mino cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mino
Entrez Gene ID	43350
Full Name	minotaur
Synonyms	152088_at,BcDNA:GH07066,CG5508,Dmel\CG5508,GPAT1
General protein information	Preferred Names minotaur Names CG5508 gene product from transcript CG5508-RA CG5508-PA CG5508-PB CG5508-PC CG5508-PE mino-PA mino-PB mino-PC mino-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98B6-98B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170359.3	NP_733238.1	minotaur, isoform B
NM_001260414.2	NP_001247343.2	minotaur, isoform E
NM_143340.4	NP_651597.1	minotaur, isoform A
NM_170358.2	NP_733237.1	minotaur, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5508	NP_733237.1
Mus musculus (house mouse)	Gpam	NP_032175.2
Gallus gallus (chicken)	GPAM	XP_421757.2
Homo sapiens (human)	GPAM	NP_001231878.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003687	XP_313470.4
	GPAM	XP_001088103.1
Bos taurus (cattle)	GPAM	NP_001012282.1
Rattus norvegicus (Norway rat)	Gpam	NP_058970.1
Xenopus tropicalis (tropical clawed frog)	MGC145183	NP_001072564.1
Pan troglodytes (chimpanzee)	GPAM	XP_508037.2
Canis lupus familiaris (dog)	GPAM	XP_005637781.1
Danio rerio (zebrafish)	gpam	XP_002664023.2
Caenorhabditis elegans (roundworm)	acl-6	NP_001023769.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Though this points strongly to a function for phospholipid signaling in the piRNA pathway, a mutant form of CG5508, which lacks the GPAT active site, still functions in piRNA biogenesis. We have named this new biogenesis factor Minotaur.
Title: Minotaur is critical for primary piRNA biogenesis.

The following mino gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mino cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06369	NM_143340.4 Latest version!	Drosophila melanogaster minotaur (mino), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06369	NM_001260414.2 Latest version!	Drosophila melanogaster minotaur (mino), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06370	NM_170358.2 Latest version!	Drosophila melanogaster minotaur (mino), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06371	NM_170359.3 Latest version!	Drosophila melanogaster minotaur (mino), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mino ( NM_001260414.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247343.2 Drosophila melanogaster minotaur (mino), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa06369
Clone ID Related Accession (Same CDS sequence)	NM_001260414.2 , NM_143340.4
Accession Version	NM_001260414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	minotaur, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260414.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACGA CCGTCCTGAA CTTTGCCCAG CGCGTCGCCG AGATTTCTTT GCTCGGCGGC 
ATCGAGATTG CATCAGTTGC CCTTATTGTC TACTATATAT TCGCCAAGAC GAGGGCACCG 
GTAATCATCG CTAGCCTGTT TCCCAATCTG AATCTGAGCT ACCGGACGAG CTACCTCAGC 
AAGATCAACA CCATACGCAG CGATCTTCTG GTCACTTTGC GATCATCCAA TGAGCCCAAG 
ACGCAGAATT CGGTGGCTCA TGGCATTGGG ACGCAGAATA TGTTGGAGCT GACCCCGCAT 
TCGGGTCAGA AGCCTTTTAA GAAGCTATTC GACTGGGGCG TGCTCTTCCC ATATGTTGCC 
CAGGTGGTGC GCAGCGAGAA GTTCGAGTAC CGACAGGTCA CCGAGATCGT GCACAACGAT 
GCTGTACTGA AGCATGCAAT TAAACAGGCC GCCGAGCAGA CGCTAAGGGA GCAGAGATAT 
GCTAAAAATG GTCATCGTCT GCTCACGCGC AGTGCTAGTG GAGATCAGGA GGCGCAGGAG 
GAGGAGGACG AGAAGAGGGG TATATCCTAT CAGGCCATTC TGCGTAAGCA GGAGCAGCGC 
GCCATTAGCA TACTGAAGGA CATGGGCTCC ACTCTGAACA ATGGCCTTTT GGCCTTCACT 
TCCTGGATCC TGTACAAGTT GCTGCCCTGC TTCCTCTCCG GAGTCGTTAC GAACACGAAG 
CAGATCGAGA TGCTCAAAAC AGCCACAGAG CGTTCGCCGG GCACTCCGCT GATATTTGTT 
CCGCTGCATC GCAGCCACTT GGACTACATC ATGGTCACAT GGATACTCAC GAACAACGAT 
ATACGCAGTC CTCTGGTAGC CGCTGGCAAC AACCTGCAAA TTCCGGTCTT TGGTGGGCTC 
CTACGTGGTT TGGGTGCTTT CTTCATCAAA CGCAAGATTG ATCCTGTGGA GGGCAAGAAG 
GACGTGCTTT ATCGCGCTGC TCTACACCTG TATCTGACCC ACGCCTTGAA GCAGGGCCAC 
AACGTCGAGT TCTTCATCGA GGGCGGACGA ACGCGCACTG GCAAGCCGTG CATGCCCAAG 
GGCGGCATTC TTTCTGTGAT TGTGAACGCC TTCATGGACG GCAGCATACC GGACGCACTG 
CTGGTACCCG TTTCCGTAAA CTACGAGCGT CTCGTCGACG GAAACTTTGT GCGCGAACAA 
AAGGGCGAGA AGAAGATACC CGAATCATTC GGCAAGGCCA TTTCGGGCAT TTGGAAGGCC 
CTGAAATCAA ACTACGGCCT CATGCGCATC GACTTTAACG AGCCCTACTC CATACGTGAG 
CTGGTCAATT CGTACAACAA GATAGCCCGA GAAGATGGAA ACATTGCCAA GGTCTACAAG 
CCATCTGCCA GGGTCCTACA GCACAATCAG TCCACCTCCT CCTTGTACGG CACCGATGTT 
GTGTGCGAGG AGCACCGCAA TCTCATCGAA AGCATCTCGC GCCAGGTGGT CTTTGACTGC 
GCAGCAGCCA CCTCTGTGAT GTCCACCAAT GCGTTAGCCT TTTTGCTGCT CACCCGCTTT 
AGGAACGGTG CCGAGGAGCA GATTCTGTCG GAGGCTCTGG ACGACTTGCG AAACTCGTTG 
TCGGGCTGCA AGGACATTGG TTTTTCCGGG GAATCCTCGC AAATTGTGGC ATATGCCTGT 
GACCTACTCG GCTCAGGTCT TGTGACTCGC TCTCGGGATG AGAACGGGCG TTTGGTGATT 
AAGGCTGTGA ATTCAGTGGA AAGCTTTATT GAGCTGGCTT ACTATTCGAA CATGCTGACG 
CCGCACTTTG CCCTCAGCTC GATATTGTTG ACCACCTTCC ACTCACTGCT TCCGGAAACG 
GAGAATAAGA AAGAAGCCGC CGTGTCGCGC AAAAAGCTGA TAGACACCGC GCTGGAAAAC 
TGCCAGATCT ATCGCTACGA ATTTATTCTG AACAAACCGA CGCAGGTGCT GGAAAATTTG 
CTGTACCAGC AGTTGGACGA CCTCTTGATT AGTGGTTGCG TTTTGACCGA AAAGCTCCAT 
GACGATTTGC CGAATGGCGC TGAGGGCAGG CGCTTGGCCA ACGTCCTGGC AGAGTGCCTG 
GACGAGGACG GCTACGAAGA CGTGCGCGAT GGCGAGGCGG ATGAGCCCAA ACTGCTGTTC 
GCCAGCGAAA CCCCGTCGCA GCAGCGCTAC ATTTGCGAGG TGCTGGCGCC CTTCGCCTGG 
ACGTATGTGA CGGTGGCGCA GTCCCTGCAA ATACTGCACA AGAACTCCAT GCTGGAGTCT 
GAATTTATAA GCTTTGTGAT TAACGATCTG AGCAGCAAGG TCAAGCGCGG CAGCTGCATC 
TATGCCGAAA GCATATCCAC CGACTCCGTG CGCAACTGCC TGAAGCTGTT GGAGAAATGG 
TCTGTGATCG AGGTTTGCAA CCAGCAGGGC ATGCGCCTGA TCTCACTCAA CACGCTCTAC 
GAGATGTCTC GTGAGTCGCT CAACTCGATC GTGAAGAAGA TCGACGCAAT TGTACCAAAA 
TTCAACTCGG GCTACTTCAG TGTAACGCCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247343.2
CDS	217..2769
Translation	MLTTVLNFAQRVAEISLLGGIEIASVALIVYYIFAKTRAPVIIASLFPNLNLSYRTSYLSKINTIRSDLLVTLRSSNEPKTQNSVAHGIGTQNMLELTPHSGQKPFKKLFDWGVLFPYVAQVVRSEKFEYRQVTEIVHNDAVLKHAIKQAAEQTLREQRYAKNGHRLLTRSASGDQEAQEEEDEKRGISYQAILRKQEQRAISILKDMGSTLNNGLLAFTSWILYKLLPCFLSGVVTNTKQIEMLKTATERSPGTPLIFVPLHRSHLDYIMVTWILTNNDIRSPLVAAGNNLQIPVFGGLLRGLGAFFIKRKIDPVEGKKDVLYRAALHLYLTHALKQGHNVEFFIEGGRTRTGKPCMPKGGILSVIVNAFMDGSIPDALLVPVSVNYERLVDGNFVREQKGEKKIPESFGKAISGIWKALKSNYGLMRIDFNEPYSIRELVNSYNKIAREDGNIAKVYKPSARVLQHNQSTSSLYGTDVVCEEHRNLIESISRQVVFDCAAATSVMSTNALAFLLLTRFRNGAEEQILSEALDDLRNSLSGCKDIGFSGESSQIVAYACDLLGSGLVTRSRDENGRLVIKAVNSVESFIELAYYSNMLTPHFALSSILLTTFHSLLPETENKKEAAVSRKKLIDTALENCQIYRYEFILNKPTQVLENLLYQQLDDLLISGCVLTEKLHDDLPNGAEGRRLANVLAECLDEDGYEDVRDGEADEPKLLFASETPSQQRYICEVLAPFAWTYVTVAQSLQILHKNSMLESEFISFVINDLSSKVKRGSCIYAESISTDSVRNCLKLLEKWSVIEVCNQQGMRLISLNTLYEMSRESLNSIVKKIDAIVPKFNSGYFSVTP

