bel cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bel
Entrez Gene ID	45826
Full Name	belle
Synonyms	BEL,Bel,CG9748,DmRH6,Dmel\CG9748,L3,anon-85Ab,cap,cg9748,l(3)85Ac,l(3)L3,l(3)L4740,l(3)S047110,l(3)S097074,ms(3)Pneo85A,ms(3)neo30,psg9
General protein information	Preferred Names belle Names CG9748 gene product from transcript CG9748-RB CG9748-PA CG9748-PB bel-PA bel-PB capon lethal (3) L4740 lethal (3) S047110
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85A-85A5\|3-44.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275450.1	NP_001262379.1	belle, isoform B
NM_080522.4	NP_536783.1	belle, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DDX3X	XP_005641313.1
Drosophila melanogaster (fruit fly)	bel	NP_536783.1
Gallus gallus (chicken)	DDX3X	NP_001025971.1
Arabidopsis thaliana (thale cress)	AT2G42520	NP_181780.1
Homo sapiens (human)	DDX3X	NP_001180345.1
Mus musculus (house mouse)	Ddx3x	NP_034158.1
Arabidopsis thaliana (thale cress)	AT3G58510	NP_001030884.1
Oryza sativa (rice)	Os03g0805200	NP_001051626.1
Caenorhabditis elegans (roundworm)	laf-1	NP_001254858.1
Oryza sativa (rice)	Os07g0202100	NP_001059135.1
Xenopus tropicalis (tropical clawed frog)	ddx3x	NP_989196.1
Bos taurus (cattle)	DDX3X	NP_001179891.1
Arabidopsis thaliana (thale cress)	AT3G58570	NP_191416.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bel is required for nos mRNA translational repression.
Title: Translational repression of the

Results suggest that a role of RNA helicase Belle in germline stem cells (GSCs) and maintenance and germline stem cells (GSCs)regulation of early germ cell divisions.
Title: RNA helicase Belle (DDX3) is essential for male germline stem cell maintenance and division in Drosophila.

show that genetic inhibition of Bel function leads to de novo generation of piRNAs from the transgene region inserted in the genome, suggesting a potential piRNA-dependent mechanism that may mediate transgene silencing as Bel function is inhibited
Title: RNA helicase Belle/DDX3 regulates transgene expression in Drosophila.

DDX3/Belle Exerts Its Function Outside of the Wnt/Wingless Signaling Pathway
Title: Drosophila DDX3/Belle Exerts Its Function Outside of the Wnt/Wingless Signaling Pathway.

findings show that the belle (bel) mRNA is translationally repressed by the d4EHP protein in the Drosophila ovary. Consistent with this regulation, d4EHP overexpression in the ovary phenocopies the bel mutant.
Title: Patterning of the Drosophila oocyte by a sequential translation repression program involving the d4EHP and Belle translational repressors.

