heph cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following heph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the heph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa06931 NM_001170320.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant S, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06931 NM_001170321.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant T, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06931 NM_001170322.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant U, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06931 NM_079964.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_001170323.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant V, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_176597.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_176596.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_170581.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_170582.4
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_176601.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_176602.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_176598.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_001104524.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_176600.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06935 NM_001170317.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06936 NM_001170318.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant Q, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa08901 NM_001104522.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa08902 NM_176599.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa08903 NM_001104523.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa22265 NM_001300701.1
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant Z, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa06932 NM_001170316.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa06932 NM_176603.3
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa06932 NM_001170319.1
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant R, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa06933 NM_001260469.2
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant W, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa06934 NM_001260470.1
Latest version!
Drosophila melanogaster hephaestus (heph), transcript variant Y, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa06931
    Clone ID Related Accession (Same CDS sequence) NM_079964.3 , NM_001170321.2 , NM_001170320.2 , NM_001170322.2
    Accession Version NM_079964.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_079964.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524703.1
    CDS399..2144
    Translation

    Target ORF information:

    RefSeq Version NM_079964.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079964.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06931
    Clone ID Related Accession (Same CDS sequence) NM_079964.3 , NM_001170321.2 , NM_001170320.2 , NM_001170322.2
    Accession Version NM_001170321.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform T
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170321.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163792.1
    CDS577..2322
    Translation

    Target ORF information:

    RefSeq Version NM_001170321.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant T, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06931
    Clone ID Related Accession (Same CDS sequence) NM_079964.3 , NM_001170321.2 , NM_001170320.2 , NM_001170322.2
    Accession Version NM_001170320.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170320.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163791.1
    CDS1135..2880
    Translation

    Target ORF information:

    RefSeq Version NM_001170320.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant S, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170320.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06931
    Clone ID Related Accession (Same CDS sequence) NM_079964.3 , NM_001170321.2 , NM_001170320.2 , NM_001170322.2
    Accession Version NM_001170322.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform U
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170322.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163793.1
    CDS1157..2902
    Translation

    Target ORF information:

    RefSeq Version NM_001170322.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant U, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170322.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGCGGAA GCGACGAACT TTTGAGTCAA GCAGCGGTCA TGGCGCCCGC TTCCGACAAT 
    AACAATCAGG ACCTGGCCAC AAAGAAGGCC AAACTGGAGC CGGGCACTGT GCTGGCCGGC
    GGAATTGCCA AGGCCTCAAA AGTCATCCAC TTGCGCAACA TTCCGAACGA GTCCGGCGAG
    GCAGATGTGA TTGCCCTGGG CATTCCGTTT GGACGTGTGA CCAACGTGCT GGTGCTCAAG
    GGCAAGAACC AGGCTTTCAT CGAGATGGCC GACGAGATCT CCGCAACGTC AATGGTGTCC
    TGTTACACAG TAACTCCGCC CCAGATGCGC GGCCGCATGG TCTACGTGCA GTTTTCTAAT
    CATCGCGAAC TAAAGACGGA CCAAGGTCAC AACAACTCGA CCGCCCATAG CGACTACAGC
    GTCCAGTCCC CGGCGAGCGG ATCGCCTCTG CCCCTTAGCG CCGCTGCCAA CGCGACCAGC
    AACAATGCAA ACAGCTCAAG CGACAGCAAC AGCGCCATGG GAATCTTACA AAACACAAGC
    GCCGTCAATG CGGGCGGCAA CACCAACGCT GCCGGGGGAC CCAATACTGT GCTCCGTGTG
    ATTGTCGAGT CCTTGATGTA CCCCGTCTCG CTGGACATAC TGCACCAGAT CTTCCAGCGC
    TACGGCAAGG TGCTGAAGAT TGTCACCTTC ACAAAGAACA ATTCATTCCA GGCCCTCATC
    CAATATCCGG ACGCCAATTC CGCCCAGCAC GCCAAGTCGC TCCTGGACGG GCAGAATATA
    TACAACGGTT GCTGCACGTT GCGTATTGAT AACAGCAAGC TGACGGCTCT GAATGTTAAG
    TACAACAACG ACAAATCGCG CGACTTCACT AACCCCGCTC TGCCACCTGG TGAACCGGGA
    GTGGATATCA TGCCCACCGC CGGCGGACTG ATGAACACCA ATGATTTGCT TTTGATCGCC
    GCCCGTCAGC GACCTTCTCT CTCAGGTGAT AAAATAGTCA ACGGCCTGGG CGCCCCCGGA
    GTCCTGCCGC CCTTTGCCTT GGGCCTGGGC ACTCCACTGA CCGGTGGATA CAACAACGCA
    CTGCCCAACT TAGCAGCTTT CTCGCTGGCC AACAGTGGCG CCCTGCAGAC GACCGCTCCT
    GCAATGCGAG GTTACTCGAA TGTCTTGCTT GTCTCGAATT TAAATGAGGA GATGGTCACG
    CCTGATGCTC TCTTTACCCT TTTTGGTGTA TACGGCGATG TGCAACGTGT AAAGATTCTG
    TACAACAAGA AGGACTCGGC ACTCATACAA ATGGCGGAGC CCCAGCAAGC TTATTTGGCC
    ATGTCTCACC TTGATAAATT ACGTTTATGG GGAAAGCCGA TACGTGTGAT GGCAAGCAAA
    CACCAGGCCG TCCAGCTGCC CAAGGAGGGA CAGCCGGACG CAGGACTCAC ACGTGACTAC
    TCGCAAAATC CGTTGCACCG CTTCAAGAAA CCGGGAAGCA AGAACTACCA GAACATCTAT
    CCGCCGTCGG CGACACTACA TTTAAGCAAC ATTCCCTCAT CCTGCTCTGA GGATGACATC
    AAAGAAGCTT TCACCTCAAA CAGCTTTGAA GTTAAGGCAT TCAAATTTTT CCCGAAGGAC
    CGTAAAATGG CACTGCTTCA ACTGTTGTCC GTGGAGGAGG CTGTCCTAGC ACTGATTAAG
    ATGCACAACC ACCAGCTGTC CGAGTCGAAT CACCTGCGTG TAAGCTTCTC GAAGTCGAAC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_176596.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176596.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788773.1
    CDS254..2080
    Translation

    Target ORF information:

    RefSeq Version NM_176596.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176596.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_170581.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170581.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733460.1
    CDS208..2034
    Translation

    Target ORF information:

    RefSeq Version NM_170581.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170581.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_001104524.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104524.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097994.1
    CDS672..2498
    Translation

    Target ORF information:

    RefSeq Version NM_001104524.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104524.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_170582.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170582.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733461.2
    CDS672..2498
    Translation

    Target ORF information:

    RefSeq Version NM_170582.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170582.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_176600.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176600.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788777.1
    CDS365..2191
    Translation

    Target ORF information:

    RefSeq Version NM_176600.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176600.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_001170323.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform V
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170323.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163794.1
    CDS931..2757
    Translation

    Target ORF information:

    RefSeq Version NM_001170323.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant V, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_176598.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_176598.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788775.1
    CDS766..2592
    Translation

    Target ORF information:

    RefSeq Version NM_176598.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176598.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_176602.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176602.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788779.1
    CDS185..2011
    Translation

    Target ORF information:

    RefSeq Version NM_176602.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176602.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_176597.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176597.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788774.1
    CDS1160..2986
    Translation

    Target ORF information:

    RefSeq Version NM_176597.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176597.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_176601.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176601.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788778.1
    CDS423..2249
    Translation

    Target ORF information:

    RefSeq Version NM_176601.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176601.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06935
    Clone ID Related Accession (Same CDS sequence) NM_176596.3 , NM_170581.2 , NM_001104524.3 , NM_170582.4 , NM_176600.3 , NM_001170323.2 , NM_176598.2 , NM_176602.3 , NM_176597.3 , NM_176601.3 , NM_001170317.2
    Accession Version NM_001170317.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170317.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163788.1
    CDS395..2221
    Translation

    Target ORF information:

    RefSeq Version NM_001170317.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06936
    Clone ID Related Accession (Same CDS sequence) NM_001170318.2
    Accession Version NM_001170318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform Q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170318.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGACG AGATCTCCGC AACGTCAATG GTGTCCTGTT ACACAGTAAC TCCGCCCCAG 
    ATGCGCGGCC GCATGGTCTA CGTGCAGTTT TCTAATCATC GCGAACTAAA GACGGACCAA
    GGTCACAACA ACTCGACCGC CCATAGCGAC TACAGCGTCC AGTCCCCGGC GAGCGGATCG
    CCTCTGCCCC TTAGCGCCGC TGCCAACGCG ACCAGCAACA ATGCAAACAG CTCAAGCGAC
    AGCAACAGCG CCATGGGAAT CTTACAAAAC ACAAGCGCCG TCAATGCGGG CGGCAACACC
    AACGCTGCCG GGGGACCCAA TACTGTGCTC CGTGTGATTG TCGAGTCCTT GATGTACCCC
    GTCTCGCTGG ACATACTGCA CCAGATCTTC CAGCGCTACG GCAAGGTGCT GAAGATTGTC
    ACCTTCACAA AGAACAATTC ATTCCAGGCC CTCATCCAAT ATCCGGACGC CAATTCCGCC
    CAGCACGCCA AGTCGCTCCT GGACGGGCAG AATATATACA ACGGTTGCTG CACGTTGCGT
    ATTGATAACA GCAAGCTGAC GGCTCTGAAT GTTAAGTACA ACAACGACAA ATCGCGCGAC
    TTCACTAACC CCGCTCTGCC ACCTGGTGAA CCGGGAGTGG ATATCATGCC CACCGCCGGC
    GGACTGATGA ACACCAATGA TTTGCTTTTG ATCGCCGCCC GTCAGCGACC TTCTCTCTCA
    GGTGATAAAA TAGTCAACGG CCTGGGCGCC CCCGGAGTCC TGCCGCCCTT TGCCTTGGGC
    CTGGGCACTC CACTGACCGG TGGATACAAC AACGCACTGC CCAACTTAGC AGCTTTCTCG
    CTGGCCAACA GTGGCGCCCT GCAGACGACC GCTCCTGCAA TGCGAGGTTA CTCGAATGTC
    TTGCTTGTCT CGAATTTAAA TGAGGAGATG GTCACGCCTG ATGCTCTCTT TACCCTTTTT
    GGTGTATACG GCGATGTGCA ACGTGTAAAG ATTCTGTACA ACAAGAAGGA CTCGGCACTC
    ATACAAATGG CGGAGCCCCA GCAAGCTTAT TTGGCCATGT CTCACCTTGA TAAATTACGT
    TTATGGGGAA AGCCGATACG TGTGATGGCA AGCAAACACC AGGCCGTCCA GCTGCCCAAG
    GAGGGACAGC CGGACGCAGG ACTCACACGT GACTACTCGC AAAATCCGTT GCACCGCTTC
    AAGAAACCGG GAAGCAAGAA CTACCAGAAC ATCTATCCGC CGTCGGCGAC ACTACATTTA
    AGCAACATTC CCTCATCCTG CTCTGAGGAT GACATCAAAG AAGCTTTCAC CTCAAACAGC
    TTTGAAGTTA AGGCATTCAA ATTTTTCCCG AAGGACCGTA AAATGGCACT GCTTCAACTG
    TTGTCCGTGG AGGAGGCTGT CCTAGCACTG ATTAAGATGC ACAACCACCA GCTGTCCGAG
    TCGAATCACC TGCGTGTAAG CTTCTCGAAG TCGAACATCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163789.1
    CDS539..2020
    Translation

