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Ank cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol Ank
Entrez Gene ID 43770
Full Name Ankyrin
Synonyms ANK,CG1651,CG32008,DAnk1,Dank1,Dmel\CG1651,ank,dank1
General protein information
Preferred Names

Ankyrin

Names

CG1651 gene product from transcript CG1651-RE
Ank-PB
Ank-PC
Ank-PD
Ank-PE
CG1651-PB
CG1651-PC
CG1651-PD
CG1651-PE
ankyrin
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

4

102A3-102A3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_175926.3 NP_787122.1 ankyrin, isoform B
NM_001169348.2 NP_001162819.1 ankyrin, isoform E
NM_175928.3 NP_787124.1 ankyrin, isoform D
NM_175927.3 NP_787123.1 ankyrin, isoform C

Pathways from BioSystems


Homologies


Gallus gallus (chicken) ANK3 NP_001264026.1
Danio rerio (zebrafish) ank3b XP_005156095.1
Drosophila melanogaster (fruit fly) Ank NP_001162819.1
Xenopus tropicalis (tropical clawed frog) ank3 XP_004915849.1
ANK3 XP_002808549.1
Homo sapiens (human) ANK3 NP_066267.2
Pan troglodytes (chimpanzee) ANK3 XP_003312618.1
Mus musculus (house mouse) Ank3 NP_666117.2
Rattus norvegicus (Norway rat) Ank3 NP_001029156.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009937 XP_319063.4
Canis lupus familiaris (dog) ANK3 XP_536358.3

Gene Ontology


Bibliography

Related articles in PubMed

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.
Hoskins RA, Smith CD, Carlson JW, Carvalho AB, Halpern A, Kaminker JS, Kennedy C, Mungall CJ, Sullivan BA, Sutton GG, Yasuhara JC, Wakimoto BT, Myers EW, Celniker SE, Rubin GM, Karpen GH
Genome biology3(12)RESEARCH0085(2002)

The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective.
Kaminker JS, Bergman CM, Kronmiller B, Carlson J, Svirskas R, Patel S, Frise E, Wheeler DA, Lewis SE, Rubin GM, Ashburner M, Celniker SE
Genome biology3(12)RESEARCH0084(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this study, use of osmolyte cosolvents demonstrates that hydrogen-bonding contributions can be separated from hydrophobic interactions in the denatured state of a truncated notch ankyrin protein domain.
Title: Hydrogen bonding progressively strengthens upon transfer of the protein urea-denatured state to water and protecting osmolytes.

Molecular mechanisms that enhance synapse stability despite persistent disruption of the spectrin/Ank/microtubule cytoskeleton are reported.
Title: Molecular mechanisms that enhance synapse stability despite persistent disruption of the spectrin/ankyrin/microtubule cytoskeleton.

the Spectrin network is playing a role independently of Ankyrin in the establishment and/or maintenance of specialized membrane domains containing guidance molecules that ensure the fidelity of axon repulsion at the midline
Title: beta-Spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS.

