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NOTCH1 cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol NOTCH1
Entrez Gene ID 395655
Full Name notch 1
General protein information
Preferred Names

notch 1

Names

neurogenic locus notch homolog protein 1
NOTCH-1
Notch homolog 1, translocation-associated
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015279325.2 XP_015134811.1 neurogenic locus notch homolog protein 1 isoform X2
NM_001030295.1 NP_001025466.1 neurogenic locus notch homolog protein 1
XM_015279324.1 XP_015134810.1 neurogenic locus notch homolog protein 1 isoform X1
XM_015279325.1 XP_015134811.1 neurogenic locus notch homolog protein 1 isoform X2
XM_015279324.2 XP_015134810.1 neurogenic locus notch homolog protein 1 isoform X1

Pathways from BioSystems


Homologies


Bos taurus (cattle) NOTCH1 XP_002691666.2
Gallus gallus (chicken) NOTCH1 XP_415420.4
Pan troglodytes (chimpanzee) NOTCH1 XP_520371.2
LOC709947 XP_002808138.1
Rattus norvegicus (Norway rat) Notch1 NP_001099191.1
Drosophila melanogaster (fruit fly) N NP_001245510.1
Mus musculus (house mouse) Notch1 NP_032740.3
Canis lupus familiaris (dog) NOTCH1 XP_005625490.1
Homo sapiens (human) NOTCH1 NP_060087.3
Danio rerio (zebrafish) notch1b NP_571377.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001015 XP_309208.5
Xenopus tropicalis (tropical clawed frog) notch1 NP_001090757.1

Gene Ontology


Bibliography

Related articles in PubMed

Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis.
Esteves de Lima J, Bonnin MA, Birchmeier C, Duprez D
eLife5(2016 08)

Cytoplasmic NOTCH and membrane-derived ?-catenin link cell fate choice to epithelial-mesenchymal transition during myogenesis.
Sieiro D, Rios AC, Hirst CE, Marcelle C
eLife5(2016 05)

A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo.
Stasiulewicz M, Gray SD, Mastromina I, Silva JC, Bj?rklund M, Seymour PA, Booth D, Thompson C, Green RJ, Hall EA, Serup P, Dale JK
Development (Cambridge, England)142(13)2291-303(2015 Jul)

Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K
Development (Cambridge, England)141(8)1671-82(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results identify a novel mechanism acting downstream of muscle contraction, where YAP activates JAG2 expression in muscle fibers, which in turn regulates the pool of fetal muscle progenitors via NOTCH in a non-cell-autonomous manner.
Title: Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis.

Cytoplasmic NOTCH and membrane-derived beta-catenin link cell fate choice to epithelial-mesenchymal transition during myogenesis.
Title: Cytoplasmic NOTCH and membrane-derived ?-catenin link cell fate choice to epithelial-mesenchymal transition during myogenesis.

These data identify Notch signalling as a novel modulator of Shh signalling that acts mechanistically via regulation of ciliary localisation of key components of its transduction machinery.
Title: A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo.

These results identified the apical endfoot as the central site of active Notch signaling to securely prohibit inappropriate differentiation of neural progenitors.
Title: Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.

Mib1 is a major component of the signal-sending cells required to provide Notch ligand activity for specifying neurons and glia in the spinal cord
Title: The E3 ligase Mind bomb-1 (Mib1) modulates Delta-Notch signaling to control neurogenesis and gliogenesis in the developing spinal cord.

The following NOTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00576 NM_001030295.1
Latest version!		 Gallus gallus notch 1 (NOTCH1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1371.30
$1959.00
OGa43346 XM_015279324.2
Latest version!		 Gallus gallus notch 1 (NOTCH1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1371.30
$1959.00
OGa43347 XM_015279325.2
Latest version!		 Gallus gallus notch 1 (NOTCH1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1371.30
$1959.00
View Old Accession Versions >>
OGa43347 XM_015279325.1 Gallus gallus notch 1 (NOTCH1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1371.30
$1959.00
OGa43346 XM_015279324.1 Gallus gallus notch 1 (NOTCH1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa00576
Clone ID Related Accession (Same CDS sequence) NM_001030295.1
Accession Version NM_001030295.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-01
Organism Gallus gallus(chicken)
Product neurogenic locus notch homolog protein 1
Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AADN05000150.1. On May 28, 2014 this sequence version replaced XM_415420.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
ATGGGGCGGT GTAGTGCTGC CCATCCCCGC GGTGGGGTGC ATTGCCCCGG GCTCTGTGCT 
GTGCCTGACG CTCTCCTTCT GTTCCCAGGT GTGCGGTGCA CGCAGCTGGC CGAATCCTGC 
CTCAACGGGG GCAAGTGTGA AACTTTCCTC AATGGGACGG AGGTGTGCCA GTGCAGCAGT 
GCCCATATGG GCGAGCGGTG CCAGCTGCCC AACCCCTGCC TCAGCTCCCC CTGCAAGAAC 
GCCGGCACCT GCATCCCCCT GCTGCGTGGC AGCACCGCCG ACTACACCTG TGTCTGCCGC 
CTGGGCTTCA CCGACGAGCT GTGCCTCACA CCCCTGGACA ACGCCTGCCT CAACAACCCC 
TGCCGCAACG GGGGCACCTG TGACCTGGTG ACACTCAGCG AGTACAAGTG CCGCTGCCCG 
CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG GCCGACCCCT GTGCCTCCAA CCCCTGTGCC 
AACGGGGGTC AGTGCGTCCC CTTCGAAGCC CACTACATCT GCCGCTGCAC GGCCGGCTTC 
CACGGGGCCA ACTGCAAGCA GGACGTGAAC GAATGCAACA TCTCACCACC AGTCTGCAAG 
AACGGTGGCA GCTGCACCAA CGAGGTGGGC ACCTACCAGT GCTCCTGCAA GCCAGCCTAC 
ACCGGGCAGA ACTGCGAGCA TCTCTATGTG CCCTGCAACC CCTCGCCCTG CCAGAATGGG 
GGCACGTGCC GCCAAACTGG GGACACCACC TACGACTGCA CCTGTCTGCC AGGGTTCACC 
GGGCAGAACT GCGAGGAGAA CATCGATGAC TGTCCAGGGA ACAACTGCAG GAATGGGGGC 
ACCTGCGTGG ATGGCGTCAA CACCTACAAC TGCCAGTGCC CGCCCGAGTG GACAGGTCAG 
TACTGCACCG AGGATGTGGA TGAGTGCCAG CTGATGCCCA ACGCCTGCCA GAACGGGGGC 
ACCTGCCACA ACAACCACGG CGGCTACAAC TGCGTCTGCG TCAATGGCTG GACGGGTGAG 
GACTGCAGTG AGAACATCGA TGACTGCGCC ATGGCTGCCT GCTTCCAGGG GGCCACCTGC 
CATGACCGGG TGGCCTCCTT CTACTGCGAG TGTCCCCATG GGCGCACAGG TTTGCTGTGC 
CACCTGGATG ACGCCTGCAT CAGCAACCCC TGCAACGAGG GCTCCAACTG CGATACCAAC 
CCTGTCAATG GCAAAGCCAT CTGCACGTGT CCTTCGGGGT ACATGGGGCC GGCGTGCAAC 
CAGGACGTGG ATGAGTGCTC ACTGGGAGCC AACCCCTGTG AGCACGCAGG GAAATGCATC 
AACACCCAGG GGTCCTTCCA ATGCCAGTGT CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG 
ATCGATGTCA ATGAGTGCCT CTCCAACCCC TGCCAGAACG ATGCCACCTG CCTGGACCAG 
ATCGGGGAGT TCCAGTGCAT CTGCATGCCT GGATACGAGG GCGTCTACTG TGAAATCAAC 
ACAGATGAGT GTGCCAGCAG CCCCTGCCTG CACAATGGCA ACTGCCTGGA TAAGATCAAC 
GAGTTCCACT GCGAGTGCCC CACTGGGTTC AACGGGCACC TGTGCCAGTT TGACATCGAT 
GAGTGTGCCA GCACCCCCTG CAAGAATGGG GCCAAGTGCG TGGATGGCCC CAACACCTAC 
AGCTGCGAGT GCACCGAAGG TTTCTCAGGT GTTCACTGTG AGATCGACAT CGATGAGTGC 
AACCCCGACC CGTGCCACTA TGGGACCTGC AAGGACAGCA TCGCCGCCTT CACCTGCCTC 
TGCCAGCCCG GCTACACGGG CCACCGCTGC GACATCAACA TCAATGAGTG CCAGAGCCAG 
CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC AGGGACAACG CCTACAACTG TCTGTGCCTC 
AAGGGGACCA CGGGGCCCAA CTGCGAGATC AACCTGGACG ACTGCGCCAG CAACCCCTGC 
GACTACGGCA AGTGCATCGA CAAGATCAAC GGCTATGAGT GCACCTGTGA GCCGGGGTAC 
ACAGGGCGCA TGTGCAACAT CAACATCGAT GAGTGTGCCA GCAACCCATG CCACAATGGG 
GGCACATGCA AGGATGGCAT CAACGGCTTC ACCTGCCTCT GCCCCGAAGG CTTCCATGAC 
CCCAAGTGCC TGTCCGAAGT GAATGAGTGC AACAGCAACC CCTGCATCCA CGGGAGGTGC 
CACGACGGGC TGAACGGTTA CCGCTGTGAT TGCGACCCGG GCTGGAGCGG GACAAACTGT 
GACATCAACA ACAACGAGTG TGAATCCAAC CCCTGCATGA ATGGTGGCAC CTGCAAGGAC 
ATGACCAGCG GTTACATCTG CACCTGCAGG GAGGGGTTCA GCGGACCCAA CTGCCAGACC 
AATATCAATG AATGTGCTTC CAACCCCTGC CTGAACCAAG GCACGTGCAT CGATGATGTG 
GCCGGCTACA CCTGCAACTG CCTCCTGCCC TACACAGGAG CCACCTGTGA GGACGTGCTG 
GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC GGCGGCGAGT GCCGGGAGTC AGAGGACTAC 
AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC TGGCAAGGCC AGACGTGTGA GATTGACATC 
AATGAGTGTG TGAAGAGCCC GTGCCGCAAT GGGGCCACGT GTCAGAACAC CAACGGGAGC 
TACCGCTGCC TGTGCCGGGT GGGCTTCGCG GGCCGCAACT GCGACACCGA CATCGACGAC 
TGCCAGCCCA ATCCGTGCCA CAATGGTGGC TCCTGCTCGG ATGGCATTGG CACATTCTTC 
TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC AAGTGTGAGG AGGACATCAA TGAGTGTGCC 
AGCAACCCCT GCAAGAACGG GGCCAACTGC ACCGACTGCG TCAACAGCTA CACCTGCACC 
TGCCCCTCCG GCTTCAGCGG CATCCACTGC GAGAACAACA CACCAGACTG CACTGAGAGC 
TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT GGCATCAACA CCTTCACCTG CCTCTGTCCA 
TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC AACATCAACG AGTGTGACTC CAAGCCCTGC 
CTGAACGGGG GCACGTGTCA GGACAGCTAT GGGACGTACA AGTGCACCTG TCCCCAGGGA 
TACACTGGGC TCAACTGCCA GAACTTGGTG CGCTGGTGTG ACTCCTCACC CTGCAAAAAC 
GGGGGCAAGT GCTGGCAGAC CAACAACCTG TACCGCTGCG AGTGCAACAG CGGCTGGACG 
GGGCTCTACT GTGATGTCCC CAGCGTCTCC TGCGAGGTGG CTGCAAAGCA GCAAGGTATC 
GACGTCGCTC ATCTCTGCAG GAACTCAGGG CTCTGTGTGG ACAGTGGCAA CACTCACTTC 
TGCCGCTGCC AGGCTGGCTA CACCGGCAGC TACTGCGAGG AGCAGGTGGA TGAGTGCTCC 
CCCAACCCCT GCCAGAACGG AGCCACCTGC ACCGACTACC TGGGGGGCTA TTCCTGTGAG 
TGCGTGGCTG GTTATCATGG AGTTAACTGC TCAGAGGAGA TCAATGAGTG CTTGTCCCAC 
CCATGCCAGA ATGGAGGAAC CTGCATCGAT CTCATCAATA CCTACAAATG CTCCTGCCCC 
AGAGGAACTC AAGGGGTGCA CTGTGAGATC AATGTGGATG ACTGCAGCCC TTTCTTTGAT 
CCCGTCACCC TGGGGCCCAA GTGCTTTAAC AATGGCAAGT GCACGGATCG GGTAGGTGGC 
TACAGCTGCA TCTGCCCCCC TGGCTTTGTA GGGGAGCGCT GCGAGGGAGA CGTCAACGAG 
TGCCTCTCCA ACCCCTGCGA CGCGCGCGGC ACCCAGAACT GCGTGCAGCG GGTCAATGAC 
TACAAATGCG AGTGCCGGCC TGGCTACGCA GGGCGTCGCT GTGACACCGT GGTGGATGGC 
TGCAAGGGCA AACCCTGCAG GAATGGGGGA ACGTGCGCTG TGGCCAGCAA CACCGGCCGC 
GGCTTCATCT GCAAGTGCCC CCCGGGCTTT GTGGGTGCCA CCTGCGAGAA CGACTCCCAC 
ACCTGCGGGA CCCTGCACTG CCTGAACGGT GGCACCTGCA TCTCCATGCA CAAGAGCTCC 
AAGTGTGTGT GCGCAGCGGC CTTCACGGGC CCCGAGTGCC AATACCCGGC CAGCAGCCCC 
TGCATCTCCA ACCCCTGCTA CAACGGGGGC ACCTGCGAGT TCCTCAGCGA CGCCTCCCCT 
TACTACCACT GCAACTGCCC CGCCAACTTC AACGGCCTCA ACTGCCACAT CCTCGACTTT 
GATTTCCAAG GCGGCTTCGG GCAGGACATC ATCCCACCCA AAATCGAGGA GAAGTGTGAG 
ATCGCCGTTT GTGCGAGCTA TGCTGGCAAT AAGATCTGTG ATGGGAAGTG CAACAACCAC 
GCCTGCGGCT GGGACGGCGG CGACTGCTCG CTCAATTTCA ACGACCCCTG GAAGAACTGC 
TCACAGTCAC TGCAGTGCTG GAAGTACTTC AATGATGGCA AGTGTGACTC GCAGTGCAAC 
AACGCGGGCT GCCTGTATGA TGGGTTTGAC TGCCAGAAGT ACGAGGGGCA GTGCAACCCT 
CTGTACGACC AGTACTGTAA GGACCACTTC TCAGATGGTC ACTGTGACCA GGGCTGCAAT 
AACTTTGAGT GTGAGTGGGA CGGGCTGGAC TGTGCAAACA ACATGCCCGA GAAGCTGGCG 
GATGGCACGC TGGTGGTGGT GGTCCTCATC ACCCCTGAGA ACCTGAAGAA CAATTCCTTC 
AATTTCCTGA GGGAGCTGAG CCGTGTGCTG CACACCAATG TAGTCTTCAA GAAGAACGCC 
AAGGGAGAGT ACATGATCTT CCCATACTAT GGCAACGAGG AGGAGCTGAA AAAGCACTAC 
ATCAAGAGGT CAACGGAGGA CTGGGCAGAT ATGTCCAGTG CTGTCATCAA CAAGGTGAAG 
AGCAGCCTCT ACTCGCGGGC TGGCAGGAGG CAGAAGAGAG AGCTGGATCA GATGGACATC 
AGAGGATCCA TCGTCTACTT GGAAATTGAC AACAGGCAGT GCATCCAGTC GTCCTCCCAG 
TGCTTCCAGA GCGCAACCGA TGTGGCGGCC TTCCTGGGGG CCTTGGCCTC CCTGGGCAAC 
CTCAACATTC CCTACAAAAT AGAGGCCGTC AAGAGTGAAA CGGCGGAGCC CGCGAGGAAC 
TCCCAGCTGT ATCCTATGTA TGTGGTGGTG GCTGCACTGG TCTTGCTTGC CTTCATTGGA 
GTGGGAGTGC TGGTGTCCCG CAAGCGGCGC AGGGAGCATG GCCAGCTCTG GTTCCCAGAG 
GGCTTCAAAG TGACGGAGTC GAGCAAGAAG AAGCGCCGGG AACCACTTGG GGAAGATTCT 
GTTGGACTGA AACCCCTCAA AAATGCTTCT GACGGCACGC TGATGGACGA CAACCAAAAT 
GAGTGGGGTG ACGAGGAGAC CCTGGACACC AAGAAGTTCA GGTTCGAGGA GCAGGCGATG 
CTGCCCGACA CGGATGACCA GACGGACCAC AGGCAGTGGA CTCAGCAGCA CCTGGATGCT 
GCCGACCTCC GCATCTCCTC CATGGCCCCT ACCCCACCAC AGGGCGAGAT CGATGCAGAC 
TGCATGGATG TCAACGTACG AGGTCCAGAC GGCTTCACGC CCCTCATGAT CGCCTCGTGC 
AGCGGAGGGG GGCTGGAGAC TGGCAACAGC GAGGAGGAGG ACGATGCCCC TGCAGTCATC 
TCAGATTTCA TCTACCAAGG CGCCAGTTTG CACAACCAGA CTGACCGCAC CGGCGAGACC 
GCGCTGCACC TGGCTGCCCG CTACTCGCGC TCCGACGCTG CCAAACGCCT GCTGGAAGCC 
AGTGCTGATG CGAACATTCA GGATAACATG GGCAGGACCC CTCTGCACGC CGCTGTCTCT 
GCTGATGCCC AAGGAGTCTT CCAGATCCTG ATAAGGAACA GGGCGACTGA TCTCGATGCC 
CGAATGCACG ATGGGACGAC CCCACTGATC CTGGCTGCTC GCTTGGCTGT GGAGGGGATG 
CTGGAGGACC TCATCAACTG CCACGCAGAC GTCAACGCTG TGGATGATCT GGGCAAGTCA 
GCACTGCACT GGGCAGCTGC TGTGAACAAT GTCGAAGCCG CTGTCGTCCT CCTGAAGAAC 
GGTGCCAATA AGGATATGCA GAACAATAAG GAGGAGACCC CGCTGTTCCT CGCCGCCAGA 
GAAGGGAGCT ACGAAACAGC CAAGGTTCTG CTGGACCACT TTGCGAACCG CGACATCACG 
GACCACATGG ACCGGCTGCC GCGGGACATC GCGCAGGAGC GCATGCACCA CGACATCGTG 
CGGCTGCTGG ATGAGTACAA CCTGGTGCGG AGCCCTCCGC TGCACAGCGG CCCTCTCGGG 
GCCCCCACGC TGTCCCCCCC GCTCTGCTCC CCCAGCAGTT ACATCGGCAA CCTGAAGCCG 
GCCGTGCAGG GCAAGAAGGC CAGGAAGCCG AGCACCAAGG GCCTGAGCTG CAACGGCAAG 
GATTCCAAAG ACCTGAAAGC CCGGAGGAAA AAATCACAAG ATGGAAAAGG CTGTCTGCTC 
GACAACTCCA GCGTGCTGTC CCCAGTGGAC TCCCTGGAGT CACCCCACGG GTACCTGTCC 
GACGTCGCCT CCCCTCCGCT GATGACTTCT CCGTTCCAGC AGTCCCCTTC CATGCCTCTG 
AACCACCTGC CTGGCATGCC CGACGCCCAC ATGAGCATCA ACCACCTCAA CATGGCAGGG 
AAGCAGGAGA TGGCCCTGGG CGGCTCTGGC AGGATGGCCT TCGAGGCGGT GCCGCCGCGC 
CTCTCGCACC TCCCCGTCTC CAGCCCCAGC ACGGCGATGA GCAACGCCCC GATGAATTTC 
TCCGTCGGCG GAGCTGCCGG CCTGAGCGGG CAGTGCGACT GGCTGAGCCG GCTGCAGAGC 
GGCATGGTGC AGAACCAGTA CGGCGCCATG CGGGGCGGCA TGCAGCCGGG CACGCACCAG 
CAAGCACAGA ACCTGCAGCA CGGCATGATG AGCTCCCTGC ACAACGGGCT GCCCAGCACC 
AGCCTGTCGC AGATGATGAG CTACCAGGCC ATGCCCAGCA CCCGGCTGGC CTCCCAGCCC 
CACCTGCTGC AGAACCAGCA GATGCAGCAG ATGCAGCAGC CCGGAATGCA GCCGCAGCCC 
GGAATGCAGC CGCAGCCCGG CATGCAGCAG CCTCAACAGC AGCCCCAGCA ACAGCCCCAG 
CCGCAGCAGC ACCACAACCC CGGCTCCAAC GCCAGCGGCC ACATGGGCCA AAATTTCCTC 
GGTACTGAGC TGAGCCAGCC CGACATGCAG CCGGTGAGCA GCAGCGCCAT GGCGGTGCAC 
ACCATCCTGC CCCAAGATTC GCAGCTGCTA CCCACCTCTC TGCCGTCCTC CCTCGCGCAG 
CCCATGACCA CCACGCAGTT CCTGACCCCC CCTTCCCAGC ACAGCTATTC CTCCCCGTTG 
GACAACACCC CCAGCCACCA GCTCCAGGTG CCCGACCACC CCTTCCTCAC TCCCTCTCCG 
GAGTCTCCAG ACCAGTGGTC GAGCTCCTCG CCCCACTCCA ACGTGTCCGA CTGGTCCGAG 
GGCATCTCCA GCCCCCCCAC CAGCATGCAG TCGCAGATGG GACACATCCC CGAGGCCTTC 
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025466.1
CDS1..7686
Translation