The following bel gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bel cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06895	NM_080522.4 Latest version!	Drosophila melanogaster belle (bel), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa07614	NM_001275450.1 Latest version!	Drosophila melanogaster belle (bel), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bel ( NM_080522.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536783.1 Drosophila melanogaster belle (bel), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06895
Clone ID Related Accession (Same CDS sequence)	NM_080522.4
Accession Version	NM_080522.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	belle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080522.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAATG CTATTAACCA AAATGGCACA GGTCTAGAGC AGCAAGTTGC TGGTCTGGAC 
TTGAATGGCG GCTCTGCTGA CTACAGCGGC CCCATAACCA GCAAGACTTC CACTAACTCG 
GTCACCGGTG GTGTGTATGT GCCCCCGCAC CTTCGTGGTG GTGGTGGCAA TAACAACGCA 
GCGGACGCAG AGAGCCAGGG CCAAGGACAG GGCCAGGGTC AGGGCTTCGA CTCCAGATCC 
GGGAATCCGC GCCAGGAGAC TAGGGACCCT CAACAGTCGC GCGGAGGCGG AGGCGAATAC 
CGCAGAGGCG GCGGCGGTGG TGGTCGCGGC TTCAATCGCC AGAGCGGCGA CTACGGTTAC 
GGAAGCGGCG GCGGAGGACG ACGTGGAGGC GGTGGACGTT TCGAGGACAA CTACAACGGT 
GGTGAATTCG ATTCGCGTCG CGGCGGTGAC TGGAATCGCA GCGGCGGAGG CGGCGGAGGA 
GGTCGAGGAT TTGGACGCGG ACCATCGTAC CGCGGCGGTG GCGGCGGAAG CGGTAGCAAC 
CTCAACGAGC AGACTGCCGA GGATGGCCAG GCACAGCAGC AGCAGCAGCC GCGCAACGAT 
CGTTGGCAGG AGCCAGAACG TCCTGCCGGA TTCGATGGCA GCGAGGGCGG ACAGTCTGCC 
GGCGGCAACA GAAGCTACAA CAATCGCGGC GAGCGCGGAG GCGGCGGTTA CAACAGCCGC 
TGGAAGGAGG GCGGCGGCTC AAACGTCGAC TACACAAAGC TGGGCGCCCG AGATGAGCGC 
CTGGAGGTGG AGCTGTTCGG CGTGGGCAAT ACGGGAATCA ATTTTGACAA ATACGAGGAT 
ATACCCGTGG AGGCGACGGG CCAGAATGTG CCCCCGAACA TCACATCGTT CGATGATGTG 
CAGCTGACGG AGATTATCCG CAACAACGTG GCCTTAGCAC GTTATGACAA ACCGACACCG 
GTGCAGAAGC ACGCCATTCC GATCATTATC AATGGCCGGG ATCTAATGGC CTGCGCCCAG 
ACTGGATCCG GCAAGACGGC CGCCTTCCTG GTGCCGATTC TCAACCAGAT GTACGAGCTG 
GGACATGTGC CCCCGCCGCA AAGCACCCGG CAGTACAGCC GGCGCAAGCA GTATCCGTTG 
GGTCTGGTGC TGGCGCCGAC CCGCGAACTG GCCACCCAGA TCTTTGAGGA GGCCAAGAAG 
TTCGCCTATC GCTCCCGGAT GCGCCCGGCA GTGCTGTACG GCGGAAACAA TACTAGCGAG 
