Spps cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spps
Entrez Gene ID	42882
Full Name	Sp1-like factor for pairing sensitive-silencing
Synonyms	CG5669,Dmel\CG5669
General protein information	Preferred Names Sp1-like factor for pairing sensitive-silencing Names CG5669 gene product from transcript CG5669-RA CG5669-PA CG5669-PB Sp1-like factor for pairing Sensitive-silencing Spps-PA Spps-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95F2-95F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_142975.3	NP_651232.1	Sp1-like factor for pairing sensitive-silencing, isoform A
NM_001275973.1	NP_001262902.1	Sp1-like factor for pairing sensitive-silencing, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spps, a Drosophila Sp1/KLF family member, binds to PREs and is required for PRE activity late in development
Title: Spps, a Drosophila Sp1/KLF family member, binds to PREs and is required for PRE activity late in development.

The following Spps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05921	NM_142975.3 Latest version!	Drosophila melanogaster Sp1-like factor for pairing sensitive-silencing (Spps), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa08729	NM_001275973.1 Latest version!	Drosophila melanogaster Sp1-like factor for pairing sensitive-silencing (Spps), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spps ( NM_142975.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651232.1 Drosophila melanogaster Sp1-like factor for pairing sensitive-silencing (Spps), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05921
Clone ID Related Accession (Same CDS sequence)	NM_142975.3
Accession Version	NM_142975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sp1-like factor for pairing sensitive-silencing, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142975.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCAGT ACAAGAGTGC CGCCAGTGCG GCGACTAGTG CGCCACCCGC CACGCCTTTG 
GCGGCAGGCG GTTCCAGCAA GGCGACGATT CCACCAAATG GAGCTAGTGC AGCAGCTTCA 
CAAACGCAGG TTCACGGTGC TCCCGGGACA CCAACCATGC AGCAGATCAT CAACATTCAC 
CAAATGCCTC CTCAGTTTGC AGGCGGAGCG GTTGCCGGGA ACGGACAGAA CGCTGGCATG 
CCCCAAAACA TGTTCCAGAT CGTACAGCCC ATGCCCATGC AAACGGTGAA CATTGATGGC 
CAGGAGGCCA TCTTCATACC AAACCTCAAT GCACAACTGG CCACCGCTCA GGCGGTCAAC 
