ND-SGDH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ND-SGDH
Entrez Gene ID	46260
Full Name	NADH dehydrogenase (ubiquinone) SGDH subunit
Synonyms	CG9762,Dmel\CG9762,NISM,l(3)neo18
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) SGDH subunit Names CG9762 gene product from transcript CG9762-RA CG9762-PA CG9762-PB NADH ubiquinone oxidoreductase SGDH NADH-ubiquinone oxidoreductase SGDH subunit NADH:ubiquinone oxidoreductase SGDH subunit ND-SGDH-PA ND-SGDH-PB lethal (3) neo18
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68F5-68F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274813.1	NP_001261742.1	NADH dehydrogenase (ubiquinone) SGDH subunit, isoform B
NM_143785.3	NP_652042.1	NADH dehydrogenase (ubiquinone) SGDH subunit, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NDUFB5	NP_788829.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007297	XP_308524.4
Homo sapiens (human)	NDUFB5	NP_002483.1
Danio rerio (zebrafish)	ndufb5	NP_001099066.1
Canis lupus familiaris (dog)	NDUFB5	XP_535812.1
Canis lupus familiaris (dog)	LOC102154590	XP_005615552.1
	NDUFB5	XP_001107947.1
Drosophila melanogaster (fruit fly)	l(3)neo18	NP_001261742.1
Pan troglodytes (chimpanzee)	NDUFB5	NP_001065274.1
Mus musculus (house mouse)	Ndufb5	NP_079592.2
Rattus norvegicus (Norway rat)	Ndufb5	NP_001099896.1
Gallus gallus (chicken)	NDUFB5	NP_001006553.1
Xenopus tropicalis (tropical clawed frog)	ndufb5	NP_001017303.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ND-SGDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ND-SGDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19443	NM_001274813.1 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) SGDH subunit (ND-SGDH), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19443	NM_143785.3 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) SGDH subunit (ND-SGDH), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ND-SGDH ( NM_001274813.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261742.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) SGDH subunit (ND-SGDH), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19443
Clone ID Related Accession (Same CDS sequence)	NM_001274813.1 , NM_143785.3
Accession Version	NM_001274813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) SGDH subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGGTT GGAGCCGTTT GCTGTCGCCG GCGGCGAAGT TCGCCAGCTA CCGGGCTGTC 
CTGCAAGAGC CCGCGTGCCG GAATGCCCTG CACCAACAGC TGCGCAGAAT GGGAGGCGAT 
CATGGACACC ACCAAATGAT CATCAAGCCA TCCCGCTTCC AGTGGGACAA GTTCAAGGAT 
CTGTTGCACT TTTACGTAAT GCTCGGCGTC ATCCCAGTCA CCGCATTGGT TCTCTACGCG 
AACATCTTTG TGGGACCCGC GCAGCTCGCC GAGATTCCCG AGGGCTATGA GCCCAAGCAC 
TGGGAGTACG AGAAGCACCC CATCTCGCGC TTTATTTCCC GCTACATTTT GAACTCGGAT 
CAGCAAAACT ACGAGAAATC CCTGCACTAT CTCTACGAGG AGAACGAAAA AGCCCAGATT 
CGACTCCTTG AGGACGAAGT ACGTCGCAAG ATGTCGGAGC GCAATGATTA CCAGGCCTAC 
TACTACCGAC CATCGGTGGC CAAGTACCAC AGGATTTCGA AAGAGGCTGC TGACGAGCTG 
GAGGCTCTGC GCGGAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261742.1
CDS	73..633
Translation	MVGWSRLLSPAAKFASYRAVLQEPACRNALHQQLRRMGGDHGHHQMIIKPSRFQWDKFKDLLHFYVMLGVIPVTALVLYANIFVGPAQLAEIPEGYEPKHWEYEKHPISRFISRYILNSDQQNYEKSLHYLYEENEKAQIRLLEDEVRRKMSERNDYQAYYYRPSVAKYHRISKEAADELEALRGD

Target ORF information:

RefSeq Version	NM_001274813.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) SGDH subunit (ND-SGDH), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274813.1

ORF Insert Sequence:

ATGGTCGGTT GGAGCCGTTT GCTGTCGCCG GCGGCGAAGT TCGCCAGCTA CCGGGCTGTC 
CTGCAAGAGC CCGCGTGCCG GAATGCCCTG CACCAACAGC TGCGCAGAAT GGGAGGCGAT 
CATGGACACC ACCAAATGAT CATCAAGCCA TCCCGCTTCC AGTGGGACAA GTTCAAGGAT 
CTGTTGCACT TTTACGTAAT GCTCGGCGTC ATCCCAGTCA CCGCATTGGT TCTCTACGCG 
AACATCTTTG TGGGACCCGC GCAGCTCGCC GAGATTCCCG AGGGCTATGA GCCCAAGCAC 
TGGGAGTACG AGAAGCACCC CATCTCGCGC TTTATTTCCC GCTACATTTT GAACTCGGAT 
CAGCAAAACT ACGAGAAATC CCTGCACTAT CTCTACGAGG AGAACGAAAA AGCCCAGATT 
CGACTCCTTG AGGACGAAGT ACGTCGCAAG ATGTCGGAGC GCAATGATTA CCAGGCCTAC 
TACTACCGAC CATCGGTGGC CAAGTACCAC AGGATTTCGA AAGAGGCTGC TGACGAGCTG 
GAGGCTCTGC GCGGAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ND-SGDH ( NM_143785.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652042.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) SGDH subunit (ND-SGDH), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19443
Clone ID Related Accession (Same CDS sequence)	NM_001274813.1 , NM_143785.3
Accession Version	NM_143785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) SGDH subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_143785.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGGTT GGAGCCGTTT GCTGTCGCCG GCGGCGAAGT TCGCCAGCTA CCGGGCTGTC 
CTGCAAGAGC CCGCGTGCCG GAATGCCCTG CACCAACAGC TGCGCAGAAT GGGAGGCGAT 
CATGGACACC ACCAAATGAT CATCAAGCCA TCCCGCTTCC AGTGGGACAA GTTCAAGGAT 
CTGTTGCACT TTTACGTAAT GCTCGGCGTC ATCCCAGTCA CCGCATTGGT TCTCTACGCG 
AACATCTTTG TGGGACCCGC GCAGCTCGCC GAGATTCCCG AGGGCTATGA GCCCAAGCAC 
TGGGAGTACG AGAAGCACCC CATCTCGCGC TTTATTTCCC GCTACATTTT GAACTCGGAT 
CAGCAAAACT ACGAGAAATC CCTGCACTAT CTCTACGAGG AGAACGAAAA AGCCCAGATT 
CGACTCCTTG AGGACGAAGT ACGTCGCAAG ATGTCGGAGC GCAATGATTA CCAGGCCTAC 
TACTACCGAC CATCGGTGGC CAAGTACCAC AGGATTTCGA AAGAGGCTGC TGACGAGCTG 
GAGGCTCTGC GCGGAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652042.1
CDS	184..744
Translation	MVGWSRLLSPAAKFASYRAVLQEPACRNALHQQLRRMGGDHGHHQMIIKPSRFQWDKFKDLLHFYVMLGVIPVTALVLYANIFVGPAQLAEIPEGYEPKHWEYEKHPISRFISRYILNSDQQNYEKSLHYLYEENEKAQIRLLEDEVRRKMSERNDYQAYYYRPSVAKYHRISKEAADELEALRGD

Target ORF information:

RefSeq Version	NM_143785.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) SGDH subunit (ND-SGDH), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143785.3

ORF Insert Sequence:

ATGGTCGGTT GGAGCCGTTT GCTGTCGCCG GCGGCGAAGT TCGCCAGCTA CCGGGCTGTC 
CTGCAAGAGC CCGCGTGCCG GAATGCCCTG CACCAACAGC TGCGCAGAAT GGGAGGCGAT 
CATGGACACC ACCAAATGAT CATCAAGCCA TCCCGCTTCC AGTGGGACAA GTTCAAGGAT 
CTGTTGCACT TTTACGTAAT GCTCGGCGTC ATCCCAGTCA CCGCATTGGT TCTCTACGCG 
AACATCTTTG TGGGACCCGC GCAGCTCGCC GAGATTCCCG AGGGCTATGA GCCCAAGCAC 
TGGGAGTACG AGAAGCACCC CATCTCGCGC TTTATTTCCC GCTACATTTT GAACTCGGAT 
CAGCAAAACT ACGAGAAATC CCTGCACTAT CTCTACGAGG AGAACGAAAA AGCCCAGATT 
CGACTCCTTG AGGACGAAGT ACGTCGCAAG ATGTCGGAGC GCAATGATTA CCAGGCCTAC 
TACTACCGAC CATCGGTGGC CAAGTACCAC AGGATTTCGA AAGAGGCTGC TGACGAGCTG 
GAGGCTCTGC GCGGAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ND-SGDH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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