ROBO2 cDNA ORF clone, Gallus gallus(chicken)

The following ROBO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142919 XM_025144745.1
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa142920 XM_025144753.1
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa142921 XM_025144759.1
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa30220 XM_015298609.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30221 XM_015298613.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30222 XM_015298618.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30223 XM_015298628.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa142918 XM_025144743.1
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa30208 XM_015298528.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30209 XM_015298532.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30210 XM_015298535.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30211 XM_015298540.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30212 XM_015298548.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30213 XM_015298556.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30214 XM_015298560.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30215 XM_015298564.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30216 XM_015298573.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30217 XM_015298584.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30218 XM_015298594.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30219 XM_015298603.2
Latest version!
Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OGa30217 XM_015298584.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30221 XM_015298613.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30220 XM_015298609.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30216 XM_015298573.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30218 XM_015298594.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30223 XM_015298628.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30222 XM_015298618.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa30215 XM_015298564.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30209 XM_015298532.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30212 XM_015298548.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30213 XM_015298556.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30208 XM_015298528.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30210 XM_015298535.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30211 XM_015298540.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30214 XM_015298560.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30219 XM_015298603.1 Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142919
    Clone ID Related Accession (Same CDS sequence) XM_025144745.1
    Accession Version XM_025144745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAA GAGGCACTGG TACCATACAG TAAACCCAAC
    TTCCCATCCC CTGGTGGCCA CAGCTCTTCA GGAACAGCTT CTTCTAAAGG ATCGACTGGA
    CCTAGAAAAG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000513.1
    CDS381..4835
    Translation

    Target ORF information:

    RefSeq Version XM_025144745.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAA GAGGCACTGG TACCATACAG TAAACCCAAC
    TTCCCATCCC CTGGTGGCCA CAGCTCTTCA GGAACAGCTT CTTCTAAAGG ATCGACTGGA
    CCTAGAAAAG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa142920
    Clone ID Related Accession (Same CDS sequence) XM_025144753.1
    Accession Version XM_025144753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGAAGAG GCACTGGTAC CATACAGTAA ACCCAACTTC
    CCATCCCCTG GTGGCCACAG CTCTTCAGGA ACAGCTTCTT CTAAAGGATC GACTGGACCT
    AGAAAAGGTG AAGTCTTTCG AGGAGGTCAC CAACGCAATG CCAGTGACCT TCTTGATGTA
    GGCTATTTGG GATCAAACAG TCAAGGACAG TTTACTGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000521.1
    CDS377..4744
    Translation

    Target ORF information:

    RefSeq Version XM_025144753.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGAAGAG GCACTGGTAC CATACAGTAA ACCCAACTTC
    CCATCCCCTG GTGGCCACAG CTCTTCAGGA ACAGCTTCTT CTAAAGGATC GACTGGACCT
    AGAAAAGGTG AAGTCTTTCG AGGAGGTCAC CAACGCAATG CCAGTGACCT TCTTGATGTA
    GGCTATTTGG GATCAAACAG TCAAGGACAG TTTACTGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa142921
    Clone ID Related Accession (Same CDS sequence) XM_025144759.1
    Accession Version XM_025144759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GGTGGCAAAG GAGGGAAAAA GAAGAAAAGC AAAAATACTG CCAAGCCTCA GAAAAGTAAT
    GGTTCAAACT GGGCCAGTGT TCCCCTCCCA CCCCCTCCTG TGCAGCCGCT TCCAGGCACA
    GAGCTGGAAC ACTACGGGGT GGATCAACAA GAAGCAGGAT ATGACAGTGA TGGTTGGTGT
    CCACCCTTGC CTGTTCAGAC CTACCTCCAT CAGGGCATGG AGGATGAGCT TGAAGAAGAT
    GATGATCGTG TTCCCACACC TCCTGTGCGA GGAGTAGCTT CATCACCTGC GATCTCCTTT
    GGGCAACAAT CAACTGCAAC CCTCACGCCT TCTCCGCGAG AGGAGATGCA GCCAATGCTT
    CAAGCCCACC TCGATGAATT AACCAGGGCC TACCAGTTTG ATATAGCAAA GCAGACATGG
    CACATCCAAA GCAATACACA ACCTCCTCAG GCTCCAGTTC CACCCCTAGG ATATGTATCT
    GGAAACCTTA TTTCAGACTT GGAAACAGAC GTTCCAGATG ATGATGAGGA TGATATTGAA
    GAAGAAACTC TAGAAATCAA TAGGCCACTG AGAGGTCTAG AGCATACTCC AGGCTCCAGT
    ATGGACAATC TAGACAGCTC TGTGACAGGC AAAGCATATC CTTCCTCTCA GAGACCTCGG
    CCAAGCAGTC CTTTTTCTAC TGACAGCAAC ACCAGTGCAG CTGTCACTCA GAGTCAGAGG
    CCAAGGCCCA CTAAAAAACA CAAGGGAGGC CGGTTGGACC AACCCCCCTT GCCTCATCGT
    AGGGAAGGAA TAACAGACGA AGAGGCACTG GTACCATACA GTAAACCCAA CTTCCCATCC
    CCTGGTGGCC ACAGCTCTTC AGGAACAGCT TCTTCTAAAG GATCGACTGG ACCTAGAAAA
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000527.1
    CDS373..4518
    Translation

    Target ORF information:

    RefSeq Version XM_025144759.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GGTGGCAAAG GAGGGAAAAA GAAGAAAAGC AAAAATACTG CCAAGCCTCA GAAAAGTAAT
    GGTTCAAACT GGGCCAGTGT TCCCCTCCCA CCCCCTCCTG TGCAGCCGCT TCCAGGCACA
    GAGCTGGAAC ACTACGGGGT GGATCAACAA GAAGCAGGAT ATGACAGTGA TGGTTGGTGT
    CCACCCTTGC CTGTTCAGAC CTACCTCCAT CAGGGCATGG AGGATGAGCT TGAAGAAGAT
    GATGATCGTG TTCCCACACC TCCTGTGCGA GGAGTAGCTT CATCACCTGC GATCTCCTTT
    GGGCAACAAT CAACTGCAAC CCTCACGCCT TCTCCGCGAG AGGAGATGCA GCCAATGCTT
    CAAGCCCACC TCGATGAATT AACCAGGGCC TACCAGTTTG ATATAGCAAA GCAGACATGG
    CACATCCAAA GCAATACACA ACCTCCTCAG GCTCCAGTTC CACCCCTAGG ATATGTATCT
    GGAAACCTTA TTTCAGACTT GGAAACAGAC GTTCCAGATG ATGATGAGGA TGATATTGAA
    GAAGAAACTC TAGAAATCAA TAGGCCACTG AGAGGTCTAG AGCATACTCC AGGCTCCAGT
    ATGGACAATC TAGACAGCTC TGTGACAGGC AAAGCATATC CTTCCTCTCA GAGACCTCGG
    CCAAGCAGTC CTTTTTCTAC TGACAGCAAC ACCAGTGCAG CTGTCACTCA GAGTCAGAGG
    CCAAGGCCCA CTAAAAAACA CAAGGGAGGC CGGTTGGACC AACCCCCCTT GCCTCATCGT
    AGGGAAGGAA TAACAGACGA AGAGGCACTG GTACCATACA GTAAACCCAA CTTCCCATCC
    CCTGGTGGCC ACAGCTCTTC AGGAACAGCT TCTTCTAAAG GATCGACTGG ACCTAGAAAA
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30220
    Clone ID Related Accession (Same CDS sequence) XM_015298609.1 , XM_015298609.2
    Accession Version XM_015298609.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGATG GGGTTCTGCG TCTGATGAGG ACCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGAC TCGATCTTTA CCAGCGGCAG TTTTGCACAA
    GCACTGGTTG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTGACAAGA
    ACAGGCAAAG CATATCCTTC CTCTCAGAGA CCTCGGCCAA GCAGTCCTTT TTCTACTGAC
    AGCAACACCA GTGCAGCTGT CACTCAGAGT CAGAGGCCAA GGCCCACTAA AAAACACAAG
    GGAGGCCGGT TGGACCAACC CCCCTTGCCT CATCGTAGGG AAGGAATAAC AGACGAAGAG
    GCACTGGTAC CATACAGTAA ACCCAACTTC CCATCCCCTG GTGGCCACAG CTCTTCAGGA
    ACAGCTTCTT CTAAAGGATC GACTGGACCT AGAAAAGGTG AAGTCTTTCG AGGAGGTCAC
    CAACGCAATG CCAGTGACCT TCTTGATGTA GGCTATTTGG GATCAAACAG TCAAGGACAG
    TTTACTGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154095.1
    CDS408..4805
    Translation

    Target ORF information:

    RefSeq Version XM_015298609.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGATG GGGTTCTGCG TCTGATGAGG ACCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGAC TCGATCTTTA CCAGCGGCAG TTTTGCACAA
    GCACTGGTTG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTGACAAGA
    ACAGGCAAAG CATATCCTTC CTCTCAGAGA CCTCGGCCAA GCAGTCCTTT TTCTACTGAC
    AGCAACACCA GTGCAGCTGT CACTCAGAGT CAGAGGCCAA GGCCCACTAA AAAACACAAG
    GGAGGCCGGT TGGACCAACC CCCCTTGCCT CATCGTAGGG AAGGAATAAC AGACGAAGAG
    GCACTGGTAC CATACAGTAA ACCCAACTTC CCATCCCCTG GTGGCCACAG CTCTTCAGGA
    ACAGCTTCTT CTAAAGGATC GACTGGACCT AGAAAAGGTG AAGTCTTTCG AGGAGGTCAC
    CAACGCAATG CCAGTGACCT TCTTGATGTA GGCTATTTGG GATCAAACAG TCAAGGACAG
    TTTACTGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30220
    Clone ID Related Accession (Same CDS sequence) XM_015298609.1 , XM_015298609.2
    Accession Version XM_015298609.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGATG GGGTTCTGCG TCTGATGAGG ACCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGAC TCGATCTTTA CCAGCGGCAG TTTTGCACAA
    GCACTGGTTG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTGACAAGA
    ACAGGCAAAG CATATCCTTC CTCTCAGAGA CCTCGGCCAA GCAGTCCTTT TTCTACTGAC
    AGCAACACCA GTGCAGCTGT CACTCAGAGT CAGAGGCCAA GGCCCACTAA AAAACACAAG
    GGAGGCCGGT TGGACCAACC CCCCTTGCCT CATCGTAGGG AAGGAATAAC AGACGAAGAG
    GCACTGGTAC CATACAGTAA ACCCAACTTC CCATCCCCTG GTGGCCACAG CTCTTCAGGA
    ACAGCTTCTT CTAAAGGATC GACTGGACCT AGAAAAGGTG AAGTCTTTCG AGGAGGTCAC
    CAACGCAATG CCAGTGACCT TCTTGATGTA GGCTATTTGG GATCAAACAG TCAAGGACAG
    TTTACTGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154095.1
    CDS375..4772
    Translation

    Target ORF information:

    RefSeq Version XM_015298609.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298609.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGATG GGGTTCTGCG TCTGATGAGG ACCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGAC TCGATCTTTA CCAGCGGCAG TTTTGCACAA
    GCACTGGTTG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTGACAAGA
    ACAGGCAAAG CATATCCTTC CTCTCAGAGA CCTCGGCCAA GCAGTCCTTT TTCTACTGAC
    AGCAACACCA GTGCAGCTGT CACTCAGAGT CAGAGGCCAA GGCCCACTAA AAAACACAAG
    GGAGGCCGGT TGGACCAACC CCCCTTGCCT CATCGTAGGG AAGGAATAAC AGACGAAGAG
    GCACTGGTAC CATACAGTAA ACCCAACTTC CCATCCCCTG GTGGCCACAG CTCTTCAGGA
    ACAGCTTCTT CTAAAGGATC GACTGGACCT AGAAAAGGTG AAGTCTTTCG AGGAGGTCAC
    CAACGCAATG CCAGTGACCT TCTTGATGTA GGCTATTTGG GATCAAACAG TCAAGGACAG
    TTTACTGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30221
    Clone ID Related Accession (Same CDS sequence) XM_015298613.2 , XM_015298613.1
    Accession Version XM_015298613.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGAAGAG GCACTGGTAC CATACAGTAA ACCCAACTTC
    CCATCCCCTG GTGGCCACAG CTCTTCAGGA ACAGCTTCTT CTAAAGGATC GACTGGACCT
    AGAAAAGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154099.1
    CDS375..4646
    Translation

    Target ORF information:

    RefSeq Version XM_015298613.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298613.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGAAGAG GCACTGGTAC CATACAGTAA ACCCAACTTC
    CCATCCCCTG GTGGCCACAG CTCTTCAGGA ACAGCTTCTT CTAAAGGATC GACTGGACCT
    AGAAAAGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30221
    Clone ID Related Accession (Same CDS sequence) XM_015298613.2 , XM_015298613.1
    Accession Version XM_015298613.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGAAGAG GCACTGGTAC CATACAGTAA ACCCAACTTC
    CCATCCCCTG GTGGCCACAG CTCTTCAGGA ACAGCTTCTT CTAAAGGATC GACTGGACCT
    AGAAAAGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154099.1
    CDS405..4676
    Translation

    Target ORF information:

    RefSeq Version XM_015298613.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGAAGAG GCACTGGTAC CATACAGTAA ACCCAACTTC
    CCATCCCCTG GTGGCCACAG CTCTTCAGGA ACAGCTTCTT CTAAAGGATC GACTGGACCT
    AGAAAAGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30222
    Clone ID Related Accession (Same CDS sequence) XM_015298618.1 , XM_015298618.2
    Accession Version XM_015298618.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCAT ATCCTTCCTC TCAGAGACCT CGGCCAAGCA GTCCTTTTTC TACTGACAGC
    AACACCAGTG CAGCTGTCAC TCAGAGTCAG AGGCCAAGGC CCACTAAAAA ACACAAGGGA
    GGCCGGTTGG ACCAACCCCC CTTGCCTCAT CGTAGGGAAG GAATAACAGA CGAAGAGGCA
    CTGGTACCAT ACAGTAAACC CAACTTCCCA TCCCCTGGTG GCCACAGCTC TTCAGGAACA
    GCTTCTTCTA AAGGATCGAC TGGACCTAGA AAAGGTGAAG TCTTTCGAGG AGGTCACCAA
    CGCAATGCCA GTGACCTTCT TGATGTAGGC TATTTGGGAT CAAACAGTCA AGGACAGTTT
    ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154104.1
    CDS400..4614
    Translation

    Target ORF information:

    RefSeq Version XM_015298618.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCAT ATCCTTCCTC TCAGAGACCT CGGCCAAGCA GTCCTTTTTC TACTGACAGC
    AACACCAGTG CAGCTGTCAC TCAGAGTCAG AGGCCAAGGC CCACTAAAAA ACACAAGGGA
    GGCCGGTTGG ACCAACCCCC CTTGCCTCAT CGTAGGGAAG GAATAACAGA CGAAGAGGCA
    CTGGTACCAT ACAGTAAACC CAACTTCCCA TCCCCTGGTG GCCACAGCTC TTCAGGAACA
    GCTTCTTCTA AAGGATCGAC TGGACCTAGA AAAGGTGAAG TCTTTCGAGG AGGTCACCAA
    CGCAATGCCA GTGACCTTCT TGATGTAGGC TATTTGGGAT CAAACAGTCA AGGACAGTTT
    ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30222
    Clone ID Related Accession (Same CDS sequence) XM_015298618.1 , XM_015298618.2
    Accession Version XM_015298618.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCAT ATCCTTCCTC TCAGAGACCT CGGCCAAGCA GTCCTTTTTC TACTGACAGC
    AACACCAGTG CAGCTGTCAC TCAGAGTCAG AGGCCAAGGC CCACTAAAAA ACACAAGGGA
    GGCCGGTTGG ACCAACCCCC CTTGCCTCAT CGTAGGGAAG GAATAACAGA CGAAGAGGCA
    CTGGTACCAT ACAGTAAACC CAACTTCCCA TCCCCTGGTG GCCACAGCTC TTCAGGAACA
    GCTTCTTCTA AAGGATCGAC TGGACCTAGA AAAGGTGAAG TCTTTCGAGG AGGTCACCAA
    CGCAATGCCA GTGACCTTCT TGATGTAGGC TATTTGGGAT CAAACAGTCA AGGACAGTTT
    ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154104.1
    CDS372..4586
    Translation

