Ank cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Gene Symbol	Ank
Entrez Gene ID	43770
Full Name	Ankyrin
Synonyms	ANK,CG1651,CG32008,DAnk1,Dank1,Dmel\CG1651,ank,dank1
General protein information	Preferred Names Ankyrin Names CG1651 gene product from transcript CG1651-RE Ank-PB Ank-PC Ank-PD Ank-PE CG1651-PB CG1651-PC CG1651-PD CG1651-PE ankyrin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102A3-102A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_175926.3	NP_787122.1	ankyrin, isoform B
NM_001169348.2	NP_001162819.1	ankyrin, isoform E
NM_175928.3	NP_787124.1	ankyrin, isoform D
NM_175927.3	NP_787123.1	ankyrin, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ANK3	NP_001264026.1
Danio rerio (zebrafish)	ank3b	XP_005156095.1
Drosophila melanogaster (fruit fly)	Ank	NP_001162819.1
Xenopus tropicalis (tropical clawed frog)	ank3	XP_004915849.1
	ANK3	XP_002808549.1
Homo sapiens (human)	ANK3	NP_066267.2
Pan troglodytes (chimpanzee)	ANK3	XP_003312618.1
Mus musculus (house mouse)	Ank3	NP_666117.2
Rattus norvegicus (Norway rat)	Ank3	NP_001029156.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009937	XP_319063.4
Canis lupus familiaris (dog)	ANK3	XP_536358.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this study, use of osmolyte cosolvents demonstrates that hydrogen-bonding contributions can be separated from hydrophobic interactions in the denatured state of a truncated notch ankyrin protein domain.
Title: Hydrogen bonding progressively strengthens upon transfer of the protein urea-denatured state to water and protecting osmolytes.

Molecular mechanisms that enhance synapse stability despite persistent disruption of the spectrin/Ank/microtubule cytoskeleton are reported.
Title: Molecular mechanisms that enhance synapse stability despite persistent disruption of the spectrin/ankyrin/microtubule cytoskeleton.

the Spectrin network is playing a role independently of Ankyrin in the establishment and/or maintenance of specialized membrane domains containing guidance molecules that ensure the fidelity of axon repulsion at the midline
Title: beta-Spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS.

The following Ank gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ank cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00135	NM_001169348.2 Latest version!	Drosophila melanogaster ankyrin, transcript variant E (Ank), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00135	NM_175927.3 Latest version!	Drosophila melanogaster ankyrin, transcript variant C (Ank), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00135	NM_175926.3 Latest version!	Drosophila melanogaster ankyrin, transcript variant B (Ank), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00135	NM_175928.3 Latest version!	Drosophila melanogaster ankyrin, transcript variant D (Ank), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ank ( NM_175926.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787122.1 Drosophila melanogaster ankyrin, transcript variant B (Ank), mRNA.

Price & Availability↑

CloneID	OFa00135
Clone ID Related Accession (Same CDS sequence)	NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version	NM_175926.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_175926.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787122.1
CDS	27..4676
Translation	MTLGDVRVENKIQTRSETCAINGMALDNKNGIIKQNDATISFLRAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESLKIVTSTSVINSNIGAIEEKLKVMTPELMQETLLSDSDDESCDDLLDHNHYKYMATDDLKANYGQDQKNFDTTNTDHDLTDVSVLNKKEILPNEMSCIELTEIGHKPDNVVIARSQVHLGFLVSFLVDARGGSMRGYRHNGVRIIVPPKACAEPTRITCRYVKPQRVVNPPPLMEGEALVSRILEMSPVDGMFLSPITLEVPHYGTLRKNEREIIILRSDNGESWREHNLYKDIIGEDINQTEEFHSDRIVRIVTQNVPHFFAVVSRVRQEVHVIGPDGGTVFSTAVPQVKAIFPPHALTKKIRVGLQAQSVDLVECSKLLGQGVAVSPVVTVEPRRRKFHKAITLSIPAPKACTNSMVNACYGNGNSSSPTLRLLCSISGGQTRATWEDVTGSTPLSFVRDSVTFTTTVSARFWLIDCRNIIDAGRMATELYSHLAKVPFYVKFVIFAKRISQTEAKFSVFCMTDDKEDKTLEQQEYFKEVAKSRDIEVLQNQIVYLEFAGNIVPILKKGEQLYTKFQPFCENRLSFSAHIKDQEFPHGRICFMTYPMVGPDEVPLKPLCTLNISVDFKTITNHLERDNLHSLNDCINAHGKLNHNENIVFGVKEQQVKKIDITKACIMSSDIKLIHEADVILDDICSHLGSDWPLLANVLGVSQADIDLVKTEFLLNDSVKQSMAMLQLWLEHGGILTGNVLAEALYKIGRSDIVEKSFKNAEFGTHQPEKVLPTAGIEKDGDYEQ

Target ORF information:

RefSeq Version	NM_175926.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin, transcript variant B (Ank), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175926.3

ORF Insert Sequence:

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank ( NM_001169348.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162819.1 Drosophila melanogaster ankyrin, transcript variant E (Ank), mRNA.

