SmD1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Gene Symbol	SmD1
Entrez Gene ID	44668
Full Name	Small ribonucleoprotein particle protein SmD1
Synonyms	BcDNA:RE39488,CG10753,D1-Sm,Dmel\CG10753,dD1,l(2)SH3 003,l(3)SH003,smd1,snRNP,snRNP-69D,snRNP69D
General protein information	Preferred Names Small ribonucleoprotein particle protein SmD1 Names CG10753 gene product from transcript CG10753-RA CG10753-PA SmD1-PA lethal (3) SH003 small nuclear ribonucleoprotein at 69D small ribonucleoprotein particle protein SmD1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69D3-69D3

mRNA and Protein(s)

mRNA Protein Name

NM_080035.3 NP_524774.1 small ribonucleoprotein particle protein SmD1
Pathways from BioSystems

mRNA	Protein	Name
NM_080035.3	NP_524774.1	small ribonucleoprotein particle protein SmD1

Homologies

Pan troglodytes (chimpanzee)	SNRPD1	XP_512054.2
Mus musculus (house mouse)	Snrpd1	NP_033252.1
Caenorhabditis elegans (roundworm)	snr-3	NP_495306.1
Arabidopsis thaliana (thale cress)	AT3G07590	NP_001189837.1
Danio rerio (zebrafish)	snrpd1	NP_775359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007246	XP_308555.4
Magnaporthe oryzae (rice blast fungus)	MGG_04741	XP_003713702.1
Homo sapiens (human)	SNRPD1	NP_008869.1
Rattus norvegicus (Norway rat)	Snrpd1	NP_001099633.1
Xenopus tropicalis (tropical clawed frog)	snrpd1	NP_001004780.1
	SNRPD1	XP_001091972.1
Canis lupus familiaris (dog)	SNRPD1	XP_537302.1
Drosophila melanogaster (fruit fly)	SmD1	NP_524774.1
Schizosaccharomyces pombe (fission yeast)	smd1	NP_594613.1
Arabidopsis thaliana (thale cress)	AT4G02840	NP_192193.1
Oryza sativa (rice)	Os02g0586500	NP_001047268.1
Oryza sativa (rice)	Os04g0470200	NP_001053049.2
Bos taurus (cattle)	SNRPD1	NP_001030237.1
Gallus gallus (chicken)	SNRPD1	NP_001264927.1
Neurospora crassa	NCU00505	XP_964309.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SmD1 plays a direct role in miRNA-mediated gene silencing independently of its pre-mRNA splicing activity and indicates that the dual roles of splicing factors in post-transcriptional gene regulation may be evolutionarily widespread.
Title: SmD1 Modulates the miRNA Pathway Independently of Its Pre-mRNA Splicing Function.

Drosophila SmD1 plays a direct role in RNAi-mediated gene silencing independently of its pre-mRNA splicing activity.
Title: Core small nuclear ribonucleoprotein particle splicing factor SmD1 modulates RNA interference in Drosophila.

The following SmD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SmD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20486	NM_080035.3 Latest version!	Drosophila melanogaster small ribonucleoprotein particle protein SmD1 (SmD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SmD1 ( NM_080035.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524774.1 Drosophila melanogaster small ribonucleoprotein particle protein SmD1 (SmD1), mRNA.

Price & Availability↑

CloneID	OFa20486
Clone ID Related Accession (Same CDS sequence)	NM_080035.3
Accession Version	NM_080035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	small ribonucleoprotein particle protein SmD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080035.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATTAG TAAGATTCCT TATGAAGCTA AGTCACGAGA CCGTGACCAT CGAACTGAAG 
AACGGCACCC AGATCCACGG CACCATTACC GGCGTGGATG TGGCCATGAA CACTCACCTG 
AAGAGCGTTC GGATGACGAT CAAGAACCGG GATCCCGTCC ACCTGGAGAC GCTGAGCATT 
CGCGGCAACA ACATCAGATA CTTTATACTG CCGGACAGCC TGCCGCTGGA GACGCTCCTC 
ATCGACGACA CCCCGAAGTC GAAGACAAAA AAGAAGGACA GCGGCCGCGT GGGAAATCGC 
GGCAGGGGCA GAGGCGCCCG CGGACGAGGT GGTCCACGGG GTCGCGGAAG GGGCCGAGCT 
TCAGGCCGAC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524774.1
CDS	96..470
Translation	MKLVRFLMKLSHETVTIELKNGTQIHGTITGVDVAMNTHLKSVRMTIKNRDPVHLETLSIRGNNIRYFILPDSLPLETLLIDDTPKSKTKKKDSGRVGNRGRGRGARGRGGPRGRGRGRASGRR

Target ORF information:

RefSeq Version	NM_080035.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster small ribonucleoprotein particle protein SmD1 (SmD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080035.3

ORF Insert Sequence:

ATGAAATTAG TAAGATTCCT TATGAAGCTA AGTCACGAGA CCGTGACCAT CGAACTGAAG 
AACGGCACCC AGATCCACGG CACCATTACC GGCGTGGATG TGGCCATGAA CACTCACCTG 
AAGAGCGTTC GGATGACGAT CAAGAACCGG GATCCCGTCC ACCTGGAGAC GCTGAGCATT 
CGCGGCAACA ACATCAGATA CTTTATACTG CCGGACAGCC TGCCGCTGGA GACGCTCCTC 
ATCGACGACA CCCCGAAGTC GAAGACAAAA AAGAAGGACA GCGGCCGCGT GGGAAATCGC 
GGCAGGGGCA GAGGCGCCCG CGGACGAGGT GGTCCACGGG GTCGCGGAAG GGGCCGAGCT 
TCAGGCCGAC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SmD1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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