TTCAACGGGC AACAGGCCTT TATCACGCCC AACGGTCAAA TTCTGCGGGC TCCCCAGATG 
GCAGCTAATC CGGCAGCCTC TAACTGCATT CAGCTGCAAC AGCTGAACGG CTTGGGCCAG 
GAGCAAACCC AGCTGATCAC CATACCCGGC ACCAACATTC AAATACCCGT CACCAATCTC 
ATTCAGCAAC AACAGCAGGC ACAACAAGTG CACCAGGGTA CAGTGCAACA GCAGGCACAA 
GGAACAAATG CATCTGGAGC CAATGGATCG GGAGTTACCA ATAGTGGGAC AGCGGGTCAG 
CTGCCGGGCA GCATCACCAT ACCGGGCACA AACCTGCAGA TACCAACCTC GGTAGCGGCG 
GCGAATGGAT TGCTGGGAAA CATCTCCAAT ATCTCCAATT TACTAGGCGG CGGGCAATCT 
ATAAAGTTGG AGAACGGCCA ATTGCAGATG CGGCCGCAAC TGGTGCAGTT TCCGGCGCCA 
GCCATGCCGC AGCAGCAACA AACGGTTGCT GTGCAGATTC CCGTTCAGAC TGCTAACGGA 
CAGACCATCT ACCAGACTGT ACATGTGCCC GTCCAGGCAG CGGCAACATC GAGCGGCGGA 
CTGCAAAACC TGATGCAGGC GCAATCCCTG CAGATGCCCT CCGCCTCGCA GATGCAAATC 
ATCCCGCAGT TCTCACAGAT AGCCCAGATT GTTACGCCCA ACGGTCAGAT TCAGCAGGTG 
CAACTGGCAA TGCCGTATCC TCAGCTGCCT CCGAATGCCA ACATTATACA TATCCAGAAT 
CCCCACCAGC AGCAGCAACA ACAAGTACAA CAACAACAAC AGCAGCAGCA GGCGCAACAG 
CAGCAGCAAG CGCAACAGCA ACAGGCACAG CAGCAGCAAC AACAACAGGT CCAGGCGCAG 
CACCAGCAGC TCCTTCAGGC GATCAGCGAT GCCTCGGCGG GGGGACAACT GCCGCCCAAT 
CAGCCCATCA CCATTACCAA TGCCCAGGGC CAACAGCTGA CCGTGATTCC CGCCCAACTA 
CGCCCAAATG CGCCTACCGC ACCCACTCCT GCTCCAGCTG GTGTGCCCAC ACCAATGCAG 
ATGCCCAATC TTCAGGCTTT ACCCATCCAG AACATCCCAG GCCTGGGTCA AGTGCAAATC 
ATTCACGCCA ATCAGCTGCC TCCTAATCTG CCAGCCAACT TCCAGCAAGT ACTAACCCAA 
CTACCCATGT CGCATCCTCA AGTGCAAACT CAGGGCCAAG TTCAGGTGAT GCCCAAGCAG 
GAGCCGCAGA GTCCAACGCA AATGATCACC AGCATCAAGC AAGAGCCGCC GGATACCTTT 
GGACCCATTT CCGCAACTGG TAATCCTCCG GCACCTGCCT CTACTCCAAA CACAGCCTCA 
CCGCAACAGC AGCAGATCAA GTTCCTGCAT ACGGAAAGCA ATTCCCTGTC CAGCCTGAGC 
ATACCAGCCT CCATTCAGAT CACAGCCCTG CCACAGCAAG CGACGAATAC ACCGAATACC 
CCAGCCACAA CCCAACCAAT TCCCGTATCG TTACCTGCAA GAAGTAAGGT CAACGCTGTG 
ACCACGTCAA GCACCCAGAT TACAATTGCG CCGACTGGTG GCCAAGTGGT GTCCGTCACG 
ACGCAGGCTA GGGGAGCAAC TGCCAGCATA AGGAGCACGA ACACCAGCAC GACGACTATA 
ACAACGCCGT CACAAAGCCA TCTCAATATG AACATAAGTG TGGCCAGCGT CGGAGGTGCT 
GCAACTGGCG GCGGCGGTGG AACAGCGACT GGAGAGCCCA AACCACGCTT AAAGCGAGTA 
GCCTGCACCT GTCCCAATTG TACAGATGGC GAAAAACACT CGGACAAGAA GCGCCAGCAT 
ATATGCCATA TAACCGGCTG CCATAAGGTA TACGGGAAAA CTTCCCATCT AAGGGCTCAC 
CTGCGTTGGC ATACTGGCGA GCGACCATTT GTCTGCTCCT GGGCGTTTTG CGGCAAGCGC 
TTCACCCGCT CCGATGAACT GCAGCGCCAC CGACGAACGC ACACGGGAGA GAAGCGTTTC 