Target ORF information:

RefSeq Version	NM_001260414.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minotaur (mino), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260414.2

ORF Insert Sequence:

ATGTTGACGA CCGTCCTGAA CTTTGCCCAG CGCGTCGCCG AGATTTCTTT GCTCGGCGGC 
ATCGAGATTG CATCAGTTGC CCTTATTGTC TACTATATAT TCGCCAAGAC GAGGGCACCG 
GTAATCATCG CTAGCCTGTT TCCCAATCTG AATCTGAGCT ACCGGACGAG CTACCTCAGC 
AAGATCAACA CCATACGCAG CGATCTTCTG GTCACTTTGC GATCATCCAA TGAGCCCAAG 
ACGCAGAATT CGGTGGCTCA TGGCATTGGG ACGCAGAATA TGTTGGAGCT GACCCCGCAT 
TCGGGTCAGA AGCCTTTTAA GAAGCTATTC GACTGGGGCG TGCTCTTCCC ATATGTTGCC 
CAGGTGGTGC GCAGCGAGAA GTTCGAGTAC CGACAGGTCA CCGAGATCGT GCACAACGAT 
GCTGTACTGA AGCATGCAAT TAAACAGGCC GCCGAGCAGA CGCTAAGGGA GCAGAGATAT 
GCTAAAAATG GTCATCGTCT GCTCACGCGC AGTGCTAGTG GAGATCAGGA GGCGCAGGAG 
GAGGAGGACG AGAAGAGGGG TATATCCTAT CAGGCCATTC TGCGTAAGCA GGAGCAGCGC 
GCCATTAGCA TACTGAAGGA CATGGGCTCC ACTCTGAACA ATGGCCTTTT GGCCTTCACT 
TCCTGGATCC TGTACAAGTT GCTGCCCTGC TTCCTCTCCG GAGTCGTTAC GAACACGAAG 
CAGATCGAGA TGCTCAAAAC AGCCACAGAG CGTTCGCCGG GCACTCCGCT GATATTTGTT 
CCGCTGCATC GCAGCCACTT GGACTACATC ATGGTCACAT GGATACTCAC GAACAACGAT 
ATACGCAGTC CTCTGGTAGC CGCTGGCAAC AACCTGCAAA TTCCGGTCTT TGGTGGGCTC 
CTACGTGGTT TGGGTGCTTT CTTCATCAAA CGCAAGATTG ATCCTGTGGA GGGCAAGAAG 
GACGTGCTTT ATCGCGCTGC TCTACACCTG TATCTGACCC ACGCCTTGAA GCAGGGCCAC 
AACGTCGAGT TCTTCATCGA GGGCGGACGA ACGCGCACTG GCAAGCCGTG CATGCCCAAG 
GGCGGCATTC TTTCTGTGAT TGTGAACGCC TTCATGGACG GCAGCATACC GGACGCACTG 
CTGGTACCCG TTTCCGTAAA CTACGAGCGT CTCGTCGACG GAAACTTTGT GCGCGAACAA 
AAGGGCGAGA AGAAGATACC CGAATCATTC GGCAAGGCCA TTTCGGGCAT TTGGAAGGCC 
CTGAAATCAA ACTACGGCCT CATGCGCATC GACTTTAACG AGCCCTACTC CATACGTGAG 
CTGGTCAATT CGTACAACAA GATAGCCCGA GAAGATGGAA ACATTGCCAA GGTCTACAAG 
CCATCTGCCA GGGTCCTACA GCACAATCAG TCCACCTCCT CCTTGTACGG CACCGATGTT 
GTGTGCGAGG AGCACCGCAA TCTCATCGAA AGCATCTCGC GCCAGGTGGT CTTTGACTGC 
GCAGCAGCCA CCTCTGTGAT GTCCACCAAT GCGTTAGCCT TTTTGCTGCT CACCCGCTTT 
AGGAACGGTG CCGAGGAGCA GATTCTGTCG GAGGCTCTGG ACGACTTGCG AAACTCGTTG 
TCGGGCTGCA AGGACATTGG TTTTTCCGGG GAATCCTCGC AAATTGTGGC ATATGCCTGT 
GACCTACTCG GCTCAGGTCT TGTGACTCGC TCTCGGGATG AGAACGGGCG TTTGGTGATT 
AAGGCTGTGA ATTCAGTGGA AAGCTTTATT GAGCTGGCTT ACTATTCGAA CATGCTGACG 
CCGCACTTTG CCCTCAGCTC GATATTGTTG ACCACCTTCC ACTCACTGCT TCCGGAAACG 
GAGAATAAGA AAGAAGCCGC CGTGTCGCGC AAAAAGCTGA TAGACACCGC GCTGGAAAAC 
TGCCAGATCT ATCGCTACGA ATTTATTCTG AACAAACCGA CGCAGGTGCT GGAAAATTTG 
CTGTACCAGC AGTTGGACGA CCTCTTGATT AGTGGTTGCG TTTTGACCGA AAAGCTCCAT 
GACGATTTGC CGAATGGCGC TGAGGGCAGG CGCTTGGCCA ACGTCCTGGC AGAGTGCCTG 
GACGAGGACG GCTACGAAGA CGTGCGCGAT GGCGAGGCGG ATGAGCCCAA ACTGCTGTTC 
GCCAGCGAAA CCCCGTCGCA GCAGCGCTAC ATTTGCGAGG TGCTGGCGCC CTTCGCCTGG 
ACGTATGTGA CGGTGGCGCA GTCCCTGCAA ATACTGCACA AGAACTCCAT GCTGGAGTCT 
GAATTTATAA GCTTTGTGAT TAACGATCTG AGCAGCAAGG TCAAGCGCGG CAGCTGCATC 
TATGCCGAAA GCATATCCAC CGACTCCGTG CGCAACTGCC TGAAGCTGTT GGAGAAATGG 
TCTGTGATCG AGGTTTGCAA CCAGCAGGGC ATGCGCCTGA TCTCACTCAA CACGCTCTAC 
GAGATGTCTC GTGAGTCGCT CAACTCGATC GTGAAGAAGA TCGACGCAAT TGTACCAAAA 
TTCAACTCGG GCTACTTCAG TGTAACGCCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mino ( NM_143340.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651597.1 Drosophila melanogaster minotaur (mino), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06369
Clone ID Related Accession (Same CDS sequence)	NM_001260414.2 , NM_143340.4
Accession Version	NM_143340.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	minotaur, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143340.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACGA CCGTCCTGAA CTTTGCCCAG CGCGTCGCCG AGATTTCTTT GCTCGGCGGC 
ATCGAGATTG CATCAGTTGC CCTTATTGTC TACTATATAT TCGCCAAGAC GAGGGCACCG 