    Target ORF information:

    RefSeq Version NM_001170318.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant Q, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCCGACG AGATCTCCGC AACGTCAATG GTGTCCTGTT ACACAGTAAC TCCGCCCCAG 
    ATGCGCGGCC GCATGGTCTA CGTGCAGTTT TCTAATCATC GCGAACTAAA GACGGACCAA
    GGTCACAACA ACTCGACCGC CCATAGCGAC TACAGCGTCC AGTCCCCGGC GAGCGGATCG
    CCTCTGCCCC TTAGCGCCGC TGCCAACGCG ACCAGCAACA ATGCAAACAG CTCAAGCGAC
    AGCAACAGCG CCATGGGAAT CTTACAAAAC ACAAGCGCCG TCAATGCGGG CGGCAACACC
    AACGCTGCCG GGGGACCCAA TACTGTGCTC CGTGTGATTG TCGAGTCCTT GATGTACCCC
    GTCTCGCTGG ACATACTGCA CCAGATCTTC CAGCGCTACG GCAAGGTGCT GAAGATTGTC
    ACCTTCACAA AGAACAATTC ATTCCAGGCC CTCATCCAAT ATCCGGACGC CAATTCCGCC
    CAGCACGCCA AGTCGCTCCT GGACGGGCAG AATATATACA ACGGTTGCTG CACGTTGCGT
    ATTGATAACA GCAAGCTGAC GGCTCTGAAT GTTAAGTACA ACAACGACAA ATCGCGCGAC
    TTCACTAACC CCGCTCTGCC ACCTGGTGAA CCGGGAGTGG ATATCATGCC CACCGCCGGC
    GGACTGATGA ACACCAATGA TTTGCTTTTG ATCGCCGCCC GTCAGCGACC TTCTCTCTCA
    GGTGATAAAA TAGTCAACGG CCTGGGCGCC CCCGGAGTCC TGCCGCCCTT TGCCTTGGGC
    CTGGGCACTC CACTGACCGG TGGATACAAC AACGCACTGC CCAACTTAGC AGCTTTCTCG
    CTGGCCAACA GTGGCGCCCT GCAGACGACC GCTCCTGCAA TGCGAGGTTA CTCGAATGTC
    TTGCTTGTCT CGAATTTAAA TGAGGAGATG GTCACGCCTG ATGCTCTCTT TACCCTTTTT
    GGTGTATACG GCGATGTGCA ACGTGTAAAG ATTCTGTACA ACAAGAAGGA CTCGGCACTC
    ATACAAATGG CGGAGCCCCA GCAAGCTTAT TTGGCCATGT CTCACCTTGA TAAATTACGT
    TTATGGGGAA AGCCGATACG TGTGATGGCA AGCAAACACC AGGCCGTCCA GCTGCCCAAG
    GAGGGACAGC CGGACGCAGG ACTCACACGT GACTACTCGC AAAATCCGTT GCACCGCTTC
    AAGAAACCGG GAAGCAAGAA CTACCAGAAC ATCTATCCGC CGTCGGCGAC ACTACATTTA
    AGCAACATTC CCTCATCCTG CTCTGAGGAT GACATCAAAG AAGCTTTCAC CTCAAACAGC
    TTTGAAGTTA AGGCATTCAA ATTTTTCCCG AAGGACCGTA AAATGGCACT GCTTCAACTG
    TTGTCCGTGG AGGAGGCTGT CCTAGCACTG ATTAAGATGC ACAACCACCA GCTGTCCGAG
    TCGAATCACC TGCGTGTAAG CTTCTCGAAG TCGAACATCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08901
    Clone ID Related Accession (Same CDS sequence) NM_001104522.3
    Accession Version NM_001104522.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104522.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGGTAAGG ATCGACGAAC AATATATATA TAAACTGAAC
    TATTTGCGCG GAAGCGACGA ACTTTTGAGT CAAGCAGCGG TCATGGCGCC CGCTTCCGAC
    AATAACAATC AGGACCTGGC CACAAAGAAG GCCAAACTGG AGCCGGGCAC TGTGCTGGCC
    GGCGGAATTG CCAAGGCCTC AAAAGTCATC CACTTGCGCA ACATTCCGAA CGAGTCCGGC
    GAGGCAGATG TGATTGCCCT GGGCATTCCG TTTGGACGTG TGACCAACGT GCTGGTGCTC
    AAGGGCAAGA ACCAGGCTTT CATCGAGATG GCCGACGAGA TCTCCGCAAC GTCAATGGTG
    TCCTGTTACA CAGTAACTCC GCCCCAGATG CGCGGCCGCA TGGTCTACGT GCAGTTTTCT
    AATCATCGCG AACTAAAGAC GGACCAAGGT CACAACAACT CGACCGCCCA TAGCGACTAC
    AGCGTCCAGT CCCCGGCGAG CGGATCGCCT CTGCCCCTTA GCGCCGCTGC CAACGCGACC
    AGCAACAATG CAAACAGCTC AAGCGACAGC AACAGCGCCA TGGGAATCTT ACAAAACACA
    AGCGCCGTCA ATGCGGGCGG CAACACCAAC GCTGCCGGGG GACCCAATAC TGTGCTCCGT
    GTGATTGTCG AGTCCTTGAT GTACCCCGTC TCGCTGGACA TACTGCACCA GATCTTCCAG
    CGCTACGGCA AGGTGCTGAA GATTGTCACC TTCACAAAGA ACAATTCATT CCAGGCCCTC
    ATCCAATATC CGGACGCCAA TTCCGCCCAG CACGCCAAGT CGCTCCTGGA CGGGCAGAAT
    ATATACAACG GTTGCTGCAC GTTGCGTATT GATAACAGCA AGCTGACGGC TCTGAATGTT
    AAGTACAACA ACGACAAATC GCGCGACTTC ACTAACCCCG CTCTGCCACC TGGTGAACCG
    GGAGTGGATA TCATGCCCAC CGCCGGCGGA CTGATGAACA CCAATGATTT GCTTTTGATC
    GCCGCCCGTC AGCGACCTTC TCTCTCAGGT GATAAAATAG TCAACGGCCT GGGCGCCCCC
    GGAGTCCTGC CGCCCTTTGC CTTGGGCCTG GGCACTCCAC TGACCGGTGG ATACAACAAC
    GCACTGCCCA ACTTAGCAGC TTTCTCGCTG GCCAACAGTG GCGCCCTGCA GACGACCGCT
    CCTGCAATGC GAGGTTACTC GAATGTCTTG CTTGTCTCGA ATTTAAATGA GGAGATGGTC
    ACGCCTGATG CTCTCTTTAC CCTTTTTGGT GTATACGGCG ATGTGCAACG TGTAAAGATT
    CTGTACAACA AGAAGGACTC GGCACTCATA CAAATGGCGG AGCCCCAGCA AGCTTATTTG
    GCCATGTCTC ACCTTGATAA ATTACGTTTA TGGGGAAAGC CGATACGTGT GATGGCAAGC
    AAACACCAGG CCGTCCAGCT GCCCAAGGAG GGACAGCCGG ACGCAGGACT CACACGTGAC
    TACTCGCAAA ATCCGTTGCA CCGCTTCAAG AAACCGGGAA GCAAGAACTA CCAGAACATC
    TATCCGCCGT CGGCGACACT ACATTTAAGC AACATTCCCT CATCCTGCTC TGAGGATGAC
    ATCAAAGAAG CTTTCACCTC AAACAGCTTT GAAGTTAAGG CATTCAAATT TTTCCCGAAG
    GACCGTAAAA TGGCACTGCT TCAACTGTTG TCCGTGGAGG AGGCTGTCCT AGCACTGATT
    AAGATGCACA ACCACCAGCT GTCCGAGTCG AATCACCTGC GTGTAAGCTT CTCGAAGTCG
    AACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097992.1
    CDS365..2233
    Translation

    Target ORF information:

    RefSeq Version NM_001104522.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104522.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGGTAAGG ATCGACGAAC AATATATATA TAAACTGAAC
    TATTTGCGCG GAAGCGACGA ACTTTTGAGT CAAGCAGCGG TCATGGCGCC CGCTTCCGAC
    AATAACAATC AGGACCTGGC CACAAAGAAG GCCAAACTGG AGCCGGGCAC TGTGCTGGCC
    GGCGGAATTG CCAAGGCCTC AAAAGTCATC CACTTGCGCA ACATTCCGAA CGAGTCCGGC
    GAGGCAGATG TGATTGCCCT GGGCATTCCG TTTGGACGTG TGACCAACGT GCTGGTGCTC
    AAGGGCAAGA ACCAGGCTTT CATCGAGATG GCCGACGAGA TCTCCGCAAC GTCAATGGTG
    TCCTGTTACA CAGTAACTCC GCCCCAGATG CGCGGCCGCA TGGTCTACGT GCAGTTTTCT
    AATCATCGCG AACTAAAGAC GGACCAAGGT CACAACAACT CGACCGCCCA TAGCGACTAC
    AGCGTCCAGT CCCCGGCGAG CGGATCGCCT CTGCCCCTTA GCGCCGCTGC CAACGCGACC
    AGCAACAATG CAAACAGCTC AAGCGACAGC AACAGCGCCA TGGGAATCTT ACAAAACACA
    AGCGCCGTCA ATGCGGGCGG CAACACCAAC GCTGCCGGGG GACCCAATAC TGTGCTCCGT
    GTGATTGTCG AGTCCTTGAT GTACCCCGTC TCGCTGGACA TACTGCACCA GATCTTCCAG
    CGCTACGGCA AGGTGCTGAA GATTGTCACC TTCACAAAGA ACAATTCATT CCAGGCCCTC
    ATCCAATATC CGGACGCCAA TTCCGCCCAG CACGCCAAGT CGCTCCTGGA CGGGCAGAAT
    ATATACAACG GTTGCTGCAC GTTGCGTATT GATAACAGCA AGCTGACGGC TCTGAATGTT
    AAGTACAACA ACGACAAATC GCGCGACTTC ACTAACCCCG CTCTGCCACC TGGTGAACCG
    GGAGTGGATA TCATGCCCAC CGCCGGCGGA CTGATGAACA CCAATGATTT GCTTTTGATC
    GCCGCCCGTC AGCGACCTTC TCTCTCAGGT GATAAAATAG TCAACGGCCT GGGCGCCCCC
    GGAGTCCTGC CGCCCTTTGC CTTGGGCCTG GGCACTCCAC TGACCGGTGG ATACAACAAC
    GCACTGCCCA ACTTAGCAGC TTTCTCGCTG GCCAACAGTG GCGCCCTGCA GACGACCGCT
    CCTGCAATGC GAGGTTACTC GAATGTCTTG CTTGTCTCGA ATTTAAATGA GGAGATGGTC
    ACGCCTGATG CTCTCTTTAC CCTTTTTGGT GTATACGGCG ATGTGCAACG TGTAAAGATT
    CTGTACAACA AGAAGGACTC GGCACTCATA CAAATGGCGG AGCCCCAGCA AGCTTATTTG
    GCCATGTCTC ACCTTGATAA ATTACGTTTA TGGGGAAAGC CGATACGTGT GATGGCAAGC
    AAACACCAGG CCGTCCAGCT GCCCAAGGAG GGACAGCCGG ACGCAGGACT CACACGTGAC
    TACTCGCAAA ATCCGTTGCA CCGCTTCAAG AAACCGGGAA GCAAGAACTA CCAGAACATC
    TATCCGCCGT CGGCGACACT ACATTTAAGC AACATTCCCT CATCCTGCTC TGAGGATGAC
    ATCAAAGAAG CTTTCACCTC AAACAGCTTT GAAGTTAAGG CATTCAAATT TTTCCCGAAG
    GACCGTAAAA TGGCACTGCT TCAACTGTTG TCCGTGGAGG AGGCTGTCCT AGCACTGATT
    AAGATGCACA ACCACCAGCT GTCCGAGTCG AATCACCTGC GTGTAAGCTT CTCGAAGTCG
    AACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08902
    Clone ID Related Accession (Same CDS sequence) NM_176599.2
    Accession Version NM_176599.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_176599.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGAGAAT TTGTAGCGAC CATGATGTCC TGCCCCATTC CCATGCCAAT GCCAATTTCT 
    TCATTACACA AATCAGAATT TGAACCGGTT AATATTGGAG TTAAGCGCGG AAGCGACGAA
    CTTTTGAGTC AAGCAGCGGT CATGGCGCCC GCTTCCGACA ATAACAATCA GGACCTGGCC
    ACAAAGAAGG CCAAACTGGA GCCGGGCACT GTGCTGGCCG GCGGAATTGC CAAGGCCTCA
    AAAGTCATCC ACTTGCGCAA CATTCCGAAC GAGTCCGGCG AGGCAGATGT GATTGCCCTG
    GGCATTCCGT TTGGACGTGT GACCAACGTG CTGGTGCTCA AGGGCAAGAA CCAGGCTTTC
    ATCGAGATGG CCGACGAGAT CTCCGCAACG TCAATGGTGT CCTGTTACAC AGTAACTCCG
    CCCCAGATGC GCGGCCGCAT GGTCTACGTG CAGTTTTCTA ATCATCGCGA ACTAAAGACG
    GACCAAGGTC ACAACAACTC GACCGCCCAT AGCGACTACA GCGTCCAGTC CCCGGCGAGC
    GGATCGCCTC TGCCCCTTAG CGCCGCTGCC AACGCGACCA GCAACAATGC AAACAGCTCA
    AGCGACAGCA ACAGCGCCAT GGGAATCTTA CAAAACACAA GCGCCGTCAA TGCGGGCGGC
    AACACCAACG CTGCCGGGGG ACCCAATACT GTGCTCCGTG TGATTGTCGA GTCCTTGATG
    TACCCCGTCT CGCTGGACAT ACTGCACCAG ATCTTCCAGC GCTACGGCAA GGTGCTGAAG
    ATTGTCACCT TCACAAAGAA CAATTCATTC CAGGCCCTCA TCCAATATCC GGACGCCAAT
    TCCGCCCAGC ACGCCAAGTC GCTCCTGGAC GGGCAGAATA TATACAACGG TTGCTGCACG
    TTGCGTATTG ATAACAGCAA GCTGACGGCT CTGAATGTTA AGTACAACAA CGACAAATCG
    CGCGACTTCA CTAACCCCGC TCTGCCACCT GGTGAACCGG GAGTGGATAT CATGCCCACC
    GCCGGCGGAC TGATGAACAC CAATGATTTG CTTTTGATCG CCGCCCGTCA GCGACCTTCT
    CTCTCAGGTG ATAAAATAGT CAACGGCCTG GGCGCCCCCG GAGTCCTGCC GCCCTTTGCC
    TTGGGCCTGG GCACTCCACT GACCGGTGGA TACAACAACG CACTGCCCAA CTTAGCAGCT
    TTCTCGCTGG CCAACAGTGG CGCCCTGCAG ACGACCGCTC CTGCAATGCG AGGTTACTCG
    AATGTCTTGC TTGTCTCGAA TTTAAATGAG GAGATGGTCA CGCCTGATGC TCTCTTTACC
    CTTTTTGGTG TATACGGCGA TGTGCAACGT GTAAAGATTC TGTACAACAA GAAGGACTCG
    GCACTCATAC AAATGGCGGA GCCCCAGCAA GCTTATTTGG CCATGTCTCA CCTTGATAAA
    TTACGTTTAT GGGGAAAGCC GATACGTGTG ATGGCAAGCA AACACCAGGC CGTCCAGCTG
    CCCAAGGAGG GACAGCCGGA CGCAGGACTC ACACGTGACT ACTCGCAAAA TCCGTTGCAC
    CGCTTCAAGA AACCGGGAAG CAAGAACTAC CAGAACATCT ATCCGCCGTC GGCGACACTA
    CATTTAAGCA ACATTCCCTC ATCCTGCTCT GAGGATGACA TCAAAGAAGC TTTCACCTCA
    AACAGCTTTG AAGTTAAGGC ATTCAAATTT TTCCCGAAGG ACCGTAAAAT GGCACTGCTT
    CAACTGTTGT CCGTGGAGGA GGCTGTCCTA GCACTGATTA AGATGCACAA CCACCAGCTG
    TCCGAGTCGA ATCACCTGCG TGTAAGCTTC TCGAAGTCGA ACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788776.2
    CDS304..2151
    Translation

    Target ORF information:

    RefSeq Version NM_176599.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176599.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGAGAAT TTGTAGCGAC CATGATGTCC TGCCCCATTC CCATGCCAAT GCCAATTTCT 
    TCATTACACA AATCAGAATT TGAACCGGTT AATATTGGAG TTAAGCGCGG AAGCGACGAA
    CTTTTGAGTC AAGCAGCGGT CATGGCGCCC GCTTCCGACA ATAACAATCA GGACCTGGCC
    ACAAAGAAGG CCAAACTGGA GCCGGGCACT GTGCTGGCCG GCGGAATTGC CAAGGCCTCA
    AAAGTCATCC ACTTGCGCAA CATTCCGAAC GAGTCCGGCG AGGCAGATGT GATTGCCCTG
    GGCATTCCGT TTGGACGTGT GACCAACGTG CTGGTGCTCA AGGGCAAGAA CCAGGCTTTC
    ATCGAGATGG CCGACGAGAT CTCCGCAACG TCAATGGTGT CCTGTTACAC AGTAACTCCG
    CCCCAGATGC GCGGCCGCAT GGTCTACGTG CAGTTTTCTA ATCATCGCGA ACTAAAGACG
    GACCAAGGTC ACAACAACTC GACCGCCCAT AGCGACTACA GCGTCCAGTC CCCGGCGAGC
    GGATCGCCTC TGCCCCTTAG CGCCGCTGCC AACGCGACCA GCAACAATGC AAACAGCTCA
    AGCGACAGCA ACAGCGCCAT GGGAATCTTA CAAAACACAA GCGCCGTCAA TGCGGGCGGC
    AACACCAACG CTGCCGGGGG ACCCAATACT GTGCTCCGTG TGATTGTCGA GTCCTTGATG
    TACCCCGTCT CGCTGGACAT ACTGCACCAG ATCTTCCAGC GCTACGGCAA GGTGCTGAAG
    ATTGTCACCT TCACAAAGAA CAATTCATTC CAGGCCCTCA TCCAATATCC GGACGCCAAT
    TCCGCCCAGC ACGCCAAGTC GCTCCTGGAC GGGCAGAATA TATACAACGG TTGCTGCACG
    TTGCGTATTG ATAACAGCAA GCTGACGGCT CTGAATGTTA AGTACAACAA CGACAAATCG
    CGCGACTTCA CTAACCCCGC TCTGCCACCT GGTGAACCGG GAGTGGATAT CATGCCCACC
    GCCGGCGGAC TGATGAACAC CAATGATTTG CTTTTGATCG CCGCCCGTCA GCGACCTTCT
    CTCTCAGGTG ATAAAATAGT CAACGGCCTG GGCGCCCCCG GAGTCCTGCC GCCCTTTGCC
    TTGGGCCTGG GCACTCCACT GACCGGTGGA TACAACAACG CACTGCCCAA CTTAGCAGCT
    TTCTCGCTGG CCAACAGTGG CGCCCTGCAG ACGACCGCTC CTGCAATGCG AGGTTACTCG
    AATGTCTTGC TTGTCTCGAA TTTAAATGAG GAGATGGTCA CGCCTGATGC TCTCTTTACC
    CTTTTTGGTG TATACGGCGA TGTGCAACGT GTAAAGATTC TGTACAACAA GAAGGACTCG
    GCACTCATAC AAATGGCGGA GCCCCAGCAA GCTTATTTGG CCATGTCTCA CCTTGATAAA
    TTACGTTTAT GGGGAAAGCC GATACGTGTG ATGGCAAGCA AACACCAGGC CGTCCAGCTG
    CCCAAGGAGG GACAGCCGGA CGCAGGACTC ACACGTGACT ACTCGCAAAA TCCGTTGCAC
    CGCTTCAAGA AACCGGGAAG CAAGAACTAC CAGAACATCT ATCCGCCGTC GGCGACACTA
    CATTTAAGCA ACATTCCCTC ATCCTGCTCT GAGGATGACA TCAAAGAAGC TTTCACCTCA
    AACAGCTTTG AAGTTAAGGC ATTCAAATTT TTCCCGAAGG ACCGTAAAAT GGCACTGCTT
    CAACTGTTGT CCGTGGAGGA GGCTGTCCTA GCACTGATTA AGATGCACAA CCACCAGCTG
    TCCGAGTCGA ATCACCTGCG TGTAAGCTTC TCGAAGTCGA ACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08903
    Clone ID Related Accession (Same CDS sequence) NM_001104523.3
    Accession Version NM_001104523.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104523.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG 
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097993.1
    CDS1347..3053
    Translation