CAGTGCCAGG AGTGCAACAA AAAGTTCATG CGCAGCGATC ACCTGTCGAA GCACATCAAG 
ACGCACTTTA AGAGCCGCTC TGGCGTGGAG CTAATTGAGC TGAGCATCAA GCAGGAGACC 
AAGGGTGGCA ATGCACCGAA AAGCATTAGC ACGGTGAACG GCATTGTGAC GATTGAGATT 
CCGGGCGGCG GTTCAGCGGC GGCGGGCAGT GGAGCCTCCA GTGTGGCGGC CACGGTTGCA 
GGCTCAACTG TGACGCCGGG CGGGGCCACG ATCGTTCAGC TGCCGACTGT GGAGGCCAGC 
GGCGGGGGTG ATAGTTTCGG CGATGATGAG GACGACGAGG AGGATGATGA ATTGACCGAA 
GAGGACGACG AAGACGAGGA GCTCGACGAG GACGATATGG ATGATTGCGA GGATGAGGAG 
GATGACGAGG ATCCAGAACT AGACAGTGGC GATGAAGTGA AGATGACAAT TGCGGTCAGT 
GAGCCTGGCG ACAATTCGTC CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651232.1
CDS	280..3186
Translation	MLQYKSAASAATSAPPATPLAAGGSSKATIPPNGASAAASQTQVHGAPGTPTMQQIINIHQMPPQFAGGAVAGNGQNAGMPQNMFQIVQPMPMQTVNIDGQEAIFIPNLNAQLATAQAVNFNGQQAFITPNGQILRAPQMAANPAASNCIQLQQLNGLGQEQTQLITIPGTNIQIPVTNLIQQQQQAQQVHQGTVQQQAQGTNASGANGSGVTNSGTAGQLPGSITIPGTNLQIPTSVAAANGLLGNISNISNLLGGGQSIKLENGQLQMRPQLVQFPAPAMPQQQQTVAVQIPVQTANGQTIYQTVHVPVQAAATSSGGLQNLMQAQSLQMPSASQMQIIPQFSQIAQIVTPNGQIQQVQLAMPYPQLPPNANIIHIQNPHQQQQQQVQQQQQQQQAQQQQQAQQQQAQQQQQQQVQAQHQQLLQAISDASAGGQLPPNQPITITNAQGQQLTVIPAQLRPNAPTAPTPAPAGVPTPMQMPNLQALPIQNIPGLGQVQIIHANQLPPNLPANFQQVLTQLPMSHPQVQTQGQVQVMPKQEPQSPTQMITSIKQEPPDTFGPISATGNPPAPASTPNTASPQQQQIKFLHTESNSLSSLSIPASIQITALPQQATNTPNTPATTQPIPVSLPARSKVNAVTTSSTQITIAPTGGQVVSVTTQARGATASIRSTNTSTTTITTPSQSHLNMNISVASVGGAATGGGGGTATGEPKPRLKRVACTCPNCTDGEKHSDKKRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKRFTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFKSRSGVELIELSIKQETKGGNAPKSISTVNGIVTIEIPGGGSAAAGSGASSVAATVAGSTVTPGGATIVQLPTVEASGGGDSFGDDEDDEEDDELTEEDDEDEELDEDDMDDCEDEEDDEDPELDSGDEVKMTIAVSEPGDNSSN

Target ORF information:

RefSeq Version	NM_142975.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sp1-like factor for pairing sensitive-silencing (Spps), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142975.3

ORF Insert Sequence:

ATGCTGCAGT ACAAGAGTGC CGCCAGTGCG GCGACTAGTG CGCCACCCGC CACGCCTTTG 
GCGGCAGGCG GTTCCAGCAA GGCGACGATT CCACCAAATG GAGCTAGTGC AGCAGCTTCA 
CAAACGCAGG TTCACGGTGC TCCCGGGACA CCAACCATGC AGCAGATCAT CAACATTCAC 
CAAATGCCTC CTCAGTTTGC AGGCGGAGCG GTTGCCGGGA ACGGACAGAA CGCTGGCATG 
CCCCAAAACA TGTTCCAGAT CGTACAGCCC ATGCCCATGC AAACGGTGAA CATTGATGGC 
CAGGAGGCCA TCTTCATACC AAACCTCAAT GCACAACTGG CCACCGCTCA GGCGGTCAAC 