The following Ank gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ank cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa00135 NM_001169348.2
Latest version!		 Drosophila melanogaster ankyrin, transcript variant E (Ank), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $881.30
$1259.00
OFa00135 NM_175927.3
Latest version!		 Drosophila melanogaster ankyrin, transcript variant C (Ank), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $881.30
$1259.00
OFa00135 NM_175926.3
Latest version!		 Drosophila melanogaster ankyrin, transcript variant B (Ank), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $881.30
$1259.00
OFa00135 NM_175928.3
Latest version!		 Drosophila melanogaster ankyrin, transcript variant D (Ank), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa00135
Clone ID Related Accession (Same CDS sequence) NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version NM_175926.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-03-14
Organism Drosophila melanogaster(fruit fly)
Product ankyrin, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_175926.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787122.1
CDS27..4676
Misc Feature(1)225..602
Misc Feature(2)240..335
Misc Feature(3)255..527
Misc Feature(4)339..434
Misc Feature(5)438..533
Misc Feature(6)636..986
Misc Feature(7)636..719
Misc Feature(8)639..911
Misc Feature(9)order(642..647,654..662,666..671,681..683,690..692,717..719,723..725,729..731,741..746,753..761,765..770,780..782,789..791,816..818,822..824,828..830,840..845,852..860,864..869,879..881,888..890)
Misc Feature(10)723..818
Misc Feature(11)822..911
Misc Feature(12)906..1283
Misc Feature(13)order(921..923,927..929,939..944,951..959,963..968,978..980,987..989,1014..1016,1020..1022,1026..1028,1038..1043,1050..1058,1062..1067,1077..1079,1086..1088,1113..1115,1119..1121,1125..1127,1137..1142,1149..1157,1161..1166,1176..1178,1185..1187)
Misc Feature(14)921..1016
Misc Feature(15)924..1085
Misc Feature(16)1020..1115
Misc Feature(17)1035..1310
Misc Feature(18)1104..1481
Misc Feature(19)1119..1211
Misc Feature(20)1218..1307
Misc Feature(21)1317..1412
Misc Feature(22)1332..1604
Misc Feature(23)1416..1508
Misc Feature(24)1497..1874
Misc Feature(25)order(1512..1514,1518..1520,1530..1535,1542..1550,1554..1559,1569..1571,1578..1580,1605..1607,1611..1613,1617..1619,1629..1634,1641..1649,1653..1658,1668..1670,1677..1679,1704..1706,1710..1712,1716..1718,1728..1733,1740..1748,1752..1757,1767..1769,1776..1778)
Misc Feature(26)1512..1607
Misc Feature(27)1527..1802
Misc Feature(28)1611..1706
Misc Feature(29)1695..2072
Misc Feature(30)1710..1796
Misc Feature(31)1770..1934
Misc Feature(32)1809..1901
Misc Feature(33)1908..1997
Misc Feature(34)1923..2195
Misc Feature(35)order(2007..2009,2013..2015,2025..2030,2037..2045,2049..2054,2064..2066,2073..2075,2097..2099,2103..2105,2109..2111,2121..2126,2133..2141,2145..2150,2160..2162,2169..2171,2196..2198,2202..2204,2208..2210,2220..2225,2232..2240,2244..2249,2259..2261,2268..2270)
Misc Feature(36)2007..2099
Misc Feature(37)2100..2462
Misc Feature(38)2103..2198
Misc Feature(39)2118..2390
Misc Feature(40)2202..2291
Misc Feature(41)2301..2390
Misc Feature(42)2814..3128
Misc Feature(43)4341..4589
Translation

Target ORF information:

RefSeq Version NM_175926.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster ankyrin, transcript variant B (Ank), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175926.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa00135
Clone ID Related Accession (Same CDS sequence) NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version NM_001169348.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-03-14
Organism Drosophila melanogaster(fruit fly)
Product ankyrin, isoform E
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001169348.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001162819.1
CDS69..4718
Misc Feature(1)267..644
Misc Feature(2)282..377
Misc Feature(3)297..569
Misc Feature(4)381..476
Misc Feature(5)480..575
Misc Feature(6)678..1028
Misc Feature(7)678..761
Misc Feature(8)681..953
Misc Feature(9)order(684..689,696..704,708..713,723..725,732..734,759..761,765..767,771..773,783..788,795..803,807..812,822..824,831..833,858..860,864..866,870..872,882..887,894..902,906..911,921..923,930..932)
Misc Feature(10)765..860
Misc Feature(11)864..953
Misc Feature(12)948..1325
Misc Feature(13)order(963..965,969..971,981..986,993..1001,1005..1010,1020..1022,1029..1031,1056..1058,1062..1064,1068..1070,1080..1085,1092..1100,1104..1109,1119..1121,1128..1130,1155..1157,1161..1163,1167..1169,1179..1184,1191..1199,1203..1208,1218..1220,1227..1229)
Misc Feature(14)963..1058
Misc Feature(15)966..1127
Misc Feature(16)1062..1157
Misc Feature(17)1077..1352
Misc Feature(18)1146..1523
Misc Feature(19)1161..1253
Misc Feature(20)1260..1349
Misc Feature(21)1359..1454
Misc Feature(22)1374..1646
Misc Feature(23)1458..1550
Misc Feature(24)1539..1916
Misc Feature(25)order(1554..1556,1560..1562,1572..1577,1584..1592,1596..1601,1611..1613,1620..1622,1647..1649,1653..1655,1659..1661,1671..1676,1683..1691,1695..1700,1710..1712,1719..1721,1746..1748,1752..1754,1758..1760,1770..1775,1782..1790,1794..1799,1809..1811,1818..1820)
Misc Feature(26)1554..1649
Misc Feature(27)1569..1844
Misc Feature(28)1653..1748
Misc Feature(29)1737..2114
Misc Feature(30)1752..1838
Misc Feature(31)1812..1976
Misc Feature(32)1851..1943
Misc Feature(33)1950..2039
Misc Feature(34)1965..2237
Misc Feature(35)order(2049..2051,2055..2057,2067..2072,2079..2087,2091..2096,2106..2108,2115..2117,2139..2141,2145..2147,2151..2153,2163..2168,2175..2183,2187..2192,2202..2204,2211..2213,2238..2240,2244..2246,2250..2252,2262..2267,2274..2282,2286..2291,2301..2303,2310..2312)
Misc Feature(36)2049..2141
Misc Feature(37)2142..2504
Misc Feature(38)2145..2240
Misc Feature(39)2160..2432
Misc Feature(40)2244..2333
Misc Feature(41)2343..2432
Misc Feature(42)2856..3170
Misc Feature(43)4383..4631
Translation