    Target ORF information:

    RefSeq Version NM_001104523.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG 
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGGTGA TAAAATAGTC
    AACGGCCTGG GCGCCCCCGG AGTCCTGCCG CCCTTTGCCT TGGGCCTGGG CACTCCACTG
    ACCGGTGGAT ACAACAACGC ACTGCCCAAC TTAGCAGCTT TCTCGCTGGC CAACAGTGGC
    GCCCTGCAGA CGACCGCTCC TGCAATGCGA GGTTACTCGA ATGTCTTGCT TGTCTCGAAT
    TTAAATGAGG AGATGGTCAC GCCTGATGCT CTCTTTACCC TTTTTGGTGT ATACGGCGAT
    GTGCAACGTG TAAAGATTCT GTACAACAAG AAGGACTCGG CACTCATACA AATGGCGGAG
    CCCCAGCAAG CTTATTTGGC CATGTCTCAC CTTGATAAAT TACGTTTATG GGGAAAGCCG
    ATACGTGTGA TGGCAAGCAA ACACCAGGCC GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC
    GCAGGACTCA CACGTGACTA CTCGCAAAAT CCGTTGCACC GCTTCAAGAA ACCGGGAAGC
    AAGAACTACC AGAACATCTA TCCGCCGTCG GCGACACTAC ATTTAAGCAA CATTCCCTCA
    TCCTGCTCTG AGGATGACAT CAAAGAAGCT TTCACCTCAA ACAGCTTTGA AGTTAAGGCA
    TTCAAATTTT TCCCGAAGGA CCGTAAAATG GCACTGCTTC AACTGTTGTC CGTGGAGGAG
    GCTGTCCTAG CACTGATTAA GATGCACAAC CACCAGCTGT CCGAGTCGAA TCACCTGCGT
    GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22265
    Clone ID Related Accession (Same CDS sequence) NM_001300701.1
    Accession Version NM_001300701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform Z
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGTCAA CGGCCTGGGC
    GCCCCCGGAG TCCTGCCGCC CTTTGCCTTG GGCCTGGGCA CTCCACTGAC CGGTGGATAC
    AACAACGCAC TGCCCAACTT AGCAGCTTTC TCGCTGGCCA ACAGTGGCGC CCTGCAGACG
    ACCGCTCCTG CAATGCGAGG TTACTCGAAT GTCTTGCTTG TCTCGAATTT AAATGAGGAG
    ATGGTCACGC CTGATGCTCT CTTTACCCTT TTTGGTGTAT ACGGCGATGT GCAACGTGTA
    AAGATTCTGT ACAACAAGAA GGACTCGGCA CTCATACAAA TGGCGGAGCC CCAGCAAGCT
    TATTTGGCCA TGTCTCACCT TGATAAATTA CGTTTATGGG GAAAGCCGAT ACGTGTGATG
    GCAAGCAAAC ACCAGGCCGT CCAGCTGCCC AAGGAGGGAC AGCCGGACGC AGGACTCACA
    CGTGACTACT CGCAAAATCC GTTGCACCGC TTCAAGAAAC CGGGAAGCAA GAACTACCAG
    AACATCTATC CGCCGTCGGC GACACTACAT TTAAGCAACA TTCCCTCATC CTGCTCTGAG
    GATGACATCA AAGAAGCTTT CACCTCAAAC AGCTTTGAAG TTAAGGCATT CAAATTTTTC
    CCGAAGGACC GTAAAATGGC ACTGCTTCAA CTGTTGTCCG TGGAGGAGGC TGTCCTAGCA
    CTGATTAAGA TGCACAACCA CCAGCTGTCC GAGTCGAATC ACCTGCGTGT AAGCTTCTCG
    AAGTCGAACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287630.1
    CDS1298..3112
    Translation

    Target ORF information:

    RefSeq Version NM_001300701.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant Z, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCGCGGA AGCGACGAAC TTTTGAGTCA AGCAGCGGTC
    ATGGCGCCCG CTTCCGACAA TAACAATCAG GACCTGGCCA CAAAGAAGGC CAAACTGGAG
    CCGGGCACTG TGCTGGCCGG CGGAATTGCC AAGGCCTCAA AAGTCATCCA CTTGCGCAAC
    ATTCCGAACG AGTCCGGCGA GGCAGATGTG ATTGCCCTGG GCATTCCGTT TGGACGTGTG
    ACCAACGTGC TGGTGCTCAA GGGCAAGAAC CAGGCTTTCA TCGAGATGGC CGACGAGATC
    TCCGCAACGT CAATGGTGTC CTGTTACACA GTAACTCCGC CCCAGATGCG CGGCCGCATG
    GTCTACGTGC AGTTTTCTAA TCATCGCGAA CTAAAGACGG ACCAAGGTCA CAACAACTCG
    ACCGCCCATA GCGACTACAG CGTCCAGTCC CCGGCGAGCG GATCGCCTCT GCCCCTTAGC
    GCCGCTGCCA ACGCGACCAG CAACAATGCA AACAGCTCAA GCGACAGCAA CAGCGCCATG
    GGAATCTTAC AAAACACAAG CGCCGTCAAT GCGGGCGGCA ACACCAACGC TGCCGGGGGA
    CCCAATACTG TGCTCCGTGT GATTGTCGAG TCCTTGATGT ACCCCGTCTC GCTGGACATA
    CTGCACCAGA TCTTCCAGCG CTACGGCAAG GTGCTGAAGA TTGTCACCTT CACAAAGAAC
    AATTCATTCC AGGCCCTCAT CCAATATCCG GACGCCAATT CCGCCCAGCA CGCCAAGTCG
    CTCCTGGACG GGCAGAATAT ATACAACGGT TGCTGCACGT TGCGTATTGA TAACAGCAAG
    CTGACGGCTC TGAATGTTAA GTACAACAAC GACAAATCGC GCGACTTCAC TAACCCCGCT
    CTGCCACCTG GTGAACCGGG AGTGGATATC ATGCCCACCG CCGGCGGACT GATGAACACC
    AATGATTTGC TTTTGATCGC CGCCCGTCAG CGACCTTCTC TCTCAGTCAA CGGCCTGGGC
    GCCCCCGGAG TCCTGCCGCC CTTTGCCTTG GGCCTGGGCA CTCCACTGAC CGGTGGATAC
    AACAACGCAC TGCCCAACTT AGCAGCTTTC TCGCTGGCCA ACAGTGGCGC CCTGCAGACG
    ACCGCTCCTG CAATGCGAGG TTACTCGAAT GTCTTGCTTG TCTCGAATTT AAATGAGGAG
    ATGGTCACGC CTGATGCTCT CTTTACCCTT TTTGGTGTAT ACGGCGATGT GCAACGTGTA
    AAGATTCTGT ACAACAAGAA GGACTCGGCA CTCATACAAA TGGCGGAGCC CCAGCAAGCT
    TATTTGGCCA TGTCTCACCT TGATAAATTA CGTTTATGGG GAAAGCCGAT ACGTGTGATG
    GCAAGCAAAC ACCAGGCCGT CCAGCTGCCC AAGGAGGGAC AGCCGGACGC AGGACTCACA
    CGTGACTACT CGCAAAATCC GTTGCACCGC TTCAAGAAAC CGGGAAGCAA GAACTACCAG
    AACATCTATC CGCCGTCGGC GACACTACAT TTAAGCAACA TTCCCTCATC CTGCTCTGAG
    GATGACATCA AAGAAGCTTT CACCTCAAAC AGCTTTGAAG TTAAGGCATT CAAATTTTTC
    CCGAAGGACC GTAAAATGGC ACTGCTTCAA CTGTTGTCCG TGGAGGAGGC TGTCCTAGCA
    CTGATTAAGA TGCACAACCA CCAGCTGTCC GAGTCGAATC ACCTGCGTGT AAGCTTCTCG
    AAGTCGAACA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06932
    Clone ID Related Accession (Same CDS sequence) NM_001170316.2 , NM_001170319.1 , NM_176603.3
    Accession Version NM_001170316.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170316.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATCGC GGAAGCGACG AACTTTTGAG TCAAGCAGCG
    GTCATGGCGC CCGCTTCCGA CAATAACAAT CAGGACCTGG CCACAAAGAA GGCCAAACTG
    GAGCCGGGCA CTGTGCTGGC CGGCGGAATT GCCAAGGCCT CAAAAGTCAT CCACTTGCGC
    AACATTCCGA ACGAGTCCGG CGAGGCAGAT GTGATTGCCC TGGGCATTCC GTTTGGACGT
    GTGACCAACG TGCTGGTGCT CAAGGGCAAG AACCAGGCTT TCATCGAGAT GGCCGACGAG
    ATCTCCGCAA CGTCAATGGT GTCCTGTTAC ACAGTAACTC CGCCCCAGAT GCGCGGCCGC
    ATGGTCTACG TGCAGTTTTC TAATCATCGC GAACTAAAGA CGGACCAAGG TCACAACAAC
    TCGACCGCCC ATAGCGACTA CAGCGTCCAG TCCCCGGCGA GCGGATCGCC TCTGCCCCTT
    AGCGCCGCTG CCAACGCGAC CAGCAACAAT GCAAACAGCT CAAGCGACAG CAACAGCGCC
    ATGGGAATCT TACAAAACAC AAGCGCCGTC AATGCGGGCG GCAACACCAA CGCTGCCGGG
    GGACCCAATA CTGTGCTCCG TGTGATTGTC GAGTCCTTGA TGTACCCCGT CTCGCTGGAC
    ATACTGCACC AGATCTTCCA GCGCTACGGC AAGGTGCTGA AGATTGTCAC CTTCACAAAG
    AACAATTCAT TCCAGGCCCT CATCCAATAT CCGGACGCCA ATTCCGCCCA GCACGCCAAG
    TCGCTCCTGG ACGGGCAGAA TATATACAAC GGTTGCTGCA CGTTGCGTAT TGATAACAGC
    AAGCTGACGG CTCTGAATGT TAAGTACAAC AACGACAAAT CGCGCGACTT CACTAACCCC
    GCTCTGCCAC CTGGTGAACC GGGAGTGGAT ATCATGCCCA CCGCCGGCGG ACTGATGAAC
    ACCAATGATT TGCTTTTGAT CGCCGCCCGT CAGCGACCTT CTCTCTCAGG TGATAAAATA
    GTCAACGGCC TGGGCGCCCC CGGAGTCCTG CCGCCCTTTG CCTTGGGCCT GGGCACTCCA
    CTGACCGGTG GATACAACAA CGCACTGCCC AACTTAGCAG CTTTCTCGCT GGCCAACAGT
    GGCGCCCTGC AGACGACCGC TCCTGCAATG CGAGGTTACT CGAATGTCTT GCTTGTCTCG
    AATTTAAATG AGGAGATGGT CACGCCTGAT GCTCTCTTTA CCCTTTTTGG TGTATACGGC
    GATGTGCAAC GTGTAAAGAT TCTGTACAAC AAGAAGGACT CGGCACTCAT ACAAATGGCG
    GAGCCCCAGC AAGCTTATTT GGCCATGTCT CACCTTGATA AATTACGTTT ATGGGGAAAG
    CCGATACGTG TGATGGCAAG CAAACACCAG GCCGTCCAGC TGCCCAAGGA GGGACAGCCG
    GACGCAGGAC TCACACGTGA CTACTCGCAA AATCCGTTGC ACCGCTTCAA GAAACCGGGA
    AGCAAGAACT ACCAGAACAT CTATCCGCCG TCGGCGACAC TACATTTAAG CAACATTCCC
    TCATCCTGCT CTGAGGATGA CATCAAAGAA GCTTTCACCT CAAACAGCTT TGAAGTTAAG
    GCATTCAAAT TTTTCCCGAA GGACCGTAAA ATGGCACTGC TTCAACTGTT GTCCGTGGAG
    GAGGCTGTCC TAGCACTGAT TAAGATGCAC AACCACCAGC TGTCCGAGTC GAATCACCTG
    CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163787.1
    CDS254..2623
    Translation

