COL27A1 cDNA ORF clone, Gallus gallus(chicken)

  • Gene

  • Clones

  • gRNAs

The following COL27A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL27A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa43621 XM_015279700.1
Latest version!
Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa145169 XM_025141345.1
Latest version!
Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa145170 XM_025141347.1
Latest version!
Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa145170 XM_025141346.1
Latest version!
Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa145171 XM_025141348.1
Latest version!
Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa43620 XM_015279698.1
Latest version!
Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa43620 XM_015279699.2
Latest version!
Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa16108 XM_415514.5
Latest version!
Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OGa43620 XM_015279699.1 Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa43621
    Clone ID Related Accession (Same CDS sequence) XM_015279700.1
    Accession Version XM_015279700.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG ATACCACAGA GTACGGTTCA GGTCAGGGCT
    TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135186.1
    CDS488..4933
    Translation

    Target ORF information:

    RefSeq Version XM_015279700.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279700.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG ATACCACAGA GTACGGTTCA GGTCAGGGCT
    TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa145169
    Clone ID Related Accession (Same CDS sequence) XM_025141345.1
    Accession Version XM_025141345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAATATG GCTACAGGAA GACAAGAAGT AGACGTTGTT GTAAGCTGCT GGGACAAATG 
    AATGCCAACG AAGGTGGGGC TGCAAGGAGA CGTTGCACAG CCTCTCGCCT CGCTGCAAAG
    GGATTCTGCT TTGTCTGGAC TGTGTTGTGT TTTAGCTGGT TCCTGGGATT CACACAGGGA
    AACTCTGAAG ATGTGGATGT TCTTCAGAGG CTGGGCCTGG GAGGGAGAAG GCCATCGAGC
    GGATTGTCCT CATCACATGC CATTCCTCAA GGGGTCATTC CTCTCAAATC AGGGGTTATC
    TTTACTCAGC GAGCACGCGT CGAAGCACCC ATCAGCACCA TCTTCCCCGC AGGCTCCAGC
    ACTGACCTGC TCCTAGTGCT GAGCCTGTGC TCCCACCGCA TCAACAATGC TTTCCTCTTT
    GCAGTCAAGA GCAAAAAGAA AAAACTGCAG CTGGGGGTTC AGTTTGTGCC TGGGAAGATA
    ATAGTGTACG TGGGCCACAA GCACTCTGTA TATTTTGATT ACAATGTTCA CGATGGCCAG
    TGGCACAATA TGGCCATCAA TATCCGTGGC CAGACAGTCA CTTTATTTAC TTCTTGTGGG
    AAGCACAGAG TACATGCGGA TTTGCATTTT AAAAAGGACG AGGCATTGGA TCCACATGGA
    TCTTTCCTCT TTGGGAAGAT GAACCATCAC TCCGTGCAGT TTGAAGGAGC CATCTGCCAG
    TTCGATATTT ATCCTTCCGC CAAGGCAGCT CATCATTACT GTAAATACCT GAAAAAACAG
    TGCAGGCAAG CAGACACCTA CCGGCCAAAC CTGCCCCCAC TCATCCCCAT CCTGCCCAGG
    GATCCCAGTG CCTCCCCGAG TACTCCACAG CATATGGAGC CCTCGCTTCA GGGTCTGAAA
    AACTTGACCA CTGCCTCCCC ATCCTTGGCG CTTGGCAGGG TCACCGCACC CCTCAAGACT
    CGGGGTTCAG GTGCTACAAC TGTTGCCTTG GTCTCGTCCC AGCCGCCGCT GCCAAGACAG
    ACAACCAAAG CCACAAAGGT TACAGTGGCC CCATCTCCCG CTCCATCGAG TGCTGGGACG
    CAGCCGCCCA CCCGTTCGCT GCCTCGGGGG ACGGTGACGA CCCTCAGGGT GTCTCGGCCC
    ACTTCCAGCA CACACACGGA GAAAACTCCC CTGCCTGGTG CCAAAAAGGC CCTGCCAGCA
    AGCCCGAGCC CTGCCACGGC CAGCAGGAAA GAGACCAAGC AAGAGACCAA AAAGAAGATC
    AACCAAATAC CAACCACCAA GTCCTCTGAA GCACCCAGTA CTGTAAAGCC TACCAGGAGG
    AGGGCTGACA ACGCAACCAG CAGCGTCCAC TTTGTCACCC TGAAGCAAAC CCCACAGACC
    CCAACCACCG GGCCCCTTCC AAAGAAGCAC CTGCCAACCC CCACCAGGAA AGGGGATCCT
    GGCAATGTCC CTCTGGTGTA CACCAAACCG CCCCTGGGGG CTGCCCGTCC TGTCTCCAGA
    GCCCAGGCCT TCAACCTCAC CACCCCAGCT GCGACCGATG GCTATCAGAT CTTTGACCCC
    CTTGGGCCCA CTCCGTTTCC ATTTTTACTG GGATACCCAG GAGTGAAAGG AGACAGAGGA
    CCTCAGGGTC CACCAGGTCT GCCGGGCTTA CCAGGACCAC CAGGCAAGAG AGGTTCCCGA
    GGTCCTCCGG GTCCCCATGG AAACCCTGGT TCACCTGGCC CCCCAGGCCT GAAAGGTCAG
    AAAGGTGATC CTGGGCTGTC GCCTGGAAAA GCTCGCAGAG GACCAAAGGG AGACGTGGGC
    TTACCTGGTC TGACTGGGTT CCCTGGGCCA CCTGGTAGAA AGGGATACAA AGGCTATCCT
    GGACCACCGG GTCACCCTGG AGACCAGGGT GAACGAGGAC CAGCCGGCAG CCCTGGTGCC
    AAAGGTTATC CTGGCAGGCA GGGCTTACCT GGCCCCATAG GGGAAGCTGG TCCAAAAGGC
    AGTCGGGGAT ATATTGGTCT TCCGGGATTA TTTGGGCTAC CAGGGGCTGA TGGAGAACGA
    GGGATCCCTG GAGTTCCTGG GAAGAGGGGC AAGATGGGTA GGCCGGGTTT TCCTGGTGAT
    TTTGGGGAAC GAGGACCTCC AGGCATCGAC GGGAACCCAG GAGAATTGGG AGCCCCAGGT
    CCTCCTGGAG TCCTTGGACT CATTGGTGAC ATGGGCTCTG TTGGACCTAT TGGTTATCCA
    GGACCAAAGG GCTTAAAGGG GCTGATAGGA AACCCTGGAG AACATGGACT GAAAGGTGAT
    AAGGGTGATC AGGGAGGAGC AGGTCTTCCA GGAGATATTG GCTTCCAAGG AGACAAGGGT
    AGCCACGGTT TGCCTGGGGT CCCTGGGGCA CGAGGGAAAC CAGGCCCTGT GGGAAAAGTT
    GGTGACATAG GTCCGATGGG GTTTCCAGGA CCACCTGGCC CTGAGGGCTT CCCAGGCGAC
    ATCGGCCCGC CGGGAGAGAA CGGCCCCGAA GGCCCAAAGG GGAAGCCGGG AGCGCGGGGT
    TTGCCTGGGC CCAGAGGGCT GCCTGGACAA GAGGGAGATG AAGGACCCTT AGGACCACCA
    GGAGTCCCTG GTTCAGAGGG TCGACCTGGC AGGAAGGGAT TTCCGGGAAG ACCTGGTCCT
    GACGGTCCCA AGGGCGAGCC AGGAAACCAC GGCCGGCCGG GGAAAGTTGG TGAGCAGGGT
    CTAATGGGAT TCATTGGTCC AGTAGGGGAG CCTGGAATAG TGGGAGAAAA GGGTGACCGT
    GGCTCAGTGG GACCCCCAGG CCCTCCTGGA GTCAAGGGGT CGATGGGGCA CCCTGGGATA
    CCAGGAGCAG TGGGTTTTCC CGGCATCCCC GGACCAGCAG GTCCGGCAGG AGCCCGCGGT
    GCACCAGGGA TCAGAGGGGT GAAAGGCCGC CGGGGTGCCA GAGGGCCTGA TGGACCAGTA
    GGCGAGCCGG GGTCACGAGG TGCCAAGGGC CGTCCAGGGG AGCCAGGCAA GCTGGGGCCA
    GATGGGCTGC AGGGGAAGAT GGGCGAGACT GGGGAGACAG GATCCCGTGG CTTTCCAGGT
    ATCCAAGGGC CTTCTGGACC TCCAGGAACA AAAGGGGCAC CAGGCGACCC GGGTCCACAA
    GGACCACAGG GACCACCTGG ACCTTTGGGA GAAATTGGCC ATAAGGGACC GCCTGGCCCA
    ATGGGACAAC CTGGCCTCGC AGGTGAACCT GGCATGAAGG GTGATCCTGG CCAGCTGGGA
    GTTCCTGGAG AGCAAGGCCT CATTGGTCAA AGAGGAGAAA TGGGGCTGGA AGGGGACAGT
    GGGCCACCTG GTCCAGACGG AGCCAAGGGA GACAAAGGAG AGCAAGGCCT GGATGGAGAG
    AAAGGAGAAA AAGGCACTGA AGGACTGAAG GGAAAAGAAG GGCCTCCTGG ATATCCCGGC
    ATCACAGGTG TCCGAGGACC AGAAGGGAAA CCAGGAAAAC AGGGGGAAAA GGGCAGGCCT
    GGCCAGAAGG GCAGCAAGGG CTACCAGGGA CAGCTTGGCG AGACAGGACC TCCAGGGAAG
    GAGGGGCCAA AGGGGAACCA AGGCCCTAAA GGATCCCGAG GTACCCTGGG ACCTATGGGT
    GCTCCAGGAA GGATGGGTGC TCAGGGGGAG CCTGGCTTGA AGGGTTACCA GGGGCACGCA
    GGACCACCAG GGCCAATTGG ACCACCGGGG CCGAAGGGTG AGAAGGGAGA ACAAGGTGAT
    GACGGGAAGG TGGAAGGCCC ACAGGGACCA CCCGGAGACA GAGGTCCTGT TGGTGACAGA
    GGAGATCGAG GGGAGCCCGG AGACCCTGGT TATCCTGGCC AAGAAGGGCT TGATGGAGAA
    AGAGGAAAAC CTGGACAGCA AGGCTTACCT GGGGATCCTG GACCACCAGG CCAGCCAGGA
    GCAAAGGGAT CCAAAGGTGA TGTGGGTCAA AAAGGAAAGC AAGGAGCACG AGGCAGTGCA
    GGGAGCAGAG GCTCACCGGG CACCCTTGGG CTACCTGGTC CTCGAGGAGT GGTGGGAAGG
    CAGGGCCAAG AGGGTGCTCC TGGAGTGGAT GGAGTGCCTG GGAAGGACGG CGTGCCTGGG
    GTGCTGGGAG AGCAGGGAGA TGATGGGGAA GAAGGAGTAA CAGGGATGAC AGGCAAAAGA
    GGGAACCCCG GCGTACCGGG ACTGCCCGGG GCACAGGGAC CACCAGGATT CAAGGGTGAA
    AGAGGATTGC CTGGACAGAC TGGCCAAACA GGGAAGCGAG GATCACTGGG AGGAATGGGC
    CTCCCAGGGA GCCCCGGCGA TCCAGGACCC AAAGGCCAGC CGGGAGACTT TGGGGAGGCA
    GGATTCCCAG GGATCGCGGG CATCTTTGGG CCAAAGGGAC CTCCAGGAGA CCCCGGTTTC
    AAAGGCATTC AGGGACCACG TGGCCCACCT GGCATCATGG GAAAAGAAGG AATTATTGGT
    CCACTTGGCA TCCTGGGTCC CACAGGAAGC CCGGGGCCAA AGGGGGACAA AGGCAGCCGC
    GGGGATGTGG GGCTTCGGGG CCCAAGGGGT CCTCCAGGCC CACGAGGACA GCCTGGCCCT
    CCGGGACCGC CGGGATTTCC AGCTTCATTT CAACAAGATG GCTTTGGGGC TGCTTTCCGG
    ACACTGGTTG ACTCCAACGT GGCACTCAAG ACAGAGGGTT ACCAACATCC AGACCTTCTC
    ATGCTGGACC ATGGAGGGGA GATCTTTAAG ACTCTACACT ACCTCAGCAA CCTCATTCAG
    AGCATCAAGA GACCCCTGGG CACCAAGGAA AACCCTGCAC GCATCTGCCG AGACCTCATG
    AACTGTGAAC AGAAGATGAA GGATGGTACC TACTGGATCG ACCCAAATCT CGGCTGTTCC
    TCAGACACCA TACAAGTCAT CTGCAACTTC ACCAACGGTG GGCAGACGTG TCTCAGCCCC
    ATCACCGTCT CCAAGCTGGA TTTTGACATC GGCAGAGTCC AGATGAACTT CCTGCGCCTG
    CTGAGCTCGG AGGTGGTGCA GCACATCACC ATCCACTGCC TGAACACCTC TGTCTGGAGG
    GAGGGCTCTG CTGAGAAACC TTCACAGAAA GCCGTGCGCT TCAAGGCGTG GAACGGGCAA
    ATGTTTGAGG CAGAGGGGCA GCTCAGGCCG GAGGTGGCAG CTGATGATTG CAAGATCAAG
    GATGGACGCT GGCATCGGAC TCTCTTTTCC TTTCGGACGC AGGACACCCA GCAGCTGCCA
    ATCATCAACA TCTACAACCT CCCCCCATCC GAGCCAGGAA AGCATTATCA CCTCGAGGGT
    CTCTGCAGCA AGGACAGCTG GGCTGTGTCT TTTGGGTCAA CTCTTGCTCT AGCCTTTGGC
    TCAAGGTCTC AAAGAACCCG TGATCTACTG AACTCAGCAG GTTGTTGGAA GGAGGAATAC
    GAAGGACGTA GGGGTAGCGG GTGTCGGTTG GGACGTGACC AGTGCAAAGG GATCAGGGGA
    CGAGGTGTCG CACGCGGCTC CACACACAAA GCTTTTTCCA TGACCAAAGA AGACAAGAAT
    GAGAGAAACC TCAAGCGACT CGCAGCATGT GGTTTCCCCA CCCTCCCCCC CTATTTTTGC
    CTTACCCGGT TCCCGAGTTA CCAAATGTTC TTCCCATTGA GCAGTAATTC AGAGATGATA
    GGGCTTCCCT CCTGGATGGC CGCGCTGCGC CGGGCTCTCC CAGGCGGAGC TGCAATGCGA
    CGCCGGCTGC TCTGCGCACC GGAACCATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997113.1
    CDS16..5748
    Translation

    Target ORF information:

    RefSeq Version XM_025141345.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025141345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAATATG GCTACAGGAA GACAAGAAGT AGACGTTGTT GTAAGCTGCT GGGACAAATG 
    AATGCCAACG AAGGTGGGGC TGCAAGGAGA CGTTGCACAG CCTCTCGCCT CGCTGCAAAG
    GGATTCTGCT TTGTCTGGAC TGTGTTGTGT TTTAGCTGGT TCCTGGGATT CACACAGGGA
    AACTCTGAAG ATGTGGATGT TCTTCAGAGG CTGGGCCTGG GAGGGAGAAG GCCATCGAGC
    GGATTGTCCT CATCACATGC CATTCCTCAA GGGGTCATTC CTCTCAAATC AGGGGTTATC
    TTTACTCAGC GAGCACGCGT CGAAGCACCC ATCAGCACCA TCTTCCCCGC AGGCTCCAGC
    ACTGACCTGC TCCTAGTGCT GAGCCTGTGC TCCCACCGCA TCAACAATGC TTTCCTCTTT
    GCAGTCAAGA GCAAAAAGAA AAAACTGCAG CTGGGGGTTC AGTTTGTGCC TGGGAAGATA
    ATAGTGTACG TGGGCCACAA GCACTCTGTA TATTTTGATT ACAATGTTCA CGATGGCCAG
    TGGCACAATA TGGCCATCAA TATCCGTGGC CAGACAGTCA CTTTATTTAC TTCTTGTGGG
    AAGCACAGAG TACATGCGGA TTTGCATTTT AAAAAGGACG AGGCATTGGA TCCACATGGA
    TCTTTCCTCT TTGGGAAGAT GAACCATCAC TCCGTGCAGT TTGAAGGAGC CATCTGCCAG
    TTCGATATTT ATCCTTCCGC CAAGGCAGCT CATCATTACT GTAAATACCT GAAAAAACAG
    TGCAGGCAAG CAGACACCTA CCGGCCAAAC CTGCCCCCAC TCATCCCCAT CCTGCCCAGG
    GATCCCAGTG CCTCCCCGAG TACTCCACAG CATATGGAGC CCTCGCTTCA GGGTCTGAAA
    AACTTGACCA CTGCCTCCCC ATCCTTGGCG CTTGGCAGGG TCACCGCACC CCTCAAGACT
    CGGGGTTCAG GTGCTACAAC TGTTGCCTTG GTCTCGTCCC AGCCGCCGCT GCCAAGACAG
    ACAACCAAAG CCACAAAGGT TACAGTGGCC CCATCTCCCG CTCCATCGAG TGCTGGGACG
    CAGCCGCCCA CCCGTTCGCT GCCTCGGGGG ACGGTGACGA CCCTCAGGGT GTCTCGGCCC
    ACTTCCAGCA CACACACGGA GAAAACTCCC CTGCCTGGTG CCAAAAAGGC CCTGCCAGCA
    AGCCCGAGCC CTGCCACGGC CAGCAGGAAA GAGACCAAGC AAGAGACCAA AAAGAAGATC
    AACCAAATAC CAACCACCAA GTCCTCTGAA GCACCCAGTA CTGTAAAGCC TACCAGGAGG
    AGGGCTGACA ACGCAACCAG CAGCGTCCAC TTTGTCACCC TGAAGCAAAC CCCACAGACC
    CCAACCACCG GGCCCCTTCC AAAGAAGCAC CTGCCAACCC CCACCAGGAA AGGGGATCCT
    GGCAATGTCC CTCTGGTGTA CACCAAACCG CCCCTGGGGG CTGCCCGTCC TGTCTCCAGA
    GCCCAGGCCT TCAACCTCAC CACCCCAGCT GCGACCGATG GCTATCAGAT CTTTGACCCC
    CTTGGGCCCA CTCCGTTTCC ATTTTTACTG GGATACCCAG GAGTGAAAGG AGACAGAGGA
    CCTCAGGGTC CACCAGGTCT GCCGGGCTTA CCAGGACCAC CAGGCAAGAG AGGTTCCCGA
    GGTCCTCCGG GTCCCCATGG AAACCCTGGT TCACCTGGCC CCCCAGGCCT GAAAGGTCAG
    AAAGGTGATC CTGGGCTGTC GCCTGGAAAA GCTCGCAGAG GACCAAAGGG AGACGTGGGC
    TTACCTGGTC TGACTGGGTT CCCTGGGCCA CCTGGTAGAA AGGGATACAA AGGCTATCCT
    GGACCACCGG GTCACCCTGG AGACCAGGGT GAACGAGGAC CAGCCGGCAG CCCTGGTGCC
    AAAGGTTATC CTGGCAGGCA GGGCTTACCT GGCCCCATAG GGGAAGCTGG TCCAAAAGGC
    AGTCGGGGAT ATATTGGTCT TCCGGGATTA TTTGGGCTAC CAGGGGCTGA TGGAGAACGA
    GGGATCCCTG GAGTTCCTGG GAAGAGGGGC AAGATGGGTA GGCCGGGTTT TCCTGGTGAT
    TTTGGGGAAC GAGGACCTCC AGGCATCGAC GGGAACCCAG GAGAATTGGG AGCCCCAGGT
    CCTCCTGGAG TCCTTGGACT CATTGGTGAC ATGGGCTCTG TTGGACCTAT TGGTTATCCA
    GGACCAAAGG GCTTAAAGGG GCTGATAGGA AACCCTGGAG AACATGGACT GAAAGGTGAT
    AAGGGTGATC AGGGAGGAGC AGGTCTTCCA GGAGATATTG GCTTCCAAGG AGACAAGGGT
    AGCCACGGTT TGCCTGGGGT CCCTGGGGCA CGAGGGAAAC CAGGCCCTGT GGGAAAAGTT
    GGTGACATAG GTCCGATGGG GTTTCCAGGA CCACCTGGCC CTGAGGGCTT CCCAGGCGAC
    ATCGGCCCGC CGGGAGAGAA CGGCCCCGAA GGCCCAAAGG GGAAGCCGGG AGCGCGGGGT
    TTGCCTGGGC CCAGAGGGCT GCCTGGACAA GAGGGAGATG AAGGACCCTT AGGACCACCA
    GGAGTCCCTG GTTCAGAGGG TCGACCTGGC AGGAAGGGAT TTCCGGGAAG ACCTGGTCCT
    GACGGTCCCA AGGGCGAGCC AGGAAACCAC GGCCGGCCGG GGAAAGTTGG TGAGCAGGGT
    CTAATGGGAT TCATTGGTCC AGTAGGGGAG CCTGGAATAG TGGGAGAAAA GGGTGACCGT
    GGCTCAGTGG GACCCCCAGG CCCTCCTGGA GTCAAGGGGT CGATGGGGCA CCCTGGGATA
    CCAGGAGCAG TGGGTTTTCC CGGCATCCCC GGACCAGCAG GTCCGGCAGG AGCCCGCGGT
    GCACCAGGGA TCAGAGGGGT GAAAGGCCGC CGGGGTGCCA GAGGGCCTGA TGGACCAGTA
    GGCGAGCCGG GGTCACGAGG TGCCAAGGGC CGTCCAGGGG AGCCAGGCAA GCTGGGGCCA
    GATGGGCTGC AGGGGAAGAT GGGCGAGACT GGGGAGACAG GATCCCGTGG CTTTCCAGGT
    ATCCAAGGGC CTTCTGGACC TCCAGGAACA AAAGGGGCAC CAGGCGACCC GGGTCCACAA
    GGACCACAGG GACCACCTGG ACCTTTGGGA GAAATTGGCC ATAAGGGACC GCCTGGCCCA
    ATGGGACAAC CTGGCCTCGC AGGTGAACCT GGCATGAAGG GTGATCCTGG CCAGCTGGGA
    GTTCCTGGAG AGCAAGGCCT CATTGGTCAA AGAGGAGAAA TGGGGCTGGA AGGGGACAGT
    GGGCCACCTG GTCCAGACGG AGCCAAGGGA GACAAAGGAG AGCAAGGCCT GGATGGAGAG
    AAAGGAGAAA AAGGCACTGA AGGACTGAAG GGAAAAGAAG GGCCTCCTGG ATATCCCGGC
    ATCACAGGTG TCCGAGGACC AGAAGGGAAA CCAGGAAAAC AGGGGGAAAA GGGCAGGCCT
    GGCCAGAAGG GCAGCAAGGG CTACCAGGGA CAGCTTGGCG AGACAGGACC TCCAGGGAAG
    GAGGGGCCAA AGGGGAACCA AGGCCCTAAA GGATCCCGAG GTACCCTGGG ACCTATGGGT
    GCTCCAGGAA GGATGGGTGC TCAGGGGGAG CCTGGCTTGA AGGGTTACCA GGGGCACGCA
    GGACCACCAG GGCCAATTGG ACCACCGGGG CCGAAGGGTG AGAAGGGAGA ACAAGGTGAT
    GACGGGAAGG TGGAAGGCCC ACAGGGACCA CCCGGAGACA GAGGTCCTGT TGGTGACAGA
    GGAGATCGAG GGGAGCCCGG AGACCCTGGT TATCCTGGCC AAGAAGGGCT TGATGGAGAA
    AGAGGAAAAC CTGGACAGCA AGGCTTACCT GGGGATCCTG GACCACCAGG CCAGCCAGGA
    GCAAAGGGAT CCAAAGGTGA TGTGGGTCAA AAAGGAAAGC AAGGAGCACG AGGCAGTGCA
    GGGAGCAGAG GCTCACCGGG CACCCTTGGG CTACCTGGTC CTCGAGGAGT GGTGGGAAGG
    CAGGGCCAAG AGGGTGCTCC TGGAGTGGAT GGAGTGCCTG GGAAGGACGG CGTGCCTGGG
    GTGCTGGGAG AGCAGGGAGA TGATGGGGAA GAAGGAGTAA CAGGGATGAC AGGCAAAAGA
    GGGAACCCCG GCGTACCGGG ACTGCCCGGG GCACAGGGAC CACCAGGATT CAAGGGTGAA
    AGAGGATTGC CTGGACAGAC TGGCCAAACA GGGAAGCGAG GATCACTGGG AGGAATGGGC
    CTCCCAGGGA GCCCCGGCGA TCCAGGACCC AAAGGCCAGC CGGGAGACTT TGGGGAGGCA
    GGATTCCCAG GGATCGCGGG CATCTTTGGG CCAAAGGGAC CTCCAGGAGA CCCCGGTTTC
    AAAGGCATTC AGGGACCACG TGGCCCACCT GGCATCATGG GAAAAGAAGG AATTATTGGT
    CCACTTGGCA TCCTGGGTCC CACAGGAAGC CCGGGGCCAA AGGGGGACAA AGGCAGCCGC
    GGGGATGTGG GGCTTCGGGG CCCAAGGGGT CCTCCAGGCC CACGAGGACA GCCTGGCCCT
    CCGGGACCGC CGGGATTTCC AGCTTCATTT CAACAAGATG GCTTTGGGGC TGCTTTCCGG
    ACACTGGTTG ACTCCAACGT GGCACTCAAG ACAGAGGGTT ACCAACATCC AGACCTTCTC
    ATGCTGGACC ATGGAGGGGA GATCTTTAAG ACTCTACACT ACCTCAGCAA CCTCATTCAG
    AGCATCAAGA GACCCCTGGG CACCAAGGAA AACCCTGCAC GCATCTGCCG AGACCTCATG
    AACTGTGAAC AGAAGATGAA GGATGGTACC TACTGGATCG ACCCAAATCT CGGCTGTTCC
    TCAGACACCA TACAAGTCAT CTGCAACTTC ACCAACGGTG GGCAGACGTG TCTCAGCCCC
    ATCACCGTCT CCAAGCTGGA TTTTGACATC GGCAGAGTCC AGATGAACTT CCTGCGCCTG
    CTGAGCTCGG AGGTGGTGCA GCACATCACC ATCCACTGCC TGAACACCTC TGTCTGGAGG
    GAGGGCTCTG CTGAGAAACC TTCACAGAAA GCCGTGCGCT TCAAGGCGTG GAACGGGCAA
    ATGTTTGAGG CAGAGGGGCA GCTCAGGCCG GAGGTGGCAG CTGATGATTG CAAGATCAAG
    GATGGACGCT GGCATCGGAC TCTCTTTTCC TTTCGGACGC AGGACACCCA GCAGCTGCCA
    ATCATCAACA TCTACAACCT CCCCCCATCC GAGCCAGGAA AGCATTATCA CCTCGAGGGT
    CTCTGCAGCA AGGACAGCTG GGCTGTGTCT TTTGGGTCAA CTCTTGCTCT AGCCTTTGGC
    TCAAGGTCTC AAAGAACCCG TGATCTACTG AACTCAGCAG GTTGTTGGAA GGAGGAATAC
    GAAGGACGTA GGGGTAGCGG GTGTCGGTTG GGACGTGACC AGTGCAAAGG GATCAGGGGA
    CGAGGTGTCG CACGCGGCTC CACACACAAA GCTTTTTCCA TGACCAAAGA AGACAAGAAT
    GAGAGAAACC TCAAGCGACT CGCAGCATGT GGTTTCCCCA CCCTCCCCCC CTATTTTTGC
    CTTACCCGGT TCCCGAGTTA CCAAATGTTC TTCCCATTGA GCAGTAATTC AGAGATGATA
    GGGCTTCCCT CCTGGATGGC CGCGCTGCGC CGGGCTCTCC CAGGCGGAGC TGCAATGCGA
    CGCCGGCTGC TCTGCGCACC GGAACCATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa145170