Target ORF information:

RefSeq Version NM_001169348.2
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster ankyrin, transcript variant E (Ank), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001169348.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa00135
Clone ID Related Accession (Same CDS sequence) NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version NM_175928.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-03-14
Organism Drosophila melanogaster(fruit fly)
Product ankyrin, isoform D
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_175928.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787124.1
CDS217..4866
Misc Feature(1)415..792
Misc Feature(2)430..525
Misc Feature(3)445..717
Misc Feature(4)529..624
Misc Feature(5)628..723
Misc Feature(6)826..1176
Misc Feature(7)826..909
Misc Feature(8)829..1101
Misc Feature(9)order(832..837,844..852,856..861,871..873,880..882,907..909,913..915,919..921,931..936,943..951,955..960,970..972,979..981,1006..1008,1012..1014,1018..1020,1030..1035,1042..1050,1054..1059,1069..1071,1078..1080)
Misc Feature(10)913..1008
Misc Feature(11)1012..1101
Misc Feature(12)1096..1473
Misc Feature(13)order(1111..1113,1117..1119,1129..1134,1141..1149,1153..1158,1168..1170,1177..1179,1204..1206,1210..1212,1216..1218,1228..1233,1240..1248,1252..1257,1267..1269,1276..1278,1303..1305,1309..1311,1315..1317,1327..1332,1339..1347,1351..1356,1366..1368,1375..1377)
Misc Feature(14)1111..1206
Misc Feature(15)1114..1275
Misc Feature(16)1210..1305
Misc Feature(17)1225..1500
Misc Feature(18)1294..1671
Misc Feature(19)1309..1401
Misc Feature(20)1408..1497
Misc Feature(21)1507..1602
Misc Feature(22)1522..1794
Misc Feature(23)1606..1698
Misc Feature(24)1687..2064
Misc Feature(25)order(1702..1704,1708..1710,1720..1725,1732..1740,1744..1749,1759..1761,1768..1770,1795..1797,1801..1803,1807..1809,1819..1824,1831..1839,1843..1848,1858..1860,1867..1869,1894..1896,1900..1902,1906..1908,1918..1923,1930..1938,1942..1947,1957..1959,1966..1968)
Misc Feature(26)1702..1797
Misc Feature(27)1717..1992
Misc Feature(28)1801..1896
Misc Feature(29)1885..2262
Misc Feature(30)1900..1986
Misc Feature(31)1960..2124
Misc Feature(32)1999..2091
Misc Feature(33)2098..2187
Misc Feature(34)2113..2385
Misc Feature(35)order(2197..2199,2203..2205,2215..2220,2227..2235,2239..2244,2254..2256,2263..2265,2287..2289,2293..2295,2299..2301,2311..2316,2323..2331,2335..2340,2350..2352,2359..2361,2386..2388,2392..2394,2398..2400,2410..2415,2422..2430,2434..2439,2449..2451,2458..2460)
Misc Feature(36)2197..2289
Misc Feature(37)2290..2652
Misc Feature(38)2293..2388
Misc Feature(39)2308..2580
Misc Feature(40)2392..2481
Misc Feature(41)2491..2580
Misc Feature(42)3004..3318
Misc Feature(43)4531..4779
Translation