Price & Availability↑

CloneID	OFa00135
Clone ID Related Accession (Same CDS sequence)	NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version	NM_001169348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001169348.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162819.1
CDS	69..4718
Translation	MTLGDVRVENKIQTRSETCAINGMALDNKNGIIKQNDATISFLRAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESLKIVTSTSVINSNIGAIEEKLKVMTPELMQETLLSDSDDESCDDLLDHNHYKYMATDDLKANYGQDQKNFDTTNTDHDLTDVSVLNKKEILPNEMSCIELTEIGHKPDNVVIARSQVHLGFLVSFLVDARGGSMRGYRHNGVRIIVPPKACAEPTRITCRYVKPQRVVNPPPLMEGEALVSRILEMSPVDGMFLSPITLEVPHYGTLRKNEREIIILRSDNGESWREHNLYKDIIGEDINQTEEFHSDRIVRIVTQNVPHFFAVVSRVRQEVHVIGPDGGTVFSTAVPQVKAIFPPHALTKKIRVGLQAQSVDLVECSKLLGQGVAVSPVVTVEPRRRKFHKAITLSIPAPKACTNSMVNACYGNGNSSSPTLRLLCSISGGQTRATWEDVTGSTPLSFVRDSVTFTTTVSARFWLIDCRNIIDAGRMATELYSHLAKVPFYVKFVIFAKRISQTEAKFSVFCMTDDKEDKTLEQQEYFKEVAKSRDIEVLQNQIVYLEFAGNIVPILKKGEQLYTKFQPFCENRLSFSAHIKDQEFPHGRICFMTYPMVGPDEVPLKPLCTLNISVDFKTITNHLERDNLHSLNDCINAHGKLNHNENIVFGVKEQQVKKIDITKACIMSSDIKLIHEADVILDDICSHLGSDWPLLANVLGVSQADIDLVKTEFLLNDSVKQSMAMLQLWLEHGGILTGNVLAEALYKIGRSDIVEKSFKNAEFGTHQPEKVLPTAGIEKDGDYEQ

Target ORF information:

RefSeq Version	NM_001169348.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin, transcript variant E (Ank), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169348.2

ORF Insert Sequence:

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank ( NM_175928.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787124.1 Drosophila melanogaster ankyrin, transcript variant D (Ank), mRNA.

Price & Availability↑

CloneID	OFa00135
Clone ID Related Accession (Same CDS sequence)	NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version	NM_175928.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_175928.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787124.1
CDS	217..4866
Translation	MTLGDVRVENKIQTRSETCAINGMALDNKNGIIKQNDATISFLRAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESLKIVTSTSVINSNIGAIEEKLKVMTPELMQETLLSDSDDESCDDLLDHNHYKYMATDDLKANYGQDQKNFDTTNTDHDLTDVSVLNKKEILPNEMSCIELTEIGHKPDNVVIARSQVHLGFLVSFLVDARGGSMRGYRHNGVRIIVPPKACAEPTRITCRYVKPQRVVNPPPLMEGEALVSRILEMSPVDGMFLSPITLEVPHYGTLRKNEREIIILRSDNGESWREHNLYKDIIGEDINQTEEFHSDRIVRIVTQNVPHFFAVVSRVRQEVHVIGPDGGTVFSTAVPQVKAIFPPHALTKKIRVGLQAQSVDLVECSKLLGQGVAVSPVVTVEPRRRKFHKAITLSIPAPKACTNSMVNACYGNGNSSSPTLRLLCSISGGQTRATWEDVTGSTPLSFVRDSVTFTTTVSARFWLIDCRNIIDAGRMATELYSHLAKVPFYVKFVIFAKRISQTEAKFSVFCMTDDKEDKTLEQQEYFKEVAKSRDIEVLQNQIVYLEFAGNIVPILKKGEQLYTKFQPFCENRLSFSAHIKDQEFPHGRICFMTYPMVGPDEVPLKPLCTLNISVDFKTITNHLERDNLHSLNDCINAHGKLNHNENIVFGVKEQQVKKIDITKACIMSSDIKLIHEADVILDDICSHLGSDWPLLANVLGVSQADIDLVKTEFLLNDSVKQSMAMLQLWLEHGGILTGNVLAEALYKIGRSDIVEKSFKNAEFGTHQPEKVLPTAGIEKDGDYEQ

Target ORF information:

RefSeq Version	NM_175928.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin, transcript variant D (Ank), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175928.3

ORF Insert Sequence:

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank ( NM_175927.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787123.1 Drosophila melanogaster ankyrin, transcript variant C (Ank), mRNA.