TTCAACGGGC AACAGGCCTT TATCACGCCC AACGGTCAAA TTCTGCGGGC TCCCCAGATG 
GCAGCTAATC CGGCAGCCTC TAACTGCATT CAGCTGCAAC AGCTGAACGG CTTGGGCCAG 
GAGCAAACCC AGCTGATCAC CATACCCGGC ACCAACATTC AAATACCCGT CACCAATCTC 
ATTCAGCAAC AACAGCAGGC ACAACAAGTG CACCAGGGTA CAGTGCAACA GCAGGCACAA 
GGAACAAATG CATCTGGAGC CAATGGATCG GGAGTTACCA ATAGTGGGAC AGCGGGTCAG 
CTGCCGGGCA GCATCACCAT ACCGGGCACA AACCTGCAGA TACCAACCTC GGTAGCGGCG 
GCGAATGGAT TGCTGGGAAA CATCTCCAAT ATCTCCAATT TACTAGGCGG CGGGCAATCT 
ATAAAGTTGG AGAACGGCCA ATTGCAGATG CGGCCGCAAC TGGTGCAGTT TCCGGCGCCA 
GCCATGCCGC AGCAGCAACA AACGGTTGCT GTGCAGATTC CCGTTCAGAC TGCTAACGGA 
CAGACCATCT ACCAGACTGT ACATGTGCCC GTCCAGGCAG CGGCAACATC GAGCGGCGGA 
CTGCAAAACC TGATGCAGGC GCAATCCCTG CAGATGCCCT CCGCCTCGCA GATGCAAATC 
ATCCCGCAGT TCTCACAGAT AGCCCAGATT GTTACGCCCA ACGGTCAGAT TCAGCAGGTG 
CAACTGGCAA TGCCGTATCC TCAGCTGCCT CCGAATGCCA ACATTATACA TATCCAGAAT 
CCCCACCAGC AGCAGCAACA ACAAGTACAA CAACAACAAC AGCAGCAGCA GGCGCAACAG 
CAGCAGCAAG CGCAACAGCA ACAGGCACAG CAGCAGCAAC AACAACAGGT CCAGGCGCAG 
CACCAGCAGC TCCTTCAGGC GATCAGCGAT GCCTCGGCGG GGGGACAACT GCCGCCCAAT 
CAGCCCATCA CCATTACCAA TGCCCAGGGC CAACAGCTGA CCGTGATTCC CGCCCAACTA 
CGCCCAAATG CGCCTACCGC ACCCACTCCT GCTCCAGCTG GTGTGCCCAC ACCAATGCAG 
ATGCCCAATC TTCAGGCTTT ACCCATCCAG AACATCCCAG GCCTGGGTCA AGTGCAAATC 
ATTCACGCCA ATCAGCTGCC TCCTAATCTG CCAGCCAACT TCCAGCAAGT ACTAACCCAA 
CTACCCATGT CGCATCCTCA AGTGCAAACT CAGGGCCAAG TTCAGGTGAT GCCCAAGCAG 
GAGCCGCAGA GTCCAACGCA AATGATCACC AGCATCAAGC AAGAGCCGCC GGATACCTTT 
GGACCCATTT CCGCAACTGG TAATCCTCCG GCACCTGCCT CTACTCCAAA CACAGCCTCA 
CCGCAACAGC AGCAGATCAA GTTCCTGCAT ACGGAAAGCA ATTCCCTGTC CAGCCTGAGC 
ATACCAGCCT CCATTCAGAT CACAGCCCTG CCACAGCAAG CGACGAATAC ACCGAATACC 
CCAGCCACAA CCCAACCAAT TCCCGTATCG TTACCTGCAA GAAGTAAGGT CAACGCTGTG 
ACCACGTCAA GCACCCAGAT TACAATTGCG CCGACTGGTG GCCAAGTGGT GTCCGTCACG 
ACGCAGGCTA GGGGAGCAAC TGCCAGCATA AGGAGCACGA ACACCAGCAC GACGACTATA 
ACAACGCCGT CACAAAGCCA TCTCAATATG AACATAAGTG TGGCCAGCGT CGGAGGTGCT 
GCAACTGGCG GCGGCGGTGG AACAGCGACT GGAGAGCCCA AACCACGCTT AAAGCGAGTA 
GCCTGCACCT GTCCCAATTG TACAGATGGC GAAAAACACT CGGACAAGAA GCGCCAGCAT 
ATATGCCATA TAACCGGCTG CCATAAGGTA TACGGGAAAA CTTCCCATCT AAGGGCTCAC 
CTGCGTTGGC ATACTGGCGA GCGACCATTT GTCTGCTCCT GGGCGTTTTG CGGCAAGCGC 
TTCACCCGCT CCGATGAACT GCAGCGCCAC CGACGAACGC ACACGGGAGA GAAGCGTTTC 