    Clone ID Related Accession (Same CDS sequence) XM_025141346.1 , XM_025141347.1
    Accession Version XM_025141346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAATGCCA ACGAAGGTGG GGCTGCAAGG AGACGTTGCA CAGCCTCTCG CCTCGCTGCA 
    AAGGGATTCT GCTTTGTCTG GACTGTGTTG TGTTTTAGCT GGTTCCTGGG ATTCACACAG
    GGAAACTCTG AAGATGTGGA TGTTCTTCAG AGGCTGGGCC TGGGAGGGAG AAGGCCATCG
    AGCGGATTGT CCTCATCACA TGCCATTCCT CAAGGGGTCA TTCCTCTCAA ATCAGGGGTT
    ATCTTTACTC AGCGAGCACG CGTCGAAGCA CCCATCAGCA CCATCTTCCC CGCAGGCTCC
    AGCACTGACC TGCTCCTAGT GCTGAGCCTG TGCTCCCACC GCATCAACAA TGCTTTCCTC
    TTTGCAGTCA AGAGCAAAAA GAAAAAACTG CAGCTGGGGG TTCAGTTTGT GCCTGGGAAG
    ATAATAGTGT ACGTGGGCCA CAAGCACTCT GTATATTTTG ATTACAATGT TCACGATGGC
    CAGTGGCACA ATATGGCCAT CAATATCCGT GGCCAGACAG TCACTTTATT TACTTCTTGT
    GGGAAGCACA GAGTACATGC GGATTTGCAT TTTAAAAAGG ACGAGGCATT GGATCCACAT
    GGATCTTTCC TCTTTGGGAA GATGAACCAT CACTCCGTGC AGTTTGAAGG AGCCATCTGC
    CAGTTCGATA TTTATCCTTC CGCCAAGGCA GCTCATCATT ACTGTAAATA CCTGAAAAAA
    CAGTGCAGGC AAGCAGACAC CTACCGGCCA AACCTGCCCC CACTCATCCC CATCCTGCCC
    AGGGATCCCA GTGCCTCCCC GAGTACTCCA CAGCATATGG AGCCCTCGCT TCAGGGTCTG
    AAAAACTTGA CCACTGCCTC CCCATCCTTG GCGCTTGGCA GGGTCACCGC ACCCCTCAAG
    ACTCGGGGTT CAGGTGCTAC AACTGTTGCC TTGGTCTCGT CCCAGCCGCC GCTGCCAAGA
    CAGACAACCA AAGCCACAAA GGTTACAGTG GCCCCATCTC CCGCTCCATC GAGTGCTGGG
    ACGCAGCCGC CCACCCGTTC GCTGCCTCGG GGGACGGTGA CGACCCTCAG GGTGTCTCGG
    CCCACTTCCA GCACACACAC GGAGAAAACT CCCCTGCCTG GTGCCAAAAA GGCCCTGCCA
    GCAAGCCCGA GCCCTGCCAC GGCCAGCAGG AAAGAGACCA AGCAAGAGAC CAAAAAGAAG
    ATCAACCAAA TACCAACCAC CAAGTCCTCT GAAGCACCCA GTACTGTAAA GCCTACCAGG
    AGGAGGGCTG ACAACGCAAC CAGCAGCGTC CACTTTGTCA CCCTGAAGCA AACCCCACAG
    ACCCCAACCA CCGGGCCCCT TCCAAAGAAG CACCTGCCAA CCCCCACCAG GAAAGGGGAT
    CCTGGCAATG TCCCTCTGGT GTACACCAAA CCGCCCCTGG GGGCTGCCCG TCCTGTCTCC
    AGAGCCCAGG CCTTCAACCT CACCACCCCA GCTGCGACCG ATGGCTATCA GATCTTTGAC
    CCCCTTGGGC CCACTCCGTT TCCATTTTTA CTGGGATACC CAGGAGTGAA AGGAGACAGA
    GGACCTCAGG GTCCACCAGG TCTGCCGGGC TTACCAGGAC CACCAGGCAA GAGAGGTTCC
    CGAGGTCCTC CGGGTCCCCA TGGAAACCCT GGTTCACCTG GCCCCCCAGG CCTGAAAGGT
    CAGAAAGGTG ATCCTGGGCT GTCGCCTGGA AAAGCTCGCA GAGGACCAAA GGGAGACGTG
    GGCTTACCTG GTCTGACTGG GTTCCCTGGG CCACCTGGTA GAAAGGGATA CAAAGGCTAT
    CCTGGACCAC CGGGTCACCC TGGAGACCAG GGTGAACGAG GACCAGCCGG CAGCCCTGGT
    GCCAAAGGTT ATCCTGGCAG GCAGGGCTTA CCTGGCCCCA TAGGGGAAGC TGGTCCAAAA
    GGCAGTCGGG GATATATTGG TCTTCCGGGA TTATTTGGGC TACCAGGGGC TGATGGAGAA
    CGAGGGATCC CTGGAGTTCC TGGGAAGAGG GGCAAGATGG GTAGGCCGGG TTTTCCTGGT
    GATTTTGGGG AACGAGGACC TCCAGGCATC GACGGGAACC CAGGAGAATT GGGAGCCCCA
    GGTCCTCCTG GAGTCCTTGG ACTCATTGGT GACATGGGCT CTGTTGGACC TATTGGTTAT
    CCAGGACCAA AGGGCTTAAA GGGGCTGATA GGAAACCCTG GAGAACATGG ACTGAAAGGT
    GATAAGGGTG ATCAGGGAGG AGCAGGTCTT CCAGGAGATA TTGGCTTCCA AGGAGACAAG
    GGTAGCCACG GTTTGCCTGG GGTCCCTGGG GCACGAGGGA AACCAGGCCC TGTGGGAAAA
    GTTGGTGACA TAGGTCCGAT GGGGTTTCCA GGACCACCTG GCCCTGAGGG CTTCCCAGGC
    GACATCGGCC CGCCGGGAGA GAACGGCCCC GAAGGCCCAA AGGGGAAGCC GGGAGCGCGG
    GGTTTGCCTG GGCCCAGAGG GCTGCCTGGA CAAGAGGGAG ATGAAGGACC CTTAGGACCA
    CCAGGAGTCC CTGGTTCAGA GGGTCGACCT GGCAGGAAGG GATTTCCGGG AAGACCTGGT
    CCTGACGGTC CCAAGGGCGA GCCAGGAAAC CACGGCCGGC CGGGGAAAGT TGGTGAGCAG
    GGTCTAATGG GATTCATTGG TCCAGTAGGG GAGCCTGGAA TAGTGGGAGA AAAGGGTGAC
    CGTGGCTCAG TGGGACCCCC AGGCCCTCCT GGAGTCAAGG GGTCGATGGG GCACCCTGGG
    ATACCAGGAG CAGTGGGTTT TCCCGGCATC CCCGGACCAG CAGGTCCGGC AGGAGCCCGC
    GGTGCACCAG GGATCAGAGG GGTGAAAGGC CGCCGGGGTG CCAGAGGGCC TGATGGACCA
    GTAGGCGAGC CGGGGTCACG AGGTGCCAAG GGCCGTCCAG GGGAGCCAGG CAAGCTGGGG
    CCAGATGGGC TGCAGGGGAA GATGGGCGAG ACTGGGGAGA CAGGATCCCG TGGCTTTCCA
    GGTATCCAAG GGCCTTCTGG ACCTCCAGGA ACAAAAGGGG CACCAGGCGA CCCGGGTCCA
    CAAGGACCAC AGGGACCACC TGGACCTTTG GGAGAAATTG GCCATAAGGG ACCGCCTGGC
    CCAATGGGAC AACCTGGCCT CGCAGGTGAA CCTGGCATGA AGGGTGATCC TGGCCAGCTG
    GGAGTTCCTG GAGAGCAAGG CCTCATTGGT CAAAGAGGAG AAATGGGGCT GGAAGGGGAC
    AGTGGGCCAC CTGGTCCAGA CGGAGCCAAG GGAGACAAAG GAGAGCAAGG CCTGGATGGA
    GAGAAAGGAG AAAAAGGCAC TGAAGGACTG AAGGGAAAAG AAGGGCCTCC TGGATATCCC
    GGCATCACAG GTGTCCGAGG ACCAGAAGGG AAACCAGGAA AACAGGGGGA AAAGGGCAGG
    CCTGGCCAGA AGGGCAGCAA GGGCTACCAG GGACAGCTTG GCGAGACAGG ACCTCCAGGG
    AAGGAGGGGC CAAAGGGGAA CCAAGGCCCT AAAGGATCCC GAGGTACCCT GGGACCTATG
    GGTGCTCCAG GAAGGATGGG TGCTCAGGGG GAGCCTGGCT TGAAGGGTTA CCAGGGGCAC
    GCAGGACCAC CAGGGCCAAT TGGACCACCG GGGCCGAAGG GTGAGAAGGG AGAACAAGGT
    GATGACGGGA AGGTGGAAGG CCCACAGGGA CCACCCGGAG ACAGAGGTCC TGTTGGTGAC
    AGAGGAGATC GAGGGGAGCC CGGAGACCCT GGTTATCCTG GCCAAGAAGG GCTTGATGGA
    GAAAGAGGAA AACCTGGACA GCAAGGCTTA CCTGGGGATC CTGGACCACC AGGCCAGCCA
    GGAGCAAAGG GATCCAAAGG TGATGTGGGT CAAAAAGGAA AGCAAGGAGC ACGAGGCAGT
    GCAGGGAGCA GAGGCTCACC GGGCACCCTT GGGCTACCTG GTCCTCGAGG AGTGGTGGGA
    AGGCAGGGCC AAGAGGGTGC TCCTGGAGTG GATGGAGTGC CTGGGAAGGA CGGCGTGCCT
    GGGGTGCTGG GAGAGCAGGG AGATGATGGG GAAGAAGGAG TAACAGGGAT GACAGGCAAA
    AGAGGGAACC CCGGCGTACC GGGACTGCCC GGGGCACAGG GACCACCAGG ATTCAAGGGT
    GAAAGAGGAT TGCCTGGACA GACTGGCCAA ACAGGGAAGC GAGGATCACT GGGAGGAATG
    GGCCTCCCAG GGAGCCCCGG CGATCCAGGA CCCAAAGGCC AGCCGGGAGA CTTTGGGGAG
    GCAGGATTCC CAGGGATCGC GGGCATCTTT GGGCCAAAGG GACCTCCAGG AGACCCCGGT
    TTCAAAGGCA TTCAGGGACC ACGTGGCCCA CCTGGCATCA TGGGAAAAGA AGGAATTATT
    GGTCCACTTG GCATCCTGGG TCCCACAGGA AGCCCGGGGC CAAAGGGGGA CAAAGGCAGC
    CGCGGGGATG TGGGGCTTCG GGGCCCAAGG GGTCCTCCAG GCCCACGAGG ACAGCCTGGC
    CCTCCGGGAC CGCCGGGATT TCCAGCTTCA TTTCAACAAG ATGGCTTTGG GGCTGCTTTC
    CGGACACTGG TTGACTCCAA CGTGGCACTC AAGACAGAGG GTTACCAACA TCCAGACCTT
    CTCATGCTGG ACCATGGAGG GGAGATCTTT AAGACTCTAC ACTACCTCAG CAACCTCATT
    CAGAGCATCA AGAGACCCCT GGGCACCAAG GAAAACCCTG CACGCATCTG CCGAGACCTC
    ATGAACTGTG AACAGAAGAT GAAGGATGGT ACCTACTGGA TCGACCCAAA TCTCGGCTGT
    TCCTCAGACA CCATACAAGT CATCTGCAAC TTCACCAACG GTGGGCAGAC GTGTCTCAGC
    CCCATCACCG TCTCCAAGCT GGATTTTGAC ATCGGCAGAG TCCAGATGAA CTTCCTGCGC
    CTGCTGAGCT CGGAGGTGGT GCAGCACATC ACCATCCACT GCCTGAACAC CTCTGTCTGG
    AGGGAGGGCT CTGCTGAGAA ACCTTCACAG AAAGCCGTGC GCTTCAAGGC GTGGAACGGG
    CAAATGTTTG AGGCAGAGGG GCAGCTCAGG CCGGAGGTGG CAGCTGATGA TTGCAAGATC
    AAGGATGGAC GCTGGCATCG GACTCTCTTT TCCTTTCGGA CGCAGGACAC CCAGCAGCTG
    CCAATCATCA ACATCTACAA CCTCCCCCCA TCCGAGCCAG GAAAGCATTA TCACCTCGAG
    GGTCTCTGCA GCAAGGACAG CTGGGCTGTG TCTTTTGGGT CAACTCTTGC TCTAGCCTTT
    GGCTCAAGGT CTCAAAGAAC CCGTGATCTA CTGAACTCAG CAGGTTGTTG GAAGGAGGAA
    TACGAAGGAC GTAGGGGTAG CGGGTGTCGG TTGGGACGTG ACCAGTGCAA AGGGATCAGG
    GGACGAGGTG TCGCACGCGG CTCCACACAC AAAGCTTTTT CCATGACCAA AGAAGACAAG
    AATGAGAGAA ACCTCAAGCG ACTCGCAGCA TGTGGTTTCC CCACCCTCCC CCCCTATTTT
    TGCCTTACCC GGTTCCCGAG TTACCAAATG TTCTTCCCAT TGAGCAGTAA TTCAGAGATG
    ATAGGGCTTC CCTCCTGGAT GGCCGCGCTG CGCCGGGCTC TCCCAGGCGG AGCTGCAATG
    CGACGCCGGC TGCTCTGCGC ACCGGAACCA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997114.1
    CDS310..5985
    Translation