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CloneID	OFa00135
Clone ID Related Accession (Same CDS sequence)	NM_175926.3 , NM_001169348.2 , NM_175928.3 , NM_175927.3
Accession Version	NM_175927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_175927.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787123.1
CDS	102..4751
Translation	MTLGDVRVENKIQTRSETCAINGMALDNKNGIIKQNDATISFLRAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESLKIVTSTSVINSNIGAIEEKLKVMTPELMQETLLSDSDDESCDDLLDHNHYKYMATDDLKANYGQDQKNFDTTNTDHDLTDVSVLNKKEILPNEMSCIELTEIGHKPDNVVIARSQVHLGFLVSFLVDARGGSMRGYRHNGVRIIVPPKACAEPTRITCRYVKPQRVVNPPPLMEGEALVSRILEMSPVDGMFLSPITLEVPHYGTLRKNEREIIILRSDNGESWREHNLYKDIIGEDINQTEEFHSDRIVRIVTQNVPHFFAVVSRVRQEVHVIGPDGGTVFSTAVPQVKAIFPPHALTKKIRVGLQAQSVDLVECSKLLGQGVAVSPVVTVEPRRRKFHKAITLSIPAPKACTNSMVNACYGNGNSSSPTLRLLCSISGGQTRATWEDVTGSTPLSFVRDSVTFTTTVSARFWLIDCRNIIDAGRMATELYSHLAKVPFYVKFVIFAKRISQTEAKFSVFCMTDDKEDKTLEQQEYFKEVAKSRDIEVLQNQIVYLEFAGNIVPILKKGEQLYTKFQPFCENRLSFSAHIKDQEFPHGRICFMTYPMVGPDEVPLKPLCTLNISVDFKTITNHLERDNLHSLNDCINAHGKLNHNENIVFGVKEQQVKKIDITKACIMSSDIKLIHEADVILDDICSHLGSDWPLLANVLGVSQADIDLVKTEFLLNDSVKQSMAMLQLWLEHGGILTGNVLAEALYKIGRSDIVEKSFKNAEFGTHQPEKVLPTAGIEKDGDYEQ

Target ORF information:

RefSeq Version	NM_175927.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin, transcript variant C (Ank), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175927.3

ORF Insert Sequence:

ATGACGCTTG GTGATGTACG AGTTGAGAAC AAAATACAAA CAAGAAGTGA AACCTGTGCT 
ATCAATGGAA TGGCTTTAGA CAACAAGAAT GGAATCATAA AACAGAATGA CGCAACCATT 
TCGTTCTTAC GTGCTGCTCG CAGCGGAGAC ATTAAAAAGG TGATGGATTT TTTGGACTGT 
GGAGAAATAT CAGACATAAA TAGCTGCAAT GCAAACGGTC TTAATGCACT TCATTTAGCT 
GCAAAGGACG GATATGTGGA CATATGCTGC GAGTTGCTAA GAAGGGGCAT AAAGATCGAT 
AATGCCACAA AAAAGGGAAA CACAGCCTTG CACATTGCGT CTTTGGCGGG GCAGCATGAT 
GTCATCAATC AGCTAATTTT ATATAATGCT AATGTGAACG TGCAGTCTCT TAATGGTTTC 
ACCCCGCTTT ATATGGCAGC ACAAGAAAAC CACGACAATT GTTGTCGAAC CCTATTGGCC 
AATGGGGCAA ATCCGTCATT ATCTACAGAA GATGGCTTCA CACCACTTGC GGTAGCAATG 
CAGCAGGGAC ACGACAAAAT TGTAGCTGTC TTACTTGAAA ATGACGTGCG TGGGAAGGTT 
CGCCTACCAG CTCTTCATAT TGCTGCGAAA AAAAATGATG TAAATGCAGC TAAGTTATTA 
CTTCAACATG ATCCCAATGC TGACATCGTT TCCAAATCAG GATTCACCCC TTTGCACATA 
GCAGCTCATT ATGGGAACGT GGACATTGCC ACTTTGCTTT TGAACAACAA AGCAGATGTA 
AACTATGTAG CCAAACATAA CATAACTCCC TTACATGTGG CATGTAAATG GGGAAAGTTA 
TCACTTTGCA CTTTATTACT GTGCCGAGGA GCAAAAATAG ATGCGGCGAC TCGTGATGGT 
CTTACACCAC TTCACTGTGC GTCTCGCTCC GGTCATGTAG AAGTTATTAA GCATTTGTTG 
CAGCAAAACG CGCCGATACT GACAAAAACG AAGAATGGTC TGTCTGCGCT CCACATGGCT 
GCACAGGGTG AGCACGACGA AGCAGCTCAT TTATTGTTGG ACAACAAGGC TCCAGTAGAT 
GAAGTTACTG TGGATTACTT AACAGCTCTT CACGTTGCTG CTCACTGTGG TCATGTTAAA 
GTAGCTAAGC TTTTACTTGA TTACAAGGCA AATCCAAATG CCCGCGCGCT CAACGGTTTC 
ACTCCTCTTC ATATTGCGTG CAAGAAAAAT CGTATTAAGA TGGTTGAACT ACTTATTAAG 
CACGGAGCTA ACATTGGCGC AACCACGGAA TCTGGCTTAA CACCTTTACA TGTAGCTAGT 
TTTATGGGAT GTATAAATAT TGTAATATAT TTACTACAAC ATGAGGCTAG TGCGGATTTA 
CCAACAATAC GCGGAGAAAC ACCACTTCAC TTGGCAGCTC GTGCTAATCA GGCGGATATT 
ATTCGAATTT TACTTCGCAG CGCAAAAGTG GATGCTATTG CCCGTGAAGG CCAAACGCCA 
CTTCATGTAG CTTCTCGATT AGGAAATATT AATATAATTA TGTTATTACT GCAGCATGGA 
GCAGAGATAA ATGCTCAATC TAATGACAAA TATTCGGCGC TGCATATTGC TGCAAAAGAA 
GGACAAGAGA ATATAGTCCA AGTGTTACTG GAAAATGGTG CGGAAAATAA TGCTGTTACC 
AAAAAAGGGT TTACTCCACT GCATTTAGCT TGCAAATACG GAAAGCAGAA TGTGGTTCAA 
ATATTACTAC AAAATGGAGC TTCTATTGAT TTTCAGGGGA AAAATGATGT GACCCCATTA 
CACGTGGCCA CGCACTACAA TAATCCCTCA ATAGTAGAAC TCCTTTTAAA AAATGGGTCA 
TCGCCAAATT TATGTGCCCG AAATGGTCAA TGTGCTATTC ACATAGCTTG CAAAAAAAAT 
TATCTGGAAA TTGCGATGCA GCTGTTGCAA CACGGTGCAG ACGTTAATAT TATTAGCAAG 
AGTGGCTTCT CTCCGTTACA TTTGGCGGCT CAAGGGGGAA ACGTTGATAT GGTCCAACTA 
CTTCTAGAGT ATGGAGTTAT TAGCGCCGCT GCAAAAAATG GCCTAACACC ATTACATGTG 
GCTGCCCAGG AGGGCCATGT TCTTGTATCA CAGATTTTAC TGGAGCACGG TGCTAACATT 
TCAGAGCGCA CTAGGAATGG TTATACTCCC CTTCATATGG CTGCCCATTA TGGTCACCTC 
GATCTTGTCA AGTTTTTTAT TGAGAACGAC GCTGACATTG AGATGTCTTC TAATATCGGG 
TATACCCCGC TTCATCAAGC CGCTCAGCAA GGTCACATTA TGATTATTAA TCTTTTATTA 
CGCCATAAAG CGAATCCAAA TGCTTTAACA AAAGACGGGA ACACAGCTCT CCACATTGCA 
