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NKX2-1 cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol NKX2-1
Entrez Gene ID 395322
Full Name NK2 homeobox 1
Synonyms NKX2.1,TITF1
General protein information
Preferred Names

NK2 homeobox 1

Names

homeobox protein Nkx-2.1
homeodomain protein NKx2.1
thyroid transcription factor 1
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015287273.1 XP_015142759.1 homeobox protein Nkx-2.1 isoform X1
XM_015287273.2 XP_015142759.1 homeobox protein Nkx-2.1 isoform X1
XM_015287274.2 XP_015142760.1 homeobox protein Nkx-2.1 isoform X2
NM_204616.1 NP_989947.1 homeobox protein Nkx-2.1
XM_025150545.1 XP_025006313.1 homeobox protein Nkx-2.1 isoform X2
XM_025150547.1 XP_025006315.1 homeobox protein Nkx-2.1 isoform X2
XM_015287274.1 XP_015142760.1 homeobox protein Nkx-2.1 isoform X2
XM_025150546.1 XP_025006314.1 homeobox protein Nkx-2.1 isoform X2

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Nkx2-1 NP_037225.1
Danio rerio (zebrafish) nkx2.1b NP_571851.1
Xenopus tropicalis (tropical clawed frog) nkx2-1 XP_002935383.1
Homo sapiens (human) NKX2-1 NP_001073136.1
Pan troglodytes (chimpanzee) NKX2-1 XP_509911.2
NKX2-1 XP_001089773.1
Bos taurus (cattle) LOC613713 NP_001178387.1
Mus musculus (house mouse) Nkx2-1 NP_001139670.1
Gallus gallus (chicken) NKX2-1 NP_989947.1

Gene Ontology


Bibliography

Related articles in PubMed

Demarcation of ventral territories by the homeobox gene NKX2.1 during early chick development.
Pera EM, Kessel M
Development genes and evolution208(3)168-71(1998 May)

Control of anteroposterior and dorsoventral domains of Nkx-6.1 gene expression relative to other Nkx genes during vertebrate CNS development.
Qiu M, Shimamura K, Sussel L, Chen S, Rubenstein JL
Mechanisms of development72(1-2)77-88(1998 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NKX2-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKX2-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00968 NM_204616.1
Latest version!		 Gallus gallus NK2 homeobox 1 (NKX2-1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
OGa36750 XM_015287273.2
Latest version!		 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OGa36751 XM_025150547.1
Latest version!		 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OGa36751 XM_025150545.1
Latest version!		 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OGa36751 XM_015287274.2
Latest version!		 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OGa36751 XM_025150546.1
Latest version!		 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
View Old Accession Versions >>
OGa36750 XM_015287273.1 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OGa36751 XM_015287274.1 Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa00968
Clone ID Related Accession (Same CDS sequence) NM_204616.1
Accession Version NM_204616.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1035bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-20
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ223618.1. ##Evidence-Data-START## Transcript exon combination :: AJ223618.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CGGACATCTT GAGTCCTTGG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC ACGAGATGCA ACAGGAGAAC GGCTCTTGCC AGCAGCAGCA GTCCCCCAGG 
AGGGTGGCGG TGCCGGTGCT GGTGAAGGAT GGCAAGCCCT GCCAGGCGGG CTCCAACACC 
CCCACCGCAG CCATCCAGAG CCACCCGCAG CAGGCGGCCA CGACCATCAC GGTGGCCACC 
AATGGCAACA GCCTCGGACA GCATCAGAGC CATCAGACAA ACAGTGCGGG GCAGTCTCCC 
GACATGGGAC AGCACTCGGC CAGCCCTTCC TCCCTGCAGA GCCAAGTCTC CAGTTTGTCT 
CACCTGAACT CTTCCACTTC TGACTATGGC ACTGCCATGT CCTGCTCCAC CTTGCTATAC 
GGTAGGACCT GGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_989947.1
CDS22..1056
Translation

Target ORF information:

RefSeq Version NM_204616.1
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_204616.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CGGACATCTT GAGTCCTTGG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC ACGAGATGCA ACAGGAGAAC GGCTCTTGCC AGCAGCAGCA GTCCCCCAGG 
AGGGTGGCGG TGCCGGTGCT GGTGAAGGAT GGCAAGCCCT GCCAGGCGGG CTCCAACACC 
CCCACCGCAG CCATCCAGAG CCACCCGCAG CAGGCGGCCA CGACCATCAC GGTGGCCACC 
AATGGCAACA GCCTCGGACA GCATCAGAGC CATCAGACAA ACAGTGCGGG GCAGTCTCCC 
GACATGGGAC AGCACTCGGC CAGCCCTTCC TCCCTGCAGA GCCAAGTCTC CAGTTTGTCT 
CACCTGAACT CTTCCACTTC TGACTATGGC ACTGCCATGT CCTGCTCCAC CTTGCTATAC 
GGTAGGACCT GGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36750
Clone ID Related Accession (Same CDS sequence) XM_015287273.1 , XM_015287273.2
Accession Version XM_015287273.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1101bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTCCACG CCCTGAGCAG CTGGAGGAGC AACTGGGCGT GCGCTGCGGA GGGCCGCCGA 
ATCATGTCGA TGAGCCCAAA GCATACGACT CCTTTCTCAG TGTCTGACAT CTTGAGTCCT 
TTGGAGGAAA GCTACAAGAA AGTGGGCATG GAGGGCAGTA ACTTGGGGGC TCCCTTGTCA 
GCCTACAGAC AGTCTCAGGT TTCGCAACCG GCCATGCAGC AACACCCCAT GGGCCACAAC 
GGAACGGTGA CTGCCGCCTA CCATATGACA GCGGCAGGGG TCCCCCAGCT CTCCCACGCT 
ACGATGGGGG GCTACTGCAA TGGGAACCTG GGCAACATGA GCGAGCTCCC GCCTTACCAG 
GACACCATGC GGAACAGCGC TTCGGCGACA GGATGGTACG GCACCAACCC GGACCCCCGC 
TTCTCCTCAA TCTCCCGCTT CATGGCGCCG TCCTCGGGCA TGAACATGGG CGGCATGGGC 
GGCCTGAGCT CCCTGGGGGA CGTGAGCAAG AGCATGGCCC CGCTGCAGAG CACGCCGCGG 
AGGAAACGGA GGGTCCTGTT TTCCCAGGCC CAGGTTTACG AGCTGGAGAG ACGTTTCAAG 
CAGCAGAAAT ACCTCTCCGC CCCCGAGAGG GAACATTTAG CCAGCATGAT CCACCTCACC 
CCGACTCAGG TCAAAATCTG GTTCCAGAAC CACCGCTACA AGATGAAGCG GCAGGCCAAA 
GACAAGGCTG CGCAGCAGCA GATGCAACAG GAGAACGGCT CTTGCCAGCA GCAGCAGTCC 
CCCAGGAGGG TGGCGGTGCC GGTGCTGGTG AAGGATGGCA AGCCCTGCCA GGCGGGCTCC 
AACACCCCCA CCGCAGCCAT CCAGAGCCAC CCGCAGCAGG CGGCCACGAC CATCACGGTG 
GCCACCAATG GCAACAGCCT CGGACAGCAT CAGAGCCATC AGACAAACAG TGCGGGGCAG 
TCTCCCGACA TGGGACAGCA CTCGGCCAGC CCTTCCTCCC TGCAGAGCCA AGTCTCCAGT 
TTGTCTCACC TGAACTCTTC CACTTCTGAC TATGGCACTG CCATGTCCTG CTCCACCTTG 
CTATACGGTA GGACCTGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015142759.1
CDS100..1200
Translation

Target ORF information:

RefSeq Version XM_015287273.1
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015287273.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTCCACG CCCTGAGCAG CTGGAGGAGC AACTGGGCGT GCGCTGCGGA GGGCCGCCGA 
ATCATGTCGA TGAGCCCAAA GCATACGACT CCTTTCTCAG TGTCTGACAT CTTGAGTCCT 
TTGGAGGAAA GCTACAAGAA AGTGGGCATG GAGGGCAGTA ACTTGGGGGC TCCCTTGTCA 
GCCTACAGAC AGTCTCAGGT TTCGCAACCG GCCATGCAGC AACACCCCAT GGGCCACAAC 
GGAACGGTGA CTGCCGCCTA CCATATGACA GCGGCAGGGG TCCCCCAGCT CTCCCACGCT 
ACGATGGGGG GCTACTGCAA TGGGAACCTG GGCAACATGA GCGAGCTCCC GCCTTACCAG 
GACACCATGC GGAACAGCGC TTCGGCGACA GGATGGTACG GCACCAACCC GGACCCCCGC 
TTCTCCTCAA TCTCCCGCTT CATGGCGCCG TCCTCGGGCA TGAACATGGG CGGCATGGGC 
GGCCTGAGCT CCCTGGGGGA CGTGAGCAAG AGCATGGCCC CGCTGCAGAG CACGCCGCGG 
AGGAAACGGA GGGTCCTGTT TTCCCAGGCC CAGGTTTACG AGCTGGAGAG ACGTTTCAAG 
CAGCAGAAAT ACCTCTCCGC CCCCGAGAGG GAACATTTAG CCAGCATGAT CCACCTCACC 
CCGACTCAGG TCAAAATCTG GTTCCAGAAC CACCGCTACA AGATGAAGCG GCAGGCCAAA 
GACAAGGCTG CGCAGCAGCA GATGCAACAG GAGAACGGCT CTTGCCAGCA GCAGCAGTCC 
CCCAGGAGGG TGGCGGTGCC GGTGCTGGTG AAGGATGGCA AGCCCTGCCA GGCGGGCTCC 
AACACCCCCA CCGCAGCCAT CCAGAGCCAC CCGCAGCAGG CGGCCACGAC CATCACGGTG 
GCCACCAATG GCAACAGCCT CGGACAGCAT CAGAGCCATC AGACAAACAG TGCGGGGCAG 
TCTCCCGACA TGGGACAGCA CTCGGCCAGC CCTTCCTCCC TGCAGAGCCA AGTCTCCAGT 
TTGTCTCACC TGAACTCTTC CACTTCTGAC TATGGCACTG CCATGTCCTG CTCCACCTTG 
CTATACGGTA GGACCTGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36750
Clone ID Related Accession (Same CDS sequence) XM_015287273.1 , XM_015287273.2
Accession Version XM_015287273.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1101bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTCCACG CCCTGAGCAG CTGGAGGAGC AACTGGGCGT GCGCTGCGGA GGGCCGCCGA 
ATCATGTCGA TGAGCCCAAA GCATACGACT CCTTTCTCAG TGTCTGACAT CTTGAGTCCT 
TTGGAGGAAA GCTACAAGAA AGTGGGCATG GAGGGCAGTA ACTTGGGGGC TCCCTTGTCA 
GCCTACAGAC AGTCTCAGGT TTCGCAACCG GCCATGCAGC AACACCCCAT GGGCCACAAC 
GGAACGGTGA CTGCCGCCTA CCATATGACA GCGGCAGGGG TCCCCCAGCT CTCCCACGCT 
ACGATGGGGG GCTACTGCAA TGGGAACCTG GGCAACATGA GCGAGCTCCC GCCTTACCAG 
GACACCATGC GGAACAGCGC TTCGGCGACA GGATGGTACG GCACCAACCC GGACCCCCGC 
TTCTCCTCAA TCTCCCGCTT CATGGCGCCG TCCTCGGGCA TGAACATGGG CGGCATGGGC 
GGCCTGAGCT CCCTGGGGGA CGTGAGCAAG AGCATGGCCC CGCTGCAGAG CACGCCGCGG 
AGGAAACGGA GGGTCCTGTT TTCCCAGGCC CAGGTTTACG AGCTGGAGAG ACGTTTCAAG 
CAGCAGAAAT ACCTCTCCGC CCCCGAGAGG GAACATTTAG CCAGCATGAT CCACCTCACC 
CCGACTCAGG TCAAAATCTG GTTCCAGAAC CACCGCTACA AGATGAAGCG GCAGGCCAAA 
GACAAGGCTG CGCAGCAGCA GATGCAACAG GAGAACGGCT CTTGCCAGCA GCAGCAGTCC 
CCCAGGAGGG TGGCGGTGCC GGTGCTGGTG AAGGATGGCA AGCCCTGCCA GGCGGGCTCC 
AACACCCCCA CCGCAGCCAT CCAGAGCCAC CCGCAGCAGG CGGCCACGAC CATCACGGTG 
GCCACCAATG GCAACAGCCT CGGACAGCAT CAGAGCCATC AGACAAACAG TGCGGGGCAG 
TCTCCCGACA TGGGACAGCA CTCGGCCAGC CCTTCCTCCC TGCAGAGCCA AGTCTCCAGT 
TTGTCTCACC TGAACTCTTC CACTTCTGAC TATGGCACTG CCATGTCCTG CTCCACCTTG 
CTATACGGTA GGACCTGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015142759.1
CDS2756..3856
Translation

