ORF » Species Summary » Drosophila melanogaster » MED25 cDNA ORF clone

MED25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following MED25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa08556 NM_142597.4
Latest version!		 Drosophila melanogaster mediator complex subunit 25 (MED25), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MED25 ( NM_142597.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650854.1 Drosophila melanogaster mediator complex subunit 25 (MED25), mRNA.
    Price & Availability↑
    CloneID OFa08556
    Clone ID Related Accession (Same CDS sequence) NM_142597.4
    Accession Version NM_142597.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mediator complex subunit 25
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142597.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGGTGG ACCAGATTCC CCTGGCCGAC GTGGTCTTCG TCATCGAGGG CAGCGCCATC 
    AACGGAGCCT ACATCAACGA ACTAAAGACC AACTACATCC TGCCGACTTT GGAGCACTTC 
    ACCACTGGCT CCATCGACGA GCGGGAATAC CTGATTGCGG AGCGCTTTGC CACTCTGTAC 
    GGTATTGTGG TCTACCGTAC AGCGGCCAAT CTGCTGGAGC CAGTTTGTTC AACCTATGGT 
    CCCTTTCTGC AGCCCCAAAA AGTGATGGAG ACGATCGAAA GATTGCCACT AGTGGGAGGC 
    GGAATGGAGT CCTGTGCCCA CATGGCCGAA GGATTTGCTG CGGCCCACGG CTGTTTTGAT 
    GACATCAGCG AAAGGCGCCA GTTGCTCGAT CAGACCAGCG TCCAGAGGCA CTGCATTCTC 
    ATCTGCAACA GTCCGCCGTA CCAGATGCCC ACAACGGAGT CGTGGAAGTA TCCGGGCAAA 
    TCATGTGAGC AGCTGGCGGC GCTCTTCAAC GAGCGCAAAA TCAATCTCTC CATTATTGCG 
    CCACGCAAGA TGCCGGTGTT GTTCAAACTT TTCATGAAGG CCGACGGAGA TCAGCCCATT 
    ACGAGCAAAA ACTATGCCAA GAACATAAGA CACCTAGTGC TTCTAAAGGG ATACAGCCTC 
    AAGGAGAGAG CACCTAGTCC CAACAGCATG GCGGCCCAAA TGGCTGCACC CAATGCGGCA 
    CAGGCTACAG TGCAGCAGCA ACAACAACAG CAACAAAATC AAGCTGGTCA GCAACAACAA 
    GGGCAGGGCA TGCCCATGGA CACCACTCCA GCCCAGCAGC AGCAACAGCA ACAACAACAG 
    CAGCAGCAAC AGCAACAACA AGGCAATCCT CAGCAGCAGG TCATGAACAT GAACACGATG 
    CAGCAACAGC AGCCCGGTCC AAATCCCCCA GCGGGCTTAC TCAATCCACA GCAGCAGCAG 
    CAACTATTGC AGCAGCAGCA GCAGAATCAA TTCGTTTCCA ATCAAATGCA GAATCAGAAC 
    TTCCAGCAAA ACGTGGGACC CGGCCAAAAC CGCTGGATGT ATCCCAACCA GCCTGGTCAG 
    GCGCGTCCGC CCTTCATGCA GGGAGCAGGC AATGTGGGCG GTGTGGGCCA AGGCGGCGGC 
    ATGCAACAGA ATCCAAATTC TGCTCTGATA TCGCGCATTA ATGCGCCGCC GCCGAACCAA 
    ACTGTGACCT CGCTGCAGCA GCAGCAACAG CAGCAGGCGC AGCAGCAGCA GCAGCAGCAA 
    CAACAGGCCC AACAGCAGCA GCAACAAAGG ATGCAGATGC TGAGCCAGCA GCAGATGCTA 
    AATCACCAGC AGTTGCAGCA GCAACAGCAA CTGGCCCAGC AACAGCAACA GCAGCAGCAA 