    Target ORF information:

    RefSeq Version XM_025141346.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025141346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAATGCCA ACGAAGGTGG GGCTGCAAGG AGACGTTGCA CAGCCTCTCG CCTCGCTGCA 
    AAGGGATTCT GCTTTGTCTG GACTGTGTTG TGTTTTAGCT GGTTCCTGGG ATTCACACAG
    GGAAACTCTG AAGATGTGGA TGTTCTTCAG AGGCTGGGCC TGGGAGGGAG AAGGCCATCG
    AGCGGATTGT CCTCATCACA TGCCATTCCT CAAGGGGTCA TTCCTCTCAA ATCAGGGGTT
    ATCTTTACTC AGCGAGCACG CGTCGAAGCA CCCATCAGCA CCATCTTCCC CGCAGGCTCC
    AGCACTGACC TGCTCCTAGT GCTGAGCCTG TGCTCCCACC GCATCAACAA TGCTTTCCTC
    TTTGCAGTCA AGAGCAAAAA GAAAAAACTG CAGCTGGGGG TTCAGTTTGT GCCTGGGAAG
    ATAATAGTGT ACGTGGGCCA CAAGCACTCT GTATATTTTG ATTACAATGT TCACGATGGC
    CAGTGGCACA ATATGGCCAT CAATATCCGT GGCCAGACAG TCACTTTATT TACTTCTTGT
    GGGAAGCACA GAGTACATGC GGATTTGCAT TTTAAAAAGG ACGAGGCATT GGATCCACAT
    GGATCTTTCC TCTTTGGGAA GATGAACCAT CACTCCGTGC AGTTTGAAGG AGCCATCTGC
    CAGTTCGATA TTTATCCTTC CGCCAAGGCA GCTCATCATT ACTGTAAATA CCTGAAAAAA
    CAGTGCAGGC AAGCAGACAC CTACCGGCCA AACCTGCCCC CACTCATCCC CATCCTGCCC
    AGGGATCCCA GTGCCTCCCC GAGTACTCCA CAGCATATGG AGCCCTCGCT TCAGGGTCTG
    AAAAACTTGA CCACTGCCTC CCCATCCTTG GCGCTTGGCA GGGTCACCGC ACCCCTCAAG
    ACTCGGGGTT CAGGTGCTAC AACTGTTGCC TTGGTCTCGT CCCAGCCGCC GCTGCCAAGA
    CAGACAACCA AAGCCACAAA GGTTACAGTG GCCCCATCTC CCGCTCCATC GAGTGCTGGG
    ACGCAGCCGC CCACCCGTTC GCTGCCTCGG GGGACGGTGA CGACCCTCAG GGTGTCTCGG
    CCCACTTCCA GCACACACAC GGAGAAAACT CCCCTGCCTG GTGCCAAAAA GGCCCTGCCA
    GCAAGCCCGA GCCCTGCCAC GGCCAGCAGG AAAGAGACCA AGCAAGAGAC CAAAAAGAAG
    ATCAACCAAA TACCAACCAC CAAGTCCTCT GAAGCACCCA GTACTGTAAA GCCTACCAGG
    AGGAGGGCTG ACAACGCAAC CAGCAGCGTC CACTTTGTCA CCCTGAAGCA AACCCCACAG
    ACCCCAACCA CCGGGCCCCT TCCAAAGAAG CACCTGCCAA CCCCCACCAG GAAAGGGGAT
    CCTGGCAATG TCCCTCTGGT GTACACCAAA CCGCCCCTGG GGGCTGCCCG TCCTGTCTCC
    AGAGCCCAGG CCTTCAACCT CACCACCCCA GCTGCGACCG ATGGCTATCA GATCTTTGAC
    CCCCTTGGGC CCACTCCGTT TCCATTTTTA CTGGGATACC CAGGAGTGAA AGGAGACAGA
    GGACCTCAGG GTCCACCAGG TCTGCCGGGC TTACCAGGAC CACCAGGCAA GAGAGGTTCC
    CGAGGTCCTC CGGGTCCCCA TGGAAACCCT GGTTCACCTG GCCCCCCAGG CCTGAAAGGT
    CAGAAAGGTG ATCCTGGGCT GTCGCCTGGA AAAGCTCGCA GAGGACCAAA GGGAGACGTG
    GGCTTACCTG GTCTGACTGG GTTCCCTGGG CCACCTGGTA GAAAGGGATA CAAAGGCTAT
    CCTGGACCAC CGGGTCACCC TGGAGACCAG GGTGAACGAG GACCAGCCGG CAGCCCTGGT
    GCCAAAGGTT ATCCTGGCAG GCAGGGCTTA CCTGGCCCCA TAGGGGAAGC TGGTCCAAAA
    GGCAGTCGGG GATATATTGG TCTTCCGGGA TTATTTGGGC TACCAGGGGC TGATGGAGAA
    CGAGGGATCC CTGGAGTTCC TGGGAAGAGG GGCAAGATGG GTAGGCCGGG TTTTCCTGGT
    GATTTTGGGG AACGAGGACC TCCAGGCATC GACGGGAACC CAGGAGAATT GGGAGCCCCA
    GGTCCTCCTG GAGTCCTTGG ACTCATTGGT GACATGGGCT CTGTTGGACC TATTGGTTAT
    CCAGGACCAA AGGGCTTAAA GGGGCTGATA GGAAACCCTG GAGAACATGG ACTGAAAGGT
    GATAAGGGTG ATCAGGGAGG AGCAGGTCTT CCAGGAGATA TTGGCTTCCA AGGAGACAAG
    GGTAGCCACG GTTTGCCTGG GGTCCCTGGG GCACGAGGGA AACCAGGCCC TGTGGGAAAA
    GTTGGTGACA TAGGTCCGAT GGGGTTTCCA GGACCACCTG GCCCTGAGGG CTTCCCAGGC
    GACATCGGCC CGCCGGGAGA GAACGGCCCC GAAGGCCCAA AGGGGAAGCC GGGAGCGCGG
    GGTTTGCCTG GGCCCAGAGG GCTGCCTGGA CAAGAGGGAG ATGAAGGACC CTTAGGACCA
    CCAGGAGTCC CTGGTTCAGA GGGTCGACCT GGCAGGAAGG GATTTCCGGG AAGACCTGGT
    CCTGACGGTC CCAAGGGCGA GCCAGGAAAC CACGGCCGGC CGGGGAAAGT TGGTGAGCAG
    GGTCTAATGG GATTCATTGG TCCAGTAGGG GAGCCTGGAA TAGTGGGAGA AAAGGGTGAC
    CGTGGCTCAG TGGGACCCCC AGGCCCTCCT GGAGTCAAGG GGTCGATGGG GCACCCTGGG
    ATACCAGGAG CAGTGGGTTT TCCCGGCATC CCCGGACCAG CAGGTCCGGC AGGAGCCCGC
    GGTGCACCAG GGATCAGAGG GGTGAAAGGC CGCCGGGGTG CCAGAGGGCC TGATGGACCA
    GTAGGCGAGC CGGGGTCACG AGGTGCCAAG GGCCGTCCAG GGGAGCCAGG CAAGCTGGGG
    CCAGATGGGC TGCAGGGGAA GATGGGCGAG ACTGGGGAGA CAGGATCCCG TGGCTTTCCA
    GGTATCCAAG GGCCTTCTGG ACCTCCAGGA ACAAAAGGGG CACCAGGCGA CCCGGGTCCA
    CAAGGACCAC AGGGACCACC TGGACCTTTG GGAGAAATTG GCCATAAGGG ACCGCCTGGC
    CCAATGGGAC AACCTGGCCT CGCAGGTGAA CCTGGCATGA AGGGTGATCC TGGCCAGCTG
    GGAGTTCCTG GAGAGCAAGG CCTCATTGGT CAAAGAGGAG AAATGGGGCT GGAAGGGGAC
    AGTGGGCCAC CTGGTCCAGA CGGAGCCAAG GGAGACAAAG GAGAGCAAGG CCTGGATGGA
    GAGAAAGGAG AAAAAGGCAC TGAAGGACTG AAGGGAAAAG AAGGGCCTCC TGGATATCCC
    GGCATCACAG GTGTCCGAGG ACCAGAAGGG AAACCAGGAA AACAGGGGGA AAAGGGCAGG
    CCTGGCCAGA AGGGCAGCAA GGGCTACCAG GGACAGCTTG GCGAGACAGG ACCTCCAGGG
    AAGGAGGGGC CAAAGGGGAA CCAAGGCCCT AAAGGATCCC GAGGTACCCT GGGACCTATG
    GGTGCTCCAG GAAGGATGGG TGCTCAGGGG GAGCCTGGCT TGAAGGGTTA CCAGGGGCAC
    GCAGGACCAC CAGGGCCAAT TGGACCACCG GGGCCGAAGG GTGAGAAGGG AGAACAAGGT
    GATGACGGGA AGGTGGAAGG CCCACAGGGA CCACCCGGAG ACAGAGGTCC TGTTGGTGAC
    AGAGGAGATC GAGGGGAGCC CGGAGACCCT GGTTATCCTG GCCAAGAAGG GCTTGATGGA
    GAAAGAGGAA AACCTGGACA GCAAGGCTTA CCTGGGGATC CTGGACCACC AGGCCAGCCA
    GGAGCAAAGG GATCCAAAGG TGATGTGGGT CAAAAAGGAA AGCAAGGAGC ACGAGGCAGT
    GCAGGGAGCA GAGGCTCACC GGGCACCCTT GGGCTACCTG GTCCTCGAGG AGTGGTGGGA
    AGGCAGGGCC AAGAGGGTGC TCCTGGAGTG GATGGAGTGC CTGGGAAGGA CGGCGTGCCT
    GGGGTGCTGG GAGAGCAGGG AGATGATGGG GAAGAAGGAG TAACAGGGAT GACAGGCAAA
    AGAGGGAACC CCGGCGTACC GGGACTGCCC GGGGCACAGG GACCACCAGG ATTCAAGGGT
    GAAAGAGGAT TGCCTGGACA GACTGGCCAA ACAGGGAAGC GAGGATCACT GGGAGGAATG
    GGCCTCCCAG GGAGCCCCGG CGATCCAGGA CCCAAAGGCC AGCCGGGAGA CTTTGGGGAG
    GCAGGATTCC CAGGGATCGC GGGCATCTTT GGGCCAAAGG GACCTCCAGG AGACCCCGGT
    TTCAAAGGCA TTCAGGGACC ACGTGGCCCA CCTGGCATCA TGGGAAAAGA AGGAATTATT
    GGTCCACTTG GCATCCTGGG TCCCACAGGA AGCCCGGGGC CAAAGGGGGA CAAAGGCAGC
    CGCGGGGATG TGGGGCTTCG GGGCCCAAGG GGTCCTCCAG GCCCACGAGG ACAGCCTGGC
    CCTCCGGGAC CGCCGGGATT TCCAGCTTCA TTTCAACAAG ATGGCTTTGG GGCTGCTTTC
    CGGACACTGG TTGACTCCAA CGTGGCACTC AAGACAGAGG GTTACCAACA TCCAGACCTT
    CTCATGCTGG ACCATGGAGG GGAGATCTTT AAGACTCTAC ACTACCTCAG CAACCTCATT
    CAGAGCATCA AGAGACCCCT GGGCACCAAG GAAAACCCTG CACGCATCTG CCGAGACCTC
    ATGAACTGTG AACAGAAGAT GAAGGATGGT ACCTACTGGA TCGACCCAAA TCTCGGCTGT
    TCCTCAGACA CCATACAAGT CATCTGCAAC TTCACCAACG GTGGGCAGAC GTGTCTCAGC
    CCCATCACCG TCTCCAAGCT GGATTTTGAC ATCGGCAGAG TCCAGATGAA CTTCCTGCGC
    CTGCTGAGCT CGGAGGTGGT GCAGCACATC ACCATCCACT GCCTGAACAC CTCTGTCTGG
    AGGGAGGGCT CTGCTGAGAA ACCTTCACAG AAAGCCGTGC GCTTCAAGGC GTGGAACGGG
    CAAATGTTTG AGGCAGAGGG GCAGCTCAGG CCGGAGGTGG CAGCTGATGA TTGCAAGATC
    AAGGATGGAC GCTGGCATCG GACTCTCTTT TCCTTTCGGA CGCAGGACAC CCAGCAGCTG
    CCAATCATCA ACATCTACAA CCTCCCCCCA TCCGAGCCAG GAAAGCATTA TCACCTCGAG
    GGTCTCTGCA GCAAGGACAG CTGGGCTGTG TCTTTTGGGT CAACTCTTGC TCTAGCCTTT
    GGCTCAAGGT CTCAAAGAAC CCGTGATCTA CTGAACTCAG CAGGTTGTTG GAAGGAGGAA
    TACGAAGGAC GTAGGGGTAG CGGGTGTCGG TTGGGACGTG ACCAGTGCAA AGGGATCAGG
    GGACGAGGTG TCGCACGCGG CTCCACACAC AAAGCTTTTT CCATGACCAA AGAAGACAAG
    AATGAGAGAA ACCTCAAGCG ACTCGCAGCA TGTGGTTTCC CCACCCTCCC CCCCTATTTT
    TGCCTTACCC GGTTCCCGAG TTACCAAATG TTCTTCCCAT TGAGCAGTAA TTCAGAGATG
    ATAGGGCTTC CCTCCTGGAT GGCCGCGCTG CGCCGGGCTC TCCCAGGCGG AGCTGCAATG
    CGACGCCGGC TGCTCTGCGC ACCGGAACCA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa145170