GTAATCATCG CTAGCCTGTT TCCCAATCTG AATCTGAGCT ACCGGACGAG CTACCTCAGC 
AAGATCAACA CCATACGCAG CGATCTTCTG GTCACTTTGC GATCATCCAA TGAGCCCAAG 
ACGCAGAATT CGGTGGCTCA TGGCATTGGG ACGCAGAATA TGTTGGAGCT GACCCCGCAT 
TCGGGTCAGA AGCCTTTTAA GAAGCTATTC GACTGGGGCG TGCTCTTCCC ATATGTTGCC 
CAGGTGGTGC GCAGCGAGAA GTTCGAGTAC CGACAGGTCA CCGAGATCGT GCACAACGAT 
GCTGTACTGA AGCATGCAAT TAAACAGGCC GCCGAGCAGA CGCTAAGGGA GCAGAGATAT 
GCTAAAAATG GTCATCGTCT GCTCACGCGC AGTGCTAGTG GAGATCAGGA GGCGCAGGAG 
GAGGAGGACG AGAAGAGGGG TATATCCTAT CAGGCCATTC TGCGTAAGCA GGAGCAGCGC 
GCCATTAGCA TACTGAAGGA CATGGGCTCC ACTCTGAACA ATGGCCTTTT GGCCTTCACT 
TCCTGGATCC TGTACAAGTT GCTGCCCTGC TTCCTCTCCG GAGTCGTTAC GAACACGAAG 
CAGATCGAGA TGCTCAAAAC AGCCACAGAG CGTTCGCCGG GCACTCCGCT GATATTTGTT 
CCGCTGCATC GCAGCCACTT GGACTACATC ATGGTCACAT GGATACTCAC GAACAACGAT 
ATACGCAGTC CTCTGGTAGC CGCTGGCAAC AACCTGCAAA TTCCGGTCTT TGGTGGGCTC 
CTACGTGGTT TGGGTGCTTT CTTCATCAAA CGCAAGATTG ATCCTGTGGA GGGCAAGAAG 
GACGTGCTTT ATCGCGCTGC TCTACACCTG TATCTGACCC ACGCCTTGAA GCAGGGCCAC 
AACGTCGAGT TCTTCATCGA GGGCGGACGA ACGCGCACTG GCAAGCCGTG CATGCCCAAG 
GGCGGCATTC TTTCTGTGAT TGTGAACGCC TTCATGGACG GCAGCATACC GGACGCACTG 
CTGGTACCCG TTTCCGTAAA CTACGAGCGT CTCGTCGACG GAAACTTTGT GCGCGAACAA 
AAGGGCGAGA AGAAGATACC CGAATCATTC GGCAAGGCCA TTTCGGGCAT TTGGAAGGCC 
CTGAAATCAA ACTACGGCCT CATGCGCATC GACTTTAACG AGCCCTACTC CATACGTGAG 
CTGGTCAATT CGTACAACAA GATAGCCCGA GAAGATGGAA ACATTGCCAA GGTCTACAAG 
CCATCTGCCA GGGTCCTACA GCACAATCAG TCCACCTCCT CCTTGTACGG CACCGATGTT 
GTGTGCGAGG AGCACCGCAA TCTCATCGAA AGCATCTCGC GCCAGGTGGT CTTTGACTGC 
GCAGCAGCCA CCTCTGTGAT GTCCACCAAT GCGTTAGCCT TTTTGCTGCT CACCCGCTTT 
AGGAACGGTG CCGAGGAGCA GATTCTGTCG GAGGCTCTGG ACGACTTGCG AAACTCGTTG 
TCGGGCTGCA AGGACATTGG TTTTTCCGGG GAATCCTCGC AAATTGTGGC ATATGCCTGT 
GACCTACTCG GCTCAGGTCT TGTGACTCGC TCTCGGGATG AGAACGGGCG TTTGGTGATT 
AAGGCTGTGA ATTCAGTGGA AAGCTTTATT GAGCTGGCTT ACTATTCGAA CATGCTGACG 
CCGCACTTTG CCCTCAGCTC GATATTGTTG ACCACCTTCC ACTCACTGCT TCCGGAAACG 
GAGAATAAGA AAGAAGCCGC CGTGTCGCGC AAAAAGCTGA TAGACACCGC GCTGGAAAAC 
TGCCAGATCT ATCGCTACGA ATTTATTCTG AACAAACCGA CGCAGGTGCT GGAAAATTTG 
CTGTACCAGC AGTTGGACGA CCTCTTGATT AGTGGTTGCG TTTTGACCGA AAAGCTCCAT 
GACGATTTGC CGAATGGCGC TGAGGGCAGG CGCTTGGCCA ACGTCCTGGC AGAGTGCCTG 
GACGAGGACG GCTACGAAGA CGTGCGCGAT GGCGAGGCGG ATGAGCCCAA ACTGCTGTTC 
GCCAGCGAAA CCCCGTCGCA GCAGCGCTAC ATTTGCGAGG TGCTGGCGCC CTTCGCCTGG 
ACGTATGTGA CGGTGGCGCA GTCCCTGCAA ATACTGCACA AGAACTCCAT GCTGGAGTCT 
GAATTTATAA GCTTTGTGAT TAACGATCTG AGCAGCAAGG TCAAGCGCGG CAGCTGCATC 
TATGCCGAAA GCATATCCAC CGACTCCGTG CGCAACTGCC TGAAGCTGTT GGAGAAATGG 
TCTGTGATCG AGGTTTGCAA CCAGCAGGGC ATGCGCCTGA TCTCACTCAA CACGCTCTAC 
GAGATGTCTC GTGAGTCGCT CAACTCGATC GTGAAGAAGA TCGACGCAAT TGTACCAAAA 
TTCAACTCGG GCTACTTCAG TGTAACGCCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651597.1
CDS	691..3243
Translation	MLTTVLNFAQRVAEISLLGGIEIASVALIVYYIFAKTRAPVIIASLFPNLNLSYRTSYLSKINTIRSDLLVTLRSSNEPKTQNSVAHGIGTQNMLELTPHSGQKPFKKLFDWGVLFPYVAQVVRSEKFEYRQVTEIVHNDAVLKHAIKQAAEQTLREQRYAKNGHRLLTRSASGDQEAQEEEDEKRGISYQAILRKQEQRAISILKDMGSTLNNGLLAFTSWILYKLLPCFLSGVVTNTKQIEMLKTATERSPGTPLIFVPLHRSHLDYIMVTWILTNNDIRSPLVAAGNNLQIPVFGGLLRGLGAFFIKRKIDPVEGKKDVLYRAALHLYLTHALKQGHNVEFFIEGGRTRTGKPCMPKGGILSVIVNAFMDGSIPDALLVPVSVNYERLVDGNFVREQKGEKKIPESFGKAISGIWKALKSNYGLMRIDFNEPYSIRELVNSYNKIAREDGNIAKVYKPSARVLQHNQSTSSLYGTDVVCEEHRNLIESISRQVVFDCAAATSVMSTNALAFLLLTRFRNGAEEQILSEALDDLRNSLSGCKDIGFSGESSQIVAYACDLLGSGLVTRSRDENGRLVIKAVNSVESFIELAYYSNMLTPHFALSSILLTTFHSLLPETENKKEAAVSRKKLIDTALENCQIYRYEFILNKPTQVLENLLYQQLDDLLISGCVLTEKLHDDLPNGAEGRRLANVLAECLDEDGYEDVRDGEADEPKLLFASETPSQQRYICEVLAPFAWTYVTVAQSLQILHKNSMLESEFISFVINDLSSKVKRGSCIYAESISTDSVRNCLKLLEKWSVIEVCNQQGMRLISLNTLYEMSRESLNSIVKKIDAIVPKFNSGYFSVTP