CAGATGCGCG AGCTGGACCG CGGTTGCCAT CTGATTGTGG CCACACCCGG TCGCCTAGAG 
GACATGATCA CACGCGGAAA GGTGGGACTG GAGAACATTC GATTCCTTGT ACTGGATGAG 
GCTGATCGTA TGTTGGACAT GGGTTTCGAG CCGCAGATCC GTCGCATCGT CGAACAGCTA 
AACATGCCGC CAACCGGACA GCGCCAGACG CTTATGTTCT CGGCCACATT CCCCAAGCAG 
ATCCAGGAGT TAGCCAGCGA TTTCCTCAGC AACTACATTT TCTTGGCCGT TGGTCGTGTG 
GGCTCCACAT CTGAGAACAT TACGCAGACA ATCCTGTGGG TCTATGAACC CGACAAGCGC 
TCGTATCTGC TGGATCTGCT CTCGTCTATC CGCGATGGCC CCGAATACAC CAAAGACAGC 
CTAACACTGA TCTTTGTGGA GACTAAGAAG GGTGCAGACT CGCTGGAGGA GTTCCTGTAC 
CAGTGCAATC ATCCCGTCAC TAGCATCCAC GGTGATCGCA CGCAGAAGGA GCGCGAGGAG 
GCACTGCGCT GCTTCCGATC GGGCGACTGC CCCATCCTGG TGGCCACAGC CGTGGCCGCT 
CGTGGCCTGG ACATTCCGCA CGTTAAGCAC GTTATTAACT TTGACCTGCC CTCGGATGTG 
GAGGAGTATG TCCACCGTAT CGGGCGTACC GGACGTATGG GTAATCTTGG TGTGGCCACC 
TCCTTTTTCA ACGAAAAGAA CCGCAACATT TGCTCCGATC TCTTGGAGCT GCTGATCGAA 
ACGAAGCAGG AGATCCCCAG TTTCATGGAG GATATGAGTT CGGATCGCGG ACATGGCGGA 
GCCAAGCGGG CTGGACGAGG CGGTGGTGGA CGTTATGGTG GCGGCTTCGG CTCCAGGGAT 
TACCGTCAAA GCTCTGGCGG CGGCGGTGGC GGACGCAGTG GACCGCCACC ACGTAGCGGT 
GGTTCCGGAT CAGGAGGCGG CGGCGGCAGT TACCGCAGCA ATGGAAACTC GTACGGCGGC 
AATTCGGGCG GCGGTGGATA CTATGGCGGC GGCGCCGGTG GTGGCTCCTA TGGCGGCTCC 
TACGGCGGCG GCAGTGCCTC GCACTCTTCG AACGCACCCG ACTGGTGGGC TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536783.1
CDS	416..2812
Translation	MSNAINQNGTGLEQQVAGLDLNGGSADYSGPITSKTSTNSVTGGVYVPPHLRGGGGNNNAADAESQGQGQGQGQGFDSRSGNPRQETRDPQQSRGGGGEYRRGGGGGGRGFNRQSGDYGYGSGGGGRRGGGGRFEDNYNGGEFDSRRGGDWNRSGGGGGGGRGFGRGPSYRGGGGGSGSNLNEQTAEDGQAQQQQQPRNDRWQEPERPAGFDGSEGGQSAGGNRSYNNRGERGGGGYNSRWKEGGGSNVDYTKLGARDERLEVELFGVGNTGINFDKYEDIPVEATGQNVPPNITSFDDVQLTEIIRNNVALARYDKPTPVQKHAIPIIINGRDLMACAQTGSGKTAAFLVPILNQMYELGHVPPPQSTRQYSRRKQYPLGLVLAPTRELATQIFEEAKKFAYRSRMRPAVLYGGNNTSEQMRELDRGCHLIVATPGRLEDMITRGKVGLENIRFLVLDEADRMLDMGFEPQIRRIVEQLNMPPTGQRQTLMFSATFPKQIQELASDFLSNYIFLAVGRVGSTSENITQTILWVYEPDKRSYLLDLLSSIRDGPEYTKDSLTLIFVETKKGADSLEEFLYQCNHPVTSIHGDRTQKEREEALRCFRSGDCPILVATAVAARGLDIPHVKHVINFDLPSDVEEYVHRIGRTGRMGNLGVATSFFNEKNRNICSDLLELLIETKQEIPSFMEDMSSDRGHGGAKRAGRGGGGRYGGGFGSRDYRQSSGGGGGGRSGPPPRSGGSGSGGGGGSYRSNGNSYGGNSGGGGYYGGGAGGGSYGGSYGGGSASHSSNAPDWWAQ

