PCF11 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCF11
Entrez Gene ID	419021
Full Name	PCF11 cleavage and polyadenylation factor subunit
Synonyms	2500001H09RIK
General protein information	Preferred Names PCF11 cleavage and polyadenylation factor subunit Names pre-mRNA cleavage complex 2 protein Pcf11 PCF11, cleavage and polyadenylation factor subunit, homolog pre-mRNA cleavage complex II protein Pcf11
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025146901.1	XP_025002669.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform X4
XM_004938893.2	XP_004938950.2	pre-mRNA cleavage complex 2 protein Pcf11 isoform X3
XM_004938892.3	XP_004938949.3	pre-mRNA cleavage complex 2 protein Pcf11 isoform X2
XM_004938893.3	XP_004938950.3	pre-mRNA cleavage complex 2 protein Pcf11 isoform X3
XM_004938891.2	XP_004938948.2	pre-mRNA cleavage complex 2 protein Pcf11 isoform X1
XM_004938892.2	XP_004938949.2	pre-mRNA cleavage complex 2 protein Pcf11 isoform X2
XM_004938891.3	XP_004938948.3	pre-mRNA cleavage complex 2 protein Pcf11 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PCF11	XP_508673.4
	PCF11	XP_002808130.1
Gallus gallus (chicken)	PCF11	XP_417214.3
Danio rerio (zebrafish)	pcf11	NP_001007308.1
Homo sapiens (human)	PCF11	NP_056969.2
Bos taurus (cattle)	LOC510604	XP_002699113.2
Canis lupus familiaris (dog)	PCF11	XP_533992.4
Mus musculus (house mouse)	Pcf11	NP_083354.3
Rattus norvegicus (Norway rat)	Pcf11	XP_341884.4

Gene Ontology

The following PCF11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCF11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa143225	XM_004938891.3 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa143226	XM_004938892.3 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa143227	XM_004938893.3 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa143228	XM_025146901.1 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa31099	XM_004938891.2 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa31100	XM_004938892.2 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa31101	XM_004938893.2 Latest version!	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCF11 ( XM_004938891.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004938948.3 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa143225
Clone ID Related Accession (Same CDS sequence)	XM_004938891.3
Accession Version	XM_004938891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004938891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGTGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC GCCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACAA GCGAATGAAA CATCTGCACA GCCAGCTGCT 
GAAGAGGAGG ATGATGACCA GGGTGATCAT GATGTCCCAG ACCTCACAAA CTTTAAAGTT 
GAAGAACTCA GACAGCGTTA TGATAGTGTT ATAAATCGAC TATACACTGG AATTCAGTGC 
TACTCGTGTG GAATGAGATT TACTACTTCA CAGACAGACG TTTATGCAGA CCATTTAGAT 
TGGCACTATC GCCAGAACCG TTCGGAAAAA GATGTTAGCA GAAAAATTAC ACACAGAAGG 
TGGTACTACA GCGTAACGGA TTGGATTGAG TTTGAAGAAA TAGCTGACTT AGAGGAGCGT 
GCAAAAAGCC AATTTTTTGA AAAAGCTCAT GAAGAAGTTG TACTGAAGAC ACAGGAAGCT 
GCAAAAGAGA AGGAGTTTCA GAGTGTCCCT GCTGGACCAG CTGGAGCAGT TGAGAGGTGT 
GAAATCTGCC GGGAGCAATT TGAGCAGTAC TGGGATGAGG AAGAGGAGGA GTGGCATCTA 
AAGAATGCTA TCAGAATGCA TGAAAAGATT TACCATCCAT CCTGTTATGA GGATTACCAA 
AATACATCAT CATTTGATTG CACACCCTCT CCCAGCAAGA CCCCTGTAGA AAATCCCCTA 
AATATTATGT TGAACATTGT TAAACAGGAA ACAGATGAGT CCTGTGACTC ACCTCAAGTG 
AAAGAGGAAC CTGATGACAC CCCTGCTGCC TGTGCAGAAG AGAGCACACC TGCATCTATA 
GATGTTAAAA CAGAACCTGA TGAGTCTGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004938948.3
CDS	583..5655
Translation	MSAPESSAGSSEAREDACRDYQSSLEDLTFNSKPHINMLTILAEENVPFAKDIVSLIEAQIAKAPASEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDVTTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAQANETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_004938891.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004938891.3

ORF Insert Sequence:

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGTGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC GCCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACAA GCGAATGAAA CATCTGCACA GCCAGCTGCT 
GAAGAGGAGG ATGATGACCA GGGTGATCAT GATGTCCCAG ACCTCACAAA CTTTAAAGTT 
GAAGAACTCA GACAGCGTTA TGATAGTGTT ATAAATCGAC TATACACTGG AATTCAGTGC 
TACTCGTGTG GAATGAGATT TACTACTTCA CAGACAGACG TTTATGCAGA CCATTTAGAT 
TGGCACTATC GCCAGAACCG TTCGGAAAAA GATGTTAGCA GAAAAATTAC ACACAGAAGG 
TGGTACTACA GCGTAACGGA TTGGATTGAG TTTGAAGAAA TAGCTGACTT AGAGGAGCGT 
GCAAAAAGCC AATTTTTTGA AAAAGCTCAT GAAGAAGTTG TACTGAAGAC ACAGGAAGCT 
GCAAAAGAGA AGGAGTTTCA GAGTGTCCCT GCTGGACCAG CTGGAGCAGT TGAGAGGTGT 
GAAATCTGCC GGGAGCAATT TGAGCAGTAC TGGGATGAGG AAGAGGAGGA GTGGCATCTA 
AAGAATGCTA TCAGAATGCA TGAAAAGATT TACCATCCAT CCTGTTATGA GGATTACCAA 
AATACATCAT CATTTGATTG CACACCCTCT CCCAGCAAGA CCCCTGTAGA AAATCCCCTA 
AATATTATGT TGAACATTGT TAAACAGGAA ACAGATGAGT CCTGTGACTC ACCTCAAGTG 
AAAGAGGAAC CTGATGACAC CCCTGCTGCC TGTGCAGAAG AGAGCACACC TGCATCTATA 
GATGTTAAAA CAGAACCTGA TGAGTCTGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( XM_004938892.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004938949.3 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X2, mRNA.

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CloneID	OGa143226
Clone ID Related Accession (Same CDS sequence)	XM_004938892.3
Accession Version	XM_004938892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004938892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGTGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC GCCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACCG AATGAAACAT CTGCACAGCC AGCTGCTGAA 
GAGGAGGATG ATGACCAGGG TGATCATGAT GTCCCAGACC TCACAAACTT TAAAGTTGAA 
GAACTCAGAC AGCGTTATGA TAGTGTTATA AATCGACTAT ACACTGGAAT TCAGTGCTAC 
TCGTGTGGAA TGAGATTTAC TACTTCACAG ACAGACGTTT ATGCAGACCA TTTAGATTGG 
CACTATCGCC AGAACCGTTC GGAAAAAGAT GTTAGCAGAA AAATTACACA CAGAAGGTGG 
TACTACAGCG TAACGGATTG GATTGAGTTT GAAGAAATAG CTGACTTAGA GGAGCGTGCA 
AAAAGCCAAT TTTTTGAAAA AGCTCATGAA GAAGTTGTAC TGAAGACACA GGAAGCTGCA 
AAAGAGAAGG AGTTTCAGAG TGTCCCTGCT GGACCAGCTG GAGCAGTTGA GAGGTGTGAA 
ATCTGCCGGG AGCAATTTGA GCAGTACTGG GATGAGGAAG AGGAGGAGTG GCATCTAAAG 
AATGCTATCA GAATGCATGA AAAGATTTAC CATCCATCCT GTTATGAGGA TTACCAAAAT 
ACATCATCAT TTGATTGCAC ACCCTCTCCC AGCAAGACCC CTGTAGAAAA TCCCCTAAAT 
ATTATGTTGA ACATTGTTAA ACAGGAAACA GATGAGTCCT GTGACTCACC TCAAGTGAAA 
GAGGAACCTG ATGACACCCC TGCTGCCTGT GCAGAAGAGA GCACACCTGC ATCTATAGAT 
GTTAAAACAG AACCTGATGA GTCTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004938949.3
CDS	585..5654
Translation	MSAPESSAGSSEAREDACRDYQSSLEDLTFNSKPHINMLTILAEENVPFAKDIVSLIEAQIAKAPASEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDVTTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAPNETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_004938892.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004938892.3

ORF Insert Sequence:

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGTGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC GCCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACCG AATGAAACAT CTGCACAGCC AGCTGCTGAA 
GAGGAGGATG ATGACCAGGG TGATCATGAT GTCCCAGACC TCACAAACTT TAAAGTTGAA 
GAACTCAGAC AGCGTTATGA TAGTGTTATA AATCGACTAT ACACTGGAAT TCAGTGCTAC 
TCGTGTGGAA TGAGATTTAC TACTTCACAG ACAGACGTTT ATGCAGACCA TTTAGATTGG 
CACTATCGCC AGAACCGTTC GGAAAAAGAT GTTAGCAGAA AAATTACACA CAGAAGGTGG 
TACTACAGCG TAACGGATTG GATTGAGTTT GAAGAAATAG CTGACTTAGA GGAGCGTGCA 
AAAAGCCAAT TTTTTGAAAA AGCTCATGAA GAAGTTGTAC TGAAGACACA GGAAGCTGCA 
AAAGAGAAGG AGTTTCAGAG TGTCCCTGCT GGACCAGCTG GAGCAGTTGA GAGGTGTGAA 
ATCTGCCGGG AGCAATTTGA GCAGTACTGG GATGAGGAAG AGGAGGAGTG GCATCTAAAG 
AATGCTATCA GAATGCATGA AAAGATTTAC CATCCATCCT GTTATGAGGA TTACCAAAAT 
ACATCATCAT TTGATTGCAC ACCCTCTCCC AGCAAGACCC CTGTAGAAAA TCCCCTAAAT 
ATTATGTTGA ACATTGTTAA ACAGGAAACA GATGAGTCCT GTGACTCACC TCAAGTGAAA 
GAGGAACCTG ATGACACCCC TGCTGCCTGT GCAGAAGAGA GCACACCTGC ATCTATAGAT 
GTTAAAACAG AACCTGATGA GTCTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( XM_004938893.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004938950.3 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa143227
Clone ID Related Accession (Same CDS sequence)	XM_004938893.3
Accession Version	XM_004938893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004938893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG TGGATGAAAA TACTAGGAAA AGTTTATTTA AGTTACGCTC CACATGGGAC 
GATATTTTTC CTTTGAAGAA ACTATATGCA CTTGACGTCA GAGTCAACTC ATTAGATCCT 
GCTTGGCCAA TTAAACCTCT GCCCCCAAAT GTGAATACTT CTAGCATCCA TGTGAATCCT 
AAGTTTTTAA ATAAATCGCC AGAGGAATCT ACTGCATCTA CCTCTGCTGT CACTTCTGGT 
GCCTCTACTC CTCCAGCTGT TCCTGAAATA CAAAAGAATT TGACACAGGA GCAACTGATA 
AGGCAGCAAT TGCTTGCGAA GCAAAAACAG TTGTTAGAAC TTCAGCAAAA AAAGTTAGAG 
TTGGAGTTGG AACAGACTAA AGCACAGTTG GCTGTTTCTC TAAGTGTTCA GCAAGGATCA 
TCTTCTATAC CTTCAGTTCC AGCACCTTCC AAGCCACATG TGTCCCAGAC ACCTCACATG 
CCAGTTAAAA CACCTACTCA GGCTTCACTG CAATCTGAAA AGATCAAACC ATCCCCAAGT 
CCTCCACTTC ACGATGTCAA AATAGTAAAC AGGGATCCTC GACTTAATAG AATGTCGCAA 
CATTCTTCTC ATGCTAAAGA TCAGTCTCAT AGGAAAGAAT TTCCACCAAA CACAACTGGT 
CAGTCTGATA CCAAGGCAAG CAAAACATCC CAGTCTGAAA AACAGAGCTC AACAAAACAA 
GAGAAAGTGA AAGCTGGTGA AAAAACACAG AAGAAAGAAC CCGATCAATT GGAAACAAAG 
TCGAAATCAC CATCTCCTTT GAAAAACAAG CTTCCGAATA CTAAAGATAC TAAAACCCAA 