Target ORF information:

RefSeq Version NM_001030295.1
Organism Gallus gallus(chicken)
Definition Gallus gallus notch 1 (NOTCH1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001030295.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
ATGGGGCGGT GTAGTGCTGC CCATCCCCGC GGTGGGGTGC ATTGCCCCGG GCTCTGTGCT 
GTGCCTGACG CTCTCCTTCT GTTCCCAGGT GTGCGGTGCA CGCAGCTGGC CGAATCCTGC 
CTCAACGGGG GCAAGTGTGA AACTTTCCTC AATGGGACGG AGGTGTGCCA GTGCAGCAGT 
GCCCATATGG GCGAGCGGTG CCAGCTGCCC AACCCCTGCC TCAGCTCCCC CTGCAAGAAC 
GCCGGCACCT GCATCCCCCT GCTGCGTGGC AGCACCGCCG ACTACACCTG TGTCTGCCGC 
CTGGGCTTCA CCGACGAGCT GTGCCTCACA CCCCTGGACA ACGCCTGCCT CAACAACCCC 
TGCCGCAACG GGGGCACCTG TGACCTGGTG ACACTCAGCG AGTACAAGTG CCGCTGCCCG 
CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG GCCGACCCCT GTGCCTCCAA CCCCTGTGCC 
AACGGGGGTC AGTGCGTCCC CTTCGAAGCC CACTACATCT GCCGCTGCAC GGCCGGCTTC 
CACGGGGCCA ACTGCAAGCA GGACGTGAAC GAATGCAACA TCTCACCACC AGTCTGCAAG 
AACGGTGGCA GCTGCACCAA CGAGGTGGGC ACCTACCAGT GCTCCTGCAA GCCAGCCTAC 
ACCGGGCAGA ACTGCGAGCA TCTCTATGTG CCCTGCAACC CCTCGCCCTG CCAGAATGGG 
GGCACGTGCC GCCAAACTGG GGACACCACC TACGACTGCA CCTGTCTGCC AGGGTTCACC 
GGGCAGAACT GCGAGGAGAA CATCGATGAC TGTCCAGGGA ACAACTGCAG GAATGGGGGC 
ACCTGCGTGG ATGGCGTCAA CACCTACAAC TGCCAGTGCC CGCCCGAGTG GACAGGTCAG 
TACTGCACCG AGGATGTGGA TGAGTGCCAG CTGATGCCCA ACGCCTGCCA GAACGGGGGC 
ACCTGCCACA ACAACCACGG CGGCTACAAC TGCGTCTGCG TCAATGGCTG GACGGGTGAG 
GACTGCAGTG AGAACATCGA TGACTGCGCC ATGGCTGCCT GCTTCCAGGG GGCCACCTGC 
CATGACCGGG TGGCCTCCTT CTACTGCGAG TGTCCCCATG GGCGCACAGG TTTGCTGTGC 
CACCTGGATG ACGCCTGCAT CAGCAACCCC TGCAACGAGG GCTCCAACTG CGATACCAAC 
CCTGTCAATG GCAAAGCCAT CTGCACGTGT CCTTCGGGGT ACATGGGGCC GGCGTGCAAC 
CAGGACGTGG ATGAGTGCTC ACTGGGAGCC AACCCCTGTG AGCACGCAGG GAAATGCATC 
AACACCCAGG GGTCCTTCCA ATGCCAGTGT CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG 
ATCGATGTCA ATGAGTGCCT CTCCAACCCC TGCCAGAACG ATGCCACCTG CCTGGACCAG 
ATCGGGGAGT TCCAGTGCAT CTGCATGCCT GGATACGAGG GCGTCTACTG TGAAATCAAC 
ACAGATGAGT GTGCCAGCAG CCCCTGCCTG CACAATGGCA ACTGCCTGGA TAAGATCAAC 
GAGTTCCACT GCGAGTGCCC CACTGGGTTC AACGGGCACC TGTGCCAGTT TGACATCGAT 
GAGTGTGCCA GCACCCCCTG CAAGAATGGG GCCAAGTGCG TGGATGGCCC CAACACCTAC 
AGCTGCGAGT GCACCGAAGG TTTCTCAGGT GTTCACTGTG AGATCGACAT CGATGAGTGC 
AACCCCGACC CGTGCCACTA TGGGACCTGC AAGGACAGCA TCGCCGCCTT CACCTGCCTC 
TGCCAGCCCG GCTACACGGG CCACCGCTGC GACATCAACA TCAATGAGTG CCAGAGCCAG 
CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC AGGGACAACG CCTACAACTG TCTGTGCCTC 
AAGGGGACCA CGGGGCCCAA CTGCGAGATC AACCTGGACG ACTGCGCCAG CAACCCCTGC 
GACTACGGCA AGTGCATCGA CAAGATCAAC GGCTATGAGT GCACCTGTGA GCCGGGGTAC 
ACAGGGCGCA TGTGCAACAT CAACATCGAT GAGTGTGCCA GCAACCCATG CCACAATGGG 
GGCACATGCA AGGATGGCAT CAACGGCTTC ACCTGCCTCT GCCCCGAAGG CTTCCATGAC 
CCCAAGTGCC TGTCCGAAGT GAATGAGTGC AACAGCAACC CCTGCATCCA CGGGAGGTGC 
CACGACGGGC TGAACGGTTA CCGCTGTGAT TGCGACCCGG GCTGGAGCGG GACAAACTGT 
GACATCAACA ACAACGAGTG TGAATCCAAC CCCTGCATGA ATGGTGGCAC CTGCAAGGAC 
ATGACCAGCG GTTACATCTG CACCTGCAGG GAGGGGTTCA GCGGACCCAA CTGCCAGACC 
AATATCAATG AATGTGCTTC CAACCCCTGC CTGAACCAAG GCACGTGCAT CGATGATGTG 
GCCGGCTACA CCTGCAACTG CCTCCTGCCC TACACAGGAG CCACCTGTGA GGACGTGCTG 
GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC GGCGGCGAGT GCCGGGAGTC AGAGGACTAC 
AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC TGGCAAGGCC AGACGTGTGA GATTGACATC 
AATGAGTGTG TGAAGAGCCC GTGCCGCAAT GGGGCCACGT GTCAGAACAC CAACGGGAGC 
TACCGCTGCC TGTGCCGGGT GGGCTTCGCG GGCCGCAACT GCGACACCGA CATCGACGAC 
TGCCAGCCCA ATCCGTGCCA CAATGGTGGC TCCTGCTCGG ATGGCATTGG CACATTCTTC 
TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC AAGTGTGAGG AGGACATCAA TGAGTGTGCC 
AGCAACCCCT GCAAGAACGG GGCCAACTGC ACCGACTGCG TCAACAGCTA CACCTGCACC 
TGCCCCTCCG GCTTCAGCGG CATCCACTGC GAGAACAACA CACCAGACTG CACTGAGAGC 
TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT GGCATCAACA CCTTCACCTG CCTCTGTCCA 
TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC AACATCAACG AGTGTGACTC CAAGCCCTGC 
CTGAACGGGG GCACGTGTCA GGACAGCTAT GGGACGTACA AGTGCACCTG TCCCCAGGGA 
TACACTGGGC TCAACTGCCA GAACTTGGTG CGCTGGTGTG ACTCCTCACC CTGCAAAAAC 
GGGGGCAAGT GCTGGCAGAC CAACAACCTG TACCGCTGCG AGTGCAACAG CGGCTGGACG 
GGGCTCTACT GTGATGTCCC CAGCGTCTCC TGCGAGGTGG CTGCAAAGCA GCAAGGTATC 
GACGTCGCTC ATCTCTGCAG GAACTCAGGG CTCTGTGTGG ACAGTGGCAA CACTCACTTC 
TGCCGCTGCC AGGCTGGCTA CACCGGCAGC TACTGCGAGG AGCAGGTGGA TGAGTGCTCC 
CCCAACCCCT GCCAGAACGG AGCCACCTGC ACCGACTACC TGGGGGGCTA TTCCTGTGAG 
TGCGTGGCTG GTTATCATGG AGTTAACTGC TCAGAGGAGA TCAATGAGTG CTTGTCCCAC 
CCATGCCAGA ATGGAGGAAC CTGCATCGAT CTCATCAATA CCTACAAATG CTCCTGCCCC 
AGAGGAACTC AAGGGGTGCA CTGTGAGATC AATGTGGATG ACTGCAGCCC TTTCTTTGAT 
CCCGTCACCC TGGGGCCCAA GTGCTTTAAC AATGGCAAGT GCACGGATCG GGTAGGTGGC 
TACAGCTGCA TCTGCCCCCC TGGCTTTGTA GGGGAGCGCT GCGAGGGAGA CGTCAACGAG 
TGCCTCTCCA ACCCCTGCGA CGCGCGCGGC ACCCAGAACT GCGTGCAGCG GGTCAATGAC 
TACAAATGCG AGTGCCGGCC TGGCTACGCA GGGCGTCGCT GTGACACCGT GGTGGATGGC 
TGCAAGGGCA AACCCTGCAG GAATGGGGGA ACGTGCGCTG TGGCCAGCAA CACCGGCCGC 
GGCTTCATCT GCAAGTGCCC CCCGGGCTTT GTGGGTGCCA CCTGCGAGAA CGACTCCCAC 
ACCTGCGGGA CCCTGCACTG CCTGAACGGT GGCACCTGCA TCTCCATGCA CAAGAGCTCC 
AAGTGTGTGT GCGCAGCGGC CTTCACGGGC CCCGAGTGCC AATACCCGGC CAGCAGCCCC 
TGCATCTCCA ACCCCTGCTA CAACGGGGGC ACCTGCGAGT TCCTCAGCGA CGCCTCCCCT 
TACTACCACT GCAACTGCCC CGCCAACTTC AACGGCCTCA ACTGCCACAT CCTCGACTTT 
GATTTCCAAG GCGGCTTCGG GCAGGACATC ATCCCACCCA AAATCGAGGA GAAGTGTGAG 
ATCGCCGTTT GTGCGAGCTA TGCTGGCAAT AAGATCTGTG ATGGGAAGTG CAACAACCAC 
GCCTGCGGCT GGGACGGCGG CGACTGCTCG CTCAATTTCA ACGACCCCTG GAAGAACTGC 
TCACAGTCAC TGCAGTGCTG GAAGTACTTC AATGATGGCA AGTGTGACTC GCAGTGCAAC 
AACGCGGGCT GCCTGTATGA TGGGTTTGAC TGCCAGAAGT ACGAGGGGCA GTGCAACCCT 
CTGTACGACC AGTACTGTAA GGACCACTTC TCAGATGGTC ACTGTGACCA GGGCTGCAAT 
AACTTTGAGT GTGAGTGGGA CGGGCTGGAC TGTGCAAACA ACATGCCCGA GAAGCTGGCG 
GATGGCACGC TGGTGGTGGT GGTCCTCATC ACCCCTGAGA ACCTGAAGAA CAATTCCTTC 
AATTTCCTGA GGGAGCTGAG CCGTGTGCTG CACACCAATG TAGTCTTCAA GAAGAACGCC 
AAGGGAGAGT ACATGATCTT CCCATACTAT GGCAACGAGG AGGAGCTGAA AAAGCACTAC 
ATCAAGAGGT CAACGGAGGA CTGGGCAGAT ATGTCCAGTG CTGTCATCAA CAAGGTGAAG 
AGCAGCCTCT ACTCGCGGGC TGGCAGGAGG CAGAAGAGAG AGCTGGATCA GATGGACATC 
AGAGGATCCA TCGTCTACTT GGAAATTGAC AACAGGCAGT GCATCCAGTC GTCCTCCCAG 
TGCTTCCAGA GCGCAACCGA TGTGGCGGCC TTCCTGGGGG CCTTGGCCTC CCTGGGCAAC 
CTCAACATTC CCTACAAAAT AGAGGCCGTC AAGAGTGAAA CGGCGGAGCC CGCGAGGAAC 
TCCCAGCTGT ATCCTATGTA TGTGGTGGTG GCTGCACTGG TCTTGCTTGC CTTCATTGGA 
GTGGGAGTGC TGGTGTCCCG CAAGCGGCGC AGGGAGCATG GCCAGCTCTG GTTCCCAGAG 
GGCTTCAAAG TGACGGAGTC GAGCAAGAAG AAGCGCCGGG AACCACTTGG GGAAGATTCT 
GTTGGACTGA AACCCCTCAA AAATGCTTCT GACGGCACGC TGATGGACGA CAACCAAAAT 
GAGTGGGGTG ACGAGGAGAC CCTGGACACC AAGAAGTTCA GGTTCGAGGA GCAGGCGATG 
CTGCCCGACA CGGATGACCA GACGGACCAC AGGCAGTGGA CTCAGCAGCA CCTGGATGCT 
GCCGACCTCC GCATCTCCTC CATGGCCCCT ACCCCACCAC AGGGCGAGAT CGATGCAGAC 
TGCATGGATG TCAACGTACG AGGTCCAGAC GGCTTCACGC CCCTCATGAT CGCCTCGTGC 
AGCGGAGGGG GGCTGGAGAC TGGCAACAGC GAGGAGGAGG ACGATGCCCC TGCAGTCATC 
TCAGATTTCA TCTACCAAGG CGCCAGTTTG CACAACCAGA CTGACCGCAC CGGCGAGACC 
GCGCTGCACC TGGCTGCCCG CTACTCGCGC TCCGACGCTG CCAAACGCCT GCTGGAAGCC 
