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fz2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol fz2
Entrez Gene ID 40090
Full Name frizzled 2
Synonyms CG9739,D-Fz2,D-fz2,DFZ2,DFz-2,DFz2,Dfrz2,Dfz2,Dfz[[2]],Dm Fz2,Dmel\CG9739,FZ2,Fz2,dFz2,dfz(2),dfz2,dfz2r,frz2,fz-2
General protein information
Preferred Names

frizzled 2

Names

CG9739 gene product from transcript CG9739-RG
CG9739-PA
CG9739-PB
CG9739-PC
CG9739-PD
CG9739-PE
CG9739-PF
CG9739-PG
D-frizzled2
DFrizzled2
Dfrizzled[[2]]
Frizzled2
drosophila frizzled 2
frizzled-2
fz2-PA
fz2-PB
fz2-PC
fz2-PD
fz2-PE
fz2-PF
fz2-PG
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

3L

75F9-76A3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001104173.3 NP_001097643.1 frizzled 2, isoform C
NM_001275108.2 NP_001262037.1 frizzled 2, isoform F
NM_168787.3 NP_730389.1 frizzled 2, isoform B
NM_001144499.3 NP_001137971.1 frizzled 2, isoform D
NM_079431.2 NP_524155.1 frizzled 2, isoform A
NM_001275107.1 NP_001262036.1 frizzled 2, isoform E
NM_001275109.1 NP_001262038.1 frizzled 2, isoform G

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) FZD5 XP_526008.2
Canis lupus familiaris (dog) FZD5 XP_003640237.1
Rattus norvegicus (Norway rat) Fzd5 NP_776210.1
Danio rerio (zebrafish) fzd5 NP_571209.2
LOC710796 XP_002799085.2
Drosophila melanogaster (fruit fly) fz2 NP_001262036.1
Bos taurus (cattle) FZD5 XP_005202823.1
Gallus gallus (chicken) FZD5 XP_004942694.1
Anopheles gambiae (African malaria mosquito) GPRFZ2 XP_311505.1
Homo sapiens (human) FZD5 NP_003459.2
Mus musculus (house mouse) Fzd5 NP_001036124.1
Xenopus tropicalis (tropical clawed frog) fzd5 XP_004917802.1

Gene Ontology


Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Maturation of a central brain flight circuit in Drosophila requires Fz2 and Ca2+ signaling.
Title: Maturation of a central brain flight circuit in Drosophila requires Fz2/Ca?? signaling.

The heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse and Ankyrin 2 as a target of Go signaling required for neuromuscular junction formation
Title: Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.

Study finds that C-terminal fragments of the Wg receptor DFz2 accumulate in nuclear foci in association with large RNA granules localizing to the space between the inner & and outer nuclear membranes; granules are found at of inner membrane invaginations, are bounded by LamC, and can be seen leaving the nucleus.
Title: Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling.

Knockdown of Tc-fz1 alone interfered with the formation of the proximo-distal and the dorso-ventral axes during leg development, whereas no effect was observed with single Tc-fz2 or Tc-fz4 RNAi knockdowns.
Title: A context-dependent combination of Wnt receptors controls axis elongation and leg development in a short germ insect.

Wnt-activated growth of the postsynaptic membrane is mediated by the synapse-to-nucleus translocation and active nuclear import of Fz2-C via a selective Importin-beta11/alpha2 pathway.
Title: The nuclear import of Frizzled2-C by Importins-beta11 and alpha2 promotes postsynaptic development.

The following fz2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fz2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa18934 NM_001104173.3
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant C, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa18934 NM_079431.2
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa18934 NM_001144499.3
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant D, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa18934 NM_001275107.1
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant E, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa18934 NM_168787.3
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa18934 NM_001275109.1
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant G, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OFa21076 NM_001275108.2
Latest version!		 Drosophila melanogaster frizzled 2 (fz2), transcript variant F, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa18934
Clone ID Related Accession (Same CDS sequence) NM_001104173.3 , NM_168787.3 , NM_001144499.3 , NM_079431.2 , NM_001275107.1 , NM_001275109.1
Accession Version NM_001104173.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104173.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001097643.1
CDS1060..3144
Translation

Target ORF information:

RefSeq Version NM_001104173.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant C, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001104173.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa18934
Clone ID Related Accession (Same CDS sequence) NM_001104173.3 , NM_168787.3 , NM_001144499.3 , NM_079431.2 , NM_001275107.1 , NM_001275109.1
Accession Version NM_168787.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168787.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_730389.1
CDS451..2535
Translation

