TRPC3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Gene Symbol	TRPC3
Entrez Gene ID	374153
Full Name	transient receptor potential cation channel subfamily C member 3
General protein information	Preferred Names transient receptor potential cation channel subfamily C member 3 Names short transient receptor potential channel 3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015276103.2	XP_015131589.1	short transient receptor potential channel 3 isoform X1
NM_001257279.3	NP_001244208.1	short transient receptor potential channel 3
XM_015276103.1	XP_015131589.1	short transient receptor potential channel 3 isoform X1
NM_001257279.1	NP_001244208.1	short transient receptor potential channel 3
NM_001257279.2	NP_001244208.1	short transient receptor potential channel 3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	trpc3	XP_002941939.2
Homo sapiens (human)	TRPC3	NP_001124170.1
Pan troglodytes (chimpanzee)	TRPC3	XP_526676.2
Bos taurus (cattle)	TRPC3	NP_001098430.1
Mus musculus (house mouse)	Trpc3	NP_062383.2
	TRPC3	XP_002808466.1
Canis lupus familiaris (dog)	TRPC3	XP_540964.3
Rattus norvegicus (Norway rat)	Trpc3	NP_068539.2
Gallus gallus (chicken)	TRPC3	NP_001244208.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of transient receptor potential canonical 3 (TRPC3)-mediated Ca2+ entry by A1 adenosine receptor in cardiomyocytes disturbs atrioventricular conduction.
Sabourin J, Antigny F, Robin E, Frieden M, Raddatz E
The Journal of biological chemistry287(32)26688-701(2012 Aug)

Ca2+ influx and protein scaffolding via TRPC3 sustain PKCbeta and ERK activation in B cells.
Numaga T, Nishida M, Kiyonaka S, Kato K, Katano M, Mori E, Kurosaki T, Inoue R, Hikida M, Putney JW Jr, Mori Y
Journal of cell science123(Pt 6)927-38(2010 Mar)

TRPC3-like protein and vitamin D receptor mediate 1alpha,25(OH)2D3-induced SOC influx in muscle cells.
Santill?n G, Katz S, Vazquez G, Boland RL
The international journal of biochemistry & cell biology36(10)1910-8(2004 Oct)

Evidence that TRPC3 is a molecular component of the 1alpha,25(OH)2D3-activated capacitative calcium entry (CCE) in muscle and osteoblast cells.
Santill?n G, Baldi C, Katz S, Vazquez G, Boland R
The Journal of steroid biochemistry and molecular biology89-90(1-5)291-5(2004 May)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data showing that A(1)AR activation subtly mediates a proarrhythmic Ca(2+) entry through TRPC3-encoded ROC by stimulating the phospholipase C/DAG/PKC cascade provide evidence for a novel pathway whereby Ca(2+) entry and cardiac function are altered
Title: Activation of transient receptor potential canonical 3 (TRPC3)-mediated Ca2+ entry by A1 adenosine receptor in cardiomyocytes disturbs atrioventricular conduction.

TRPC3 functions as both a Ca(2+)-permeable channel and a protein scaffold at the plasma membrane for downstream PKCbeta activation in B cells.
Title: Ca2+ influx and protein scaffolding via TRPC3 sustain PKCbeta and ERK activation in B cells.

endogenous TRPC3-like proteins and the VDR participate in the modulation of CCE by 1alpha,25(OH)2D3 in muscle cells, which could be mediated by an interaction between these proteins
Title: TRPC3-like protein and vitamin D receptor mediate 1alpha,25(OH)2D3-induced SOC influx in muscle cells.

The following TRPC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa141666	NM_001257279.2 Latest version!	Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa25071	NM_001257279.3 Latest version!	Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa149990	XM_015276103.2 Latest version!	Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa35000	XM_015276103.1 Latest version!	Gallus gallus transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OGa25071	NM_001257279.1	Gallus gallus transient receptor potential cation channel, subfamily C, member 3 (TRPC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPC3 ( NM_001257279.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001244208.1 Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), mRNA.

Price & Availability↑

CloneID	OGa141666
Clone ID Related Accession (Same CDS sequence)	NM_001257279.2
Accession Version	NM_001257279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-29
Organism	Gallus gallus(chicken)
Product	short transient receptor potential channel 3
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AADN04000018.1. On Jun 30, 2017 this sequence version replaced NM_001257279.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201363, SAMEA2201366 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA AGAGCCGGAA GAGCAAGGAA CAGGGGAGAG TGACCTTCCC CCCGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAG GAGGAGGAGG AGGAGGAGGC AGAGGAAGAA 
GAGCGGCAGC GCCGGCGCCG CGGCTGGAGA GGCGTCAACG GTGGGCTGGA GCCCCCGCCG 
CCGCCCCTGC GCGCCGTGAA GACCCTCCGG GAGCCCTACG GCTACTACCC GCCGCTGCCC 
TTCGCCAGCA CCATGTACAT TGAAGAAAGT CCGTCTCTGA GGCGTATGAC TATGATGAGG 
GAAAAGGGAA GGCGCCAGGC CATTAGAGGC CCAGCATTCA TGTTCAACAA CAGAGGCACT 
AGTCTGACTG CTGAGGAAGA GCGCTTCCTA GATGCAGCAG AGTATGGGAA CATACCTGTG 
GTGCGCAAAA TGCTGGAGGA GTCAAAAACA CTGAATGTCA ACTGTGTTGA CTACATGGGC 
CAAAACGCCT TGCAGCTTGC TGTAGGGAAC GAACATTTGG AAGTGACAGA ACTTCTGTTA 
AAAAAAGAGA ACTTGGCACG GATTGGAGAT GCCCTGCTGC TCGCAATCAG CAAGGGCTAC 
ATTAGGATAG TGGAAGCAAT TTTAAATCAT CCTGGGTTTT CAGTAAATAA GCGACTGACT 
CTGAGCCCTT GTGAGCAGGA GCTCCAGGAT GACGATTTTT ATTCCTATGA CGAAGACGGC 
ACCCGCTTTT CTCCAGACAT CACTCCCATC ATTTTAGCTG CTCACTGCCA AAAATACGAA 
GTCGTGCATA TGCTGTTGAT GAAAGGAGCA AGGATAGAAA GGCCTCATGA TTATTTCTGC 
AAATGTAATG ACTGTACAGA GAAGCAAAAG CATGATTCTT TCAGTCACTC TCGGTCAAGA 
ATAAATGCCT ACAAAGGACT GGCCAGTCCA GCTTACTTGT CTCTCTCCAG TGAAGACCCT 
GTGCTCACTG CTCTGGAACT CAGCAATGAG CTTGCCAAAT TGGCTAACAT TGAAAAAGAA 
TTCAAGAATG ACTATCGCAA GCTGTCTATG CAATGCAAAG ATTTTGTGGT TGGAGTTCTT 
GATCTCTGCC GAGACTCGGA AGAAGTGGAG GCCATCCTTA ATGGGGATTT GAATTCAGAG 
CCGGTTGAAA CGCAGAGACA CAGAGCATCA CTGAGCCGTG TGAAGCTGGC CATTAAGTAT 
GAAGTCAAAA AGTTTGTGGC TCATCCAAAC TGTCAGCAAC AGCTACTGAC TATCTGGTAT 
GAAAATCTCT CAGGACTGCG GGAGCAAACC ATAGCCATCA AGTGTCTGGT TGTGCTGGTT 
GTGGCGTTGG GCCTTCCATT TCTAGCCATC GGTTATTGGA TTGCGCCGTG TAGCAGGCTT 
GGAAGAATTC TTCAAAGCCC ATTTATGAAA TTTGTGGCAC ATGCAGCATC CTTCATCATT 
TTCCTAGGCC TGCTGGTGTT TAATGCTTCT GACAGATTTG AAGGTATAAC AACGCTACCG 
AATGTCACTG TTACCGATTA CCCTAAGCAG ATCTTTAGGG TGAAGACTAC CCAGTTCACC 
TGGACAGAAA TGCTGATCAT GGTATGGGTA CTTGGTATGA TGTGGTCAGA ATGCAAAGAG 
CTGTGGCTGG AAGGACCCAG GGAATATATT TTGCAGTTAT GGAATGTTTT GGATTTTGGG 
ATGTTGTCCA TTTTCATTGC AGCTTTCACA GCGAGGCTTC TAGCATTCCT GCAGGCCACA 
AAAGCACAAC AGTATGTGGA CAACTACATT GAAGAGAGTG ACCTCTCTGA GGTCACGCTT 
CCTCCAGAAA TAGAATATTT TACCTATGCT AGAGATAAAT GGCTTCCATC TGATCCTCAG 
ATAATATCTG AAGGCCTTTA TGCAATTGCT GTTGTTCTTA GCTTTTCTCG GATTGCATAT 
ATCCTGCCTG CGAATGAGAG TTTTGGACCC TTGCAGATTT CTCTTGGAAG GACTGTTAAA 
GACATCTTCA AGTTTATGGT CCTTTTTATT ATGGTATTTC TTGCATTCAT GATTGGAATG 
TTCATACTGT ATTCCTACTA CCTTGGAGCT AAGCTAAACC CAGCCTTTAC AACAGTTGAA 
GAAAGCTTCA AAACCTTATT TTGGTCAATA TTTGGCTTGT CTGAAGTAAC CTCTGTTGTC 
CTGAAATACG ATCATAAGTT CATTGAGAAC ATTGGATATG TTCTCTATGG CATATACAAT 
GTCACGATGG TGGTAGTTCT GCTCAACATG CTGATTGCTA TGATCAACAG CTCATATCAA 
GAAATCGAGG ATGACAGTGA TGTGGAGTGG AAGTTTGCTC GTTCTAAACT TTGGTTATCG 
TATTTTGATG ATGGAAAAAC ATTACCTCCA CCATTTAGTC TTGTACCAAG TCCCAAATCG 
TTTTTCTATT TCATCATGAG GATCATTAAC TTTTCTAAGT GCAGGAGGAG ACGGCTGCAG 
AAGGACATTG AAATGGGAAT GGGCAATTCC AAATCGAGGT TAAACCTTTT CACTCAGTCG 
AACTCCCGAG TTTTTGAATC ACACAGTTTT AACAGCATTC TCAATCAGCC AACACGCTAT 
CAGCAAATAA TGAAAAGACT GATAAAACGT TACGTTCTGA AAGCACAAGT GGACAAAGAA 
AATGATGAAG TAAATGAAGG TGAATTAAAG GAAATCAAAC AAGATATCTC CAGTCTTCGC 
TATGAACTTT TGGAGGACAA GTCCCAGGCG ACAGAAGAGT TAGCCATTTT AATACATAAA 
CTCAGCGAGA AACTCAATCC CAATATGTTA AGATGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244208.1
CDS	1..2802
Translation	MSSKSRKSKEQGRVTFPPQQQQQQQQQQQEEEEEEEAEEEERQRRRRGWRGVNGGLEPPPPPLRAVKTLREPYGYYPPLPFASTMYIEESPSLRRMTMMREKGRRQAIRGPAFMFNNRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYIRIVEAILNHPGFSVNKRLTLSPCEQELQDDDFYSYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCNDCTEKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGDLNSEPVETQRHRASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGLPFLAIGYWIAPCSRLGRILQSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNVTVTDYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARLLAFLQATKAQQYVDNYIEESDLSEVTLPPEIEYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVLFIMVFLAFMIGMFILYSYYLGAKLNPAFTTVEESFKTLFWSIFGLSEVTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLVPSPKSFFYFIMRIINFSKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPNMLRCE

Target ORF information:

RefSeq Version	NM_001257279.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257279.2

ORF Insert Sequence:

ATGTCCAGCA AGAGCCGGAA GAGCAAGGAA CAGGGGAGAG TGACCTTCCC CCCGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAG GAGGAGGAGG AGGAGGAGGC AGAGGAAGAA 
GAGCGGCAGC GCCGGCGCCG CGGCTGGAGA GGCGTCAACG GTGGGCTGGA GCCCCCGCCG 
CCGCCCCTGC GCGCCGTGAA GACCCTCCGG GAGCCCTACG GCTACTACCC GCCGCTGCCC 
TTCGCCAGCA CCATGTACAT TGAAGAAAGT CCGTCTCTGA GGCGTATGAC TATGATGAGG 
GAAAAGGGAA GGCGCCAGGC CATTAGAGGC CCAGCATTCA TGTTCAACAA CAGAGGCACT 
AGTCTGACTG CTGAGGAAGA GCGCTTCCTA GATGCAGCAG AGTATGGGAA CATACCTGTG 
GTGCGCAAAA TGCTGGAGGA GTCAAAAACA CTGAATGTCA ACTGTGTTGA CTACATGGGC 
CAAAACGCCT TGCAGCTTGC TGTAGGGAAC GAACATTTGG AAGTGACAGA ACTTCTGTTA 
AAAAAAGAGA ACTTGGCACG GATTGGAGAT GCCCTGCTGC TCGCAATCAG CAAGGGCTAC 
ATTAGGATAG TGGAAGCAAT TTTAAATCAT CCTGGGTTTT CAGTAAATAA GCGACTGACT 
CTGAGCCCTT GTGAGCAGGA GCTCCAGGAT GACGATTTTT ATTCCTATGA CGAAGACGGC 
ACCCGCTTTT CTCCAGACAT CACTCCCATC ATTTTAGCTG CTCACTGCCA AAAATACGAA 
GTCGTGCATA TGCTGTTGAT GAAAGGAGCA AGGATAGAAA GGCCTCATGA TTATTTCTGC 
AAATGTAATG ACTGTACAGA GAAGCAAAAG CATGATTCTT TCAGTCACTC TCGGTCAAGA 
ATAAATGCCT ACAAAGGACT GGCCAGTCCA GCTTACTTGT CTCTCTCCAG TGAAGACCCT 
GTGCTCACTG CTCTGGAACT CAGCAATGAG CTTGCCAAAT TGGCTAACAT TGAAAAAGAA 
TTCAAGAATG ACTATCGCAA GCTGTCTATG CAATGCAAAG ATTTTGTGGT TGGAGTTCTT 
GATCTCTGCC GAGACTCGGA AGAAGTGGAG GCCATCCTTA ATGGGGATTT GAATTCAGAG 
CCGGTTGAAA CGCAGAGACA CAGAGCATCA CTGAGCCGTG TGAAGCTGGC CATTAAGTAT 
GAAGTCAAAA AGTTTGTGGC TCATCCAAAC TGTCAGCAAC AGCTACTGAC TATCTGGTAT 
GAAAATCTCT CAGGACTGCG GGAGCAAACC ATAGCCATCA AGTGTCTGGT TGTGCTGGTT 
GTGGCGTTGG GCCTTCCATT TCTAGCCATC GGTTATTGGA TTGCGCCGTG TAGCAGGCTT 
GGAAGAATTC TTCAAAGCCC ATTTATGAAA TTTGTGGCAC ATGCAGCATC CTTCATCATT 
TTCCTAGGCC TGCTGGTGTT TAATGCTTCT GACAGATTTG AAGGTATAAC AACGCTACCG 
AATGTCACTG TTACCGATTA CCCTAAGCAG ATCTTTAGGG TGAAGACTAC CCAGTTCACC 
TGGACAGAAA TGCTGATCAT GGTATGGGTA CTTGGTATGA TGTGGTCAGA ATGCAAAGAG 
CTGTGGCTGG AAGGACCCAG GGAATATATT TTGCAGTTAT GGAATGTTTT GGATTTTGGG 
ATGTTGTCCA TTTTCATTGC AGCTTTCACA GCGAGGCTTC TAGCATTCCT GCAGGCCACA 
AAAGCACAAC AGTATGTGGA CAACTACATT GAAGAGAGTG ACCTCTCTGA GGTCACGCTT 
CCTCCAGAAA TAGAATATTT TACCTATGCT AGAGATAAAT GGCTTCCATC TGATCCTCAG 
ATAATATCTG AAGGCCTTTA TGCAATTGCT GTTGTTCTTA GCTTTTCTCG GATTGCATAT 
ATCCTGCCTG CGAATGAGAG TTTTGGACCC TTGCAGATTT CTCTTGGAAG GACTGTTAAA 
GACATCTTCA AGTTTATGGT CCTTTTTATT ATGGTATTTC TTGCATTCAT GATTGGAATG 
TTCATACTGT ATTCCTACTA CCTTGGAGCT AAGCTAAACC CAGCCTTTAC AACAGTTGAA 
GAAAGCTTCA AAACCTTATT TTGGTCAATA TTTGGCTTGT CTGAAGTAAC CTCTGTTGTC 
CTGAAATACG ATCATAAGTT CATTGAGAAC ATTGGATATG TTCTCTATGG CATATACAAT 
GTCACGATGG TGGTAGTTCT GCTCAACATG CTGATTGCTA TGATCAACAG CTCATATCAA 
GAAATCGAGG ATGACAGTGA TGTGGAGTGG AAGTTTGCTC GTTCTAAACT TTGGTTATCG 
TATTTTGATG ATGGAAAAAC ATTACCTCCA CCATTTAGTC TTGTACCAAG TCCCAAATCG 
TTTTTCTATT TCATCATGAG GATCATTAAC TTTTCTAAGT GCAGGAGGAG ACGGCTGCAG 
AAGGACATTG AAATGGGAAT GGGCAATTCC AAATCGAGGT TAAACCTTTT CACTCAGTCG 
AACTCCCGAG TTTTTGAATC ACACAGTTTT AACAGCATTC TCAATCAGCC AACACGCTAT 
CAGCAAATAA TGAAAAGACT GATAAAACGT TACGTTCTGA AAGCACAAGT GGACAAAGAA 
AATGATGAAG TAAATGAAGG TGAATTAAAG GAAATCAAAC AAGATATCTC CAGTCTTCGC 
TATGAACTTT TGGAGGACAA GTCCCAGGCG ACAGAAGAGT TAGCCATTTT AATACATAAA 
CTCAGCGAGA AACTCAATCC CAATATGTTA AGATGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPC3 ( NM_001257279.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001244208.1 Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), mRNA.