Target ORF information:

RefSeq Version	NM_080522.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster belle (bel), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080522.4

ORF Insert Sequence:

ATGAGTAATG CTATTAACCA AAATGGCACA GGTCTAGAGC AGCAAGTTGC TGGTCTGGAC 
TTGAATGGCG GCTCTGCTGA CTACAGCGGC CCCATAACCA GCAAGACTTC CACTAACTCG 
GTCACCGGTG GTGTGTATGT GCCCCCGCAC CTTCGTGGTG GTGGTGGCAA TAACAACGCA 
GCGGACGCAG AGAGCCAGGG CCAAGGACAG GGCCAGGGTC AGGGCTTCGA CTCCAGATCC 
GGGAATCCGC GCCAGGAGAC TAGGGACCCT CAACAGTCGC GCGGAGGCGG AGGCGAATAC 
CGCAGAGGCG GCGGCGGTGG TGGTCGCGGC TTCAATCGCC AGAGCGGCGA CTACGGTTAC 
GGAAGCGGCG GCGGAGGACG ACGTGGAGGC GGTGGACGTT TCGAGGACAA CTACAACGGT 
GGTGAATTCG ATTCGCGTCG CGGCGGTGAC TGGAATCGCA GCGGCGGAGG CGGCGGAGGA 
GGTCGAGGAT TTGGACGCGG ACCATCGTAC CGCGGCGGTG GCGGCGGAAG CGGTAGCAAC 
CTCAACGAGC AGACTGCCGA GGATGGCCAG GCACAGCAGC AGCAGCAGCC GCGCAACGAT 
CGTTGGCAGG AGCCAGAACG TCCTGCCGGA TTCGATGGCA GCGAGGGCGG ACAGTCTGCC 
GGCGGCAACA GAAGCTACAA CAATCGCGGC GAGCGCGGAG GCGGCGGTTA CAACAGCCGC 
TGGAAGGAGG GCGGCGGCTC AAACGTCGAC TACACAAAGC TGGGCGCCCG AGATGAGCGC 
CTGGAGGTGG AGCTGTTCGG CGTGGGCAAT ACGGGAATCA ATTTTGACAA ATACGAGGAT 
ATACCCGTGG AGGCGACGGG CCAGAATGTG CCCCCGAACA TCACATCGTT CGATGATGTG 
CAGCTGACGG AGATTATCCG CAACAACGTG GCCTTAGCAC GTTATGACAA ACCGACACCG 
GTGCAGAAGC ACGCCATTCC GATCATTATC AATGGCCGGG ATCTAATGGC CTGCGCCCAG 
ACTGGATCCG GCAAGACGGC CGCCTTCCTG GTGCCGATTC TCAACCAGAT GTACGAGCTG 
GGACATGTGC CCCCGCCGCA AAGCACCCGG CAGTACAGCC GGCGCAAGCA GTATCCGTTG 
GGTCTGGTGC TGGCGCCGAC CCGCGAACTG GCCACCCAGA TCTTTGAGGA GGCCAAGAAG 
TTCGCCTATC GCTCCCGGAT GCGCCCGGCA GTGCTGTACG GCGGAAACAA TACTAGCGAG 
CAGATGCGCG AGCTGGACCG CGGTTGCCAT CTGATTGTGG CCACACCCGG TCGCCTAGAG 
GACATGATCA CACGCGGAAA GGTGGGACTG GAGAACATTC GATTCCTTGT ACTGGATGAG 
GCTGATCGTA TGTTGGACAT GGGTTTCGAG CCGCAGATCC GTCGCATCGT CGAACAGCTA 
AACATGCCGC CAACCGGACA GCGCCAGACG CTTATGTTCT CGGCCACATT CCCCAAGCAG 
ATCCAGGAGT TAGCCAGCGA TTTCCTCAGC AACTACATTT TCTTGGCCGT TGGTCGTGTG 
GGCTCCACAT CTGAGAACAT TACGCAGACA ATCCTGTGGG TCTATGAACC CGACAAGCGC 
TCGTATCTGC TGGATCTGCT CTCGTCTATC CGCGATGGCC CCGAATACAC CAAAGACAGC 
CTAACACTGA TCTTTGTGGA GACTAAGAAG GGTGCAGACT CGCTGGAGGA GTTCCTGTAC 
CAGTGCAATC ATCCCGTCAC TAGCATCCAC GGTGATCGCA CGCAGAAGGA GCGCGAGGAG 
GCACTGCGCT GCTTCCGATC GGGCGACTGC CCCATCCTGG TGGCCACAGC CGTGGCCGCT 