    Target ORF information:

    RefSeq Version XM_015298618.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGGTGGCA AAGGAGGGAA AAAGAAGAAA
    AGCAAAAATA CTGCCAAGCC TCAGAAAAGT AATGGTTCAA ACTGGGCCAG TGTTCCCCTC
    CCACCCCCTC CTGTGCAGCC GCTTCCAGGC ACAGAGCTGG AACACTACGG GGTGGATCAA
    CAAGAAGCAG GATATGACAG TGATGGTTGG TGTCCACCCT TGCCTGTTCA GACCTACCTC
    CATCAGGGCA TGGAGGATGA GCTTGAAGAA GATGATGATC GTGTTCCCAC ACCTCCTGTG
    CGAGGAGTAG CTTCATCACC TGCGATCTCC TTTGGGCAAC AATCAACTGC AACCCTCACG
    CCTTCTCCGC GAGAGGAGAT GCAGCCAATG CTTCAAGCCC ACCTCGATGA ATTAACCAGG
    GCCTACCAGT TTGATATAGC AAAGCAGACA TGGCACATCC AAAGCAATAC ACAACCTCCT
    CAGGCTCCAG TTCCACCCCT AGGATATGTA TCTGGAAACC TTATTTCAGA CTTGGAAACA
    GACGTTCCAG ATGATGATGA GGATGATATT GAAGAAGAAA CTCTAGAAAT CAATAGGCCA
    CTGAGAGGTC TAGAGCATAC TCCAGGCTCC AGTATGGACA ATCTAGACAG CTCTGTGACA
    GGCAAAGCAT ATCCTTCCTC TCAGAGACCT CGGCCAAGCA GTCCTTTTTC TACTGACAGC
    AACACCAGTG CAGCTGTCAC TCAGAGTCAG AGGCCAAGGC CCACTAAAAA ACACAAGGGA
    GGCCGGTTGG ACCAACCCCC CTTGCCTCAT CGTAGGGAAG GAATAACAGA CGAAGAGGCA
    CTGGTACCAT ACAGTAAACC CAACTTCCCA TCCCCTGGTG GCCACAGCTC TTCAGGAACA
    GCTTCTTCTA AAGGATCGAC TGGACCTAGA AAAGGTGAAG TCTTTCGAGG AGGTCACCAA
    CGCAATGCCA GTGACCTTCT TGATGTAGGC TATTTGGGAT CAAACAGTCA AGGACAGTTT
    ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30223
    Clone ID Related Accession (Same CDS sequence) XM_015298628.2 , XM_015298628.1
    Accession Version XM_015298628.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT CACATTCCAA
    AGAGGAGATG GAGGACTAAT GAGCAATGGA AGTCGACCAG GTTTGTTGAA TGCAAGTGAC
    CCCAGTTATC CTTGGCTTGC AGACTCTTGG CCTGCCACAA GTCTGCCAGT TAACAACAGC
    TCTAGTGGAC CCAATGACCT TGCCAATTTC AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA
    GGACAAGGAG ATAAAACTGC TACTATGCTT TCTGATGGAG CCATTTACAG CAGCATTGAC
    TTCACGACAA AAACTAGTTA CAACAGTTCC AGCCAAATAA CGCAAGCTAC ACCATATGCC
    ACCACCCAGA TACTGCATTC CAACAGCATC CATGAGTTGG CAGTCGACTT ACCTGATCCT
    CAGTGGAAAA GCTCAATTCA GCAAAAAAAT GACCTGATGG GATTTGGCTA CTCTCTCCCA
    GACCAAGGCA AAGGCAACAA TGGTGGCAAA GGAGGGAAAA AGAAGAAAAG CAAAAATACT
    GCCAAGCCTC AGAAAAGTAA TGGTTCAAAC TGGGCCAGTG TTCCCCTCCC ACCCCCTCCT
    GTGCAGCCGC TTCCAGGCAC AGAGCTGGAA CACTACGGGG TGGATCAACA AGAAGCAGGA
    TATGACAGTG ATGGTTGGTG TCCACCCTTG CCTGTTCAGA CCTACCTCCA TCAGGGCATG
    GAGGATGAGC TTGAAGAAGA TGATGATCGT GTTCCCACAC CTCCTGTGCG AGGAGTAGCT
    TCATCACCTG CGATCTCCTT TGGGCAACAA TCAACTGCAA CCCTCACGCC TTCTCCGCGA
    GAGGAGATGC AGCCAATGCT TCAAGCCCAC CTCGATGAAT TAACCAGGGC CTACCAGTTT
    GATATAGCAA AGCAGACATG GCACATCCAA AGCAATACAC AACCTCCTCA GGCTCCAGTT
    CCACCCCTAG GATATGTATC TGGAAACCTT ATTTCAGACT TGGAAACAGA CGTTCCAGAT
    GATGATGAGG ATGATATTGA AGAAGAAACT CTAGAAATCA ATAGGCCACT GAGAGGTCTA
    GAGCATACTC CAGGCTCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCATAT
    CCTTCCTCTC AGAGACCTCG GCCAAGCAGT CCTTTTTCTA CTGACAGCAA CACCAGTGCA
    GCTGTCACTC AGAGTCAGAG GCCAAGGCCC ACTAAAAAAC ACAAGGGAGG CCGGTTGGAC
    CAACCCCCCT TGCCTCATCG TAGGGAAGGA ATAACAGACG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154114.1
    CDS373..4575
    Translation

    Target ORF information:

    RefSeq Version XM_015298628.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298628.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT CACATTCCAA
    AGAGGAGATG GAGGACTAAT GAGCAATGGA AGTCGACCAG GTTTGTTGAA TGCAAGTGAC
    CCCAGTTATC CTTGGCTTGC AGACTCTTGG CCTGCCACAA GTCTGCCAGT TAACAACAGC
    TCTAGTGGAC CCAATGACCT TGCCAATTTC AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA
    GGACAAGGAG ATAAAACTGC TACTATGCTT TCTGATGGAG CCATTTACAG CAGCATTGAC
    TTCACGACAA AAACTAGTTA CAACAGTTCC AGCCAAATAA CGCAAGCTAC ACCATATGCC
    ACCACCCAGA TACTGCATTC CAACAGCATC CATGAGTTGG CAGTCGACTT ACCTGATCCT
    CAGTGGAAAA GCTCAATTCA GCAAAAAAAT GACCTGATGG GATTTGGCTA CTCTCTCCCA
    GACCAAGGCA AAGGCAACAA TGGTGGCAAA GGAGGGAAAA AGAAGAAAAG CAAAAATACT
    GCCAAGCCTC AGAAAAGTAA TGGTTCAAAC TGGGCCAGTG TTCCCCTCCC ACCCCCTCCT
    GTGCAGCCGC TTCCAGGCAC AGAGCTGGAA CACTACGGGG TGGATCAACA AGAAGCAGGA
    TATGACAGTG ATGGTTGGTG TCCACCCTTG CCTGTTCAGA CCTACCTCCA TCAGGGCATG
    GAGGATGAGC TTGAAGAAGA TGATGATCGT GTTCCCACAC CTCCTGTGCG AGGAGTAGCT
    TCATCACCTG CGATCTCCTT TGGGCAACAA TCAACTGCAA CCCTCACGCC TTCTCCGCGA
    GAGGAGATGC AGCCAATGCT TCAAGCCCAC CTCGATGAAT TAACCAGGGC CTACCAGTTT
    GATATAGCAA AGCAGACATG GCACATCCAA AGCAATACAC AACCTCCTCA GGCTCCAGTT
    CCACCCCTAG GATATGTATC TGGAAACCTT ATTTCAGACT TGGAAACAGA CGTTCCAGAT
    GATGATGAGG ATGATATTGA AGAAGAAACT CTAGAAATCA ATAGGCCACT GAGAGGTCTA
    GAGCATACTC CAGGCTCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCATAT
    CCTTCCTCTC AGAGACCTCG GCCAAGCAGT CCTTTTTCTA CTGACAGCAA CACCAGTGCA
    GCTGTCACTC AGAGTCAGAG GCCAAGGCCC ACTAAAAAAC ACAAGGGAGG CCGGTTGGAC
    CAACCCCCCT TGCCTCATCG TAGGGAAGGA ATAACAGACG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30223
    Clone ID Related Accession (Same CDS sequence) XM_015298628.2 , XM_015298628.1
    Accession Version XM_015298628.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT CACATTCCAA
    AGAGGAGATG GAGGACTAAT GAGCAATGGA AGTCGACCAG GTTTGTTGAA TGCAAGTGAC
    CCCAGTTATC CTTGGCTTGC AGACTCTTGG CCTGCCACAA GTCTGCCAGT TAACAACAGC
    TCTAGTGGAC CCAATGACCT TGCCAATTTC AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA
    GGACAAGGAG ATAAAACTGC TACTATGCTT TCTGATGGAG CCATTTACAG CAGCATTGAC
    TTCACGACAA AAACTAGTTA CAACAGTTCC AGCCAAATAA CGCAAGCTAC ACCATATGCC
    ACCACCCAGA TACTGCATTC CAACAGCATC CATGAGTTGG CAGTCGACTT ACCTGATCCT
    CAGTGGAAAA GCTCAATTCA GCAAAAAAAT GACCTGATGG GATTTGGCTA CTCTCTCCCA
    GACCAAGGCA AAGGCAACAA TGGTGGCAAA GGAGGGAAAA AGAAGAAAAG CAAAAATACT
    GCCAAGCCTC AGAAAAGTAA TGGTTCAAAC TGGGCCAGTG TTCCCCTCCC ACCCCCTCCT
    GTGCAGCCGC TTCCAGGCAC AGAGCTGGAA CACTACGGGG TGGATCAACA AGAAGCAGGA
    TATGACAGTG ATGGTTGGTG TCCACCCTTG CCTGTTCAGA CCTACCTCCA TCAGGGCATG
    GAGGATGAGC TTGAAGAAGA TGATGATCGT GTTCCCACAC CTCCTGTGCG AGGAGTAGCT
    TCATCACCTG CGATCTCCTT TGGGCAACAA TCAACTGCAA CCCTCACGCC TTCTCCGCGA
    GAGGAGATGC AGCCAATGCT TCAAGCCCAC CTCGATGAAT TAACCAGGGC CTACCAGTTT
    GATATAGCAA AGCAGACATG GCACATCCAA AGCAATACAC AACCTCCTCA GGCTCCAGTT
    CCACCCCTAG GATATGTATC TGGAAACCTT ATTTCAGACT TGGAAACAGA CGTTCCAGAT
    GATGATGAGG ATGATATTGA AGAAGAAACT CTAGAAATCA ATAGGCCACT GAGAGGTCTA
    GAGCATACTC CAGGCTCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCATAT
    CCTTCCTCTC AGAGACCTCG GCCAAGCAGT CCTTTTTCTA CTGACAGCAA CACCAGTGCA
    GCTGTCACTC AGAGTCAGAG GCCAAGGCCC ACTAAAAAAC ACAAGGGAGG CCGGTTGGAC
    CAACCCCCCT TGCCTCATCG TAGGGAAGGA ATAACAGACG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154114.1
    CDS402..4604
    Translation

    Target ORF information:

    RefSeq Version XM_015298628.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT CACATTCCAA
    AGAGGAGATG GAGGACTAAT GAGCAATGGA AGTCGACCAG GTTTGTTGAA TGCAAGTGAC
    CCCAGTTATC CTTGGCTTGC AGACTCTTGG CCTGCCACAA GTCTGCCAGT TAACAACAGC
    TCTAGTGGAC CCAATGACCT TGCCAATTTC AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA
    GGACAAGGAG ATAAAACTGC TACTATGCTT TCTGATGGAG CCATTTACAG CAGCATTGAC
    TTCACGACAA AAACTAGTTA CAACAGTTCC AGCCAAATAA CGCAAGCTAC ACCATATGCC
    ACCACCCAGA TACTGCATTC CAACAGCATC CATGAGTTGG CAGTCGACTT ACCTGATCCT
    CAGTGGAAAA GCTCAATTCA GCAAAAAAAT GACCTGATGG GATTTGGCTA CTCTCTCCCA
    GACCAAGGCA AAGGCAACAA TGGTGGCAAA GGAGGGAAAA AGAAGAAAAG CAAAAATACT
    GCCAAGCCTC AGAAAAGTAA TGGTTCAAAC TGGGCCAGTG TTCCCCTCCC ACCCCCTCCT
    GTGCAGCCGC TTCCAGGCAC AGAGCTGGAA CACTACGGGG TGGATCAACA AGAAGCAGGA
    TATGACAGTG ATGGTTGGTG TCCACCCTTG CCTGTTCAGA CCTACCTCCA TCAGGGCATG
    GAGGATGAGC TTGAAGAAGA TGATGATCGT GTTCCCACAC CTCCTGTGCG AGGAGTAGCT
    TCATCACCTG CGATCTCCTT TGGGCAACAA TCAACTGCAA CCCTCACGCC TTCTCCGCGA
    GAGGAGATGC AGCCAATGCT TCAAGCCCAC CTCGATGAAT TAACCAGGGC CTACCAGTTT
    GATATAGCAA AGCAGACATG GCACATCCAA AGCAATACAC AACCTCCTCA GGCTCCAGTT
    CCACCCCTAG GATATGTATC TGGAAACCTT ATTTCAGACT TGGAAACAGA CGTTCCAGAT
    GATGATGAGG ATGATATTGA AGAAGAAACT CTAGAAATCA ATAGGCCACT GAGAGGTCTA
    GAGCATACTC CAGGCTCCAG TATGGACAAT CTAGACAGCT CTGTGACAGG CAAAGCATAT
    CCTTCCTCTC AGAGACCTCG GCCAAGCAGT CCTTTTTCTA CTGACAGCAA CACCAGTGCA
    GCTGTCACTC AGAGTCAGAG GCCAAGGCCC ACTAAAAAAC ACAAGGGAGG CCGGTTGGAC
    CAACCCCCCT TGCCTCATCG TAGGGAAGGA ATAACAGACG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa142918
    Clone ID Related Accession (Same CDS sequence) XM_025144743.1
    Accession Version XM_025144743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGATCTT CCACCACCAC CTGACCCACC GCCTGGTCAG
    GGTTTAAGGC AGCAAATAGG CTCGGGCCAG CGCGGAGGCT CGGCAGAGAG GAAAGGAAGC
    TCTCTTGAGA GGCAGCATGC ACCCAGCGTA GATGACACAA AGAGCTCCTT GGACCGGCAA
    GCTAGGACGT CACTAGAGTG GCAGCGGCAA ACCCAAGAGT GGATAAGCTC TGCAGAACGC
    CAAGAGGATT TCAGGAAAGC CCAACACAAG CAAGCATTCG GATCAGAAGA GGCACTGGTA
    CCATACAGTA AACCCAACTT CCCATCCCCT GGTGGCCACA GCTCTTCAGG AACAGCTTCT
    TCTAAAGGAT CGACTGGACC TAGAAAAGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000511.1
    CDS374..4906
    Translation

