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svp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol svp
Entrez Gene ID 41491
Full Name seven up
Synonyms 0005/12,0042/01,0106/18,0114/20,0351/01,0598/01,0861/07,BcDNA:GH08189,CG11502,CG18158,Dmel\CG11502,NR2F3,SVP,SVP-1,SVP1,Svp,ck16,don,l(3)07842,l(3)87Bd,l(3)ck16,l(3)j2E2,l(3)rA028,l(3)rL069
General protein information
Preferred Names

seven up

Names

CG11502 gene product from transcript CG11502-RC
CG11502-PA
CG11502-PB
CG11502-PC
CG11502-PE
SEVEN-UP
complementation group 7
down-and-out
seven Up
sevenup
svp-PA
svp-PB
svp-PC
svp-PE
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

3R

87B4-87B5

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_169459.2 NP_731682.1 seven up, isoform C
NM_079601.3 NP_524325.1 seven up, isoform B
NM_001275570.1 NP_001262499.1 seven up, isoform E
NM_169458.1 NP_731681.1 seven up, isoform A

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Nr2f2 NP_033827.2
Drosophila melanogaster (fruit fly) svp NP_524325.1
Homo sapiens (human) NR2F2 NP_066285.1
NR2F2 XP_001099957.1
Pan troglodytes (chimpanzee) NR2F2 XP_003952785.1
Canis lupus familiaris (dog) NR2F2 XP_005618373.1
Bos taurus (cattle) NR2F2 NP_776827.1
Rattus norvegicus (Norway rat) Nr2f2 NP_542956.1
Gallus gallus (chicken) NR2F2 NP_989752.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002544 XP_312394.4
Xenopus tropicalis (tropical clawed frog) nr2f2 NP_001107703.1
Danio rerio (zebrafish) nr2f2 NP_571258.1

Gene Ontology


Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel allele of svp was isolated. Expression analysis showed that svp is expressed in two distinct pulses in the embryonic NB5-6T lineage.
Title: Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development.

Dan/Danr and Svp act in parallel pathways to limit Hb expression and allow neuroblasts to transition from making early-born neurons to late-born neurons at the proper time.
Title: The pipsqueak-domain proteins Distal antenna and Distal antenna-related restrict Hunchback neuroblast expression and early-born neuronal identity.

Normal patterning of alary muscles along the dorsal vessel was found to be a function of the Bithorax Complex genes abdominal-A (abd-A) and Ultrabithorax (Ubx) but not of the orphan nuclear receptor gene svp.
Title: Bithorax complex genes control alary muscle patterning along the cardiac tube of Drosophila.

N represses Svp expression, thereby exposing an underlying stochastic choice between the R7 and R8 photoreceptor fates, and then tips this choice towards the R7 fate.
Title: Loss of seven-up from Drosophila R1/R6 photoreceptors reveals a stochastic fate choice that is normally biased by Notch.

Enhancer of svp is initially activated by the NK homeodomain factor Tinman (Tin) via two conserved Tin binding sites.
Title: The NK homeodomain transcription factor Tinman is a direct activator of seven-up in the Drosophila dorsal vessel.

The following svp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the svp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04658 NM_169459.2
Latest version!		 Drosophila melanogaster seven up (svp), transcript variant C, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OFa04659 NM_169458.1
Latest version!		 Drosophila melanogaster seven up (svp), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $426.30
$609.00
OFa04660 NM_079601.3
Latest version!		 Drosophila melanogaster seven up (svp), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00
OFa08337 NM_001275570.1
Latest version!		 Drosophila melanogaster seven up (svp), transcript variant E, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa04658
Clone ID Related Accession (Same CDS sequence) NM_169459.2
Accession Version NM_169459.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product seven up, isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169459.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGCATGA GACGCGAAGC TGTTCAACGT GGACGCGTAC CACCCACTCA GCCCGGTCTG 
GCCGGCATGC ATGGGCAGTA CCAGATTGCC AACGGGGATC CCATGGGCAT TGCCGGCTTT 
AACGGGCACT CGTACCTCAG TTCCTACATC TCGCTCCTGC TGCGGGCGGA ACCGTATCCG 
ACTTCGCGAT ATGGCCAGTG CATGCAACCC AACAACATTA TGGGCATCGA CAACATCTGC 
GAACTGGCCG CCCGACTGCT CTTCTCGGCG GTCGAGTGGG CCAAGAACAT ACCCTTCTTC 
CCGGAGCTGC AGGTGACCGA CCAGGTGGCC CTGCTCCGGC TCGTCTGGTC AGAGCTCTTC 
GTCCTAAACG CCAGCCAGTG CTCCATGCCG CTCCATGTGG CGCCACTGCT GGCCGCCGCC 
GGACTTCATG CCTCCCCGAT GGCCGCCGAT CGTGTGGTGG CCTTCATGGA CCACATCCGC 
ATCTTCCAGG AGCAGGTGGA GAAGCTGAAG GCGCTGCATG TCGACTCCGC GGAGTACTCC 
TGCCTCAAGG CGATCGTGCT CTTCACCACC GATGCCTGCG GCCTGTCCGA TGTGACGCAC 
ATTGAATCCC TGCAAGAGAA GTCGCAGTGC GCCCTCGAGG AATACTGCCG GACCCAGTAT 
CCCAACCAGC CCACGAGATT CGGCAAGCTG CTTCTCAGAC TGCCATCGCT GCGAACGGTC 
TCCTCACAAG TCATTGAGCA ATTGTTTTTT GTGCGTCTAG TCGGAAAAAC GCCAATTGAA 
ACGCTGATAC GCGATATGCT GCTGAGCGGC AACAGTTTCT CCTGGCCCTA TCTGCCTTCG 
ATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_731682.1
CDS247..1092
Translation