CAGTGCCAGG AGTGCAACAA AAAGTTCATG CGCAGCGATC ACCTGTCGAA GCACATCAAG 
ACGCACTTTA AGAGCCGCTC TGGCGTGGAG CTAATTGAGC TGAGCATCAA GCAGGAGACC 
AAGGGTGGCA ATGCACCGAA AAGCATTAGC ACGGTGAACG GCATTGTGAC GATTGAGATT 
CCGGGCGGCG GTTCAGCGGC GGCGGGCAGT GGAGCCTCCA GTGTGGCGGC CACGGTTGCA 
GGCTCAACTG TGACGCCGGG CGGGGCCACG ATCGTTCAGC TGCCGACTGT GGAGGCCAGC 
GGCGGGGGTG ATAGTTTCGG CGATGATGAG GACGACGAGG AGGATGATGA ATTGACCGAA 
GAGGACGACG AAGACGAGGA GCTCGACGAG GACGATATGG ATGATTGCGA GGATGAGGAG 
GATGACGAGG ATCCAGAACT AGACAGTGGC GATGAAGTGA AGATGACAAT TGCGGTCAGT 
GAGCCTGGCG ACAATTCGTC CAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spps ( NM_001275973.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262902.1 Drosophila melanogaster Sp1-like factor for pairing sensitive-silencing (Spps), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08729
Clone ID Related Accession (Same CDS sequence)	NM_001275973.1
Accession Version	NM_001275973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sp1-like factor for pairing sensitive-silencing, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCAGT ACAAGAGTGC CGCCAGTGCG GCGACTAGTG CGCCACCCGC CACGCCTTTG 
GCGGCAGGCG GTTCCAGCAA GGCGACGATT CCACCAAATG GAGCTAGTGC AGCAGCTTCA 
CAAACGCAGG TTCACGGTGC TCCCGGGACA CCAACCATGC AGCAGATCAT CAACATTCAC 
CAAATGCCTC CTCAGTTTGC AGGCGGAGCG GTTGCCGGGA ACGGACAGAA CGCTGGCATG 
CCCCAAAACA TGTTCCAGAT CGTACAGCCC ATGCCCATGC AAACGGTGAA CATTGATGGC 
CAGGAGGCCA TCTTCATACC AAACCTCAAT GCACAACTGG CCACCGCTCA GGCGGTCAAC 
TTCAACGGGC AACAGGCCTT TATCACGCCC AACGGTCAAA TTCTGCGGGC TCCCCAGATG 
GCAGCTAATC CGGCAGCCTC TAACTGCATT CAGCTGCAAC AGCTGAACGG CTTGGGCCAG 
GAGCAAACCC AGCTGATCAC CATACCCGGC ACCAACATTC AAATACCCGT CACCAATCTC 
ATTCAGCAAC AACAGCAGGC ACAACAAGTG CACCAGGGTA CAGTGCAACA GCAGGCACAA 
GGAACAAATG CATCTGGAGC CAATGGATCG GGAGTTACCA ATAGTGGGAC AGCGGGTCAG 
CTGCCGGGCA GCATCACCAT ACCGGGCACA AACCTGCAGA TACCAACCTC GGTAGCGGCG 
GCGAATGGAT TGCTGGGAAA CATCTCCAAT ATCTCCAATT TACTAGGCGG CGGGCAATCT 
ATAAAGTTGG AGAACGGCCA ATTGCAGATG CGGCCGCAAC TGGTGCAGTT TCCGGCGCCA 
GCCATGCCGC AGCAGCAACA AACGGTTGCT GTGCAGATTC CCGTTCAGAC TGCTAACGGA 
CAGACCATCT ACCAGACTGT ACATGTGCCC GTCCAGGCAG CGGCAACATC GAGCGGCGGA 
CTGCAAAACC TGATGCAGGC GCAATCCCTG CAGATGCCCT CCGCCTCGCA GATGCAAATC 
ATCCCGCAGT TCTCACAGAT AGCCCAGATT GTTACGCCCA ACGGTCAGAT TCAGCAGGTG 
CAACTGGCAA TGCCGTATCC TCAGCTGCCT CCGAATGCCA ACATTATACA TATCCAGAAT 