    Target ORF information:

    RefSeq Version XM_025144743.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGCAAAG CATATCCTTC CTCTCAGAGA
    CCTCGGCCAA GCAGTCCTTT TTCTACTGAC AGCAACACCA GTGCAGCTGT CACTCAGAGT
    CAGAGGCCAA GGCCCACTAA AAAACACAAG GGAGGCCGGT TGGACCAACC CCCCTTGCCT
    CATCGTAGGG AAGGAATAAC AGACGATCTT CCACCACCAC CTGACCCACC GCCTGGTCAG
    GGTTTAAGGC AGCAAATAGG CTCGGGCCAG CGCGGAGGCT CGGCAGAGAG GAAAGGAAGC
    TCTCTTGAGA GGCAGCATGC ACCCAGCGTA GATGACACAA AGAGCTCCTT GGACCGGCAA
    GCTAGGACGT CACTAGAGTG GCAGCGGCAA ACCCAAGAGT GGATAAGCTC TGCAGAACGC
    CAAGAGGATT TCAGGAAAGC CCAACACAAG CAAGCATTCG GATCAGAAGA GGCACTGGTA
    CCATACAGTA AACCCAACTT CCCATCCCCT GGTGGCCACA GCTCTTCAGG AACAGCTTCT
    TCTAAAGGAT CGACTGGACC TAGAAAAGAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30208
    Clone ID Related Accession (Same CDS sequence) XM_015298528.2 , XM_015298528.1
    Accession Version XM_015298528.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GGTGAAGTCT TTCGAGGAGG TCACCAACGC
    AATGCCAGTG ACCTTCTTGA TGTAGGCTAT TTGGGATCAA ACAGTCAAGG ACAGTTTACT
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154014.1
    CDS379..5190
    Translation

    Target ORF information:

    RefSeq Version XM_015298528.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298528.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GGTGAAGTCT TTCGAGGAGG TCACCAACGC
    AATGCCAGTG ACCTTCTTGA TGTAGGCTAT TTGGGATCAA ACAGTCAAGG ACAGTTTACT
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30208
    Clone ID Related Accession (Same CDS sequence) XM_015298528.2 , XM_015298528.1
    Accession Version XM_015298528.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GGTGAAGTCT TTCGAGGAGG TCACCAACGC
    AATGCCAGTG ACCTTCTTGA TGTAGGCTAT TTGGGATCAA ACAGTCAAGG ACAGTTTACT
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154014.1
    CDS413..5224
    Translation

    Target ORF information:

    RefSeq Version XM_015298528.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GGTGAAGTCT TTCGAGGAGG TCACCAACGC
    AATGCCAGTG ACCTTCTTGA TGTAGGCTAT TTGGGATCAA ACAGTCAAGG ACAGTTTACT
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30209
    Clone ID Related Accession (Same CDS sequence) XM_015298532.1 , XM_015298532.2
    Accession Version XM_015298532.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT TCAGTCCTTC
    ACCTTCACCC CTGCAGTCAC ATTCCAAAGA GGAGATGGAG GACTAATGAG CAATGGAAGT
    CGACCAGGTT TGTTGAATGC AAGTGACCCC AGTTATCCTT GGCTTGCAGA CTCTTGGCCT
    GCCACAAGTC TGCCAGTTAA CAACAGCTCT AGTGGACCCA ATGACCTTGC CAATTTCAGT
    CGTGGAGATG TGTTGCCACC AGTGCCAGGA CAAGGAGATA AAACTGCTAC TATGCTTTCT
    GATGGAGCCA TTTACAGCAG CATTGACTTC ACGACAAAAA CTAGTTACAA CAGTTCCAGC
    CAAATAACGC AAGCTACACC ATATGCCACC ACCCAGATAC TGCATTCCAA CAGCATCCAT
    GAGTTGGCAG TCGACTTACC TGATCCTCAG TGGAAAAGCT CAATTCAGCA AAAAAATGAC
    CTGATGGGAT TTGGCTACTC TCTCCCAGAC CAAGGCAAAG GCAACAATGC CTTGCTTTAC
    ATCCCTGACT ACCGCGTGGC TGAGGGATTG GCTAATAGAT TGCCACACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC CCACAACAGC TCCGAAAGGA GTGGCAGCCT CTCAGGTGGC
    AAAGGAGGGA AAAAGAAGAA AAGCAAAAAT ACTGCCAAGC CTCAGAAAAG TAATGGTTCA
    AACTGGGCCA GTGTTCCCCT CCCACCCCCT CCTGTGCAGC CGCTTCCAGG CACAGAGCTG
    GAACACTACG GGGTGGATCA ACAAGAAGCA GGATATGACA GTGATGGTTG GTGTCCACCC
    TTGCCTGTTC AGACCTACCT CCATCAGGGC ATGGAGGATG AGCTTGAAGA AGATGATGAT
    CGTGTTCCCA CACCTCCTGT GCGAGGAGTA GCTTCATCAC CTGCGATCTC CTTTGGGCAA
    CAATCAACTG CAACCCTCAC GCCTTCTCCG CGAGAGGAGA TGCAGCCAAT GCTTCAAGCC
    CACCTCGATG AATTAACCAG GGCCTACCAG TTTGATATAG CAAAGCAGAC ATGGCACATC
    CAAAGCAATA CACAACCTCC TCAGGCTCCA GTTCCACCCC TAGGATATGT ATCTGGAAAC
    CTTATTTCAG ACTTGGAAAC AGACGTTCCA GATGATGATG AGGATGATAT TGAAGAAGAA
    ACTCTAGAAA TCAATAGGCC ACTGAGAGGT CTAGAGCATA CTCCAGGCTC CAGTATGGAC
    AATCTAGACA GCTCTGTGAC AGGTTCAATG GTAAATGGAT GGGGTTCTGC GTCTGATGAG
    GACCGTAACT TTTCTAGTCA TAGATCTAGT GTAGGTAGCT CCTCAGATGA CTCGATCTTT
    ACCAGCGGCA GTTTTGCACA AGCACTGGTT GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG
    GATGGAACCA GCCTGACAAG AACAGGCAAA GCATATCCTT CCTCTCAGAG ACCTCGGCCA
    AGCAGTCCTT TTTCTACTGA CAGCAACACC AGTGCAGCTG TCACTCAGAG TCAGAGGCCA
    AGGCCCACTA AAAAACACAA GGGAGGCCGG TTGGACCAAC CCCCCTTGCC TCATCGTAGG
    GAAGGAATAA CAGACGATCT TCCACCACCA CCTGACCCAC CGCCTGGTCA GGGTTTAAGG
    CAGCAAATAG GCTCGGGCCA GCGCGGAGGC TCGGCAGAGA GGAAAGGAAG CTCTCTTGAG
    AGGCAGCATG CACCCAGCGT AGATGACACA AAGAGCTCCT TGGACCGGCA AGCTAGGACG
    TCACTAGAGT GGCAGCGGCA AACCCAAGAG TGGATAAGCT CTGCAGAACG CCAAGAGGAT
    TTCAGGAAAG CCCAACACAA GCAAGCATTC GGATCAGAAG AGGCACTGGT ACCATACAGT
    AAACCCAACT TCCCATCCCC TGGTGGCCAC AGCTCTTCAG GAACAGCTTC TTCTAAAGGA
    TCGACTGGAC CTAGAAAAGG TGAAGTCTTT CGAGGAGGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGATG TAGGCTATTT GGGATCAAAC AGTCAAGGAC AGTTTACTGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154018.1
    CDS415..5214
    Translation

    Target ORF information:

    RefSeq Version XM_015298532.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT TCAGTCCTTC
    ACCTTCACCC CTGCAGTCAC ATTCCAAAGA GGAGATGGAG GACTAATGAG CAATGGAAGT
    CGACCAGGTT TGTTGAATGC AAGTGACCCC AGTTATCCTT GGCTTGCAGA CTCTTGGCCT
    GCCACAAGTC TGCCAGTTAA CAACAGCTCT AGTGGACCCA ATGACCTTGC CAATTTCAGT
    CGTGGAGATG TGTTGCCACC AGTGCCAGGA CAAGGAGATA AAACTGCTAC TATGCTTTCT
    GATGGAGCCA TTTACAGCAG CATTGACTTC ACGACAAAAA CTAGTTACAA CAGTTCCAGC
    CAAATAACGC AAGCTACACC ATATGCCACC ACCCAGATAC TGCATTCCAA CAGCATCCAT
    GAGTTGGCAG TCGACTTACC TGATCCTCAG TGGAAAAGCT CAATTCAGCA AAAAAATGAC
    CTGATGGGAT TTGGCTACTC TCTCCCAGAC CAAGGCAAAG GCAACAATGC CTTGCTTTAC
    ATCCCTGACT ACCGCGTGGC TGAGGGATTG GCTAATAGAT TGCCACACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC CCACAACAGC TCCGAAAGGA GTGGCAGCCT CTCAGGTGGC
    AAAGGAGGGA AAAAGAAGAA AAGCAAAAAT ACTGCCAAGC CTCAGAAAAG TAATGGTTCA
    AACTGGGCCA GTGTTCCCCT CCCACCCCCT CCTGTGCAGC CGCTTCCAGG CACAGAGCTG
    GAACACTACG GGGTGGATCA ACAAGAAGCA GGATATGACA GTGATGGTTG GTGTCCACCC
    TTGCCTGTTC AGACCTACCT CCATCAGGGC ATGGAGGATG AGCTTGAAGA AGATGATGAT
    CGTGTTCCCA CACCTCCTGT GCGAGGAGTA GCTTCATCAC CTGCGATCTC CTTTGGGCAA
    CAATCAACTG CAACCCTCAC GCCTTCTCCG CGAGAGGAGA TGCAGCCAAT GCTTCAAGCC
    CACCTCGATG AATTAACCAG GGCCTACCAG TTTGATATAG CAAAGCAGAC ATGGCACATC
    CAAAGCAATA CACAACCTCC TCAGGCTCCA GTTCCACCCC TAGGATATGT ATCTGGAAAC
    CTTATTTCAG ACTTGGAAAC AGACGTTCCA GATGATGATG AGGATGATAT TGAAGAAGAA
    ACTCTAGAAA TCAATAGGCC ACTGAGAGGT CTAGAGCATA CTCCAGGCTC CAGTATGGAC
    AATCTAGACA GCTCTGTGAC AGGTTCAATG GTAAATGGAT GGGGTTCTGC GTCTGATGAG
    GACCGTAACT TTTCTAGTCA TAGATCTAGT GTAGGTAGCT CCTCAGATGA CTCGATCTTT
    ACCAGCGGCA GTTTTGCACA AGCACTGGTT GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG
    GATGGAACCA GCCTGACAAG AACAGGCAAA GCATATCCTT CCTCTCAGAG ACCTCGGCCA
    AGCAGTCCTT TTTCTACTGA CAGCAACACC AGTGCAGCTG TCACTCAGAG TCAGAGGCCA
    AGGCCCACTA AAAAACACAA GGGAGGCCGG TTGGACCAAC CCCCCTTGCC TCATCGTAGG
    GAAGGAATAA CAGACGATCT TCCACCACCA CCTGACCCAC CGCCTGGTCA GGGTTTAAGG
    CAGCAAATAG GCTCGGGCCA GCGCGGAGGC TCGGCAGAGA GGAAAGGAAG CTCTCTTGAG
    AGGCAGCATG CACCCAGCGT AGATGACACA AAGAGCTCCT TGGACCGGCA AGCTAGGACG
    TCACTAGAGT GGCAGCGGCA AACCCAAGAG TGGATAAGCT CTGCAGAACG CCAAGAGGAT
    TTCAGGAAAG CCCAACACAA GCAAGCATTC GGATCAGAAG AGGCACTGGT ACCATACAGT
    AAACCCAACT TCCCATCCCC TGGTGGCCAC AGCTCTTCAG GAACAGCTTC TTCTAAAGGA
    TCGACTGGAC CTAGAAAAGG TGAAGTCTTT CGAGGAGGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGATG TAGGCTATTT GGGATCAAAC AGTCAAGGAC AGTTTACTGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30209
    Clone ID Related Accession (Same CDS sequence) XM_015298532.1 , XM_015298532.2
    Accession Version XM_015298532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT TCAGTCCTTC
    ACCTTCACCC CTGCAGTCAC ATTCCAAAGA GGAGATGGAG GACTAATGAG CAATGGAAGT
    CGACCAGGTT TGTTGAATGC AAGTGACCCC AGTTATCCTT GGCTTGCAGA CTCTTGGCCT
    GCCACAAGTC TGCCAGTTAA CAACAGCTCT AGTGGACCCA ATGACCTTGC CAATTTCAGT
    CGTGGAGATG TGTTGCCACC AGTGCCAGGA CAAGGAGATA AAACTGCTAC TATGCTTTCT
    GATGGAGCCA TTTACAGCAG CATTGACTTC ACGACAAAAA CTAGTTACAA CAGTTCCAGC
    CAAATAACGC AAGCTACACC ATATGCCACC ACCCAGATAC TGCATTCCAA CAGCATCCAT
    GAGTTGGCAG TCGACTTACC TGATCCTCAG TGGAAAAGCT CAATTCAGCA AAAAAATGAC
    CTGATGGGAT TTGGCTACTC TCTCCCAGAC CAAGGCAAAG GCAACAATGC CTTGCTTTAC
    ATCCCTGACT ACCGCGTGGC TGAGGGATTG GCTAATAGAT TGCCACACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC CCACAACAGC TCCGAAAGGA GTGGCAGCCT CTCAGGTGGC
    AAAGGAGGGA AAAAGAAGAA AAGCAAAAAT ACTGCCAAGC CTCAGAAAAG TAATGGTTCA
    AACTGGGCCA GTGTTCCCCT CCCACCCCCT CCTGTGCAGC CGCTTCCAGG CACAGAGCTG
    GAACACTACG GGGTGGATCA ACAAGAAGCA GGATATGACA GTGATGGTTG GTGTCCACCC
    TTGCCTGTTC AGACCTACCT CCATCAGGGC ATGGAGGATG AGCTTGAAGA AGATGATGAT
    CGTGTTCCCA CACCTCCTGT GCGAGGAGTA GCTTCATCAC CTGCGATCTC CTTTGGGCAA
    CAATCAACTG CAACCCTCAC GCCTTCTCCG CGAGAGGAGA TGCAGCCAAT GCTTCAAGCC
    CACCTCGATG AATTAACCAG GGCCTACCAG TTTGATATAG CAAAGCAGAC ATGGCACATC
    CAAAGCAATA CACAACCTCC TCAGGCTCCA GTTCCACCCC TAGGATATGT ATCTGGAAAC
    CTTATTTCAG ACTTGGAAAC AGACGTTCCA GATGATGATG AGGATGATAT TGAAGAAGAA
    ACTCTAGAAA TCAATAGGCC ACTGAGAGGT CTAGAGCATA CTCCAGGCTC CAGTATGGAC
    AATCTAGACA GCTCTGTGAC AGGTTCAATG GTAAATGGAT GGGGTTCTGC GTCTGATGAG
    GACCGTAACT TTTCTAGTCA TAGATCTAGT GTAGGTAGCT CCTCAGATGA CTCGATCTTT
    ACCAGCGGCA GTTTTGCACA AGCACTGGTT GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG
    GATGGAACCA GCCTGACAAG AACAGGCAAA GCATATCCTT CCTCTCAGAG ACCTCGGCCA
    AGCAGTCCTT TTTCTACTGA CAGCAACACC AGTGCAGCTG TCACTCAGAG TCAGAGGCCA
    AGGCCCACTA AAAAACACAA GGGAGGCCGG TTGGACCAAC CCCCCTTGCC TCATCGTAGG
    GAAGGAATAA CAGACGATCT TCCACCACCA CCTGACCCAC CGCCTGGTCA GGGTTTAAGG
    CAGCAAATAG GCTCGGGCCA GCGCGGAGGC TCGGCAGAGA GGAAAGGAAG CTCTCTTGAG
    AGGCAGCATG CACCCAGCGT AGATGACACA AAGAGCTCCT TGGACCGGCA AGCTAGGACG
    TCACTAGAGT GGCAGCGGCA AACCCAAGAG TGGATAAGCT CTGCAGAACG CCAAGAGGAT
    TTCAGGAAAG CCCAACACAA GCAAGCATTC GGATCAGAAG AGGCACTGGT ACCATACAGT
    AAACCCAACT TCCCATCCCC TGGTGGCCAC AGCTCTTCAG GAACAGCTTC TTCTAAAGGA
    TCGACTGGAC CTAGAAAAGG TGAAGTCTTT CGAGGAGGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGATG TAGGCTATTT GGGATCAAAC AGTCAAGGAC AGTTTACTGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154018.1
    CDS381..5180
    Translation