CGTGGCCTGG ACATTCCGCA CGTTAAGCAC GTTATTAACT TTGACCTGCC CTCGGATGTG 
GAGGAGTATG TCCACCGTAT CGGGCGTACC GGACGTATGG GTAATCTTGG TGTGGCCACC 
TCCTTTTTCA ACGAAAAGAA CCGCAACATT TGCTCCGATC TCTTGGAGCT GCTGATCGAA 
ACGAAGCAGG AGATCCCCAG TTTCATGGAG GATATGAGTT CGGATCGCGG ACATGGCGGA 
GCCAAGCGGG CTGGACGAGG CGGTGGTGGA CGTTATGGTG GCGGCTTCGG CTCCAGGGAT 
TACCGTCAAA GCTCTGGCGG CGGCGGTGGC GGACGCAGTG GACCGCCACC ACGTAGCGGT 
GGTTCCGGAT CAGGAGGCGG CGGCGGCAGT TACCGCAGCA ATGGAAACTC GTACGGCGGC 
AATTCGGGCG GCGGTGGATA CTATGGCGGC GGCGCCGGTG GTGGCTCCTA TGGCGGCTCC 
TACGGCGGCG GCAGTGCCTC GCACTCTTCG AACGCACCCG ACTGGTGGGC TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bel ( NM_001275450.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262379.1 Drosophila melanogaster belle (bel), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07614
Clone ID Related Accession (Same CDS sequence)	NM_001275450.1
Accession Version	NM_001275450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	belle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAATG CTATTAACCA AAATGGCACA GGTCTAGAGC AGCAAGTTGC TGGTCTGGAC 
TTGAATGGCG GCTCTGCTGA CTACAGCGGC CCCATAACCA GCAAGACTTC CACTAACTCG 
GTCACCGGTG GTGTGTATGT GCCCCCGCAC CTTCGTGGTG GTGGTGGCAA TAACAACGCA 
GCGGACGCAG AGAGCCAGGG CCAAGGACAG GGCCAGGGTC AGGGCTTCGA CTCCAGATCC 
GGGAATCCGC GCCAGGAGAC TAGGGACCCT CAACAGTCGC GCGGAGGCGG AGGCGAATAC 
CGCAGAGGCG GCGGCGGTGG TGGTCGCGGC TTCAATCGCC AGAGCGGCGA CTACGGTTAC 
GGAAGCGGCG GCGGAGGACG ACGTGGAGGC GGTGGACGTT TCGAGGACAA CTACAACGGT 
GGTGAATTCG ATTCGCGTCG CGGCGGTGAC TGGAATCGCA GCGGCGGAGG CGGCGGAGGA 
GGTCGAGGAT TTGGACGCGG ACCATCGTAC CGCGGCGGTG GCGGCGGAAG CGGTAGCAAC 
CTCAACGAGC AGACTGCCGA GGATGGCCAG GCACAGCAGC AGCAGCAGCC GCGCAACGAT 
CGTTGGCAGG AGCCAGAACG TCCTGCCGGA TTCGATGGCA GCGAGGGCGG ACAGTCTGCC 
GGCGGCAACA GAAGCTACAA CAATCGCGGC GAGCGCGGAG GCGGCGGTTA CAACAGCCGC 
TGGAAGGAGG GCGGCGGCTC AAACGTCGAC TACACAAAGC TGGGCGCCCG AGATGAGCGC 
CTGGAGGTGG AGCTGTTCGG CGTGGGCAAT ACGGGAATCA ATTTTGACAA ATACGAGGAT 
ATACCCGTGG AGGCGACGGG CCAGAATGTG CCCCCGAACA TCACATCGTT CGATGATGTG 
CAGCTGACGG AGATTATCCG CAACAACGTG GCCTTAGCAC GTTATGACAA ACCGACACCG 
GTGCAGAAGC ACGCCATTCC GATCATTATC AATGGCCGGG ATCTAATGGC CTGCGCCCAG 
ACTGGATCCG GCAAGACGGC CGCCTTCCTG GTGCCGATTC TCAACCAGAT GTACGAGCTG 
GGACATGTGC CCCCGCCGCA AAGCACCCGG CAGTACAGCC GGCGCAAGCA GTATCCGTTG 