Price & Availability↑

CloneID	OGa25071
Clone ID Related Accession (Same CDS sequence)	NM_001257279.3 , NM_001257279.1
Accession Version	NM_001257279.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-25
Organism	Gallus gallus(chicken)
Product	short transient receptor potential channel 3
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AADN05000017.1. On Jun 26, 2018 this sequence version replaced NM_001257279.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201363, SAMEA2201366 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA AGAGCCGGAA GAGCAAGGAA CAGGGGAGAG TGACCTTCCC CCCGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAG GAGGAGGAGG AGGAGGAGGC AGAGGAAGAA 
GAGCGGCAGC GCCGGCGCCG CGGCTGGAGA GGCGTCAACG GTGGGCTGGA GCCCCCGCCG 
CCGCCCCTGC GCGCCGTGAA GACCCTCCGG GAGCCCTACG GCTACTACCC GCCGCTGCCC 
TTCGCCAGCA CCATGTACAT TGAAGAAAGT CCGTCTCTGA GGCGTATGAC TATGATGAGG 
GAAAAGGGAA GGCGCCAGGC CATTAGAGGC CCAGCATTCA TGTTCAACAA CAGAGGCACT 
AGTCTGACTG CTGAGGAAGA GCGCTTCCTA GATGCAGCAG AGTATGGGAA CATACCTGTG 
GTGCGCAAAA TGCTGGAGGA GTCAAAAACA CTGAATGTCA ACTGTGTTGA CTACATGGGC 
CAAAACGCCT TGCAGCTTGC TGTAGGGAAC GAACATTTGG AAGTGACAGA ACTTCTGTTA 
AAAAAAGAGA ACTTGGCACG GATTGGAGAT GCCCTGCTGC TCGCAATCAG CAAGGGCTAC 
ATTAGGATAG TGGAAGCAAT TTTAAATCAT CCTGGGTTTT CAGTAAATAA GCGACTGACT 
CTGAGCCCTT GTGAGCAGGA GCTCCAGGAT GACGATTTTT ATTCCTATGA CGAAGACGGC 
ACCCGCTTTT CTCCAGACAT CACTCCCATC ATTTTAGCTG CTCACTGCCA AAAATACGAA 
GTCGTGCATA TGCTGTTGAT GAAAGGAGCA AGGATAGAAA GGCCTCATGA TTATTTCTGC 
AAATGTAATG ACTGTACAGA GAAGCAAAAG CATGATTCTT TCAGTCACTC TCGGTCAAGA 
ATAAATGCCT ACAAAGGACT GGCCAGTCCA GCTTACCTGT CCCTCTCCAG TGAAGATCCT 
GTGCTCACTG CTCTGGAACT CAGCAATGAG CTTGCCAAAT TGGCTAACAT TGAAAAAGAA 
TTCAAGAATG ACTATCGCAA GCTGTCTATG CAATGCAAAG ATTTTGTGGT TGGAGTTCTT 
GATCTCTGCC GAGACTCGGA AGAAGTGGAG GCCATCCTTA ATGGGGATTT GAATTCAGAG 
CCGGTTGAAA CGCAGAGACA CAGAGCATCA CTGAGCCGTG TGAAGCTGGC CATTAAGTAT 
GAAGTCAAAA AGTTTGTGGC TCATCCAAAC TGTCAGCAAC AGCTACTGAC TATCTGGTAT 
GAAAATCTCT CAGGACTGCG GGAGCAAACC ATAGCCATCA AGTGTCTGGT TGTGCTGGTT 
GTGGCGTTGG GCCTTCCATT TCTAGCCATC GGTTATTGGA TTGCGCCGTG TAGCAGGCTT 
GGAAGAATTC TTCAAAGCCC ATTTATGAAA TTTGTGGCAC ATGCAGCATC CTTCATCATT 
TTCCTAGGCC TGCTGGTGTT TAATGCTTCT GACAGATTTG AAGGTATAAC AACGCTACCG 
AATGTCACTG TTACCGATTA CCCTAAGCAG ATCTTTAGGG TGAAGACTAC CCAGTTCACC 
TGGACAGAAA TGCTGATCAT GGTATGGGTA CTTGGTATGA TGTGGTCAGA ATGCAAAGAG 
CTGTGGCTGG AAGGACCCAG GGAATATATT TTGCAGTTAT GGAATGTTTT GGATTTTGGG 
ATGTTGTCCA TTTTCATTGC AGCTTTCACA GCGAGGCTTC TAGCATTCCT GCAGGCCACA 
AAAGCACAAC AGTATGTGGA CAACTACATT GAAGAGAGTG ACCTCTCTGA GGTCACGCTT 
CCTCCAGAAA TAGAATATTT TACCTATGCT AGAGATAAAT GGCTTCCATC TGATCCTCAG 
ATAATATCTG AAGGCCTTTA TGCAATTGCT GTTGTTCTTA GCTTTTCTCG GATTGCATAT 
ATCCTGCCTG CGAATGAGAG TTTTGGACCC TTGCAGATTT CTCTTGGAAG GACTGTTAAA 
GACATCTTCA AGTTTATGGT CCTTTTTATT ATGGTATTTC TTGCATTCAT GATTGGAATG 
TTCATACTGT ATTCCTACTA CCTTGGAGCT AAGCTAAACC CAGCCTTTAC AACAGTTGAA 
GAAAGCTTCA AAACCTTATT TTGGTCAATA TTTGGCTTGT CTGAAGTAAC CTCTGTTGTC 
CTGAAATACG ATCATAAGTT CATTGAGAAC ATTGGATATG TTCTCTATGG CATATACAAT 
GTCACGATGG TGGTAGTTCT GCTCAACATG CTGATTGCTA TGATCAACAG CTCATATCAA 
GAAATCGAGG ATGACAGTGA TGTGGAGTGG AAGTTTGCTC GTTCTAAACT TTGGTTATCG 
TATTTTGATG ATGGAAAAAC ATTACCTCCA CCATTTAGTC TTGTACCAAG TCCCAAATCG 
TTTTTCTATT TCATCATGAG GATCATTAAC TTTTCTAAGT GCAGGAGGAG ACGGCTGCAG 
AAGGACATTG AAATGGGAAT GGGCAATTCC AAATCGAGGT TAAACCTTTT CACTCAGTCG 
AACTCCCGAG TTTTTGAATC ACACAGTTTT AACAGCATTC TCAATCAGCC AACACGCTAT 
CAGCAAATAA TGAAAAGACT GATAAAACGT TACGTTCTGA AAGCACAAGT GGACAAAGAA 
AATGATGAAG TAAATGAAGG TGAATTAAAG GAAATCAAAC AAGATATCTC CAGTCTTCGC 
TATGAACTTT TGGAGGACAA GTCCCAGGCG ACAGAAGAGT TAGCCATTTT AATACATAAA 
CTCAGCGAGA AACTCAATCC CAATATGTTG AGATGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244208.1
CDS	1..2802
Translation	MSSKSRKSKEQGRVTFPPQQQQQQQQQQQEEEEEEEAEEEERQRRRRGWRGVNGGLEPPPPPLRAVKTLREPYGYYPPLPFASTMYIEESPSLRRMTMMREKGRRQAIRGPAFMFNNRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYIRIVEAILNHPGFSVNKRLTLSPCEQELQDDDFYSYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCNDCTEKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGDLNSEPVETQRHRASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGLPFLAIGYWIAPCSRLGRILQSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNVTVTDYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARLLAFLQATKAQQYVDNYIEESDLSEVTLPPEIEYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVLFIMVFLAFMIGMFILYSYYLGAKLNPAFTTVEESFKTLFWSIFGLSEVTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLVPSPKSFFYFIMRIINFSKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPNMLRCE

Target ORF information:

RefSeq Version	NM_001257279.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257279.3

ORF Insert Sequence:

ATGTCCAGCA AGAGCCGGAA GAGCAAGGAA CAGGGGAGAG TGACCTTCCC CCCGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAG GAGGAGGAGG AGGAGGAGGC AGAGGAAGAA 
GAGCGGCAGC GCCGGCGCCG CGGCTGGAGA GGCGTCAACG GTGGGCTGGA GCCCCCGCCG 
CCGCCCCTGC GCGCCGTGAA GACCCTCCGG GAGCCCTACG GCTACTACCC GCCGCTGCCC 
TTCGCCAGCA CCATGTACAT TGAAGAAAGT CCGTCTCTGA GGCGTATGAC TATGATGAGG 
GAAAAGGGAA GGCGCCAGGC CATTAGAGGC CCAGCATTCA TGTTCAACAA CAGAGGCACT 
AGTCTGACTG CTGAGGAAGA GCGCTTCCTA GATGCAGCAG AGTATGGGAA CATACCTGTG 
GTGCGCAAAA TGCTGGAGGA GTCAAAAACA CTGAATGTCA ACTGTGTTGA CTACATGGGC 
CAAAACGCCT TGCAGCTTGC TGTAGGGAAC GAACATTTGG AAGTGACAGA ACTTCTGTTA 
AAAAAAGAGA ACTTGGCACG GATTGGAGAT GCCCTGCTGC TCGCAATCAG CAAGGGCTAC 
ATTAGGATAG TGGAAGCAAT TTTAAATCAT CCTGGGTTTT CAGTAAATAA GCGACTGACT 
CTGAGCCCTT GTGAGCAGGA GCTCCAGGAT GACGATTTTT ATTCCTATGA CGAAGACGGC 
ACCCGCTTTT CTCCAGACAT CACTCCCATC ATTTTAGCTG CTCACTGCCA AAAATACGAA 
GTCGTGCATA TGCTGTTGAT GAAAGGAGCA AGGATAGAAA GGCCTCATGA TTATTTCTGC 
AAATGTAATG ACTGTACAGA GAAGCAAAAG CATGATTCTT TCAGTCACTC TCGGTCAAGA 
ATAAATGCCT ACAAAGGACT GGCCAGTCCA GCTTACCTGT CCCTCTCCAG TGAAGATCCT 
GTGCTCACTG CTCTGGAACT CAGCAATGAG CTTGCCAAAT TGGCTAACAT TGAAAAAGAA 
TTCAAGAATG ACTATCGCAA GCTGTCTATG CAATGCAAAG ATTTTGTGGT TGGAGTTCTT 
GATCTCTGCC GAGACTCGGA AGAAGTGGAG GCCATCCTTA ATGGGGATTT GAATTCAGAG 
CCGGTTGAAA CGCAGAGACA CAGAGCATCA CTGAGCCGTG TGAAGCTGGC CATTAAGTAT 
GAAGTCAAAA AGTTTGTGGC TCATCCAAAC TGTCAGCAAC AGCTACTGAC TATCTGGTAT 
GAAAATCTCT CAGGACTGCG GGAGCAAACC ATAGCCATCA AGTGTCTGGT TGTGCTGGTT 
GTGGCGTTGG GCCTTCCATT TCTAGCCATC GGTTATTGGA TTGCGCCGTG TAGCAGGCTT 
GGAAGAATTC TTCAAAGCCC ATTTATGAAA TTTGTGGCAC ATGCAGCATC CTTCATCATT 
TTCCTAGGCC TGCTGGTGTT TAATGCTTCT GACAGATTTG AAGGTATAAC AACGCTACCG 
AATGTCACTG TTACCGATTA CCCTAAGCAG ATCTTTAGGG TGAAGACTAC CCAGTTCACC 
TGGACAGAAA TGCTGATCAT GGTATGGGTA CTTGGTATGA TGTGGTCAGA ATGCAAAGAG 
CTGTGGCTGG AAGGACCCAG GGAATATATT TTGCAGTTAT GGAATGTTTT GGATTTTGGG 
ATGTTGTCCA TTTTCATTGC AGCTTTCACA GCGAGGCTTC TAGCATTCCT GCAGGCCACA 
AAAGCACAAC AGTATGTGGA CAACTACATT GAAGAGAGTG ACCTCTCTGA GGTCACGCTT 
CCTCCAGAAA TAGAATATTT TACCTATGCT AGAGATAAAT GGCTTCCATC TGATCCTCAG 
ATAATATCTG AAGGCCTTTA TGCAATTGCT GTTGTTCTTA GCTTTTCTCG GATTGCATAT 
ATCCTGCCTG CGAATGAGAG TTTTGGACCC TTGCAGATTT CTCTTGGAAG GACTGTTAAA 
GACATCTTCA AGTTTATGGT CCTTTTTATT ATGGTATTTC TTGCATTCAT GATTGGAATG 
TTCATACTGT ATTCCTACTA CCTTGGAGCT AAGCTAAACC CAGCCTTTAC AACAGTTGAA 
GAAAGCTTCA AAACCTTATT TTGGTCAATA TTTGGCTTGT CTGAAGTAAC CTCTGTTGTC 
CTGAAATACG ATCATAAGTT CATTGAGAAC ATTGGATATG TTCTCTATGG CATATACAAT 
GTCACGATGG TGGTAGTTCT GCTCAACATG CTGATTGCTA TGATCAACAG CTCATATCAA 
GAAATCGAGG ATGACAGTGA TGTGGAGTGG AAGTTTGCTC GTTCTAAACT TTGGTTATCG 
TATTTTGATG ATGGAAAAAC ATTACCTCCA CCATTTAGTC TTGTACCAAG TCCCAAATCG 
TTTTTCTATT TCATCATGAG GATCATTAAC TTTTCTAAGT GCAGGAGGAG ACGGCTGCAG 
AAGGACATTG AAATGGGAAT GGGCAATTCC AAATCGAGGT TAAACCTTTT CACTCAGTCG 
AACTCCCGAG TTTTTGAATC ACACAGTTTT AACAGCATTC TCAATCAGCC AACACGCTAT 
CAGCAAATAA TGAAAAGACT GATAAAACGT TACGTTCTGA AAGCACAAGT GGACAAAGAA 
AATGATGAAG TAAATGAAGG TGAATTAAAG GAAATCAAAC AAGATATCTC CAGTCTTCGC 
TATGAACTTT TGGAGGACAA GTCCCAGGCG ACAGAAGAGT TAGCCATTTT AATACATAAA 
CTCAGCGAGA AACTCAATCC CAATATGTTG AGATGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPC3 ( NM_001257279.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001244208.1 Gallus gallus transient receptor potential cation channel, subfamily C, member 3 (TRPC3), mRNA.

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CloneID	OGa25071
Clone ID Related Accession (Same CDS sequence)	NM_001257279.3 , NM_001257279.1
Accession Version	NM_001257279.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-08-26
Organism	Gallus gallus(Chicken)
Product	short transient receptor potential channel 3
Comment	INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AADN03004278.1. On Mar 28, 2012 this sequence version replaced XM_003641203.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SRS213963, SRS213964 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA AGAGCCGGAA GAGCAAGGAA CAGGGGAGAG TGACCTTCCC CCCGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAG GAGGAGGAGG AGGAGGAGGC AGAGGAAGAA 