    Target ORF information:

    RefSeq Version XM_015298532.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC CCCCACAGTT TGTGGTGAGA
    CCACGAGATC AGATTGTAGC CCAGGGTCGA ACTGTGACTT TTCCTTGTGA AACTAAAGGA
    AATCCCCAGC CAGCTGTTTT TTGGCAAAAA GAAGGAAGCC AGAATCTGCT CTTCCCAAAC
    CAGCCTCTCC AACCCAACAG TAGATACTCA GTATCACCAA CTGGAGACCT CACTATTACC
    AACATTCAAC GGTCTGATGC AGGCTACTAC ATTTGTCAAG CACTGACGGT TGCTGGAAGC
    ATTTTAGCAA AGGCTCAGCT GGAGGTTACT GATGTCTTGA CAGATAGACC TCCACCCATC
    ATCCTCCAGG GGCCTGTCAA TCAGACGCTG GCAGTGGACG GAACAGCTCT GCTGAAGTGC
    AAGGCTACTG GTGACCCACT TCCTGTTATC AGCTGGTTAA AAGAAGGCTT CACTTTTCTG
    GGCAGAGACC CTCGAACATC CATACAGGAT CAGGGGACCC TTCAGATTAA AACTCTGCGG
    CTGTCTGATA CTGGAACTTA CACTTGTGTT GCAACAAGTT CAAGTGGGGA GACATCATGG
    AGTGCTGTTC TGGATGTGAC AGAGTTGGGT GGAGCAACAG TCAGTAAAAA TTATGACATA
    AATGACCTCC CTGGACCACC ATCCAAACCT CAGGTCACTG ATGTTACTAA GAACAGTGTC
    ACCCTGTCCT GGCAACCAGG GACCCCTGGA AGCCTACCAG CTAGTGCATA TATCATTGAG
    GCTTTTAGTC AATCTGTGAG CAATAGTTGG CAAACAGTGG CTAACCATGT GAAGACCACT
    CTCTACACTG TGAGAGGACT GCGTCCCAAT ACCATCTATC TGTTTATGGT GAGAGCTATC
    AATTCACAAG GTCTGAGTGA TCCCAGCCCT ATGTCAGATC CTGTTCGAAC ACAAGATATC
    AGTCCACCAG CACAAGGTGT GGATCACAGG CAAGTCCAGA AGGAACTGGG AGACGTCATT
    GTACGACTGC ATAACCCTGT TGTGCTGACA CCCACAACAG TTCAAGTTAC CTGGACAGTT
    GACCGTCAAT CGCAGTTTAT TCAGGGCTAC CGAGTAATGT ATCGCCAAAC GTCTGGCCTA
    CCGTCTCCAG CTGTATGGCA GAATTTGGAG GTCAAAGTCC CAACAGAGCG CAGTGCTGTT
    CTAGTCAACT TGAAAAAAGG AGTCACTTAT GAAATTAAAG TTCGCCCGTA CTTCAATGAA
    TTTCAAGGAA TGGACAGTGA ATCAAAGTCT GCTCGTACCA CAGAGGAAGC TCCAAGTGCC
    CCTCCTCAGT CTGTTACTGT CCTGACAGTT GGAAATCACA ACAGCACAAG CATCAGCATC
    TCCTGGGATC CTCCCCCTCC AGATCACCAA AATGGAATCA TCCAGGAGTA CAAGATCTGG
    TGTCTAGGTA ATGAGACTCG ATTTCATATC AACAAAACAG TAGATGCAGC CATTCGGTCT
    GTAGTAATAG GTGGCCTGTA TCCAGGTATT CAGTACCGTG TAGAAGTTGC TGCCAGCACC
    AGTGCAGGTG TGGGAGTGAA AAGTGAGCCA CAACCCATAA TAATTGGAGG CCGTAACGAA
    GTTGTCATCA CTGAAAATAA CAACAGCATA ACTGAGCAAA TCACTGATGT TGTCAAACAG
    CCTGCCTTTA TAGCTGGCAT CGGTGGTGCA TGTTGGGTAA TTCTGATGGG TTTCAGCATC
    TGGCTGTACT GGCGCAGAAA GAAGAGGAAG GGGCTCAGTA ATTATGCTGT TCAGTCCTTC
    ACCTTCACCC CTGCAGTCAC ATTCCAAAGA GGAGATGGAG GACTAATGAG CAATGGAAGT
    CGACCAGGTT TGTTGAATGC AAGTGACCCC AGTTATCCTT GGCTTGCAGA CTCTTGGCCT
    GCCACAAGTC TGCCAGTTAA CAACAGCTCT AGTGGACCCA ATGACCTTGC CAATTTCAGT
    CGTGGAGATG TGTTGCCACC AGTGCCAGGA CAAGGAGATA AAACTGCTAC TATGCTTTCT
    GATGGAGCCA TTTACAGCAG CATTGACTTC ACGACAAAAA CTAGTTACAA CAGTTCCAGC
    CAAATAACGC AAGCTACACC ATATGCCACC ACCCAGATAC TGCATTCCAA CAGCATCCAT
    GAGTTGGCAG TCGACTTACC TGATCCTCAG TGGAAAAGCT CAATTCAGCA AAAAAATGAC
    CTGATGGGAT TTGGCTACTC TCTCCCAGAC CAAGGCAAAG GCAACAATGC CTTGCTTTAC
    ATCCCTGACT ACCGCGTGGC TGAGGGATTG GCTAATAGAT TGCCACACAA CCAGTCACAG
    GATTTCAGCA CCACCAGCTC CCACAACAGC TCCGAAAGGA GTGGCAGCCT CTCAGGTGGC
    AAAGGAGGGA AAAAGAAGAA AAGCAAAAAT ACTGCCAAGC CTCAGAAAAG TAATGGTTCA
    AACTGGGCCA GTGTTCCCCT CCCACCCCCT CCTGTGCAGC CGCTTCCAGG CACAGAGCTG
    GAACACTACG GGGTGGATCA ACAAGAAGCA GGATATGACA GTGATGGTTG GTGTCCACCC
    TTGCCTGTTC AGACCTACCT CCATCAGGGC ATGGAGGATG AGCTTGAAGA AGATGATGAT
    CGTGTTCCCA CACCTCCTGT GCGAGGAGTA GCTTCATCAC CTGCGATCTC CTTTGGGCAA
    CAATCAACTG CAACCCTCAC GCCTTCTCCG CGAGAGGAGA TGCAGCCAAT GCTTCAAGCC
    CACCTCGATG AATTAACCAG GGCCTACCAG TTTGATATAG CAAAGCAGAC ATGGCACATC
    CAAAGCAATA CACAACCTCC TCAGGCTCCA GTTCCACCCC TAGGATATGT ATCTGGAAAC
    CTTATTTCAG ACTTGGAAAC AGACGTTCCA GATGATGATG AGGATGATAT TGAAGAAGAA
    ACTCTAGAAA TCAATAGGCC ACTGAGAGGT CTAGAGCATA CTCCAGGCTC CAGTATGGAC
    AATCTAGACA GCTCTGTGAC AGGTTCAATG GTAAATGGAT GGGGTTCTGC GTCTGATGAG
    GACCGTAACT TTTCTAGTCA TAGATCTAGT GTAGGTAGCT CCTCAGATGA CTCGATCTTT
    ACCAGCGGCA GTTTTGCACA AGCACTGGTT GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG
    GATGGAACCA GCCTGACAAG AACAGGCAAA GCATATCCTT CCTCTCAGAG ACCTCGGCCA
    AGCAGTCCTT TTTCTACTGA CAGCAACACC AGTGCAGCTG TCACTCAGAG TCAGAGGCCA
    AGGCCCACTA AAAAACACAA GGGAGGCCGG TTGGACCAAC CCCCCTTGCC TCATCGTAGG
    GAAGGAATAA CAGACGATCT TCCACCACCA CCTGACCCAC CGCCTGGTCA GGGTTTAAGG
    CAGCAAATAG GCTCGGGCCA GCGCGGAGGC TCGGCAGAGA GGAAAGGAAG CTCTCTTGAG
    AGGCAGCATG CACCCAGCGT AGATGACACA AAGAGCTCCT TGGACCGGCA AGCTAGGACG
    TCACTAGAGT GGCAGCGGCA AACCCAAGAG TGGATAAGCT CTGCAGAACG CCAAGAGGAT
    TTCAGGAAAG CCCAACACAA GCAAGCATTC GGATCAGAAG AGGCACTGGT ACCATACAGT
    AAACCCAACT TCCCATCCCC TGGTGGCCAC AGCTCTTCAG GAACAGCTTC TTCTAAAGGA
    TCGACTGGAC CTAGAAAAGG TGAAGTCTTT CGAGGAGGTC ACCAACGCAA TGCCAGTGAC
    CTTCTTGATG TAGGCTATTT GGGATCAAAC AGTCAAGGAC AGTTTACTGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30210
    Clone ID Related Accession (Same CDS sequence) XM_015298535.1 , XM_015298535.2
    Accession Version XM_015298535.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGCCTTGC TTTACATCCC TGACTACCGC
    GTGGCTGAGG GATTGGCTAA TAGATTGCCA CACAACCAGT CACAGGATTT CAGCACCACC
    AGCTCCCACA ACAGCTCCGA AAGGAGTGGC AGCCTCTCAG GTGGCAAAGG AGGGAAAAAG
    AAGAAAAGCA AAAATACTGC CAAGCCTCAG AAAAGTAATG GTTCAAACTG GGCCAGTGTT
    CCCCTCCCAC CCCCTCCTGT GCAGCCGCTT CCAGGCACAG AGCTGGAACA CTACGGGGTG
    GATCAACAAG AAGCAGGATA TGACAGTGAT GGTTGGTGTC CACCCTTGCC TGTTCAGACC
    TACCTCCATC AGGGCATGGA GGATGAGCTT GAAGAAGATG ATGATCGTGT TCCCACACCT
    CCTGTGCGAG GAGTAGCTTC ATCACCTGCG ATCTCCTTTG GGCAACAATC AACTGCAACC
    CTCACGCCTT CTCCGCGAGA GGAGATGCAG CCAATGCTTC AAGCCCACCT CGATGAATTA
    ACCAGGGCCT ACCAGTTTGA TATAGCAAAG CAGACATGGC ACATCCAAAG CAATACACAA
    CCTCCTCAGG CTCCAGTTCC ACCCCTAGGA TATGTATCTG GAAACCTTAT TTCAGACTTG
    GAAACAGACG TTCCAGATGA TGATGAGGAT GATATTGAAG AAGAAACTCT AGAAATCAAT
    AGGCCACTGA GAGGTCTAGA GCATACTCCA GGCTCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGATGGGGT TCTGCGTCTG ATGAGGACCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGACTCGA TCTTTACCAG CGGCAGTTTT
    GCACAAGCAC TGGTTGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTG
    ACAAGAACAG GCAAAGCATA TCCTTCCTCT CAGAGACCTC GGCCAAGCAG TCCTTTTTCT
    ACTGACAGCA ACACCAGTGC AGCTGTCACT CAGAGTCAGA GGCCAAGGCC CACTAAAAAA
    CACAAGGGAG GCCGGTTGGA CCAACCCCCC TTGCCTCATC GTAGGGAAGG AATAACAGAC
    GATCTTCCAC CACCACCTGA CCCACCGCCT GGTCAGGGTT TAAGGCAGCA AATAGGCTCG
    GGCCAGCGCG GAGGCTCGGC AGAGAGGAAA GGAAGCTCTC TTGAGAGGCA GCATGCACCC
    AGCGTAGATG ACACAAAGAG CTCCTTGGAC CGGCAAGCTA GGACGTCACT AGAGTGGCAG
    CGGCAAACCC AAGAGTGGAT AAGCTCTGCA GAACGCCAAG AGGATTTCAG GAAAGCCCAA
    CACAAGCAAG CATTCGGATC AGAAGAGGCA CTGGTACCAT ACAGTAAACC CAACTTCCCA
    TCCCCTGGTG GCCACAGCTC TTCAGGAACA GCTTCTTCTA AAGGATCGAC TGGACCTAGA
    AAAGGTGAAG TCTTTCGAGG AGGTCACCAA CGCAATGCCA GTGACCTTCT TGATGTAGGC
    TATTTGGGAT CAAACAGTCA AGGACAGTTT ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154021.1
    CDS408..5192
    Translation

    Target ORF information:

    RefSeq Version XM_015298535.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGCCTTGC TTTACATCCC TGACTACCGC
    GTGGCTGAGG GATTGGCTAA TAGATTGCCA CACAACCAGT CACAGGATTT CAGCACCACC
    AGCTCCCACA ACAGCTCCGA AAGGAGTGGC AGCCTCTCAG GTGGCAAAGG AGGGAAAAAG
    AAGAAAAGCA AAAATACTGC CAAGCCTCAG AAAAGTAATG GTTCAAACTG GGCCAGTGTT
    CCCCTCCCAC CCCCTCCTGT GCAGCCGCTT CCAGGCACAG AGCTGGAACA CTACGGGGTG
    GATCAACAAG AAGCAGGATA TGACAGTGAT GGTTGGTGTC CACCCTTGCC TGTTCAGACC
    TACCTCCATC AGGGCATGGA GGATGAGCTT GAAGAAGATG ATGATCGTGT TCCCACACCT
    CCTGTGCGAG GAGTAGCTTC ATCACCTGCG ATCTCCTTTG GGCAACAATC AACTGCAACC
    CTCACGCCTT CTCCGCGAGA GGAGATGCAG CCAATGCTTC AAGCCCACCT CGATGAATTA
    ACCAGGGCCT ACCAGTTTGA TATAGCAAAG CAGACATGGC ACATCCAAAG CAATACACAA
    CCTCCTCAGG CTCCAGTTCC ACCCCTAGGA TATGTATCTG GAAACCTTAT TTCAGACTTG
    GAAACAGACG TTCCAGATGA TGATGAGGAT GATATTGAAG AAGAAACTCT AGAAATCAAT
    AGGCCACTGA GAGGTCTAGA GCATACTCCA GGCTCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGATGGGGT TCTGCGTCTG ATGAGGACCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGACTCGA TCTTTACCAG CGGCAGTTTT
    GCACAAGCAC TGGTTGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTG
    ACAAGAACAG GCAAAGCATA TCCTTCCTCT CAGAGACCTC GGCCAAGCAG TCCTTTTTCT
    ACTGACAGCA ACACCAGTGC AGCTGTCACT CAGAGTCAGA GGCCAAGGCC CACTAAAAAA
    CACAAGGGAG GCCGGTTGGA CCAACCCCCC TTGCCTCATC GTAGGGAAGG AATAACAGAC
    GATCTTCCAC CACCACCTGA CCCACCGCCT GGTCAGGGTT TAAGGCAGCA AATAGGCTCG
    GGCCAGCGCG GAGGCTCGGC AGAGAGGAAA GGAAGCTCTC TTGAGAGGCA GCATGCACCC
    AGCGTAGATG ACACAAAGAG CTCCTTGGAC CGGCAAGCTA GGACGTCACT AGAGTGGCAG
    CGGCAAACCC AAGAGTGGAT AAGCTCTGCA GAACGCCAAG AGGATTTCAG GAAAGCCCAA
    CACAAGCAAG CATTCGGATC AGAAGAGGCA CTGGTACCAT ACAGTAAACC CAACTTCCCA
    TCCCCTGGTG GCCACAGCTC TTCAGGAACA GCTTCTTCTA AAGGATCGAC TGGACCTAGA
    AAAGGTGAAG TCTTTCGAGG AGGTCACCAA CGCAATGCCA GTGACCTTCT TGATGTAGGC
    TATTTGGGAT CAAACAGTCA AGGACAGTTT ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30210
    Clone ID Related Accession (Same CDS sequence) XM_015298535.1 , XM_015298535.2
    Accession Version XM_015298535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGCCTTGC TTTACATCCC TGACTACCGC
    GTGGCTGAGG GATTGGCTAA TAGATTGCCA CACAACCAGT CACAGGATTT CAGCACCACC
    AGCTCCCACA ACAGCTCCGA AAGGAGTGGC AGCCTCTCAG GTGGCAAAGG AGGGAAAAAG
    AAGAAAAGCA AAAATACTGC CAAGCCTCAG AAAAGTAATG GTTCAAACTG GGCCAGTGTT
    CCCCTCCCAC CCCCTCCTGT GCAGCCGCTT CCAGGCACAG AGCTGGAACA CTACGGGGTG
    GATCAACAAG AAGCAGGATA TGACAGTGAT GGTTGGTGTC CACCCTTGCC TGTTCAGACC
    TACCTCCATC AGGGCATGGA GGATGAGCTT GAAGAAGATG ATGATCGTGT TCCCACACCT
    CCTGTGCGAG GAGTAGCTTC ATCACCTGCG ATCTCCTTTG GGCAACAATC AACTGCAACC
    CTCACGCCTT CTCCGCGAGA GGAGATGCAG CCAATGCTTC AAGCCCACCT CGATGAATTA
    ACCAGGGCCT ACCAGTTTGA TATAGCAAAG CAGACATGGC ACATCCAAAG CAATACACAA
    CCTCCTCAGG CTCCAGTTCC ACCCCTAGGA TATGTATCTG GAAACCTTAT TTCAGACTTG
    GAAACAGACG TTCCAGATGA TGATGAGGAT GATATTGAAG AAGAAACTCT AGAAATCAAT
    AGGCCACTGA GAGGTCTAGA GCATACTCCA GGCTCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGATGGGGT TCTGCGTCTG ATGAGGACCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGACTCGA TCTTTACCAG CGGCAGTTTT
    GCACAAGCAC TGGTTGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTG
    ACAAGAACAG GCAAAGCATA TCCTTCCTCT CAGAGACCTC GGCCAAGCAG TCCTTTTTCT
    ACTGACAGCA ACACCAGTGC AGCTGTCACT CAGAGTCAGA GGCCAAGGCC CACTAAAAAA
    CACAAGGGAG GCCGGTTGGA CCAACCCCCC TTGCCTCATC GTAGGGAAGG AATAACAGAC
    GATCTTCCAC CACCACCTGA CCCACCGCCT GGTCAGGGTT TAAGGCAGCA AATAGGCTCG
    GGCCAGCGCG GAGGCTCGGC AGAGAGGAAA GGAAGCTCTC TTGAGAGGCA GCATGCACCC
    AGCGTAGATG ACACAAAGAG CTCCTTGGAC CGGCAAGCTA GGACGTCACT AGAGTGGCAG
    CGGCAAACCC AAGAGTGGAT AAGCTCTGCA GAACGCCAAG AGGATTTCAG GAAAGCCCAA
    CACAAGCAAG CATTCGGATC AGAAGAGGCA CTGGTACCAT ACAGTAAACC CAACTTCCCA
    TCCCCTGGTG GCCACAGCTC TTCAGGAACA GCTTCTTCTA AAGGATCGAC TGGACCTAGA
    AAAGGTGAAG TCTTTCGAGG AGGTCACCAA CGCAATGCCA GTGACCTTCT TGATGTAGGC
    TATTTGGGAT CAAACAGTCA AGGACAGTTT ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154021.1
    CDS376..5160
    Translation

    Target ORF information:

    RefSeq Version XM_015298535.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTCACATTCC AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGTCGACC AGGTTTGTTG
    AATGCAAGTG ACCCCAGTTA TCCTTGGCTT GCAGACTCTT GGCCTGCCAC AAGTCTGCCA
    GTTAACAACA GCTCTAGTGG ACCCAATGAC CTTGCCAATT TCAGTCGTGG AGATGTGTTG
    CCACCAGTGC CAGGACAAGG AGATAAAACT GCTACTATGC TTTCTGATGG AGCCATTTAC
    AGCAGCATTG ACTTCACGAC AAAAACTAGT TACAACAGTT CCAGCCAAAT AACGCAAGCT
    ACACCATATG CCACCACCCA GATACTGCAT TCCAACAGCA TCCATGAGTT GGCAGTCGAC
    TTACCTGATC CTCAGTGGAA AAGCTCAATT CAGCAAAAAA ATGACCTGAT GGGATTTGGC
    TACTCTCTCC CAGACCAAGG CAAAGGCAAC AATGCCTTGC TTTACATCCC TGACTACCGC
    GTGGCTGAGG GATTGGCTAA TAGATTGCCA CACAACCAGT CACAGGATTT CAGCACCACC
    AGCTCCCACA ACAGCTCCGA AAGGAGTGGC AGCCTCTCAG GTGGCAAAGG AGGGAAAAAG
    AAGAAAAGCA AAAATACTGC CAAGCCTCAG AAAAGTAATG GTTCAAACTG GGCCAGTGTT
    CCCCTCCCAC CCCCTCCTGT GCAGCCGCTT CCAGGCACAG AGCTGGAACA CTACGGGGTG
    GATCAACAAG AAGCAGGATA TGACAGTGAT GGTTGGTGTC CACCCTTGCC TGTTCAGACC
    TACCTCCATC AGGGCATGGA GGATGAGCTT GAAGAAGATG ATGATCGTGT TCCCACACCT
    CCTGTGCGAG GAGTAGCTTC ATCACCTGCG ATCTCCTTTG GGCAACAATC AACTGCAACC
    CTCACGCCTT CTCCGCGAGA GGAGATGCAG CCAATGCTTC AAGCCCACCT CGATGAATTA
    ACCAGGGCCT ACCAGTTTGA TATAGCAAAG CAGACATGGC ACATCCAAAG CAATACACAA
    CCTCCTCAGG CTCCAGTTCC ACCCCTAGGA TATGTATCTG GAAACCTTAT TTCAGACTTG
    GAAACAGACG TTCCAGATGA TGATGAGGAT GATATTGAAG AAGAAACTCT AGAAATCAAT
    AGGCCACTGA GAGGTCTAGA GCATACTCCA GGCTCCAGTA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGATGGGGT TCTGCGTCTG ATGAGGACCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGACTCGA TCTTTACCAG CGGCAGTTTT
    GCACAAGCAC TGGTTGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTG
    ACAAGAACAG GCAAAGCATA TCCTTCCTCT CAGAGACCTC GGCCAAGCAG TCCTTTTTCT
    ACTGACAGCA ACACCAGTGC AGCTGTCACT CAGAGTCAGA GGCCAAGGCC CACTAAAAAA
    CACAAGGGAG GCCGGTTGGA CCAACCCCCC TTGCCTCATC GTAGGGAAGG AATAACAGAC
    GATCTTCCAC CACCACCTGA CCCACCGCCT GGTCAGGGTT TAAGGCAGCA AATAGGCTCG
    GGCCAGCGCG GAGGCTCGGC AGAGAGGAAA GGAAGCTCTC TTGAGAGGCA GCATGCACCC
    AGCGTAGATG ACACAAAGAG CTCCTTGGAC CGGCAAGCTA GGACGTCACT AGAGTGGCAG
    CGGCAAACCC AAGAGTGGAT AAGCTCTGCA GAACGCCAAG AGGATTTCAG GAAAGCCCAA
    CACAAGCAAG CATTCGGATC AGAAGAGGCA CTGGTACCAT ACAGTAAACC CAACTTCCCA
    TCCCCTGGTG GCCACAGCTC TTCAGGAACA GCTTCTTCTA AAGGATCGAC TGGACCTAGA
    AAAGGTGAAG TCTTTCGAGG AGGTCACCAA CGCAATGCCA GTGACCTTCT TGATGTAGGC
    TATTTGGGAT CAAACAGTCA AGGACAGTTT ACTGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30211
    Clone ID Related Accession (Same CDS sequence) XM_015298540.1 , XM_015298540.2
    Accession Version XM_015298540.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGTCTGC TGACGCCACT CCTGCTCTTT GGATTTGCAT TTTTTCAAGT CTACGGATCC 
    CGTCTCCGCC AGGAGGACTT TCCACCGCGG ATTGTTGAGC ATCCTTCAGA TGTGATAGTC
    TCTAAAGGAG AACCAACAAC CCTGAACTGT AAAGCTGAAG GACGGCCAAC TCCTACTATT
    GAATGGTACA AAGATGGAGA GCGAGTGGAA ACTGACAAAG ATGATCCTCG CTCCCACCGT
    ATGCTGCTGC CTAGCGGATC TTTATTTTTC TTACGTATTG TGCATGGACG CAGGAGTAAA
    CCTGACGAAG GAAGTTATGT TTGCGTAGCA AGGAACTATC TTGGAGAAGC TGTGAGTCGC
    AATGCATCTC TGGAAGTGGC ATTGCTACGA GATGACTTCC GCCAAAACCC TACAGATGTT
    GTAGTGGCAG CTGGAGAACC TGCAATACTG GAGTGTCAGC CACCTCGTGG ACACCCTGAG
    CCTACCATCT ACTGGAAAAA AGATAAAGTC CGTATTGATG ACAGGGAAGA GCGGATCAGT
    ATACGTGGTG GGAAGCTAAT GATCTCCAAC ACACGAAAAA GTGATGCTGG CATGTACACT
    TGCGTTGGAA CCAACATGGT GGGGGAACGA GACAGTGATC CAGCAGAGCT GACTGTCTTT
    GAGCGGCCCA CGTTTCTCAG GAGACCAATC AACCAGGTGG TGCTGGAGGA GGAGGCCGTG
    GATTTCCGGT GCCAGGTGCA GGGGGACCCG CAGCCGACCG TCCGCTGGAA GAAGGATGAC
    GCAGACCTGC CAAGAGGAAG GTATGACATT AAAGATGACT ACACTTTGCG CATCAAGAAG
    GCTATGAGCA CAGATGAAGG CACCTACACG TGCATTGCTG AGAATCGAGT TGGAAAAGTG
    GAAGCATCTG CTACCCTTAC TGTGCGAGCT CGCCCCGTTG CTCCCCCACA GTTTGTGGTG
    AGACCACGAG ATCAGATTGT AGCCCAGGGT CGAACTGTGA CTTTTCCTTG TGAAACTAAA
    GGAAATCCCC AGCCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATCT GCTCTTCCCA
    AACCAGCCTC TCCAACCCAA CAGTAGATAC TCAGTATCAC CAACTGGAGA CCTCACTATT
    ACCAACATTC AACGGTCTGA TGCAGGCTAC TACATTTGTC AAGCACTGAC GGTTGCTGGA
    AGCATTTTAG CAAAGGCTCA GCTGGAGGTT ACTGATGTCT TGACAGATAG ACCTCCACCC
    ATCATCCTCC AGGGGCCTGT CAATCAGACG CTGGCAGTGG ACGGAACAGC TCTGCTGAAG
    TGCAAGGCTA CTGGTGACCC ACTTCCTGTT ATCAGCTGGT TAAAAGAAGG CTTCACTTTT
    CTGGGCAGAG ACCCTCGAAC ATCCATACAG GATCAGGGGA CCCTTCAGAT TAAAACTCTG
    CGGCTGTCTG ATACTGGAAC TTACACTTGT GTTGCAACAA GTTCAAGTGG GGAGACATCA
    TGGAGTGCTG TTCTGGATGT GACAGAGTTG GGTGGAGCAA CAGTCAGTAA AAATTATGAC
    ATAAATGACC TCCCTGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCCTGT CCTGGCAACC AGGGACCCCT GGAAGCCTAC CAGCTAGTGC ATATATCATT
    GAGGCTTTTA GTCAATCTGT GAGCAATAGT TGGCAAACAG TGGCTAACCA TGTGAAGACC
    ACTCTCTACA CTGTGAGAGG ACTGCGTCCC AATACCATCT ATCTGTTTAT GGTGAGAGCT
    ATCAATTCAC AAGGTCTGAG TGATCCCAGC CCTATGTCAG ATCCTGTTCG AACACAAGAT
    ATCAGTCCAC CAGCACAAGG TGTGGATCAC AGGCAAGTCC AGAAGGAACT GGGAGACGTC
    ATTGTACGAC TGCATAACCC TGTTGTGCTG ACACCCACAA CAGTTCAAGT TACCTGGACA
    GTTGACCGTC AATCGCAGTT TATTCAGGGC TACCGAGTAA TGTATCGCCA AACGTCTGGC
    CTACCGTCTC CAGCTGTATG GCAGAATTTG GAGGTCAAAG TCCCAACAGA GCGCAGTGCT
    GTTCTAGTCA ACTTGAAAAA AGGAGTCACT TATGAAATTA AAGTTCGCCC GTACTTCAAT
    GAATTTCAAG GAATGGACAG TGAATCAAAG TCTGCTCGTA CCACAGAGGA AGCTCCAAGT
    GCCCCTCCTC AGTCTGTTAC TGTCCTGACA GTTGGAAATC ACAACAGCAC AAGCATCAGC
    ATCTCCTGGG ATCCTCCCCC TCCAGATCAC CAAAATGGAA TCATCCAGGA GTACAAGATC
    TGGTGTCTAG GTAATGAGAC TCGATTTCAT ATCAACAAAA CAGTAGATGC AGCCATTCGG
    TCTGTAGTAA TAGGTGGCCT GTATCCAGGT ATTCAGTACC GTGTAGAAGT TGCTGCCAGC
    ACCAGTGCAG GTGTGGGAGT GAAAAGTGAG CCACAACCCA TAATAATTGG AGGCCGTAAC
    GAAGTTGTCA TCACTGAAAA TAACAACAGC ATAACTGAGC AAATCACTGA TGTTGTCAAA
    CAGCCTGCCT TTATAGCTGG CATCGGTGGT GCATGTTGGG TAATTCTGAT GGGTTTCAGC
    ATCTGGCTGT ACTGGCGCAG AAAGAAGAGG AAGGGGCTCA GTAATTATGC TGTTCAGTCC
    TTCACCTTCA CCCCTGCAGT CACATTCCAA AGAGGAGATG GAGGACTAAT GAGCAATGGA
    AGTCGACCAG GTTTGTTGAA TGCAAGTGAC CCCAGTTATC CTTGGCTTGC AGACTCTTGG
    CCTGCCACAA GTCTGCCAGT TAACAACAGC TCTAGTGGAC CCAATGACCT TGCCAATTTC
    AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA GGACAAGGAG ATAAAACTGC TACTATGCTT
    TCTGATGGAG CCATTTACAG CAGCATTGAC TTCACGACAA AAACTAGTTA CAACAGTTCC
    AGCCAAATAA CGCAAGCTAC ACCATATGCC ACCACCCAGA TACTGCATTC CAACAGCATC
    CATGAGTTGG CAGTCGACTT ACCTGATCCT CAGTGGAAAA GCTCAATTCA GCAAAAAAAT
    GACCTGATGG GATTTGGCTA CTCTCTCCCA GACCAAGGCA AAGGCAACAA TGCCTTGCTT
    TACATCCCTG ACTACCGCGT GGCTGAGGGA TTGGCTAATA GATTGCCACA CAACCAGTCA
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCCGAAA GGAGTGGCAG CCTCTCAGGT
    GGCAAAGGAG GGAAAAAGAA GAAAAGCAAA AATACTGCCA AGCCTCAGAA AAGTAATGGT
    TCAAACTGGG CCAGTGTTCC CCTCCCACCC CCTCCTGTGC AGCCGCTTCC AGGCACAGAG
    CTGGAACACT ACGGGGTGGA TCAACAAGAA GCAGGATATG ACAGTGATGG TTGGTGTCCA
    CCCTTGCCTG TTCAGACCTA CCTCCATCAG GGCATGGAGG ATGAGCTTGA AGAAGATGAT
    GATCGTGTTC CCACACCTCC TGTGCGAGGA GTAGCTTCAT CACCTGCGAT CTCCTTTGGG
    CAACAATCAA CTGCAACCCT CACGCCTTCT CCGCGAGAGG AGATGCAGCC AATGCTTCAA
    GCCCACCTCG ATGAATTAAC CAGGGCCTAC CAGTTTGATA TAGCAAAGCA GACATGGCAC
    ATCCAAAGCA ATACACAACC TCCTCAGGCT CCAGTTCCAC CCCTAGGATA TGTATCTGGA
    AACCTTATTT CAGACTTGGA AACAGACGTT CCAGATGATG ATGAGGATGA TATTGAAGAA
    GAAACTCTAG AAATCAATAG GCCACTGAGA GGTCTAGAGC ATACTCCAGG CTCCAGTATG
    GACAATCTAG ACAGCTCTGT GACAGGTTCA ATGGTAAATG GATGGGGTTC TGCGTCTGAT
    GAGGACCGTA ACTTTTCTAG TCATAGATCT AGTGTAGGTA GCTCCTCAGA TGACTCGATC
    TTTACCAGCG GCAGTTTTGC ACAAGCACTG GTTGCAGCAG CAGATAAAGC TGGTTTTAGG
    CTGGATGGAA CCAGCCTGAC AAGAACAGGC AAAGCATATC CTTCCTCTCA GAGACCTCGG
    CCAAGCAGTC CTTTTTCTAC TGACAGCAAC ACCAGTGCAG CTGTCACTCA GAGTCAGAGG
    CCAAGGCCCA CTAAAAAACA CAAGGGAGGC CGGTTGGACC AACCCCCCTT GCCTCATCGT
    AGGGAAGGAA TAACAGACGA TCTTCCACCA CCACCTGACC CACCGCCTGG TCAGGGTTTA
    AGGCAGCAAA TAGGCTCGGG CCAGCGCGGA GGCTCGGCAG AGAGGAAAGG AAGCTCTCTT
    GAGAGGCAGC ATGCACCCAG CGTAGATGAC ACAAAGAGCT CCTTGGACCG GCAAGCTAGG
    ACGTCACTAG AGTGGCAGCG GCAAACCCAA GAGTGGATAA GCTCTGCAGA ACGCCAAGAG
    GATTTCAGGA AAGCCCAACA CAAGCAAGCA TTCGGATCAG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154026.1
    CDS825..5567
    Translation