Target ORF information:

RefSeq Version	NM_143340.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minotaur (mino), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143340.4

ORF Insert Sequence:

ATGTTGACGA CCGTCCTGAA CTTTGCCCAG CGCGTCGCCG AGATTTCTTT GCTCGGCGGC 
ATCGAGATTG CATCAGTTGC CCTTATTGTC TACTATATAT TCGCCAAGAC GAGGGCACCG 
GTAATCATCG CTAGCCTGTT TCCCAATCTG AATCTGAGCT ACCGGACGAG CTACCTCAGC 
AAGATCAACA CCATACGCAG CGATCTTCTG GTCACTTTGC GATCATCCAA TGAGCCCAAG 
ACGCAGAATT CGGTGGCTCA TGGCATTGGG ACGCAGAATA TGTTGGAGCT GACCCCGCAT 
TCGGGTCAGA AGCCTTTTAA GAAGCTATTC GACTGGGGCG TGCTCTTCCC ATATGTTGCC 
CAGGTGGTGC GCAGCGAGAA GTTCGAGTAC CGACAGGTCA CCGAGATCGT GCACAACGAT 
GCTGTACTGA AGCATGCAAT TAAACAGGCC GCCGAGCAGA CGCTAAGGGA GCAGAGATAT 
GCTAAAAATG GTCATCGTCT GCTCACGCGC AGTGCTAGTG GAGATCAGGA GGCGCAGGAG 
GAGGAGGACG AGAAGAGGGG TATATCCTAT CAGGCCATTC TGCGTAAGCA GGAGCAGCGC 
GCCATTAGCA TACTGAAGGA CATGGGCTCC ACTCTGAACA ATGGCCTTTT GGCCTTCACT 
TCCTGGATCC TGTACAAGTT GCTGCCCTGC TTCCTCTCCG GAGTCGTTAC GAACACGAAG 
CAGATCGAGA TGCTCAAAAC AGCCACAGAG CGTTCGCCGG GCACTCCGCT GATATTTGTT 
CCGCTGCATC GCAGCCACTT GGACTACATC ATGGTCACAT GGATACTCAC GAACAACGAT 
ATACGCAGTC CTCTGGTAGC CGCTGGCAAC AACCTGCAAA TTCCGGTCTT TGGTGGGCTC 
CTACGTGGTT TGGGTGCTTT CTTCATCAAA CGCAAGATTG ATCCTGTGGA GGGCAAGAAG 
GACGTGCTTT ATCGCGCTGC TCTACACCTG TATCTGACCC ACGCCTTGAA GCAGGGCCAC 
AACGTCGAGT TCTTCATCGA GGGCGGACGA ACGCGCACTG GCAAGCCGTG CATGCCCAAG 
GGCGGCATTC TTTCTGTGAT TGTGAACGCC TTCATGGACG GCAGCATACC GGACGCACTG 
CTGGTACCCG TTTCCGTAAA CTACGAGCGT CTCGTCGACG GAAACTTTGT GCGCGAACAA 
AAGGGCGAGA AGAAGATACC CGAATCATTC GGCAAGGCCA TTTCGGGCAT TTGGAAGGCC 
CTGAAATCAA ACTACGGCCT CATGCGCATC GACTTTAACG AGCCCTACTC CATACGTGAG 
CTGGTCAATT CGTACAACAA GATAGCCCGA GAAGATGGAA ACATTGCCAA GGTCTACAAG 
CCATCTGCCA GGGTCCTACA GCACAATCAG TCCACCTCCT CCTTGTACGG CACCGATGTT 
GTGTGCGAGG AGCACCGCAA TCTCATCGAA AGCATCTCGC GCCAGGTGGT CTTTGACTGC 
GCAGCAGCCA CCTCTGTGAT GTCCACCAAT GCGTTAGCCT TTTTGCTGCT CACCCGCTTT 
AGGAACGGTG CCGAGGAGCA GATTCTGTCG GAGGCTCTGG ACGACTTGCG AAACTCGTTG 
TCGGGCTGCA AGGACATTGG TTTTTCCGGG GAATCCTCGC AAATTGTGGC ATATGCCTGT 
GACCTACTCG GCTCAGGTCT TGTGACTCGC TCTCGGGATG AGAACGGGCG TTTGGTGATT 
AAGGCTGTGA ATTCAGTGGA AAGCTTTATT GAGCTGGCTT ACTATTCGAA CATGCTGACG 
CCGCACTTTG CCCTCAGCTC GATATTGTTG ACCACCTTCC ACTCACTGCT TCCGGAAACG 
GAGAATAAGA AAGAAGCCGC CGTGTCGCGC AAAAAGCTGA TAGACACCGC GCTGGAAAAC 
TGCCAGATCT ATCGCTACGA ATTTATTCTG AACAAACCGA CGCAGGTGCT GGAAAATTTG 
CTGTACCAGC AGTTGGACGA CCTCTTGATT AGTGGTTGCG TTTTGACCGA AAAGCTCCAT 
GACGATTTGC CGAATGGCGC TGAGGGCAGG CGCTTGGCCA ACGTCCTGGC AGAGTGCCTG 
GACGAGGACG GCTACGAAGA CGTGCGCGAT GGCGAGGCGG ATGAGCCCAA ACTGCTGTTC 
GCCAGCGAAA CCCCGTCGCA GCAGCGCTAC ATTTGCGAGG TGCTGGCGCC CTTCGCCTGG 
ACGTATGTGA CGGTGGCGCA GTCCCTGCAA ATACTGCACA AGAACTCCAT GCTGGAGTCT 
GAATTTATAA GCTTTGTGAT TAACGATCTG AGCAGCAAGG TCAAGCGCGG CAGCTGCATC 
TATGCCGAAA GCATATCCAC CGACTCCGTG CGCAACTGCC TGAAGCTGTT GGAGAAATGG 
TCTGTGATCG AGGTTTGCAA CCAGCAGGGC ATGCGCCTGA TCTCACTCAA CACGCTCTAC 
GAGATGTCTC GTGAGTCGCT CAACTCGATC GTGAAGAAGA TCGACGCAAT TGTACCAAAA 
TTCAACTCGG GCTACTTCAG TGTAACGCCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mino ( NM_170358.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733237.1 Drosophila melanogaster minotaur (mino), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06370
Clone ID Related Accession (Same CDS sequence)	NM_170358.2
Accession Version	NM_170358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	minotaur, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_170358.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCATGG AGGAGTTTCA CGCTCGCGGC TGGTTCGAGG AGCCCAAGAC GCAGAATTCG 