    Target ORF information:

    RefSeq Version NM_001170316.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170316.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATCGC GGAAGCGACG AACTTTTGAG TCAAGCAGCG
    GTCATGGCGC CCGCTTCCGA CAATAACAAT CAGGACCTGG CCACAAAGAA GGCCAAACTG
    GAGCCGGGCA CTGTGCTGGC CGGCGGAATT GCCAAGGCCT CAAAAGTCAT CCACTTGCGC
    AACATTCCGA ACGAGTCCGG CGAGGCAGAT GTGATTGCCC TGGGCATTCC GTTTGGACGT
    GTGACCAACG TGCTGGTGCT CAAGGGCAAG AACCAGGCTT TCATCGAGAT GGCCGACGAG
    ATCTCCGCAA CGTCAATGGT GTCCTGTTAC ACAGTAACTC CGCCCCAGAT GCGCGGCCGC
    ATGGTCTACG TGCAGTTTTC TAATCATCGC GAACTAAAGA CGGACCAAGG TCACAACAAC
    TCGACCGCCC ATAGCGACTA CAGCGTCCAG TCCCCGGCGA GCGGATCGCC TCTGCCCCTT
    AGCGCCGCTG CCAACGCGAC CAGCAACAAT GCAAACAGCT CAAGCGACAG CAACAGCGCC
    ATGGGAATCT TACAAAACAC AAGCGCCGTC AATGCGGGCG GCAACACCAA CGCTGCCGGG
    GGACCCAATA CTGTGCTCCG TGTGATTGTC GAGTCCTTGA TGTACCCCGT CTCGCTGGAC
    ATACTGCACC AGATCTTCCA GCGCTACGGC AAGGTGCTGA AGATTGTCAC CTTCACAAAG
    AACAATTCAT TCCAGGCCCT CATCCAATAT CCGGACGCCA ATTCCGCCCA GCACGCCAAG
    TCGCTCCTGG ACGGGCAGAA TATATACAAC GGTTGCTGCA CGTTGCGTAT TGATAACAGC
    AAGCTGACGG CTCTGAATGT TAAGTACAAC AACGACAAAT CGCGCGACTT CACTAACCCC
    GCTCTGCCAC CTGGTGAACC GGGAGTGGAT ATCATGCCCA CCGCCGGCGG ACTGATGAAC
    ACCAATGATT TGCTTTTGAT CGCCGCCCGT CAGCGACCTT CTCTCTCAGG TGATAAAATA
    GTCAACGGCC TGGGCGCCCC CGGAGTCCTG CCGCCCTTTG CCTTGGGCCT GGGCACTCCA
    CTGACCGGTG GATACAACAA CGCACTGCCC AACTTAGCAG CTTTCTCGCT GGCCAACAGT
    GGCGCCCTGC AGACGACCGC TCCTGCAATG CGAGGTTACT CGAATGTCTT GCTTGTCTCG
    AATTTAAATG AGGAGATGGT CACGCCTGAT GCTCTCTTTA CCCTTTTTGG TGTATACGGC
    GATGTGCAAC GTGTAAAGAT TCTGTACAAC AAGAAGGACT CGGCACTCAT ACAAATGGCG
    GAGCCCCAGC AAGCTTATTT GGCCATGTCT CACCTTGATA AATTACGTTT ATGGGGAAAG
    CCGATACGTG TGATGGCAAG CAAACACCAG GCCGTCCAGC TGCCCAAGGA GGGACAGCCG
    GACGCAGGAC TCACACGTGA CTACTCGCAA AATCCGTTGC ACCGCTTCAA GAAACCGGGA
    AGCAAGAACT ACCAGAACAT CTATCCGCCG TCGGCGACAC TACATTTAAG CAACATTCCC
    TCATCCTGCT CTGAGGATGA CATCAAAGAA GCTTTCACCT CAAACAGCTT TGAAGTTAAG
    GCATTCAAAT TTTTCCCGAA GGACCGTAAA ATGGCACTGC TTCAACTGTT GTCCGTGGAG
    GAGGCTGTCC TAGCACTGAT TAAGATGCAC AACCACCAGC TGTCCGAGTC GAATCACCTG
    CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06932
    Clone ID Related Accession (Same CDS sequence) NM_001170316.2 , NM_001170319.1 , NM_176603.3
    Accession Version NM_001170319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform R
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATCGC GGAAGCGACG AACTTTTGAG TCAAGCAGCG
    GTCATGGCGC CCGCTTCCGA CAATAACAAT CAGGACCTGG CCACAAAGAA GGCCAAACTG
    GAGCCGGGCA CTGTGCTGGC CGGCGGAATT GCCAAGGCCT CAAAAGTCAT CCACTTGCGC
    AACATTCCGA ACGAGTCCGG CGAGGCAGAT GTGATTGCCC TGGGCATTCC GTTTGGACGT
    GTGACCAACG TGCTGGTGCT CAAGGGCAAG AACCAGGCTT TCATCGAGAT GGCCGACGAG
    ATCTCCGCAA CGTCAATGGT GTCCTGTTAC ACAGTAACTC CGCCCCAGAT GCGCGGCCGC
    ATGGTCTACG TGCAGTTTTC TAATCATCGC GAACTAAAGA CGGACCAAGG TCACAACAAC
    TCGACCGCCC ATAGCGACTA CAGCGTCCAG TCCCCGGCGA GCGGATCGCC TCTGCCCCTT
    AGCGCCGCTG CCAACGCGAC CAGCAACAAT GCAAACAGCT CAAGCGACAG CAACAGCGCC
    ATGGGAATCT TACAAAACAC AAGCGCCGTC AATGCGGGCG GCAACACCAA CGCTGCCGGG
    GGACCCAATA CTGTGCTCCG TGTGATTGTC GAGTCCTTGA TGTACCCCGT CTCGCTGGAC
    ATACTGCACC AGATCTTCCA GCGCTACGGC AAGGTGCTGA AGATTGTCAC CTTCACAAAG
    AACAATTCAT TCCAGGCCCT CATCCAATAT CCGGACGCCA ATTCCGCCCA GCACGCCAAG
    TCGCTCCTGG ACGGGCAGAA TATATACAAC GGTTGCTGCA CGTTGCGTAT TGATAACAGC
    AAGCTGACGG CTCTGAATGT TAAGTACAAC AACGACAAAT CGCGCGACTT CACTAACCCC
    GCTCTGCCAC CTGGTGAACC GGGAGTGGAT ATCATGCCCA CCGCCGGCGG ACTGATGAAC
    ACCAATGATT TGCTTTTGAT CGCCGCCCGT CAGCGACCTT CTCTCTCAGG TGATAAAATA
    GTCAACGGCC TGGGCGCCCC CGGAGTCCTG CCGCCCTTTG CCTTGGGCCT GGGCACTCCA
    CTGACCGGTG GATACAACAA CGCACTGCCC AACTTAGCAG CTTTCTCGCT GGCCAACAGT
    GGCGCCCTGC AGACGACCGC TCCTGCAATG CGAGGTTACT CGAATGTCTT GCTTGTCTCG
    AATTTAAATG AGGAGATGGT CACGCCTGAT GCTCTCTTTA CCCTTTTTGG TGTATACGGC
    GATGTGCAAC GTGTAAAGAT TCTGTACAAC AAGAAGGACT CGGCACTCAT ACAAATGGCG
    GAGCCCCAGC AAGCTTATTT GGCCATGTCT CACCTTGATA AATTACGTTT ATGGGGAAAG
    CCGATACGTG TGATGGCAAG CAAACACCAG GCCGTCCAGC TGCCCAAGGA GGGACAGCCG
    GACGCAGGAC TCACACGTGA CTACTCGCAA AATCCGTTGC ACCGCTTCAA GAAACCGGGA
    AGCAAGAACT ACCAGAACAT CTATCCGCCG TCGGCGACAC TACATTTAAG CAACATTCCC
    TCATCCTGCT CTGAGGATGA CATCAAAGAA GCTTTCACCT CAAACAGCTT TGAAGTTAAG
    GCATTCAAAT TTTTCCCGAA GGACCGTAAA ATGGCACTGC TTCAACTGTT GTCCGTGGAG
    GAGGCTGTCC TAGCACTGAT TAAGATGCAC AACCACCAGC TGTCCGAGTC GAATCACCTG
    CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163790.1
    CDS292..2661
    Translation

    Target ORF information:

    RefSeq Version NM_001170319.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant R, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATCGC GGAAGCGACG AACTTTTGAG TCAAGCAGCG
    GTCATGGCGC CCGCTTCCGA CAATAACAAT CAGGACCTGG CCACAAAGAA GGCCAAACTG
    GAGCCGGGCA CTGTGCTGGC CGGCGGAATT GCCAAGGCCT CAAAAGTCAT CCACTTGCGC
    AACATTCCGA ACGAGTCCGG CGAGGCAGAT GTGATTGCCC TGGGCATTCC GTTTGGACGT
    GTGACCAACG TGCTGGTGCT CAAGGGCAAG AACCAGGCTT TCATCGAGAT GGCCGACGAG
    ATCTCCGCAA CGTCAATGGT GTCCTGTTAC ACAGTAACTC CGCCCCAGAT GCGCGGCCGC
    ATGGTCTACG TGCAGTTTTC TAATCATCGC GAACTAAAGA CGGACCAAGG TCACAACAAC
    TCGACCGCCC ATAGCGACTA CAGCGTCCAG TCCCCGGCGA GCGGATCGCC TCTGCCCCTT
    AGCGCCGCTG CCAACGCGAC CAGCAACAAT GCAAACAGCT CAAGCGACAG CAACAGCGCC
    ATGGGAATCT TACAAAACAC AAGCGCCGTC AATGCGGGCG GCAACACCAA CGCTGCCGGG
    GGACCCAATA CTGTGCTCCG TGTGATTGTC GAGTCCTTGA TGTACCCCGT CTCGCTGGAC
    ATACTGCACC AGATCTTCCA GCGCTACGGC AAGGTGCTGA AGATTGTCAC CTTCACAAAG
    AACAATTCAT TCCAGGCCCT CATCCAATAT CCGGACGCCA ATTCCGCCCA GCACGCCAAG
    TCGCTCCTGG ACGGGCAGAA TATATACAAC GGTTGCTGCA CGTTGCGTAT TGATAACAGC
    AAGCTGACGG CTCTGAATGT TAAGTACAAC AACGACAAAT CGCGCGACTT CACTAACCCC
    GCTCTGCCAC CTGGTGAACC GGGAGTGGAT ATCATGCCCA CCGCCGGCGG ACTGATGAAC
    ACCAATGATT TGCTTTTGAT CGCCGCCCGT CAGCGACCTT CTCTCTCAGG TGATAAAATA
    GTCAACGGCC TGGGCGCCCC CGGAGTCCTG CCGCCCTTTG CCTTGGGCCT GGGCACTCCA
    CTGACCGGTG GATACAACAA CGCACTGCCC AACTTAGCAG CTTTCTCGCT GGCCAACAGT
    GGCGCCCTGC AGACGACCGC TCCTGCAATG CGAGGTTACT CGAATGTCTT GCTTGTCTCG
    AATTTAAATG AGGAGATGGT CACGCCTGAT GCTCTCTTTA CCCTTTTTGG TGTATACGGC
    GATGTGCAAC GTGTAAAGAT TCTGTACAAC AAGAAGGACT CGGCACTCAT ACAAATGGCG
    GAGCCCCAGC AAGCTTATTT GGCCATGTCT CACCTTGATA AATTACGTTT ATGGGGAAAG
    CCGATACGTG TGATGGCAAG CAAACACCAG GCCGTCCAGC TGCCCAAGGA GGGACAGCCG
    GACGCAGGAC TCACACGTGA CTACTCGCAA AATCCGTTGC ACCGCTTCAA GAAACCGGGA
    AGCAAGAACT ACCAGAACAT CTATCCGCCG TCGGCGACAC TACATTTAAG CAACATTCCC
    TCATCCTGCT CTGAGGATGA CATCAAAGAA GCTTTCACCT CAAACAGCTT TGAAGTTAAG
    GCATTCAAAT TTTTCCCGAA GGACCGTAAA ATGGCACTGC TTCAACTGTT GTCCGTGGAG
    GAGGCTGTCC TAGCACTGAT TAAGATGCAC AACCACCAGC TGTCCGAGTC GAATCACCTG
    CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06932
    Clone ID Related Accession (Same CDS sequence) NM_001170316.2 , NM_001170319.1 , NM_176603.3
    Accession Version NM_176603.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_176603.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATCGC GGAAGCGACG AACTTTTGAG TCAAGCAGCG
    GTCATGGCGC CCGCTTCCGA CAATAACAAT CAGGACCTGG CCACAAAGAA GGCCAAACTG
    GAGCCGGGCA CTGTGCTGGC CGGCGGAATT GCCAAGGCCT CAAAAGTCAT CCACTTGCGC
    AACATTCCGA ACGAGTCCGG CGAGGCAGAT GTGATTGCCC TGGGCATTCC GTTTGGACGT
    GTGACCAACG TGCTGGTGCT CAAGGGCAAG AACCAGGCTT TCATCGAGAT GGCCGACGAG
    ATCTCCGCAA CGTCAATGGT GTCCTGTTAC ACAGTAACTC CGCCCCAGAT GCGCGGCCGC
    ATGGTCTACG TGCAGTTTTC TAATCATCGC GAACTAAAGA CGGACCAAGG TCACAACAAC
    TCGACCGCCC ATAGCGACTA CAGCGTCCAG TCCCCGGCGA GCGGATCGCC TCTGCCCCTT
    AGCGCCGCTG CCAACGCGAC CAGCAACAAT GCAAACAGCT CAAGCGACAG CAACAGCGCC
    ATGGGAATCT TACAAAACAC AAGCGCCGTC AATGCGGGCG GCAACACCAA CGCTGCCGGG
    GGACCCAATA CTGTGCTCCG TGTGATTGTC GAGTCCTTGA TGTACCCCGT CTCGCTGGAC
    ATACTGCACC AGATCTTCCA GCGCTACGGC AAGGTGCTGA AGATTGTCAC CTTCACAAAG
    AACAATTCAT TCCAGGCCCT CATCCAATAT CCGGACGCCA ATTCCGCCCA GCACGCCAAG
    TCGCTCCTGG ACGGGCAGAA TATATACAAC GGTTGCTGCA CGTTGCGTAT TGATAACAGC
    AAGCTGACGG CTCTGAATGT TAAGTACAAC AACGACAAAT CGCGCGACTT CACTAACCCC
    GCTCTGCCAC CTGGTGAACC GGGAGTGGAT ATCATGCCCA CCGCCGGCGG ACTGATGAAC
    ACCAATGATT TGCTTTTGAT CGCCGCCCGT CAGCGACCTT CTCTCTCAGG TGATAAAATA
    GTCAACGGCC TGGGCGCCCC CGGAGTCCTG CCGCCCTTTG CCTTGGGCCT GGGCACTCCA
    CTGACCGGTG GATACAACAA CGCACTGCCC AACTTAGCAG CTTTCTCGCT GGCCAACAGT
    GGCGCCCTGC AGACGACCGC TCCTGCAATG CGAGGTTACT CGAATGTCTT GCTTGTCTCG
    AATTTAAATG AGGAGATGGT CACGCCTGAT GCTCTCTTTA CCCTTTTTGG TGTATACGGC
    GATGTGCAAC GTGTAAAGAT TCTGTACAAC AAGAAGGACT CGGCACTCAT ACAAATGGCG
    GAGCCCCAGC AAGCTTATTT GGCCATGTCT CACCTTGATA AATTACGTTT ATGGGGAAAG
    CCGATACGTG TGATGGCAAG CAAACACCAG GCCGTCCAGC TGCCCAAGGA GGGACAGCCG
    GACGCAGGAC TCACACGTGA CTACTCGCAA AATCCGTTGC ACCGCTTCAA GAAACCGGGA
    AGCAAGAACT ACCAGAACAT CTATCCGCCG TCGGCGACAC TACATTTAAG CAACATTCCC
    TCATCCTGCT CTGAGGATGA CATCAAAGAA GCTTTCACCT CAAACAGCTT TGAAGTTAAG
    GCATTCAAAT TTTTCCCGAA GGACCGTAAA ATGGCACTGC TTCAACTGTT GTCCGTGGAG
    GAGGCTGTCC TAGCACTGAT TAAGATGCAC AACCACCAGC TGTCCGAGTC GAATCACCTG
    CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788780.1
    CDS254..2623
    Translation

    Target ORF information:

    RefSeq Version NM_176603.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176603.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATCGC GGAAGCGACG AACTTTTGAG TCAAGCAGCG
    GTCATGGCGC CCGCTTCCGA CAATAACAAT CAGGACCTGG CCACAAAGAA GGCCAAACTG
    GAGCCGGGCA CTGTGCTGGC CGGCGGAATT GCCAAGGCCT CAAAAGTCAT CCACTTGCGC
    AACATTCCGA ACGAGTCCGG CGAGGCAGAT GTGATTGCCC TGGGCATTCC GTTTGGACGT
    GTGACCAACG TGCTGGTGCT CAAGGGCAAG AACCAGGCTT TCATCGAGAT GGCCGACGAG
    ATCTCCGCAA CGTCAATGGT GTCCTGTTAC ACAGTAACTC CGCCCCAGAT GCGCGGCCGC
    ATGGTCTACG TGCAGTTTTC TAATCATCGC GAACTAAAGA CGGACCAAGG TCACAACAAC
    TCGACCGCCC ATAGCGACTA CAGCGTCCAG TCCCCGGCGA GCGGATCGCC TCTGCCCCTT
    AGCGCCGCTG CCAACGCGAC CAGCAACAAT GCAAACAGCT CAAGCGACAG CAACAGCGCC
    ATGGGAATCT TACAAAACAC AAGCGCCGTC AATGCGGGCG GCAACACCAA CGCTGCCGGG
    GGACCCAATA CTGTGCTCCG TGTGATTGTC GAGTCCTTGA TGTACCCCGT CTCGCTGGAC
    ATACTGCACC AGATCTTCCA GCGCTACGGC AAGGTGCTGA AGATTGTCAC CTTCACAAAG
    AACAATTCAT TCCAGGCCCT CATCCAATAT CCGGACGCCA ATTCCGCCCA GCACGCCAAG
    TCGCTCCTGG ACGGGCAGAA TATATACAAC GGTTGCTGCA CGTTGCGTAT TGATAACAGC
    AAGCTGACGG CTCTGAATGT TAAGTACAAC AACGACAAAT CGCGCGACTT CACTAACCCC
    GCTCTGCCAC CTGGTGAACC GGGAGTGGAT ATCATGCCCA CCGCCGGCGG ACTGATGAAC
    ACCAATGATT TGCTTTTGAT CGCCGCCCGT CAGCGACCTT CTCTCTCAGG TGATAAAATA
    GTCAACGGCC TGGGCGCCCC CGGAGTCCTG CCGCCCTTTG CCTTGGGCCT GGGCACTCCA
    CTGACCGGTG GATACAACAA CGCACTGCCC AACTTAGCAG CTTTCTCGCT GGCCAACAGT
    GGCGCCCTGC AGACGACCGC TCCTGCAATG CGAGGTTACT CGAATGTCTT GCTTGTCTCG
    AATTTAAATG AGGAGATGGT CACGCCTGAT GCTCTCTTTA CCCTTTTTGG TGTATACGGC
    GATGTGCAAC GTGTAAAGAT TCTGTACAAC AAGAAGGACT CGGCACTCAT ACAAATGGCG
    GAGCCCCAGC AAGCTTATTT GGCCATGTCT CACCTTGATA AATTACGTTT ATGGGGAAAG
    CCGATACGTG TGATGGCAAG CAAACACCAG GCCGTCCAGC TGCCCAAGGA GGGACAGCCG
    GACGCAGGAC TCACACGTGA CTACTCGCAA AATCCGTTGC ACCGCTTCAA GAAACCGGGA
    AGCAAGAACT ACCAGAACAT CTATCCGCCG TCGGCGACAC TACATTTAAG CAACATTCCC
    TCATCCTGCT CTGAGGATGA CATCAAAGAA GCTTTCACCT CAAACAGCTT TGAAGTTAAG
    GCATTCAAAT TTTTCCCGAA GGACCGTAAA ATGGCACTGC TTCAACTGTT GTCCGTGGAG
    GAGGCTGTCC TAGCACTGAT TAAGATGCAC AACCACCAGC TGTCCGAGTC GAATCACCTG
    CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06933
    Clone ID Related Accession (Same CDS sequence) NM_001260469.2
    Accession Version NM_001260469.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform W
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260469.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATGTA AGGATCGACG AACAATATAT ATATAAACTG
    AACTATTTGC GCGGAAGCGA CGAACTTTTG AGTCAAGCAG CGGTCATGGC GCCCGCTTCC
    GACAATAACA ATCAGGACCT GGCCACAAAG AAGGCCAAAC TGGAGCCGGG CACTGTGCTG
    GCCGGCGGAA TTGCCAAGGC CTCAAAAGTC ATCCACTTGC GCAACATTCC GAACGAGTCC
    GGCGAGGCAG ATGTGATTGC CCTGGGCATT CCGTTTGGAC GTGTGACCAA CGTGCTGGTG
    CTCAAGGGCA AGAACCAGGC TTTCATCGAG ATGGCCGACG AGATCTCCGC AACGTCAATG
    GTGTCCTGTT ACACAGTAAC TCCGCCCCAG ATGCGCGGCC GCATGGTCTA CGTGCAGTTT
    TCTAATCATC GCGAACTAAA GACGGACCAA GGTCACAACA ACTCGACCGC CCATAGCGAC
    TACAGCGTCC AGTCCCCGGC GAGCGGATCG CCTCTGCCCC TTAGCGCCGC TGCCAACGCG
    ACCAGCAACA ATGCAAACAG CTCAAGCGAC AGCAACAGCG CCATGGGAAT CTTACAAAAC
    ACAAGCGCCG TCAATGCGGG CGGCAACACC AACGCTGCCG GGGGACCCAA TACTGTGCTC
    CGTGTGATTG TCGAGTCCTT GATGTACCCC GTCTCGCTGG ACATACTGCA CCAGATCTTC
    CAGCGCTACG GCAAGGTGCT GAAGATTGTC ACCTTCACAA AGAACAATTC ATTCCAGGCC
    CTCATCCAAT ATCCGGACGC CAATTCCGCC CAGCACGCCA AGTCGCTCCT GGACGGGCAG
    AATATATACA ACGGTTGCTG CACGTTGCGT ATTGATAACA GCAAGCTGAC GGCTCTGAAT
    GTTAAGTACA ACAACGACAA ATCGCGCGAC TTCACTAACC CCGCTCTGCC ACCTGGTGAA
    CCGGGAGTGG ATATCATGCC CACCGCCGGC GGACTGATGA ACACCAATGA TTTGCTTTTG
    ATCGCCGCCC GTCAGCGACC TTCTCTCTCA GTCAACGGCC TGGGCGCCCC CGGAGTCCTG
    CCGCCCTTTG CCTTGGGCCT GGGCACTCCA CTGACCGGTG GATACAACAA CGCACTGCCC
    AACTTAGCAG CTTTCTCGCT GGCCAACAGT GGCGCCCTGC AGACGACCGC TCCTGCAATG
    CGAGGTTACT CGAATGTCTT GCTTGTCTCG AATTTAAATG AGGAGATGGT CACGCCTGAT
    GCTCTCTTTA CCCTTTTTGG TGTATACGGC GATGTGCAAC GTGTAAAGAT TCTGTACAAC
    AAGAAGGACT CGGCACTCAT ACAAATGGCG GAGCCCCAGC AAGCTTATTT GGCCATGTCT
    CACCTTGATA AATTACGTTT ATGGGGAAAG CCGATACGTG TGATGGCAAG CAAACACCAG
    GCCGTCCAGC TGCCCAAGGA GGGACAGCCG GACGCAGGAC TCACACGTGA CTACTCGCAA
    AATCCGTTGC ACCGCTTCAA GAAACCGGGA AGCAAGAACT ACCAGAACAT CTATCCGCCG
    TCGGCGACAC TACATTTAAG CAACATTCCC TCATCCTGCT CTGAGGATGA CATCAAAGAA
    GCTTTCACCT CAAACAGCTT TGAAGTTAAG GCATTCAAAT TTTTCCCGAA GGACCGTAAA
    ATGGCACTGC TTCAACTGTT GTCCGTGGAG GAGGCTGTCC TAGCACTGAT TAAGATGCAC
    AACCACCAGC TGTCCGAGTC GAATCACCTG CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247398.1
    CDS417..2816
    Translation

    Target ORF information:

    RefSeq Version NM_001260469.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant W, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260469.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATGTA AGGATCGACG AACAATATAT ATATAAACTG
    AACTATTTGC GCGGAAGCGA CGAACTTTTG AGTCAAGCAG CGGTCATGGC GCCCGCTTCC
    GACAATAACA ATCAGGACCT GGCCACAAAG AAGGCCAAAC TGGAGCCGGG CACTGTGCTG
    GCCGGCGGAA TTGCCAAGGC CTCAAAAGTC ATCCACTTGC GCAACATTCC GAACGAGTCC
    GGCGAGGCAG ATGTGATTGC CCTGGGCATT CCGTTTGGAC GTGTGACCAA CGTGCTGGTG
    CTCAAGGGCA AGAACCAGGC TTTCATCGAG ATGGCCGACG AGATCTCCGC AACGTCAATG
    GTGTCCTGTT ACACAGTAAC TCCGCCCCAG ATGCGCGGCC GCATGGTCTA CGTGCAGTTT
    TCTAATCATC GCGAACTAAA GACGGACCAA GGTCACAACA ACTCGACCGC CCATAGCGAC
    TACAGCGTCC AGTCCCCGGC GAGCGGATCG CCTCTGCCCC TTAGCGCCGC TGCCAACGCG
    ACCAGCAACA ATGCAAACAG CTCAAGCGAC AGCAACAGCG CCATGGGAAT CTTACAAAAC
    ACAAGCGCCG TCAATGCGGG CGGCAACACC AACGCTGCCG GGGGACCCAA TACTGTGCTC
    CGTGTGATTG TCGAGTCCTT GATGTACCCC GTCTCGCTGG ACATACTGCA CCAGATCTTC
    CAGCGCTACG GCAAGGTGCT GAAGATTGTC ACCTTCACAA AGAACAATTC ATTCCAGGCC
    CTCATCCAAT ATCCGGACGC CAATTCCGCC CAGCACGCCA AGTCGCTCCT GGACGGGCAG
    AATATATACA ACGGTTGCTG CACGTTGCGT ATTGATAACA GCAAGCTGAC GGCTCTGAAT
    GTTAAGTACA ACAACGACAA ATCGCGCGAC TTCACTAACC CCGCTCTGCC ACCTGGTGAA
    CCGGGAGTGG ATATCATGCC CACCGCCGGC GGACTGATGA ACACCAATGA TTTGCTTTTG
    ATCGCCGCCC GTCAGCGACC TTCTCTCTCA GTCAACGGCC TGGGCGCCCC CGGAGTCCTG
    CCGCCCTTTG CCTTGGGCCT GGGCACTCCA CTGACCGGTG GATACAACAA CGCACTGCCC
    AACTTAGCAG CTTTCTCGCT GGCCAACAGT GGCGCCCTGC AGACGACCGC TCCTGCAATG
    CGAGGTTACT CGAATGTCTT GCTTGTCTCG AATTTAAATG AGGAGATGGT CACGCCTGAT
    GCTCTCTTTA CCCTTTTTGG TGTATACGGC GATGTGCAAC GTGTAAAGAT TCTGTACAAC
    AAGAAGGACT CGGCACTCAT ACAAATGGCG GAGCCCCAGC AAGCTTATTT GGCCATGTCT
    CACCTTGATA AATTACGTTT ATGGGGAAAG CCGATACGTG TGATGGCAAG CAAACACCAG
    GCCGTCCAGC TGCCCAAGGA GGGACAGCCG GACGCAGGAC TCACACGTGA CTACTCGCAA
    AATCCGTTGC ACCGCTTCAA GAAACCGGGA AGCAAGAACT ACCAGAACAT CTATCCGCCG
    TCGGCGACAC TACATTTAAG CAACATTCCC TCATCCTGCT CTGAGGATGA CATCAAAGAA
    GCTTTCACCT CAAACAGCTT TGAAGTTAAG GCATTCAAAT TTTTCCCGAA GGACCGTAAA
    ATGGCACTGC TTCAACTGTT GTCCGTGGAG GAGGCTGTCC TAGCACTGAT TAAGATGCAC
    AACCACCAGC TGTCCGAGTC GAATCACCTG CGTGTAAGCT TCTCGAAGTC GAACATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06934
    Clone ID Related Accession (Same CDS sequence) NM_001260470.1
    Accession Version NM_001260470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2697bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product hephaestus, isoform Y
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATGTA AATAATACAG TTAATCTATC AGCCGTATCT
    AGAACTACTA CCACTACTAC TAAGAAGCAG CCGCAGGCAG TGGCGGATGC AGTCCTGAGC
    GCCGTCGGCG GTAATGTTGG CACCGCAGTG GGTGGTGGTA CAATATCCTC GCTCCTGGGG
    CCAGAACCAC TGCCGATTAT GCCCCACCAC TTACCACTGG CCAGCGTTCG GGGCTGCAGC
    AGCGCCAGCA GCGGCTGCAG TTCTGCATCT TCTGATTGTT CTATGCCATC GCCAACTTCA
    CTACACTTAT CTTCTCCTAA AAAGGTAAGG ATCGACGAAC AATATATATA TAAACTGAAC
    TATTTGCGCG GAAGCGACGA ACTTTTGAGT CAAGCAGCGG TCATGGCGCC CGCTTCCGAC
    AATAACAATC AGGACCTGGC CACAAAGAAG GCCAAACTGG AGCCGGGCAC TGTGCTGGCC
    GGCGGAATTG CCAAGGCCTC AAAAGTCATC CACTTGCGCA ACATTCCGAA CGAGTCCGGC
    GAGGCAGATG TGATTGCCCT GGGCATTCCG TTTGGACGTG TGACCAACGT GCTGGTGCTC
    AAGGGCAAGA ACCAGGCTTT CATCGAGATG GCCGACGAGA TCTCCGCAAC GTCAATGGTG
    TCCTGTTACA CAGTAACTCC GCCCCAGATG CGCGGCCGCA TGGTCTACGT GCAGTTTTCT
    AATCATCGCG AACTAAAGAC GGACCAAGGT CACAACAACT CGACCGCCCA TAGCGACTAC
    AGCGTCCAGT CCCCGGCGAG CGGATCGCCT CTGCCCCTTA GCGCCGCTGC CAACGCGACC
    AGCAACAATG CAAACAGCTC AAGCGACAGC AACAGCGCCA TGGGAATCTT ACAAAACACA
    AGCGCCGTCA ATGCGGGCGG CAACACCAAC GCTGCCGGGG GACCCAATAC TGTGCTCCGT
    GTGATTGTCG AGTCCTTGAT GTACCCCGTC TCGCTGGACA TACTGCACCA GATCTTCCAG
    CGCTACGGCA AGGTGCTGAA GATTGTCACC TTCACAAAGA ACAATTCATT CCAGGCCCTC
    ATCCAATATC CGGACGCCAA TTCCGCCCAG CACGCCAAGT CGCTCCTGGA CGGGCAGAAT
    ATATACAACG GTTGCTGCAC GTTGCGTATT GATAACAGCA AGCTGACGGC TCTGAATGTT
    AAGTACAACA ACGACAAATC GCGCGACTTC ACTAACCCCG CTCTGCCACC TGGTGAACCG
    GGAGTGGATA TCATGCCCAC CGCCGGCGGA CTGATGAACA CCAATGATTT GCTTTTGATC
    GCCGCCCGTC AGCGACCTTC TCTCTCAGTC AACGGCCTGG GCGCCCCCGG AGTCCTGCCG
    CCCTTTGCCT TGGGCCTGGG CACTCCACTG ACCGGTGGAT ACAACAACGC ACTGCCCAAC
    TTAGCAGCTT TCTCGCTGGC CAACAGTGGC GCCCTGCAGA CGACCGCTCC TGCAATGCGA
    GGTTACTCGA ATGTCTTGCT TGTCTCGAAT TTAAATGAGG AGATGGTCAC GCCTGATGCT
    CTCTTTACCC TTTTTGGTGT ATACGGCGAT GTGCAACGTG TAAAGATTCT GTACAACAAG
    AAGGACTCGG CACTCATACA AATGGCGGAG CCCCAGCAAG CTTATTTGGC CATGTCTCAC
    CTTGATAAAT TACGTTTATG GGGAAAGCCG ATACGTGTGA TGGCAAGCAA ACACCAGGCC
    GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC GCAGGACTCA CACGTGACTA CTCGCAAAAT
    CCGTTGCACC GCTTCAAGAA ACCGGGAAGC AAGAACTACC AGAACATCTA TCCGCCGTCG
    GCGACACTAC ATTTAAGCAA CATTCCCTCA TCCTGCTCTG AGGATGACAT CAAAGAAGCT
    TTCACCTCAA ACAGCTTTGA AGTTAAGGCA TTCAAATTTT TCCCGAAGGA CCGTAAAATG
    GCACTGCTTC AACTGTTGTC CGTGGAGGAG GCTGTCCTAG CACTGATTAA GATGCACAAC
    CACCAGCTGT CCGAGTCGAA TCACCTGCGT GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247399.1
    CDS338..3034
    Translation

