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HIS2AV cDNA ORF clone, Drosophila melanogaster(Fruit fly)

Gene Symbol HIS2AV
Entrez Gene ID 43229
Full Name Histone H2A variant
Synonyms 5499,CG5499,Dmel\CG5499,H2A,H2A.F/Z,H2A.V,H2A.X,H2A.Z,H2AV,H2AV_DROM,H2AX,H2AZ,H2Av,H2AvD,H2a.V,H2av,H2avD,HIS,HIS2AV,HIS2AVD,His,His2,His2A,His2AV,His2AvD,HisH2Av,Hist,Hist2av,gamma-H2AV,gamma-H2Av,gamma-HIS2AV,gamma-His2Av,gammaH2AV,gammaH2Av,h2AvD,his,his2Av,l(3)05146,l(3)810,l(3)97Dd,l(3)L1602
General protein information
Preferred Names

Histone H2A variant

Names

CG5499 gene product from transcript CG5499-RB
CG5499-PA
CG5499-PB
H2A variant
His2Av-PA
His2Av-PB
Histone-2A
Histone-2av
histone
histone 2A variant
histone H2A variant
histone H2Av
histone variant H2Av
histone2A
histone2Av
histone2AvD

Gene Type protein-coding
Organism Drosophila melanogaster(Fruit fly)
Genome

3R

97D3-97D3


mRNA and Protein(s)

mRNA Protein Name
NM_079795.3 NP_524519.1 histone H2A variant, isoform A
NM_001276068.1 NP_001262997.1 histone H2A variant, isoform B



Pan troglodytes (chimpanzee) H2AFV XP_510606.3
Kluyveromyces lactis KLLA0C05918g XP_452461.1
Arabidopsis thaliana (thale cress) HTA8 NP_850299.1
Oryza sativa (rice) Os03g0162200 NP_001049049.1
Bos taurus (cattle) H2AFV NP_001033286.1
Drosophila melanogaster (fruit fly) His2Av NP_524519.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000306 XP_310818.3
Arabidopsis thaliana (thale cress) HTA11 NP_191019.1
Oryza sativa (rice) Os03g0743400 NP_001051232.1
Rattus norvegicus (Norway rat) LOC685909 NP_001099489.1
Danio rerio (zebrafish) h2afva NP_705930.1
Macaca mulatta (rhesus monkey) LOC701962 XP_001097247.1
Mus musculus (house mouse) H2afv NP_084214.1
Caenorhabditis elegans (roundworm) htz-1 NP_500569.1
Eremothecium gossypii AGOS_ACL017C NP_983387.1
Homo sapiens (human) H2AFV NP_036544.1
Saccharomyces cerevisiae (baker's yeast) HTZ1 NP_014631.1
Schizosaccharomyces pombe (fission yeast) pht1 NP_595630.3
Neurospora crassa NCU05347 XP_963338.1
Oryza sativa (rice) Os10g0418000 NP_001064611.1
Xenopus tropicalis (tropical clawed frog) h2afv NP_001016178.1


Related articles in PubMed

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.
Hoskins RA, Smith CD, Carlson JW, Carvalho AB, Halpern A, Kaminker JS, Kennedy C, Mungall CJ, Sullivan BA, Sutton GG, Yasuhara JC, Wakimoto BT, Myers EW, Celniker SE, Rubin GM, Karpen GH
Genome biology3(12)RESEARCH0085(2002)

The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective.
Kaminker JS, Bergman CM, Kronmiller B, Carlson J, Svirskas R, Patel S, Frise E, Wheeler DA, Lewis SE, Rubin GM, Ashburner M, Celniker SE
Genome biology3(12)RESEARCH0084(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study reports the crystal structure of the H2A.Z-binding domain of Drosophila melanogaster YL1 (dYL1-Z) in complex with an H2A.Z-H2B dimer at 1.9-A resolution.
Title: Structural basis of H2A.Z recognition by SRCAP chromatin-remodeling subunit YL1.

