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SPRY2 cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol SPRY2
Entrez Gene ID 395584
Full Name sprouty RTK signaling antagonist 2
General protein information
Preferred Names

sprouty RTK signaling antagonist 2

Names

protein sprouty homolog 2
sprouty 2
sprouty homolog 2
spry-2
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015275115.1 XP_015130601.1 protein sprouty homolog 2 isoform X1
XM_015275115.2 XP_015130601.1 protein sprouty homolog 2 isoform X1
XM_015275119.1 XP_015130605.1 protein sprouty homolog 2 isoform X1
XM_015275119.2 XP_015130605.1 protein sprouty homolog 2 isoform X1
XM_015275123.2 XP_015130609.1 protein sprouty homolog 2 isoform X1
XM_015275123.1 XP_015130609.1 protein sprouty homolog 2 isoform X1
NM_204800.1 NP_990131.1 protein sprouty homolog 2

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SPRY2 XP_003952460.1
Bos taurus (cattle) SPRY2 NP_001069615.1
SPRY2 XP_001090386.2
Canis lupus familiaris (dog) SPRY2 XP_005634066.1
Rattus norvegicus (Norway rat) Spry2 NP_001012046.1
Mus musculus (house mouse) Spry2 NP_036027.1
Danio rerio (zebrafish) spry2 NP_001003842.2
Xenopus tropicalis (tropical clawed frog) spry2 NP_001006932.2
Homo sapiens (human) SPRY2 NP_005833.1
Gallus gallus (chicken) SPRY2 NP_990131.1

Gene Ontology


Bibliography

Related articles in PubMed

Sprouty2 mediated tuning of signalling is essential for somite myogenesis.
Abu-Elmagd M, Goljanek Whysall K, Wheeler G, M?nsterberg A
BMC medical genomics8 Suppl 1S8(2015)

Activation of Ras-ERK pathway by Fgf8 and its downregulation by Sprouty2 for the isthmus organizing activity.
Suzuki-Hirano A, Harada H, Sato T, Nakamura H
Developmental biology337(2)284-93(2010 Jan)

Stage-dependent craniofacial defects resulting from Sprouty2 overexpression.
Goodnough LH, Brugmann SA, Hu D, Helms JA
Developmental dynamics : an official publication of the American Association of Anatomists236(7)1918-28(2007 Jul)

Regulation of isthmic Fgf8 signal by sprouty2.
Suzuki-Hirano A, Sato T, Nakamura H
Development (Cambridge, England)132(2)257-65(2005 Jan)

Differential display of genes expressed at the midbrain - hindbrain junction identifies sprouty2: an FGF8-inducible member of a family of intracellular FGF antagonists.
Chambers D, Medhurst AD, Walsh FS, Price J, Mason I
Molecular and cellular neurosciences15(1)22-35(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spry2 function during chick somite myogenesis
Title: Sprouty2 mediated tuning of signalling is essential for somite myogenesis.

'Prolonged' activation of ERK by misexpression of Fgf8b and dominant-negative Sprouty2 (dnSprouty2) did not change the fate of the mesencephalic alar plate.
Title: Activation of Ras-ERK pathway by Fgf8 and its downregulation by Sprouty2 for the isthmus organizing activity.

Sprouty2 plays a role in the outgrowth of facial prominences independent of canonical fibroblast growth factor signaling.
Title: Stage-dependent craniofacial defects resulting from Sprouty2 overexpression.

Sprouty2 may regulate the Fgf8-Ras-ERK signaling pathway for the proper regionalization of the metencephalon and mesencephalon
Title: Regulation of isthmic Fgf8 signal by sprouty2.

The following SPRY2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRY2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa27020 NM_204800.1
Latest version!		 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OGa30792 XM_015275119.2
Latest version!		 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OGa30792 XM_015275115.2
Latest version!		 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OGa30792 XM_015275123.2
Latest version!		 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
View Old Accession Versions >>
OGa30792 XM_015275115.1 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OGa30792 XM_015275119.1 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OGa30792 XM_015275123.1 Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa27020
Clone ID Related Accession (Same CDS sequence) NM_204800.1
Accession Version NM_204800.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-15
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF176904.1. ##Evidence-Data-START## Transcript exon combination :: AF176904.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG CGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT CTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_990131.1
CDS156..1097
Translation

Target ORF information:

RefSeq Version NM_204800.1
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_204800.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG CGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT CTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa30792
Clone ID Related Accession (Same CDS sequence) XM_015275115.1 , XM_015275115.2 , XM_015275119.1 , XM_015275119.2 , XM_015275123.2 , XM_015275123.1
Accession Version XM_015275115.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455757.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015130601.1
CDS175..1116
Translation

Target ORF information:

RefSeq Version XM_015275115.1
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015275115.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa30792
Clone ID Related Accession (Same CDS sequence) XM_015275115.1 , XM_015275115.2 , XM_015275119.1 , XM_015275119.2 , XM_015275123.2 , XM_015275123.1
Accession Version XM_015275115.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015275115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015130601.1
CDS186..1127
Translation

Target ORF information:

RefSeq Version XM_015275115.2
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015275115.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa30792
Clone ID Related Accession (Same CDS sequence) XM_015275115.1 , XM_015275115.2 , XM_015275119.1 , XM_015275119.2 , XM_015275123.2 , XM_015275123.1
Accession Version XM_015275119.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455757.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015130605.1
CDS180..1121
Translation

Target ORF information:

RefSeq Version XM_015275119.1
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015275119.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa30792
Clone ID Related Accession (Same CDS sequence) XM_015275115.1 , XM_015275115.2 , XM_015275119.1 , XM_015275119.2 , XM_015275123.2 , XM_015275123.1
Accession Version XM_015275119.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015275119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015130605.1
CDS196..1137
Translation

Target ORF information:

RefSeq Version XM_015275119.2
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015275119.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa30792
Clone ID Related Accession (Same CDS sequence) XM_015275115.1 , XM_015275115.2 , XM_015275119.1 , XM_015275119.2 , XM_015275123.2 , XM_015275123.1
Accession Version XM_015275123.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015275123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015130609.1
CDS146..1087
Translation

Target ORF information:

RefSeq Version XM_015275123.2
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015275123.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa30792
Clone ID Related Accession (Same CDS sequence) XM_015275115.1 , XM_015275115.2 , XM_015275119.1 , XM_015275119.2 , XM_015275123.2 , XM_015275123.1
Accession Version XM_015275123.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455757.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015130609.1
CDS136..1077
Translation

Target ORF information:

RefSeq Version XM_015275123.1
Organism Gallus gallus(chicken)
Definition Gallus gallus sprouty RTK signaling antagonist 2 (SPRY2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015275123.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGACGA GAGTTCAGCA CGGCAGCGGG TCACAGGCCT TGCTACAGGC TCGGCGTGAC 
AGTGGGAGGC CGCATGGGGA GCCTGACCTG CGGGATGTCC TGACGCAGCA AGTCCACATC 
CTGTCCCTGG ACCAGATCAG AGCTATCCGC AACACGAATG AGTACACAGA GGGACCGACG 
GTGGCTCCGC GGCCAGGGGT CAAGTCAGCT CCTCGTTCAG TGAGCCAGCC CAAAAGTGAG 
AGGCCCCATG GCCTGCCTGA GCATCGCCAT TTTGGCCGGG TTCAGCACAC GCAAACACAC 
GCTTCTCCTC GGGCACCTCT GTCCCGCTCC ATCAGCACAG TCAGCACGGG CTCACGGAGC 
AGTACAAGGA CAAGTACGAG CAGTAATTCC TCTGAACAAA GACTTCTAGG ATCATCTTCA 
GGGCCAGTTG CTGACGGGAT AGTCCGCATG CAGCCCAAGT CTGAGCTCAA GTCAAGCGAG 
CTGAAGCCGC TGAGCAAAGA AGACTTGGGA GCACACAGCT ACAGGTGTGA GGACTGTGGG 
AAGTGCAAGT GCAAGGAGTG CACGTATCCA AGGACCCTCC CATCGTGTTG GATCTGTGAC 
AAACAGTGTC TTTGCTCAGC CCAAAACGTG GTCGATTACG GGACTTGTGT GTGCTGTGTG 
AAGGGCCTCT TCTATCACTG CTCCAACGAT GATGAGGACA ACTGCGCTGA CAACCCCTGC 
TCTTGCAGTC AGTCGCACTG CTGCACTAGG TGGTCAGCCA TGGGTGTGGT GTCCCTCTTC 
CTGCCTTGCT TGTGGTGTTA CCTGCCAGCC AAGGGTTGCC TTAAGTTGTG CCAGGGCTGT 
TACGACCGGG TCAATCGGCC TGGGTGCCGC TGTAAACACT CCAACACGGT TTGCTGCAAA 
GTTCCCAGTG TCCCCCCCAG GAACTTTGAA AAGCCAACGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Sprouty2 mediated tuning of signalling is essential for somite myogenesis.
BMC medical genomics8 Suppl 1S8(2015)
Abu-Elmagd M,Goljanek Whysall K,Wheeler G,M?nsterberg A


book

Activation of Ras-ERK pathway by Fgf8 and its downregulation by Sprouty2 for the isthmus organizing activity.
Developmental biology337(2)284-93(2010 Jan)
Suzuki-Hirano A,Harada H,Sato T,Nakamura H


book

Stage-dependent craniofacial defects resulting from Sprouty2 overexpression.
Developmental dynamics : an official publication of the American Association of Anatomists236(7)1918-28(2007 Jul)
Goodnough LH,Brugmann SA,Hu D,Helms JA


book

Regulation of isthmic Fgf8 signal by sprouty2.
Development (Cambridge, England)132(2)257-65(2005 Jan)
Suzuki-Hirano A,Sato T,Nakamura H


book

Differential display of genes expressed at the midbrain - hindbrain junction identifies sprouty2: an FGF8-inducible member of a family of intracellular FGF antagonists.
Molecular and cellular neurosciences15(1)22-35(2000 Jan)
Chambers D,Medhurst AD,Walsh FS,Price J,Mason I