Target ORF information:

RefSeq Version NM_169459.2
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster seven up (svp), transcript variant C, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_169459.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGCATGA GACGCGAAGC TGTTCAACGT GGACGCGTAC CACCCACTCA GCCCGGTCTG 
GCCGGCATGC ATGGGCAGTA CCAGATTGCC AACGGGGATC CCATGGGCAT TGCCGGCTTT 
AACGGGCACT CGTACCTCAG TTCCTACATC TCGCTCCTGC TGCGGGCGGA ACCGTATCCG 
ACTTCGCGAT ATGGCCAGTG CATGCAACCC AACAACATTA TGGGCATCGA CAACATCTGC 
GAACTGGCCG CCCGACTGCT CTTCTCGGCG GTCGAGTGGG CCAAGAACAT ACCCTTCTTC 
CCGGAGCTGC AGGTGACCGA CCAGGTGGCC CTGCTCCGGC TCGTCTGGTC AGAGCTCTTC 
GTCCTAAACG CCAGCCAGTG CTCCATGCCG CTCCATGTGG CGCCACTGCT GGCCGCCGCC 
GGACTTCATG CCTCCCCGAT GGCCGCCGAT CGTGTGGTGG CCTTCATGGA CCACATCCGC 
ATCTTCCAGG AGCAGGTGGA GAAGCTGAAG GCGCTGCATG TCGACTCCGC GGAGTACTCC 
TGCCTCAAGG CGATCGTGCT CTTCACCACC GATGCCTGCG GCCTGTCCGA TGTGACGCAC 
ATTGAATCCC TGCAAGAGAA GTCGCAGTGC GCCCTCGAGG AATACTGCCG GACCCAGTAT 
CCCAACCAGC CCACGAGATT CGGCAAGCTG CTTCTCAGAC TGCCATCGCT GCGAACGGTC 
TCCTCACAAG TCATTGAGCA ATTGTTTTTT GTGCGTCTAG TCGGAAAAAC GCCAATTGAA 
ACGCTGATAC GCGATATGCT GCTGAGCGGC AACAGTTTCT CCTGGCCCTA TCTGCCTTCG 
ATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa04659
Clone ID Related Accession (Same CDS sequence) NM_169458.1
Accession Version NM_169458.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product seven up, isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTGCGCCT CCCCGTCCAC GGCTCCCGGC TTCTTCAATC CGCGCCCGCA ATCCGGCGCG 
GAGCTGTCGG CCTTCGACAT CGGCCTGTCC CGCTCGATGG GGCTGGGCGT CCCGCCACAC 
AGCGCCTGGC ACGAACCGCC GGCCTCCCTC GGCGGTCACC TGCACGCCGC CTCCGCCGGA 
CCGGGCACCA CCACCGGCAG CGTGGCGACG GGCGGCGGCG GTACCACTCC GAGCAGCGTG 
GCCAGCCAGC AGTCGGCGGT GATCAAGCAG GATCTCTCTT GCCCCAGCCT CAATCAGGCG 
GGATCCGGCC ATCATCCTGG CATCAAGGAG GATCTGTCCA GCAGCCTGCC CAGCGCCAAT 
GGTGGATCCG CCGGTGGCCA TCACTCCGGT TCCGGATCGG GTTCCGGTTC GGGCGTCAAT 
CCCGGACACG GCTCCGACAT GCTGCCCCTG ATCAAGGGCC ATGGCCAGGA CATGCTCACC 