CCCCACCAGC AGCAGCAACA ACAAGTACAA CAACAACAAC AGCAGCAGCA GGCGCAACAG 
CAGCAGCAAG CGCAACAGCA ACAGGCACAG CAGCAGCAAC AACAACAGGT CCAGGCGCAG 
CACCAGCAGC TCCTTCAGGC GATCAGCGAT GCCTCGGCGG GGGGACAACT GCCGCCCAAT 
CAGCCCATCA CCATTACCAA TGCCCAGGGC CAACAGCTGA CCGTGATTCC CGCCCAACTA 
CGCCCAAATG CGCCTACCGC ACCCACTCCT GCTCCAGCTG GTGTGCCCAC ACCAATGCAG 
ATGCCCAATC TTCAGGCTTT ACCCATCCAG AACATCCCAG GCCTGGGTCA AGTGCAAATC 
ATTCACGCCA ATCAGCTGCC TCCTAATCTG CCAGCCAACT TCCAGCAAGT ACTAACCCAA 
CTACCCATGT CGCATCCTCA AGTGCAAACT CAGGGCCAAG TTCAGGTGAT GCCCAAGCAG 
GAGCCGCAGA GTCCAACGCA AATGATCACC AGCATCAAGC AAGAGCCGCC GGATACCTTT 
GGACCCATTT CCGCAACTGG TAATCCTCCG GCACCTGCCT CTACTCCAAA CACAGCCTCA 
CCGCAACAGC AGCAGATCAA GTTCCTGCAT ACGGAAAGCA ATTCCCTGTC CAGCCTGAGC 
ATACCAGCCT CCATTCAGAT CACAGCCCTG CCACAGCAAG CGACGAATAC ACCGAATACC 
CCAGCCACAA CCCAACCAAT TCCCGTATCG TTACCTGCAA GAAGTAAGGT CAACGCTGTG 
ACCACGTCAA GCACCCAGAT TACAATTGCG CCGACTGGTG GCCAAGTGGT GTCCGTCACG 
ACGCAGGCTA GGGGAGCAAC TGCCAGCATA AGGAGCACGA ACACCAGCAC GACGACTATA 
ACAACGCCGT CACAAAGCCA TCTCAATATG AACATAAGTG TGGCCAGCGT CGGAGGTGCT 
GCAACTGGCG GCGGCGGTGG AACAGCGACT GGAGAGCCCA AACCACGCTT AAAGCGAGTA 
GCCTGCACCT GTCCCAATTG TACAGATGGC GAAAAACACT CGGACAAGAA GCGCCAGCAT 
ATATGCCATA TAACCGGCTG CCATAAGGTA TACGGGAAAA CTTCCCATCT AAGGGCTCAC 
CTGCGTTGGC ATACTGGCGA GCGACCATTT GTCTGCTCCT GGGCGTTTTG CGGCAAGCGC 
TTCACCCGCT CCGATGAACT GCAGCGCCAC CGACGAACGC ACACGGGAGA GAAGCGTTTC 
CAGTGCCAGG AGTGCAACAA AAAGTTCATG CGCAGCGATC ACCTGTCGAA GCACATCAAG 
ACGCACTTTA AGAGCCGCTC TGGCGTGGAG CTAATTGAGC TGAGCATCAA GCAGGAGACC 
AAGGGTGGCA ATGCACCGAA AAGCATTAGC ACGGTGAACG GCATTGTGAC GATTGAGATT 
CCGGGCGGCG GTTCAGCGGC GGCGGGCAGT GGAGCCTCCA GTGTGGCGGC CACGGTTGCA 
GGCTCAACTG TGACGCCGGG CGGGGCCACG ATCGTTCAGC TGCCGACTGT GGAGGCCAGC 
GGCGGGGGTG ATAGTTTCGG CGATGATGAG GACGACGAGG AGGATGATGA ATTGACCGAA 
GAGGACGACG AAGACGAGGA GCTCGACGAG GACGATATGG ATGATTGCGA GGATGAGGAG 
GATGACGAGG ATCCAGAACT AGACAGTGGC GATGAAGTGA AGATGACAAT TGCGGTCAGT 
GAGCCTGGCG ACAATTCGTC CAACTAGCAT TCGGAGTCGC TGTTCATGGA ACAGTTCTTG 
TTTGACCACT TCCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262902.1
CDS	280..3237