The Drosophila histone variant H2A.V works in concert with HP1 to promote kinetochore-driven microtubule formation.
Title: The Drosophila histone variant H2A.V works in concert with HP1 to promote kinetochore-driven microtubule formation.

chromatin loosening and associated initiation of gene expression is activated by phosphorylation of H2Av in a nucleosome positioned in promoter regions of PARP-1-dependent genes
Title: Kinase-mediated changes in nucleosome conformation trigger chromatin decondensation via poly(ADP-ribosyl)ation.

nucleosomes present significant, context-specific barriers to RNAPII in vivo that can be tuned by the incorporation of H2A.Z
Title: Nucleosomes are context-specific, H2A.Z-modulated barriers to RNA polymerase.

Unlike other organisms that employ several H2A variants for different functions, the parsimonious fruit fly Drosophila melanogaster gets along with just a single H2A variant, H2A.V. Remarkably, H2A.V unites within one molecule features and functions of two different mammalian H2A variants, H2A.Z and H2A.X. [Review]
Title: The variant histone H2A.V of Drosophila--three roles, two guises.

The following HIS2AV gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIS2AV cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OFa06243 NM_001276068.1
Latest version!
Drosophila melanogaster histone H2A variant, transcript variant B (His2Av), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OFa06243 NM_079795.3
Latest version!
Drosophila melanogaster histone H2A variant, transcript variant A (His2Av), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OFa06243
Clone ID Related Accession (Same CDS sequence) NM_079795.3 , NM_001276068.1
Accession Version NM_079795.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-03-14
Organism Drosophila melanogaster(Fruit fly)
Product histone H2A variant, isoform A
Comment REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079795.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524519.1
CDS165..590
Misc Feature(1)171..560
Misc Feature(2)186..530
Misc Feature(3)order(186..188,198..200)
Misc Feature(4)order(258..260,267..269,276..281,300..302,396..398,405..407)
Misc Feature(5)order(285..293,297..299)
Misc Feature(6)order(327..329,336..341,348..353,360..365)
Misc Feature(7)528..530
Translation

Target ORF information:

RefSeq Version NM_079795.3
Organism Drosophila melanogaster(Fruit fly)
Definition Drosophila melanogaster histone H2A variant, transcript variant A (His2Av), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_079795.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa06243
Clone ID Related Accession (Same CDS sequence) NM_079795.3 , NM_001276068.1
Accession Version NM_001276068.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-03-14
Organism Drosophila melanogaster(Fruit fly)
Product histone H2A variant, isoform B
Comment REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001262997.1
CDS165..590
Misc Feature(1)171..560
Misc Feature(2)186..530
Misc Feature(3)order(186..188,198..200)
Misc Feature(4)order(258..260,267..269,276..281,300..302,396..398,405..407)
Misc Feature(5)order(285..293,297..299)
Misc Feature(6)order(327..329,336..341,348..353,360..365)
Misc Feature(7)528..530
Translation

Target ORF information:

RefSeq Version NM_001276068.1
Organism Drosophila melanogaster(Fruit fly)
Definition Drosophila melanogaster histone H2A variant, transcript variant B (His2Av), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276068.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D


book

Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.
Genome biology3(12)RESEARCH0085(2002)
Hoskins RA,Smith CD,Carlson JW,Carvalho AB,Halpern A,Kaminker JS,Kennedy C,Mungall CJ,Sullivan BA,Sutton GG,Yasuhara JC,Wakimoto BT,Myers EW,Celniker SE,Rubin GM,Karpen GH


book

The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective.
Genome biology3(12)RESEARCH0084(2002)
Kaminker JS,Bergman CM,Kronmiller B,Carlson J,Svirskas R,Patel S,Frise E,Wheeler DA,Lewis SE,Rubin GM,Ashburner M,Celniker SE


 
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