AGCATCAAGG GACAGCCCAC GGGCTGTGGC AGCACCACGC CCAGCTCCCA GGCGAATAGC 
TCCCACTCGC AGAGCAGCAA CAGTGGATCC CAGATCGACA GCAAGCAGAA CATCGAGTGC 
GTCGTCTGCG GCGACAAGAG TTCCGGCAAG CACTACGGAC AATTTACCTG CGAAGGTTGC 
AAATCTTTCT TCAAGCGCTC GGTGCGACGT AATCTAACTT ACTCTTGCCG CGGCAGCAGA 
AACTGTCCCA TAGATCAACA CCATCGCAAT CAATGTCAAT ATTGTCGATT GAAGAAGTGC 
CTCAAAATGG GCATGAGACG CGAAGCTGTT CAACGTGGAC GCGTACCACC CACTCAGCCC 
GGTCTGGCCG GCATGCATGG GCAGTACCAG ATTGCCAACG GGGATCCCAT GGGCATTGCC 
GGCTTTAACG GGCACTCGTA CCTCAGTTCC TACATCTCGC TCCTGCTGCG GGCGGAACCG 
TATCCGACTT CGCGATATGG CCAGTGCATG CAACCCAACA ACATTATGGG CATCGACAAC 
ATCTGCGAAC TGGCCGCCCG ACTGCTCTTC TCGGCGGTCG AGTGGGCCAA GAACATACCC 
TTCTTCCCGG AGCTGCAGGT GACCGACCAG GTGGCCCTGC TCCGGCTCGT CTGGTCAGAG 
CTCTTCGTCC TAAACGCCAG CCAGTGCTCC ATGCCGCTCC ATGTGGCGCC ACTGCTGGCC 
GCCGCCGGAC TTCATGCCTC CCCGATGGCC GCCGATCGTG TGGTGGCCTT CATGGACCAC 
ATCCGCATCT TCCAGGAGCA GGTGGAGAAG CTGAAGGCGC TGCATGTCGA CTCCGCGGAG 
TACTCCTGCC TCAAGGCGAT CGTGCTCTTC ACCACCGGTA AGTTGCTGGA CATTCTGTAC 
AAGGATGTGC CGGCACTGCT CACCAAGGTT TCAGCACTGC TTGGCAAGGG TTCCACCGCA 
TCCAATGACG ATGTCCTGGC TGTGGTCCGT GACCACCTAG ACGAACTGAA TCGTCAGGAA 
CAGGAGTCTC AGGCTCAGCA GCAGGCGCCG CTTCACCTGG CAGCCTTCAT GAATTGCGTG 
GCCGGCGTTG AGGCTGCAGT GCAGCAGGCG GAACAGGCTC AGGTACCCAC GTCCTCTGCC 
TCCGCTTCCG TCTCTGCTCC CCTTGTTCCC TCCGCCGGCA GTGCGTTCAG CAGCTGCCAG 
GCCAAGTCCG CCGGCTCCGA GATGGACCTG CTGGCCAGTT TGTATGCTCA GGCCCAGGCC 
ACGCCCCCAA GCAGCGGCGG AGGCGACGCC TCCGGTCACA ACAACAGCAG CGGGCTAGGC 
GCCTCGCTTC CTACTCAATC GCAAAGCGGT TCGTCCTCCC GCAACCTAAC CGCCTCGCCG 
CTGAGTACTT CGCTGGCAAC CGCTCCCGCT CCAGCTTCCG CTTCAGCTCC CGCTCCCGTG 
CCCACCTCCT CCGTGGCCCA AGTCCCTGTG CCCGCACCCG TCCCAGTAAC CTCCTCCGCC 
TCCTCCTCCT CGCTGGGTGG TGGAGCCTAT CAGACGCCCT CGGCTGCGGC GGCGGCGGCG 
GCAATGTTCC ACTATCAGAC GCCACCGCGC GCCGCCTTCG GTTCGGCCTT CGATATGTTC 
CACCACAGCA CGCCCTTCGG AGTGGGGGTG GGTCACGCCC ACGCCCTTGC CCACTCGAGC 
GGCAGCGGCA GCGCGTCCTT TGGCAGTCCT AGTTACAGGT ATTCGCCCTA TAGCCTGGCC 
GGTAGTAGAT GGCAGTTGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_731681.1
CDS486..2726
Translation