Translation	MLQYKSAASAATSAPPATPLAAGGSSKATIPPNGASAAASQTQVHGAPGTPTMQQIINIHQMPPQFAGGAVAGNGQNAGMPQNMFQIVQPMPMQTVNIDGQEAIFIPNLNAQLATAQAVNFNGQQAFITPNGQILRAPQMAANPAASNCIQLQQLNGLGQEQTQLITIPGTNIQIPVTNLIQQQQQAQQVHQGTVQQQAQGTNASGANGSGVTNSGTAGQLPGSITIPGTNLQIPTSVAAANGLLGNISNISNLLGGGQSIKLENGQLQMRPQLVQFPAPAMPQQQQTVAVQIPVQTANGQTIYQTVHVPVQAAATSSGGLQNLMQAQSLQMPSASQMQIIPQFSQIAQIVTPNGQIQQVQLAMPYPQLPPNANIIHIQNPHQQQQQQVQQQQQQQQAQQQQQAQQQQAQQQQQQQVQAQHQQLLQAISDASAGGQLPPNQPITITNAQGQQLTVIPAQLRPNAPTAPTPAPAGVPTPMQMPNLQALPIQNIPGLGQVQIIHANQLPPNLPANFQQVLTQLPMSHPQVQTQGQVQVMPKQEPQSPTQMITSIKQEPPDTFGPISATGNPPAPASTPNTASPQQQQIKFLHTESNSLSSLSIPASIQITALPQQATNTPNTPATTQPIPVSLPARSKVNAVTTSSTQITIAPTGGQVVSVTTQARGATASIRSTNTSTTTITTPSQSHLNMNISVASVGGAATGGGGGTATGEPKPRLKRVACTCPNCTDGEKHSDKKRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKRFTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFKSRSGVELIELSIKQETKGGNAPKSISTVNGIVTIEIPGGGSAAAGSGASSVAATVAGSTVTPGGATIVQLPTVEASGGGDSFGDDEDDEEDDELTEEDDEDEELDEDDMDDCEDEEDDEDPELDSGDEVKMTIAVSEPGDNSSN*HSESLFMEQFLFDHFP

Target ORF information:

RefSeq Version	NM_001275973.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sp1-like factor for pairing sensitive-silencing (Spps), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275973.1

ORF Insert Sequence:

ATGCTGCAGT ACAAGAGTGC CGCCAGTGCG GCGACTAGTG CGCCACCCGC CACGCCTTTG 
GCGGCAGGCG GTTCCAGCAA GGCGACGATT CCACCAAATG GAGCTAGTGC AGCAGCTTCA 
CAAACGCAGG TTCACGGTGC TCCCGGGACA CCAACCATGC AGCAGATCAT CAACATTCAC 
CAAATGCCTC CTCAGTTTGC AGGCGGAGCG GTTGCCGGGA ACGGACAGAA CGCTGGCATG 
CCCCAAAACA TGTTCCAGAT CGTACAGCCC ATGCCCATGC AAACGGTGAA CATTGATGGC 
CAGGAGGCCA TCTTCATACC AAACCTCAAT GCACAACTGG CCACCGCTCA GGCGGTCAAC 
TTCAACGGGC AACAGGCCTT TATCACGCCC AACGGTCAAA TTCTGCGGGC TCCCCAGATG 
GCAGCTAATC CGGCAGCCTC TAACTGCATT CAGCTGCAAC AGCTGAACGG CTTGGGCCAG 
GAGCAAACCC AGCTGATCAC CATACCCGGC ACCAACATTC AAATACCCGT CACCAATCTC 
ATTCAGCAAC AACAGCAGGC ACAACAAGTG CACCAGGGTA CAGTGCAACA GCAGGCACAA 
GGAACAAATG CATCTGGAGC CAATGGATCG GGAGTTACCA ATAGTGGGAC AGCGGGTCAG 
CTGCCGGGCA GCATCACCAT ACCGGGCACA AACCTGCAGA TACCAACCTC GGTAGCGGCG 
GCGAATGGAT TGCTGGGAAA CATCTCCAAT ATCTCCAATT TACTAGGCGG CGGGCAATCT 
ATAAAGTTGG AGAACGGCCA ATTGCAGATG CGGCCGCAAC TGGTGCAGTT TCCGGCGCCA 
GCCATGCCGC AGCAGCAACA AACGGTTGCT GTGCAGATTC CCGTTCAGAC TGCTAACGGA 
CAGACCATCT ACCAGACTGT ACATGTGCCC GTCCAGGCAG CGGCAACATC GAGCGGCGGA 
CTGCAAAACC TGATGCAGGC GCAATCCCTG CAGATGCCCT CCGCCTCGCA GATGCAAATC 
ATCCCGCAGT TCTCACAGAT AGCCCAGATT GTTACGCCCA ACGGTCAGAT TCAGCAGGTG 
