGTAC 
GCCAACAGCA GCAGCTATGT GGGCAAGCAG TTGGACCTTC GCGTCCTTGT CTCTCCCCTA 
CGACCAGGTG CCAATGGATC CCTGAAGTTG CGCCAGCCCG TCGACCTAAT CTTCTCGGAT 
GCACATCATC CGGAGCTGTG CGACAGGCTT CGCGCGGATC TTAACATCAG CAAGCCAACA 
ATCTTCCTGG ATGACTCGGT CATCTCGGAT TTAAGTGCCC AGCAGGATGA CACCCAGCCG 
CCTAAGGTGT ATCCCTCGGT TGTCCTGGGC GGAACATTCG ATCGCATCCA TCTGGGACAC 
AAGATATTCC TCACCCAGGC TGTACTGCGC ACCTGCAAGC GTTTGGTTGT GGGCGTAACC 
ACCTCCGCCA TGACGAAGGG AAAGACGCTG CCGGACTTGA TTTTGCCCGT GGAAGAGCGC 
ATCGCCCGGC TAAGGGAGTT CCTGGTGGAC ATAGATGATA CGCTGCAGTA CGAAATTGTG 
CCCATCGATG ATCCCTTTGG TCCCACGCAA GTGGATCCTG ACCTGGACAT GATTGTGGTC 
AGTGCGGAGA CGTTGCGAGG AGGGCAGAAG GTCAACGAGG TACGCTCCGC TAAGCAACTG 
CGCGAGCTGG AGATCTTTGT GATTGACATT GTTGAAAGCA ACGTGCATGA TGGCATCCAC 
GAGACCAAGG TCAGCTCGAG TAACACACGC ATCGATCTGC TGGGAACCCG CTGGAGAAGG 
CCGGAGCCAC GACCACAGCT CCCGCCGCGC CCTTACATTA TTGGACTCAC TGGCGGCATC 
GCATCTGGCA AGAGCAAGAT GGGCGAGAGA TTGGCCAACA TGGGCGCCCA CGTGATCGAC 
TGCGATAAGG TGGCGCACGA TGTTTACGAA CCTGGTCAGT TGTGCTACAC CCGAATTGTG 
CAGCACTTCG GACAGGGTAT TGTTTCAGAC GATGGTCGCA TCGATCGGTC CAAGCTGGGA 
CCCTTGGTGT TTGCCGATCC CAAGCAGTTG CAAGCACTCA ACGGCATTGT CTGGCCGGAA 
CTTATTGCGG AGGTTAACAG GCGGCTGGAT GCACTGCGTT CCCAGGCGGA CGTGCCGCGT 
GTGGTGGTCC TGGAGGCAGC GGTGCTGCTG CGAGCGGGCT GGGAGACCAA TTGCCATGAG 
GTGTGGTCCA TGATTGTGCC ACCGGATGAG GCTGTGCGGC GGATTATTGA GCGCAACAAG 
TTGAGCGAAG TGGAGGCCCA AAAGCGACTG GCCAGTCAGG TGCCCAATTC TGAGATCGTG 
GCCAAGTCGC ATGTGATATT CAGTTCGCAA TGGGATCACG AATTCACCCA GAAACAGGCG 
GAGCGTGCGT GGAAAATGCT TACCAAGGAA CTGGACTCTT ACCAGAGCAG CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647985.1
CDS	36..1592
Translation	MASSTGLLVVSNIKHLGKSLRAIEKYVNSLYIHLNVAGSTSTTSPVPPPPVWGRLISQLYANSSSYVGKQLDLRVLVSPLRPGANGSLKLRQPVDLIFSDAHHPELCDRLRADLNISKPTIFLDDSVISDLSAQQDDTQPPKVYPSVVLGGTFDRIHLGHKIFLTQAVLRTCKRLVVGVTTSAMTKGKTLPDLILPVEERIARLREFLVDIDDTLQYEIVPIDDPFGPTQVDPDLDMIVVSAETLRGGQKVNEVRSAKQLRELEIFVIDIVESNVHDGIHETKVSSSNTRIDLLGTRWRRPEPRPQLPPRPYIIGLTGGIASGKSKMGERLANMGAHVIDCDKVAHDVYEPGQLCYTRIVQHFGQGIVSDDGRIDRSKLGPLVFADPKQLQALNGIVWPELIAEVNRRLDALRSQADVPRVVVLEAAVLLRAGWETNCHEVWSMIVPPDEAVRRIIERNKLSEVEAQKRLASQVPNSEIVAKSHVIFSSQWDHEFTQKQAERAWKMLTKELDSYQSSL