Target ORF information:

RefSeq Version NM_169458.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster seven up (svp), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_169458.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTGCGCCT CCCCGTCCAC GGCTCCCGGC TTCTTCAATC CGCGCCCGCA ATCCGGCGCG 
GAGCTGTCGG CCTTCGACAT CGGCCTGTCC CGCTCGATGG GGCTGGGCGT CCCGCCACAC 
AGCGCCTGGC ACGAACCGCC GGCCTCCCTC GGCGGTCACC TGCACGCCGC CTCCGCCGGA 
CCGGGCACCA CCACCGGCAG CGTGGCGACG GGCGGCGGCG GTACCACTCC GAGCAGCGTG 
GCCAGCCAGC AGTCGGCGGT GATCAAGCAG GATCTCTCTT GCCCCAGCCT CAATCAGGCG 
GGATCCGGCC ATCATCCTGG CATCAAGGAG GATCTGTCCA GCAGCCTGCC CAGCGCCAAT 
GGTGGATCCG CCGGTGGCCA TCACTCCGGT TCCGGATCGG GTTCCGGTTC GGGCGTCAAT 
CCCGGACACG GCTCCGACAT GCTGCCCCTG ATCAAGGGCC ATGGCCAGGA CATGCTCACC 
AGCATCAAGG GACAGCCCAC GGGCTGTGGC AGCACCACGC CCAGCTCCCA GGCGAATAGC 
TCCCACTCGC AGAGCAGCAA CAGTGGATCC CAGATCGACA GCAAGCAGAA CATCGAGTGC 
GTCGTCTGCG GCGACAAGAG TTCCGGCAAG CACTACGGAC AATTTACCTG CGAAGGTTGC 
AAATCTTTCT TCAAGCGCTC GGTGCGACGT AATCTAACTT ACTCTTGCCG CGGCAGCAGA 
AACTGTCCCA TAGATCAACA CCATCGCAAT CAATGTCAAT ATTGTCGATT GAAGAAGTGC 
CTCAAAATGG GCATGAGACG CGAAGCTGTT CAACGTGGAC GCGTACCACC CACTCAGCCC 
GGTCTGGCCG GCATGCATGG GCAGTACCAG ATTGCCAACG GGGATCCCAT GGGCATTGCC 
GGCTTTAACG GGCACTCGTA CCTCAGTTCC TACATCTCGC TCCTGCTGCG GGCGGAACCG 
TATCCGACTT CGCGATATGG CCAGTGCATG CAACCCAACA ACATTATGGG CATCGACAAC 
ATCTGCGAAC TGGCCGCCCG ACTGCTCTTC TCGGCGGTCG AGTGGGCCAA GAACATACCC 
TTCTTCCCGG AGCTGCAGGT GACCGACCAG GTGGCCCTGC TCCGGCTCGT CTGGTCAGAG 
CTCTTCGTCC TAAACGCCAG CCAGTGCTCC ATGCCGCTCC ATGTGGCGCC ACTGCTGGCC 
GCCGCCGGAC TTCATGCCTC CCCGATGGCC GCCGATCGTG TGGTGGCCTT CATGGACCAC 
ATCCGCATCT TCCAGGAGCA GGTGGAGAAG CTGAAGGCGC TGCATGTCGA CTCCGCGGAG 
TACTCCTGCC TCAAGGCGAT CGTGCTCTTC ACCACCGGTA AGTTGCTGGA CATTCTGTAC 
AAGGATGTGC CGGCACTGCT CACCAAGGTT TCAGCACTGC TTGGCAAGGG TTCCACCGCA 
TCCAATGACG ATGTCCTGGC TGTGGTCCGT GACCACCTAG ACGAACTGAA TCGTCAGGAA 
CAGGAGTCTC AGGCTCAGCA GCAGGCGCCG CTTCACCTGG CAGCCTTCAT GAATTGCGTG 
GCCGGCGTTG AGGCTGCAGT GCAGCAGGCG GAACAGGCTC AGGTACCCAC GTCCTCTGCC 
TCCGCTTCCG TCTCTGCTCC CCTTGTTCCC TCCGCCGGCA GTGCGTTCAG CAGCTGCCAG 
GCCAAGTCCG CCGGCTCCGA GATGGACCTG CTGGCCAGTT TGTATGCTCA GGCCCAGGCC 
ACGCCCCCAA GCAGCGGCGG AGGCGACGCC TCCGGTCACA ACAACAGCAG CGGGCTAGGC 
GCCTCGCTTC CTACTCAATC GCAAAGCGGT TCGTCCTCCC GCAACCTAAC CGCCTCGCCG 
CTGAGTACTT CGCTGGCAAC CGCTCCCGCT CCAGCTTCCG CTTCAGCTCC CGCTCCCGTG 
CCCACCTCCT CCGTGGCCCA AGTCCCTGTG CCCGCACCCG TCCCAGTAAC CTCCTCCGCC 
TCCTCCTCCT CGCTGGGTGG TGGAGCCTAT CAGACGCCCT CGGCTGCGGC GGCGGCGGCG 