GAGCGGCAGC GCCGGCGCCG CGGCTGGAGA GGCGTCAACG GTGGGCTGGA GCCCCCGCCG 
CCGCCCCTGC GCGCCGTGAA GACCCTCCGG GAGCCCTACG GCTACTACCC GCCGCTGCCC 
TTCGCCAGCA CCATGTACAT TGAAGAAAGT CCGTCTCTGA GGCGTATGAC TATGATGAGG 
GAAAAGGGAA GGCGCCAGGC CATTAGAGGC CCAGCATTCA TGTTCAACAA CAGAGGCACT 
AGTCTGACTG CTGAGGAAGA GCGCTTCCTA GATGCAGCAG AGTATGGGAA CATACCTGTG 
GTGCGCAAAA TGCTGGAGGA GTCAAAAACA CTGAATGTCA ACTGTGTTGA CTACATGGGC 
CAAAACGCCT TGCAGCTTGC TGTAGGGAAC GAACATTTGG AAGTGACAGA ACTTCTGTTA 
AAAAAAGAGA ACTTGGCACG GATTGGAGAT GCCCTGCTGC TCGCAATCAG CAAGGGCTAC 
ATTAGGATAG TGGAAGCAAT TTTAAATCAT CCTGGGTTTT CAGTAAATAA GCGACTGACT 
CTGAGCCCTT GTGAGCAGGA GCTCCAGGAT GACGATTTTT ATTCCTATGA CGAAGACGGC 
ACCCGCTTTT CTCCAGACAT CACTCCCATC ATTTTAGCTG CTCACTGCCA AAAATACGAA 
GTCGTGCATA TGCTGTTGAT GAAAGGAGCA AGGATAGAAA GGCCTCATGA TTATTTCTGC 
AAATGTAATG ACTGTACAGA GAAGCAAAAG CATGATTCTT TCAGTCACTC TCGGTCAAGA 
ATAAATGCCT ACAAAGGACT GGCCAGTCCA GCTTACCTGT CCCTCTCCAG TGAAGATCCT 
GTGCTCACTG CTCTGGAACT CAGCAATGAG CTTGCCAAAT TGGCTAACAT TGAAAAAGAA 
TTCAAGAATG ACTATCGCAA GCTGTCTATG CAATGCAAAG ATTTTGTGGT TGGAGTTCTT 
GATCTCTGCC GAGACTCGGA AGAAGTGGAG GCCATCCTTA ATGGGGATTT GAATTCAGAG 
CCGGTTGAAA CGCAGAGACA CAGAGCATCA CTGAGCCGTG TGAAGCTGGC CATTAAGTAT 
GAAGTCAAAA AGTTTGTGGC TCATCCAAAC TGTCAGCAAC AGCTACTGAC TATCTGGTAT 
GAAAATCTCT CAGGACTGCG GGAGCAAACC ATAGCCATCA AGTGTCTGGT TGTGCTGGTT 
GTGGCGTTGG GCCTTCCATT TCTAGCCATC GGTTATTGGA TTGCGCCGTG TAGCAGGCTT 
GGAAGAATTC TTCAAAGCCC ATTTATGAAA TTTGTGGCAC ATGCAGCATC CTTCATCATT 
TTCCTAGGCC TGCTGGTGTT TAATGCTTCT GACAGATTTG AAGGTATAAC AACGCTACCG 
AATGTCACTG TTACCGATTA CCCTAAGCAG ATCTTTAGGG TGAAGACTAC CCAGTTCACC 
TGGACAGAAA TGCTGATCAT GGTATGGGTA CTTGGTATGA TGTGGTCAGA ATGCAAAGAG 
CTGTGGCTGG AAGGACCCAG GGAATATATT TTGCAGTTAT GGAATGTTTT GGATTTTGGG 
ATGTTGTCCA TTTTCATTGC AGCTTTCACA GCGAGGCTTC TAGCATTCCT GCAGGCCACA 
AAAGCACAAC AGTATGTGGA CAACTACATT GAAGAGAGTG ACCTCTCTGA GGTCACGCTT 
CCTCCAGAAA TAGAATATTT TACCTATGCT AGAGATAAAT GGCTTCCATC TGATCCTCAG 
ATAATATCTG AAGGCCTTTA TGCAATTGCT GTTGTTCTTA GCTTTTCTCG GATTGCATAT 
ATCCTGCCTG CGAATGAGAG TTTTGGACCC TTGCAGATTT CTCTTGGAAG GACTGTTAAA 
GACATCTTCA AGTTTATGGT CCTTTTTATT ATGGTATTTC TTGCATTCAT GATTGGAATG 
TTCATACTGT ATTCCTACTA CCTTGGAGCT AAGCTAAACC CAGCCTTTAC AACAGTTGAA 
GAAAGCTTCA AAACCTTATT TTGGTCAATA TTTGGCTTGT CTGAAGTAAC CTCTGTTGTC 
CTGAAATACG ATCATAAGTT CATTGAGAAC ATTGGATATG TTCTCTATGG CATATACAAT 
GTCACGATGG TGGTAGTTCT GCTCAACATG CTGATTGCTA TGATCAACAG CTCATATCAA 
GAAATCGAGG ATGACAGTGA TGTGGAGTGG AAGTTTGCTC GTTCTAAACT TTGGTTATCG 
TATTTTGATG ATGGAAAAAC ATTACCTCCA CCATTTAGTC TTGTACCAAG TCCCAAATCG 
TTTTTCTATT TCATCATGAG GATCATTAAC TTTTCTAAGT GCAGGAGGAG ACGGCTGCAG 
AAGGACATTG AAATGGGAAT GGGCAATTCC AAATCGAGGT TAAACCTTTT CACTCAGTCG 
AACTCCCGAG TTTTTGAATC ACACAGTTTT AACAGCATTC TCAATCAGCC AACACGCTAT 
CAGCAAATAA TGAAAAGACT GATAAAACGT TACGTTCTGA AAGCACAAGT GGACAAAGAA 
AATGATGAAG TAAATGAAGG TGAATTAAAG GAAATCAAAC AAGATATCTC CAGTCTTCGC 
TATGAACTTT TGGAGGACAA GTCCCAGGCG ACAGAAGAGT TAGCCATTTT AATACATAAA 
CTCAGCGAGA AACTCAATCC CAATATGTTG AGATGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244208.1
CDS	1..2802
Translation	MSSKSRKSKEQGRVTFPPQQQQQQQQQQQEEEEEEEAEEEERQRRRRGWRGVNGGLEPPPPPLRAVKTLREPYGYYPPLPFASTMYIEESPSLRRMTMMREKGRRQAIRGPAFMFNNRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYIRIVEAILNHPGFSVNKRLTLSPCEQELQDDDFYSYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCNDCTEKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGDLNSEPVETQRHRASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGLPFLAIGYWIAPCSRLGRILQSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNVTVTDYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARLLAFLQATKAQQYVDNYIEESDLSEVTLPPEIEYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVLFIMVFLAFMIGMFILYSYYLGAKLNPAFTTVEESFKTLFWSIFGLSEVTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLVPSPKSFFYFIMRIINFSKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPNMLRCE

Target ORF information:

RefSeq Version	NM_001257279.1
Organism	Gallus gallus(Chicken)
Definition	Gallus gallus transient receptor potential cation channel, subfamily C, member 3 (TRPC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257279.1

ORF Insert Sequence:

ATGTCCAGCA AGAGCCGGAA GAGCAAGGAA CAGGGGAGAG TGACCTTCCC CCCGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAG GAGGAGGAGG AGGAGGAGGC AGAGGAAGAA 
GAGCGGCAGC GCCGGCGCCG CGGCTGGAGA GGCGTCAACG GTGGGCTGGA GCCCCCGCCG 
CCGCCCCTGC GCGCCGTGAA GACCCTCCGG GAGCCCTACG GCTACTACCC GCCGCTGCCC 
TTCGCCAGCA CCATGTACAT TGAAGAAAGT CCGTCTCTGA GGCGTATGAC TATGATGAGG 
GAAAAGGGAA GGCGCCAGGC CATTAGAGGC CCAGCATTCA TGTTCAACAA CAGAGGCACT 
AGTCTGACTG CTGAGGAAGA GCGCTTCCTA GATGCAGCAG AGTATGGGAA CATACCTGTG 
GTGCGCAAAA TGCTGGAGGA GTCAAAAACA CTGAATGTCA ACTGTGTTGA CTACATGGGC 
CAAAACGCCT TGCAGCTTGC TGTAGGGAAC GAACATTTGG AAGTGACAGA ACTTCTGTTA 
AAAAAAGAGA ACTTGGCACG GATTGGAGAT GCCCTGCTGC TCGCAATCAG CAAGGGCTAC 
ATTAGGATAG TGGAAGCAAT TTTAAATCAT CCTGGGTTTT CAGTAAATAA GCGACTGACT 
CTGAGCCCTT GTGAGCAGGA GCTCCAGGAT GACGATTTTT ATTCCTATGA CGAAGACGGC 
ACCCGCTTTT CTCCAGACAT CACTCCCATC ATTTTAGCTG CTCACTGCCA AAAATACGAA 
GTCGTGCATA TGCTGTTGAT GAAAGGAGCA AGGATAGAAA GGCCTCATGA TTATTTCTGC 
AAATGTAATG ACTGTACAGA GAAGCAAAAG CATGATTCTT TCAGTCACTC TCGGTCAAGA 
ATAAATGCCT ACAAAGGACT GGCCAGTCCA GCTTACCTGT CCCTCTCCAG TGAAGATCCT 
GTGCTCACTG CTCTGGAACT CAGCAATGAG CTTGCCAAAT TGGCTAACAT TGAAAAAGAA 
TTCAAGAATG ACTATCGCAA GCTGTCTATG CAATGCAAAG ATTTTGTGGT TGGAGTTCTT 
GATCTCTGCC GAGACTCGGA AGAAGTGGAG GCCATCCTTA ATGGGGATTT GAATTCAGAG 
CCGGTTGAAA CGCAGAGACA CAGAGCATCA CTGAGCCGTG TGAAGCTGGC CATTAAGTAT 
GAAGTCAAAA AGTTTGTGGC TCATCCAAAC TGTCAGCAAC AGCTACTGAC TATCTGGTAT 
GAAAATCTCT CAGGACTGCG GGAGCAAACC ATAGCCATCA AGTGTCTGGT TGTGCTGGTT 
GTGGCGTTGG GCCTTCCATT TCTAGCCATC GGTTATTGGA TTGCGCCGTG TAGCAGGCTT 
GGAAGAATTC TTCAAAGCCC ATTTATGAAA TTTGTGGCAC ATGCAGCATC CTTCATCATT 
TTCCTAGGCC TGCTGGTGTT TAATGCTTCT GACAGATTTG AAGGTATAAC AACGCTACCG 
AATGTCACTG TTACCGATTA CCCTAAGCAG ATCTTTAGGG TGAAGACTAC CCAGTTCACC 
TGGACAGAAA TGCTGATCAT GGTATGGGTA CTTGGTATGA TGTGGTCAGA ATGCAAAGAG 
CTGTGGCTGG AAGGACCCAG GGAATATATT TTGCAGTTAT GGAATGTTTT GGATTTTGGG 
ATGTTGTCCA TTTTCATTGC AGCTTTCACA GCGAGGCTTC TAGCATTCCT GCAGGCCACA 
AAAGCACAAC AGTATGTGGA CAACTACATT GAAGAGAGTG ACCTCTCTGA GGTCACGCTT 
CCTCCAGAAA TAGAATATTT TACCTATGCT AGAGATAAAT GGCTTCCATC TGATCCTCAG 
ATAATATCTG AAGGCCTTTA TGCAATTGCT GTTGTTCTTA GCTTTTCTCG GATTGCATAT 
ATCCTGCCTG CGAATGAGAG TTTTGGACCC TTGCAGATTT CTCTTGGAAG GACTGTTAAA 
GACATCTTCA AGTTTATGGT CCTTTTTATT ATGGTATTTC TTGCATTCAT GATTGGAATG 
TTCATACTGT ATTCCTACTA CCTTGGAGCT AAGCTAAACC CAGCCTTTAC AACAGTTGAA 
GAAAGCTTCA AAACCTTATT TTGGTCAATA TTTGGCTTGT CTGAAGTAAC CTCTGTTGTC 
CTGAAATACG ATCATAAGTT CATTGAGAAC ATTGGATATG TTCTCTATGG CATATACAAT 
GTCACGATGG TGGTAGTTCT GCTCAACATG CTGATTGCTA TGATCAACAG CTCATATCAA 
GAAATCGAGG ATGACAGTGA TGTGGAGTGG AAGTTTGCTC GTTCTAAACT TTGGTTATCG 
TATTTTGATG ATGGAAAAAC ATTACCTCCA CCATTTAGTC TTGTACCAAG TCCCAAATCG 
TTTTTCTATT TCATCATGAG GATCATTAAC TTTTCTAAGT GCAGGAGGAG ACGGCTGCAG 
AAGGACATTG AAATGGGAAT GGGCAATTCC AAATCGAGGT TAAACCTTTT CACTCAGTCG 
AACTCCCGAG TTTTTGAATC ACACAGTTTT AACAGCATTC TCAATCAGCC AACACGCTAT 
CAGCAAATAA TGAAAAGACT GATAAAACGT TACGTTCTGA AAGCACAAGT GGACAAAGAA 
AATGATGAAG TAAATGAAGG TGAATTAAAG GAAATCAAAC AAGATATCTC CAGTCTTCGC 
TATGAACTTT TGGAGGACAA GTCCCAGGCG ACAGAAGAGT TAGCCATTTT AATACATAAA 
CTCAGCGAGA AACTCAATCC CAATATGTTG AGATGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPC3 ( XM_015276103.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131589.1 Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa149990
Clone ID Related Accession (Same CDS sequence)	XM_015276103.2
Accession Version	XM_015276103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	short transient receptor potential channel 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATGA TGAGGGAAAA GGGAAGGCGC CAGGCCATTA GAGGCCCAGC ATTCATGTTC 
AACAACAGAG GCACTAGTCT GACTGCTGAG GAAGAGCGCT TCCTAGATGC AGCAGAGTAT 
GGGAACATAC CTGTGGTGCG CAAAATGCTG GAGGAGTCAA AAACACTGAA TGTCAACTGT 
GTTGACTACA TGGGCCAAAA CGCCTTGCAG CTTGCTGTAG GGAACGAACA TTTGGAAGTG 
ACAGAACTTC TGTTAAAAAA AGAGAACTTG GCACGGATTG GAGATGCCCT GCTGCTCGCA 
ATCAGCAAGG GCTACATTAG GATAGTGGAA GCAATTTTAA ATCATCCTGG GTTTTCAGTA 
AATAAGCGAC TGACTCTGAG CCCTTGTGAG CAGGAGCTCC AGGATGACGA TTTTTATTCC 
TATGACGAAG ACGGCACCCG CTTTTCTCCA GACATCACTC CCATCATTTT AGCTGCTCAC 
TGCCAAAAAT ACGAAGTCGT GCATATGCTG TTGATGAAAG GAGCAAGGAT AGAAAGGCCT 
CATGATTATT TCTGCAAATG TAATGACTGT ACAGAGAAGC AAAAGCATGA TTCTTTCAGT 
CACTCTCGGT CAAGAATAAA TGCCTACAAA GGACTGGCCA GTCCAGCTTA CCTGTCCCTC 
TCCAGTGAAG ATCCTGTGCT CACTGCTCTG GAACTCAGCA ATGAGCTTGC CAAATTGGCT 
AACATTGAAA AAGAATTCAA GAATGACTAT CGCAAGCTGT CTATGCAATG CAAAGATTTT 
GTGGTTGGAG TTCTTGATCT CTGCCGAGAC TCGGAAGAAG TGGAGGCCAT CCTTAATGGG 
GATTTGAATT CAGAGCCGGT TGAAACGCAG AGACACAGAG CATCACTGAG CCGTGTGAAG 
CTGGCCATTA AGTATGAAGT CAAAAAGTTT GTGGCTCATC CAAACTGTCA GCAACAGCTA 
CTGACTATCT GGTATGAAAA TCTCTCAGGA CTGCGGGAGC AAACCATAGC CATCAAGTGT 
CTGGTTGTGC TGGTTGTGGC GTTGGGCCTT CCATTTCTAG CCATCGGTTA TTGGATTGCG 
CCGTGTAGCA GGCTTGGAAG AATTCTTCAA AGCCCATTTA TGAAATTTGT GGCACATGCA 
GCATCCTTCA TCATTTTCCT AGGCCTGCTG GTGTTTAATG CTTCTGACAG ATTTGAAGGT 
ATAACAACGC TACCGAATGT CACTGTTACC GATTACCCTA AGCAGATCTT TAGGGTGAAG 
ACTACCCAGT TCACCTGGAC AGAAATGCTG ATCATGGTAT GGGTACTTGG TATGATGTGG 
TCAGAATGCA AAGAGCTGTG GCTGGAAGGA CCCAGGGAAT ATATTTTGCA GTTATGGAAT 
GTTTTGGATT TTGGGATGTT GTCCATTTTC ATTGCAGCTT TCACAGCGAG GCTTCTAGCA 
TTCCTGCAGG CCACAAAAGC ACAACAGTAT GTGGACAACT ACATTGAAGA GAGTGACCTC 
TCTGAGGTCA CGCTTCCTCC AGAAATAGAA TATTTTACCT ATGCTAGAGA TAAATGGCTT 
CCATCTGATC CTCAGATAAT ATCTGAAGGC CTTTATGCAA TTGCTGTTGT TCTTAGCTTT 
TCTCGGATTG CATATATCCT GCCTGCGAAT GAGAGTTTTG GACCCTTGCA GATTTCTCTT 
GGAAGGACTG TTAAAGACAT CTTCAAGTTT ATGGTCCTTT TTATTATGGT ATTTCTTGCA 
TTCATGATTG GAATGTTCAT ACTGTATTCC TACTACCTTG GAGCTAAGCT AAACCCAGCC 
TTTACAACAG TTGAAGAAAG CTTCAAAACC TTATTTTGGT CAATATTTGG CTTGTCTGAA 
GTAACCTCTG TTGTCCTGAA ATACGATCAT AAGTTCATTG AGAACATTGG ATATGTTCTC 
TATGGCATAT ACAATGTCAC GATGGTGGTA GTTCTGCTCA ACATGCTGAT TGCTATGATC 
AACAGCTCAT ATCAAGAAAT CGAGGATGAC AGTGATGTGG AGTGGAAGTT TGCTCGTTCT 
AAACTTTGGT TATCGTATTT TGATGATGGA AAAACATTAC CTCCACCATT TAGTCTTGTA 
CCAAGTCCCA AATCGTTTTT CTATTTCATC ATGAGGATCA TTAACTTTTC TAAGTGCAGG 
AGGAGACGGC TGCAGAAGGA CATTGAAATG GGAATGGGCA ATTCCAAATC GAGGTTAAAC 
CTTTTCACTC AGTCGAACTC CCGAGTTTTT GAATCACACA GTTTTAACAG CATTCTCAAT 
CAGCCAACAC GCTATCAGCA AATAATGAAA AGACTGATAA AACGTTACGT TCTGAAAGCA 
CAAGTGGACA AAGAAAATGA TGAAGTAAAT GAAGGTGAAT TAAAGGAAAT CAAACAAGAT 
ATCTCCAGTC TTCGCTATGA ACTTTTGGAG GACAAGTCCC AGGCGACAGA AGAGTTAGCC 
ATTTTAATAC ATAAACTCAG CGAGAAACTC AATCCCAATA TGTTGAGATG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131589.1
CDS	321..2837
Translation	MTMMREKGRRQAIRGPAFMFNNRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYIRIVEAILNHPGFSVNKRLTLSPCEQELQDDDFYSYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCNDCTEKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGDLNSEPVETQRHRASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGLPFLAIGYWIAPCSRLGRILQSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNVTVTDYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARLLAFLQATKAQQYVDNYIEESDLSEVTLPPEIEYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVLFIMVFLAFMIGMFILYSYYLGAKLNPAFTTVEESFKTLFWSIFGLSEVTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLVPSPKSFFYFIMRIINFSKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPNMLRCE

Target ORF information:

RefSeq Version	XM_015276103.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus transient receptor potential cation channel subfamily C member 3 (TRPC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276103.2

ORF Insert Sequence:

ATGACTATGA TGAGGGAAAA GGGAAGGCGC CAGGCCATTA GAGGCCCAGC ATTCATGTTC 
AACAACAGAG GCACTAGTCT GACTGCTGAG GAAGAGCGCT TCCTAGATGC AGCAGAGTAT 
GGGAACATAC CTGTGGTGCG CAAAATGCTG GAGGAGTCAA AAACACTGAA TGTCAACTGT 
GTTGACTACA TGGGCCAAAA CGCCTTGCAG CTTGCTGTAG GGAACGAACA TTTGGAAGTG 
ACAGAACTTC TGTTAAAAAA AGAGAACTTG GCACGGATTG GAGATGCCCT GCTGCTCGCA 
ATCAGCAAGG GCTACATTAG GATAGTGGAA GCAATTTTAA ATCATCCTGG GTTTTCAGTA 
AATAAGCGAC TGACTCTGAG CCCTTGTGAG CAGGAGCTCC AGGATGACGA TTTTTATTCC 
TATGACGAAG ACGGCACCCG CTTTTCTCCA GACATCACTC CCATCATTTT AGCTGCTCAC 
TGCCAAAAAT ACGAAGTCGT GCATATGCTG TTGATGAAAG GAGCAAGGAT AGAAAGGCCT 
CATGATTATT TCTGCAAATG TAATGACTGT ACAGAGAAGC AAAAGCATGA TTCTTTCAGT 
CACTCTCGGT CAAGAATAAA TGCCTACAAA GGACTGGCCA GTCCAGCTTA CCTGTCCCTC 
TCCAGTGAAG ATCCTGTGCT CACTGCTCTG GAACTCAGCA ATGAGCTTGC CAAATTGGCT 
AACATTGAAA AAGAATTCAA GAATGACTAT CGCAAGCTGT CTATGCAATG CAAAGATTTT 
GTGGTTGGAG TTCTTGATCT CTGCCGAGAC TCGGAAGAAG TGGAGGCCAT CCTTAATGGG 
GATTTGAATT CAGAGCCGGT TGAAACGCAG AGACACAGAG CATCACTGAG CCGTGTGAAG 
CTGGCCATTA AGTATGAAGT CAAAAAGTTT GTGGCTCATC CAAACTGTCA GCAACAGCTA 
CTGACTATCT GGTATGAAAA TCTCTCAGGA CTGCGGGAGC AAACCATAGC CATCAAGTGT 
CTGGTTGTGC TGGTTGTGGC GTTGGGCCTT CCATTTCTAG CCATCGGTTA TTGGATTGCG 
CCGTGTAGCA GGCTTGGAAG AATTCTTCAA AGCCCATTTA TGAAATTTGT GGCACATGCA 
GCATCCTTCA TCATTTTCCT AGGCCTGCTG GTGTTTAATG CTTCTGACAG ATTTGAAGGT 
ATAACAACGC TACCGAATGT CACTGTTACC GATTACCCTA AGCAGATCTT TAGGGTGAAG 
ACTACCCAGT TCACCTGGAC AGAAATGCTG ATCATGGTAT GGGTACTTGG TATGATGTGG 
TCAGAATGCA AAGAGCTGTG GCTGGAAGGA CCCAGGGAAT ATATTTTGCA GTTATGGAAT 
GTTTTGGATT TTGGGATGTT GTCCATTTTC ATTGCAGCTT TCACAGCGAG GCTTCTAGCA 
TTCCTGCAGG CCACAAAAGC ACAACAGTAT GTGGACAACT ACATTGAAGA GAGTGACCTC 
TCTGAGGTCA CGCTTCCTCC AGAAATAGAA TATTTTACCT ATGCTAGAGA TAAATGGCTT 
CCATCTGATC CTCAGATAAT ATCTGAAGGC CTTTATGCAA TTGCTGTTGT TCTTAGCTTT 
TCTCGGATTG CATATATCCT GCCTGCGAAT GAGAGTTTTG GACCCTTGCA GATTTCTCTT 
GGAAGGACTG TTAAAGACAT CTTCAAGTTT ATGGTCCTTT TTATTATGGT ATTTCTTGCA 
TTCATGATTG GAATGTTCAT ACTGTATTCC TACTACCTTG GAGCTAAGCT AAACCCAGCC 
TTTACAACAG TTGAAGAAAG CTTCAAAACC TTATTTTGGT CAATATTTGG CTTGTCTGAA 
GTAACCTCTG TTGTCCTGAA ATACGATCAT AAGTTCATTG AGAACATTGG ATATGTTCTC 
TATGGCATAT ACAATGTCAC GATGGTGGTA GTTCTGCTCA ACATGCTGAT TGCTATGATC 
AACAGCTCAT ATCAAGAAAT CGAGGATGAC AGTGATGTGG AGTGGAAGTT TGCTCGTTCT 
AAACTTTGGT TATCGTATTT TGATGATGGA AAAACATTAC CTCCACCATT TAGTCTTGTA 
CCAAGTCCCA AATCGTTTTT CTATTTCATC ATGAGGATCA TTAACTTTTC TAAGTGCAGG 
AGGAGACGGC TGCAGAAGGA CATTGAAATG GGAATGGGCA ATTCCAAATC GAGGTTAAAC 
CTTTTCACTC AGTCGAACTC CCGAGTTTTT GAATCACACA GTTTTAACAG CATTCTCAAT 
CAGCCAACAC GCTATCAGCA AATAATGAAA AGACTGATAA AACGTTACGT TCTGAAAGCA 
CAAGTGGACA AAGAAAATGA TGAAGTAAAT GAAGGTGAAT TAAAGGAAAT CAAACAAGAT 
ATCTCCAGTC TTCGCTATGA ACTTTTGGAG GACAAGTCCC AGGCGACAGA AGAGTTAGCC 
ATTTTAATAC ATAAACTCAG CGAGAAACTC AATCCCAATA TGTTGAGATG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPC3 ( XM_015276103.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131589.1 Gallus gallus transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant X1, mRNA.

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CloneID	OGa35000
Clone ID Related Accession (Same CDS sequence)	XM_015276103.1
Accession Version	XM_015276103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	short transient receptor potential channel 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATGA TGAGGGAAAA GGGAAGGCGC CAGGCCATTA GAGGCCCAGC ATTCATGTTC 
AACAACAGAG GCACTAGTCT GACTGCTGAG GAAGAGCGCT TCCTAGATGC AGCAGAGTAT 
GGGAACATAC CTGTGGTGCG CAAAATGCTG GAGGAGTCAA AAACACTGAA TGTCAACTGT 
GTTGACTACA TGGGCCAAAA CGCCTTGCAG CTTGCTGTAG GGAACGAACA TTTGGAAGTG 
ACAGAACTTC TGTTAAAAAA AGAGAACTTG GCACGGATTG GAGATGCCCT GCTGCTCGCA 
ATCAGCAAGG GCTACATTAG GATAGTGGAA GCAATTTTAA ATCATCCTGG GTTTTCAGTA 
AATAAGCGAC TGACTCTGAG CCCTTGTGAG CAGGAGCTCC AGGATGACGA TTTTTATTCC 
TATGACGAAG ACGGCACCCG CTTTTCTCCA GACATCACTC CCATCATTTT AGCTGCTCAC 
TGCCAAAAAT ACGAAGTCGT GCATATGCTG TTGATGAAAG GAGCAAGGAT AGAAAGGCCT 
CATGATTATT TCTGCAAATG TAATGACTGT ACAGAGAAGC AAAAGCATGA TTCTTTCAGT 
CACTCTCGGT CAAGAATAAA TGCCTACAAA GGACTGGCCA GTCCAGCTTA CTTGTCTCTC 
TCCAGTGAAG ACCCTGTGCT CACTGCTCTG GAACTCAGCA ATGAGCTTGC CAAATTGGCT 
AACATTGAAA AAGAATTCAA GAATGACTAT CGCAAGCTGT CTATGCAATG CAAAGATTTT 
GTGGTTGGAG TTCTTGATCT CTGCCGAGAC TCGGAAGAAG TGGAGGCCAT CCTTAATGGG 
GATTTGAATT CAGAGCCGGT TGAAACGCAG AGACACAGAG CATCACTGAG CCGTGTGAAG 
CTGGCCATTA AGTATGAAGT CAAAAAGTTT GTGGCTCATC CAAACTGTCA GCAACAGCTA 
CTGACTATCT GGTATGAAAA TCTCTCAGGA CTGCGGGAGC AAACCATAGC CATCAAGTGT 
CTGGTTGTGC TGGTTGTGGC GTTGGGCCTT CCATTTCTAG CCATCGGTTA TTGGATTGCG 
CCGTGTAGCA GGCTTGGAAG AATTCTTCAA AGCCCATTTA TGAAATTTGT GGCACATGCA 
GCATCCTTCA TCATTTTCCT AGGCCTGCTG GTGTTTAATG CTTCTGACAG ATTTGAAGGT 
ATAACAACGC TACCGAATGT CACTGTTACC GATTACCCTA AGCAGATCTT TAGGGTGAAG 
ACTACCCAGT TCACCTGGAC AGAAATGCTG ATCATGGTAT GGGTACTTGG TATGATGTGG 
TCAGAATGCA AAGAGCTGTG GCTGGAAGGA CCCAGGGAAT ATATTTTGCA GTTATGGAAT 
GTTTTGGATT TTGGGATGTT GTCCATTTTC ATTGCAGCTT TCACAGCGAG GCTTCTAGCA 
TTCCTGCAGG CCACAAAAGC ACAACAGTAT GTGGACAACT ACATTGAAGA GAGTGACCTC 
TCTGAGGTCA CGCTTCCTCC AGAAATAGAA TATTTTACCT ATGCTAGAGA TAAATGGCTT 
CCATCTGATC CTCAGATAAT ATCTGAAGGC CTTTATGCAA TTGCTGTTGT TCTTAGCTTT 
TCTCGGATTG CATATATCCT GCCTGCGAAT GAGAGTTTTG GACCCTTGCA GATTTCTCTT 
GGAAGGACTG TTAAAGACAT CTTCAAGTTT ATGGTCCTTT TTATTATGGT ATTTCTTGCA 
TTCATGATTG GAATGTTCAT ACTGTATTCC TACTACCTTG GAGCTAAGCT AAACCCAGCC 
TTTACAACAG TTGAAGAAAG CTTCAAAACC TTATTTTGGT CAATATTTGG CTTGTCTGAA 
GTAACCTCTG TTGTCCTGAA ATACGATCAT AAGTTCATTG AGAACATTGG ATATGTTCTC 
TATGGCATAT ACAATGTCAC GATGGTGGTA GTTCTGCTCA ACATGCTGAT TGCTATGATC 
AACAGCTCAT ATCAAGAAAT CGAGGATGAC AGTGATGTGG AGTGGAAGTT TGCTCGTTCT 
AAACTTTGGT TATCGTATTT TGATGATGGA AAAACATTAC CTCCACCATT TAGTCTTGTA 
CCAAGTCCCA AATCGTTTTT CTATTTCATC ATGAGGATCA TTAACTTTTC TAAGTGCAGG 
AGGAGACGGC TGCAGAAGGA CATTGAAATG GGAATGGGCA ATTCCAAATC GAGGTTAAAC 
CTTTTCACTC AGTCGAACTC CCGAGTTTTT GAATCACACA GTTTTAACAG CATTCTCAAT 
CAGCCAACAC GCTATCAGCA AATAATGAAA AGACTGATAA AACGTTACGT TCTGAAAGCA 
CAAGTGGACA AAGAAAATGA TGAAGTAAAT GAAGGTGAAT TAAAGGAAAT CAAACAAGAT 
ATCTCCAGTC TTCGCTATGA ACTTTTGGAG GACAAGTCCC AGGCGACAGA AGAGTTAGCC 
ATTTTAATAC ATAAACTCAG CGAGAAACTC AATCCCAATA TGTTAAGATG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131589.1
CDS	345..2861
Translation	MTMMREKGRRQAIRGPAFMFNNRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYIRIVEAILNHPGFSVNKRLTLSPCEQELQDDDFYSYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCNDCTEKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGDLNSEPVETQRHRASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGLPFLAIGYWIAPCSRLGRILQSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNVTVTDYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARLLAFLQATKAQQYVDNYIEESDLSEVTLPPEIEYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVLFIMVFLAFMIGMFILYSYYLGAKLNPAFTTVEESFKTLFWSIFGLSEVTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLVPSPKSFFYFIMRIINFSKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPNMLRCE

Target ORF information:

RefSeq Version	XM_015276103.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276103.1

ORF Insert Sequence:

ATGACTATGA TGAGGGAAAA GGGAAGGCGC CAGGCCATTA GAGGCCCAGC ATTCATGTTC 
AACAACAGAG GCACTAGTCT GACTGCTGAG GAAGAGCGCT TCCTAGATGC AGCAGAGTAT 
GGGAACATAC CTGTGGTGCG CAAAATGCTG GAGGAGTCAA AAACACTGAA TGTCAACTGT 
GTTGACTACA TGGGCCAAAA CGCCTTGCAG CTTGCTGTAG GGAACGAACA TTTGGAAGTG 
ACAGAACTTC TGTTAAAAAA AGAGAACTTG GCACGGATTG GAGATGCCCT GCTGCTCGCA 
ATCAGCAAGG GCTACATTAG GATAGTGGAA GCAATTTTAA ATCATCCTGG GTTTTCAGTA 
AATAAGCGAC TGACTCTGAG CCCTTGTGAG CAGGAGCTCC AGGATGACGA TTTTTATTCC 
TATGACGAAG ACGGCACCCG CTTTTCTCCA GACATCACTC CCATCATTTT AGCTGCTCAC 
TGCCAAAAAT ACGAAGTCGT GCATATGCTG TTGATGAAAG GAGCAAGGAT AGAAAGGCCT 
CATGATTATT TCTGCAAATG TAATGACTGT ACAGAGAAGC AAAAGCATGA TTCTTTCAGT 
CACTCTCGGT CAAGAATAAA TGCCTACAAA GGACTGGCCA GTCCAGCTTA CTTGTCTCTC 
TCCAGTGAAG ACCCTGTGCT CACTGCTCTG GAACTCAGCA ATGAGCTTGC CAAATTGGCT 
AACATTGAAA AAGAATTCAA GAATGACTAT CGCAAGCTGT CTATGCAATG CAAAGATTTT 
GTGGTTGGAG TTCTTGATCT CTGCCGAGAC TCGGAAGAAG TGGAGGCCAT CCTTAATGGG 
GATTTGAATT CAGAGCCGGT TGAAACGCAG AGACACAGAG CATCACTGAG CCGTGTGAAG 
CTGGCCATTA AGTATGAAGT CAAAAAGTTT GTGGCTCATC CAAACTGTCA GCAACAGCTA 
CTGACTATCT GGTATGAAAA TCTCTCAGGA CTGCGGGAGC AAACCATAGC CATCAAGTGT 
CTGGTTGTGC TGGTTGTGGC GTTGGGCCTT CCATTTCTAG CCATCGGTTA TTGGATTGCG 
CCGTGTAGCA GGCTTGGAAG AATTCTTCAA AGCCCATTTA TGAAATTTGT GGCACATGCA 
GCATCCTTCA TCATTTTCCT AGGCCTGCTG GTGTTTAATG CTTCTGACAG ATTTGAAGGT 
ATAACAACGC TACCGAATGT CACTGTTACC GATTACCCTA AGCAGATCTT TAGGGTGAAG 
ACTACCCAGT TCACCTGGAC AGAAATGCTG ATCATGGTAT GGGTACTTGG TATGATGTGG 
TCAGAATGCA AAGAGCTGTG GCTGGAAGGA CCCAGGGAAT ATATTTTGCA GTTATGGAAT 
GTTTTGGATT TTGGGATGTT GTCCATTTTC ATTGCAGCTT TCACAGCGAG GCTTCTAGCA 
TTCCTGCAGG CCACAAAAGC ACAACAGTAT GTGGACAACT ACATTGAAGA GAGTGACCTC 
TCTGAGGTCA CGCTTCCTCC AGAAATAGAA TATTTTACCT ATGCTAGAGA TAAATGGCTT 
CCATCTGATC CTCAGATAAT ATCTGAAGGC CTTTATGCAA TTGCTGTTGT TCTTAGCTTT 
TCTCGGATTG CATATATCCT GCCTGCGAAT GAGAGTTTTG GACCCTTGCA GATTTCTCTT 
GGAAGGACTG TTAAAGACAT CTTCAAGTTT ATGGTCCTTT TTATTATGGT ATTTCTTGCA 
TTCATGATTG GAATGTTCAT ACTGTATTCC TACTACCTTG GAGCTAAGCT AAACCCAGCC 
TTTACAACAG TTGAAGAAAG CTTCAAAACC TTATTTTGGT CAATATTTGG CTTGTCTGAA 
GTAACCTCTG TTGTCCTGAA ATACGATCAT AAGTTCATTG AGAACATTGG ATATGTTCTC 
TATGGCATAT ACAATGTCAC GATGGTGGTA GTTCTGCTCA ACATGCTGAT TGCTATGATC 
AACAGCTCAT ATCAAGAAAT CGAGGATGAC AGTGATGTGG AGTGGAAGTT TGCTCGTTCT 
AAACTTTGGT TATCGTATTT TGATGATGGA AAAACATTAC CTCCACCATT TAGTCTTGTA 
CCAAGTCCCA AATCGTTTTT CTATTTCATC ATGAGGATCA TTAACTTTTC TAAGTGCAGG 
AGGAGACGGC TGCAGAAGGA CATTGAAATG GGAATGGGCA ATTCCAAATC GAGGTTAAAC 
CTTTTCACTC AGTCGAACTC CCGAGTTTTT GAATCACACA GTTTTAACAG CATTCTCAAT 
CAGCCAACAC GCTATCAGCA AATAATGAAA AGACTGATAA AACGTTACGT TCTGAAAGCA 
CAAGTGGACA AAGAAAATGA TGAAGTAAAT GAAGGTGAAT TAAAGGAAAT CAAACAAGAT 
ATCTCCAGTC TTCGCTATGA ACTTTTGGAG GACAAGTCCC AGGCGACAGA AGAGTTAGCC 
ATTTTAATAC ATAAACTCAG CGAGAAACTC AATCCCAATA TGTTAAGATG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of transient receptor potential canonical 3 (TRPC3)-mediated Ca2+ entry by A1 adenosine receptor in cardiomyocytes disturbs atrioventricular conduction.
The Journal of biological chemistry287(32)26688-701(2012 Aug)
Sabourin J,Antigny F,Robin E,Frieden M,Raddatz E

Ca2+ influx and protein scaffolding via TRPC3 sustain PKCbeta and ERK activation in B cells.
Journal of cell science123(Pt 6)927-38(2010 Mar)
Numaga T,Nishida M,Kiyonaka S,Kato K,Katano M,Mori E,Kurosaki T,Inoue R,Hikida M,Putney JW Jr,Mori Y

TRPC3-like protein and vitamin D receptor mediate 1alpha,25(OH)2D3-induced SOC influx in muscle cells.
The international journal of biochemistry & cell biology36(10)1910-8(2004 Oct)
Santill?n G,Katz S,Vazquez G,Boland RL

Evidence that TRPC3 is a molecular component of the 1alpha,25(OH)2D3-activated capacitative calcium entry (CCE) in muscle and osteoblast cells.
The Journal of steroid biochemistry and molecular biology89-90(1-5)291-5(2004 May)
Santill?n G,Baldi C,Katz S,Vazquez G,Boland R

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

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TRPC3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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