AGTAACCTTG GATACGTAAC TGTAATGGAA TCCCTCAAAA TTGTCACTTC AACATCCGTT 
ATTAATTCAA ATATTGGTGC GATTGAAGAA AAACTCAAGG TAATGACTCC AGAGCTTATG 
CAAGAAACCT TACTTTCCGA TTCCGATGAC GAATCTTGTG ATGATCTTTT GGATCATAAC 
CATTACAAAT ACATGGCAAC TGATGATTTA AAAGCCAACT ACGGCCAGGA TCAGAAGAAC 
TTCGATACTA CAAACACCGA CCACGATCTT ACTGATGTTT CAGTACTAAA TAAGAAAGAA 
ATACTACCCA ATGAAATGAG CTGTATTGAA TTGACTGAAA TCGGGCACAA ACCCGATAAC 
GTTGTTATTG CCCGATCCCA AGTTCATTTA GGGTTTCTGG TGTCGTTCTT GGTAGATGCA 
CGTGGCGGAT CCATGCGCGG ATATCGGCAC AATGGTGTGC GCATTATCGT ACCTCCAAAG 
GCCTGCGCTG AACCAACACG AATAACATGT CGATATGTTA AGCCGCAAAG GGTAGTCAAC 
CCTCCGCCTT TAATGGAGGG TGAAGCACTT GTGAGTCGCA TTTTGGAAAT GTCACCTGTT 
GACGGAATGT TTTTAAGTCC AATAACTTTA GAAGTTCCAC ACTATGGCAC CCTTAGAAAA 
AACGAGCGCG AAATTATTAT ACTCCGGTCC GACAATGGAG AAAGTTGGCG TGAGCATAAC 
TTATATAAAG ACATAATAGG TGAAGATATA AACCAAACGG AGGAATTTCA TTCGGATCGC 
ATCGTTCGGA TCGTTACTCA AAATGTTCCG CACTTTTTTG CTGTTGTTTC GCGAGTTCGT 
CAGGAAGTGC ACGTTATTGG ACCTGATGGT GGAACCGTTT TCTCTACAGC AGTGCCCCAG 
GTCAAAGCTA TATTTCCGCC TCATGCTTTA ACTAAAAAAA TTCGAGTCGG TCTTCAGGCT 
CAGTCAGTAG ACCTGGTTGA ATGTTCTAAA CTGCTCGGTC AAGGGGTCGC TGTCTCACCC 
GTAGTAACTG TGGAGCCACG CCGTCGAAAA TTTCACAAAG CTATTACATT AAGCATTCCA 
GCTCCAAAAG CATGTACAAA TAGTATGGTT AATGCATGTT ATGGCAATGG AAATTCAAGT 
TCGCCTACTC TTCGACTTTT GTGTTCCATT AGTGGTGGTC AAACCCGAGC AACATGGGAG 
GATGTTACTG GATCAACACC GTTGTCATTT GTTAGAGACA GTGTAACATT TACCACTACT 
GTTTCTGCCC GGTTTTGGCT AATAGATTGC CGTAATATCA TAGACGCAGG ACGAATGGCC 
ACAGAACTTT ATAGTCATTT GGCCAAAGTT CCTTTTTATG TCAAGTTTGT AATATTTGCA 
AAACGAATTT CCCAAACGGA AGCAAAATTT TCAGTATTTT GCATGACTGA TGATAAAGAG 
GATAAGACCC TTGAGCAACA AGAATATTTT AAAGAAGTTG CCAAAAGCAG GGATATCGAA 
GTATTGCAAA ATCAAATTGT ATATCTTGAA TTCGCTGGTA ACATTGTTCC CATTTTAAAG 
AAAGGCGAAC AGCTGTATAC CAAGTTTCAA CCTTTTTGCG AAAACCGGCT TTCCTTTAGT 
GCTCACATTA AAGATCAAGA ATTTCCACAT GGTCGCATCT GCTTTATGAC CTACCCGATG 
GTAGGACCAG ATGAAGTGCC CCTGAAGCCG CTTTGTACTC TGAACATTTC AGTGGATTTT 
AAAACTATAA CAAACCATCT CGAAAGAGAT AACCTGCATA GCTTGAACGA TTGTATAAAT 
GCGCATGGAA AATTAAACCA CAATGAAAAT ATTGTATTTG GAGTAAAGGA ACAACAGGTG 
AAGAAAATTG ATATTACTAA AGCTTGTATA ATGTCAAGTG ACATCAAACT AATTCATGAG 
GCTGATGTAA TATTAGACGA CATATGTAGC CACTTAGGAA GTGACTGGCC TTTGCTTGCA 
AATGTATTGG GTGTTTCCCA AGCCGATATC GATCTTGTTA AAACAGAATT TTTGTTAAAC 
GACAGCGTAA AGCAGAGCAT GGCAATGTTA CAGCTATGGC TAGAACATGG AGGCATATTG 
ACGGGCAATG TACTAGCTGA AGCCCTCTAC AAAATCGGCC GCTCAGATAT AGTGGAGAAA 
AGCTTTAAAA ATGCAGAGTT TGGCACTCAT CAACCGGAAA AAGTTTTGCC GACTGCCGGA 
ATTGAAAAAG ATGGCGATTA TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ank cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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