AGTGCTGATG CGAACATTCA GGATAACATG GGCAGGACCC CTCTGCACGC CGCTGTCTCT 
GCTGATGCCC AAGGAGTCTT CCAGATCCTG ATAAGGAACA GGGCGACTGA TCTCGATGCC 
CGAATGCACG ATGGGACGAC CCCACTGATC CTGGCTGCTC GCTTGGCTGT GGAGGGGATG 
CTGGAGGACC TCATCAACTG CCACGCAGAC GTCAACGCTG TGGATGATCT GGGCAAGTCA 
GCACTGCACT GGGCAGCTGC TGTGAACAAT GTCGAAGCCG CTGTCGTCCT CCTGAAGAAC 
GGTGCCAATA AGGATATGCA GAACAATAAG GAGGAGACCC CGCTGTTCCT CGCCGCCAGA 
GAAGGGAGCT ACGAAACAGC CAAGGTTCTG CTGGACCACT TTGCGAACCG CGACATCACG 
GACCACATGG ACCGGCTGCC GCGGGACATC GCGCAGGAGC GCATGCACCA CGACATCGTG 
CGGCTGCTGG ATGAGTACAA CCTGGTGCGG AGCCCTCCGC TGCACAGCGG CCCTCTCGGG 
GCCCCCACGC TGTCCCCCCC GCTCTGCTCC CCCAGCAGTT ACATCGGCAA CCTGAAGCCG 
GCCGTGCAGG GCAAGAAGGC CAGGAAGCCG AGCACCAAGG GCCTGAGCTG CAACGGCAAG 
GATTCCAAAG ACCTGAAAGC CCGGAGGAAA AAATCACAAG ATGGAAAAGG CTGTCTGCTC 
GACAACTCCA GCGTGCTGTC CCCAGTGGAC TCCCTGGAGT CACCCCACGG GTACCTGTCC 
GACGTCGCCT CCCCTCCGCT GATGACTTCT CCGTTCCAGC AGTCCCCTTC CATGCCTCTG 
AACCACCTGC CTGGCATGCC CGACGCCCAC ATGAGCATCA ACCACCTCAA CATGGCAGGG 
AAGCAGGAGA TGGCCCTGGG CGGCTCTGGC AGGATGGCCT TCGAGGCGGT GCCGCCGCGC 
CTCTCGCACC TCCCCGTCTC CAGCCCCAGC ACGGCGATGA GCAACGCCCC GATGAATTTC 
TCCGTCGGCG GAGCTGCCGG CCTGAGCGGG CAGTGCGACT GGCTGAGCCG GCTGCAGAGC 
GGCATGGTGC AGAACCAGTA CGGCGCCATG CGGGGCGGCA TGCAGCCGGG CACGCACCAG 
CAAGCACAGA ACCTGCAGCA CGGCATGATG AGCTCCCTGC ACAACGGGCT GCCCAGCACC 
AGCCTGTCGC AGATGATGAG CTACCAGGCC ATGCCCAGCA CCCGGCTGGC CTCCCAGCCC 
CACCTGCTGC AGAACCAGCA GATGCAGCAG ATGCAGCAGC CCGGAATGCA GCCGCAGCCC 
GGAATGCAGC CGCAGCCCGG CATGCAGCAG CCTCAACAGC AGCCCCAGCA ACAGCCCCAG 
CCGCAGCAGC ACCACAACCC CGGCTCCAAC GCCAGCGGCC ACATGGGCCA AAATTTCCTC 
GGTACTGAGC TGAGCCAGCC CGACATGCAG CCGGTGAGCA GCAGCGCCAT GGCGGTGCAC 
ACCATCCTGC CCCAAGATTC GCAGCTGCTA CCCACCTCTC TGCCGTCCTC CCTCGCGCAG 
CCCATGACCA CCACGCAGTT CCTGACCCCC CCTTCCCAGC ACAGCTATTC CTCCCCGTTG 
GACAACACCC CCAGCCACCA GCTCCAGGTG CCCGACCACC CCTTCCTCAC TCCCTCTCCG 
GAGTCTCCAG ACCAGTGGTC GAGCTCCTCG CCCCACTCCA ACGTGTCCGA CTGGTCCGAG 
GGCATCTCCA GCCCCCCCAC CAGCATGCAG TCGCAGATGG GACACATCCC CGAGGCCTTC 
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa43346
Clone ID Related Accession (Same CDS sequence) XM_015279324.1 , XM_015279324.2
Accession Version XM_015279324.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 7656bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product neurogenic locus notch homolog protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGTGCA CTGTGAGATC 
AATGTGGATG ACTGCAGCCC TTTCTTTGAT CCCGTCACCC TGGGGCCCAA GTGCTTTAAC 
AATGGCAAGT GCACGGATCG GGTAGGTGGC TACAGCTGCA TCTGCCCCCC TGGCTTTGTA 
GGGGAGCGCT GCGAGGGAGA CGTCAACGAG TGCCTCTCCA ACCCCTGCGA CGCGCGCGGC 
ACCCAGAACT GCGTGCAGCG GGTCAATGAC TACAAATGCG AGTGCCGGCC TGGCTACGCA 
GGGCGTCGCT GTGACACCGT GGTGGATGGC TGCAAGGGCA AACCCTGCAG GAATGGGGGA 
ACGTGCGCTG TGGCCAGCAA CACCGGCCGC GGCTTCATCT GCAAGTGCCC CCCGGGCTTT 
GTGGGTGCCA CCTGCGAGAA CGACTCCCAC ACCTGCGGGA CCCTGCACTG CCTGAACGGT 
GGCACCTGCA TCTCCATGCA CAAGAGCTCC AAGTGTGTGT GCGCAGCGGC CTTCACGGGC 
CCCGAGTGCC AATACCCGGC CAGCAGCCCC TGCATCTCCA ACCCCTGCTA CAACGGGGGC 
ACCTGCGAGT TCCTCAGCGA CGCCTCCCCT TACTACCACT GCAACTGCCC CGCCAACTTC 
AACGGCCTCA ACTGCCACAT CCTCGACTTT GATTTCCAAG GCGGCTTCGG GCAGGACATC 
ATCCCACCCA AAATCGAGGA GAAGTGTGAG ATCGCCGTTT GTGCGAGCTA TGCTGGCAAT 
AAGATCTGTG ATGGGAAGTG CAACAACCAC GCCTGCGGCT GGGACGGCGG CGACTGCTCG 
CTCAATTTCA ACGACCCCTG GAAGAACTGC TCACAGTCAC TGCAGTGCTG GAAGTACTTC 
AATGATGGCA AGTGTGACTC GCAGTGCAAC AACGCGGGCT GCCTGTATGA TGGGTTTGAC 
TGCCAGAAGT ACGAGGGGCA GTGCAACCCT CTGTACGACC AGTACTGTAA GGACCACTTC 
TCAGATGGTC ACTGTGACCA GGGCTGCAAT AACTTTGAGT GTGAGTGGGA CGGGCTGGAC 
TGTGCAAACA ACATGCCCGA GAAGCTGGCG GATGGCACGC TGGTGGTGGT GGTCCTCATC 
ACCCCTGAGA ACCTGAAGAA CAATTCCTTC AATTTCCTGA GGGAGCTGAG CCGTGTGCTG 
CACACCAATG TAGTCTTCAA GAAGAACGCC AAGGGAGAGT ACATGATCTT CCCATACTAT 
GGCAACGAGG AGGAGCTGAA AAAGCACTAC ATCAAGAGGT CAACGGAGGA CTGGGCAGAT 
ATGTCCAGTG CTGTCATCAA CAAGGTGAAG AGCAGCCTCT ACTCGCGGGC TGGCAGGAGG 
CAGAAGAGAG AGCTGGATCA GATGGACATC AGAGGATCCA TCGTCTACTT GGAAATTGAC 
AACAGGCAGT GCATCCAGTC GTCCTCCCAG TGCTTCCAGA GCGCAACCGA TGTGGCGGCC 
TTCCTGGGGG CCTTGGCCTC CCTGGGCAAC CTCAACATTC CCTACAAAAT AGAGGCCGTC 
AAGAGTGAAA CGGCGGAGCC CGCGAGGAAC TCCCAGCTGT ATCCTATGTA TGTGGTGGTG 
GCTGCACTGG TCTTGCTTGC CTTCATTGGA GTGGGAGTGC TGGTGTCCCG CAAGCGGCGC 
AGGGAGCATG GCCAGCTCTG GTTCCCAGAG GGCTTCAAAG TGACGGAGTC GAGCAAGAAG 
AAGCGCCGGG AACCACTTGG GGAAGATTCT GTTGGACTGA AACCCCTCAA AAATGCTTCT 
GACGGCACGC TGATGGACGA CAACCAAAAT GAGTGGGGTG ACGAGGAGAC CCTGGACACC 
AAGAAGTTCA GGTTCGAGGA GCAGGCGATG CTGCCCGACA CGGATGACCA GACGGACCAC 
AGGCAGTGGA CTCAGCAGCA CCTGGATGCT GCCGACCTCC GCATCTCCTC CATGGCCCCT 
ACCCCACCAC AGGGCGAGAT CGATGCAGAC TGCATGGATG TCAACGTACG AGGTCCAGAC 
GGCTTCACGC CCCTCATGAT CGCCTCGTGC AGCGGAGGGG GGCTGGAGAC TGGCAACAGC 
GAGGAGGAGG ACGATGCCCC TGCAGTCATC TCAGATTTCA TCTACCAAGG CGCCAGTTTG 
CACAACCAGA CTGACCGCAC CGGCGAGACC GCGCTGCACC TGGCTGCCCG CTACTCGCGC 
TCCGACGCTG CCAAACGCCT GCTGGAAGCC AGTGCTGATG CGAACATTCA GGATAACATG 
GGCAGGACCC CTCTGCACGC CGCTGTCTCT GCTGATGCCC AAGGAGTCTT CCAGATCCTG 
ATAAGGAACA GGGCGACTGA TCTCGATGCC CGAATGCACG ATGGGACGAC CCCACTGATC 
CTGGCTGCTC GCTTGGCTGT GGAGGGGATG CTGGAGGACC TCATCAACTG CCACGCAGAC 
GTCAACGCTG TGGATGATCT GGGCAAGTCA GCACTGCACT GGGCAGCTGC TGTGAACAAT 
GTCGAAGCCG CTGTCGTCCT CCTGAAGAAC GGTGCCAATA AGGATATGCA GAACAATAAG 
GAGGAGACCC CGCTGTTCCT CGCCGCCAGA GAAGGGAGCT ACGAAACAGC CAAGGTTCTG 
CTGGACCACT TTGCGAACCG CGACATCACG GACCACATGG ACCGGCTGCC GCGGGACATC 
GCGCAGGAGC GCATGCACCA CGACATCGTG CGGCTGCTGG ATGAGTACAA CCTGGTGCGG 
AGCCCTCCGC TGCACAGCGG CCCTCTCGGG GCCCCCACGC TGTCCCCCCC GCTCTGCTCC 
CCCAGCAGTT ACATCGGCAA CCTGAAGCCG GCCGTGCAGG GCAAGAAGGC CAGGAAGCCG 
AGCACCAAGG GCCTGAGCTG CAACGGCAAG GATTCCAAAG ACCTGAAAGC CCGGAGGAAA 
AAATCACAAG ATGGAAAAGG CTGTCTGCTC GACAACTCCA GCGTGCTGTC CCCAGTGGAC 
TCCCTGGAGT CACCCCACGG GTACCTGTCC GACGTCGCCT CCCCTCCGCT GATGACTTCT 
CCGTTCCAGC AGTCCCCTTC CATGCCTCTG AACCACCTGC CTGGCATGCC CGACGCCCAC 
ATGAGCATCA ACCACCTCAA CATGGCAGGG AAGCAGGAGA TGGCCCTGGG CGGCTCTGGC 
AGGATGGCCT TCGAGGCGGT GCCGCCGCGC CTCTCGCACC TCCCCGTCTC CAGCCCCAGC 
ACGGCGATGA GCAACGCCCC GATGAATTTC TCCGTCGGCG GAGCTGCCGG CCTGAGCGGG 
CAGTGCGACT GGCTGAGCCG GCTGCAGAGC GGCATGGTGC AGAACCAGTA CGGCGCCATG 
CGGGGCGGCA TGCAGCCGGG CACGCACCAG CAAGCACAGA ACCTGCAGCA CGGCATGATG 
AGCTCCCTGC ACAACGGGCT GCCCAGCACC AGCCTGTCGC AGATGATGAG CTACCAGGCC 
ATGCCCAGCA CCCGGCTGGC CTCCCAGCCC CACCTGCTGC AGAACCAGCA GATGCAGCAG 
ATGCAGCAGC CCGGAATGCA GCCGCAGCCC GGAATGCAGC CGCAGCCCGG CATGCAGCAG 
CCTCAACAGC AGCCCCAGCA ACAGCCCCAG CCGCAGCAGC ACCACAACCC CGGCTCCAAC 
GCCAGCGGCC ACATGGGCCA AAATTTCCTC GGTACTGAGC TGAGCCAGCC CGACATGCAG 
CCGGTGAGCA GCAGCGCCAT GGCGGTGCAC ACCATCCTGC CCCAAGATTC GCAGCTGCTA 
CCCACCTCTC TGCCGTCCTC CCTCGCGCAG CCCATGACCA CCACGCAGTT CCTGACCCCC 
CCTTCCCAGC ACAGCTATTC CTCCCCGTTG GACAACACCC CCAGCCACCA GCTCCAGGTG 
CCCGACCACC CCTTCCTCAC TCCCTCTCCG GAGTCTCCAG ACCAGTGGTC GAGCTCCTCG 
CCCCACTCCA ACGTGTCCGA CTGGTCCGAG GGCATCTCCA GCCCCCCCAC CAGCATGCAG 
TCGCAGATGG GACACATCCC CGAGGCCTTC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015134810.1
CDS189..7844
Translation