    GGACAGCAAC AGCAGCAGGT AAATCCCAAT GCAGGAAACA ATATGATGCC GGCTAGCAAT 
    GCCGGCAACA TGTCTAATCC GCAGCAGCAG CAACAACAAG TAGGACAACA GTCGAATCCG 
    CAGCAGCAGG GCAATCCGCA GCAGCAACAG CAGGGAAATT CGCAGCAGGA GCAGGCCTCC 
    TTGAGGGAAA AGATATGGAC CGGAGTGCTG GAGTGGTCAG AGAAGCCAAA GTCCGATCAA 
    CAGAAGATTC CCCATACACT CCAGTGTACC GTGTGCACCA ACATCAAAGA TGGCGAGCCC 
    GAGATCAAGG CTGAGAACTG GCCGCCCAAG CTGTTGATGC AGCTAATGCC CAAGCACCTA 
    GTTGGCAATA TTGGTGGGCA GTTTCTCAAG GACTCGAAAA TGGTTGTTTT TCGACCCACT 
    CCAGGAGAGG CACTCGATTC GCTGGCCAAG ATGATGACCT CCGGCTATGC TGGCTGCGTC 
    CACTTCTCCT CCATCCCCAA CTCACCAGCC TGTGACCTTA AGGTTCTCAT CCTACTCTAC 
    ACGCCCGATC GCAACGCTTT TCTGGGCTTC ATTCCCAATA ATCAGGCAAT GTTTGTGGAA 
    CGTCTGCGCA AAGTCATACA GCAAAAGCAG CACGGCAACA TGCAACAGCA GCAGCAACAG 
    CAGCAAATGA TGCAGCAGCA AGGCAAGTCG CCCATGGAGC TGCAGCAGCA ACAGCAACAA 
    CAACAACAGC AGCAACAGAT GCAACAGGAT AACTCGCAGC AGCAACACTA CAACCAGTTC 
    CAACTCAATA TGCAGATGGG TGGTGGAGGT CCTGGCGGCG GACCTGGTCC CGGTCCCGGC 
    GGCATGCCCA TGCAACAGAA TCAAATGCAG ATGAACATGA TGCAGCAACA GCGAATGCCA 
    CTGGGCGTAG GTGTTGGCGT GGGTGTTGGT CCCGGTGGCG TGCCCAATCC AAACCTCCAG 
    CAGCAGTTGC AACAAGTGGC GCCAAACGTC GCCGCAATGC AGCAGCAGCA GGCGCAACAG 
    CAGCAGCAGC GCATGGTGCG TCCCATGATG AGCAACAACA ACCCGGGGCT GCGCCAGCTC 
    TTGCAGCACC AGACCACGCC AGGCAATCAG TTCCGGCCGC AAATGGGTGG CCAGAATCCC 
    AATCAAATGG GAGCAGGCGG TCCAATGGTC GGCAATCGCA ACTTTGACGA CGGAAACTAT 
    GAGTTCATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_650854.1
    CDS114..2705
    Translation

    Target ORF information:

    RefSeq Version NM_142597.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mediator complex subunit 25 (MED25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_142597.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGGTGG ACCAGATTCC CCTGGCCGAC GTGGTCTTCG TCATCGAGGG CAGCGCCATC 
    AACGGAGCCT ACATCAACGA ACTAAAGACC AACTACATCC TGCCGACTTT GGAGCACTTC 
    ACCACTGGCT CCATCGACGA GCGGGAATAC CTGATTGCGG AGCGCTTTGC CACTCTGTAC 
    GGTATTGTGG TCTACCGTAC AGCGGCCAAT CTGCTGGAGC CAGTTTGTTC AACCTATGGT 
    CCCTTTCTGC AGCCCCAAAA AGTGATGGAG ACGATCGAAA GATTGCCACT AGTGGGAGGC 
    GGAATGGAGT CCTGTGCCCA CATGGCCGAA GGATTTGCTG CGGCCCACGG CTGTTTTGAT 
    GACATCAGCG AAAGGCGCCA GTTGCTCGAT CAGACCAGCG TCCAGAGGCA CTGCATTCTC 
    ATCTGCAACA GTCCGCCGTA CCAGATGCCC ACAACGGAGT CGTGGAAGTA TCCGGGCAAA 
    TCATGTGAGC AGCTGGCGGC GCTCTTCAAC GAGCGCAAAA TCAATCTCTC CATTATTGCG 
    CCACGCAAGA TGCCGGTGTT GTTCAAACTT TTCATGAAGG CCGACGGAGA TCAGCCCATT 
