MMP9 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Gene Symbol	MMP9
Entrez Gene ID	395387
Full Name	matrix metallopeptidase 9
General protein information	Preferred Names matrix metallopeptidase 9 Names matrix metalloproteinase-9 75 kDa gelatinase matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA Protein Name

NM_204667.1 NP_989998.1 matrix metalloproteinase-9 precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_204667.1	NP_989998.1	matrix metalloproteinase-9 precursor

Homologies

Bos taurus (cattle)	MMP9	NP_777169.1
Rattus norvegicus (Norway rat)	Mmp9	NP_112317.1
Danio rerio (zebrafish)	mmp9	NP_998288.1
Xenopus tropicalis (tropical clawed frog)	LOC100494738	XP_002932554.1
Pan troglodytes (chimpanzee)	MMP9	XP_003317021.1
Mus musculus (house mouse)	Mmp9	NP_038627.1
	MMP9	XP_001104871.1
Gallus gallus (chicken)	MMP9	NP_989998.1
Xenopus tropicalis (tropical clawed frog)	mmp9	NP_001006843.1
Homo sapiens (human)	MMP9	NP_004985.2
Canis lupus familiaris (dog)	MMP9	NP_001003219.1
Arabidopsis thaliana (thale cress)	AT2G45040	NP_182030.1

Gene Ontology
Bibliography

Related articles in PubMed

Matrix metalloproteinases-2 and -9 in Campylobacter jejuni-induced paralytic neuropathy resembling Guillain-Barr? syndrome in chickens.
Nyati KK, Prasad KN, Agrawal V, Husain N
Microbial pathogenesis111395-401(2017 Oct)

Glucocorticoid Effects on Cerebellar Development in a Chicken Embryo Model: Exploring Changes in PAX6 and Metalloproteinase-9 After Exposure to Dexamethasone.
Austdal LP, Bj?rnstad S, Mathisen GH, Aden PK, Mikkola I, Paulsen RE, Rakkestad KE
Journal of neuroendocrinology28(12)(2016 Dec)

Involvement of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct regression and recrudescence.
Le?niak-Walentyn A, Hrabia A
Cell and tissue research366(2)443-454(2016 Nov)

Expression and localization of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct during maturation.
Le?niak-Walentyn A, Hrabia A
Cell and tissue research364(1)185-97(2016 Apr)

Type I collagen accelerates the spreading of lens epithelial cells through the expression and activation of matrix metalloproteinases.
Shimada A, Miyata Y, Kosano H
Current eye research39(5)460-71(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

metalloproteinase-9 increased as a result of glucocorticoid-stimulation in vitro. Findings indicate that excess prenatal glucocorticoid stimulation disturbs normal development of the cerebellum through mechanisms associated with reduced proliferation and accelerated maturation where PAX6 and metalloproteinase-9 play important roles.
Title: Glucocorticoid Effects on Cerebellar Development in a Chicken Embryo Model: Exploring Changes in PAX6 and Metalloproteinase-9 After Exposure to Dexamethasone.

Real-time polymerase chain reaction and western blot revealed different expression of MMP-2, -7, -9 and TIMP-2 and -3 on mRNA and protein levels, respectively, as well as activity of MMP-2 and -9 by activity assay in the oviduct parts.
Title: Involvement of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct regression and recrudescence.

Protein level of MMP-9 was diminished, while MMP-7 and TIMP-3 were elevated in the oviduct of growing birds. MMP-2 and TIMP-2 protein levels remained constant, with a slight increase in MMP-2 concentration just before reaching maturity.
Title: Expression and localization of matrix metalloproteinases (MMP-2, -7, -9) and their tissue inhibitors (TIMP-2, -3) in the chicken oviduct during maturation.

Type I collagen promotes the expression and activation of MMPs in a lens epithelial cell-specific manner.
Title: Type I collagen accelerates the spreading of lens epithelial cells through the expression and activation of matrix metalloproteinases.

MMP9 is a novel effector which is required for neural crest cells delamination and migration.
Title: Matrix metalloproteinase 9/gelatinase B is required for neural crest cell migration.