GGTCTGGTGC TGGCGCCGAC CCGCGAACTG GCCACCCAGA TCTTTGAGGA GGCCAAGAAG 
TTCGCCTATC GCTCCCGGAT GCGCCCGGCA GTGCTGTACG GCGGAAACAA TACTAGCGAG 
CAGATGCGCG AGCTGGACCG CGGTTGCCAT CTGATTGTGG CCACACCCGG TCGCCTAGAG 
GACATGATCA CACGCGGAAA GGTGGGACTG GAGAACATTC GATTCCTTGT ACTGGATGAG 
GCTGATCGTA TGTTGGACAT GGGTTTCGAG CCGCAGATCC GTCGCATCGT CGAACAGCTA 
AACATGCCGC CAACCGGACA GCGCCAGACG CTTATGTTCT CGGCCACATT CCCCAAGCAG 
ATCCAGGAGT TAGCCAGCGA TTTCCTCAGC AACTACATTT TCTTGGCCGT TGGTCGTGTG 
GGCTCCACAT CTGAGAACAT TACGCAGACA ATCCTGTGGG TCTATGAACC CGACAAGCGC 
TCGTATCTGC TGGATCTGCT CTCGTCTATC CGCGATGGCC CCGAATACAC CAAAGACAGC 
CTAACACTGA TCTTTGTGGA GACTAAGAAG GGTGCAGACT CGCTGGAGGA GTTCCTGTAC 
CAGTGCAATC ATCCCGTCAC TAGCATCCAC GGTGATCGCA CGCAGAAGGA GCGCGAGGAG 
GCACTGCGCT GCTTCCGATC GGGCGACTGC CCCATCCTGG TGGCCACAGC CGTGGCCGCT 
CGTGGCCTGG ACATTCCGCA CGTTAAGCAC GTTATTAACT TTGACCTGCC CTCGGATGTG 
GAGGAGTATG TCCACCGTAT CGGGCGTACC GGACGTATGG GTAATCTTGG TGTGGCCACC 
TCCTTTTTCA ACGAAAAGAA CCGCAACATT TGCTCCGATC TCTTGGAGCT GCTGATCGAA 
ACGAAGCAGG AGATCCCCAG TTTCATGGAG GATATGAGTT CGGATCGCGG ACATGGCGGA 
GCCAAGCGGG CTGGACGAGG CGGTGGTGGA CGTTATGGTG GCGGCTTCGG CTCCAGGGAT 
TACCGTCAAA GCTCTGGCGG CGGCGGTGGC GGACGCAGTG GACCGCCACC ACGTAGCGGT 
GGTTCCGGAT CAGGAGGCGG CGGCGGCAGT TACCGCAGCA ATGGAAACTC GTACGGTAAG 
TTTGGCGGCA ATTCGGGCGG CGGTGGATAC TATGGCGGCG GCGCCGGTGG TGGCTCCTAT 
GGCGGCTCCT ACGGCGGCGG CAGTGCCTCG CACTCTTCGA ACGCACCCGA CTGGTGGGCT 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262379.1
CDS	755..3160
Translation	MSNAINQNGTGLEQQVAGLDLNGGSADYSGPITSKTSTNSVTGGVYVPPHLRGGGGNNNAADAESQGQGQGQGQGFDSRSGNPRQETRDPQQSRGGGGEYRRGGGGGGRGFNRQSGDYGYGSGGGGRRGGGGRFEDNYNGGEFDSRRGGDWNRSGGGGGGGRGFGRGPSYRGGGGGSGSNLNEQTAEDGQAQQQQQPRNDRWQEPERPAGFDGSEGGQSAGGNRSYNNRGERGGGGYNSRWKEGGGSNVDYTKLGARDERLEVELFGVGNTGINFDKYEDIPVEATGQNVPPNITSFDDVQLTEIIRNNVALARYDKPTPVQKHAIPIIINGRDLMACAQTGSGKTAAFLVPILNQMYELGHVPPPQSTRQYSRRKQYPLGLVLAPTRELATQIFEEAKKFAYRSRMRPAVLYGGNNTSEQMRELDRGCHLIVATPGRLEDMITRGKVGLENIRFLVLDEADRMLDMGFEPQIRRIVEQLNMPPTGQRQTLMFSATFPKQIQELASDFLSNYIFLAVGRVGSTSENITQTILWVYEPDKRSYLLDLLSSIRDGPEYTKDSLTLIFVETKKGADSLEEFLYQCNHPVTSIHGDRTQKEREEALRCFRSGDCPILVATAVAARGLDIPHVKHVINFDLPSDVEEYVHRIGRTGRMGNLGVATSFFNEKNRNICSDLLELLIETKQEIPSFMEDMSSDRGHGGAKRAGRGGGGRYGGGFGSRDYRQSSGGGGGGRSGPPPRSGGSGSGGGGGSYRSNGNSYGKFGGNSGGGGYYGGGAGGGSYGGSYGGGSASHSSNAPDWWAQ