    Clone ID Related Accession (Same CDS sequence) XM_025141346.1 , XM_025141347.1
    Accession Version XM_025141347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAATGCCA ACGAAGGTGG GGCTGCAAGG AGACGTTGCA CAGCCTCTCG CCTCGCTGCA 
    AAGGGATTCT GCTTTGTCTG GACTGTGTTG TGTTTTAGCT GGTTCCTGGG ATTCACACAG
    GGAAACTCTG AAGATGTGGA TGTTCTTCAG AGGCTGGGCC TGGGAGGGAG AAGGCCATCG
    AGCGGATTGT CCTCATCACA TGCCATTCCT CAAGGGGTCA TTCCTCTCAA ATCAGGGGTT
    ATCTTTACTC AGCGAGCACG CGTCGAAGCA CCCATCAGCA CCATCTTCCC CGCAGGCTCC
    AGCACTGACC TGCTCCTAGT GCTGAGCCTG TGCTCCCACC GCATCAACAA TGCTTTCCTC
    TTTGCAGTCA AGAGCAAAAA GAAAAAACTG CAGCTGGGGG TTCAGTTTGT GCCTGGGAAG
    ATAATAGTGT ACGTGGGCCA CAAGCACTCT GTATATTTTG ATTACAATGT TCACGATGGC
    CAGTGGCACA ATATGGCCAT CAATATCCGT GGCCAGACAG TCACTTTATT TACTTCTTGT
    GGGAAGCACA GAGTACATGC GGATTTGCAT TTTAAAAAGG ACGAGGCATT GGATCCACAT
    GGATCTTTCC TCTTTGGGAA GATGAACCAT CACTCCGTGC AGTTTGAAGG AGCCATCTGC
    CAGTTCGATA TTTATCCTTC CGCCAAGGCA GCTCATCATT ACTGTAAATA CCTGAAAAAA
    CAGTGCAGGC AAGCAGACAC CTACCGGCCA AACCTGCCCC CACTCATCCC CATCCTGCCC
    AGGGATCCCA GTGCCTCCCC GAGTACTCCA CAGCATATGG AGCCCTCGCT TCAGGGTCTG
    AAAAACTTGA CCACTGCCTC CCCATCCTTG GCGCTTGGCA GGGTCACCGC ACCCCTCAAG
    ACTCGGGGTT CAGGTGCTAC AACTGTTGCC TTGGTCTCGT CCCAGCCGCC GCTGCCAAGA
    CAGACAACCA AAGCCACAAA GGTTACAGTG GCCCCATCTC CCGCTCCATC GAGTGCTGGG
    ACGCAGCCGC CCACCCGTTC GCTGCCTCGG GGGACGGTGA CGACCCTCAG GGTGTCTCGG
    CCCACTTCCA GCACACACAC GGAGAAAACT CCCCTGCCTG GTGCCAAAAA GGCCCTGCCA
    GCAAGCCCGA GCCCTGCCAC GGCCAGCAGG AAAGAGACCA AGCAAGAGAC CAAAAAGAAG
    ATCAACCAAA TACCAACCAC CAAGTCCTCT GAAGCACCCA GTACTGTAAA GCCTACCAGG
    AGGAGGGCTG ACAACGCAAC CAGCAGCGTC CACTTTGTCA CCCTGAAGCA AACCCCACAG
    ACCCCAACCA CCGGGCCCCT TCCAAAGAAG CACCTGCCAA CCCCCACCAG GAAAGGGGAT
    CCTGGCAATG TCCCTCTGGT GTACACCAAA CCGCCCCTGG GGGCTGCCCG TCCTGTCTCC
    AGAGCCCAGG CCTTCAACCT CACCACCCCA GCTGCGACCG ATGGCTATCA GATCTTTGAC
    CCCCTTGGGC CCACTCCGTT TCCATTTTTA CTGGGATACC CAGGAGTGAA AGGAGACAGA
    GGACCTCAGG GTCCACCAGG TCTGCCGGGC TTACCAGGAC CACCAGGCAA GAGAGGTTCC
    CGAGGTCCTC CGGGTCCCCA TGGAAACCCT GGTTCACCTG GCCCCCCAGG CCTGAAAGGT
    CAGAAAGGTG ATCCTGGGCT GTCGCCTGGA AAAGCTCGCA GAGGACCAAA GGGAGACGTG
    GGCTTACCTG GTCTGACTGG GTTCCCTGGG CCACCTGGTA GAAAGGGATA CAAAGGCTAT
    CCTGGACCAC CGGGTCACCC TGGAGACCAG GGTGAACGAG GACCAGCCGG CAGCCCTGGT
    GCCAAAGGTT ATCCTGGCAG GCAGGGCTTA CCTGGCCCCA TAGGGGAAGC TGGTCCAAAA
    GGCAGTCGGG GATATATTGG TCTTCCGGGA TTATTTGGGC TACCAGGGGC TGATGGAGAA
    CGAGGGATCC CTGGAGTTCC TGGGAAGAGG GGCAAGATGG GTAGGCCGGG TTTTCCTGGT
    GATTTTGGGG AACGAGGACC TCCAGGCATC GACGGGAACC CAGGAGAATT GGGAGCCCCA
    GGTCCTCCTG GAGTCCTTGG ACTCATTGGT GACATGGGCT CTGTTGGACC TATTGGTTAT
    CCAGGACCAA AGGGCTTAAA GGGGCTGATA GGAAACCCTG GAGAACATGG ACTGAAAGGT
    GATAAGGGTG ATCAGGGAGG AGCAGGTCTT CCAGGAGATA TTGGCTTCCA AGGAGACAAG
    GGTAGCCACG GTTTGCCTGG GGTCCCTGGG GCACGAGGGA AACCAGGCCC TGTGGGAAAA
    GTTGGTGACA TAGGTCCGAT GGGGTTTCCA GGACCACCTG GCCCTGAGGG CTTCCCAGGC
    GACATCGGCC CGCCGGGAGA GAACGGCCCC GAAGGCCCAA AGGGGAAGCC GGGAGCGCGG
    GGTTTGCCTG GGCCCAGAGG GCTGCCTGGA CAAGAGGGAG ATGAAGGACC CTTAGGACCA
    CCAGGAGTCC CTGGTTCAGA GGGTCGACCT GGCAGGAAGG GATTTCCGGG AAGACCTGGT
    CCTGACGGTC CCAAGGGCGA GCCAGGAAAC CACGGCCGGC CGGGGAAAGT TGGTGAGCAG
    GGTCTAATGG GATTCATTGG TCCAGTAGGG GAGCCTGGAA TAGTGGGAGA AAAGGGTGAC
    CGTGGCTCAG TGGGACCCCC AGGCCCTCCT GGAGTCAAGG GGTCGATGGG GCACCCTGGG
    ATACCAGGAG CAGTGGGTTT TCCCGGCATC CCCGGACCAG CAGGTCCGGC AGGAGCCCGC
    GGTGCACCAG GGATCAGAGG GGTGAAAGGC CGCCGGGGTG CCAGAGGGCC TGATGGACCA
    GTAGGCGAGC CGGGGTCACG AGGTGCCAAG GGCCGTCCAG GGGAGCCAGG CAAGCTGGGG
    CCAGATGGGC TGCAGGGGAA GATGGGCGAG ACTGGGGAGA CAGGATCCCG TGGCTTTCCA
    GGTATCCAAG GGCCTTCTGG ACCTCCAGGA ACAAAAGGGG CACCAGGCGA CCCGGGTCCA
    CAAGGACCAC AGGGACCACC TGGACCTTTG GGAGAAATTG GCCATAAGGG ACCGCCTGGC
    CCAATGGGAC AACCTGGCCT CGCAGGTGAA CCTGGCATGA AGGGTGATCC TGGCCAGCTG
    GGAGTTCCTG GAGAGCAAGG CCTCATTGGT CAAAGAGGAG AAATGGGGCT GGAAGGGGAC
    AGTGGGCCAC CTGGTCCAGA CGGAGCCAAG GGAGACAAAG GAGAGCAAGG CCTGGATGGA
    GAGAAAGGAG AAAAAGGCAC TGAAGGACTG AAGGGAAAAG AAGGGCCTCC TGGATATCCC
    GGCATCACAG GTGTCCGAGG ACCAGAAGGG AAACCAGGAA AACAGGGGGA AAAGGGCAGG
    CCTGGCCAGA AGGGCAGCAA GGGCTACCAG GGACAGCTTG GCGAGACAGG ACCTCCAGGG
    AAGGAGGGGC CAAAGGGGAA CCAAGGCCCT AAAGGATCCC GAGGTACCCT GGGACCTATG
    GGTGCTCCAG GAAGGATGGG TGCTCAGGGG GAGCCTGGCT TGAAGGGTTA CCAGGGGCAC
    GCAGGACCAC CAGGGCCAAT TGGACCACCG GGGCCGAAGG GTGAGAAGGG AGAACAAGGT
    GATGACGGGA AGGTGGAAGG CCCACAGGGA CCACCCGGAG ACAGAGGTCC TGTTGGTGAC
    AGAGGAGATC GAGGGGAGCC CGGAGACCCT GGTTATCCTG GCCAAGAAGG GCTTGATGGA
    GAAAGAGGAA AACCTGGACA GCAAGGCTTA CCTGGGGATC CTGGACCACC AGGCCAGCCA
    GGAGCAAAGG GATCCAAAGG TGATGTGGGT CAAAAAGGAA AGCAAGGAGC ACGAGGCAGT
    GCAGGGAGCA GAGGCTCACC GGGCACCCTT GGGCTACCTG GTCCTCGAGG AGTGGTGGGA
    AGGCAGGGCC AAGAGGGTGC TCCTGGAGTG GATGGAGTGC CTGGGAAGGA CGGCGTGCCT
    GGGGTGCTGG GAGAGCAGGG AGATGATGGG GAAGAAGGAG TAACAGGGAT GACAGGCAAA
    AGAGGGAACC CCGGCGTACC GGGACTGCCC GGGGCACAGG GACCACCAGG ATTCAAGGGT
    GAAAGAGGAT TGCCTGGACA GACTGGCCAA ACAGGGAAGC GAGGATCACT GGGAGGAATG
    GGCCTCCCAG GGAGCCCCGG CGATCCAGGA CCCAAAGGCC AGCCGGGAGA CTTTGGGGAG
    GCAGGATTCC CAGGGATCGC GGGCATCTTT GGGCCAAAGG GACCTCCAGG AGACCCCGGT
    TTCAAAGGCA TTCAGGGACC ACGTGGCCCA CCTGGCATCA TGGGAAAAGA AGGAATTATT
    GGTCCACTTG GCATCCTGGG TCCCACAGGA AGCCCGGGGC CAAAGGGGGA CAAAGGCAGC
    CGCGGGGATG TGGGGCTTCG GGGCCCAAGG GGTCCTCCAG GCCCACGAGG ACAGCCTGGC
    CCTCCGGGAC CGCCGGGATT TCCAGCTTCA TTTCAACAAG ATGGCTTTGG GGCTGCTTTC
    CGGACACTGG TTGACTCCAA CGTGGCACTC AAGACAGAGG GTTACCAACA TCCAGACCTT
    CTCATGCTGG ACCATGGAGG GGAGATCTTT AAGACTCTAC ACTACCTCAG CAACCTCATT
    CAGAGCATCA AGAGACCCCT GGGCACCAAG GAAAACCCTG CACGCATCTG CCGAGACCTC
    ATGAACTGTG AACAGAAGAT GAAGGATGGT ACCTACTGGA TCGACCCAAA TCTCGGCTGT
    TCCTCAGACA CCATACAAGT CATCTGCAAC TTCACCAACG GTGGGCAGAC GTGTCTCAGC
    CCCATCACCG TCTCCAAGCT GGATTTTGAC ATCGGCAGAG TCCAGATGAA CTTCCTGCGC
    CTGCTGAGCT CGGAGGTGGT GCAGCACATC ACCATCCACT GCCTGAACAC CTCTGTCTGG
    AGGGAGGGCT CTGCTGAGAA ACCTTCACAG AAAGCCGTGC GCTTCAAGGC GTGGAACGGG
    CAAATGTTTG AGGCAGAGGG GCAGCTCAGG CCGGAGGTGG CAGCTGATGA TTGCAAGATC
    AAGGATGGAC GCTGGCATCG GACTCTCTTT TCCTTTCGGA CGCAGGACAC CCAGCAGCTG
    CCAATCATCA ACATCTACAA CCTCCCCCCA TCCGAGCCAG GAAAGCATTA TCACCTCGAG
    GGTCTCTGCA GCAAGGACAG CTGGGCTGTG TCTTTTGGGT CAACTCTTGC TCTAGCCTTT
    GGCTCAAGGT CTCAAAGAAC CCGTGATCTA CTGAACTCAG CAGGTTGTTG GAAGGAGGAA
    TACGAAGGAC GTAGGGGTAG CGGGTGTCGG TTGGGACGTG ACCAGTGCAA AGGGATCAGG
    GGACGAGGTG TCGCACGCGG CTCCACACAC AAAGCTTTTT CCATGACCAA AGAAGACAAG
    AATGAGAGAA ACCTCAAGCG ACTCGCAGCA TGTGGTTTCC CCACCCTCCC CCCCTATTTT
    TGCCTTACCC GGTTCCCGAG TTACCAAATG TTCTTCCCAT TGAGCAGTAA TTCAGAGATG
    ATAGGGCTTC CCTCCTGGAT GGCCGCGCTG CGCCGGGCTC TCCCAGGCGG AGCTGCAATG
    CGACGCCGGC TGCTCTGCGC ACCGGAACCA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997115.1
    CDS182..5857
    Translation