The following MMP9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa24470	NM_204667.1 Latest version!	Gallus gallus matrix metallopeptidase 9 (MMP9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MMP9 ( NM_204667.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989998.1 Gallus gallus matrix metallopeptidase 9 (MMP9), mRNA.

Price & Availability↑

CloneID	OGa24470
Clone ID Related Accession (Same CDS sequence)	NM_204667.1
Accession Version	NM_204667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Gallus gallus(chicken)
Product	matrix metalloproteinase-9 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF222690.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF222690.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTCCTGG CCCCGCTCAC CCTGGCACTG CTGGCTCTCT GCCGCGCCGC CCCACTGCAC 
AGCAAGCCGC AGGCGGTCAT CACCTTCCCA GGGGAGCTGC TCAGCGCCCC ATCAGACGTG 
GAGCTGGCGG AGAACTACCT GCTGCGCTTC GGCTACATCC AGGAGGCAGA GGTGAGGAGG 
AGCAGCAAGC ACGTGTCCCT GGCCAAAGCG CTGCGCAGGA TGCAGAAGCA GCTGGGGCTG 
GAGGAGACGG GGGAGCTGGA CGCCAGCACC CTGGAGGCCA TGCGAGCCCC CCGCTGTGGG 
GTGCCTGACG TGGGGGGTTT CCTCACCTTC GAGGGGGAGC TCAAATGGGA CCACATGGAC 
CTCACGTACC GGGTGATGAA CTACTCCCCC GACCTGGACC GTGCCGTGAT AGATGATGCC 
TTCCGGCGGG CATTCAAGGT GTGGAGTGAT GTCACTCCCC TCACCTTCAC CCAGATTTAC 
AGCGGCGAGG CAGACATCAT GATCATGTTC GGCAGCCAAG AGCATGGTGA TGGGTACCCC 
TTCGACGGCA AGGATGGGCT CCTGGCCCAC GCCTTTCCCC CCGGCAGTGG GATTCAGGGC 
GATGCCCACT TCGATGATGA TGAGTTCTGG ACTCTGGGAA CCGGCTTAGA GGTGAAGACC 
CGCTATGGGA ATGCCAACGG GGCCAGCTGC CACTTCCCCT TCATCTTTGA GGGCCGCTCC 
TACTCCCGGT GCATCACGGA GGGCCGCACG GATGGGATGC TGTGGTGTGC CACCACCGCC 
AGCTACGACG CCGACAAGAC CTACGGCTTC TGCCCCAGCG AGCTGCTCTA CACCAATGGT 
GGCAACAGCG ATGGGTCTCC CTGCGTCTTC CCCTTCATCT TCGATGGCGC CTCCTATGAC 
ACCTGCACCA CAGATGGGCG CTCTGACGGC TATCGCTGGT GTGCCACCAC GGCCAACTTC 
GACCAGGACA AGAAATACGG CTTCTGCCCC AACCGAGACA CGGCGGCGAT CGGTGGCAAC 