Target ORF information:

RefSeq Version	NM_139728.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifunctional phosphopantetheine adenylyltransferase - Dephospho-CoA kinase (Ppat-Dpck), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139728.2

ORF Insert Sequence:

ATGGCTTCAT CCACAGGTCT CCTGGTGGTG TCCAACATCA AGCACCTTGG CAAATCCCTG 
CGAGCCATCG AGAAGTACGT GAATTCACTG TACATCCACC TAAATGTGGC GGGGTCAACG 
TCCACGACGT CACCAGTTCC ACCGCCTCCG GTTTGGGGTC GTCTAATCTC GCAGCTGTAC 
GCCAACAGCA GCAGCTATGT GGGCAAGCAG TTGGACCTTC GCGTCCTTGT CTCTCCCCTA 
CGACCAGGTG CCAATGGATC CCTGAAGTTG CGCCAGCCCG TCGACCTAAT CTTCTCGGAT 
GCACATCATC CGGAGCTGTG CGACAGGCTT CGCGCGGATC TTAACATCAG CAAGCCAACA 
ATCTTCCTGG ATGACTCGGT CATCTCGGAT TTAAGTGCCC AGCAGGATGA CACCCAGCCG 
CCTAAGGTGT ATCCCTCGGT TGTCCTGGGC GGAACATTCG ATCGCATCCA TCTGGGACAC 
AAGATATTCC TCACCCAGGC TGTACTGCGC ACCTGCAAGC GTTTGGTTGT GGGCGTAACC 
ACCTCCGCCA TGACGAAGGG AAAGACGCTG CCGGACTTGA TTTTGCCCGT GGAAGAGCGC 
ATCGCCCGGC TAAGGGAGTT CCTGGTGGAC ATAGATGATA CGCTGCAGTA CGAAATTGTG 
CCCATCGATG ATCCCTTTGG TCCCACGCAA GTGGATCCTG ACCTGGACAT GATTGTGGTC 
AGTGCGGAGA CGTTGCGAGG AGGGCAGAAG GTCAACGAGG TACGCTCCGC TAAGCAACTG 
CGCGAGCTGG AGATCTTTGT GATTGACATT GTTGAAAGCA ACGTGCATGA TGGCATCCAC 
GAGACCAAGG TCAGCTCGAG TAACACACGC ATCGATCTGC TGGGAACCCG CTGGAGAAGG 
CCGGAGCCAC GACCACAGCT CCCGCCGCGC CCTTACATTA TTGGACTCAC TGGCGGCATC 
GCATCTGGCA AGAGCAAGAT GGGCGAGAGA TTGGCCAACA TGGGCGCCCA CGTGATCGAC 
TGCGATAAGG TGGCGCACGA TGTTTACGAA CCTGGTCAGT TGTGCTACAC CCGAATTGTG 
CAGCACTTCG GACAGGGTAT TGTTTCAGAC GATGGTCGCA TCGATCGGTC CAAGCTGGGA 
CCCTTGGTGT TTGCCGATCC CAAGCAGTTG CAAGCACTCA ACGGCATTGT CTGGCCGGAA 
CTTATTGCGG AGGTTAACAG GCGGCTGGAT GCACTGCGTT CCCAGGCGGA CGTGCCGCGT 
GTGGTGGTCC TGGAGGCAGC GGTGCTGCTG CGAGCGGGCT GGGAGACCAA TTGCCATGAG 
GTGTGGTCCA TGATTGTGCC ACCGGATGAG GCTGTGCGGC GGATTATTGA GCGCAACAAG 
TTGAGCGAAG TGGAGGCCCA AAAGCGACTG GCCAGTCAGG TGCCCAATTC TGAGATCGTG 
GCCAAGTCGC ATGTGATATT CAGTTCGCAA TGGGATCACG AATTCACCCA GAAACAGGCG 
GAGCGTGCGT GGAAAATGCT TACCAAGGAA CTGGACTCTT ACCAGAGCAG CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ppat-Dpck cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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