GTGGCTCATG GCATTGGGAC GCAGAATATG TTGGAGCTGA CCCCGCATTC GGGTCAGAAG 
CCTTTTAAGA AGCTATTCGA CTGGGGCGTG CTCTTCCCAT ATGTTGCCCA GGTGGTGCGC 
AGCGAGAAGT TCGAGTACCG ACAGGTCACC GAGATCGTGC ACAACGATGC TGTACTGAAG 
CATGCAATTA AACAGGCCGC CGAGCAGACG CTAAGGGAGC AGAGATATGC TAAAAATGGT 
CATCGTCTGC TCACGCGCAG TGCTAGTGGA GATCAGGAGG CGCAGGAGGA GGAGGACGAG 
AAGAGGGGTA TATCCTATCA GGCCATTCTG CGTAAGCAGG AGCAGCGCGC CATTAGCATA 
CTGAAGGACA TGGGCTCCAC TCTGAACAAT GGCCTTTTGG CCTTCACTTC CTGGATCCTG 
TACAAGTTGC TGCCCTGCTT CCTCTCCGGA GTCGTTACGA ACACGAAGCA GATCGAGATG 
CTCAAAACAG CCACAGAGCG TTCGCCGGGC ACTCCGCTGA TATTTGTTCC GCTGCATCGC 
AGCCACTTGG ACTACATCAT GGTCACATGG ATACTCACGA ACAACGATAT ACGCAGTCCT 
CTGGTAGCCG CTGGCAACAA CCTGCAAATT CCGGTCTTTG GTGGGCTCCT ACGTGGTTTG 
GGTGCTTTCT TCATCAAACG CAAGATTGAT CCTGTGGAGG GCAAGAAGGA CGTGCTTTAT 
CGCGCTGCTC TACACCTGTA TCTGACCCAC GCCTTGAAGC AGGGCCACAA CGTCGAGTTC 
TTCATCGAGG GCGGACGAAC GCGCACTGGC AAGCCGTGCA TGCCCAAGGG CGGCATTCTT 
TCTGTGATTG TGAACGCCTT CATGGACGGC AGCATACCGG ACGCACTGCT GGTACCCGTT 
TCCGTAAACT ACGAGCGTCT CGTCGACGGA AACTTTGTGC GCGAACAAAA GGGCGAGAAG 
AAGATACCCG AATCATTCGG CAAGGCCATT TCGGGCATTT GGAAGGCCCT GAAATCAAAC 
TACGGCCTCA TGCGCATCGA CTTTAACGAG CCCTACTCCA TACGTGAGCT GGTCAATTCG 
TACAACAAGA TAGCCCGAGA AGATGGAAAC ATTGCCAAGG TCTACAAGCC ATCTGCCAGG 
GTCCTACAGC ACAATCAGTC CACCTCCTCC TTGTACGGCA CCGATGTTGT GTGCGAGGAG 
CACCGCAATC TCATCGAAAG CATCTCGCGC CAGGTGGTCT TTGACTGCGC AGCAGCCACC 
TCTGTGATGT CCACCAATGC GTTAGCCTTT TTGCTGCTCA CCCGCTTTAG GAACGGTGCC 
GAGGAGCAGA TTCTGTCGGA GGCTCTGGAC GACTTGCGAA ACTCGTTGTC GGGCTGCAAG 
GACATTGGTT TTTCCGGGGA ATCCTCGCAA ATTGTGGCAT ATGCCTGTGA CCTACTCGGC 
TCAGGTCTTG TGACTCGCTC TCGGGATGAG AACGGGCGTT TGGTGATTAA GGCTGTGAAT 
TCAGTGGAAA GCTTTATTGA GCTGGCTTAC TATTCGAACA TGCTGACGCC GCACTTTGCC 
CTCAGCTCGA TATTGTTGAC CACCTTCCAC TCACTGCTTC CGGAAACGGA GAATAAGAAA 
GAAGCCGCCG TGTCGCGCAA AAAGCTGATA GACACCGCGC TGGAAAACTG CCAGATCTAT 
CGCTACGAAT TTATTCTGAA CAAACCGACG CAGGTGCTGG AAAATTTGCT GTACCAGCAG 
TTGGACGACC TCTTGATTAG TGGTTGCGTT TTGACCGAAA AGCTCCATGA CGATTTGCCG 
AATGGCGCTG AGGGCAGGCG CTTGGCCAAC GTCCTGGCAG AGTGCCTGGA CGAGGACGGC 
TACGAAGACG TGCGCGATGG CGAGGCGGAT GAGCCCAAAC TGCTGTTCGC CAGCGAAACC 
CCGTCGCAGC AGCGCTACAT TTGCGAGGTG CTGGCGCCCT TCGCCTGGAC GTATGTGACG 
GTGGCGCAGT CCCTGCAAAT ACTGCACAAG AACTCCATGC TGGAGTCTGA ATTTATAAGC 
TTTGTGATTA ACGATCTGAG CAGCAAGGTC AAGCGCGGCA GCTGCATCTA TGCCGAAAGC 
ATATCCACCG ACTCCGTGCG CAACTGCCTG AAGCTGTTGG AGAAATGGTC TGTGATCGAG 
GTTTGCAACC AGCAGGGCAT GCGCCTGATC TCACTCAACA CGCTCTACGA GATGTCTCGT 
GAGTCGCTCA ACTCGATCGT GAAGAAGATC GACGCAATTG TACCAAAATT CAACTCGGGC 
TACTTCAGTG TAACGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733237.1
CDS	240..2600
Translation	MPMEEFHARGWFEEPKTQNSVAHGIGTQNMLELTPHSGQKPFKKLFDWGVLFPYVAQVVRSEKFEYRQVTEIVHNDAVLKHAIKQAAEQTLREQRYAKNGHRLLTRSASGDQEAQEEEDEKRGISYQAILRKQEQRAISILKDMGSTLNNGLLAFTSWILYKLLPCFLSGVVTNTKQIEMLKTATERSPGTPLIFVPLHRSHLDYIMVTWILTNNDIRSPLVAAGNNLQIPVFGGLLRGLGAFFIKRKIDPVEGKKDVLYRAALHLYLTHALKQGHNVEFFIEGGRTRTGKPCMPKGGILSVIVNAFMDGSIPDALLVPVSVNYERLVDGNFVREQKGEKKIPESFGKAISGIWKALKSNYGLMRIDFNEPYSIRELVNSYNKIAREDGNIAKVYKPSARVLQHNQSTSSLYGTDVVCEEHRNLIESISRQVVFDCAAATSVMSTNALAFLLLTRFRNGAEEQILSEALDDLRNSLSGCKDIGFSGESSQIVAYACDLLGSGLVTRSRDENGRLVIKAVNSVESFIELAYYSNMLTPHFALSSILLTTFHSLLPETENKKEAAVSRKKLIDTALENCQIYRYEFILNKPTQVLENLLYQQLDDLLISGCVLTEKLHDDLPNGAEGRRLANVLAECLDEDGYEDVRDGEADEPKLLFASETPSQQRYICEVLAPFAWTYVTVAQSLQILHKNSMLESEFISFVINDLSSKVKRGSCIYAESISTDSVRNCLKLLEKWSVIEVCNQQGMRLISLNTLYEMSRESLNSIVKKIDAIVPKFNSGYFSVTP