GCAATGTTCC ACTATCAGAC GCCACCGCGC GCCGCCTTCG GTTCGGCCTT CGATATGTTC 
CACCACAGCA CGCCCTTCGG AGTGGGGGTG GGTCACGCCC ACGCCCTTGC CCACTCGAGC 
GGCAGCGGCA GCGCGTCCTT TGGCAGTCCT AGTTACAGGT ATTCGCCCTA TAGCCTGGCC 
GGTAGTAGAT GGCAGTTGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa04660
Clone ID Related Accession (Same CDS sequence) NM_079601.3
Accession Version NM_079601.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product seven up, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079601.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTGCGCCT CCCCGTCCAC GGCTCCCGGC TTCTTCAATC CGCGCCCGCA ATCCGGCGCG 
GAGCTGTCGG CCTTCGACAT CGGCCTGTCC CGCTCGATGG GGCTGGGCGT CCCGCCACAC 
AGCGCCTGGC ACGAACCGCC GGCCTCCCTC GGCGGTCACC TGCACGCCGC CTCCGCCGGA 
CCGGGCACCA CCACCGGCAG CGTGGCGACG GGCGGCGGCG GTACCACTCC GAGCAGCGTG 
GCCAGCCAGC AGTCGGCGGT GATCAAGCAG GATCTCTCTT GCCCCAGCCT CAATCAGGCG 
GGATCCGGCC ATCATCCTGG CATCAAGGAG GATCTGTCCA GCAGCCTGCC CAGCGCCAAT 
GGTGGATCCG CCGGTGGCCA TCACTCCGGT TCCGGATCGG GTTCCGGTTC GGGCGTCAAT 
CCCGGACACG GCTCCGACAT GCTGCCCCTG ATCAAGGGCC ATGGCCAGGA CATGCTCACC 
AGCATCAAGG GACAGCCCAC GGGCTGTGGC AGCACCACGC CCAGCTCCCA GGCGAATAGC 
TCCCACTCGC AGAGCAGCAA CAGTGGATCC CAGATCGACA GCAAGCAGAA CATCGAGTGC 
GTCGTCTGCG GCGACAAGAG TTCCGGCAAG CACTACGGAC AATTTACCTG CGAAGGTTGC 
AAATCTTTCT TCAAGCGCTC GGTGCGACGT AATCTAACTT ACTCTTGCCG CGGCAGCAGA 
AACTGTCCCA TAGATCAACA CCATCGCAAT CAATGTCAAT ATTGTCGATT GAAGAAGTGC 
CTCAAAATGG GCATGAGACG CGAAGCTGTT CAACGTGGAC GCGTACCACC CACTCAGCCC 
GGTCTGGCCG GCATGCATGG GCAGTACCAG ATTGCCAACG GGGATCCCAT GGGCATTGCC 
GGCTTTAACG GGCACTCGTA CCTCAGTTCC TACATCTCGC TCCTGCTGCG GGCGGAACCG 
TATCCGACTT CGCGATATGG CCAGTGCATG CAACCCAACA ACATTATGGG CATCGACAAC 
ATCTGCGAAC TGGCCGCCCG ACTGCTCTTC TCGGCGGTCG AGTGGGCCAA GAACATACCC 
TTCTTCCCGG AGCTGCAGGT GACCGACCAG GTGGCCCTGC TCCGGCTCGT CTGGTCAGAG 
CTCTTCGTCC TAAACGCCAG CCAGTGCTCC ATGCCGCTCC ATGTGGCGCC ACTGCTGGCC 
GCCGCCGGAC TTCATGCCTC CCCGATGGCC GCCGATCGTG TGGTGGCCTT CATGGACCAC 
ATCCGCATCT TCCAGGAGCA GGTGGAGAAG CTGAAGGCGC TGCATGTCGA CTCCGCGGAG 
TACTCCTGCC TCAAGGCGAT CGTGCTCTTC ACCACCGATG CCTGCGGCCT GTCCGATGTG 
ACGCACATTG AATCCCTGCA AGAGAAGTCG CAGTGCGCCC TCGAGGAATA CTGCCGGACC 
CAGTATCCCA ACCAGCCCAC GAGATTCGGC AAGCTGCTTC TCAGACTGCC ATCGCTGCGA 
ACGGTCTCCT CACAAGTCAT TGAGCAATTG TTTTTTGTGC GTCTAGTCGG AAAAACGCCA 
ATTGAAACGC TGATACGCGA TATGCTGCTG AGCGGCAACA GTTTCTCCTG GCCCTATCTG 
CCTTCGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524325.1
CDS444..2075
Translation