    Target ORF information:

    RefSeq Version XM_025141347.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025141347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAATGCCA ACGAAGGTGG GGCTGCAAGG AGACGTTGCA CAGCCTCTCG CCTCGCTGCA 
    AAGGGATTCT GCTTTGTCTG GACTGTGTTG TGTTTTAGCT GGTTCCTGGG ATTCACACAG
    GGAAACTCTG AAGATGTGGA TGTTCTTCAG AGGCTGGGCC TGGGAGGGAG AAGGCCATCG
    AGCGGATTGT CCTCATCACA TGCCATTCCT CAAGGGGTCA TTCCTCTCAA ATCAGGGGTT
    ATCTTTACTC AGCGAGCACG CGTCGAAGCA CCCATCAGCA CCATCTTCCC CGCAGGCTCC
    AGCACTGACC TGCTCCTAGT GCTGAGCCTG TGCTCCCACC GCATCAACAA TGCTTTCCTC
    TTTGCAGTCA AGAGCAAAAA GAAAAAACTG CAGCTGGGGG TTCAGTTTGT GCCTGGGAAG
    ATAATAGTGT ACGTGGGCCA CAAGCACTCT GTATATTTTG ATTACAATGT TCACGATGGC
    CAGTGGCACA ATATGGCCAT CAATATCCGT GGCCAGACAG TCACTTTATT TACTTCTTGT
    GGGAAGCACA GAGTACATGC GGATTTGCAT TTTAAAAAGG ACGAGGCATT GGATCCACAT
    GGATCTTTCC TCTTTGGGAA GATGAACCAT CACTCCGTGC AGTTTGAAGG AGCCATCTGC
    CAGTTCGATA TTTATCCTTC CGCCAAGGCA GCTCATCATT ACTGTAAATA CCTGAAAAAA
    CAGTGCAGGC AAGCAGACAC CTACCGGCCA AACCTGCCCC CACTCATCCC CATCCTGCCC
    AGGGATCCCA GTGCCTCCCC GAGTACTCCA CAGCATATGG AGCCCTCGCT TCAGGGTCTG
    AAAAACTTGA CCACTGCCTC CCCATCCTTG GCGCTTGGCA GGGTCACCGC ACCCCTCAAG
    ACTCGGGGTT CAGGTGCTAC AACTGTTGCC TTGGTCTCGT CCCAGCCGCC GCTGCCAAGA
    CAGACAACCA AAGCCACAAA GGTTACAGTG GCCCCATCTC CCGCTCCATC GAGTGCTGGG
    ACGCAGCCGC CCACCCGTTC GCTGCCTCGG GGGACGGTGA CGACCCTCAG GGTGTCTCGG
    CCCACTTCCA GCACACACAC GGAGAAAACT CCCCTGCCTG GTGCCAAAAA GGCCCTGCCA
    GCAAGCCCGA GCCCTGCCAC GGCCAGCAGG AAAGAGACCA AGCAAGAGAC CAAAAAGAAG
    ATCAACCAAA TACCAACCAC CAAGTCCTCT GAAGCACCCA GTACTGTAAA GCCTACCAGG
    AGGAGGGCTG ACAACGCAAC CAGCAGCGTC CACTTTGTCA CCCTGAAGCA AACCCCACAG
    ACCCCAACCA CCGGGCCCCT TCCAAAGAAG CACCTGCCAA CCCCCACCAG GAAAGGGGAT
    CCTGGCAATG TCCCTCTGGT GTACACCAAA CCGCCCCTGG GGGCTGCCCG TCCTGTCTCC
    AGAGCCCAGG CCTTCAACCT CACCACCCCA GCTGCGACCG ATGGCTATCA GATCTTTGAC
    CCCCTTGGGC CCACTCCGTT TCCATTTTTA CTGGGATACC CAGGAGTGAA AGGAGACAGA
    GGACCTCAGG GTCCACCAGG TCTGCCGGGC TTACCAGGAC CACCAGGCAA GAGAGGTTCC
    CGAGGTCCTC CGGGTCCCCA TGGAAACCCT GGTTCACCTG GCCCCCCAGG CCTGAAAGGT
    CAGAAAGGTG ATCCTGGGCT GTCGCCTGGA AAAGCTCGCA GAGGACCAAA GGGAGACGTG
    GGCTTACCTG GTCTGACTGG GTTCCCTGGG CCACCTGGTA GAAAGGGATA CAAAGGCTAT
    CCTGGACCAC CGGGTCACCC TGGAGACCAG GGTGAACGAG GACCAGCCGG CAGCCCTGGT
    GCCAAAGGTT ATCCTGGCAG GCAGGGCTTA CCTGGCCCCA TAGGGGAAGC TGGTCCAAAA
    GGCAGTCGGG GATATATTGG TCTTCCGGGA TTATTTGGGC TACCAGGGGC TGATGGAGAA
    CGAGGGATCC CTGGAGTTCC TGGGAAGAGG GGCAAGATGG GTAGGCCGGG TTTTCCTGGT
    GATTTTGGGG AACGAGGACC TCCAGGCATC GACGGGAACC CAGGAGAATT GGGAGCCCCA
    GGTCCTCCTG GAGTCCTTGG ACTCATTGGT GACATGGGCT CTGTTGGACC TATTGGTTAT
    CCAGGACCAA AGGGCTTAAA GGGGCTGATA GGAAACCCTG GAGAACATGG ACTGAAAGGT
    GATAAGGGTG ATCAGGGAGG AGCAGGTCTT CCAGGAGATA TTGGCTTCCA AGGAGACAAG
    GGTAGCCACG GTTTGCCTGG GGTCCCTGGG GCACGAGGGA AACCAGGCCC TGTGGGAAAA
    GTTGGTGACA TAGGTCCGAT GGGGTTTCCA GGACCACCTG GCCCTGAGGG CTTCCCAGGC
    GACATCGGCC CGCCGGGAGA GAACGGCCCC GAAGGCCCAA AGGGGAAGCC GGGAGCGCGG
    GGTTTGCCTG GGCCCAGAGG GCTGCCTGGA CAAGAGGGAG ATGAAGGACC CTTAGGACCA
    CCAGGAGTCC CTGGTTCAGA GGGTCGACCT GGCAGGAAGG GATTTCCGGG AAGACCTGGT
    CCTGACGGTC CCAAGGGCGA GCCAGGAAAC CACGGCCGGC CGGGGAAAGT TGGTGAGCAG
    GGTCTAATGG GATTCATTGG TCCAGTAGGG GAGCCTGGAA TAGTGGGAGA AAAGGGTGAC
    CGTGGCTCAG TGGGACCCCC AGGCCCTCCT GGAGTCAAGG GGTCGATGGG GCACCCTGGG
    ATACCAGGAG CAGTGGGTTT TCCCGGCATC CCCGGACCAG CAGGTCCGGC AGGAGCCCGC
    GGTGCACCAG GGATCAGAGG GGTGAAAGGC CGCCGGGGTG CCAGAGGGCC TGATGGACCA
    GTAGGCGAGC CGGGGTCACG AGGTGCCAAG GGCCGTCCAG GGGAGCCAGG CAAGCTGGGG
    CCAGATGGGC TGCAGGGGAA GATGGGCGAG ACTGGGGAGA CAGGATCCCG TGGCTTTCCA
    GGTATCCAAG GGCCTTCTGG ACCTCCAGGA ACAAAAGGGG CACCAGGCGA CCCGGGTCCA
    CAAGGACCAC AGGGACCACC TGGACCTTTG GGAGAAATTG GCCATAAGGG ACCGCCTGGC
    CCAATGGGAC AACCTGGCCT CGCAGGTGAA CCTGGCATGA AGGGTGATCC TGGCCAGCTG
    GGAGTTCCTG GAGAGCAAGG CCTCATTGGT CAAAGAGGAG AAATGGGGCT GGAAGGGGAC
    AGTGGGCCAC CTGGTCCAGA CGGAGCCAAG GGAGACAAAG GAGAGCAAGG CCTGGATGGA
    GAGAAAGGAG AAAAAGGCAC TGAAGGACTG AAGGGAAAAG AAGGGCCTCC TGGATATCCC
    GGCATCACAG GTGTCCGAGG ACCAGAAGGG AAACCAGGAA AACAGGGGGA AAAGGGCAGG
    CCTGGCCAGA AGGGCAGCAA GGGCTACCAG GGACAGCTTG GCGAGACAGG ACCTCCAGGG
    AAGGAGGGGC CAAAGGGGAA CCAAGGCCCT AAAGGATCCC GAGGTACCCT GGGACCTATG
    GGTGCTCCAG GAAGGATGGG TGCTCAGGGG GAGCCTGGCT TGAAGGGTTA CCAGGGGCAC
    GCAGGACCAC CAGGGCCAAT TGGACCACCG GGGCCGAAGG GTGAGAAGGG AGAACAAGGT
    GATGACGGGA AGGTGGAAGG CCCACAGGGA CCACCCGGAG ACAGAGGTCC TGTTGGTGAC
    AGAGGAGATC GAGGGGAGCC CGGAGACCCT GGTTATCCTG GCCAAGAAGG GCTTGATGGA
    GAAAGAGGAA AACCTGGACA GCAAGGCTTA CCTGGGGATC CTGGACCACC AGGCCAGCCA
    GGAGCAAAGG GATCCAAAGG TGATGTGGGT CAAAAAGGAA AGCAAGGAGC ACGAGGCAGT
    GCAGGGAGCA GAGGCTCACC GGGCACCCTT GGGCTACCTG GTCCTCGAGG AGTGGTGGGA
    AGGCAGGGCC AAGAGGGTGC TCCTGGAGTG GATGGAGTGC CTGGGAAGGA CGGCGTGCCT
    GGGGTGCTGG GAGAGCAGGG AGATGATGGG GAAGAAGGAG TAACAGGGAT GACAGGCAAA
    AGAGGGAACC CCGGCGTACC GGGACTGCCC GGGGCACAGG GACCACCAGG ATTCAAGGGT
    GAAAGAGGAT TGCCTGGACA GACTGGCCAA ACAGGGAAGC GAGGATCACT GGGAGGAATG
    GGCCTCCCAG GGAGCCCCGG CGATCCAGGA CCCAAAGGCC AGCCGGGAGA CTTTGGGGAG
    GCAGGATTCC CAGGGATCGC GGGCATCTTT GGGCCAAAGG GACCTCCAGG AGACCCCGGT
    TTCAAAGGCA TTCAGGGACC ACGTGGCCCA CCTGGCATCA TGGGAAAAGA AGGAATTATT
    GGTCCACTTG GCATCCTGGG TCCCACAGGA AGCCCGGGGC CAAAGGGGGA CAAAGGCAGC
    CGCGGGGATG TGGGGCTTCG GGGCCCAAGG GGTCCTCCAG GCCCACGAGG ACAGCCTGGC
    CCTCCGGGAC CGCCGGGATT TCCAGCTTCA TTTCAACAAG ATGGCTTTGG GGCTGCTTTC
    CGGACACTGG TTGACTCCAA CGTGGCACTC AAGACAGAGG GTTACCAACA TCCAGACCTT
    CTCATGCTGG ACCATGGAGG GGAGATCTTT AAGACTCTAC ACTACCTCAG CAACCTCATT
    CAGAGCATCA AGAGACCCCT GGGCACCAAG GAAAACCCTG CACGCATCTG CCGAGACCTC
    ATGAACTGTG AACAGAAGAT GAAGGATGGT ACCTACTGGA TCGACCCAAA TCTCGGCTGT
    TCCTCAGACA CCATACAAGT CATCTGCAAC TTCACCAACG GTGGGCAGAC GTGTCTCAGC
    CCCATCACCG TCTCCAAGCT GGATTTTGAC ATCGGCAGAG TCCAGATGAA CTTCCTGCGC
    CTGCTGAGCT CGGAGGTGGT GCAGCACATC ACCATCCACT GCCTGAACAC CTCTGTCTGG
    AGGGAGGGCT CTGCTGAGAA ACCTTCACAG AAAGCCGTGC GCTTCAAGGC GTGGAACGGG
    CAAATGTTTG AGGCAGAGGG GCAGCTCAGG CCGGAGGTGG CAGCTGATGA TTGCAAGATC
    AAGGATGGAC GCTGGCATCG GACTCTCTTT TCCTTTCGGA CGCAGGACAC CCAGCAGCTG
    CCAATCATCA ACATCTACAA CCTCCCCCCA TCCGAGCCAG GAAAGCATTA TCACCTCGAG
    GGTCTCTGCA GCAAGGACAG CTGGGCTGTG TCTTTTGGGT CAACTCTTGC TCTAGCCTTT
    GGCTCAAGGT CTCAAAGAAC CCGTGATCTA CTGAACTCAG CAGGTTGTTG GAAGGAGGAA
    TACGAAGGAC GTAGGGGTAG CGGGTGTCGG TTGGGACGTG ACCAGTGCAA AGGGATCAGG
    GGACGAGGTG TCGCACGCGG CTCCACACAC AAAGCTTTTT CCATGACCAA AGAAGACAAG
    AATGAGAGAA ACCTCAAGCG ACTCGCAGCA TGTGGTTTCC CCACCCTCCC CCCCTATTTT
    TGCCTTACCC GGTTCCCGAG TTACCAAATG TTCTTCCCAT TGAGCAGTAA TTCAGAGATG
    ATAGGGCTTC CCTCCTGGAT GGCCGCGCTG CGCCGGGCTC TCCCAGGCGG AGCTGCAATG
    CGACGCCGGC TGCTCTGCGC ACCGGAACCA TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa145171
    Clone ID Related Accession (Same CDS sequence) XM_025141348.1
    Accession Version XM_025141348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAATATG GCTACAGGAA GACAAGAAGT AGACGTTGTT GTAAGCTGCT GGGACAAATG 
    AATGCCAACG AAGGTGGGGC TGCAAGGAGA CGTTGCACAG CCTCTCGCCT CGCTGCAAAG
    GGATTCTGCT TTGTCTGGAC TGTGTTGTGT TTTAGCTGGT TCCTGGGATT CACACAGGGA
    AACTCTGAAG ATGTGGATGT TCTTCAGAGG CTGGGCCTGG GAGGGAGAAG GCCATCGAGC
    GGATTGTCCT CATCACATGC CATTCCTCAA GGGGTCATTC CTCTCAAATC AGGGGTTATC
    TTTACTCAGC GAGCACGCGT CGAAGCACCC ATCAGCACCA TCTTCCCCGC AGGCTCCAGC
    ACTGACCTGC TCCTAGTGCT GAGCCTGTGC TCCCACCGCA TCAACAATGC TTTCCTCTTT
    GCAGTCAAGA GCAAAAAGAA AAAACTGCAG CTGGGGGTTC AGTTTGTGCC TGGGAAGATA
    ATAGTGTACG TGGGCCACAA GCACTCTGTA TATTTTGATT ACAATGTTCA CGATGGCCAG
    TGGCACAATA TGGCCATCAA TATCCGTGGC CAGACAGTCA CTTTATTTAC TTCTTGTGGG
    AAGCACAGAG TACATGCGGA TTTGCATTTT AAAAAGGACG AGGCATTGGA TCCACATGGA
    TCTTTCCTCT TTGGGAAGAT GAACCATCAC TCCGTGCAGT TTGAAGGAGC CATCTGCCAG
    TTCGATATTT ATCCTTCCGC CAAGGCAGCT CATCATTACT GTAAATACCT GAAAAAACAG
    TGCAGGCAAG CAGACACCTA CCGGCCAAAC CTGCCCCCAC TCATCCCCAT CCTGCCCAGG
    GATCCCAGTG CCTCCCCGAG TACTCCACAG CATATGGAGC CCTCGCTTCA GGGTCTGAAA
    AACTTGACCA CTGCCTCCCC ATCCTTGGCG CTTGGCAGGG TCACCGCACC CCTCAAGACT
    CGGGGTTCAG GTGCTACAAC TGTTGCCTTG GTCTCGTCCC AGCCGCCGCT GCCAAGACAG
    ACAACCAAAG CCACAAAGGT TACAGTGGCC CCATCTCCCG CTCCATCGAG TGCTGGGACG
    CAGCCGCCCA CCCGTTCGCT GCCTCGGGGG ACGGTGACGA CCCTCAGGGT GTCTCGGCCC
    ACTTCCAGCA CACACACGGA GAAAACTCCC CTGCCTGGTG CCAAAAAGGC CCTGCCAGCA
    AGCCCGAGCC CTGCCACGGC CAGCAGGAAA GAGACCAAGC AAGAGACCAA AAAGAAGATC
    AACCAAATAC CAACCACCAA GTCCTCTGAA GCACCCAGTA CTGTAAAGCC TACCAGGAGG
    AGGGCTGACA ACGCAACCAG CAGCGTCCAC TTTGTCACCC TGAAGCAAAC CCCACAGACC
    CCAACCACCG GGCCCCTTCC AAAGAAGCAC CTGCCAACCC CCACCAGGAA AGGGGATCCT
    GGCAATGTCC CTCTGGTGTA CACCAAACCG CCCCTGGGGG CTGCCCGTCC TGTCTCCAGA
    GCCCAGGCCT TCAACCTCAC CACCCCAGCT GCGACCGATG GCTATCAGAT CTTTGACCCC
    CTTGGGCCCA CTCCGTTTCC ATTTTTACTG GGATACCCAG GAGTGAAAGG AGACAGAGGA
    CCTCAGGGTC CACCAGGTCT GCCGGGCTTA CCAGGACCAC CAGGCAAGAG AGGTTCCCGA
    GGTCCTCCGG GTCCCCATGG AAACCCTGGT TCACCTGGCC CCCCAGGCCT GAAAGGTCAG
    AAAGGTGATC CTGGGCTGTC GCCTGGAAAA GCTCGCAGAG GACCAAAGGG AGACGTGGGC
    TTACCTGGTC TGACTGGGTT CCCTGGGCCA CCTGGTAGAA AGGGATACAA AGGCTATCCT
    GGACCACCGG GTCACCCTGG AGACCAGGGT GAACGAGGAC CAGCCGGCAG CCCTGGTGCC
    AAAGGTTATC CTGGCAGGCA GGGCTTACCT GGCCCCATAG GGGAAGCTGG TCCAAAAGGC
    AGTCGGGGAT ATATTGGTCT TCCGGGATTA TTTGGGCTAC CAGGGGCTGA TGGAGAACGA
    GGGATCCCTG GAGTTCCTGG GAAGAGGGGC AAGATGGGTA GGCCGGGTTT TCCTGGTGAT
    TTTGGGGAAC GAGGACCTCC AGGCATCGAC GGGAACCCAG GAGAATTGGG AGCCCCAGGT
    CCTCCTGGAG TCCTTGGACT CATTGGTGAC ATGGGCTCTG TTGGACCTAT TGGTTATCCA
    GGACCAAAGG GCTTAAAGGG GCTGATAGGA AACCCTGGAG AACATGGACT GAAAGGTGAT
    AAGGGTGATC AGGGAGGAGC AGGTCTTCCA GGAGATATTG GCTTCCAAGG AGACAAGGGT
    AGCCACGGTT TGCCTGGGGT CCCTGGGGCA CGAGGGAAAC CAGGCCCTGT GGGAAAAGTT
    GGTGACATAG GTCCGATGGG GTTTCCAGGA CCACCTGGCC CTGAGGGCTT CCCAGGCGAC
    ATCGGCCCGC CGGGAGAGAA CGGCCCCGAA GGCCCAAAGG GGAAGCCGGG AGCGCGGGGT
    TTGCCTGGGC CCAGAGGGCT GCCTGGACAA GAGGGAGATG AAGGACCCTT AGGACCACCA
    GGAGTCCCTG GTTCAGAGGG TCGACCTGGC AGGAAGGGAT TTCCGGGAAG ACCTGGTCCT
    GACGGTCCCA AGGGCGAGCC AGGAAACCAC GGCCGGCCGG GGAAAGTTGG TGAGCAGGGT
    CTAATGGGAT TCATTGGTCC AGTAGGGGAG CCTGGAATAG TGGGAGAAAA GGGTGACCGT
    GGCTCAGTGG GACCCCCAGG CCCTCCTGGA GTCAAGGGGT CGATGGGGCA CCCTGGGATA
    CCAGGAGCAG TGGGTTTTCC CGGCATCCCC GGACCAGCAG GTCCGGCAGG AGCCCGCGGT
    GCACCAGGGA TCAGAGGGGT GAAAGGCCGC CGGGGTGCCA GAGGGCCTGA TGGACCAGTA
    GGCGAGCCGG GGTCACGAGG TGCCAAGGGC CGTCCAGGGG AGCCAGGCAA GCTGGGGCCA
    GATGGGCTGC AGGGGAAGAT GGGCGAGACT GGGGAGACAG GATCCCGTGG CTTTCCAGGT
    ATCCAAGGGC CTTCTGGACC TCCAGGAACA AAAGGGGCAC CAGGCGACCC GGGTCCACAA
    GGACCACAGG GACCACCTGG ACCTTTGGGA GAAATTGGCC ATAAGGGACC GCCTGGCCCA
    ATGGGACAAC CTGGCCTCGC AGGTGAACCT GGCATGAAGG GTGATCCTGG CCAGCTGGGA
    GTTCCTGGAG AGCAAGGCCT CATTGGTCAA AGAGGAGAAA TGGGGCTGGA AGGGGACAGT
    GGGCCACCTG GTCCAGACGG AGCCAAGGGA GACAAAGGAG AGCAAGGCCT GGATGGAGAG
    AAAGGAGAAA AAGGCACTGA AGGACTGAAG GGAAAAGAAG GGCCTCCTGG ATATCCCGGC
    ATCACAGGTG TCCGAGGACC AGAAGGGAAA CCAGGAAAAC AGGGGGAAAA GGGCAGGCCT
    GGCCAGAAGG GCAGCAAGGG CTACCAGGGA CAGCTTGGCG AGACAGGACC TCCAGGGAAG
    GAGGGGCCAA AGGGGAACCA AGGCCCTAAA GGATCCCGAG GTACCCTGGG ACCTATGGGT
    GCTCCAGGAA GGATGGGTGC TCAGGGGGAG CCTGGCTTGA AGGGTTACCA GGGGCACGCA
    GGACCACCAG GGCCAATTGG ACCACCGGGG CCGAAGGGTG AGAAGGGAGA ACAAGGTGAT
    GACGGGAAGG TGGAAGGCCC ACAGGGACCA CCCGGAGACA GAGGTCCTGT TGGTGACAGA
    GGAGATCGAG GGGAGCCCGG AGACCCTGGT TATCCTGGCC AAGAAGGGCT TGATGGAGAA
    AGAGGAAAAC CTGGACAGCA AGGCTTACCT GGGGATCCTG GACCACCAGG CCAGCCAGGA
    GCAAAGGGAT CCAAAGGTGA TGTGGGTCAA AAAGGAAAGC AAGGAGCACG AGGCAGTGCA
    GGGAGCAGAG GCTCACCGGG CACCCTTGGG CTACCTGGTC CTCGAGGAGT GGTGGGAAGG
    CAGGGCCAAG AGGGTGCTCC TGGAGTGGAT GGAGTGCCTG GGAAGGACGG CGTGCCTGGG
    GTGCTGGGAG AGCAGGGAGA TGATGGGGAA GAAGGAGTAA CAGGGATGAC AGGCAAAAGA
    GGGAACCCCG GCGTACCGGG ACTGCCCGGG GCACAGGGAC CACCAGGATT CAAGGGTGAA
    AGAGGATTGC CTGGACAGAC TGGCCAAACA GGGAAGCGAG GATCACTGGG AGGAATGGGC
    CTCCCAGGGA GCCCCGGCGA TCCAGGACCC AAAGGCCAGC CGGGAGACTT TGGGGAGGCA
    GGATTCCCAG GGATCGCGGG CATCTTTGGG CCAAAGGGAC CTCCAGGAGA CCCCGGTTTC
    AAAGGCATTC AGGGACCACG TGGCCCACCT GGCATCATGG GAAAAGAAGG AATTATTGGT
    CCACTTGGCA TCCTGGGTCC CACAGGAAGC CCGGGGCCAA AGGGGGACAA AGGCAGCCGC
    GGGGATGTGG GGCTTCGGGG CCCAAGGGGT CCTCCAGGCC CACGAGGACA GCCTGGCCCT
    CCGGGACCGC CGGGATTTCC AGCTTCATTT CAACAAGATG GCTTTGGGGC TGCTTTCCGG
    ACACTGGTTG ACTCCAACGT GGCACTCAAG ACAGAGGGTT ACCAACATCC AGACCTTCTC
    ATGCTGGACC ATGGAGGGGA GATCTTTAAG ACTCTACACT ACCTCAGCAA CCTCATTCAG
    AGCATCAAGA GACCCCTGGG CACCAAGGAA AACCCTGCAC GCATCTGCCG AGACCTCATG
    AACTGTGAAC AGAAGATGAA GGATGGTACC TACTGGATCG ACCCAAATCT CGGCTGTTCC
    TCAGACACCA TACAAGTCAT CTGCAACTTC ACCAACGGTG GGCAGACGTG TCTCAGCCCC
    ATCACCGTCT CCAAGCTGGA TTTTGACATC GGCAGAGTCC AGATGAACTT CCTGCGCCTG
    CTGAGCTCGG AGGTGGTGCA GCACATCACC ATCCACTGCC TGAACACCTC TGTCTGGAGG
    GAGGGCTCTG CTGAGAAACC TTCACAGAAA GCCGTGCGCT TCAAGGCGTG GAACGGGCAA
    ATGTTTGAGG CAGAGGGGCA GCTCAGGCCG GAGGTGGCAG CTGATGATTG CAAGATCAAG
    GATGGACGCT GGCATCGGAC TCTCTTTTCC TTTCGGACGC AGGACACCCA GCAGCTGCCA
    ATCATCAACA TCTACAACCT CCCCCCATCC GAGCCAGGAA AGCATTATCA CCTCGAGGTC
    GGCCCCGTGT GCTTCCTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024997116.1
    CDS16..5316
    Translation

    Target ORF information:

    RefSeq Version XM_025141348.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025141348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAATATG GCTACAGGAA GACAAGAAGT AGACGTTGTT GTAAGCTGCT GGGACAAATG 
    AATGCCAACG AAGGTGGGGC TGCAAGGAGA CGTTGCACAG CCTCTCGCCT CGCTGCAAAG
    GGATTCTGCT TTGTCTGGAC TGTGTTGTGT TTTAGCTGGT TCCTGGGATT CACACAGGGA
    AACTCTGAAG ATGTGGATGT TCTTCAGAGG CTGGGCCTGG GAGGGAGAAG GCCATCGAGC
    GGATTGTCCT CATCACATGC CATTCCTCAA GGGGTCATTC CTCTCAAATC AGGGGTTATC
    TTTACTCAGC GAGCACGCGT CGAAGCACCC ATCAGCACCA TCTTCCCCGC AGGCTCCAGC
    ACTGACCTGC TCCTAGTGCT GAGCCTGTGC TCCCACCGCA TCAACAATGC TTTCCTCTTT
    GCAGTCAAGA GCAAAAAGAA AAAACTGCAG CTGGGGGTTC AGTTTGTGCC TGGGAAGATA
    ATAGTGTACG TGGGCCACAA GCACTCTGTA TATTTTGATT ACAATGTTCA CGATGGCCAG
    TGGCACAATA TGGCCATCAA TATCCGTGGC CAGACAGTCA CTTTATTTAC TTCTTGTGGG
    AAGCACAGAG TACATGCGGA TTTGCATTTT AAAAAGGACG AGGCATTGGA TCCACATGGA
    TCTTTCCTCT TTGGGAAGAT GAACCATCAC TCCGTGCAGT TTGAAGGAGC CATCTGCCAG
    TTCGATATTT ATCCTTCCGC CAAGGCAGCT CATCATTACT GTAAATACCT GAAAAAACAG
    TGCAGGCAAG CAGACACCTA CCGGCCAAAC CTGCCCCCAC TCATCCCCAT CCTGCCCAGG
    GATCCCAGTG CCTCCCCGAG TACTCCACAG CATATGGAGC CCTCGCTTCA GGGTCTGAAA
    AACTTGACCA CTGCCTCCCC ATCCTTGGCG CTTGGCAGGG TCACCGCACC CCTCAAGACT
    CGGGGTTCAG GTGCTACAAC TGTTGCCTTG GTCTCGTCCC AGCCGCCGCT GCCAAGACAG
    ACAACCAAAG CCACAAAGGT TACAGTGGCC CCATCTCCCG CTCCATCGAG TGCTGGGACG
    CAGCCGCCCA CCCGTTCGCT GCCTCGGGGG ACGGTGACGA CCCTCAGGGT GTCTCGGCCC
    ACTTCCAGCA CACACACGGA GAAAACTCCC CTGCCTGGTG CCAAAAAGGC CCTGCCAGCA
    AGCCCGAGCC CTGCCACGGC CAGCAGGAAA GAGACCAAGC AAGAGACCAA AAAGAAGATC
    AACCAAATAC CAACCACCAA GTCCTCTGAA GCACCCAGTA CTGTAAAGCC TACCAGGAGG
    AGGGCTGACA ACGCAACCAG CAGCGTCCAC TTTGTCACCC TGAAGCAAAC CCCACAGACC
    CCAACCACCG GGCCCCTTCC AAAGAAGCAC CTGCCAACCC CCACCAGGAA AGGGGATCCT
    GGCAATGTCC CTCTGGTGTA CACCAAACCG CCCCTGGGGG CTGCCCGTCC TGTCTCCAGA
    GCCCAGGCCT TCAACCTCAC CACCCCAGCT GCGACCGATG GCTATCAGAT CTTTGACCCC
    CTTGGGCCCA CTCCGTTTCC ATTTTTACTG GGATACCCAG GAGTGAAAGG AGACAGAGGA
    CCTCAGGGTC CACCAGGTCT GCCGGGCTTA CCAGGACCAC CAGGCAAGAG AGGTTCCCGA
    GGTCCTCCGG GTCCCCATGG AAACCCTGGT TCACCTGGCC CCCCAGGCCT GAAAGGTCAG
    AAAGGTGATC CTGGGCTGTC GCCTGGAAAA GCTCGCAGAG GACCAAAGGG AGACGTGGGC
    TTACCTGGTC TGACTGGGTT CCCTGGGCCA CCTGGTAGAA AGGGATACAA AGGCTATCCT
    GGACCACCGG GTCACCCTGG AGACCAGGGT GAACGAGGAC CAGCCGGCAG CCCTGGTGCC
    AAAGGTTATC CTGGCAGGCA GGGCTTACCT GGCCCCATAG GGGAAGCTGG TCCAAAAGGC
    AGTCGGGGAT ATATTGGTCT TCCGGGATTA TTTGGGCTAC CAGGGGCTGA TGGAGAACGA
    GGGATCCCTG GAGTTCCTGG GAAGAGGGGC AAGATGGGTA GGCCGGGTTT TCCTGGTGAT
    TTTGGGGAAC GAGGACCTCC AGGCATCGAC GGGAACCCAG GAGAATTGGG AGCCCCAGGT
    CCTCCTGGAG TCCTTGGACT CATTGGTGAC ATGGGCTCTG TTGGACCTAT TGGTTATCCA
    GGACCAAAGG GCTTAAAGGG GCTGATAGGA AACCCTGGAG AACATGGACT GAAAGGTGAT
    AAGGGTGATC AGGGAGGAGC AGGTCTTCCA GGAGATATTG GCTTCCAAGG AGACAAGGGT
    AGCCACGGTT TGCCTGGGGT CCCTGGGGCA CGAGGGAAAC CAGGCCCTGT GGGAAAAGTT
    GGTGACATAG GTCCGATGGG GTTTCCAGGA CCACCTGGCC CTGAGGGCTT CCCAGGCGAC
    ATCGGCCCGC CGGGAGAGAA CGGCCCCGAA GGCCCAAAGG GGAAGCCGGG AGCGCGGGGT
    TTGCCTGGGC CCAGAGGGCT GCCTGGACAA GAGGGAGATG AAGGACCCTT AGGACCACCA
    GGAGTCCCTG GTTCAGAGGG TCGACCTGGC AGGAAGGGAT TTCCGGGAAG ACCTGGTCCT
    GACGGTCCCA AGGGCGAGCC AGGAAACCAC GGCCGGCCGG GGAAAGTTGG TGAGCAGGGT
    CTAATGGGAT TCATTGGTCC AGTAGGGGAG CCTGGAATAG TGGGAGAAAA GGGTGACCGT
    GGCTCAGTGG GACCCCCAGG CCCTCCTGGA GTCAAGGGGT CGATGGGGCA CCCTGGGATA
    CCAGGAGCAG TGGGTTTTCC CGGCATCCCC GGACCAGCAG GTCCGGCAGG AGCCCGCGGT
    GCACCAGGGA TCAGAGGGGT GAAAGGCCGC CGGGGTGCCA GAGGGCCTGA TGGACCAGTA
    GGCGAGCCGG GGTCACGAGG TGCCAAGGGC CGTCCAGGGG AGCCAGGCAA GCTGGGGCCA
    GATGGGCTGC AGGGGAAGAT GGGCGAGACT GGGGAGACAG GATCCCGTGG CTTTCCAGGT
    ATCCAAGGGC CTTCTGGACC TCCAGGAACA AAAGGGGCAC CAGGCGACCC GGGTCCACAA
    GGACCACAGG GACCACCTGG ACCTTTGGGA GAAATTGGCC ATAAGGGACC GCCTGGCCCA
    ATGGGACAAC CTGGCCTCGC AGGTGAACCT GGCATGAAGG GTGATCCTGG CCAGCTGGGA
    GTTCCTGGAG AGCAAGGCCT CATTGGTCAA AGAGGAGAAA TGGGGCTGGA AGGGGACAGT
    GGGCCACCTG GTCCAGACGG AGCCAAGGGA GACAAAGGAG AGCAAGGCCT GGATGGAGAG
    AAAGGAGAAA AAGGCACTGA AGGACTGAAG GGAAAAGAAG GGCCTCCTGG ATATCCCGGC
    ATCACAGGTG TCCGAGGACC AGAAGGGAAA CCAGGAAAAC AGGGGGAAAA GGGCAGGCCT
    GGCCAGAAGG GCAGCAAGGG CTACCAGGGA CAGCTTGGCG AGACAGGACC TCCAGGGAAG
    GAGGGGCCAA AGGGGAACCA AGGCCCTAAA GGATCCCGAG GTACCCTGGG ACCTATGGGT
    GCTCCAGGAA GGATGGGTGC TCAGGGGGAG CCTGGCTTGA AGGGTTACCA GGGGCACGCA
    GGACCACCAG GGCCAATTGG ACCACCGGGG CCGAAGGGTG AGAAGGGAGA ACAAGGTGAT
    GACGGGAAGG TGGAAGGCCC ACAGGGACCA CCCGGAGACA GAGGTCCTGT TGGTGACAGA
    GGAGATCGAG GGGAGCCCGG AGACCCTGGT TATCCTGGCC AAGAAGGGCT TGATGGAGAA
    AGAGGAAAAC CTGGACAGCA AGGCTTACCT GGGGATCCTG GACCACCAGG CCAGCCAGGA
    GCAAAGGGAT CCAAAGGTGA TGTGGGTCAA AAAGGAAAGC AAGGAGCACG AGGCAGTGCA
    GGGAGCAGAG GCTCACCGGG CACCCTTGGG CTACCTGGTC CTCGAGGAGT GGTGGGAAGG
    CAGGGCCAAG AGGGTGCTCC TGGAGTGGAT GGAGTGCCTG GGAAGGACGG CGTGCCTGGG
    GTGCTGGGAG AGCAGGGAGA TGATGGGGAA GAAGGAGTAA CAGGGATGAC AGGCAAAAGA
    GGGAACCCCG GCGTACCGGG ACTGCCCGGG GCACAGGGAC CACCAGGATT CAAGGGTGAA
    AGAGGATTGC CTGGACAGAC TGGCCAAACA GGGAAGCGAG GATCACTGGG AGGAATGGGC
    CTCCCAGGGA GCCCCGGCGA TCCAGGACCC AAAGGCCAGC CGGGAGACTT TGGGGAGGCA
    GGATTCCCAG GGATCGCGGG CATCTTTGGG CCAAAGGGAC CTCCAGGAGA CCCCGGTTTC
    AAAGGCATTC AGGGACCACG TGGCCCACCT GGCATCATGG GAAAAGAAGG AATTATTGGT
    CCACTTGGCA TCCTGGGTCC CACAGGAAGC CCGGGGCCAA AGGGGGACAA AGGCAGCCGC
    GGGGATGTGG GGCTTCGGGG CCCAAGGGGT CCTCCAGGCC CACGAGGACA GCCTGGCCCT
    CCGGGACCGC CGGGATTTCC AGCTTCATTT CAACAAGATG GCTTTGGGGC TGCTTTCCGG
    ACACTGGTTG ACTCCAACGT GGCACTCAAG ACAGAGGGTT ACCAACATCC AGACCTTCTC
    ATGCTGGACC ATGGAGGGGA GATCTTTAAG ACTCTACACT ACCTCAGCAA CCTCATTCAG
    AGCATCAAGA GACCCCTGGG CACCAAGGAA AACCCTGCAC GCATCTGCCG AGACCTCATG
    AACTGTGAAC AGAAGATGAA GGATGGTACC TACTGGATCG ACCCAAATCT CGGCTGTTCC
    TCAGACACCA TACAAGTCAT CTGCAACTTC ACCAACGGTG GGCAGACGTG TCTCAGCCCC
    ATCACCGTCT CCAAGCTGGA TTTTGACATC GGCAGAGTCC AGATGAACTT CCTGCGCCTG
    CTGAGCTCGG AGGTGGTGCA GCACATCACC ATCCACTGCC TGAACACCTC TGTCTGGAGG
    GAGGGCTCTG CTGAGAAACC TTCACAGAAA GCCGTGCGCT TCAAGGCGTG GAACGGGCAA
    ATGTTTGAGG CAGAGGGGCA GCTCAGGCCG GAGGTGGCAG CTGATGATTG CAAGATCAAG
    GATGGACGCT GGCATCGGAC TCTCTTTTCC TTTCGGACGC AGGACACCCA GCAGCTGCCA
    ATCATCAACA TCTACAACCT CCCCCCATCC GAGCCAGGAA AGCATTATCA CCTCGAGGTC
    GGCCCCGTGT GCTTCCTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43620
    Clone ID Related Accession (Same CDS sequence) XM_015279699.2 , XM_015279698.1 , XM_015279699.1
    Accession Version XM_015279699.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGGTCTC
    TGCAGCAAGG ACAGCTGGGC TGTGTCTTTT GGGTCAACTC TTGCTCTAGC CTTTGGCTCA
    AGGTCTCAAA GAACCCGTGA TCTACTGAAC TCAGCAGGTT GTTGGAAGGA GGAATACGAA
    GGACGTAGGG GTAGCGGGTG TCGGTTGGGA CGTGACCAGT GCAAAGGGAT CAGGGGACGA
    GGTGTCGCAC GCGGCTCCAC ACACAAAGCT TTTTCCATGA CCAAAGAAGA CAAGAATGAG
    AGAAACCTCA AGCGACTCGC AGCATGTGGT TTCCCCACCC TCCCCCCCTA TTTTTGCCTT
    ACCCGGTTCC CGAGTTACCA AATGTTCTTC CCATTGAGCA GTAATTCAGA GATGATAGGG
    CTTCCCTCCT GGATGGCCGC GCTGCGCCGG GCTCTCCCAG GCGGAGCTGC AATGCGACGC
    CGGCTGCTCT GCGCACCGGA ACCATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135185.1
    CDS487..6156
    Translation

    Target ORF information:

    RefSeq Version XM_015279699.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279699.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGGTCTC
    TGCAGCAAGG ACAGCTGGGC TGTGTCTTTT GGGTCAACTC TTGCTCTAGC CTTTGGCTCA
    AGGTCTCAAA GAACCCGTGA TCTACTGAAC TCAGCAGGTT GTTGGAAGGA GGAATACGAA
    GGACGTAGGG GTAGCGGGTG TCGGTTGGGA CGTGACCAGT GCAAAGGGAT CAGGGGACGA
    GGTGTCGCAC GCGGCTCCAC ACACAAAGCT TTTTCCATGA CCAAAGAAGA CAAGAATGAG
    AGAAACCTCA AGCGACTCGC AGCATGTGGT TTCCCCACCC TCCCCCCCTA TTTTTGCCTT
    ACCCGGTTCC CGAGTTACCA AATGTTCTTC CCATTGAGCA GTAATTCAGA GATGATAGGG
    CTTCCCTCCT GGATGGCCGC GCTGCGCCGG GCTCTCCCAG GCGGAGCTGC AATGCGACGC
    CGGCTGCTCT GCGCACCGGA ACCATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43620
    Clone ID Related Accession (Same CDS sequence) XM_015279699.2 , XM_015279698.1 , XM_015279699.1
    Accession Version XM_015279698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGGTCTC
    TGCAGCAAGG ACAGCTGGGC TGTGTCTTTT GGGTCAACTC TTGCTCTAGC CTTTGGCTCA
    AGGTCTCAAA GAACCCGTGA TCTACTGAAC TCAGCAGGTT GTTGGAAGGA GGAATACGAA
    GGACGTAGGG GTAGCGGGTG TCGGTTGGGA CGTGACCAGT GCAAAGGGAT CAGGGGACGA
    GGTGTCGCAC GCGGCTCCAC ACACAAAGCT TTTTCCATGA CCAAAGAAGA CAAGAATGAG
    AGAAACCTCA AGCGACTCGC AGCATGTGGT TTCCCCACCC TCCCCCCCTA TTTTTGCCTT
    ACCCGGTTCC CGAGTTACCA AATGTTCTTC CCATTGAGCA GTAATTCAGA GATGATAGGG
    CTTCCCTCCT GGATGGCCGC GCTGCGCCGG GCTCTCCCAG GCGGAGCTGC AATGCGACGC
    CGGCTGCTCT GCGCACCGGA ACCATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135184.1
    CDS488..6157
    Translation

    Target ORF information:

    RefSeq Version XM_015279698.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGGTCTC
    TGCAGCAAGG ACAGCTGGGC TGTGTCTTTT GGGTCAACTC TTGCTCTAGC CTTTGGCTCA
    AGGTCTCAAA GAACCCGTGA TCTACTGAAC TCAGCAGGTT GTTGGAAGGA GGAATACGAA
    GGACGTAGGG GTAGCGGGTG TCGGTTGGGA CGTGACCAGT GCAAAGGGAT CAGGGGACGA
    GGTGTCGCAC GCGGCTCCAC ACACAAAGCT TTTTCCATGA CCAAAGAAGA CAAGAATGAG
    AGAAACCTCA AGCGACTCGC AGCATGTGGT TTCCCCACCC TCCCCCCCTA TTTTTGCCTT
    ACCCGGTTCC CGAGTTACCA AATGTTCTTC CCATTGAGCA GTAATTCAGA GATGATAGGG
    CTTCCCTCCT GGATGGCCGC GCTGCGCCGG GCTCTCCCAG GCGGAGCTGC AATGCGACGC
    CGGCTGCTCT GCGCACCGGA ACCATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43620
    Clone ID Related Accession (Same CDS sequence) XM_015279699.2 , XM_015279698.1 , XM_015279699.1
    Accession Version XM_015279699.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGGTCTC
    TGCAGCAAGG ACAGCTGGGC TGTGTCTTTT GGGTCAACTC TTGCTCTAGC CTTTGGCTCA
    AGGTCTCAAA GAACCCGTGA TCTACTGAAC TCAGCAGGTT GTTGGAAGGA GGAATACGAA
    GGACGTAGGG GTAGCGGGTG TCGGTTGGGA CGTGACCAGT GCAAAGGGAT CAGGGGACGA
    GGTGTCGCAC GCGGCTCCAC ACACAAAGCT TTTTCCATGA CCAAAGAAGA CAAGAATGAG
    AGAAACCTCA AGCGACTCGC AGCATGTGGT TTCCCCACCC TCCCCCCCTA TTTTTGCCTT
    ACCCGGTTCC CGAGTTACCA AATGTTCTTC CCATTGAGCA GTAATTCAGA GATGATAGGG
    CTTCCCTCCT GGATGGCCGC GCTGCGCCGG GCTCTCCCAG GCGGAGCTGC AATGCGACGC
    CGGCTGCTCT GCGCACCGGA ACCATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135185.1
    CDS326..5995
    Translation

    Target ORF information:

    RefSeq Version XM_015279699.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015279699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGGTCTC
    TGCAGCAAGG ACAGCTGGGC TGTGTCTTTT GGGTCAACTC TTGCTCTAGC CTTTGGCTCA
    AGGTCTCAAA GAACCCGTGA TCTACTGAAC TCAGCAGGTT GTTGGAAGGA GGAATACGAA
    GGACGTAGGG GTAGCGGGTG TCGGTTGGGA CGTGACCAGT GCAAAGGGAT CAGGGGACGA
    GGTGTCGCAC GCGGCTCCAC ACACAAAGCT TTTTCCATGA CCAAAGAAGA CAAGAATGAG
    AGAAACCTCA AGCGACTCGC AGCATGTGGT TTCCCCACCC TCCCCCCCTA TTTTTGCCTT
    ACCCGGTTCC CGAGTTACCA AATGTTCTTC CCATTGAGCA GTAATTCAGA GATGATAGGG
    CTTCCCTCCT GGATGGCCGC GCTGCGCCGG GCTCTCCCAG GCGGAGCTGC AATGCGACGC
    CGGCTGCTCT GCGCACCGGA ACCATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa16108
    Clone ID Related Accession (Same CDS sequence) XM_415514.5
    Accession Version XM_415514.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-1(XXVII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_415514.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGTCGGC
    CCCGTGTGCT TCCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_415514.3
    CDS488..5725
    Translation

    Target ORF information:

    RefSeq Version XM_415514.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type XXVII, alpha 1 (COL27A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_415514.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGCCGG AATCAGAGCC GGTCTCCAGA TTCAGAGCTA CAGCAGCAGG CAGGGGGGGA 
    TTCTGCTTTG TCTGGACTGT GTTGTGTTTT AGCTGGTTCC TGGGATTCAC ACAGGGAAAC
    TCTGAAGATG TGGATGTTCT TCAGAGGCTG GGCCTGGGAG GGAGAAGGCC ATCGAGCGGA
    TTGTCCTCAT CACATGCCAT TCCTCAAGGG GTCATTCCTC TCAAATCAGG GGTTATCTTT
    ACTCAGCGAG CACGCGTCGA AGCACCCATC AGCACCATCT TCCCCGCAGG CTCCAGCACT
    GACCTGCTCC TAGTGCTGAG CCTGTGCTCC CACCGCATCA ACAATGCTTT CCTCTTTGCA
    GTCAAGAGCA AAAAGAAAAA ACTGCAGCTG GGGGTTCAGT TTGTGCCTGG GAAGATAATA
    GTGTACGTGG GCCACAAGCA CTCTGTATAT TTTGATTACA ATGTTCACGA TGGCCAGTGG
    CACAATATGG CCATCAATAT CCGTGGCCAG ACAGTCACTT TATTTACTTC TTGTGGGAAG
    CACAGAGTAC ATGCGGATTT GCATTTTAAA AAGGACGAGG CATTGGATCC ACATGGATCT
    TTCCTCTTTG GGAAGATGAA CCATCACTCC GTGCAGTTTG AAGGAGCCAT CTGCCAGTTC
    GATATTTATC CTTCCGCCAA GGCAGCTCAT CATTACTGTA AATACCTGAA AAAACAGTGC
    AGGCAAGCAG ACACCTACCG GCCAAACCTG CCCCCACTCA TCCCCATCCT GCCCAGGGAT
    CCCAGTGCCT CCCCGAGTAC TCCACAGCAT ATGGAGCCCT CGCTTCAGGG TCTGAAAAAC
    TTGACCACTG CCTCCCCATC CTTGGCGCTT GGCAGGGTCA CCGCACCCCT CAAGACTCGG
    GGTTCAGGTG CTACAACTGT TGCCTTGGTC TCGTCCCAGC CGCCGCTGCC AAGACAGACA
    ACCAAAGCCA CAAAGGTTAC AGTGGCCCCA TCTCCCGCTC CATCGAGTGC TGGGACGCAG
    CCGCCCACCC GTTCGCTGCC TCGGGGGACG GTGACGACCC TCAGGGTGTC TCGGCCCACT
    TCCAGCACAC ACACGGAGAA AACTCCCCTG CCTGGTGCCA AAAAGGCCCT GCCAGCAAGC
    CCGAGCCCTG CCACGGCCAG CAGGAAAGAG ACCAAGCAAG AGACCAAAAA GAAGATCAAC
    CAAATACCAA CCACCAAGTC CTCTGAAGCA CCCAGTACTG TAAAGCCTAC CAGGAGGAGG
    GCTGACAACG CAACCAGCAG CGTCCACTTT GTCACCCTGA AGCAAACCCC ACAGACCCCA
    ACCACCGGGC CCCTTCCAAA GAAGCACCTG CCAACCCCCA CCAGGAAAGG GGATCCTGGC
    AATGTCCCTC TGGTGTACAC CAAACCGCCC CTGGGGGCTG CCCGTCCTGT CTCCAGAGCC
    CAGGCCTTCA ACCTCACCAC CCCAGCTGCG ACCGATGGCT ATCAGATCTT TGACCCCCTT
    GGGCCCACTC CGTTTCCATT TTTACTGGGA TACCCAGGAG TGAAAGGAGA CAGAGGACCT
    CAGGGTCCAC CAGGTCTGCC GGGCTTACCA GGACCACCAG GCAAGAGAGG TTCCCGAGGT
    CCTCCGGGTC CCCATGGAAA CCCTGGTTCA CCTGGCCCCC CAGGCCTGAA AGGTCAGAAA
    GGTGATCCTG GGCTGTCGCC TGGAAAAGCT CGCAGAGGAC CAAAGGGAGA CGTGGGCTTA
    CCTGGTCTGA CTGGGTTCCC TGGGCCACCT GGTAGAAAGG GATACAAAGG CTATCCTGGA
    CCACCGGGTC ACCCTGGAGA CCAGGGTGAA CGAGGACCAG CCGGCAGCCC TGGTGCCAAA
    GGTTATCCTG GCAGGCAGGG CTTACCTGGC CCCATAGGGG AAGCTGGTCC AAAAGGCAGT
    CGGGGATATA TTGGTCTTCC GGGATTATTT GGGCTACCAG GGGCTGATGG AGAACGAGGG
    ATCCCTGGAG TTCCTGGGAA GAGGGGCAAG ATGGGTAGGC CGGGTTTTCC TGGTGATTTT
    GGGGAACGAG GACCTCCAGG CATCGACGGG AACCCAGGAG AATTGGGAGC CCCAGGTCCT
    CCTGGAGTCC TTGGACTCAT TGGTGACATG GGCTCTGTTG GACCTATTGG TTATCCAGGA
    CCAAAGGGCT TAAAGGGGCT GATAGGAAAC CCTGGAGAAC ATGGACTGAA AGGTGATAAG
    GGTGATCAGG GAGGAGCAGG TCTTCCAGGA GATATTGGCT TCCAAGGAGA CAAGGGTAGC
    CACGGTTTGC CTGGGGTCCC TGGGGCACGA GGGAAACCAG GCCCTGTGGG AAAAGTTGGT
    GACATAGGTC CGATGGGGTT TCCAGGACCA CCTGGCCCTG AGGGCTTCCC AGGCGACATC
    GGCCCGCCGG GAGAGAACGG CCCCGAAGGC CCAAAGGGGA AGCCGGGAGC GCGGGGTTTG
    CCTGGGCCCA GAGGGCTGCC TGGACAAGAG GGAGATGAAG GACCCTTAGG ACCACCAGGA
    GTCCCTGGTT CAGAGGGTCG ACCTGGCAGG AAGGGATTTC CGGGAAGACC TGGTCCTGAC
    GGTCCCAAGG GCGAGCCAGG AAACCACGGC CGGCCGGGGA AAGTTGGTGA GCAGGGTCTA
    ATGGGATTCA TTGGTCCAGT AGGGGAGCCT GGAATAGTGG GAGAAAAGGG TGACCGTGGC
    TCAGTGGGAC CCCCAGGCCC TCCTGGAGTC AAGGGGTCGA TGGGGCACCC TGGGATACCA
    GGAGCAGTGG GTTTTCCCGG CATCCCCGGA CCAGCAGGTC CGGCAGGAGC CCGCGGTGCA
    CCAGGGATCA GAGGGGTGAA AGGCCGCCGG GGTGCCAGAG GGCCTGATGG ACCAGTAGGC
    GAGCCGGGGT CACGAGGTGC CAAGGGCCGT CCAGGGGAGC CAGGCAAGCT GGGGCCAGAT
    GGGCTGCAGG GGAAGATGGG CGAGACTGGG GAGACAGGAT CCCGTGGCTT TCCAGGTATC
    CAAGGGCCTT CTGGACCTCC AGGAACAAAA GGGGCACCAG GCGACCCGGG TCCACAAGGA
    CCACAGGGAC CACCTGGACC TTTGGGAGAA ATTGGCCATA AGGGACCGCC TGGCCCAATG
    GGACAACCTG GCCTCGCAGG TGAACCTGGC ATGAAGGGTG ATCCTGGCCA GCTGGGAGTT
    CCTGGAGAGC AAGGCCTCAT TGGTCAAAGA GGAGAAATGG GGCTGGAAGG GGACAGTGGG
    CCACCTGGTC CAGACGGAGC CAAGGGAGAC AAAGGAGAGC AAGGCCTGGA TGGAGAGAAA
    GGAGAAAAAG GCACTGAAGG ACTGAAGGGA AAAGAAGGGC CTCCTGGATA TCCCGGCATC
    ACAGGTGTCC GAGGACCAGA AGGGAAACCA GGAAAACAGG GGGAAAAGGG CAGGCCTGGC
    CAGAAGGGCA GCAAGGGCTA CCAGGGACAG CTTGGCGAGA CAGGACCTCC AGGGAAGGAG
    GGGCCAAAGG GGAACCAAGG CCCTAAAGGA TCCCGAGGTA CCCTGGGACC TATGGGTGCT
    CCAGGAAGGA TGGGTGCTCA GGGGGAGCCT GGCTTGAAGG GTTACCAGGG GCACGCAGGA
    CCACCAGGGC CAATTGGACC ACCGGGGCCG AAGGGTGAGA AGGGAGAACA AGGTGATGAC
    GGGAAGGTGG AAGGCCCACA GGGACCACCC GGAGACAGAG GTCCTGTTGG TGACAGAGGA
    GATCGAGGGG AGCCCGGAGA CCCTGGTTAT CCTGGCCAAG AAGGGCTTGA TGGAGAAAGA
    GGAAAACCTG GACAGCAAGG CTTACCTGGG GATCCTGGAC CACCAGGCCA GCCAGGAGCA
    AAGGGATCCA AAGGTGATGT GGGTCAAAAA GGAAAGCAAG GAGCACGAGG CAGTGCAGGG
    AGCAGAGGCT CACCGGGCAC CCTTGGGCTA CCTGGTCCTC GAGGAGTGGT GGGAAGGCAG
    GGCCAAGAGG GTGCTCCTGG AGTGGATGGA GTGCCTGGGA AGGACGGCGT GCCTGGGGTG
    CTGGGAGAGC AGGGAGATGA TGGGGAAGAA GGAGTAACAG GGATGACAGG CAAAAGAGGG
    AACCCCGGCG TACCGGGACT GCCCGGGGCA CAGGGACCAC CAGGATTCAA GGGTGAAAGA
    GGATTGCCTG GACAGACTGG CCAAACAGGG AAGCGAGGAT CACTGGGAGG AATGGGCCTC
    CCAGGGAGCC CCGGCGATCC AGGACCCAAA GGCCAGCCGG GAGACTTTGG GGAGGCAGGA
    TTCCCAGGGA TCGCGGGCAT CTTTGGGCCA AAGGGACCTC CAGGAGACCC CGGTTTCAAA
    GGCATTCAGG GACCACGTGG CCCACCTGGC ATCATGGGAA AAGAAGGAAT TATTGGTCCA
    CTTGGCATCC TGGGTCCCAC AGGAAGCCCG GGGCCAAAGG GGGACAAAGG CAGCCGCGGG
    GATGTGGGGC TTCGGGGCCC AAGGGGTCCT CCAGGCCCAC GAGGACAGCC TGGCCCTCCG
    GGACCGCCGG GATTTCCAGC TTCATTTCAA CAAGATGGCT TTGGGGCTGC TTTCCGGACA
    CTGGTTGACT CCAACGTGGC ACTCAAGACA GAGGGTTACC AACATCCAGA CCTTCTCATG
    CTGGACCATG GAGGGGAGAT CTTTAAGACT CTACACTACC TCAGCAACCT CATTCAGAGC
    ATCAAGAGAC CCCTGGGCAC CAAGGAAAAC CCTGCACGCA TCTGCCGAGA CCTCATGAAC
    TGTGAACAGA AGATGAAGGA TGGTACCTAC TGGATCGACC CAAATCTCGG CTGTTCCTCA
    GACACCATAC AAGTCATCTG CAACTTCACC AACGGTGGGC AGACGTGTCT CAGCCCCATC
    ACCGTCTCCA AGCTGGATTT TGACATCGGC AGAGTCCAGA TGAACTTCCT GCGCCTGCTG
    AGCTCGGAGG TGGTGCAGCA CATCACCATC CACTGCCTGA ACACCTCTGT CTGGAGGGAG
    GGCTCTGCTG AGAAACCTTC ACAGAAAGCC GTGCGCTTCA AGGCGTGGAA CGGGCAAATG
    TTTGAGGCAG AGGGGCAGCT CAGGCCGGAG GTGGCAGCTG ATGATTGCAA GATCAAGGAT
    GGACGCTGGC ATCGGACTCT CTTTTCCTTT CGGACGCAGG ACACCCAGCA GCTGCCAATC
    ATCAACATCT ACAACCTCCC CCCATCCGAG CCAGGAAAGC ATTATCACCT CGAGGTCGGC
    CCCGTGTGCT TCCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.