Target ORF information:

RefSeq Version XM_015279324.1
Organism Gallus gallus(chicken)
Definition Gallus gallus notch 1 (NOTCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015279324.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGTGCA CTGTGAGATC 
AATGTGGATG ACTGCAGCCC TTTCTTTGAT CCCGTCACCC TGGGGCCCAA GTGCTTTAAC 
AATGGCAAGT GCACGGATCG GGTAGGTGGC TACAGCTGCA TCTGCCCCCC TGGCTTTGTA 
GGGGAGCGCT GCGAGGGAGA CGTCAACGAG TGCCTCTCCA ACCCCTGCGA CGCGCGCGGC 
ACCCAGAACT GCGTGCAGCG GGTCAATGAC TACAAATGCG AGTGCCGGCC TGGCTACGCA 
GGGCGTCGCT GTGACACCGT GGTGGATGGC TGCAAGGGCA AACCCTGCAG GAATGGGGGA 
ACGTGCGCTG TGGCCAGCAA CACCGGCCGC GGCTTCATCT GCAAGTGCCC CCCGGGCTTT 
GTGGGTGCCA CCTGCGAGAA CGACTCCCAC ACCTGCGGGA CCCTGCACTG CCTGAACGGT 
GGCACCTGCA TCTCCATGCA CAAGAGCTCC AAGTGTGTGT GCGCAGCGGC CTTCACGGGC 
CCCGAGTGCC AATACCCGGC CAGCAGCCCC TGCATCTCCA ACCCCTGCTA CAACGGGGGC 
ACCTGCGAGT TCCTCAGCGA CGCCTCCCCT TACTACCACT GCAACTGCCC CGCCAACTTC 
AACGGCCTCA ACTGCCACAT CCTCGACTTT GATTTCCAAG GCGGCTTCGG GCAGGACATC 
ATCCCACCCA AAATCGAGGA GAAGTGTGAG ATCGCCGTTT GTGCGAGCTA TGCTGGCAAT 
AAGATCTGTG ATGGGAAGTG CAACAACCAC GCCTGCGGCT GGGACGGCGG CGACTGCTCG 
CTCAATTTCA ACGACCCCTG GAAGAACTGC TCACAGTCAC TGCAGTGCTG GAAGTACTTC 
AATGATGGCA AGTGTGACTC GCAGTGCAAC AACGCGGGCT GCCTGTATGA TGGGTTTGAC 
TGCCAGAAGT ACGAGGGGCA GTGCAACCCT CTGTACGACC AGTACTGTAA GGACCACTTC 
TCAGATGGTC ACTGTGACCA GGGCTGCAAT AACTTTGAGT GTGAGTGGGA CGGGCTGGAC 
TGTGCAAACA ACATGCCCGA GAAGCTGGCG GATGGCACGC TGGTGGTGGT GGTCCTCATC 
ACCCCTGAGA ACCTGAAGAA CAATTCCTTC AATTTCCTGA GGGAGCTGAG CCGTGTGCTG 
CACACCAATG TAGTCTTCAA GAAGAACGCC AAGGGAGAGT ACATGATCTT CCCATACTAT 
GGCAACGAGG AGGAGCTGAA AAAGCACTAC ATCAAGAGGT CAACGGAGGA CTGGGCAGAT 
ATGTCCAGTG CTGTCATCAA CAAGGTGAAG AGCAGCCTCT ACTCGCGGGC TGGCAGGAGG 
CAGAAGAGAG AGCTGGATCA GATGGACATC AGAGGATCCA TCGTCTACTT GGAAATTGAC 
AACAGGCAGT GCATCCAGTC GTCCTCCCAG TGCTTCCAGA GCGCAACCGA TGTGGCGGCC 
TTCCTGGGGG CCTTGGCCTC CCTGGGCAAC CTCAACATTC CCTACAAAAT AGAGGCCGTC 
AAGAGTGAAA CGGCGGAGCC CGCGAGGAAC TCCCAGCTGT ATCCTATGTA TGTGGTGGTG 
GCTGCACTGG TCTTGCTTGC CTTCATTGGA GTGGGAGTGC TGGTGTCCCG CAAGCGGCGC 
AGGGAGCATG GCCAGCTCTG GTTCCCAGAG GGCTTCAAAG TGACGGAGTC GAGCAAGAAG 
AAGCGCCGGG AACCACTTGG GGAAGATTCT GTTGGACTGA AACCCCTCAA AAATGCTTCT 
GACGGCACGC TGATGGACGA CAACCAAAAT GAGTGGGGTG ACGAGGAGAC CCTGGACACC 
AAGAAGTTCA GGTTCGAGGA GCAGGCGATG CTGCCCGACA CGGATGACCA GACGGACCAC 
AGGCAGTGGA CTCAGCAGCA CCTGGATGCT GCCGACCTCC GCATCTCCTC CATGGCCCCT 
ACCCCACCAC AGGGCGAGAT CGATGCAGAC TGCATGGATG TCAACGTACG AGGTCCAGAC 
GGCTTCACGC CCCTCATGAT CGCCTCGTGC AGCGGAGGGG GGCTGGAGAC TGGCAACAGC 
GAGGAGGAGG ACGATGCCCC TGCAGTCATC TCAGATTTCA TCTACCAAGG CGCCAGTTTG 
CACAACCAGA CTGACCGCAC CGGCGAGACC GCGCTGCACC TGGCTGCCCG CTACTCGCGC 
TCCGACGCTG CCAAACGCCT GCTGGAAGCC AGTGCTGATG CGAACATTCA GGATAACATG 
GGCAGGACCC CTCTGCACGC CGCTGTCTCT GCTGATGCCC AAGGAGTCTT CCAGATCCTG 
ATAAGGAACA GGGCGACTGA TCTCGATGCC CGAATGCACG ATGGGACGAC CCCACTGATC 
CTGGCTGCTC GCTTGGCTGT GGAGGGGATG CTGGAGGACC TCATCAACTG CCACGCAGAC 
GTCAACGCTG TGGATGATCT GGGCAAGTCA GCACTGCACT GGGCAGCTGC TGTGAACAAT 
GTCGAAGCCG CTGTCGTCCT CCTGAAGAAC GGTGCCAATA AGGATATGCA GAACAATAAG 
GAGGAGACCC CGCTGTTCCT CGCCGCCAGA GAAGGGAGCT ACGAAACAGC CAAGGTTCTG 
CTGGACCACT TTGCGAACCG CGACATCACG GACCACATGG ACCGGCTGCC GCGGGACATC 
GCGCAGGAGC GCATGCACCA CGACATCGTG CGGCTGCTGG ATGAGTACAA CCTGGTGCGG 
AGCCCTCCGC TGCACAGCGG CCCTCTCGGG GCCCCCACGC TGTCCCCCCC GCTCTGCTCC 
CCCAGCAGTT ACATCGGCAA CCTGAAGCCG GCCGTGCAGG GCAAGAAGGC CAGGAAGCCG 
AGCACCAAGG GCCTGAGCTG CAACGGCAAG GATTCCAAAG ACCTGAAAGC CCGGAGGAAA 
AAATCACAAG ATGGAAAAGG CTGTCTGCTC GACAACTCCA GCGTGCTGTC CCCAGTGGAC 
TCCCTGGAGT CACCCCACGG GTACCTGTCC GACGTCGCCT CCCCTCCGCT GATGACTTCT 
CCGTTCCAGC AGTCCCCTTC CATGCCTCTG AACCACCTGC CTGGCATGCC CGACGCCCAC 
ATGAGCATCA ACCACCTCAA CATGGCAGGG AAGCAGGAGA TGGCCCTGGG CGGCTCTGGC 
AGGATGGCCT TCGAGGCGGT GCCGCCGCGC CTCTCGCACC TCCCCGTCTC CAGCCCCAGC 
ACGGCGATGA GCAACGCCCC GATGAATTTC TCCGTCGGCG GAGCTGCCGG CCTGAGCGGG 
CAGTGCGACT GGCTGAGCCG GCTGCAGAGC GGCATGGTGC AGAACCAGTA CGGCGCCATG 
CGGGGCGGCA TGCAGCCGGG CACGCACCAG CAAGCACAGA ACCTGCAGCA CGGCATGATG 
AGCTCCCTGC ACAACGGGCT GCCCAGCACC AGCCTGTCGC AGATGATGAG CTACCAGGCC 
ATGCCCAGCA CCCGGCTGGC CTCCCAGCCC CACCTGCTGC AGAACCAGCA GATGCAGCAG 
ATGCAGCAGC CCGGAATGCA GCCGCAGCCC GGAATGCAGC CGCAGCCCGG CATGCAGCAG 
CCTCAACAGC AGCCCCAGCA ACAGCCCCAG CCGCAGCAGC ACCACAACCC CGGCTCCAAC 
GCCAGCGGCC ACATGGGCCA AAATTTCCTC GGTACTGAGC TGAGCCAGCC CGACATGCAG 
CCGGTGAGCA GCAGCGCCAT GGCGGTGCAC ACCATCCTGC CCCAAGATTC GCAGCTGCTA 
CCCACCTCTC TGCCGTCCTC CCTCGCGCAG CCCATGACCA CCACGCAGTT CCTGACCCCC 
CCTTCCCAGC ACAGCTATTC CTCCCCGTTG GACAACACCC CCAGCCACCA GCTCCAGGTG 
CCCGACCACC CCTTCCTCAC TCCCTCTCCG GAGTCTCCAG ACCAGTGGTC GAGCTCCTCG 
CCCCACTCCA ACGTGTCCGA CTGGTCCGAG GGCATCTCCA GCCCCCCCAC CAGCATGCAG 
TCGCAGATGG GACACATCCC CGAGGCCTTC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa43346
Clone ID Related Accession (Same CDS sequence) XM_015279324.1 , XM_015279324.2
Accession Version XM_015279324.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 7656bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product neurogenic locus notch homolog protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGTGCA CTGTGAGATC 
AATGTGGATG ACTGCAGCCC TTTCTTTGAT CCCGTCACCC TGGGGCCCAA GTGCTTTAAC 
AATGGCAAGT GCACGGATCG GGTAGGTGGC TACAGCTGCA TCTGCCCCCC TGGCTTTGTA 
GGGGAGCGCT GCGAGGGAGA CGTCAACGAG TGCCTCTCCA ACCCCTGCGA CGCGCGCGGC 
ACCCAGAACT GCGTGCAGCG GGTCAATGAC TACAAATGCG AGTGCCGGCC TGGCTACGCA 
GGGCGTCGCT GTGACACCGT GGTGGATGGC TGCAAGGGCA AACCCTGCAG GAATGGGGGA 
ACGTGCGCTG TGGCCAGCAA CACCGGCCGC GGCTTCATCT GCAAGTGCCC CCCGGGCTTT 
GTGGGTGCCA CCTGCGAGAA CGACTCCCAC ACCTGCGGGA CCCTGCACTG CCTGAACGGT 
GGCACCTGCA TCTCCATGCA CAAGAGCTCC AAGTGTGTGT GCGCAGCGGC CTTCACGGGC 
CCCGAGTGCC AATACCCGGC CAGCAGCCCC TGCATCTCCA ACCCCTGCTA CAACGGGGGC 
ACCTGCGAGT TCCTCAGCGA CGCCTCCCCT TACTACCACT GCAACTGCCC CGCCAACTTC 
AACGGCCTCA ACTGCCACAT CCTCGACTTT GATTTCCAAG GCGGCTTCGG GCAGGACATC 
ATCCCACCCA AAATCGAGGA GAAGTGTGAG ATCGCCGTTT GTGCGAGCTA TGCTGGCAAT 
AAGATCTGTG ATGGGAAGTG CAACAACCAC GCCTGCGGCT GGGACGGCGG CGACTGCTCG 
CTCAATTTCA ACGACCCCTG GAAGAACTGC TCACAGTCAC TGCAGTGCTG GAAGTACTTC 
AATGATGGCA AGTGTGACTC GCAGTGCAAC AACGCGGGCT GCCTGTATGA TGGGTTTGAC 
TGCCAGAAGT ACGAGGGGCA GTGCAACCCT CTGTACGACC AGTACTGTAA GGACCACTTC 
TCAGATGGTC ACTGTGACCA GGGCTGCAAT AACTTTGAGT GTGAGTGGGA CGGGCTGGAC 
TGTGCAAACA ACATGCCCGA GAAGCTGGCG GATGGCACGC TGGTGGTGGT GGTCCTCATC 
ACCCCTGAGA ACCTGAAGAA CAATTCCTTC AATTTCCTGA GGGAGCTGAG CCGTGTGCTG 
CACACCAATG TAGTCTTCAA GAAGAACGCC AAGGGAGAGT ACATGATCTT CCCATACTAT 
GGCAACGAGG AGGAGCTGAA AAAGCACTAC ATCAAGAGGT CAACGGAGGA CTGGGCAGAT 
ATGTCCAGTG CTGTCATCAA CAAGGTGAAG AGCAGCCTCT ACTCGCGGGC TGGCAGGAGG 
CAGAAGAGAG AGCTGGATCA GATGGACATC AGAGGATCCA TCGTCTACTT GGAAATTGAC 
AACAGGCAGT GCATCCAGTC GTCCTCCCAG TGCTTCCAGA GCGCAACCGA TGTGGCGGCC 
TTCCTGGGGG CCTTGGCCTC CCTGGGCAAC CTCAACATTC CCTACAAAAT AGAGGCCGTC 
AAGAGTGAAA CGGCGGAGCC CGCGAGGAAC TCCCAGCTGT ATCCTATGTA TGTGGTGGTG 
GCTGCACTGG TCTTGCTTGC CTTCATTGGA GTGGGAGTGC TGGTGTCCCG CAAGCGGCGC 
AGGGAGCATG GCCAGCTCTG GTTCCCAGAG GGCTTCAAAG TGACGGAGTC GAGCAAGAAG 
AAGCGCCGGG AACCACTTGG GGAAGATTCT GTTGGACTGA AACCCCTCAA AAATGCTTCT 
GACGGCACGC TGATGGACGA CAACCAAAAT GAGTGGGGTG ACGAGGAGAC CCTGGACACC 
AAGAAGTTCA GGTTCGAGGA GCAGGCGATG CTGCCCGACA CGGATGACCA GACGGACCAC 
AGGCAGTGGA CTCAGCAGCA CCTGGATGCT GCCGACCTCC GCATCTCCTC CATGGCCCCT 
ACCCCACCAC AGGGCGAGAT CGATGCAGAC TGCATGGATG TCAACGTACG AGGTCCAGAC 
GGCTTCACGC CCCTCATGAT CGCCTCGTGC AGCGGAGGGG GGCTGGAGAC TGGCAACAGC 
GAGGAGGAGG ACGATGCCCC TGCAGTCATC TCAGATTTCA TCTACCAAGG CGCCAGTTTG 
CACAACCAGA CTGACCGCAC CGGCGAGACC GCGCTGCACC TGGCTGCCCG CTACTCGCGC 
TCCGACGCTG CCAAACGCCT GCTGGAAGCC AGTGCTGATG CGAACATTCA GGATAACATG 
GGCAGGACCC CTCTGCACGC CGCTGTCTCT GCTGATGCCC AAGGAGTCTT CCAGATCCTG 
ATAAGGAACA GGGCGACTGA TCTCGATGCC CGAATGCACG ATGGGACGAC CCCACTGATC 
CTGGCTGCTC GCTTGGCTGT GGAGGGGATG CTGGAGGACC TCATCAACTG CCACGCAGAC 
GTCAACGCTG TGGATGATCT GGGCAAGTCA GCACTGCACT GGGCAGCTGC TGTGAACAAT 
GTCGAAGCCG CTGTCGTCCT CCTGAAGAAC GGTGCCAATA AGGATATGCA GAACAATAAG 
GAGGAGACCC CGCTGTTCCT CGCCGCCAGA GAAGGGAGCT ACGAAACAGC CAAGGTTCTG 
CTGGACCACT TTGCGAACCG CGACATCACG GACCACATGG ACCGGCTGCC GCGGGACATC 
GCGCAGGAGC GCATGCACCA CGACATCGTG CGGCTGCTGG ATGAGTACAA CCTGGTGCGG 
AGCCCTCCGC TGCACAGCGG CCCTCTCGGG GCCCCCACGC TGTCCCCCCC GCTCTGCTCC 
CCCAGCAGTT ACATCGGCAA CCTGAAGCCG GCCGTGCAGG GCAAGAAGGC CAGGAAGCCG 
AGCACCAAGG GCCTGAGCTG CAACGGCAAG GATTCCAAAG ACCTGAAAGC CCGGAGGAAA 
AAATCACAAG ATGGAAAAGG CTGTCTGCTC GACAACTCCA GCGTGCTGTC CCCAGTGGAC 
TCCCTGGAGT CACCCCACGG GTACCTGTCC GACGTCGCCT CCCCTCCGCT GATGACTTCT 
CCGTTCCAGC AGTCCCCTTC CATGCCTCTG AACCACCTGC CTGGCATGCC CGACGCCCAC 
ATGAGCATCA ACCACCTCAA CATGGCAGGG AAGCAGGAGA TGGCCCTGGG CGGCTCTGGC 
AGGATGGCCT TCGAGGCGGT GCCGCCGCGC CTCTCGCACC TCCCCGTCTC CAGCCCCAGC 
ACGGCGATGA GCAACGCCCC GATGAATTTC TCCGTCGGCG GAGCTGCCGG CCTGAGCGGG 
CAGTGCGACT GGCTGAGCCG GCTGCAGAGC GGCATGGTGC AGAACCAGTA CGGCGCCATG 
CGGGGCGGCA TGCAGCCGGG CACGCACCAG CAAGCACAGA ACCTGCAGCA CGGCATGATG 
AGCTCCCTGC ACAACGGGCT GCCCAGCACC AGCCTGTCGC AGATGATGAG CTACCAGGCC 
ATGCCCAGCA CCCGGCTGGC CTCCCAGCCC CACCTGCTGC AGAACCAGCA GATGCAGCAG 
ATGCAGCAGC CCGGAATGCA GCCGCAGCCC GGAATGCAGC CGCAGCCCGG CATGCAGCAG 
CCTCAACAGC AGCCCCAGCA ACAGCCCCAG CCGCAGCAGC ACCACAACCC CGGCTCCAAC 
GCCAGCGGCC ACATGGGCCA AAATTTCCTC GGTACTGAGC TGAGCCAGCC CGACATGCAG 
CCGGTGAGCA GCAGCGCCAT GGCGGTGCAC ACCATCCTGC CCCAAGATTC GCAGCTGCTA 
CCCACCTCTC TGCCGTCCTC CCTCGCGCAG CCCATGACCA CCACGCAGTT CCTGACCCCC 
CCTTCCCAGC ACAGCTATTC CTCCCCGTTG GACAACACCC CCAGCCACCA GCTCCAGGTG 
CCCGACCACC CCTTCCTCAC TCCCTCTCCG GAGTCTCCAG ACCAGTGGTC GAGCTCCTCG 
CCCCACTCCA ACGTGTCCGA CTGGTCCGAG GGCATCTCCA GCCCCCCCAC CAGCATGCAG 
TCGCAGATGG GACACATCCC CGAGGCCTTC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015134810.1
CDS191..7846
Translation