Target ORF information:

RefSeq Version XM_015287273.2
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015287273.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTCCACG CCCTGAGCAG CTGGAGGAGC AACTGGGCGT GCGCTGCGGA GGGCCGCCGA 
ATCATGTCGA TGAGCCCAAA GCATACGACT CCTTTCTCAG TGTCTGACAT CTTGAGTCCT 
TTGGAGGAAA GCTACAAGAA AGTGGGCATG GAGGGCAGTA ACTTGGGGGC TCCCTTGTCA 
GCCTACAGAC AGTCTCAGGT TTCGCAACCG GCCATGCAGC AACACCCCAT GGGCCACAAC 
GGAACGGTGA CTGCCGCCTA CCATATGACA GCGGCAGGGG TCCCCCAGCT CTCCCACGCT 
ACGATGGGGG GCTACTGCAA TGGGAACCTG GGCAACATGA GCGAGCTCCC GCCTTACCAG 
GACACCATGC GGAACAGCGC TTCGGCGACA GGATGGTACG GCACCAACCC GGACCCCCGC 
TTCTCCTCAA TCTCCCGCTT CATGGCGCCG TCCTCGGGCA TGAACATGGG CGGCATGGGC 
GGCCTGAGCT CCCTGGGGGA CGTGAGCAAG AGCATGGCCC CGCTGCAGAG CACGCCGCGG 
AGGAAACGGA GGGTCCTGTT TTCCCAGGCC CAGGTTTACG AGCTGGAGAG ACGTTTCAAG 
CAGCAGAAAT ACCTCTCCGC CCCCGAGAGG GAACATTTAG CCAGCATGAT CCACCTCACC 
CCGACTCAGG TCAAAATCTG GTTCCAGAAC CACCGCTACA AGATGAAGCG GCAGGCCAAA 
GACAAGGCTG CGCAGCAGCA GATGCAACAG GAGAACGGCT CTTGCCAGCA GCAGCAGTCC 
CCCAGGAGGG TGGCGGTGCC GGTGCTGGTG AAGGATGGCA AGCCCTGCCA GGCGGGCTCC 
AACACCCCCA CCGCAGCCAT CCAGAGCCAC CCGCAGCAGG CGGCCACGAC CATCACGGTG 
GCCACCAATG GCAACAGCCT CGGACAGCAT CAGAGCCATC AGACAAACAG TGCGGGGCAG 
TCTCCCGACA TGGGACAGCA CTCGGCCAGC CCTTCCTCCC TGCAGAGCCA AGTCTCCAGT 
TTGTCTCACC TGAACTCTTC CACTTCTGAC TATGGCACTG CCATGTCCTG CTCCACCTTG 
CTATACGGTA GGACCTGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36751
Clone ID Related Accession (Same CDS sequence) XM_015287274.2 , XM_025150545.1 , XM_025150547.1 , XM_015287274.1 , XM_025150546.1
Accession Version XM_015287274.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015142760.1
CDS4082..5119
Translation

Target ORF information:

RefSeq Version XM_015287274.2
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015287274.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36751
Clone ID Related Accession (Same CDS sequence) XM_015287274.2 , XM_025150545.1 , XM_025150547.1 , XM_015287274.1 , XM_025150546.1
Accession Version XM_025150545.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_025006313.1
CDS4161..5198
Translation