Target ORF information:

RefSeq Version NM_168787.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_168787.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa18934
Clone ID Related Accession (Same CDS sequence) NM_001104173.3 , NM_168787.3 , NM_001144499.3 , NM_079431.2 , NM_001275107.1 , NM_001275109.1
Accession Version NM_001144499.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform D
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144499.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137971.1
CDS1209..3293
Translation

Target ORF information:

RefSeq Version NM_001144499.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant D, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001144499.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa18934
Clone ID Related Accession (Same CDS sequence) NM_001104173.3 , NM_168787.3 , NM_001144499.3 , NM_079431.2 , NM_001275107.1 , NM_001275109.1
Accession Version NM_079431.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Nov 6, 2002 this sequence version replaced NM_079431.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524155.1
CDS285..2369
Translation

Target ORF information:

RefSeq Version NM_079431.2
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_079431.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa18934
Clone ID Related Accession (Same CDS sequence) NM_001104173.3 , NM_168787.3 , NM_001144499.3 , NM_079431.2 , NM_001275107.1 , NM_001275109.1
Accession Version NM_001275107.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform E
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001262036.1
CDS957..3041
Translation

Target ORF information:

RefSeq Version NM_001275107.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001275107.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa18934
Clone ID Related Accession (Same CDS sequence) NM_001104173.3 , NM_168787.3 , NM_001144499.3 , NM_079431.2 , NM_001275107.1 , NM_001275109.1
Accession Version NM_001275109.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform G
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001262038.1
CDS385..2469
Translation

Target ORF information:

RefSeq Version NM_001275109.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant G, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001275109.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa21076
Clone ID Related Accession (Same CDS sequence) NM_001275108.2
Accession Version NM_001275108.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product frizzled 2, isoform F
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001275108.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGACATGG AGAGTCGGGG 
GGAGCATCGA CCATGGGCGG CGGTGGGGGC GGCGGTAGCA CCCTTGGCGG CGGCACCCTG 
GGCCACGGCA CCGCGATGAG CAGCAGCACG GTCGGCATGG GCCCTCTCCC CGGCACCCTG 
ATGCATGGCG CCTCCGGCGG AGGCGTGGGC GGCGGCAATC CCGGCAACGT TTACGGCAAT 
GGCAACAACG GAGTGGGCGG ACAGAATACC GCTCCCGGCT CCGTGATCGA CTCGCGGGCC 
GTGTCCGTGG TGGTCACGCT GCCCGGCGGC CCGGGTGCCC AGCATCCTGG CCAGGCGCTC 
AGCGACTATG GTCCCATATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001262037.1
CDS1060..3480
Translation

Target ORF information:

RefSeq Version NM_001275108.2
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster frizzled 2 (fz2), transcript variant F, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001275108.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAGACACA ATCGACTGAA GGTCCTGATC CTGGGACTCG TCCTCCTGCT GACATCTTGT 
CGAGCGGATG GACCGCTGCA CAGTGCGGAT CACGGCATGG GCGGAATGGG CATGGGTGGT 
CACGGCCTGG ACGCGAGTCC CGCACCCGGT TACGGAGTGC CAGTCATACC CAAGGATCCC 
AATCTGCGAT GCGAGGAGAT CACCATACCA ATGTGTCGGG GCATTGGCTA CAACATGACA 
TCCTTCCCCA ACGAAATGAA CCATGAGACC CAGGACGAAG CGGGCCTGGA GGTGCACCAG 
TTCTGGCCCC TGGTGGAGAT CAAATGCTCG CCGGACCTCA AGTTCTTCCT GTGCAGCATG 
TACACGCCCA TCTGCCTGGA GGATTACCAC AAGCCGCTGC CCGTTTGCCG GAGTGTCTGC 
GAGAGAGCCC GCTCGGGATG CGCACCCATC ATGCAGCAGT ACAGCTTCGA ATGGCCGGAG 
AGAATGGCGT GCGAGCACTT GCCTCTTCAT GGTGACCCCG ACAATCTGTG CATGGAACAG 
CCCTCGTACA CGGAGGCTGG CAGCGGTGGC AGCTCGGGCG GATCGGGTGG CTCTGGCAGC 
GGTTCCGGCT CCGGCGGCAA ACGGAAGCAA GGAGGCAGTG GCTCGGGCGG CAGTGGGGCC 
GGCGGCAGCA GCGGTTCCAC CTCAACGAAG CCGTGCCGCG GACGCAATTC AAAAAACTGC 
CAAAATCCCC AAGGAGAAAA GGCAAGCGGA AAAGAGTGCA GCTGCTCGTG CCGCTCCCCA 
CTCATCTTCC TGGGGAAGGA GCAGCTGCTG CAGCAGCAGT CGCAGATGCC CATGATGCAC 
CATCCACACC ACTGGTACAT GAACCTCACT GTCCAAAGGA TCGCCGGCGT TCCAAACTGC 
GGCATACCGT GCAAGGGGCC CTTCTTCAGC AACGACGAAA AGGATTTCGC CGGCCTCTGG 
ATCGCCCTGT GGTCGGGACT GTGCTTCTGC AGCACGCTCA TGACCCTAAC CACATTCATC 
ATCGACACCG AAAGGTTTAA GTACCCGGAG CGGCCCATTG TCTTCCTCTC CGCCTGCTAC 
TTCATGGTGG CAGTGGGCTA CCTGTCGCGC AACTTCCTGC AGAACGAGGA GATCGCCTGC 
GACGGCCTGC TGCTCCGGGA AAGCTCCACG GGTCCGCACT CGTGCACCCT GGTCTTCCTG 
CTTACCTACT TCTTTGGCAT GGCCTCGTCC ATCTGGTGGG TGATCCTCAG TTTCACCTGG 
TTCCTGGCCG CTGGTCTGAA GTGGGGCAAT GAGGCCATCA CCAAGCACTC GCAGTACTTC 
CACCTGGCCG CCTGGTTGAT TCCCACTGTC CAGTCCGTGG CCGTACTCCT GCTCTCGGCG 
GTGGATGGCG ATCCCATTCT GGGCATCTGC TATGTGGGCA ACCTCAATCC GGATCACCTA 
AAGACCTTTG TGCTGGCCCC GCTCTTCGTT TACCTCGTAA TCGGCACCAC CTTCCTGATG 
GCCGGCTTTG TGTCCCTCTT CCGCATCCGC TCGGTTATCA AGCAACAGGG CGGTGTAGGA 
GCTGGTGTCA AGGCGGACAA GCTGGAGAAA CTGATGATCA GGATTGGCAT CTTCTCGGTG 
CTCTACACGG TGCCGGCCAC CATAGTTATC GGATGTTACC TGTACGAAGC AGCCTACTTT 
GAGGACTGGA TCAAGGCCCT GGCCTGTCCA TGCGCCCAGG TGAAGGGTCC CGGCAAGAAG 
CCTCTCTACT CGGTCCTGAT GCTCAAGTAC TTCATGGCCC TGGCCGTGGG CATCACCTCG 
GGCGTGTGGA TCTGGTCTGG CAAGACGCTG GAGAGCTGGC GACGCTTCTG GCGGAGACTC 
CTAGGAGCGC CGGACCGCAC GGGCGCCAAC CAGGCGCTGA TCAAGCAGCG GCCTCCGATC 
CCGCATCCCT ATGCCGGATC TGGAATGGGA ATGCCCGTGG GCTCGGCGGC GGGCTCCCTG 
CTGGCCACGC CCTACACCCA GGCGGGCGGA GCCTCGGTGG CCTCCACCAG CCACCACCAC 
CTGCACCACC ACGTTCTCAA GCAGCCGGCG GCCAGCCACG TATGACATGG AGAGTCGGGG 
GGAGCATCGA CCATGGGCGG CGGTGGGGGC GGCGGTAGCA CCCTTGGCGG CGGCACCCTG 
GGCCACGGCA CCGCGATGAG CAGCAGCACG GTCGGCATGG GCCCTCTCCC CGGCACCCTG 
ATGCATGGCG CCTCCGGCGG AGGCGTGGGC GGCGGCAATC CCGGCAACGT TTACGGCAAT 
GGCAACAACG GAGTGGGCGG ACAGAATACC GCTCCCGGCT CCGTGATCGA CTCGCGGGCC 
GTGTCCGTGG TGGTCACGCT GCCCGGCGGC CCGGGTGCCC AGCATCCTGG CCAGGCGCTC 
AGCGACTATG GTCCCATATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,


book

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,


book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D