    Target ORF information:

    RefSeq Version XM_015298540.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGTCTGC TGACGCCACT CCTGCTCTTT GGATTTGCAT TTTTTCAAGT CTACGGATCC 
    CGTCTCCGCC AGGAGGACTT TCCACCGCGG ATTGTTGAGC ATCCTTCAGA TGTGATAGTC
    TCTAAAGGAG AACCAACAAC CCTGAACTGT AAAGCTGAAG GACGGCCAAC TCCTACTATT
    GAATGGTACA AAGATGGAGA GCGAGTGGAA ACTGACAAAG ATGATCCTCG CTCCCACCGT
    ATGCTGCTGC CTAGCGGATC TTTATTTTTC TTACGTATTG TGCATGGACG CAGGAGTAAA
    CCTGACGAAG GAAGTTATGT TTGCGTAGCA AGGAACTATC TTGGAGAAGC TGTGAGTCGC
    AATGCATCTC TGGAAGTGGC ATTGCTACGA GATGACTTCC GCCAAAACCC TACAGATGTT
    GTAGTGGCAG CTGGAGAACC TGCAATACTG GAGTGTCAGC CACCTCGTGG ACACCCTGAG
    CCTACCATCT ACTGGAAAAA AGATAAAGTC CGTATTGATG ACAGGGAAGA GCGGATCAGT
    ATACGTGGTG GGAAGCTAAT GATCTCCAAC ACACGAAAAA GTGATGCTGG CATGTACACT
    TGCGTTGGAA CCAACATGGT GGGGGAACGA GACAGTGATC CAGCAGAGCT GACTGTCTTT
    GAGCGGCCCA CGTTTCTCAG GAGACCAATC AACCAGGTGG TGCTGGAGGA GGAGGCCGTG
    GATTTCCGGT GCCAGGTGCA GGGGGACCCG CAGCCGACCG TCCGCTGGAA GAAGGATGAC
    GCAGACCTGC CAAGAGGAAG GTATGACATT AAAGATGACT ACACTTTGCG CATCAAGAAG
    GCTATGAGCA CAGATGAAGG CACCTACACG TGCATTGCTG AGAATCGAGT TGGAAAAGTG
    GAAGCATCTG CTACCCTTAC TGTGCGAGCT CGCCCCGTTG CTCCCCCACA GTTTGTGGTG
    AGACCACGAG ATCAGATTGT AGCCCAGGGT CGAACTGTGA CTTTTCCTTG TGAAACTAAA
    GGAAATCCCC AGCCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATCT GCTCTTCCCA
    AACCAGCCTC TCCAACCCAA CAGTAGATAC TCAGTATCAC CAACTGGAGA CCTCACTATT
    ACCAACATTC AACGGTCTGA TGCAGGCTAC TACATTTGTC AAGCACTGAC GGTTGCTGGA
    AGCATTTTAG CAAAGGCTCA GCTGGAGGTT ACTGATGTCT TGACAGATAG ACCTCCACCC
    ATCATCCTCC AGGGGCCTGT CAATCAGACG CTGGCAGTGG ACGGAACAGC TCTGCTGAAG
    TGCAAGGCTA CTGGTGACCC ACTTCCTGTT ATCAGCTGGT TAAAAGAAGG CTTCACTTTT
    CTGGGCAGAG ACCCTCGAAC ATCCATACAG GATCAGGGGA CCCTTCAGAT TAAAACTCTG
    CGGCTGTCTG ATACTGGAAC TTACACTTGT GTTGCAACAA GTTCAAGTGG GGAGACATCA
    TGGAGTGCTG TTCTGGATGT GACAGAGTTG GGTGGAGCAA CAGTCAGTAA AAATTATGAC
    ATAAATGACC TCCCTGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCCTGT CCTGGCAACC AGGGACCCCT GGAAGCCTAC CAGCTAGTGC ATATATCATT
    GAGGCTTTTA GTCAATCTGT GAGCAATAGT TGGCAAACAG TGGCTAACCA TGTGAAGACC
    ACTCTCTACA CTGTGAGAGG ACTGCGTCCC AATACCATCT ATCTGTTTAT GGTGAGAGCT
    ATCAATTCAC AAGGTCTGAG TGATCCCAGC CCTATGTCAG ATCCTGTTCG AACACAAGAT
    ATCAGTCCAC CAGCACAAGG TGTGGATCAC AGGCAAGTCC AGAAGGAACT GGGAGACGTC
    ATTGTACGAC TGCATAACCC TGTTGTGCTG ACACCCACAA CAGTTCAAGT TACCTGGACA
    GTTGACCGTC AATCGCAGTT TATTCAGGGC TACCGAGTAA TGTATCGCCA AACGTCTGGC
    CTACCGTCTC CAGCTGTATG GCAGAATTTG GAGGTCAAAG TCCCAACAGA GCGCAGTGCT
    GTTCTAGTCA ACTTGAAAAA AGGAGTCACT TATGAAATTA AAGTTCGCCC GTACTTCAAT
    GAATTTCAAG GAATGGACAG TGAATCAAAG TCTGCTCGTA CCACAGAGGA AGCTCCAAGT
    GCCCCTCCTC AGTCTGTTAC TGTCCTGACA GTTGGAAATC ACAACAGCAC AAGCATCAGC
    ATCTCCTGGG ATCCTCCCCC TCCAGATCAC CAAAATGGAA TCATCCAGGA GTACAAGATC
    TGGTGTCTAG GTAATGAGAC TCGATTTCAT ATCAACAAAA CAGTAGATGC AGCCATTCGG
    TCTGTAGTAA TAGGTGGCCT GTATCCAGGT ATTCAGTACC GTGTAGAAGT TGCTGCCAGC
    ACCAGTGCAG GTGTGGGAGT GAAAAGTGAG CCACAACCCA TAATAATTGG AGGCCGTAAC
    GAAGTTGTCA TCACTGAAAA TAACAACAGC ATAACTGAGC AAATCACTGA TGTTGTCAAA
    CAGCCTGCCT TTATAGCTGG CATCGGTGGT GCATGTTGGG TAATTCTGAT GGGTTTCAGC
    ATCTGGCTGT ACTGGCGCAG AAAGAAGAGG AAGGGGCTCA GTAATTATGC TGTTCAGTCC
    TTCACCTTCA CCCCTGCAGT CACATTCCAA AGAGGAGATG GAGGACTAAT GAGCAATGGA
    AGTCGACCAG GTTTGTTGAA TGCAAGTGAC CCCAGTTATC CTTGGCTTGC AGACTCTTGG
    CCTGCCACAA GTCTGCCAGT TAACAACAGC TCTAGTGGAC CCAATGACCT TGCCAATTTC
    AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA GGACAAGGAG ATAAAACTGC TACTATGCTT
    TCTGATGGAG CCATTTACAG CAGCATTGAC TTCACGACAA AAACTAGTTA CAACAGTTCC
    AGCCAAATAA CGCAAGCTAC ACCATATGCC ACCACCCAGA TACTGCATTC CAACAGCATC
    CATGAGTTGG CAGTCGACTT ACCTGATCCT CAGTGGAAAA GCTCAATTCA GCAAAAAAAT
    GACCTGATGG GATTTGGCTA CTCTCTCCCA GACCAAGGCA AAGGCAACAA TGCCTTGCTT
    TACATCCCTG ACTACCGCGT GGCTGAGGGA TTGGCTAATA GATTGCCACA CAACCAGTCA
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCCGAAA GGAGTGGCAG CCTCTCAGGT
    GGCAAAGGAG GGAAAAAGAA GAAAAGCAAA AATACTGCCA AGCCTCAGAA AAGTAATGGT
    TCAAACTGGG CCAGTGTTCC CCTCCCACCC CCTCCTGTGC AGCCGCTTCC AGGCACAGAG
    CTGGAACACT ACGGGGTGGA TCAACAAGAA GCAGGATATG ACAGTGATGG TTGGTGTCCA
    CCCTTGCCTG TTCAGACCTA CCTCCATCAG GGCATGGAGG ATGAGCTTGA AGAAGATGAT
    GATCGTGTTC CCACACCTCC TGTGCGAGGA GTAGCTTCAT CACCTGCGAT CTCCTTTGGG
    CAACAATCAA CTGCAACCCT CACGCCTTCT CCGCGAGAGG AGATGCAGCC AATGCTTCAA
    GCCCACCTCG ATGAATTAAC CAGGGCCTAC CAGTTTGATA TAGCAAAGCA GACATGGCAC
    ATCCAAAGCA ATACACAACC TCCTCAGGCT CCAGTTCCAC CCCTAGGATA TGTATCTGGA
    AACCTTATTT CAGACTTGGA AACAGACGTT CCAGATGATG ATGAGGATGA TATTGAAGAA
    GAAACTCTAG AAATCAATAG GCCACTGAGA GGTCTAGAGC ATACTCCAGG CTCCAGTATG
    GACAATCTAG ACAGCTCTGT GACAGGTTCA ATGGTAAATG GATGGGGTTC TGCGTCTGAT
    GAGGACCGTA ACTTTTCTAG TCATAGATCT AGTGTAGGTA GCTCCTCAGA TGACTCGATC
    TTTACCAGCG GCAGTTTTGC ACAAGCACTG GTTGCAGCAG CAGATAAAGC TGGTTTTAGG
    CTGGATGGAA CCAGCCTGAC AAGAACAGGC AAAGCATATC CTTCCTCTCA GAGACCTCGG
    CCAAGCAGTC CTTTTTCTAC TGACAGCAAC ACCAGTGCAG CTGTCACTCA GAGTCAGAGG
    CCAAGGCCCA CTAAAAAACA CAAGGGAGGC CGGTTGGACC AACCCCCCTT GCCTCATCGT
    AGGGAAGGAA TAACAGACGA TCTTCCACCA CCACCTGACC CACCGCCTGG TCAGGGTTTA
    AGGCAGCAAA TAGGCTCGGG CCAGCGCGGA GGCTCGGCAG AGAGGAAAGG AAGCTCTCTT
    GAGAGGCAGC ATGCACCCAG CGTAGATGAC ACAAAGAGCT CCTTGGACCG GCAAGCTAGG
    ACGTCACTAG AGTGGCAGCG GCAAACCCAA GAGTGGATAA GCTCTGCAGA ACGCCAAGAG
    GATTTCAGGA AAGCCCAACA CAAGCAAGCA TTCGGATCAG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30211
    Clone ID Related Accession (Same CDS sequence) XM_015298540.1 , XM_015298540.2
    Accession Version XM_015298540.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGTCTGC TGACGCCACT CCTGCTCTTT GGATTTGCAT TTTTTCAAGT CTACGGATCC 
    CGTCTCCGCC AGGAGGACTT TCCACCGCGG ATTGTTGAGC ATCCTTCAGA TGTGATAGTC
    TCTAAAGGAG AACCAACAAC CCTGAACTGT AAAGCTGAAG GACGGCCAAC TCCTACTATT
    GAATGGTACA AAGATGGAGA GCGAGTGGAA ACTGACAAAG ATGATCCTCG CTCCCACCGT
    ATGCTGCTGC CTAGCGGATC TTTATTTTTC TTACGTATTG TGCATGGACG CAGGAGTAAA
    CCTGACGAAG GAAGTTATGT TTGCGTAGCA AGGAACTATC TTGGAGAAGC TGTGAGTCGC
    AATGCATCTC TGGAAGTGGC ATTGCTACGA GATGACTTCC GCCAAAACCC TACAGATGTT
    GTAGTGGCAG CTGGAGAACC TGCAATACTG GAGTGTCAGC CACCTCGTGG ACACCCTGAG
    CCTACCATCT ACTGGAAAAA AGATAAAGTC CGTATTGATG ACAGGGAAGA GCGGATCAGT
    ATACGTGGTG GGAAGCTAAT GATCTCCAAC ACACGAAAAA GTGATGCTGG CATGTACACT
    TGCGTTGGAA CCAACATGGT GGGGGAACGA GACAGTGATC CAGCAGAGCT GACTGTCTTT
    GAGCGGCCCA CGTTTCTCAG GAGACCAATC AACCAGGTGG TGCTGGAGGA GGAGGCCGTG
    GATTTCCGGT GCCAGGTGCA GGGGGACCCG CAGCCGACCG TCCGCTGGAA GAAGGATGAC
    GCAGACCTGC CAAGAGGAAG GTATGACATT AAAGATGACT ACACTTTGCG CATCAAGAAG
    GCTATGAGCA CAGATGAAGG CACCTACACG TGCATTGCTG AGAATCGAGT TGGAAAAGTG
    GAAGCATCTG CTACCCTTAC TGTGCGAGCT CGCCCCGTTG CTCCCCCACA GTTTGTGGTG
    AGACCACGAG ATCAGATTGT AGCCCAGGGT CGAACTGTGA CTTTTCCTTG TGAAACTAAA
    GGAAATCCCC AGCCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATCT GCTCTTCCCA
    AACCAGCCTC TCCAACCCAA CAGTAGATAC TCAGTATCAC CAACTGGAGA CCTCACTATT
    ACCAACATTC AACGGTCTGA TGCAGGCTAC TACATTTGTC AAGCACTGAC GGTTGCTGGA
    AGCATTTTAG CAAAGGCTCA GCTGGAGGTT ACTGATGTCT TGACAGATAG ACCTCCACCC
    ATCATCCTCC AGGGGCCTGT CAATCAGACG CTGGCAGTGG ACGGAACAGC TCTGCTGAAG
    TGCAAGGCTA CTGGTGACCC ACTTCCTGTT ATCAGCTGGT TAAAAGAAGG CTTCACTTTT
    CTGGGCAGAG ACCCTCGAAC ATCCATACAG GATCAGGGGA CCCTTCAGAT TAAAACTCTG
    CGGCTGTCTG ATACTGGAAC TTACACTTGT GTTGCAACAA GTTCAAGTGG GGAGACATCA
    TGGAGTGCTG TTCTGGATGT GACAGAGTTG GGTGGAGCAA CAGTCAGTAA AAATTATGAC
    ATAAATGACC TCCCTGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCCTGT CCTGGCAACC AGGGACCCCT GGAAGCCTAC CAGCTAGTGC ATATATCATT
    GAGGCTTTTA GTCAATCTGT GAGCAATAGT TGGCAAACAG TGGCTAACCA TGTGAAGACC
    ACTCTCTACA CTGTGAGAGG ACTGCGTCCC AATACCATCT ATCTGTTTAT GGTGAGAGCT
    ATCAATTCAC AAGGTCTGAG TGATCCCAGC CCTATGTCAG ATCCTGTTCG AACACAAGAT
    ATCAGTCCAC CAGCACAAGG TGTGGATCAC AGGCAAGTCC AGAAGGAACT GGGAGACGTC
    ATTGTACGAC TGCATAACCC TGTTGTGCTG ACACCCACAA CAGTTCAAGT TACCTGGACA
    GTTGACCGTC AATCGCAGTT TATTCAGGGC TACCGAGTAA TGTATCGCCA AACGTCTGGC
    CTACCGTCTC CAGCTGTATG GCAGAATTTG GAGGTCAAAG TCCCAACAGA GCGCAGTGCT
    GTTCTAGTCA ACTTGAAAAA AGGAGTCACT TATGAAATTA AAGTTCGCCC GTACTTCAAT
    GAATTTCAAG GAATGGACAG TGAATCAAAG TCTGCTCGTA CCACAGAGGA AGCTCCAAGT
    GCCCCTCCTC AGTCTGTTAC TGTCCTGACA GTTGGAAATC ACAACAGCAC AAGCATCAGC
    ATCTCCTGGG ATCCTCCCCC TCCAGATCAC CAAAATGGAA TCATCCAGGA GTACAAGATC
    TGGTGTCTAG GTAATGAGAC TCGATTTCAT ATCAACAAAA CAGTAGATGC AGCCATTCGG
    TCTGTAGTAA TAGGTGGCCT GTATCCAGGT ATTCAGTACC GTGTAGAAGT TGCTGCCAGC
    ACCAGTGCAG GTGTGGGAGT GAAAAGTGAG CCACAACCCA TAATAATTGG AGGCCGTAAC
    GAAGTTGTCA TCACTGAAAA TAACAACAGC ATAACTGAGC AAATCACTGA TGTTGTCAAA
    CAGCCTGCCT TTATAGCTGG CATCGGTGGT GCATGTTGGG TAATTCTGAT GGGTTTCAGC
    ATCTGGCTGT ACTGGCGCAG AAAGAAGAGG AAGGGGCTCA GTAATTATGC TGTTCAGTCC
    TTCACCTTCA CCCCTGCAGT CACATTCCAA AGAGGAGATG GAGGACTAAT GAGCAATGGA
    AGTCGACCAG GTTTGTTGAA TGCAAGTGAC CCCAGTTATC CTTGGCTTGC AGACTCTTGG
    CCTGCCACAA GTCTGCCAGT TAACAACAGC TCTAGTGGAC CCAATGACCT TGCCAATTTC
    AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA GGACAAGGAG ATAAAACTGC TACTATGCTT
    TCTGATGGAG CCATTTACAG CAGCATTGAC TTCACGACAA AAACTAGTTA CAACAGTTCC
    AGCCAAATAA CGCAAGCTAC ACCATATGCC ACCACCCAGA TACTGCATTC CAACAGCATC
    CATGAGTTGG CAGTCGACTT ACCTGATCCT CAGTGGAAAA GCTCAATTCA GCAAAAAAAT
    GACCTGATGG GATTTGGCTA CTCTCTCCCA GACCAAGGCA AAGGCAACAA TGCCTTGCTT
    TACATCCCTG ACTACCGCGT GGCTGAGGGA TTGGCTAATA GATTGCCACA CAACCAGTCA
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCCGAAA GGAGTGGCAG CCTCTCAGGT
    GGCAAAGGAG GGAAAAAGAA GAAAAGCAAA AATACTGCCA AGCCTCAGAA AAGTAATGGT
    TCAAACTGGG CCAGTGTTCC CCTCCCACCC CCTCCTGTGC AGCCGCTTCC AGGCACAGAG
    CTGGAACACT ACGGGGTGGA TCAACAAGAA GCAGGATATG ACAGTGATGG TTGGTGTCCA
    CCCTTGCCTG TTCAGACCTA CCTCCATCAG GGCATGGAGG ATGAGCTTGA AGAAGATGAT
    GATCGTGTTC CCACACCTCC TGTGCGAGGA GTAGCTTCAT CACCTGCGAT CTCCTTTGGG
    CAACAATCAA CTGCAACCCT CACGCCTTCT CCGCGAGAGG AGATGCAGCC AATGCTTCAA
    GCCCACCTCG ATGAATTAAC CAGGGCCTAC CAGTTTGATA TAGCAAAGCA GACATGGCAC
    ATCCAAAGCA ATACACAACC TCCTCAGGCT CCAGTTCCAC CCCTAGGATA TGTATCTGGA
    AACCTTATTT CAGACTTGGA AACAGACGTT CCAGATGATG ATGAGGATGA TATTGAAGAA
    GAAACTCTAG AAATCAATAG GCCACTGAGA GGTCTAGAGC ATACTCCAGG CTCCAGTATG
    GACAATCTAG ACAGCTCTGT GACAGGTTCA ATGGTAAATG GATGGGGTTC TGCGTCTGAT
    GAGGACCGTA ACTTTTCTAG TCATAGATCT AGTGTAGGTA GCTCCTCAGA TGACTCGATC
    TTTACCAGCG GCAGTTTTGC ACAAGCACTG GTTGCAGCAG CAGATAAAGC TGGTTTTAGG
    CTGGATGGAA CCAGCCTGAC AAGAACAGGC AAAGCATATC CTTCCTCTCA GAGACCTCGG
    CCAAGCAGTC CTTTTTCTAC TGACAGCAAC ACCAGTGCAG CTGTCACTCA GAGTCAGAGG
    CCAAGGCCCA CTAAAAAACA CAAGGGAGGC CGGTTGGACC AACCCCCCTT GCCTCATCGT
    AGGGAAGGAA TAACAGACGA TCTTCCACCA CCACCTGACC CACCGCCTGG TCAGGGTTTA
    AGGCAGCAAA TAGGCTCGGG CCAGCGCGGA GGCTCGGCAG AGAGGAAAGG AAGCTCTCTT
    GAGAGGCAGC ATGCACCCAG CGTAGATGAC ACAAAGAGCT CCTTGGACCG GCAAGCTAGG
    ACGTCACTAG AGTGGCAGCG GCAAACCCAA GAGTGGATAA GCTCTGCAGA ACGCCAAGAG
    GATTTCAGGA AAGCCCAACA CAAGCAAGCA TTCGGATCAG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154026.1
    CDS835..5577
    Translation