    ACGAGCAAAA ACTATGCCAA GAACATAAGA CACCTAGTGC TTCTAAAGGG ATACAGCCTC 
    AAGGAGAGAG CACCTAGTCC CAACAGCATG GCGGCCCAAA TGGCTGCACC CAATGCGGCA 
    CAGGCTACAG TGCAGCAGCA ACAACAACAG CAACAAAATC AAGCTGGTCA GCAACAACAA 
    GGGCAGGGCA TGCCCATGGA CACCACTCCA GCCCAGCAGC AGCAACAGCA ACAACAACAG 
    CAGCAGCAAC AGCAACAACA AGGCAATCCT CAGCAGCAGG TCATGAACAT GAACACGATG 
    CAGCAACAGC AGCCCGGTCC AAATCCCCCA GCGGGCTTAC TCAATCCACA GCAGCAGCAG 
    CAACTATTGC AGCAGCAGCA GCAGAATCAA TTCGTTTCCA ATCAAATGCA GAATCAGAAC 
    TTCCAGCAAA ACGTGGGACC CGGCCAAAAC CGCTGGATGT ATCCCAACCA GCCTGGTCAG 
    GCGCGTCCGC CCTTCATGCA GGGAGCAGGC AATGTGGGCG GTGTGGGCCA AGGCGGCGGC 
    ATGCAACAGA ATCCAAATTC TGCTCTGATA TCGCGCATTA ATGCGCCGCC GCCGAACCAA 
    ACTGTGACCT CGCTGCAGCA GCAGCAACAG CAGCAGGCGC AGCAGCAGCA GCAGCAGCAA 
    CAACAGGCCC AACAGCAGCA GCAACAAAGG ATGCAGATGC TGAGCCAGCA GCAGATGCTA 
    AATCACCAGC AGTTGCAGCA GCAACAGCAA CTGGCCCAGC AACAGCAACA GCAGCAGCAA 
    GGACAGCAAC AGCAGCAGGT AAATCCCAAT GCAGGAAACA ATATGATGCC GGCTAGCAAT 
    GCCGGCAACA TGTCTAATCC GCAGCAGCAG CAACAACAAG TAGGACAACA GTCGAATCCG 
    CAGCAGCAGG GCAATCCGCA GCAGCAACAG CAGGGAAATT CGCAGCAGGA GCAGGCCTCC 
    TTGAGGGAAA AGATATGGAC CGGAGTGCTG GAGTGGTCAG AGAAGCCAAA GTCCGATCAA 
    CAGAAGATTC CCCATACACT CCAGTGTACC GTGTGCACCA ACATCAAAGA TGGCGAGCCC 
    GAGATCAAGG CTGAGAACTG GCCGCCCAAG CTGTTGATGC AGCTAATGCC CAAGCACCTA 
    GTTGGCAATA TTGGTGGGCA GTTTCTCAAG GACTCGAAAA TGGTTGTTTT TCGACCCACT 
    CCAGGAGAGG CACTCGATTC GCTGGCCAAG ATGATGACCT CCGGCTATGC TGGCTGCGTC 
    CACTTCTCCT CCATCCCCAA CTCACCAGCC TGTGACCTTA AGGTTCTCAT CCTACTCTAC 
    ACGCCCGATC GCAACGCTTT TCTGGGCTTC ATTCCCAATA ATCAGGCAAT GTTTGTGGAA 
    CGTCTGCGCA AAGTCATACA GCAAAAGCAG CACGGCAACA TGCAACAGCA GCAGCAACAG 
    CAGCAAATGA TGCAGCAGCA AGGCAAGTCG CCCATGGAGC TGCAGCAGCA ACAGCAACAA 
    CAACAACAGC AGCAACAGAT GCAACAGGAT AACTCGCAGC AGCAACACTA CAACCAGTTC 
    CAACTCAATA TGCAGATGGG TGGTGGAGGT CCTGGCGGCG GACCTGGTCC CGGTCCCGGC 
    GGCATGCCCA TGCAACAGAA TCAAATGCAG ATGAACATGA TGCAGCAACA GCGAATGCCA 
    CTGGGCGTAG GTGTTGGCGT GGGTGTTGGT CCCGGTGGCG TGCCCAATCC AAACCTCCAG 
    CAGCAGTTGC AACAAGTGGC GCCAAACGTC GCCGCAATGC AGCAGCAGCA GGCGCAACAG 
    CAGCAGCAGC GCATGGTGCG TCCCATGATG AGCAACAACA ACCCGGGGCT GCGCCAGCTC 
    TTGCAGCACC AGACCACGCC AGGCAATCAG TTCCGGCCGC AAATGGGTGG CCAGAATCCC 
    AATCAAATGG GAGCAGGCGG TCCAATGGTC GGCAATCGCA ACTTTGACGA CGGAAACTAT 
    GAGTTCATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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