Target ORF information:

RefSeq Version	NM_170358.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minotaur (mino), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170358.2

ORF Insert Sequence:

ATGCCCATGG AGGAGTTTCA CGCTCGCGGC TGGTTCGAGG AGCCCAAGAC GCAGAATTCG 
GTGGCTCATG GCATTGGGAC GCAGAATATG TTGGAGCTGA CCCCGCATTC GGGTCAGAAG 
CCTTTTAAGA AGCTATTCGA CTGGGGCGTG CTCTTCCCAT ATGTTGCCCA GGTGGTGCGC 
AGCGAGAAGT TCGAGTACCG ACAGGTCACC GAGATCGTGC ACAACGATGC TGTACTGAAG 
CATGCAATTA AACAGGCCGC CGAGCAGACG CTAAGGGAGC AGAGATATGC TAAAAATGGT 
CATCGTCTGC TCACGCGCAG TGCTAGTGGA GATCAGGAGG CGCAGGAGGA GGAGGACGAG 
AAGAGGGGTA TATCCTATCA GGCCATTCTG CGTAAGCAGG AGCAGCGCGC CATTAGCATA 
CTGAAGGACA TGGGCTCCAC TCTGAACAAT GGCCTTTTGG CCTTCACTTC CTGGATCCTG 
TACAAGTTGC TGCCCTGCTT CCTCTCCGGA GTCGTTACGA ACACGAAGCA GATCGAGATG 
CTCAAAACAG CCACAGAGCG TTCGCCGGGC ACTCCGCTGA TATTTGTTCC GCTGCATCGC 
AGCCACTTGG ACTACATCAT GGTCACATGG ATACTCACGA ACAACGATAT ACGCAGTCCT 
CTGGTAGCCG CTGGCAACAA CCTGCAAATT CCGGTCTTTG GTGGGCTCCT ACGTGGTTTG 
GGTGCTTTCT TCATCAAACG CAAGATTGAT CCTGTGGAGG GCAAGAAGGA CGTGCTTTAT 
CGCGCTGCTC TACACCTGTA TCTGACCCAC GCCTTGAAGC AGGGCCACAA CGTCGAGTTC 
TTCATCGAGG GCGGACGAAC GCGCACTGGC AAGCCGTGCA TGCCCAAGGG CGGCATTCTT 
TCTGTGATTG TGAACGCCTT CATGGACGGC AGCATACCGG ACGCACTGCT GGTACCCGTT 
TCCGTAAACT ACGAGCGTCT CGTCGACGGA AACTTTGTGC GCGAACAAAA GGGCGAGAAG 
AAGATACCCG AATCATTCGG CAAGGCCATT TCGGGCATTT GGAAGGCCCT GAAATCAAAC 
TACGGCCTCA TGCGCATCGA CTTTAACGAG CCCTACTCCA TACGTGAGCT GGTCAATTCG 
TACAACAAGA TAGCCCGAGA AGATGGAAAC ATTGCCAAGG TCTACAAGCC ATCTGCCAGG 
GTCCTACAGC ACAATCAGTC CACCTCCTCC TTGTACGGCA CCGATGTTGT GTGCGAGGAG 
CACCGCAATC TCATCGAAAG CATCTCGCGC CAGGTGGTCT TTGACTGCGC AGCAGCCACC 
TCTGTGATGT CCACCAATGC GTTAGCCTTT TTGCTGCTCA CCCGCTTTAG GAACGGTGCC 
GAGGAGCAGA TTCTGTCGGA GGCTCTGGAC GACTTGCGAA ACTCGTTGTC GGGCTGCAAG 
GACATTGGTT TTTCCGGGGA ATCCTCGCAA ATTGTGGCAT ATGCCTGTGA CCTACTCGGC 
TCAGGTCTTG TGACTCGCTC TCGGGATGAG AACGGGCGTT TGGTGATTAA GGCTGTGAAT 
TCAGTGGAAA GCTTTATTGA GCTGGCTTAC TATTCGAACA TGCTGACGCC GCACTTTGCC 
CTCAGCTCGA TATTGTTGAC CACCTTCCAC TCACTGCTTC CGGAAACGGA GAATAAGAAA 
GAAGCCGCCG TGTCGCGCAA AAAGCTGATA GACACCGCGC TGGAAAACTG CCAGATCTAT 
CGCTACGAAT TTATTCTGAA CAAACCGACG CAGGTGCTGG AAAATTTGCT GTACCAGCAG 
TTGGACGACC TCTTGATTAG TGGTTGCGTT TTGACCGAAA AGCTCCATGA CGATTTGCCG 
AATGGCGCTG AGGGCAGGCG CTTGGCCAAC GTCCTGGCAG AGTGCCTGGA CGAGGACGGC 
TACGAAGACG TGCGCGATGG CGAGGCGGAT GAGCCCAAAC TGCTGTTCGC CAGCGAAACC 
CCGTCGCAGC AGCGCTACAT TTGCGAGGTG CTGGCGCCCT TCGCCTGGAC GTATGTGACG 
GTGGCGCAGT CCCTGCAAAT ACTGCACAAG AACTCCATGC TGGAGTCTGA ATTTATAAGC 
TTTGTGATTA ACGATCTGAG CAGCAAGGTC AAGCGCGGCA GCTGCATCTA TGCCGAAAGC 
ATATCCACCG ACTCCGTGCG CAACTGCCTG AAGCTGTTGG AGAAATGGTC TGTGATCGAG 
GTTTGCAACC AGCAGGGCAT GCGCCTGATC TCACTCAACA CGCTCTACGA GATGTCTCGT 
GAGTCGCTCA ACTCGATCGT GAAGAAGATC GACGCAATTG TACCAAAATT CAACTCGGGC 
TACTTCAGTG TAACGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mino ( NM_170359.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733238.1 Drosophila melanogaster minotaur (mino), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06371
Clone ID Related Accession (Same CDS sequence)	NM_170359.3
Accession Version	NM_170359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	minotaur, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170359.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGAGC TGACCCCGCA TTCGGGTCAG AAGCCTTTTA AGAAGCTATT CGACTGGGGC 