Target ORF information:

RefSeq Version XM_025150545.1
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025150545.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36751
Clone ID Related Accession (Same CDS sequence) XM_015287274.2 , XM_025150545.1 , XM_025150547.1 , XM_015287274.1 , XM_025150546.1
Accession Version XM_025150547.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_025006315.1
CDS60..1097
Translation

Target ORF information:

RefSeq Version XM_025150547.1
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025150547.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36751
Clone ID Related Accession (Same CDS sequence) XM_015287274.2 , XM_025150545.1 , XM_025150547.1 , XM_015287274.1 , XM_025150546.1
Accession Version XM_015287274.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015142760.1
CDS456..1493
Translation

Target ORF information:

RefSeq Version XM_015287274.1
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015287274.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa36751
Clone ID Related Accession (Same CDS sequence) XM_015287274.2 , XM_025150545.1 , XM_025150547.1 , XM_015287274.1 , XM_025150546.1
Accession Version XM_025150546.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_025006314.1
CDS2898..3935
Translation

Target ORF information:

RefSeq Version XM_025150546.1
Organism Gallus gallus(chicken)
Definition Gallus gallus NK2 homeobox 1 (NKX2-1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025150546.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGTCGATGA GCCCAAAGCA TACGACTCCT TTCTCAGTGT CTGACATCTT GAGTCCTTTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCAGTAACT TGGGGGCTCC CTTGTCAGCC 
TACAGACAGT CTCAGGTTTC GCAACCGGCC ATGCAGCAAC ACCCCATGGG CCACAACGGA 
ACGGTGACTG CCGCCTACCA TATGACAGCG GCAGGGGTCC CCCAGCTCTC CCACGCTACG 
ATGGGGGGCT ACTGCAATGG GAACCTGGGC AACATGAGCG AGCTCCCGCC TTACCAGGAC 
ACCATGCGGA ACAGCGCTTC GGCGACAGGA TGGTACGGCA CCAACCCGGA CCCCCGCTTC 
TCCTCAATCT CCCGCTTCAT GGCGCCGTCC TCGGGCATGA ACATGGGCGG CATGGGCGGC 
CTGAGCTCCC TGGGGGACGT GAGCAAGAGC ATGGCCCCGC TGCAGAGCAC GCCGCGGAGG 
AAACGGAGGG TCCTGTTTTC CCAGGCCCAG GTTTACGAGC TGGAGAGACG TTTCAAGCAG 
CAGAAATACC TCTCCGCCCC CGAGAGGGAA CATTTAGCCA GCATGATCCA CCTCACCCCG 
ACTCAGGTCA AAATCTGGTT CCAGAACCAC CGCTACAAGA TGAAGCGGCA GGCCAAAGAC 
AAGGCTGCGC AGCAGCAGAT GCAACAGGAG AACGGCTCTT GCCAGCAGCA GCAGTCCCCC 
AGGAGGGTGG CGGTGCCGGT GCTGGTGAAG GATGGCAAGC CCTGCCAGGC GGGCTCCAAC 
ACCCCCACCG CAGCCATCCA GAGCCACCCG CAGCAGGCGG CCACGACCAT CACGGTGGCC 
ACCAATGGCA ACAGCCTCGG ACAGCATCAG AGCCATCAGA CAAACAGTGC GGGGCAGTCT 
CCCGACATGG GACAGCACTC GGCCAGCCCT TCCTCCCTGC AGAGCCAAGT CTCCAGTTTG 
TCTCACCTGA ACTCTTCCAC TTCTGACTAT GGCACTGCCA TGTCCTGCTC CACCTTGCTA 
TACGGTAGGA CCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Demarcation of ventral territories by the homeobox gene NKX2.1 during early chick development.
Development genes and evolution208(3)168-71(1998 May)
Pera EM,Kessel M


book

Control of anteroposterior and dorsoventral domains of Nkx-6.1 gene expression relative to other Nkx genes during vertebrate CNS development.
Mechanisms of development72(1-2)77-88(1998 Mar)
Qiu M,Shimamura K,Sussel L,Chen S,Rubenstein JL