Nelf-E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelf-E
Entrez Gene ID	38982
Full Name	Negative elongation factor E
Synonyms	CG5994,Dmel\CG5994,NELF,NELF-E,Nelf-e,NelfE,anon-66Da,cg5994,dNelf-E
General protein information	Preferred Names Negative elongation factor E Names CG5994 gene product from transcript CG5994-RA CG5994-PA Nelf-E-PA anonymous-66Da negative elongation factor negative elongation factor E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66D8-66D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_139984.3	NP_648241.1	negative elongation factor E

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006237	XP_316305.4
Drosophila melanogaster (fruit fly)	Nelf-E	NP_648241.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

biochemical analysis reveals that the sequence-specific transcription factor, GAF, orchestrates efficient pausing by recruiting NELF to promoters before transcription initiation and by assisting in loading NELF onto Pol II after initiation
Title: Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing.

The finding that both phenotypes of arrested embryos are obtained in embryos that lack maternally provided NELF-A as well as in embryos with reduced levels of maternal NELF-E show that these phenotypes result from the reduced activity of the NELF complex.
Title: NELF potentiates gene transcription in the Drosophila embryo.

propose that NELF and DSIF cause polymerase to pause in the promoter proximal region of hsp70
Title: NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.

The following Nelf-E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelf-E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17910	NM_139984.3 Latest version!	Drosophila melanogaster negative elongation factor E (Nelf-E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelf-E ( NM_139984.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648241.1 Drosophila melanogaster negative elongation factor E (Nelf-E), mRNA.

Price & Availability↑

CloneID	OFa17910
Clone ID Related Accession (Same CDS sequence)	NM_139984.3
Accession Version	NM_139984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	negative elongation factor E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139984.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTACA TACACTTCCC CAACAATTTA ACCGAAGAGG AGCAAATGCT GCAGGCCAAG 
TATCAGAAAC TCAAGAAAAA GAAAAAGGCA CTGCAAGCGC ACAAGGCGCC CAAGCCGGAG 
CCGGAGAGCT CCTTGACCTT GAAGCGTCCC ACGGATGCGC GAGATGCCCG TGAAGTGGCC 
AGGAAACTAA TCAAAAGCGG CGCCATTCCC GCCATCCAGA AGCAGACCAA GCAAGATCAA 
ACCTCCTTCA AGCGGCCCAA AGGACAGGAA AGAGCCAAGC GCTCCACCTC GGAAACCACT 
GTTGCCTCCT ACCAGCCCTT TTCGTCTACG CAGAACGACG TGGCCCAGGA GACGATCATT 
AGTGAGATCA TTAAGGAGGA GCCGCGTCGA CAGAATCTCT ACCAACACTT CGCCACCGAG 
CGGGATCGAG AGGAGCGCGG CATGCCCGAG AAGGTGCCCA TGGACACAGC GCAGCCAGAG 
AAGCCACGGG CCGGCAATAC CATCTTCGTG TCCGGCAACA AGGTCACCGA GGATTTCTTA 
AAAAAGACAT TCAACGACTA CGGCACCATT GTCAATGTCT CCATGGAGAT CGAAAAGAGT 
CGCGGCTTTG TTTCCTTCGC CAAACCCGAG TCGGCAGATC GCGCCATTGC GGAGATTCAT 
GGCAAGAATG TGAATGGCAT CAACTTGCAG GTGCAGTTGG CTAGACGCCA GCCCCAGATT 
GAGCCCATTA ACGATGCCTC CTCGTCGGCA GTCTGGTCAT CTATTGCCGC CAGCAAATCA 
CAAAAGGGTA GCCACAAGGA CCACCGCGAG ATGGTTCAAT ACGATGAAGA CTTCTTGCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648241.1
CDS	137..979
Translation	MVYIHFPNNLTEEEQMLQAKYQKLKKKKKALQAHKAPKPEPESSLTLKRPTDARDAREVARKLIKSGAIPAIQKQTKQDQTSFKRPKGQERAKRSTSETTVASYQPFSSTQNDVAQETIISEIIKEEPRRQNLYQHFATERDREERGMPEKVPMDTAQPEKPRAGNTIFVSGNKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEIHGKNVNGINLQVQLARRQPQIEPINDASSSAVWSSIAASKSQKGSHKDHREMVQYDEDFLL

Target ORF information:

RefSeq Version	NM_139984.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster negative elongation factor E (Nelf-E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139984.3

ORF Insert Sequence:

ATGGTTTACA TACACTTCCC CAACAATTTA ACCGAAGAGG AGCAAATGCT GCAGGCCAAG 
TATCAGAAAC TCAAGAAAAA GAAAAAGGCA CTGCAAGCGC ACAAGGCGCC CAAGCCGGAG 
CCGGAGAGCT CCTTGACCTT GAAGCGTCCC ACGGATGCGC GAGATGCCCG TGAAGTGGCC 
AGGAAACTAA TCAAAAGCGG CGCCATTCCC GCCATCCAGA AGCAGACCAA GCAAGATCAA 
ACCTCCTTCA AGCGGCCCAA AGGACAGGAA AGAGCCAAGC GCTCCACCTC GGAAACCACT 
GTTGCCTCCT ACCAGCCCTT TTCGTCTACG CAGAACGACG TGGCCCAGGA GACGATCATT 
AGTGAGATCA TTAAGGAGGA GCCGCGTCGA CAGAATCTCT ACCAACACTT CGCCACCGAG 
CGGGATCGAG AGGAGCGCGG CATGCCCGAG AAGGTGCCCA TGGACACAGC GCAGCCAGAG 
AAGCCACGGG CCGGCAATAC CATCTTCGTG TCCGGCAACA AGGTCACCGA GGATTTCTTA 
AAAAAGACAT TCAACGACTA CGGCACCATT GTCAATGTCT CCATGGAGAT CGAAAAGAGT 
CGCGGCTTTG TTTCCTTCGC CAAACCCGAG TCGGCAGATC GCGCCATTGC GGAGATTCAT 
GGCAAGAATG TGAATGGCAT CAACTTGCAG GTGCAGTTGG CTAGACGCCA GCCCCAGATT 
GAGCCCATTA ACGATGCCTC CTCGTCGGCA GTCTGGTCAT CTATTGCCGC CAGCAAATCA 
CAAAAGGGTA GCCACAAGGA CCACCGCGAG ATGGTTCAAT ACGATGAAGA CTTCTTGCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262

Nelf-E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

FAX