GTGCTCTTCC CATATGTTGC CCAGGTGGTG CGCAGCGAGA AGTTCGAGTA CCGACAGGTC 
ACCGAGATCG TGCACAACGA TGCTGTACTG AAGCATGCAA TTAAACAGGC CGCCGAGCAG 
ACGCTAAGGG AGCAGAGATA TGCTAAAAAT GGTCATCGTC TGCTCACGCG CAGTGCTAGT 
GGAGATCAGG AGGCGCAGGA GGAGGAGGAC GAGAAGAGGG GTATATCCTA TCAGGCCATT 
CTGCGTAAGC AGGAGCAGCG CGCCATTAGC ATACTGAAGG ACATGGGCTC CACTCTGAAC 
AATGGCCTTT TGGCCTTCAC TTCCTGGATC CTGTACAAGT TGCTGCCCTG CTTCCTCTCC 
GGAGTCGTTA CGAACACGAA GCAGATCGAG ATGCTCAAAA CAGCCACAGA GCGTTCGCCG 
GGCACTCCGC TGATATTTGT TCCGCTGCAT CGCAGCCACT TGGACTACAT CATGGTCACA 
TGGATACTCA CGAACAACGA TATACGCAGT CCTCTGGTAG CCGCTGGCAA CAACCTGCAA 
ATTCCGGTCT TTGGTGGGCT CCTACGTGGT TTGGGTGCTT TCTTCATCAA ACGCAAGATT 
GATCCTGTGG AGGGCAAGAA GGACGTGCTT TATCGCGCTG CTCTACACCT GTATCTGACC 
CACGCCTTGA AGCAGGGCCA CAACGTCGAG TTCTTCATCG AGGGCGGACG AACGCGCACT 
GGCAAGCCGT GCATGCCCAA GGGCGGCATT CTTTCTGTGA TTGTGAACGC CTTCATGGAC 
GGCAGCATAC CGGACGCACT GCTGGTACCC GTTTCCGTAA ACTACGAGCG TCTCGTCGAC 
GGAAACTTTG TGCGCGAACA AAAGGGCGAG AAGAAGATAC CCGAATCATT CGGCAAGGCC 
ATTTCGGGCA TTTGGAAGGC CCTGAAATCA AACTACGGCC TCATGCGCAT CGACTTTAAC 
GAGCCCTACT CCATACGTGA GCTGGTCAAT TCGTACAACA AGATAGCCCG AGAAGATGGA 
AACATTGCCA AGGTCTACAA GCCATCTGCC AGGGTCCTAC AGCACAATCA GTCCACCTCC 
TCCTTGTACG GCACCGATGT TGTGTGCGAG GAGCACCGCA ATCTCATCGA AAGCATCTCG 
CGCCAGGTGG TCTTTGACTG CGCAGCAGCC ACCTCTGTGA TGTCCACCAA TGCGTTAGCC 
TTTTTGCTGC TCACCCGCTT TAGGAACGGT GCCGAGGAGC AGATTCTGTC GGAGGCTCTG 
GACGACTTGC GAAACTCGTT GTCGGGCTGC AAGGACATTG GTTTTTCCGG GGAATCCTCG 
CAAATTGTGG CATATGCCTG TGACCTACTC GGCTCAGGTC TTGTGACTCG CTCTCGGGAT 
GAGAACGGGC GTTTGGTGAT TAAGGCTGTG AATTCAGTGG AAAGCTTTAT TGAGCTGGCT 
TACTATTCGA ACATGCTGAC GCCGCACTTT GCCCTCAGCT CGATATTGTT GACCACCTTC 
CACTCACTGC TTCCGGAAAC GGAGAATAAG AAAGAAGCCG CCGTGTCGCG CAAAAAGCTG 
ATAGACACCG CGCTGGAAAA CTGCCAGATC TATCGCTACG AATTTATTCT GAACAAACCG 
ACGCAGGTGC TGGAAAATTT GCTGTACCAG CAGTTGGACG ACCTCTTGAT TAGTGGTTGC 
GTTTTGACCG AAAAGCTCCA TGACGATTTG CCGAATGGCG CTGAGGGCAG GCGCTTGGCC 
AACGTCCTGG CAGAGTGCCT GGACGAGGAC GGCTACGAAG ACGTGCGCGA TGGCGAGGCG 
GATGAGCCCA AACTGCTGTT CGCCAGCGAA ACCCCGTCGC AGCAGCGCTA CATTTGCGAG 
GTGCTGGCGC CCTTCGCCTG GACGTATGTG ACGGTGGCGC AGTCCCTGCA AATACTGCAC 
AAGAACTCCA TGCTGGAGTC TGAATTTATA AGCTTTGTGA TTAACGATCT GAGCAGCAAG 
GTCAAGCGCG GCAGCTGCAT CTATGCCGAA AGCATATCCA CCGACTCCGT GCGCAACTGC 
CTGAAGCTGT TGGAGAAATG GTCTGTGATC GAGGTTTGCA ACCAGCAGGG CATGCGCCTG 
ATCTCACTCA ACACGCTCTA CGAGATGTCT CGTGAGTCGC TCAACTCGAT CGTGAAGAAG 
ATCGACGCAA TTGTACCAAA ATTCAACTCG GGCTACTTCA GTGTAACGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733238.1
CDS	216..2489
Translation	MLELTPHSGQKPFKKLFDWGVLFPYVAQVVRSEKFEYRQVTEIVHNDAVLKHAIKQAAEQTLREQRYAKNGHRLLTRSASGDQEAQEEEDEKRGISYQAILRKQEQRAISILKDMGSTLNNGLLAFTSWILYKLLPCFLSGVVTNTKQIEMLKTATERSPGTPLIFVPLHRSHLDYIMVTWILTNNDIRSPLVAAGNNLQIPVFGGLLRGLGAFFIKRKIDPVEGKKDVLYRAALHLYLTHALKQGHNVEFFIEGGRTRTGKPCMPKGGILSVIVNAFMDGSIPDALLVPVSVNYERLVDGNFVREQKGEKKIPESFGKAISGIWKALKSNYGLMRIDFNEPYSIRELVNSYNKIAREDGNIAKVYKPSARVLQHNQSTSSLYGTDVVCEEHRNLIESISRQVVFDCAAATSVMSTNALAFLLLTRFRNGAEEQILSEALDDLRNSLSGCKDIGFSGESSQIVAYACDLLGSGLVTRSRDENGRLVIKAVNSVESFIELAYYSNMLTPHFALSSILLTTFHSLLPETENKKEAAVSRKKLIDTALENCQIYRYEFILNKPTQVLENLLYQQLDDLLISGCVLTEKLHDDLPNGAEGRRLANVLAECLDEDGYEDVRDGEADEPKLLFASETPSQQRYICEVLAPFAWTYVTVAQSLQILHKNSMLESEFISFVINDLSSKVKRGSCIYAESISTDSVRNCLKLLEKWSVIEVCNQQGMRLISLNTLYEMSRESLNSIVKKIDAIVPKFNSGYFSVTP