Target ORF information:

RefSeq Version	NM_001275450.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster belle (bel), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275450.1

ORF Insert Sequence:

ATGAGTAATG CTATTAACCA AAATGGCACA GGTCTAGAGC AGCAAGTTGC TGGTCTGGAC 
TTGAATGGCG GCTCTGCTGA CTACAGCGGC CCCATAACCA GCAAGACTTC CACTAACTCG 
GTCACCGGTG GTGTGTATGT GCCCCCGCAC CTTCGTGGTG GTGGTGGCAA TAACAACGCA 
GCGGACGCAG AGAGCCAGGG CCAAGGACAG GGCCAGGGTC AGGGCTTCGA CTCCAGATCC 
GGGAATCCGC GCCAGGAGAC TAGGGACCCT CAACAGTCGC GCGGAGGCGG AGGCGAATAC 
CGCAGAGGCG GCGGCGGTGG TGGTCGCGGC TTCAATCGCC AGAGCGGCGA CTACGGTTAC 
GGAAGCGGCG GCGGAGGACG ACGTGGAGGC GGTGGACGTT TCGAGGACAA CTACAACGGT 
GGTGAATTCG ATTCGCGTCG CGGCGGTGAC TGGAATCGCA GCGGCGGAGG CGGCGGAGGA 
GGTCGAGGAT TTGGACGCGG ACCATCGTAC CGCGGCGGTG GCGGCGGAAG CGGTAGCAAC 
CTCAACGAGC AGACTGCCGA GGATGGCCAG GCACAGCAGC AGCAGCAGCC GCGCAACGAT 
CGTTGGCAGG AGCCAGAACG TCCTGCCGGA TTCGATGGCA GCGAGGGCGG ACAGTCTGCC 
GGCGGCAACA GAAGCTACAA CAATCGCGGC GAGCGCGGAG GCGGCGGTTA CAACAGCCGC 
TGGAAGGAGG GCGGCGGCTC AAACGTCGAC TACACAAAGC TGGGCGCCCG AGATGAGCGC 
CTGGAGGTGG AGCTGTTCGG CGTGGGCAAT ACGGGAATCA ATTTTGACAA ATACGAGGAT 
ATACCCGTGG AGGCGACGGG CCAGAATGTG CCCCCGAACA TCACATCGTT CGATGATGTG 
CAGCTGACGG AGATTATCCG CAACAACGTG GCCTTAGCAC GTTATGACAA ACCGACACCG 
GTGCAGAAGC ACGCCATTCC GATCATTATC AATGGCCGGG ATCTAATGGC CTGCGCCCAG 
ACTGGATCCG GCAAGACGGC CGCCTTCCTG GTGCCGATTC TCAACCAGAT GTACGAGCTG 
GGACATGTGC CCCCGCCGCA AAGCACCCGG CAGTACAGCC GGCGCAAGCA GTATCCGTTG 
GGTCTGGTGC TGGCGCCGAC CCGCGAACTG GCCACCCAGA TCTTTGAGGA GGCCAAGAAG 
TTCGCCTATC GCTCCCGGAT GCGCCCGGCA GTGCTGTACG GCGGAAACAA TACTAGCGAG 
CAGATGCGCG AGCTGGACCG CGGTTGCCAT CTGATTGTGG CCACACCCGG TCGCCTAGAG 
GACATGATCA CACGCGGAAA GGTGGGACTG GAGAACATTC GATTCCTTGT ACTGGATGAG 
GCTGATCGTA TGTTGGACAT GGGTTTCGAG CCGCAGATCC GTCGCATCGT CGAACAGCTA 
AACATGCCGC CAACCGGACA GCGCCAGACG CTTATGTTCT CGGCCACATT CCCCAAGCAG 
ATCCAGGAGT TAGCCAGCGA TTTCCTCAGC AACTACATTT TCTTGGCCGT TGGTCGTGTG 
GGCTCCACAT CTGAGAACAT TACGCAGACA ATCCTGTGGG TCTATGAACC CGACAAGCGC 
TCGTATCTGC TGGATCTGCT CTCGTCTATC CGCGATGGCC CCGAATACAC CAAAGACAGC 
CTAACACTGA TCTTTGTGGA GACTAAGAAG GGTGCAGACT CGCTGGAGGA GTTCCTGTAC 
CAGTGCAATC ATCCCGTCAC TAGCATCCAC GGTGATCGCA CGCAGAAGGA GCGCGAGGAG 
GCACTGCGCT GCTTCCGATC GGGCGACTGC CCCATCCTGG TGGCCACAGC CGTGGCCGCT 
CGTGGCCTGG ACATTCCGCA CGTTAAGCAC GTTATTAACT TTGACCTGCC CTCGGATGTG 
GAGGAGTATG TCCACCGTAT CGGGCGTACC GGACGTATGG GTAATCTTGG TGTGGCCACC 
TCCTTTTTCA ACGAAAAGAA CCGCAACATT TGCTCCGATC TCTTGGAGCT GCTGATCGAA 
ACGAAGCAGG AGATCCCCAG TTTCATGGAG GATATGAGTT CGGATCGCGG ACATGGCGGA 
GCCAAGCGGG CTGGACGAGG CGGTGGTGGA CGTTATGGTG GCGGCTTCGG CTCCAGGGAT 
TACCGTCAAA GCTCTGGCGG CGGCGGTGGC GGACGCAGTG GACCGCCACC ACGTAGCGGT 
GGTTCCGGAT CAGGAGGCGG CGGCGGCAGT TACCGCAGCA ATGGAAACTC GTACGGTAAG 
TTTGGCGGCA ATTCGGGCGG CGGTGGATAC TATGGCGGCG GCGCCGGTGG TGGCTCCTAT 
GGCGGCTCCT ACGGCGGCGG CAGTGCCTCG CACTCTTCGA ACGCACCCGA CTGGTGGGCT 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bel cDNA ORF clone, Drosophila melanogaster(fruit fly)

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