CAACTGGCAA TGCCGTATCC TCAGCTGCCT CCGAATGCCA ACATTATACA TATCCAGAAT 
CCCCACCAGC AGCAGCAACA ACAAGTACAA CAACAACAAC AGCAGCAGCA GGCGCAACAG 
CAGCAGCAAG CGCAACAGCA ACAGGCACAG CAGCAGCAAC AACAACAGGT CCAGGCGCAG 
CACCAGCAGC TCCTTCAGGC GATCAGCGAT GCCTCGGCGG GGGGACAACT GCCGCCCAAT 
CAGCCCATCA CCATTACCAA TGCCCAGGGC CAACAGCTGA CCGTGATTCC CGCCCAACTA 
CGCCCAAATG CGCCTACCGC ACCCACTCCT GCTCCAGCTG GTGTGCCCAC ACCAATGCAG 
ATGCCCAATC TTCAGGCTTT ACCCATCCAG AACATCCCAG GCCTGGGTCA AGTGCAAATC 
ATTCACGCCA ATCAGCTGCC TCCTAATCTG CCAGCCAACT TCCAGCAAGT ACTAACCCAA 
CTACCCATGT CGCATCCTCA AGTGCAAACT CAGGGCCAAG TTCAGGTGAT GCCCAAGCAG 
GAGCCGCAGA GTCCAACGCA AATGATCACC AGCATCAAGC AAGAGCCGCC GGATACCTTT 
GGACCCATTT CCGCAACTGG TAATCCTCCG GCACCTGCCT CTACTCCAAA CACAGCCTCA 
CCGCAACAGC AGCAGATCAA GTTCCTGCAT ACGGAAAGCA ATTCCCTGTC CAGCCTGAGC 
ATACCAGCCT CCATTCAGAT CACAGCCCTG CCACAGCAAG CGACGAATAC ACCGAATACC 
CCAGCCACAA CCCAACCAAT TCCCGTATCG TTACCTGCAA GAAGTAAGGT CAACGCTGTG 
ACCACGTCAA GCACCCAGAT TACAATTGCG CCGACTGGTG GCCAAGTGGT GTCCGTCACG 
ACGCAGGCTA GGGGAGCAAC TGCCAGCATA AGGAGCACGA ACACCAGCAC GACGACTATA 
ACAACGCCGT CACAAAGCCA TCTCAATATG AACATAAGTG TGGCCAGCGT CGGAGGTGCT 
GCAACTGGCG GCGGCGGTGG AACAGCGACT GGAGAGCCCA AACCACGCTT AAAGCGAGTA 
GCCTGCACCT GTCCCAATTG TACAGATGGC GAAAAACACT CGGACAAGAA GCGCCAGCAT 
ATATGCCATA TAACCGGCTG CCATAAGGTA TACGGGAAAA CTTCCCATCT AAGGGCTCAC 
CTGCGTTGGC ATACTGGCGA GCGACCATTT GTCTGCTCCT GGGCGTTTTG CGGCAAGCGC 
TTCACCCGCT CCGATGAACT GCAGCGCCAC CGACGAACGC ACACGGGAGA GAAGCGTTTC 
CAGTGCCAGG AGTGCAACAA AAAGTTCATG CGCAGCGATC ACCTGTCGAA GCACATCAAG 
ACGCACTTTA AGAGCCGCTC TGGCGTGGAG CTAATTGAGC TGAGCATCAA GCAGGAGACC 
AAGGGTGGCA ATGCACCGAA AAGCATTAGC ACGGTGAACG GCATTGTGAC GATTGAGATT 
CCGGGCGGCG GTTCAGCGGC GGCGGGCAGT GGAGCCTCCA GTGTGGCGGC CACGGTTGCA 
GGCTCAACTG TGACGCCGGG CGGGGCCACG ATCGTTCAGC TGCCGACTGT GGAGGCCAGC 
GGCGGGGGTG ATAGTTTCGG CGATGATGAG GACGACGAGG AGGATGATGA ATTGACCGAA 
GAGGACGACG AAGACGAGGA GCTCGACGAG GACGATATGG ATGATTGCGA GGATGAGGAG 
GATGACGAGG ATCCAGAACT AGACAGTGGC GATGAAGTGA AGATGACAAT TGCGGTCAGT 
GAGCCTGGCG ACAATTCGTC CAACTAGCAT TCGGAGTCGC TGTTCATGGA ACAGTTCTTG 
TTTGACCACT TCCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spps cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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