Target ORF information:

RefSeq Version NM_079601.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster seven up (svp), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_079601.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTGCGCCT CCCCGTCCAC GGCTCCCGGC TTCTTCAATC CGCGCCCGCA ATCCGGCGCG 
GAGCTGTCGG CCTTCGACAT CGGCCTGTCC CGCTCGATGG GGCTGGGCGT CCCGCCACAC 
AGCGCCTGGC ACGAACCGCC GGCCTCCCTC GGCGGTCACC TGCACGCCGC CTCCGCCGGA 
CCGGGCACCA CCACCGGCAG CGTGGCGACG GGCGGCGGCG GTACCACTCC GAGCAGCGTG 
GCCAGCCAGC AGTCGGCGGT GATCAAGCAG GATCTCTCTT GCCCCAGCCT CAATCAGGCG 
GGATCCGGCC ATCATCCTGG CATCAAGGAG GATCTGTCCA GCAGCCTGCC CAGCGCCAAT 
GGTGGATCCG CCGGTGGCCA TCACTCCGGT TCCGGATCGG GTTCCGGTTC GGGCGTCAAT 
CCCGGACACG GCTCCGACAT GCTGCCCCTG ATCAAGGGCC ATGGCCAGGA CATGCTCACC 
AGCATCAAGG GACAGCCCAC GGGCTGTGGC AGCACCACGC CCAGCTCCCA GGCGAATAGC 
TCCCACTCGC AGAGCAGCAA CAGTGGATCC CAGATCGACA GCAAGCAGAA CATCGAGTGC 
GTCGTCTGCG GCGACAAGAG TTCCGGCAAG CACTACGGAC AATTTACCTG CGAAGGTTGC 
AAATCTTTCT TCAAGCGCTC GGTGCGACGT AATCTAACTT ACTCTTGCCG CGGCAGCAGA 
AACTGTCCCA TAGATCAACA CCATCGCAAT CAATGTCAAT ATTGTCGATT GAAGAAGTGC 
CTCAAAATGG GCATGAGACG CGAAGCTGTT CAACGTGGAC GCGTACCACC CACTCAGCCC 
GGTCTGGCCG GCATGCATGG GCAGTACCAG ATTGCCAACG GGGATCCCAT GGGCATTGCC 
GGCTTTAACG GGCACTCGTA CCTCAGTTCC TACATCTCGC TCCTGCTGCG GGCGGAACCG 
TATCCGACTT CGCGATATGG CCAGTGCATG CAACCCAACA ACATTATGGG CATCGACAAC 
ATCTGCGAAC TGGCCGCCCG ACTGCTCTTC TCGGCGGTCG AGTGGGCCAA GAACATACCC 
TTCTTCCCGG AGCTGCAGGT GACCGACCAG GTGGCCCTGC TCCGGCTCGT CTGGTCAGAG 
CTCTTCGTCC TAAACGCCAG CCAGTGCTCC ATGCCGCTCC ATGTGGCGCC ACTGCTGGCC 
GCCGCCGGAC TTCATGCCTC CCCGATGGCC GCCGATCGTG TGGTGGCCTT CATGGACCAC 
ATCCGCATCT TCCAGGAGCA GGTGGAGAAG CTGAAGGCGC TGCATGTCGA CTCCGCGGAG 
TACTCCTGCC TCAAGGCGAT CGTGCTCTTC ACCACCGATG CCTGCGGCCT GTCCGATGTG 
ACGCACATTG AATCCCTGCA AGAGAAGTCG CAGTGCGCCC TCGAGGAATA CTGCCGGACC 
CAGTATCCCA ACCAGCCCAC GAGATTCGGC AAGCTGCTTC TCAGACTGCC ATCGCTGCGA 
ACGGTCTCCT CACAAGTCAT TGAGCAATTG TTTTTTGTGC GTCTAGTCGG AAAAACGCCA 
ATTGAAACGC TGATACGCGA TATGCTGCTG AGCGGCAACA GTTTCTCCTG GCCCTATCTG 
CCTTCGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa08337