Target ORF information:

RefSeq Version XM_015279324.2
Organism Gallus gallus(chicken)
Definition Gallus gallus notch 1 (NOTCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015279324.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGTGCA CTGTGAGATC 
AATGTGGATG ACTGCAGCCC TTTCTTTGAT CCCGTCACCC TGGGGCCCAA GTGCTTTAAC 
AATGGCAAGT GCACGGATCG GGTAGGTGGC TACAGCTGCA TCTGCCCCCC TGGCTTTGTA 
GGGGAGCGCT GCGAGGGAGA CGTCAACGAG TGCCTCTCCA ACCCCTGCGA CGCGCGCGGC 
ACCCAGAACT GCGTGCAGCG GGTCAATGAC TACAAATGCG AGTGCCGGCC TGGCTACGCA 
GGGCGTCGCT GTGACACCGT GGTGGATGGC TGCAAGGGCA AACCCTGCAG GAATGGGGGA 
ACGTGCGCTG TGGCCAGCAA CACCGGCCGC GGCTTCATCT GCAAGTGCCC CCCGGGCTTT 
GTGGGTGCCA CCTGCGAGAA CGACTCCCAC ACCTGCGGGA CCCTGCACTG CCTGAACGGT 
GGCACCTGCA TCTCCATGCA CAAGAGCTCC AAGTGTGTGT GCGCAGCGGC CTTCACGGGC 
CCCGAGTGCC AATACCCGGC CAGCAGCCCC TGCATCTCCA ACCCCTGCTA CAACGGGGGC 
ACCTGCGAGT TCCTCAGCGA CGCCTCCCCT TACTACCACT GCAACTGCCC CGCCAACTTC 
AACGGCCTCA ACTGCCACAT CCTCGACTTT GATTTCCAAG GCGGCTTCGG GCAGGACATC 
ATCCCACCCA AAATCGAGGA GAAGTGTGAG ATCGCCGTTT GTGCGAGCTA TGCTGGCAAT 
AAGATCTGTG ATGGGAAGTG CAACAACCAC GCCTGCGGCT GGGACGGCGG CGACTGCTCG 
CTCAATTTCA ACGACCCCTG GAAGAACTGC TCACAGTCAC TGCAGTGCTG GAAGTACTTC 
AATGATGGCA AGTGTGACTC GCAGTGCAAC AACGCGGGCT GCCTGTATGA TGGGTTTGAC 
TGCCAGAAGT ACGAGGGGCA GTGCAACCCT CTGTACGACC AGTACTGTAA GGACCACTTC 
TCAGATGGTC ACTGTGACCA GGGCTGCAAT AACTTTGAGT GTGAGTGGGA CGGGCTGGAC 
TGTGCAAACA ACATGCCCGA GAAGCTGGCG GATGGCACGC TGGTGGTGGT GGTCCTCATC 
ACCCCTGAGA ACCTGAAGAA CAATTCCTTC AATTTCCTGA GGGAGCTGAG CCGTGTGCTG 
CACACCAATG TAGTCTTCAA GAAGAACGCC AAGGGAGAGT ACATGATCTT CCCATACTAT 
GGCAACGAGG AGGAGCTGAA AAAGCACTAC ATCAAGAGGT CAACGGAGGA CTGGGCAGAT 
ATGTCCAGTG CTGTCATCAA CAAGGTGAAG AGCAGCCTCT ACTCGCGGGC TGGCAGGAGG 
CAGAAGAGAG AGCTGGATCA GATGGACATC AGAGGATCCA TCGTCTACTT GGAAATTGAC 
AACAGGCAGT GCATCCAGTC GTCCTCCCAG TGCTTCCAGA GCGCAACCGA TGTGGCGGCC 
TTCCTGGGGG CCTTGGCCTC CCTGGGCAAC CTCAACATTC CCTACAAAAT AGAGGCCGTC 
AAGAGTGAAA CGGCGGAGCC CGCGAGGAAC TCCCAGCTGT ATCCTATGTA TGTGGTGGTG 
GCTGCACTGG TCTTGCTTGC CTTCATTGGA GTGGGAGTGC TGGTGTCCCG CAAGCGGCGC 
AGGGAGCATG GCCAGCTCTG GTTCCCAGAG GGCTTCAAAG TGACGGAGTC GAGCAAGAAG 
AAGCGCCGGG AACCACTTGG GGAAGATTCT GTTGGACTGA AACCCCTCAA AAATGCTTCT 
GACGGCACGC TGATGGACGA CAACCAAAAT GAGTGGGGTG ACGAGGAGAC CCTGGACACC 
AAGAAGTTCA GGTTCGAGGA GCAGGCGATG CTGCCCGACA CGGATGACCA GACGGACCAC 
AGGCAGTGGA CTCAGCAGCA CCTGGATGCT GCCGACCTCC GCATCTCCTC CATGGCCCCT 
ACCCCACCAC AGGGCGAGAT CGATGCAGAC TGCATGGATG TCAACGTACG AGGTCCAGAC 
GGCTTCACGC CCCTCATGAT CGCCTCGTGC AGCGGAGGGG GGCTGGAGAC TGGCAACAGC 
GAGGAGGAGG ACGATGCCCC TGCAGTCATC TCAGATTTCA TCTACCAAGG CGCCAGTTTG 
CACAACCAGA CTGACCGCAC CGGCGAGACC GCGCTGCACC TGGCTGCCCG CTACTCGCGC 
TCCGACGCTG CCAAACGCCT GCTGGAAGCC AGTGCTGATG CGAACATTCA GGATAACATG 
GGCAGGACCC CTCTGCACGC CGCTGTCTCT GCTGATGCCC AAGGAGTCTT CCAGATCCTG 
ATAAGGAACA GGGCGACTGA TCTCGATGCC CGAATGCACG ATGGGACGAC CCCACTGATC 
CTGGCTGCTC GCTTGGCTGT GGAGGGGATG CTGGAGGACC TCATCAACTG CCACGCAGAC 
GTCAACGCTG TGGATGATCT GGGCAAGTCA GCACTGCACT GGGCAGCTGC TGTGAACAAT 
GTCGAAGCCG CTGTCGTCCT CCTGAAGAAC GGTGCCAATA AGGATATGCA GAACAATAAG 
GAGGAGACCC CGCTGTTCCT CGCCGCCAGA GAAGGGAGCT ACGAAACAGC CAAGGTTCTG 
CTGGACCACT TTGCGAACCG CGACATCACG GACCACATGG ACCGGCTGCC GCGGGACATC 
GCGCAGGAGC GCATGCACCA CGACATCGTG CGGCTGCTGG ATGAGTACAA CCTGGTGCGG 
AGCCCTCCGC TGCACAGCGG CCCTCTCGGG GCCCCCACGC TGTCCCCCCC GCTCTGCTCC 
CCCAGCAGTT ACATCGGCAA CCTGAAGCCG GCCGTGCAGG GCAAGAAGGC CAGGAAGCCG 
AGCACCAAGG GCCTGAGCTG CAACGGCAAG GATTCCAAAG ACCTGAAAGC CCGGAGGAAA 
AAATCACAAG ATGGAAAAGG CTGTCTGCTC GACAACTCCA GCGTGCTGTC CCCAGTGGAC 
TCCCTGGAGT CACCCCACGG GTACCTGTCC GACGTCGCCT CCCCTCCGCT GATGACTTCT 
CCGTTCCAGC AGTCCCCTTC CATGCCTCTG AACCACCTGC CTGGCATGCC CGACGCCCAC 
ATGAGCATCA ACCACCTCAA CATGGCAGGG AAGCAGGAGA TGGCCCTGGG CGGCTCTGGC 
AGGATGGCCT TCGAGGCGGT GCCGCCGCGC CTCTCGCACC TCCCCGTCTC CAGCCCCAGC 
ACGGCGATGA GCAACGCCCC GATGAATTTC TCCGTCGGCG GAGCTGCCGG CCTGAGCGGG 
CAGTGCGACT GGCTGAGCCG GCTGCAGAGC GGCATGGTGC AGAACCAGTA CGGCGCCATG 
CGGGGCGGCA TGCAGCCGGG CACGCACCAG CAAGCACAGA ACCTGCAGCA CGGCATGATG 
AGCTCCCTGC ACAACGGGCT GCCCAGCACC AGCCTGTCGC AGATGATGAG CTACCAGGCC 
ATGCCCAGCA CCCGGCTGGC CTCCCAGCCC CACCTGCTGC AGAACCAGCA GATGCAGCAG 
ATGCAGCAGC CCGGAATGCA GCCGCAGCCC GGAATGCAGC CGCAGCCCGG CATGCAGCAG 
CCTCAACAGC AGCCCCAGCA ACAGCCCCAG CCGCAGCAGC ACCACAACCC CGGCTCCAAC 
GCCAGCGGCC ACATGGGCCA AAATTTCCTC GGTACTGAGC TGAGCCAGCC CGACATGCAG 
CCGGTGAGCA GCAGCGCCAT GGCGGTGCAC ACCATCCTGC CCCAAGATTC GCAGCTGCTA 
CCCACCTCTC TGCCGTCCTC CCTCGCGCAG CCCATGACCA CCACGCAGTT CCTGACCCCC 
CCTTCCCAGC ACAGCTATTC CTCCCCGTTG GACAACACCC CCAGCCACCA GCTCCAGGTG 
CCCGACCACC CCTTCCTCAC TCCCTCTCCG GAGTCTCCAG ACCAGTGGTC GAGCTCCTCG 
CCCCACTCCA ACGTGTCCGA CTGGTCCGAG GGCATCTCCA GCCCCCCCAC CAGCATGCAG 
TCGCAGATGG GACACATCCC CGAGGCCTTC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa43347
Clone ID Related Accession (Same CDS sequence) XM_015279325.2 , XM_015279325.1
Accession Version XM_015279325.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 7518bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product neurogenic locus notch homolog protein 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGAGCG CTGCGAGGGA 
GACGTCAACG AGTGCCTCTC CAACCCCTGC GACGCGCGCG GCACCCAGAA CTGCGTGCAG 
CGGGTCAATG ACTACAAATG CGAGTGCCGG CCTGGCTACG CAGGGCGTCG CTGTGACACC 
GTGGTGGATG GCTGCAAGGG CAAACCCTGC AGGAATGGGG GAACGTGCGC TGTGGCCAGC 
AACACCGGCC GCGGCTTCAT CTGCAAGTGC CCCCCGGGCT TTGTGGGTGC CACCTGCGAG 
AACGACTCCC ACACCTGCGG GACCCTGCAC TGCCTGAACG GTGGCACCTG CATCTCCATG 
CACAAGAGCT CCAAGTGTGT GTGCGCAGCG GCCTTCACGG GCCCCGAGTG CCAATACCCG 
GCCAGCAGCC CCTGCATCTC CAACCCCTGC TACAACGGGG GCACCTGCGA GTTCCTCAGC 
GACGCCTCCC CTTACTACCA CTGCAACTGC CCCGCCAACT TCAACGGCCT CAACTGCCAC 
ATCCTCGACT TTGATTTCCA AGGCGGCTTC GGGCAGGACA TCATCCCACC CAAAATCGAG 
GAGAAGTGTG AGATCGCCGT TTGTGCGAGC TATGCTGGCA ATAAGATCTG TGATGGGAAG 
TGCAACAACC ACGCCTGCGG CTGGGACGGC GGCGACTGCT CGCTCAATTT CAACGACCCC 
TGGAAGAACT GCTCACAGTC ACTGCAGTGC TGGAAGTACT TCAATGATGG CAAGTGTGAC 
TCGCAGTGCA ACAACGCGGG CTGCCTGTAT GATGGGTTTG ACTGCCAGAA GTACGAGGGG 
CAGTGCAACC CTCTGTACGA CCAGTACTGT AAGGACCACT TCTCAGATGG TCACTGTGAC 
CAGGGCTGCA ATAACTTTGA GTGTGAGTGG GACGGGCTGG ACTGTGCAAA CAACATGCCC 
GAGAAGCTGG CGGATGGCAC GCTGGTGGTG GTGGTCCTCA TCACCCCTGA GAACCTGAAG 
AACAATTCCT TCAATTTCCT GAGGGAGCTG AGCCGTGTGC TGCACACCAA TGTAGTCTTC 
AAGAAGAACG CCAAGGGAGA GTACATGATC TTCCCATACT ATGGCAACGA GGAGGAGCTG 
AAAAAGCACT ACATCAAGAG GTCAACGGAG GACTGGGCAG ATATGTCCAG TGCTGTCATC 
AACAAGGTGA AGAGCAGCCT CTACTCGCGG GCTGGCAGGA GGCAGAAGAG AGAGCTGGAT 
CAGATGGACA TCAGAGGATC CATCGTCTAC TTGGAAATTG ACAACAGGCA GTGCATCCAG 
TCGTCCTCCC AGTGCTTCCA GAGCGCAACC GATGTGGCGG CCTTCCTGGG GGCCTTGGCC 
TCCCTGGGCA ACCTCAACAT TCCCTACAAA ATAGAGGCCG TCAAGAGTGA AACGGCGGAG 
CCCGCGAGGA ACTCCCAGCT GTATCCTATG TATGTGGTGG TGGCTGCACT GGTCTTGCTT 
GCCTTCATTG GAGTGGGAGT GCTGGTGTCC CGCAAGCGGC GCAGGGAGCA TGGCCAGCTC 
TGGTTCCCAG AGGGCTTCAA AGTGACGGAG TCGAGCAAGA AGAAGCGCCG GGAACCACTT 
GGGGAAGATT CTGTTGGACT GAAACCCCTC AAAAATGCTT CTGACGGCAC GCTGATGGAC 
GACAACCAAA ATGAGTGGGG TGACGAGGAG ACCCTGGACA CCAAGAAGTT CAGGTTCGAG 
GAGCAGGCGA TGCTGCCCGA CACGGATGAC CAGACGGACC ACAGGCAGTG GACTCAGCAG 
CACCTGGATG CTGCCGACCT CCGCATCTCC TCCATGGCCC CTACCCCACC ACAGGGCGAG 
ATCGATGCAG ACTGCATGGA TGTCAACGTA CGAGGTCCAG ACGGCTTCAC GCCCCTCATG 
ATCGCCTCGT GCAGCGGAGG GGGGCTGGAG ACTGGCAACA GCGAGGAGGA GGACGATGCC 
CCTGCAGTCA TCTCAGATTT CATCTACCAA GGCGCCAGTT TGCACAACCA GACTGACCGC 
ACCGGCGAGA CCGCGCTGCA CCTGGCTGCC CGCTACTCGC GCTCCGACGC TGCCAAACGC 
CTGCTGGAAG CCAGTGCTGA TGCGAACATT CAGGATAACA TGGGCAGGAC CCCTCTGCAC 
GCCGCTGTCT CTGCTGATGC CCAAGGAGTC TTCCAGATCC TGATAAGGAA CAGGGCGACT 
GATCTCGATG CCCGAATGCA CGATGGGACG ACCCCACTGA TCCTGGCTGC TCGCTTGGCT 
GTGGAGGGGA TGCTGGAGGA CCTCATCAAC TGCCACGCAG ACGTCAACGC TGTGGATGAT 
CTGGGCAAGT CAGCACTGCA CTGGGCAGCT GCTGTGAACA ATGTCGAAGC CGCTGTCGTC 
CTCCTGAAGA ACGGTGCCAA TAAGGATATG CAGAACAATA AGGAGGAGAC CCCGCTGTTC 
CTCGCCGCCA GAGAAGGGAG CTACGAAACA GCCAAGGTTC TGCTGGACCA CTTTGCGAAC 
CGCGACATCA CGGACCACAT GGACCGGCTG CCGCGGGACA TCGCGCAGGA GCGCATGCAC 
CACGACATCG TGCGGCTGCT GGATGAGTAC AACCTGGTGC GGAGCCCTCC GCTGCACAGC 
GGCCCTCTCG GGGCCCCCAC GCTGTCCCCC CCGCTCTGCT CCCCCAGCAG TTACATCGGC 
AACCTGAAGC CGGCCGTGCA GGGCAAGAAG GCCAGGAAGC CGAGCACCAA GGGCCTGAGC 
TGCAACGGCA AGGATTCCAA AGACCTGAAA GCCCGGAGGA AAAAATCACA AGATGGAAAA 
GGCTGTCTGC TCGACAACTC CAGCGTGCTG TCCCCAGTGG ACTCCCTGGA GTCACCCCAC 
GGGTACCTGT CCGACGTCGC CTCCCCTCCG CTGATGACTT CTCCGTTCCA GCAGTCCCCT 
TCCATGCCTC TGAACCACCT GCCTGGCATG CCCGACGCCC ACATGAGCAT CAACCACCTC 
AACATGGCAG GGAAGCAGGA GATGGCCCTG GGCGGCTCTG GCAGGATGGC CTTCGAGGCG 
GTGCCGCCGC GCCTCTCGCA CCTCCCCGTC TCCAGCCCCA GCACGGCGAT GAGCAACGCC 
CCGATGAATT TCTCCGTCGG CGGAGCTGCC GGCCTGAGCG GGCAGTGCGA CTGGCTGAGC 
CGGCTGCAGA GCGGCATGGT GCAGAACCAG TACGGCGCCA TGCGGGGCGG CATGCAGCCG 
GGCACGCACC AGCAAGCACA GAACCTGCAG CACGGCATGA TGAGCTCCCT GCACAACGGG 
CTGCCCAGCA CCAGCCTGTC GCAGATGATG AGCTACCAGG CCATGCCCAG CACCCGGCTG 
GCCTCCCAGC CCCACCTGCT GCAGAACCAG CAGATGCAGC AGATGCAGCA GCCCGGAATG 
CAGCCGCAGC CCGGAATGCA GCCGCAGCCC GGCATGCAGC AGCCTCAACA GCAGCCCCAG 
CAACAGCCCC AGCCGCAGCA GCACCACAAC CCCGGCTCCA ACGCCAGCGG CCACATGGGC 
CAAAATTTCC TCGGTACTGA GCTGAGCCAG CCCGACATGC AGCCGGTGAG CAGCAGCGCC 
ATGGCGGTGC ACACCATCCT GCCCCAAGAT TCGCAGCTGC TACCCACCTC TCTGCCGTCC 
TCCCTCGCGC AGCCCATGAC CACCACGCAG TTCCTGACCC CCCCTTCCCA GCACAGCTAT 
TCCTCCCCGT TGGACAACAC CCCCAGCCAC CAGCTCCAGG TGCCCGACCA CCCCTTCCTC 
ACTCCCTCTC CGGAGTCTCC AGACCAGTGG TCGAGCTCCT CGCCCCACTC CAACGTGTCC 
GACTGGTCCG AGGGCATCTC CAGCCCCCCC ACCAGCATGC AGTCGCAGAT GGGACACATC 
CCCGAGGCCT TCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015134811.1
CDS191..7708
Translation

Target ORF information:

RefSeq Version XM_015279325.2
Organism Gallus gallus(chicken)
Definition Gallus gallus notch 1 (NOTCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015279325.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGAGCG CTGCGAGGGA 
GACGTCAACG AGTGCCTCTC CAACCCCTGC GACGCGCGCG GCACCCAGAA CTGCGTGCAG 
CGGGTCAATG ACTACAAATG CGAGTGCCGG CCTGGCTACG CAGGGCGTCG CTGTGACACC 
GTGGTGGATG GCTGCAAGGG CAAACCCTGC AGGAATGGGG GAACGTGCGC TGTGGCCAGC 
AACACCGGCC GCGGCTTCAT CTGCAAGTGC CCCCCGGGCT TTGTGGGTGC CACCTGCGAG 
AACGACTCCC ACACCTGCGG GACCCTGCAC TGCCTGAACG GTGGCACCTG CATCTCCATG 
CACAAGAGCT CCAAGTGTGT GTGCGCAGCG GCCTTCACGG GCCCCGAGTG CCAATACCCG 
GCCAGCAGCC CCTGCATCTC CAACCCCTGC TACAACGGGG GCACCTGCGA GTTCCTCAGC 
GACGCCTCCC CTTACTACCA CTGCAACTGC CCCGCCAACT TCAACGGCCT CAACTGCCAC 
ATCCTCGACT TTGATTTCCA AGGCGGCTTC GGGCAGGACA TCATCCCACC CAAAATCGAG 
GAGAAGTGTG AGATCGCCGT TTGTGCGAGC TATGCTGGCA ATAAGATCTG TGATGGGAAG 
TGCAACAACC ACGCCTGCGG CTGGGACGGC GGCGACTGCT CGCTCAATTT CAACGACCCC 
TGGAAGAACT GCTCACAGTC ACTGCAGTGC TGGAAGTACT TCAATGATGG CAAGTGTGAC 
TCGCAGTGCA ACAACGCGGG CTGCCTGTAT GATGGGTTTG ACTGCCAGAA GTACGAGGGG 
CAGTGCAACC CTCTGTACGA CCAGTACTGT AAGGACCACT TCTCAGATGG TCACTGTGAC 
CAGGGCTGCA ATAACTTTGA GTGTGAGTGG GACGGGCTGG ACTGTGCAAA CAACATGCCC 
GAGAAGCTGG CGGATGGCAC GCTGGTGGTG GTGGTCCTCA TCACCCCTGA GAACCTGAAG 
AACAATTCCT TCAATTTCCT GAGGGAGCTG AGCCGTGTGC TGCACACCAA TGTAGTCTTC 
AAGAAGAACG CCAAGGGAGA GTACATGATC TTCCCATACT ATGGCAACGA GGAGGAGCTG 
AAAAAGCACT ACATCAAGAG GTCAACGGAG GACTGGGCAG ATATGTCCAG TGCTGTCATC 
AACAAGGTGA AGAGCAGCCT CTACTCGCGG GCTGGCAGGA GGCAGAAGAG AGAGCTGGAT 
CAGATGGACA TCAGAGGATC CATCGTCTAC TTGGAAATTG ACAACAGGCA GTGCATCCAG 
TCGTCCTCCC AGTGCTTCCA GAGCGCAACC GATGTGGCGG CCTTCCTGGG GGCCTTGGCC 
TCCCTGGGCA ACCTCAACAT TCCCTACAAA ATAGAGGCCG TCAAGAGTGA AACGGCGGAG 
CCCGCGAGGA ACTCCCAGCT GTATCCTATG TATGTGGTGG TGGCTGCACT GGTCTTGCTT 
GCCTTCATTG GAGTGGGAGT GCTGGTGTCC CGCAAGCGGC GCAGGGAGCA TGGCCAGCTC 
TGGTTCCCAG AGGGCTTCAA AGTGACGGAG TCGAGCAAGA AGAAGCGCCG GGAACCACTT 
GGGGAAGATT CTGTTGGACT GAAACCCCTC AAAAATGCTT CTGACGGCAC GCTGATGGAC 
GACAACCAAA ATGAGTGGGG TGACGAGGAG ACCCTGGACA CCAAGAAGTT CAGGTTCGAG 
GAGCAGGCGA TGCTGCCCGA CACGGATGAC CAGACGGACC ACAGGCAGTG GACTCAGCAG 
CACCTGGATG CTGCCGACCT CCGCATCTCC TCCATGGCCC CTACCCCACC ACAGGGCGAG 
ATCGATGCAG ACTGCATGGA TGTCAACGTA CGAGGTCCAG ACGGCTTCAC GCCCCTCATG 
ATCGCCTCGT GCAGCGGAGG GGGGCTGGAG ACTGGCAACA GCGAGGAGGA GGACGATGCC 
CCTGCAGTCA TCTCAGATTT CATCTACCAA GGCGCCAGTT TGCACAACCA GACTGACCGC 
ACCGGCGAGA CCGCGCTGCA CCTGGCTGCC CGCTACTCGC GCTCCGACGC TGCCAAACGC 
CTGCTGGAAG CCAGTGCTGA TGCGAACATT CAGGATAACA TGGGCAGGAC CCCTCTGCAC 
GCCGCTGTCT CTGCTGATGC CCAAGGAGTC TTCCAGATCC TGATAAGGAA CAGGGCGACT 
GATCTCGATG CCCGAATGCA CGATGGGACG ACCCCACTGA TCCTGGCTGC TCGCTTGGCT 
GTGGAGGGGA TGCTGGAGGA CCTCATCAAC TGCCACGCAG ACGTCAACGC TGTGGATGAT 
CTGGGCAAGT CAGCACTGCA CTGGGCAGCT GCTGTGAACA ATGTCGAAGC CGCTGTCGTC 
CTCCTGAAGA ACGGTGCCAA TAAGGATATG CAGAACAATA AGGAGGAGAC CCCGCTGTTC 
CTCGCCGCCA GAGAAGGGAG CTACGAAACA GCCAAGGTTC TGCTGGACCA CTTTGCGAAC 
CGCGACATCA CGGACCACAT GGACCGGCTG CCGCGGGACA TCGCGCAGGA GCGCATGCAC 
CACGACATCG TGCGGCTGCT GGATGAGTAC AACCTGGTGC GGAGCCCTCC GCTGCACAGC 
GGCCCTCTCG GGGCCCCCAC GCTGTCCCCC CCGCTCTGCT CCCCCAGCAG TTACATCGGC 
AACCTGAAGC CGGCCGTGCA GGGCAAGAAG GCCAGGAAGC CGAGCACCAA GGGCCTGAGC 
TGCAACGGCA AGGATTCCAA AGACCTGAAA GCCCGGAGGA AAAAATCACA AGATGGAAAA 
GGCTGTCTGC TCGACAACTC CAGCGTGCTG TCCCCAGTGG ACTCCCTGGA GTCACCCCAC 
GGGTACCTGT CCGACGTCGC CTCCCCTCCG CTGATGACTT CTCCGTTCCA GCAGTCCCCT 
TCCATGCCTC TGAACCACCT GCCTGGCATG CCCGACGCCC ACATGAGCAT CAACCACCTC 
AACATGGCAG GGAAGCAGGA GATGGCCCTG GGCGGCTCTG GCAGGATGGC CTTCGAGGCG 
GTGCCGCCGC GCCTCTCGCA CCTCCCCGTC TCCAGCCCCA GCACGGCGAT GAGCAACGCC 
CCGATGAATT TCTCCGTCGG CGGAGCTGCC GGCCTGAGCG GGCAGTGCGA CTGGCTGAGC 
CGGCTGCAGA GCGGCATGGT GCAGAACCAG TACGGCGCCA TGCGGGGCGG CATGCAGCCG 
GGCACGCACC AGCAAGCACA GAACCTGCAG CACGGCATGA TGAGCTCCCT GCACAACGGG 
CTGCCCAGCA CCAGCCTGTC GCAGATGATG AGCTACCAGG CCATGCCCAG CACCCGGCTG 
GCCTCCCAGC CCCACCTGCT GCAGAACCAG CAGATGCAGC AGATGCAGCA GCCCGGAATG 
CAGCCGCAGC CCGGAATGCA GCCGCAGCCC GGCATGCAGC AGCCTCAACA GCAGCCCCAG 
CAACAGCCCC AGCCGCAGCA GCACCACAAC CCCGGCTCCA ACGCCAGCGG CCACATGGGC 
CAAAATTTCC TCGGTACTGA GCTGAGCCAG CCCGACATGC AGCCGGTGAG CAGCAGCGCC 
ATGGCGGTGC ACACCATCCT GCCCCAAGAT TCGCAGCTGC TACCCACCTC TCTGCCGTCC 
TCCCTCGCGC AGCCCATGAC CACCACGCAG TTCCTGACCC CCCCTTCCCA GCACAGCTAT 
TCCTCCCCGT TGGACAACAC CCCCAGCCAC CAGCTCCAGG TGCCCGACCA CCCCTTCCTC 
ACTCCCTCTC CGGAGTCTCC AGACCAGTGG TCGAGCTCCT CGCCCCACTC CAACGTGTCC 
GACTGGTCCG AGGGCATCTC CAGCCCCCCC ACCAGCATGC AGTCGCAGAT GGGACACATC 
CCCGAGGCCT TCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa43347
Clone ID Related Accession (Same CDS sequence) XM_015279325.2 , XM_015279325.1
Accession Version XM_015279325.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 7518bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product neurogenic locus notch homolog protein 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGAGCG CTGCGAGGGA 
GACGTCAACG AGTGCCTCTC CAACCCCTGC GACGCGCGCG GCACCCAGAA CTGCGTGCAG 
CGGGTCAATG ACTACAAATG CGAGTGCCGG CCTGGCTACG CAGGGCGTCG CTGTGACACC 
GTGGTGGATG GCTGCAAGGG CAAACCCTGC AGGAATGGGG GAACGTGCGC TGTGGCCAGC 
AACACCGGCC GCGGCTTCAT CTGCAAGTGC CCCCCGGGCT TTGTGGGTGC CACCTGCGAG 
AACGACTCCC ACACCTGCGG GACCCTGCAC TGCCTGAACG GTGGCACCTG CATCTCCATG 
CACAAGAGCT CCAAGTGTGT GTGCGCAGCG GCCTTCACGG GCCCCGAGTG CCAATACCCG 
GCCAGCAGCC CCTGCATCTC CAACCCCTGC TACAACGGGG GCACCTGCGA GTTCCTCAGC 
GACGCCTCCC CTTACTACCA CTGCAACTGC CCCGCCAACT TCAACGGCCT CAACTGCCAC 
ATCCTCGACT TTGATTTCCA AGGCGGCTTC GGGCAGGACA TCATCCCACC CAAAATCGAG 
GAGAAGTGTG AGATCGCCGT TTGTGCGAGC TATGCTGGCA ATAAGATCTG TGATGGGAAG 
TGCAACAACC ACGCCTGCGG CTGGGACGGC GGCGACTGCT CGCTCAATTT CAACGACCCC 
TGGAAGAACT GCTCACAGTC ACTGCAGTGC TGGAAGTACT TCAATGATGG CAAGTGTGAC 
TCGCAGTGCA ACAACGCGGG CTGCCTGTAT GATGGGTTTG ACTGCCAGAA GTACGAGGGG 
CAGTGCAACC CTCTGTACGA CCAGTACTGT AAGGACCACT TCTCAGATGG TCACTGTGAC 
CAGGGCTGCA ATAACTTTGA GTGTGAGTGG GACGGGCTGG ACTGTGCAAA CAACATGCCC 
GAGAAGCTGG CGGATGGCAC GCTGGTGGTG GTGGTCCTCA TCACCCCTGA GAACCTGAAG 
AACAATTCCT TCAATTTCCT GAGGGAGCTG AGCCGTGTGC TGCACACCAA TGTAGTCTTC 
AAGAAGAACG CCAAGGGAGA GTACATGATC TTCCCATACT ATGGCAACGA GGAGGAGCTG 
AAAAAGCACT ACATCAAGAG GTCAACGGAG GACTGGGCAG ATATGTCCAG TGCTGTCATC 
AACAAGGTGA AGAGCAGCCT CTACTCGCGG GCTGGCAGGA GGCAGAAGAG AGAGCTGGAT 
CAGATGGACA TCAGAGGATC CATCGTCTAC TTGGAAATTG ACAACAGGCA GTGCATCCAG 
TCGTCCTCCC AGTGCTTCCA GAGCGCAACC GATGTGGCGG CCTTCCTGGG GGCCTTGGCC 
TCCCTGGGCA ACCTCAACAT TCCCTACAAA ATAGAGGCCG TCAAGAGTGA AACGGCGGAG 
CCCGCGAGGA ACTCCCAGCT GTATCCTATG TATGTGGTGG TGGCTGCACT GGTCTTGCTT 
GCCTTCATTG GAGTGGGAGT GCTGGTGTCC CGCAAGCGGC GCAGGGAGCA TGGCCAGCTC 
TGGTTCCCAG AGGGCTTCAA AGTGACGGAG TCGAGCAAGA AGAAGCGCCG GGAACCACTT 
GGGGAAGATT CTGTTGGACT GAAACCCCTC AAAAATGCTT CTGACGGCAC GCTGATGGAC 
GACAACCAAA ATGAGTGGGG TGACGAGGAG ACCCTGGACA CCAAGAAGTT CAGGTTCGAG 
GAGCAGGCGA TGCTGCCCGA CACGGATGAC CAGACGGACC ACAGGCAGTG GACTCAGCAG 
CACCTGGATG CTGCCGACCT CCGCATCTCC TCCATGGCCC CTACCCCACC ACAGGGCGAG 
ATCGATGCAG ACTGCATGGA TGTCAACGTA CGAGGTCCAG ACGGCTTCAC GCCCCTCATG 
ATCGCCTCGT GCAGCGGAGG GGGGCTGGAG ACTGGCAACA GCGAGGAGGA GGACGATGCC 
CCTGCAGTCA TCTCAGATTT CATCTACCAA GGCGCCAGTT TGCACAACCA GACTGACCGC 
ACCGGCGAGA CCGCGCTGCA CCTGGCTGCC CGCTACTCGC GCTCCGACGC TGCCAAACGC 
CTGCTGGAAG CCAGTGCTGA TGCGAACATT CAGGATAACA TGGGCAGGAC CCCTCTGCAC 
GCCGCTGTCT CTGCTGATGC CCAAGGAGTC TTCCAGATCC TGATAAGGAA CAGGGCGACT 
GATCTCGATG CCCGAATGCA CGATGGGACG ACCCCACTGA TCCTGGCTGC TCGCTTGGCT 
GTGGAGGGGA TGCTGGAGGA CCTCATCAAC TGCCACGCAG ACGTCAACGC TGTGGATGAT 
CTGGGCAAGT CAGCACTGCA CTGGGCAGCT GCTGTGAACA ATGTCGAAGC CGCTGTCGTC 
CTCCTGAAGA ACGGTGCCAA TAAGGATATG CAGAACAATA AGGAGGAGAC CCCGCTGTTC 
CTCGCCGCCA GAGAAGGGAG CTACGAAACA GCCAAGGTTC TGCTGGACCA CTTTGCGAAC 
CGCGACATCA CGGACCACAT GGACCGGCTG CCGCGGGACA TCGCGCAGGA GCGCATGCAC 
CACGACATCG TGCGGCTGCT GGATGAGTAC AACCTGGTGC GGAGCCCTCC GCTGCACAGC 
GGCCCTCTCG GGGCCCCCAC GCTGTCCCCC CCGCTCTGCT CCCCCAGCAG TTACATCGGC 
AACCTGAAGC CGGCCGTGCA GGGCAAGAAG GCCAGGAAGC CGAGCACCAA GGGCCTGAGC 
TGCAACGGCA AGGATTCCAA AGACCTGAAA GCCCGGAGGA AAAAATCACA AGATGGAAAA 
GGCTGTCTGC TCGACAACTC CAGCGTGCTG TCCCCAGTGG ACTCCCTGGA GTCACCCCAC 
GGGTACCTGT CCGACGTCGC CTCCCCTCCG CTGATGACTT CTCCGTTCCA GCAGTCCCCT 
TCCATGCCTC TGAACCACCT GCCTGGCATG CCCGACGCCC ACATGAGCAT CAACCACCTC 
AACATGGCAG GGAAGCAGGA GATGGCCCTG GGCGGCTCTG GCAGGATGGC CTTCGAGGCG 
GTGCCGCCGC GCCTCTCGCA CCTCCCCGTC TCCAGCCCCA GCACGGCGAT GAGCAACGCC 
CCGATGAATT TCTCCGTCGG CGGAGCTGCC GGCCTGAGCG GGCAGTGCGA CTGGCTGAGC 
CGGCTGCAGA GCGGCATGGT GCAGAACCAG TACGGCGCCA TGCGGGGCGG CATGCAGCCG 
GGCACGCACC AGCAAGCACA GAACCTGCAG CACGGCATGA TGAGCTCCCT GCACAACGGG 
CTGCCCAGCA CCAGCCTGTC GCAGATGATG AGCTACCAGG CCATGCCCAG CACCCGGCTG 
GCCTCCCAGC CCCACCTGCT GCAGAACCAG CAGATGCAGC AGATGCAGCA GCCCGGAATG 
CAGCCGCAGC CCGGAATGCA GCCGCAGCCC GGCATGCAGC AGCCTCAACA GCAGCCCCAG 
CAACAGCCCC AGCCGCAGCA GCACCACAAC CCCGGCTCCA ACGCCAGCGG CCACATGGGC 
CAAAATTTCC TCGGTACTGA GCTGAGCCAG CCCGACATGC AGCCGGTGAG CAGCAGCGCC 
ATGGCGGTGC ACACCATCCT GCCCCAAGAT TCGCAGCTGC TACCCACCTC TCTGCCGTCC 
TCCCTCGCGC AGCCCATGAC CACCACGCAG TTCCTGACCC CCCCTTCCCA GCACAGCTAT 
TCCTCCCCGT TGGACAACAC CCCCAGCCAC CAGCTCCAGG TGCCCGACCA CCCCTTCCTC 
ACTCCCTCTC CGGAGTCTCC AGACCAGTGG TCGAGCTCCT CGCCCCACTC CAACGTGTCC 
GACTGGTCCG AGGGCATCTC CAGCCCCCCC ACCAGCATGC AGTCGCAGAT GGGACACATC 
CCCGAGGCCT TCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015134811.1
CDS189..7706
Translation

Target ORF information:

RefSeq Version XM_015279325.1
Organism Gallus gallus(chicken)
Definition Gallus gallus notch 1 (NOTCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015279325.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
ATGAAGCGGC TCTTGGCCAC CGGGCTCCTG CTGGCCCTGC TGCCCGCCCC GACGCGAGGT 
GTGCGGTGCA CGCAGCTGGC CGAATCCTGC CTCAACGGGG GCAAGTGTGA AACTTTCCTC 
AATGGGACGG AGGTGTGCCA GTGCAGCAGT GCCCATATGG GCGAGCGGTG CCAGCTGCCC 
AACCCCTGCC TCAGCTCCCC CTGCAAGAAC GCCGGCACCT GCATCCCCCT GCTGCGTGGC 
AGCACCGCCG ACTACACCTG TGTCTGCCGC CTGGGCTTCA CCGACGAGCT GTGCCTCACA 
CCCCTGGACA ACGCCTGCCT CAACAACCCC TGCCGCAACG GGGGCACCTG TGACCTGGTG 
ACACTCAGCG AGTACAAGTG CCGCTGCCCG CCGGGCTGGT CAGGTAAAAC CTGCCAGCAG 
GCCGACCCCT GTGCCTCCAA CCCCTGTGCC AACGGGGGTC AGTGCGTCCC CTTCGAAGCC 
CACTACATCT GCCGCTGCAC GGCCGGCTTC CACGGGGCCA ACTGCAAGCA GGACGTGAAC 
GAATGCAACA TCTCACCACC AGTCTGCAAG AACGGTGGCA GCTGCACCAA CGAGGTGGGC 
ACCTACCAGT GCTCCTGCAA GCCAGCCTAC ACCGGGCAGA ACTGCGAGCA TCTCTATGTG 
CCCTGCAACC CCTCGCCCTG CCAGAATGGG GGCACGTGCC GCCAAACTGG GGACACCACC 
TACGACTGCA CCTGTCTGCC AGGGTTCACC GGGCAGAACT GCGAGGAGAA CATCGATGAC 
TGTCCAGGGA ACAACTGCAG GAATGGGGGC ACCTGCGTGG ATGGCGTCAA CACCTACAAC 
TGCCAGTGCC CGCCCGAGTG GACAGGTCAG TACTGCACCG AGGATGTGGA TGAGTGCCAG 
CTGATGCCCA ACGCCTGCCA GAACGGGGGC ACCTGCCACA ACAACCACGG CGGCTACAAC 
TGCGTCTGCG TCAATGGCTG GACGGGTGAG GACTGCAGTG AGAACATCGA TGACTGCGCC 
ATGGCTGCCT GCTTCCAGGG GGCCACCTGC CATGACCGGG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GGCGCACAGG TTTGCTGTGC CACCTGGATG ACGCCTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG CGATACCAAC CCTGTCAATG GCAAAGCCAT CTGCACGTGT 
CCTTCGGGGT ACATGGGGCC GGCGTGCAAC CAGGACGTGG ATGAGTGCTC ACTGGGAGCC 
AACCCCTGTG AGCACGCAGG GAAATGCATC AACACCCAGG GGTCCTTCCA ATGCCAGTGT 
CTGCAGGGCT ACTCGGGCCC CCGCTGCGAG ATCGATGTCA ATGAGTGCCT CTCCAACCCC 
TGCCAGAACG ATGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCT 
GGATACGAGG GCGTCTACTG TGAAATCAAC ACAGATGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCA ACTGCCTGGA TAAGATCAAC GAGTTCCACT GCGAGTGCCC CACTGGGTTC 
AACGGGCACC TGTGCCAGTT TGACATCGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGG 
GCCAAGTGCG TGGATGGCCC CAACACCTAC AGCTGCGAGT GCACCGAAGG TTTCTCAGGT 
GTTCACTGTG AGATCGACAT CGATGAGTGC AACCCCGACC CGTGCCACTA TGGGACCTGC 
AAGGACAGCA TCGCCGCCTT CACCTGCCTC TGCCAGCCCG GCTACACGGG CCACCGCTGC 
GACATCAACA TCAATGAGTG CCAGAGCCAG CCCTGCAGAA ACGGGGGGAC CTGTCAGGAC 
AGGGACAACG CCTACAACTG TCTGTGCCTC AAGGGGACCA CGGGGCCCAA CTGCGAGATC 
AACCTGGACG ACTGCGCCAG CAACCCCTGC GACTACGGCA AGTGCATCGA CAAGATCAAC 
GGCTATGAGT GCACCTGTGA GCCGGGGTAC ACAGGGCGCA TGTGCAACAT CAACATCGAT 
GAGTGTGCCA GCAACCCATG CCACAATGGG GGCACATGCA AGGATGGCAT CAACGGCTTC 
ACCTGCCTCT GCCCCGAAGG CTTCCATGAC CCCAAGTGCC TGTCCGAAGT GAATGAGTGC 
AACAGCAACC CCTGCATCCA CGGGAGGTGC CACGACGGGC TGAACGGTTA CCGCTGTGAT 
TGCGACCCGG GCTGGAGCGG GACAAACTGT GACATCAACA ACAACGAGTG TGAATCCAAC 
CCCTGCATGA ATGGTGGCAC CTGCAAGGAC ATGACCAGCG GTTACATCTG CACCTGCAGG 
GAGGGGTTCA GCGGACCCAA CTGCCAGACC AATATCAATG AATGTGCTTC CAACCCCTGC 
CTGAACCAAG GCACGTGCAT CGATGATGTG GCCGGCTACA CCTGCAACTG CCTCCTGCCC 
TACACAGGAG CCACCTGTGA GGACGTGCTG GCCCCCTGCG CCGGCGGCCC CTGCAAGAAC 
GGCGGCGAGT GCCGGGAGTC AGAGGACTAC AAGAGGTTCT CGTGCAGCTG CCCGCCCGGC 
TGGCAAGGCC AGACGTGTGA GATTGACATC AATGAGTGTG TGAAGAGCCC GTGCCGCAAT 
GGGGCCACGT GTCAGAACAC CAACGGGAGC TACCGCTGCC TGTGCCGGGT GGGCTTCGCG 
GGCCGCAACT GCGACACCGA CATCGACGAC TGCCAGCCCA ATCCGTGCCA CAATGGTGGC 
TCCTGCTCGG ATGGCATTGG CACATTCTTC TGTGAGTGCC TGGCCGGCTT CCGTGGGCTC 
AAGTGTGAGG AGGACATCAA TGAGTGTGCC AGCAACCCCT GCAAGAACGG GGCCAACTGC 
ACCGACTGCG TCAACAGCTA CACCTGCACC TGCCCCTCCG GCTTCAGCGG CATCCACTGC 
GAGAACAACA CACCAGACTG CACTGAGAGC TCCTGCTTCA ATGGGGGGAC CTGCGTGGAT 
GGCATCAACA CCTTCACCTG CCTCTGTCCA TCCGGCTTCA CGGGCAGCTA CTGTGAGCAC 
AACATCAACG AGTGTGACTC CAAGCCCTGC CTGAACGGGG GCACGTGTCA GGACAGCTAT 
GGGACGTACA AGTGCACCTG TCCCCAGGGA TACACTGGGC TCAACTGCCA GAACTTGGTG 
CGCTGGTGTG ACTCCTCACC CTGCAAAAAC GGGGGCAAGT GCTGGCAGAC CAACAACCTG 
TACCGCTGCG AGTGCAACAG CGGCTGGACG GGGCTCTACT GTGATGTCCC CAGCGTCTCC 
TGCGAGGTGG CTGCAAAGCA GCAAGGTATC GACGTCGCTC ATCTCTGCAG GAACTCAGGG 
CTCTGTGTGG ACAGTGGCAA CACTCACTTC TGCCGCTGCC AGGCTGGCTA CACCGGCAGC 
TACTGCGAGG AGCAGGTGGA TGAGTGCTCC CCCAACCCCT GCCAGAACGG AGCCACCTGC 
ACCGACTACC TGGGGGGCTA TTCCTGTGAG TGCGTGGCTG GTTATCATGG AGTTAACTGC 
TCAGAGGAGA TCAATGAGTG CTTGTCCCAC CCATGCCAGA ATGGAGGAAC CTGCATCGAT 
CTCATCAATA CCTACAAATG CTCCTGCCCC AGAGGAACTC AAGGGGAGCG CTGCGAGGGA 
GACGTCAACG AGTGCCTCTC CAACCCCTGC GACGCGCGCG GCACCCAGAA CTGCGTGCAG 
CGGGTCAATG ACTACAAATG CGAGTGCCGG CCTGGCTACG CAGGGCGTCG CTGTGACACC 
GTGGTGGATG GCTGCAAGGG CAAACCCTGC AGGAATGGGG GAACGTGCGC TGTGGCCAGC 
AACACCGGCC GCGGCTTCAT CTGCAAGTGC CCCCCGGGCT TTGTGGGTGC CACCTGCGAG 
AACGACTCCC ACACCTGCGG GACCCTGCAC TGCCTGAACG GTGGCACCTG CATCTCCATG 
CACAAGAGCT CCAAGTGTGT GTGCGCAGCG GCCTTCACGG GCCCCGAGTG CCAATACCCG 
GCCAGCAGCC CCTGCATCTC CAACCCCTGC TACAACGGGG GCACCTGCGA GTTCCTCAGC 
GACGCCTCCC CTTACTACCA CTGCAACTGC CCCGCCAACT TCAACGGCCT CAACTGCCAC 
ATCCTCGACT TTGATTTCCA AGGCGGCTTC GGGCAGGACA TCATCCCACC CAAAATCGAG 
GAGAAGTGTG AGATCGCCGT TTGTGCGAGC TATGCTGGCA ATAAGATCTG TGATGGGAAG 
TGCAACAACC ACGCCTGCGG CTGGGACGGC GGCGACTGCT CGCTCAATTT CAACGACCCC 
TGGAAGAACT GCTCACAGTC ACTGCAGTGC TGGAAGTACT TCAATGATGG CAAGTGTGAC 
TCGCAGTGCA ACAACGCGGG CTGCCTGTAT GATGGGTTTG ACTGCCAGAA GTACGAGGGG 
CAGTGCAACC CTCTGTACGA CCAGTACTGT AAGGACCACT TCTCAGATGG TCACTGTGAC 
CAGGGCTGCA ATAACTTTGA GTGTGAGTGG GACGGGCTGG ACTGTGCAAA CAACATGCCC 
GAGAAGCTGG CGGATGGCAC GCTGGTGGTG GTGGTCCTCA TCACCCCTGA GAACCTGAAG 
AACAATTCCT TCAATTTCCT GAGGGAGCTG AGCCGTGTGC TGCACACCAA TGTAGTCTTC 
AAGAAGAACG CCAAGGGAGA GTACATGATC TTCCCATACT ATGGCAACGA GGAGGAGCTG 
AAAAAGCACT ACATCAAGAG GTCAACGGAG GACTGGGCAG ATATGTCCAG TGCTGTCATC 
AACAAGGTGA AGAGCAGCCT CTACTCGCGG GCTGGCAGGA GGCAGAAGAG AGAGCTGGAT 
CAGATGGACA TCAGAGGATC CATCGTCTAC TTGGAAATTG ACAACAGGCA GTGCATCCAG 
TCGTCCTCCC AGTGCTTCCA GAGCGCAACC GATGTGGCGG CCTTCCTGGG GGCCTTGGCC 
TCCCTGGGCA ACCTCAACAT TCCCTACAAA ATAGAGGCCG TCAAGAGTGA AACGGCGGAG 
CCCGCGAGGA ACTCCCAGCT GTATCCTATG TATGTGGTGG TGGCTGCACT GGTCTTGCTT 
GCCTTCATTG GAGTGGGAGT GCTGGTGTCC CGCAAGCGGC GCAGGGAGCA TGGCCAGCTC 
TGGTTCCCAG AGGGCTTCAA AGTGACGGAG TCGAGCAAGA AGAAGCGCCG GGAACCACTT 
GGGGAAGATT CTGTTGGACT GAAACCCCTC AAAAATGCTT CTGACGGCAC GCTGATGGAC 
GACAACCAAA ATGAGTGGGG TGACGAGGAG ACCCTGGACA CCAAGAAGTT CAGGTTCGAG 
GAGCAGGCGA TGCTGCCCGA CACGGATGAC CAGACGGACC ACAGGCAGTG GACTCAGCAG 
CACCTGGATG CTGCCGACCT CCGCATCTCC TCCATGGCCC CTACCCCACC ACAGGGCGAG 
ATCGATGCAG ACTGCATGGA TGTCAACGTA CGAGGTCCAG ACGGCTTCAC GCCCCTCATG 
ATCGCCTCGT GCAGCGGAGG GGGGCTGGAG ACTGGCAACA GCGAGGAGGA GGACGATGCC 
CCTGCAGTCA TCTCAGATTT CATCTACCAA GGCGCCAGTT TGCACAACCA GACTGACCGC 
ACCGGCGAGA CCGCGCTGCA CCTGGCTGCC CGCTACTCGC GCTCCGACGC TGCCAAACGC 
CTGCTGGAAG CCAGTGCTGA TGCGAACATT CAGGATAACA TGGGCAGGAC CCCTCTGCAC 
GCCGCTGTCT CTGCTGATGC CCAAGGAGTC TTCCAGATCC TGATAAGGAA CAGGGCGACT 
GATCTCGATG CCCGAATGCA CGATGGGACG ACCCCACTGA TCCTGGCTGC TCGCTTGGCT 
GTGGAGGGGA TGCTGGAGGA CCTCATCAAC TGCCACGCAG ACGTCAACGC TGTGGATGAT 
CTGGGCAAGT CAGCACTGCA CTGGGCAGCT GCTGTGAACA ATGTCGAAGC CGCTGTCGTC 
CTCCTGAAGA ACGGTGCCAA TAAGGATATG CAGAACAATA AGGAGGAGAC CCCGCTGTTC 
CTCGCCGCCA GAGAAGGGAG CTACGAAACA GCCAAGGTTC TGCTGGACCA CTTTGCGAAC 
CGCGACATCA CGGACCACAT GGACCGGCTG CCGCGGGACA TCGCGCAGGA GCGCATGCAC 
CACGACATCG TGCGGCTGCT GGATGAGTAC AACCTGGTGC GGAGCCCTCC GCTGCACAGC 
GGCCCTCTCG GGGCCCCCAC GCTGTCCCCC CCGCTCTGCT CCCCCAGCAG TTACATCGGC 
AACCTGAAGC CGGCCGTGCA GGGCAAGAAG GCCAGGAAGC CGAGCACCAA GGGCCTGAGC 
TGCAACGGCA AGGATTCCAA AGACCTGAAA GCCCGGAGGA AAAAATCACA AGATGGAAAA 
GGCTGTCTGC TCGACAACTC CAGCGTGCTG TCCCCAGTGG ACTCCCTGGA GTCACCCCAC 
GGGTACCTGT CCGACGTCGC CTCCCCTCCG CTGATGACTT CTCCGTTCCA GCAGTCCCCT 
TCCATGCCTC TGAACCACCT GCCTGGCATG CCCGACGCCC ACATGAGCAT CAACCACCTC 
AACATGGCAG GGAAGCAGGA GATGGCCCTG GGCGGCTCTG GCAGGATGGC CTTCGAGGCG 
GTGCCGCCGC GCCTCTCGCA CCTCCCCGTC TCCAGCCCCA GCACGGCGAT GAGCAACGCC 
CCGATGAATT TCTCCGTCGG CGGAGCTGCC GGCCTGAGCG GGCAGTGCGA CTGGCTGAGC 
CGGCTGCAGA GCGGCATGGT GCAGAACCAG TACGGCGCCA TGCGGGGCGG CATGCAGCCG 
GGCACGCACC AGCAAGCACA GAACCTGCAG CACGGCATGA TGAGCTCCCT GCACAACGGG 
CTGCCCAGCA CCAGCCTGTC GCAGATGATG AGCTACCAGG CCATGCCCAG CACCCGGCTG 
GCCTCCCAGC CCCACCTGCT GCAGAACCAG CAGATGCAGC AGATGCAGCA GCCCGGAATG 
CAGCCGCAGC CCGGAATGCA GCCGCAGCCC GGCATGCAGC AGCCTCAACA GCAGCCCCAG 
CAACAGCCCC AGCCGCAGCA GCACCACAAC CCCGGCTCCA ACGCCAGCGG CCACATGGGC 
CAAAATTTCC TCGGTACTGA GCTGAGCCAG CCCGACATGC AGCCGGTGAG CAGCAGCGCC 
ATGGCGGTGC ACACCATCCT GCCCCAAGAT TCGCAGCTGC TACCCACCTC TCTGCCGTCC 
TCCCTCGCGC AGCCCATGAC CACCACGCAG TTCCTGACCC CCCCTTCCCA GCACAGCTAT 
TCCTCCCCGT TGGACAACAC CCCCAGCCAC CAGCTCCAGG TGCCCGACCA CCCCTTCCTC 
ACTCCCTCTC CGGAGTCTCC AGACCAGTGG TCGAGCTCCT CGCCCCACTC CAACGTGTCC 
GACTGGTCCG AGGGCATCTC CAGCCCCCCC ACCAGCATGC AGTCGCAGAT GGGACACATC 
CCCGAGGCCT TCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis.
eLife5(2016 08)
Esteves de Lima J,Bonnin MA,Birchmeier C,Duprez D


book

Cytoplasmic NOTCH and membrane-derived ?-catenin link cell fate choice to epithelial-mesenchymal transition during myogenesis.
eLife5(2016 05)
Sieiro D,Rios AC,Hirst CE,Marcelle C


book

A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo.
Development (Cambridge, England)142(13)2291-303(2015 Jul)
Stasiulewicz M,Gray SD,Mastromina I,Silva JC,Bj?rklund M,Seymour PA,Booth D,Thompson C,Green RJ,Hall EA,Serup P,Dale JK


book

Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
Development (Cambridge, England)141(8)1671-82(2014 Apr)
Hatakeyama J,Wakamatsu Y,Nagafuchi A,Kageyama R,Shigemoto R,Shimamura K