Target ORF information:

RefSeq Version NM_175928.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster ankyrin, transcript variant D (Ank), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175928.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa00135
Clone ID Related Accession (Same CDS sequence) NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version NM_175927.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-03-14
Organism Drosophila melanogaster(fruit fly)
Product ankyrin, isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_175927.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.13 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787123.1
CDS102..4751
Misc Feature(1)300..677
Misc Feature(2)315..410
Misc Feature(3)330..602
Misc Feature(4)414..509
Misc Feature(5)513..608
Misc Feature(6)711..1061
Misc Feature(7)711..794
Misc Feature(8)714..986
Misc Feature(9)order(717..722,729..737,741..746,756..758,765..767,792..794,798..800,804..806,816..821,828..836,840..845,855..857,864..866,891..893,897..899,903..905,915..920,927..935,939..944,954..956,963..965)
Misc Feature(10)798..893
Misc Feature(11)897..986
Misc Feature(12)981..1358
Misc Feature(13)order(996..998,1002..1004,1014..1019,1026..1034,1038..1043,1053..1055,1062..1064,1089..1091,1095..1097,1101..1103,1113..1118,1125..1133,1137..1142,1152..1154,1161..1163,1188..1190,1194..1196,1200..1202,1212..1217,1224..1232,1236..1241,1251..1253,1260..1262)
Misc Feature(14)996..1091
Misc Feature(15)999..1160
Misc Feature(16)1095..1190
Misc Feature(17)1110..1385
Misc Feature(18)1179..1556
Misc Feature(19)1194..1286
Misc Feature(20)1293..1382
Misc Feature(21)1392..1487
Misc Feature(22)1407..1679
Misc Feature(23)1491..1583
Misc Feature(24)1572..1949
Misc Feature(25)order(1587..1589,1593..1595,1605..1610,1617..1625,1629..1634,1644..1646,1653..1655,1680..1682,1686..1688,1692..1694,1704..1709,1716..1724,1728..1733,1743..1745,1752..1754,1779..1781,1785..1787,1791..1793,1803..1808,1815..1823,1827..1832,1842..1844,1851..1853)
Misc Feature(26)1587..1682
Misc Feature(27)1602..1877
Misc Feature(28)1686..1781
Misc Feature(29)1770..2147
Misc Feature(30)1785..1871
Misc Feature(31)1845..2009
Misc Feature(32)1884..1976
Misc Feature(33)1983..2072
Misc Feature(34)1998..2270
Misc Feature(35)order(2082..2084,2088..2090,2100..2105,2112..2120,2124..2129,2139..2141,2148..2150,2172..2174,2178..2180,2184..2186,2196..2201,2208..2216,2220..2225,2235..2237,2244..2246,2271..2273,2277..2279,2283..2285,2295..2300,2307..2315,2319..2324,2334..2336,2343..2345)
Misc Feature(36)2082..2174
Misc Feature(37)2175..2537
Misc Feature(38)2178..2273
Misc Feature(39)2193..2465
Misc Feature(40)2277..2366
Misc Feature(41)2376..2465
Misc Feature(42)2889..3203
Misc Feature(43)4416..4664
Translation

Target ORF information:

RefSeq Version NM_175927.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster ankyrin, transcript variant C (Ank), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175927.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D


book

Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.
Genome biology3(12)RESEARCH0085(2002)
Hoskins RA,Smith CD,Carlson JW,Carvalho AB,Halpern A,Kaminker JS,Kennedy C,Mungall CJ,Sullivan BA,Sutton GG,Yasuhara JC,Wakimoto BT,Myers EW,Celniker SE,Rubin GM,Karpen GH


book

The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective.
Genome biology3(12)RESEARCH0084(2002)
Kaminker JS,Bergman CM,Kronmiller B,Carlson J,Svirskas R,Patel S,Frise E,Wheeler DA,Lewis SE,Rubin GM,Ashburner M,Celniker SE