Clone ID Related Accession (Same CDS sequence) NM_001275570.1
Accession Version NM_001275570.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product seven up, isoform E
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTGCGCCT CCCCGTCCAC GGCTCCCGGC TTCTTCAATC CGCGCCCGCA ATCCGGCGCG 
GAGCTGTCGG CCTTCGACAT CGGCCTGTCC CGCTCGATGG GGCTGGGCGT CCCGCCACAC 
AGCGCCTGGC ACGAACCGCC GGCCTCCCTC GGCGGTCACC TGCACGCCGC CTCCGCCGGA 
CCGGGCACCA CCACCGGCAG CGTGGCGACG GGCGGCGGCG GTACCACTCC GAGCAGCGTG 
GCCAGCCAGC AGTCGGCGGT GATCAAGCAG GATCTCTCTT GCCCCAGCCT CAATCAGGCG 
GGATCCGGCC ATCATCCTGG CATCAAGGAG GATCTGTCCA GCAGCCTGCC CAGCGCCAAT 
GGTGGATCCG CCGGTGGCCA TCACTCCGGT TCCGGATCGG GTTCCGGTTC GGGCGTCAAT 
CCCGGACACG GCTCCGACAT GCTGCCCCTG ATCAAGGGCC ATGGCCAGGA CATGCTCACC 
AGCATCAAGG GACAGCCCAC GGGCTGTGGC AGCACCACGC CCAGCTCCCA GGCGAATAGC 
TCCCACTCGC AGAGCAGCAA CAGTGGATCC CAGATCGACA GCAAGCAGAA CATCGAGTGC 
GTCGTCTGCG GCGACAAGAG TTCCGGCAAG CACTACGGAC AATTTACCTG CGAAGGTTGC 
AAATCTTTCT TCAAGCGCTC GGTGCGACGT AATCTAACTT ACTCTTGCCG CGGCAGCAGA 
AACTGTCCCA TAGATCAACA CCATCGCAAT CAATGTCAAT ATTGTCGATT GAAGAAGTGC 
CTCAAAATGG GCATGAGACG CGAAGCTGTT CAACGTGGAC GCGTACCACC CACTCAGCCC 
GGTCTGGCCG GCATGCATGG GCAGTACCAG ATTGCCAACG GGGATCCCAT GGGCATTGCC 
GGCTTTAACG GGCACTCGTA CCTCAGTTCC TACATCTCGC TCCTGCTGCG GGCGGAACCG 
TATCCGACTT CGCGATATGG CCAGTGCATG CAACCCAACA ACATTATGGG CATCGACAAC 
ATCTGCGAAC TGGCCGCCCG ACTGCTCTTC TCGGCGGTCG AGTGGGCCAA GAACATACCC 
TTCTTCCCGG AGCTGCAGGT GACCGACCAG GTGGCCCTGC TCCGGCTCGT CTGGTCAGAG 
CTCTTCGTCC TAAACGCCAG CCAGTGCTCC ATGCCGCTCC ATGTGGCGCC ACTGCTGGCC 
GCCGCCGGAC TTCATGCCTC CCCGATGGCC GCCGATCGTG TGGTGGCCTT CATGGACCAC 
ATCCGCATCT TCCAGGAGCA GGTGGAGAAG CTGAAGGCGC TGCATGTCGA CTCCGCGGAG 
TACTCCTGCC TCAAGGCGAT CGTGCTCTTC ACCACCGATG CCTGCGGCCT GTCCGATGTG 
ACGCACATTG AATCCCTGCA AGAGAAGTCG CAGTGCGCCC TCGAGGAATA CTGCCGGACC 
CAGTATCCCA ACCAGCCCAC GAGATTCGGC AAGCTGCTTC TCAGACTGCC ATCGCTGCGA 
ACGGTCTCCT CACAAGTCAT TGAGCAATTG TTTTTTGTGC GTCTAGTCGG AAAAACGCCA 
ATTGAAACGC TGATACGCGA TATGCTGCTG AGCGGCAACA GTTTCTCCTG GCCCTATCTG 
CCTTCGATGT GACACACGAT GTGGCGCCAA TTGACAACAA CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001262499.1
CDS444..2108
Translation