    Target ORF information:

    RefSeq Version NM_001260470.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster hephaestus (heph), transcript variant Y, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGTCCT GCCCCATTCC CATGCCAATG CCAATTTCTT CATTACACAA ATCAGAATTT 
    GAACCGGTTA ATATTGGAGT TAAGCAAATG CAATTGTTGC ACGCACCGGC GTCCACGCAT
    CCGCATCCAC ATCCGCATCA GCATCCGCAG CATTCGGCAG CGTCAGCCGC CGCCTATCAG
    GCCATGCAGG TGCACCAGCA GCAACACCAA CAGCAACAGC AGGCGGCCGT CTTTCACCAC
    CAGGTTGCAG CCATTGCCGC CCAGCAGCAA CAACAGCAAC AGCAGGCCCA AGTGCAGCAT
    CAGCAACAAA TGCAGCAGCA GTTCCACTAC CAAGCCCAGG CTCAAGCTCA AGCGCAGGCC
    ATGCTGCAGA CGTATATGCA ACTTCAGGTG AACAGTGCTG TAGGTGTGAG CACCGCCCCC
    GCCACGCCTA CCAAACCGGT AGCCGCCCAG ATTCCGGGCG CGATCTCCGG ACCGATTGCG
    GGTCCGGTTC CTGTATCTGT GCCGACACAG TTCTTCACGG CGCCGAGCAC TCCATTGACC
    ATCATTCCGG CGGCGGCGGT GGCCTCGTCG GCCATGTTCG CTACCGCGGC CACGCCCACT
    TCGGCGGCCA CCACTCCGAC CAATAATGTA AATAATACAG TTAATCTATC AGCCGTATCT
    AGAACTACTA CCACTACTAC TAAGAAGCAG CCGCAGGCAG TGGCGGATGC AGTCCTGAGC
    GCCGTCGGCG GTAATGTTGG CACCGCAGTG GGTGGTGGTA CAATATCCTC GCTCCTGGGG
    CCAGAACCAC TGCCGATTAT GCCCCACCAC TTACCACTGG CCAGCGTTCG GGGCTGCAGC
    AGCGCCAGCA GCGGCTGCAG TTCTGCATCT TCTGATTGTT CTATGCCATC GCCAACTTCA
    CTACACTTAT CTTCTCCTAA AAAGGTAAGG ATCGACGAAC AATATATATA TAAACTGAAC
    TATTTGCGCG GAAGCGACGA ACTTTTGAGT CAAGCAGCGG TCATGGCGCC CGCTTCCGAC
    AATAACAATC AGGACCTGGC CACAAAGAAG GCCAAACTGG AGCCGGGCAC TGTGCTGGCC
    GGCGGAATTG CCAAGGCCTC AAAAGTCATC CACTTGCGCA ACATTCCGAA CGAGTCCGGC
    GAGGCAGATG TGATTGCCCT GGGCATTCCG TTTGGACGTG TGACCAACGT GCTGGTGCTC
    AAGGGCAAGA ACCAGGCTTT CATCGAGATG GCCGACGAGA TCTCCGCAAC GTCAATGGTG
    TCCTGTTACA CAGTAACTCC GCCCCAGATG CGCGGCCGCA TGGTCTACGT GCAGTTTTCT
    AATCATCGCG AACTAAAGAC GGACCAAGGT CACAACAACT CGACCGCCCA TAGCGACTAC
    AGCGTCCAGT CCCCGGCGAG CGGATCGCCT CTGCCCCTTA GCGCCGCTGC CAACGCGACC
    AGCAACAATG CAAACAGCTC AAGCGACAGC AACAGCGCCA TGGGAATCTT ACAAAACACA
    AGCGCCGTCA ATGCGGGCGG CAACACCAAC GCTGCCGGGG GACCCAATAC TGTGCTCCGT
    GTGATTGTCG AGTCCTTGAT GTACCCCGTC TCGCTGGACA TACTGCACCA GATCTTCCAG
    CGCTACGGCA AGGTGCTGAA GATTGTCACC TTCACAAAGA ACAATTCATT CCAGGCCCTC
    ATCCAATATC CGGACGCCAA TTCCGCCCAG CACGCCAAGT CGCTCCTGGA CGGGCAGAAT
    ATATACAACG GTTGCTGCAC GTTGCGTATT GATAACAGCA AGCTGACGGC TCTGAATGTT
    AAGTACAACA ACGACAAATC GCGCGACTTC ACTAACCCCG CTCTGCCACC TGGTGAACCG
    GGAGTGGATA TCATGCCCAC CGCCGGCGGA CTGATGAACA CCAATGATTT GCTTTTGATC
    GCCGCCCGTC AGCGACCTTC TCTCTCAGTC AACGGCCTGG GCGCCCCCGG AGTCCTGCCG
    CCCTTTGCCT TGGGCCTGGG CACTCCACTG ACCGGTGGAT ACAACAACGC ACTGCCCAAC
    TTAGCAGCTT TCTCGCTGGC CAACAGTGGC GCCCTGCAGA CGACCGCTCC TGCAATGCGA
    GGTTACTCGA ATGTCTTGCT TGTCTCGAAT TTAAATGAGG AGATGGTCAC GCCTGATGCT
    CTCTTTACCC TTTTTGGTGT ATACGGCGAT GTGCAACGTG TAAAGATTCT GTACAACAAG
    AAGGACTCGG CACTCATACA AATGGCGGAG CCCCAGCAAG CTTATTTGGC CATGTCTCAC
    CTTGATAAAT TACGTTTATG GGGAAAGCCG ATACGTGTGA TGGCAAGCAA ACACCAGGCC
    GTCCAGCTGC CCAAGGAGGG ACAGCCGGAC GCAGGACTCA CACGTGACTA CTCGCAAAAT
    CCGTTGCACC GCTTCAAGAA ACCGGGAAGC AAGAACTACC AGAACATCTA TCCGCCGTCG
    GCGACACTAC ATTTAAGCAA CATTCCCTCA TCCTGCTCTG AGGATGACAT CAAAGAAGCT
    TTCACCTCAA ACAGCTTTGA AGTTAAGGCA TTCAAATTTT TCCCGAAGGA CCGTAAAATG
    GCACTGCTTC AACTGTTGTC CGTGGAGGAG GCTGTCCTAG CACTGATTAA GATGCACAAC
    CACCAGCTGT CCGAGTCGAA TCACCTGCGT GTAAGCTTCT CGAAGTCGAA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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