TCCCAGGGGG ACCCGTGTGT CTTCCCCTTC ACCTTCCTGG GGCAGTCCTA CAGCGCGCGC 
ACCAGCCAGG GCCGGCAGGA CGGGAAGCTC TGGTGTGCCA CCACCAGCAA CTATGACACC 
GACAAGAAGT GGGGCTTCTG CCCAGACAGA GGTTACAGCA TCTTCTTGGT GGCTGCCCAC 
GAGTTTGGGC ACTCACTGGG GCTGGACCAC TCCAGCGTGC GCGAGGCATT GATGTACCCT 
ATGTACAGCT ACGTCCAGGA CTTCCAGCTG CATGAGGATG ATGTCCAGGG CATCCAGTAC 
CTCTATGGTC GTGGCTCTGG CCCTGAGCCC ACCCCCCCGG CACCTTTGCC CACCGAGGAG 
CCCCAGTCCA TACCCACCGA AGCTGGCAGT GCTTCCACCA CAGAGGAGGA GGAGGAGGAG 
ACACCTGAGC CCACAGCTGA GCCCAGCCCC GTGGACCCCA GCCGGGATGC CTGCATGGAG 
AAGAACTTCG ACGCCATCAC TGAGATCAAT GGAGAGCTGC ACTTCTTCAA GAATGGGAAA 
TACTGGACCC ACTCGTCCTT CTGGAAATCA GGCACTCAGG GCGCCTTCTC TATCGCTGAC 
ACCTGGCCCG GCCTCCCGGC TGTCATCGAC GCGGCGTTCC AAGATGTGCT CACCAAGAGG 
GTCTTCTTCT TCGCGGGACG GCAGTTCTGG GTGTTCTCCG GCAAGAACGC AGTGGGCCCC 
CGTAGGATTG AGAAGTTGGG CATTGGGAAG GAGGCCGGGC GCATCACGGG GGCCCTGCAG 
CGGGGACGTG GCAAAGTGCT GCTCTTCAGT GGGGAGCACT ACTGGAGGCT GGACGTGAAG 
GTCCAGACAG TGGACAAGGG CTACCCCCGT GACACTGATG ATGTCTTTAC TGGTGTCCCC 
CTTGACGCAC GTAACGTCTT CCTGTACCAA GACAAGTACC ACTTCTGCCG GGACAGCTTC 
TACTGGAGGA TGACCCCACG TTACCAGGTG GACCGCGTGG GATACATCAG ATACGACCTC 
CTGCAGTGCC CCCAGCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989998.1
CDS	1..2061
Translation	MLLAPLTLALLALCRAAPLHSKPQAVITFPGELLSAPSDVELAENYLLRFGYIQEAEVRRSSKHVSLAKALRRMQKQLGLEETGELDASTLEAMRAPRCGVPDVGGFLTFEGELKWDHMDLTYRVMNYSPDLDRAVIDDAFRRAFKVWSDVTPLTFTQIYSGEADIMIMFGSQEHGDGYPFDGKDGLLAHAFPPGSGIQGDAHFDDDEFWTLGTGLEVKTRYGNANGASCHFPFIFEGRSYSRCITEGRTDGMLWCATTASYDADKTYGFCPSELLYTNGGNSDGSPCVFPFIFDGASYDTCTTDGRSDGYRWCATTANFDQDKKYGFCPNRDTAAIGGNSQGDPCVFPFTFLGQSYSARTSQGRQDGKLWCATTSNYDTDKKWGFCPDRGYSIFLVAAHEFGHSLGLDHSSVREALMYPMYSYVQDFQLHEDDVQGIQYLYGRGSGPEPTPPAPLPTEEPQSIPTEAGSASTTEEEEEETPEPTAEPSPVDPSRDACMEKNFDAITEINGELHFFKNGKYWTHSSFWKSGTQGAFSIADTWPGLPAVIDAAFQDVLTKRVFFFAGRQFWVFSGKNAVGPRRIEKLGIGKEAGRITGALQRGRGKVLLFSGEHYWRLDVKVQTVDKGYPRDTDDVFTGVPLDARNVFLYQDKYHFCRDSFYWRMTPRYQVDRVGYIRYDLLQCPQH