Target ORF information:

RefSeq Version NM_001275570.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster seven up (svp), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001275570.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTGCGCCT CCCCGTCCAC GGCTCCCGGC TTCTTCAATC CGCGCCCGCA ATCCGGCGCG 
GAGCTGTCGG CCTTCGACAT CGGCCTGTCC CGCTCGATGG GGCTGGGCGT CCCGCCACAC 
AGCGCCTGGC ACGAACCGCC GGCCTCCCTC GGCGGTCACC TGCACGCCGC CTCCGCCGGA 
CCGGGCACCA CCACCGGCAG CGTGGCGACG GGCGGCGGCG GTACCACTCC GAGCAGCGTG 
GCCAGCCAGC AGTCGGCGGT GATCAAGCAG GATCTCTCTT GCCCCAGCCT CAATCAGGCG 
GGATCCGGCC ATCATCCTGG CATCAAGGAG GATCTGTCCA GCAGCCTGCC CAGCGCCAAT 
GGTGGATCCG CCGGTGGCCA TCACTCCGGT TCCGGATCGG GTTCCGGTTC GGGCGTCAAT 
CCCGGACACG GCTCCGACAT GCTGCCCCTG ATCAAGGGCC ATGGCCAGGA CATGCTCACC 
AGCATCAAGG GACAGCCCAC GGGCTGTGGC AGCACCACGC CCAGCTCCCA GGCGAATAGC 
TCCCACTCGC AGAGCAGCAA CAGTGGATCC CAGATCGACA GCAAGCAGAA CATCGAGTGC 
GTCGTCTGCG GCGACAAGAG TTCCGGCAAG CACTACGGAC AATTTACCTG CGAAGGTTGC 
AAATCTTTCT TCAAGCGCTC GGTGCGACGT AATCTAACTT ACTCTTGCCG CGGCAGCAGA 
AACTGTCCCA TAGATCAACA CCATCGCAAT CAATGTCAAT ATTGTCGATT GAAGAAGTGC 
CTCAAAATGG GCATGAGACG CGAAGCTGTT CAACGTGGAC GCGTACCACC CACTCAGCCC 
GGTCTGGCCG GCATGCATGG GCAGTACCAG ATTGCCAACG GGGATCCCAT GGGCATTGCC 
GGCTTTAACG GGCACTCGTA CCTCAGTTCC TACATCTCGC TCCTGCTGCG GGCGGAACCG 
TATCCGACTT CGCGATATGG CCAGTGCATG CAACCCAACA ACATTATGGG CATCGACAAC 
ATCTGCGAAC TGGCCGCCCG ACTGCTCTTC TCGGCGGTCG AGTGGGCCAA GAACATACCC 
TTCTTCCCGG AGCTGCAGGT GACCGACCAG GTGGCCCTGC TCCGGCTCGT CTGGTCAGAG 
CTCTTCGTCC TAAACGCCAG CCAGTGCTCC ATGCCGCTCC ATGTGGCGCC ACTGCTGGCC 
GCCGCCGGAC TTCATGCCTC CCCGATGGCC GCCGATCGTG TGGTGGCCTT CATGGACCAC 
ATCCGCATCT TCCAGGAGCA GGTGGAGAAG CTGAAGGCGC TGCATGTCGA CTCCGCGGAG 
TACTCCTGCC TCAAGGCGAT CGTGCTCTTC ACCACCGATG CCTGCGGCCT GTCCGATGTG 
ACGCACATTG AATCCCTGCA AGAGAAGTCG CAGTGCGCCC TCGAGGAATA CTGCCGGACC 
CAGTATCCCA ACCAGCCCAC GAGATTCGGC AAGCTGCTTC TCAGACTGCC ATCGCTGCGA 
ACGGTCTCCT CACAAGTCAT TGAGCAATTG TTTTTTGTGC GTCTAGTCGG AAAAACGCCA 
ATTGAAACGC TGATACGCGA TATGCTGCTG AGCGGCAACA GTTTCTCCTG GCCCTATCTG 
CCTTCGATGT GACACACGAT GTGGCGCCAA TTGACAACAA CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,


book

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,


book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D