Target ORF information:

RefSeq Version	NM_170359.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minotaur (mino), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170359.3

ORF Insert Sequence:

ATGTTGGAGC TGACCCCGCA TTCGGGTCAG AAGCCTTTTA AGAAGCTATT CGACTGGGGC 
GTGCTCTTCC CATATGTTGC CCAGGTGGTG CGCAGCGAGA AGTTCGAGTA CCGACAGGTC 
ACCGAGATCG TGCACAACGA TGCTGTACTG AAGCATGCAA TTAAACAGGC CGCCGAGCAG 
ACGCTAAGGG AGCAGAGATA TGCTAAAAAT GGTCATCGTC TGCTCACGCG CAGTGCTAGT 
GGAGATCAGG AGGCGCAGGA GGAGGAGGAC GAGAAGAGGG GTATATCCTA TCAGGCCATT 
CTGCGTAAGC AGGAGCAGCG CGCCATTAGC ATACTGAAGG ACATGGGCTC CACTCTGAAC 
AATGGCCTTT TGGCCTTCAC TTCCTGGATC CTGTACAAGT TGCTGCCCTG CTTCCTCTCC 
GGAGTCGTTA CGAACACGAA GCAGATCGAG ATGCTCAAAA CAGCCACAGA GCGTTCGCCG 
GGCACTCCGC TGATATTTGT TCCGCTGCAT CGCAGCCACT TGGACTACAT CATGGTCACA 
TGGATACTCA CGAACAACGA TATACGCAGT CCTCTGGTAG CCGCTGGCAA CAACCTGCAA 
ATTCCGGTCT TTGGTGGGCT CCTACGTGGT TTGGGTGCTT TCTTCATCAA ACGCAAGATT 
GATCCTGTGG AGGGCAAGAA GGACGTGCTT TATCGCGCTG CTCTACACCT GTATCTGACC 
CACGCCTTGA AGCAGGGCCA CAACGTCGAG TTCTTCATCG AGGGCGGACG AACGCGCACT 
GGCAAGCCGT GCATGCCCAA GGGCGGCATT CTTTCTGTGA TTGTGAACGC CTTCATGGAC 
GGCAGCATAC CGGACGCACT GCTGGTACCC GTTTCCGTAA ACTACGAGCG TCTCGTCGAC 
GGAAACTTTG TGCGCGAACA AAAGGGCGAG AAGAAGATAC CCGAATCATT CGGCAAGGCC 
ATTTCGGGCA TTTGGAAGGC CCTGAAATCA AACTACGGCC TCATGCGCAT CGACTTTAAC 
GAGCCCTACT CCATACGTGA GCTGGTCAAT TCGTACAACA AGATAGCCCG AGAAGATGGA 
AACATTGCCA AGGTCTACAA GCCATCTGCC AGGGTCCTAC AGCACAATCA GTCCACCTCC 
TCCTTGTACG GCACCGATGT TGTGTGCGAG GAGCACCGCA ATCTCATCGA AAGCATCTCG 
CGCCAGGTGG TCTTTGACTG CGCAGCAGCC ACCTCTGTGA TGTCCACCAA TGCGTTAGCC 
TTTTTGCTGC TCACCCGCTT TAGGAACGGT GCCGAGGAGC AGATTCTGTC GGAGGCTCTG 
GACGACTTGC GAAACTCGTT GTCGGGCTGC AAGGACATTG GTTTTTCCGG GGAATCCTCG 
CAAATTGTGG CATATGCCTG TGACCTACTC GGCTCAGGTC TTGTGACTCG CTCTCGGGAT 
GAGAACGGGC GTTTGGTGAT TAAGGCTGTG AATTCAGTGG AAAGCTTTAT TGAGCTGGCT 
TACTATTCGA ACATGCTGAC GCCGCACTTT GCCCTCAGCT CGATATTGTT GACCACCTTC 
CACTCACTGC TTCCGGAAAC GGAGAATAAG AAAGAAGCCG CCGTGTCGCG CAAAAAGCTG 
ATAGACACCG CGCTGGAAAA CTGCCAGATC TATCGCTACG AATTTATTCT GAACAAACCG 
ACGCAGGTGC TGGAAAATTT GCTGTACCAG CAGTTGGACG ACCTCTTGAT TAGTGGTTGC 
GTTTTGACCG AAAAGCTCCA TGACGATTTG CCGAATGGCG CTGAGGGCAG GCGCTTGGCC 
AACGTCCTGG CAGAGTGCCT GGACGAGGAC GGCTACGAAG ACGTGCGCGA TGGCGAGGCG 
GATGAGCCCA AACTGCTGTT CGCCAGCGAA ACCCCGTCGC AGCAGCGCTA CATTTGCGAG 
GTGCTGGCGC CCTTCGCCTG GACGTATGTG ACGGTGGCGC AGTCCCTGCA AATACTGCAC 
AAGAACTCCA TGCTGGAGTC TGAATTTATA AGCTTTGTGA TTAACGATCT GAGCAGCAAG 
GTCAAGCGCG GCAGCTGCAT CTATGCCGAA AGCATATCCA CCGACTCCGT GCGCAACTGC 
CTGAAGCTGT TGGAGAAATG GTCTGTGATC GAGGTTTGCA ACCAGCAGGG CATGCGCCTG 
ATCTCACTCA ACACGCTCTA CGAGATGTCT CGTGAGTCGC TCAACTCGAT CGTGAAGAAG 
ATCGACGCAA TTGTACCAAA ATTCAACTCG GGCTACTTCA GTGTAACGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mino cDNA ORF clone, Drosophila melanogaster(fruit fly)

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