    Target ORF information:

    RefSeq Version XM_015298540.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGTCTGC TGACGCCACT CCTGCTCTTT GGATTTGCAT TTTTTCAAGT CTACGGATCC 
    CGTCTCCGCC AGGAGGACTT TCCACCGCGG ATTGTTGAGC ATCCTTCAGA TGTGATAGTC
    TCTAAAGGAG AACCAACAAC CCTGAACTGT AAAGCTGAAG GACGGCCAAC TCCTACTATT
    GAATGGTACA AAGATGGAGA GCGAGTGGAA ACTGACAAAG ATGATCCTCG CTCCCACCGT
    ATGCTGCTGC CTAGCGGATC TTTATTTTTC TTACGTATTG TGCATGGACG CAGGAGTAAA
    CCTGACGAAG GAAGTTATGT TTGCGTAGCA AGGAACTATC TTGGAGAAGC TGTGAGTCGC
    AATGCATCTC TGGAAGTGGC ATTGCTACGA GATGACTTCC GCCAAAACCC TACAGATGTT
    GTAGTGGCAG CTGGAGAACC TGCAATACTG GAGTGTCAGC CACCTCGTGG ACACCCTGAG
    CCTACCATCT ACTGGAAAAA AGATAAAGTC CGTATTGATG ACAGGGAAGA GCGGATCAGT
    ATACGTGGTG GGAAGCTAAT GATCTCCAAC ACACGAAAAA GTGATGCTGG CATGTACACT
    TGCGTTGGAA CCAACATGGT GGGGGAACGA GACAGTGATC CAGCAGAGCT GACTGTCTTT
    GAGCGGCCCA CGTTTCTCAG GAGACCAATC AACCAGGTGG TGCTGGAGGA GGAGGCCGTG
    GATTTCCGGT GCCAGGTGCA GGGGGACCCG CAGCCGACCG TCCGCTGGAA GAAGGATGAC
    GCAGACCTGC CAAGAGGAAG GTATGACATT AAAGATGACT ACACTTTGCG CATCAAGAAG
    GCTATGAGCA CAGATGAAGG CACCTACACG TGCATTGCTG AGAATCGAGT TGGAAAAGTG
    GAAGCATCTG CTACCCTTAC TGTGCGAGCT CGCCCCGTTG CTCCCCCACA GTTTGTGGTG
    AGACCACGAG ATCAGATTGT AGCCCAGGGT CGAACTGTGA CTTTTCCTTG TGAAACTAAA
    GGAAATCCCC AGCCAGCTGT TTTTTGGCAA AAAGAAGGAA GCCAGAATCT GCTCTTCCCA
    AACCAGCCTC TCCAACCCAA CAGTAGATAC TCAGTATCAC CAACTGGAGA CCTCACTATT
    ACCAACATTC AACGGTCTGA TGCAGGCTAC TACATTTGTC AAGCACTGAC GGTTGCTGGA
    AGCATTTTAG CAAAGGCTCA GCTGGAGGTT ACTGATGTCT TGACAGATAG ACCTCCACCC
    ATCATCCTCC AGGGGCCTGT CAATCAGACG CTGGCAGTGG ACGGAACAGC TCTGCTGAAG
    TGCAAGGCTA CTGGTGACCC ACTTCCTGTT ATCAGCTGGT TAAAAGAAGG CTTCACTTTT
    CTGGGCAGAG ACCCTCGAAC ATCCATACAG GATCAGGGGA CCCTTCAGAT TAAAACTCTG
    CGGCTGTCTG ATACTGGAAC TTACACTTGT GTTGCAACAA GTTCAAGTGG GGAGACATCA
    TGGAGTGCTG TTCTGGATGT GACAGAGTTG GGTGGAGCAA CAGTCAGTAA AAATTATGAC
    ATAAATGACC TCCCTGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCCTGT CCTGGCAACC AGGGACCCCT GGAAGCCTAC CAGCTAGTGC ATATATCATT
    GAGGCTTTTA GTCAATCTGT GAGCAATAGT TGGCAAACAG TGGCTAACCA TGTGAAGACC
    ACTCTCTACA CTGTGAGAGG ACTGCGTCCC AATACCATCT ATCTGTTTAT GGTGAGAGCT
    ATCAATTCAC AAGGTCTGAG TGATCCCAGC CCTATGTCAG ATCCTGTTCG AACACAAGAT
    ATCAGTCCAC CAGCACAAGG TGTGGATCAC AGGCAAGTCC AGAAGGAACT GGGAGACGTC
    ATTGTACGAC TGCATAACCC TGTTGTGCTG ACACCCACAA CAGTTCAAGT TACCTGGACA
    GTTGACCGTC AATCGCAGTT TATTCAGGGC TACCGAGTAA TGTATCGCCA AACGTCTGGC
    CTACCGTCTC CAGCTGTATG GCAGAATTTG GAGGTCAAAG TCCCAACAGA GCGCAGTGCT
    GTTCTAGTCA ACTTGAAAAA AGGAGTCACT TATGAAATTA AAGTTCGCCC GTACTTCAAT
    GAATTTCAAG GAATGGACAG TGAATCAAAG TCTGCTCGTA CCACAGAGGA AGCTCCAAGT
    GCCCCTCCTC AGTCTGTTAC TGTCCTGACA GTTGGAAATC ACAACAGCAC AAGCATCAGC
    ATCTCCTGGG ATCCTCCCCC TCCAGATCAC CAAAATGGAA TCATCCAGGA GTACAAGATC
    TGGTGTCTAG GTAATGAGAC TCGATTTCAT ATCAACAAAA CAGTAGATGC AGCCATTCGG
    TCTGTAGTAA TAGGTGGCCT GTATCCAGGT ATTCAGTACC GTGTAGAAGT TGCTGCCAGC
    ACCAGTGCAG GTGTGGGAGT GAAAAGTGAG CCACAACCCA TAATAATTGG AGGCCGTAAC
    GAAGTTGTCA TCACTGAAAA TAACAACAGC ATAACTGAGC AAATCACTGA TGTTGTCAAA
    CAGCCTGCCT TTATAGCTGG CATCGGTGGT GCATGTTGGG TAATTCTGAT GGGTTTCAGC
    ATCTGGCTGT ACTGGCGCAG AAAGAAGAGG AAGGGGCTCA GTAATTATGC TGTTCAGTCC
    TTCACCTTCA CCCCTGCAGT CACATTCCAA AGAGGAGATG GAGGACTAAT GAGCAATGGA
    AGTCGACCAG GTTTGTTGAA TGCAAGTGAC CCCAGTTATC CTTGGCTTGC AGACTCTTGG
    CCTGCCACAA GTCTGCCAGT TAACAACAGC TCTAGTGGAC CCAATGACCT TGCCAATTTC
    AGTCGTGGAG ATGTGTTGCC ACCAGTGCCA GGACAAGGAG ATAAAACTGC TACTATGCTT
    TCTGATGGAG CCATTTACAG CAGCATTGAC TTCACGACAA AAACTAGTTA CAACAGTTCC
    AGCCAAATAA CGCAAGCTAC ACCATATGCC ACCACCCAGA TACTGCATTC CAACAGCATC
    CATGAGTTGG CAGTCGACTT ACCTGATCCT CAGTGGAAAA GCTCAATTCA GCAAAAAAAT
    GACCTGATGG GATTTGGCTA CTCTCTCCCA GACCAAGGCA AAGGCAACAA TGCCTTGCTT
    TACATCCCTG ACTACCGCGT GGCTGAGGGA TTGGCTAATA GATTGCCACA CAACCAGTCA
    CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCCGAAA GGAGTGGCAG CCTCTCAGGT
    GGCAAAGGAG GGAAAAAGAA GAAAAGCAAA AATACTGCCA AGCCTCAGAA AAGTAATGGT
    TCAAACTGGG CCAGTGTTCC CCTCCCACCC CCTCCTGTGC AGCCGCTTCC AGGCACAGAG
    CTGGAACACT ACGGGGTGGA TCAACAAGAA GCAGGATATG ACAGTGATGG TTGGTGTCCA
    CCCTTGCCTG TTCAGACCTA CCTCCATCAG GGCATGGAGG ATGAGCTTGA AGAAGATGAT
    GATCGTGTTC CCACACCTCC TGTGCGAGGA GTAGCTTCAT CACCTGCGAT CTCCTTTGGG
    CAACAATCAA CTGCAACCCT CACGCCTTCT CCGCGAGAGG AGATGCAGCC AATGCTTCAA
    GCCCACCTCG ATGAATTAAC CAGGGCCTAC CAGTTTGATA TAGCAAAGCA GACATGGCAC
    ATCCAAAGCA ATACACAACC TCCTCAGGCT CCAGTTCCAC CCCTAGGATA TGTATCTGGA
    AACCTTATTT CAGACTTGGA AACAGACGTT CCAGATGATG ATGAGGATGA TATTGAAGAA
    GAAACTCTAG AAATCAATAG GCCACTGAGA GGTCTAGAGC ATACTCCAGG CTCCAGTATG
    GACAATCTAG ACAGCTCTGT GACAGGTTCA ATGGTAAATG GATGGGGTTC TGCGTCTGAT
    GAGGACCGTA ACTTTTCTAG TCATAGATCT AGTGTAGGTA GCTCCTCAGA TGACTCGATC
    TTTACCAGCG GCAGTTTTGC ACAAGCACTG GTTGCAGCAG CAGATAAAGC TGGTTTTAGG
    CTGGATGGAA CCAGCCTGAC AAGAACAGGC AAAGCATATC CTTCCTCTCA GAGACCTCGG
    CCAAGCAGTC CTTTTTCTAC TGACAGCAAC ACCAGTGCAG CTGTCACTCA GAGTCAGAGG
    CCAAGGCCCA CTAAAAAACA CAAGGGAGGC CGGTTGGACC AACCCCCCTT GCCTCATCGT
    AGGGAAGGAA TAACAGACGA TCTTCCACCA CCACCTGACC CACCGCCTGG TCAGGGTTTA
    AGGCAGCAAA TAGGCTCGGG CCAGCGCGGA GGCTCGGCAG AGAGGAAAGG AAGCTCTCTT
    GAGAGGCAGC ATGCACCCAG CGTAGATGAC ACAAAGAGCT CCTTGGACCG GCAAGCTAGG
    ACGTCACTAG AGTGGCAGCG GCAAACCCAA GAGTGGATAA GCTCTGCAGA ACGCCAAGAG
    GATTTCAGGA AAGCCCAACA CAAGCAAGCA TTCGGATCAG AAGAGGCACT GGTACCATAC
    AGTAAACCCA ACTTCCCATC CCCTGGTGGC CACAGCTCTT CAGGAACAGC TTCTTCTAAA
    GGATCGACTG GACCTAGAAA AGGTGAAGTC TTTCGAGGAG GTCACCAACG CAATGCCAGT
    GACCTTCTTG ATGTAGGCTA TTTGGGATCA AACAGTCAAG GACAGTTTAC TGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30212
    Clone ID Related Accession (Same CDS sequence) XM_015298548.1 , XM_015298548.2
    Accession Version XM_015298548.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154034.1
    CDS408..5123
    Translation