Target ORF information:

RefSeq Version	NM_204667.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus matrix metallopeptidase 9 (MMP9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204667.1

ORF Insert Sequence:

ATGCTCCTGG CCCCGCTCAC CCTGGCACTG CTGGCTCTCT GCCGCGCCGC CCCACTGCAC 
AGCAAGCCGC AGGCGGTCAT CACCTTCCCA GGGGAGCTGC TCAGCGCCCC ATCAGACGTG 
GAGCTGGCGG AGAACTACCT GCTGCGCTTC GGCTACATCC AGGAGGCAGA GGTGAGGAGG 
AGCAGCAAGC ACGTGTCCCT GGCCAAAGCG CTGCGCAGGA TGCAGAAGCA GCTGGGGCTG 
GAGGAGACGG GGGAGCTGGA CGCCAGCACC CTGGAGGCCA TGCGAGCCCC CCGCTGTGGG 
GTGCCTGACG TGGGGGGTTT CCTCACCTTC GAGGGGGAGC TCAAATGGGA CCACATGGAC 
CTCACGTACC GGGTGATGAA CTACTCCCCC GACCTGGACC GTGCCGTGAT AGATGATGCC 
TTCCGGCGGG CATTCAAGGT GTGGAGTGAT GTCACTCCCC TCACCTTCAC CCAGATTTAC 
AGCGGCGAGG CAGACATCAT GATCATGTTC GGCAGCCAAG AGCATGGTGA TGGGTACCCC 
TTCGACGGCA AGGATGGGCT CCTGGCCCAC GCCTTTCCCC CCGGCAGTGG GATTCAGGGC 
GATGCCCACT TCGATGATGA TGAGTTCTGG ACTCTGGGAA CCGGCTTAGA GGTGAAGACC 
CGCTATGGGA ATGCCAACGG GGCCAGCTGC CACTTCCCCT TCATCTTTGA GGGCCGCTCC 
TACTCCCGGT GCATCACGGA GGGCCGCACG GATGGGATGC TGTGGTGTGC CACCACCGCC 
AGCTACGACG CCGACAAGAC CTACGGCTTC TGCCCCAGCG AGCTGCTCTA CACCAATGGT 
GGCAACAGCG ATGGGTCTCC CTGCGTCTTC CCCTTCATCT TCGATGGCGC CTCCTATGAC 
ACCTGCACCA CAGATGGGCG CTCTGACGGC TATCGCTGGT GTGCCACCAC GGCCAACTTC 
GACCAGGACA AGAAATACGG CTTCTGCCCC AACCGAGACA CGGCGGCGAT CGGTGGCAAC 
TCCCAGGGGG ACCCGTGTGT CTTCCCCTTC ACCTTCCTGG GGCAGTCCTA CAGCGCGCGC 
ACCAGCCAGG GCCGGCAGGA CGGGAAGCTC TGGTGTGCCA CCACCAGCAA CTATGACACC 
GACAAGAAGT GGGGCTTCTG CCCAGACAGA GGTTACAGCA TCTTCTTGGT GGCTGCCCAC 
GAGTTTGGGC ACTCACTGGG GCTGGACCAC TCCAGCGTGC GCGAGGCATT GATGTACCCT 
ATGTACAGCT ACGTCCAGGA CTTCCAGCTG CATGAGGATG ATGTCCAGGG CATCCAGTAC 
CTCTATGGTC GTGGCTCTGG CCCTGAGCCC ACCCCCCCGG CACCTTTGCC CACCGAGGAG 
CCCCAGTCCA TACCCACCGA AGCTGGCAGT GCTTCCACCA CAGAGGAGGA GGAGGAGGAG 
ACACCTGAGC CCACAGCTGA GCCCAGCCCC GTGGACCCCA GCCGGGATGC CTGCATGGAG 
AAGAACTTCG ACGCCATCAC TGAGATCAAT GGAGAGCTGC ACTTCTTCAA GAATGGGAAA 
TACTGGACCC ACTCGTCCTT CTGGAAATCA GGCACTCAGG GCGCCTTCTC TATCGCTGAC 
ACCTGGCCCG GCCTCCCGGC TGTCATCGAC GCGGCGTTCC AAGATGTGCT CACCAAGAGG 
GTCTTCTTCT TCGCGGGACG GCAGTTCTGG GTGTTCTCCG GCAAGAACGC AGTGGGCCCC 
CGTAGGATTG AGAAGTTGGG CATTGGGAAG GAGGCCGGGC GCATCACGGG GGCCCTGCAG 
CGGGGACGTG GCAAAGTGCT GCTCTTCAGT GGGGAGCACT ACTGGAGGCT GGACGTGAAG 
GTCCAGACAG TGGACAAGGG CTACCCCCGT GACACTGATG ATGTCTTTAC TGGTGTCCCC 
CTTGACGCAC GTAACGTCTT CCTGTACCAA GACAAGTACC ACTTCTGCCG GGACAGCTTC 
TACTGGAGGA TGACCCCACG TTACCAGGTG GACCGCGTGG GATACATCAG ATACGACCTC 
CTGCAGTGCC CCCAGCATTA G