    Target ORF information:

    RefSeq Version XM_015298548.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30212
    Clone ID Related Accession (Same CDS sequence) XM_015298548.1 , XM_015298548.2
    Accession Version XM_015298548.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154034.1
    CDS377..5092
    Translation

    Target ORF information:

    RefSeq Version XM_015298548.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298548.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTATCAT CAGTGAGACG GGGGAAAATC ACTATGCTTG AACTTCTTTT CATTTTGATG 
    TTGCTCTTCT CTGGACCAAC CCTGAGCAAG CTTAGCAGGA CCAAAGGCAA ACACTGGAAG
    GGGGGATCCC GTCTCCGCCA GGAGGACTTT CCACCGCGGA TTGTTGAGCA TCCTTCAGAT
    GTGATAGTCT CTAAAGGAGA ACCAACAACC CTGAACTGTA AAGCTGAAGG ACGGCCAACT
    CCTACTATTG AATGGTACAA AGATGGAGAG CGAGTGGAAA CTGACAAAGA TGATCCTCGC
    TCCCACCGTA TGCTGCTGCC TAGCGGATCT TTATTTTTCT TACGTATTGT GCATGGACGC
    AGGAGTAAAC CTGACGAAGG AAGTTATGTT TGCGTAGCAA GGAACTATCT TGGAGAAGCT
    GTGAGTCGCA ATGCATCTCT GGAAGTGGCA TTGCTACGAG ATGACTTCCG CCAAAACCCT
    ACAGATGTTG TAGTGGCAGC TGGAGAACCT GCAATACTGG AGTGTCAGCC ACCTCGTGGA
    CACCCTGAGC CTACCATCTA CTGGAAAAAA GATAAAGTCC GTATTGATGA CAGGGAAGAG
    CGGATCAGTA TACGTGGTGG GAAGCTAATG ATCTCCAACA CACGAAAAAG TGATGCTGGC
    ATGTACACTT GCGTTGGAAC CAACATGGTG GGGGAACGAG ACAGTGATCC AGCAGAGCTG
    ACTGTCTTTG AGCGGCCCAC GTTTCTCAGG AGACCAATCA ACCAGGTGGT GCTGGAGGAG
    GAGGCCGTGG ATTTCCGGTG CCAGGTGCAG GGGGACCCGC AGCCGACCGT CCGCTGGAAG
    AAGGATGACG CAGACCTGCC AAGAGGAAGG TATGACATTA AAGATGACTA CACTTTGCGC
    ATCAAGAAGG CTATGAGCAC AGATGAAGGC ACCTACACGT GCATTGCTGA GAATCGAGTT
    GGAAAAGTGG AAGCATCTGC TACCCTTACT GTGCGAGCTC GCCCCGTTGC TCCCCCACAG
    TTTGTGGTGA GACCACGAGA TCAGATTGTA GCCCAGGGTC GAACTGTGAC TTTTCCTTGT
    GAAACTAAAG GAAATCCCCA GCCAGCTGTT TTTTGGCAAA AAGAAGGAAG CCAGAATCTG
    CTCTTCCCAA ACCAGCCTCT CCAACCCAAC AGTAGATACT CAGTATCACC AACTGGAGAC
    CTCACTATTA CCAACATTCA ACGGTCTGAT GCAGGCTACT ACATTTGTCA AGCACTGACG
    GTTGCTGGAA GCATTTTAGC AAAGGCTCAG CTGGAGGTTA CTGATGTCTT GACAGATAGA
    CCTCCACCCA TCATCCTCCA GGGGCCTGTC AATCAGACGC TGGCAGTGGA CGGAACAGCT
    CTGCTGAAGT GCAAGGCTAC TGGTGACCCA CTTCCTGTTA TCAGCTGGTT AAAAGAAGGC
    TTCACTTTTC TGGGCAGAGA CCCTCGAACA TCCATACAGG ATCAGGGGAC CCTTCAGATT
    AAAACTCTGC GGCTGTCTGA TACTGGAACT TACACTTGTG TTGCAACAAG TTCAAGTGGG
    GAGACATCAT GGAGTGCTGT TCTGGATGTG ACAGAGTTGG GTGGAGCAAC AGTCAGTAAA
    AATTATGACA TAAATGACCT CCCTGGACCA CCATCCAAAC CTCAGGTCAC TGATGTTACT
    AAGAACAGTG TCACCCTGTC CTGGCAACCA GGGACCCCTG GAAGCCTACC AGCTAGTGCA
    TATATCATTG AGGCTTTTAG TCAATCTGTG AGCAATAGTT GGCAAACAGT GGCTAACCAT
    GTGAAGACCA CTCTCTACAC TGTGAGAGGA CTGCGTCCCA ATACCATCTA TCTGTTTATG
    GTGAGAGCTA TCAATTCACA AGGTCTGAGT GATCCCAGCC CTATGTCAGA TCCTGTTCGA
    ACACAAGATA TCAGTCCACC AGCACAAGGT GTGGATCACA GGCAAGTCCA GAAGGAACTG
    GGAGACGTCA TTGTACGACT GCATAACCCT GTTGTGCTGA CACCCACAAC AGTTCAAGTT
    ACCTGGACAG TTGACCGTCA ATCGCAGTTT ATTCAGGGCT ACCGAGTAAT GTATCGCCAA
    ACGTCTGGCC TACCGTCTCC AGCTGTATGG CAGAATTTGG AGGTCAAAGT CCCAACAGAG
    CGCAGTGCTG TTCTAGTCAA CTTGAAAAAA GGAGTCACTT ATGAAATTAA AGTTCGCCCG
    TACTTCAATG AATTTCAAGG AATGGACAGT GAATCAAAGT CTGCTCGTAC CACAGAGGAA
    GCTCCAAGTG CCCCTCCTCA GTCTGTTACT GTCCTGACAG TTGGAAATCA CAACAGCACA
    AGCATCAGCA TCTCCTGGGA TCCTCCCCCT CCAGATCACC AAAATGGAAT CATCCAGGAG
    TACAAGATCT GGTGTCTAGG TAATGAGACT CGATTTCATA TCAACAAAAC AGTAGATGCA
    GCCATTCGGT CTGTAGTAAT AGGTGGCCTG TATCCAGGTA TTCAGTACCG TGTAGAAGTT
    GCTGCCAGCA CCAGTGCAGG TGTGGGAGTG AAAAGTGAGC CACAACCCAT AATAATTGGA
    GGCCGTAACG AAGTTGTCAT CACTGAAAAT AACAACAGCA TAACTGAGCA AATCACTGAT
    GTTGTCAAAC AGCCTGCCTT TATAGCTGGC ATCGGTGGTG CATGTTGGGT AATTCTGATG
    GGTTTCAGCA TCTGGCTGTA CTGGCGCAGA AAGAAGAGGA AGGGGCTCAG TAATTATGCT
    GTTCAGTCCT TCACCTTCAC CCCTGCAGTC ACATTCCAAA GAGGAGATGG AGGACTAATG
    AGCAATGGAA GTCGACCAGG TTTGTTGAAT GCAAGTGACC CCAGTTATCC TTGGCTTGCA
    GACTCTTGGC CTGCCACAAG TCTGCCAGTT AACAACAGCT CTAGTGGACC CAATGACCTT
    GCCAATTTCA GTCGTGGAGA TGTGTTGCCA CCAGTGCCAG GACAAGGAGA TAAAACTGCT
    ACTATGCTTT CTGATGGAGC CATTTACAGC AGCATTGACT TCACGACAAA AACTAGTTAC
    AACAGTTCCA GCCAAATAAC GCAAGCTACA CCATATGCCA CCACCCAGAT ACTGCATTCC
    AACAGCATCC ATGAGTTGGC AGTCGACTTA CCTGATCCTC AGTGGAAAAG CTCAATTCAG
    CAAAAAAATG ACCTGATGGG ATTTGGCTAC TCTCTCCCAG ACCAAGGCAA AGGCAACAAT
    GCCTTGCTTT ACATCCCTGA CTACCGCGTG GCTGAGGGAT TGGCTAATAG ATTGCCACAC
    AACCAGTCAC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCCGAAAG GAGTGGCAGC
    CTCTCAGGTG GCAAAGGAGG GAAAAAGAAG AAAAGCAAAA ATACTGCCAA GCCTCAGAAA
    AGTAATGGTT CAAACTGGGC CAGTGTTCCC CTCCCACCCC CTCCTGTGCA GCCGCTTCCA
    GGCACAGAGC TGGAACACTA CGGGGTGGAT CAACAAGAAG CAGGATATGA CAGTGATGGT
    TGGTGTCCAC CCTTGCCTGT TCAGACCTAC CTCCATCAGG GCATGGAGGA TGAGCTTGAA
    GAAGATGATG ATCGTGTTCC CACACCTCCT GTGCGAGGAG TAGCTTCATC ACCTGCGATC
    TCCTTTGGGC AACAATCAAC TGCAACCCTC ACGCCTTCTC CGCGAGAGGA GATGCAGCCA
    ATGCTTCAAG CCCACCTCGA TGAATTAACC AGGGCCTACC AGTTTGATAT AGCAAAGCAG
    ACATGGCACA TCCAAAGCAA TACACAACCT CCTCAGGCTC CAGTTCCACC CCTAGGATAT
    GTATCTGGAA ACCTTATTTC AGACTTGGAA ACAGACGTTC CAGATGATGA TGAGGATGAT
    ATTGAAGAAG AAACTCTAGA AATCAATAGG CCACTGAGAG GTCTAGAGCA TACTCCAGGC
    TCCAGTATGG ACAATCTAGA CAGCTCTGTG ACAGGTTCAA TGGTAAATGG ATGGGGTTCT
    GCGTCTGATG AGGACCGTAA CTTTTCTAGT CATAGATCTA GTGTAGGTAG CTCCTCAGAT
    GACTCGATCT TTACCAGCGG CAGTTTTGCA CAAGCACTGG TTGCAGCAGC AGATAAAGCT
    GGTTTTAGGC TGGATGGAAC CAGCCTGACA AGAACAGGCA AAGCATATCC TTCCTCTCAG
    AGACCTCGGC CAAGCAGTCC TTTTTCTACT GACAGCAACA CCAGTGCAGC TGTCACTCAG
    AGTCAGAGGC CAAGGCCCAC TAAAAAACAC AAGGGAGGCC GGTTGGACCA ACCCCCCTTG
    CCTCATCGTA GGGAAGGAAT AACAGACGAT CTTCCACCAC CACCTGACCC ACCGCCTGGT
    CAGGGTTTAA GGCAGCAAAT AGGCTCGGGC CAGCGCGGAG GCTCGGCAGA GAGGAAAGGA
    AGCTCTCTTG AGAGGCAGCA TGCACCCAGC GTAGATGACA CAAAGAGCTC CTTGGACCGG
    CAAGCTAGGA CGTCACTAGA GTGGCAGCGG CAAACCCAAG AGTGGATAAG CTCTGCAGAA
    CGCCAAGAGG ATTTCAGGAA AGCCCAACAC AAGCAAGCAT TCGGATCAGA AGAGGCACTG
    GTACCATACA GTAAACCCAA CTTCCCATCC CCTGGTGGCC ACAGCTCTTC AGGAACAGCT
    TCTTCTAAAG GATCGACTGG ACCTAGAAAA GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30213
    Clone ID Related Accession (Same CDS sequence) XM_015298556.1 , XM_015298556.2
    Accession Version XM_015298556.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Meth