GAATCTGAAA GCACAAAAGT ATCTGAAATG AGTAAACGGG ATCCAAGACT GAAAAAGCAT 
CTTCAAGAGA AACCAGAGAG CAAAGGGGAT GAGGTGAAAG AAAAGAGGAG AAATACAGAG 
AAAAAAGAAA AAGACGAACA TAAGACAGGT GAACATAGGC CAGTAGGCAG CAGAAATAAA 
ACAGTTAATG GTGTTGTTCA GAAACAAGAC GTGACTACAG AAGAATCGGA AAAACAGGGT 
GGAAAACAAG GGAGATCCAG TAATAGAAAA CGGTCACGAT CCCGCTCTCC TAAGTCACGG 
TCACCATCTA CACACTCTCC AAAACGAAGA GAGAGGAGAT CACCCAAAAG AAGACTCAGG 
AGTTTATCTC CGACTTCTTC AACTCCTAAA ATTGGAAAGA TACGTCAGAT AGGTCCTAAG 
CAGTCTCATA CTGAAGAAGC CTCACAAGGG GCAAGAGATG AAAGAAATTC TAATAAGCGA 
AATGTTAAAC AAGAGGTGCG AGATCCAAGG AGAGTGAAAA AAAGCCAAGA AGACAGACCC 
CAAGAAATAG CAAGCCAGCA TTCTACAAAA GCTTCTCCCG ATCCAAAGGA GAATGCAGAG 
AATTGGCAGG GATCCAAAGC AGGCAAGAGG TGGAAATCCG GTTGGGAGGA AAATAAAAAC 
TCGCAGCAGA CTGAAGAACA CCAAGCGGCT GGTAAATCCC CTCACCAAAG ACACCGAGAA 
AACTGGCCTG CTGCTAAAGG AATTCTGTCA CCTCGTGCAC CAAAACAGCA GCACCGGTTA 
AGCGTGGATG CCAATTTACA AATTCCCAAA GAACTGACAT CTGCAAGCAA AAGAGAATTA 
CTTAAAAAGG CCAATGACCG CTTAGCATCT GGTGAAATAA CCCAAGATGA GTTTCTTGTG 
GTAGCTCATC AGATTCGACA GTTATTCCAG TACCAGGAAG GAAAGCACAG ATGTAATGTG 
TGGGATAGCC CTACAGAGGA AAAAGGCTCT TTGAAAAGGA AGCCTCTTCT ATCTGATGCA 
GAATTAACAT ACTATGAGCA TAAAGCTAAA CTGAAAAGAA CACAAGTTCA GCATTCATTG 
TCAAGACTTG ATCTCTTGGA TCCTGATGAT ATTTTGGATT ATCGTGTACC AGACGCGTTG 
CTTTCTGGAA TAGACTGTGA GCAAGCGAAA GCAAAGCGTG GAGTACAGTT TGAAAGAAAA 
GAACCTTTTG GAGAAAGATC AAGACGGCAC TCTCCTGTAA GTGGAACAAG CAGACCCTTT 
GCTGATAACC TTTCGCCGCT TGAGGGTCGA CGAAGGCTTG AGGAACAAAA TGCTACTAAA 
GGAAGCAGGG GTTCGAAAAA CTTCAATCCT TATGACAACT GGGGAGAATC GGATGAATTC 
AGAGATGCTC TCAGACAGCA GGGAAAAGGT ACAGCAGAGT TCCAGAAGAT AGATGGTGAT 
GCCGTCTGTA GGTTTGATAA CCGTGAAGAA AGACAGCTGC TTGGGAAAGC TGGTGTTCGA 
GATGAACCAA GATCACCATT CAATGAACGT TTCAAAAGAG CTAGATACGA TGATCCAGAT 
AAAGAACCAT TTCCAGACAG TCCAGGGTCA AGATTTGGAG GCATAGAAGC AAAGCCAAGA 
ATAAGTGCAC TAATGGAAGA CAGACCTTTA TTTGATGGCT CATCTAGACC ATCTGCTGCA 
AGAGTTGGGG CAGATGGACA AGGGAGTCCT TTTATTGATA GTCCTACTGC TGGCTCAAGT 
TCTAGAATTG ATGGGCCACC TGGACAGGCT GCTCTGAGAT TTGAGGGGCC TTTGGTGGGG 
GCAGGTGCTT CTCAGTTTGA TGGACCCCTG GCAGGAGCAG GGGGAGCTGG AGCCCTGAGA 
TTTGATGGGC CACCAGGGGC GCTCAGGTTT GAAGGACCAC AGGGACAGGT TGGTGGGGGA 
GGTCCTCTGA GGTTTGAGGG GCCTATTGGG CAGATAGGTG GGCCATTGCG GTTTGAGGGG 
CCTGCAGGCC AGCCTGTGGG TGGTCCTAGA TTTGAAGGAC CTGGAGTTGG TCTCAGGTTT 
GAGGGACCCC GTGGTCAGCC TTCAGGTGGT CTCAGGTTTG AGGGACCTCA TGGTCAACCT 
CTGGGGCCTC GAGGTCAGCC AGGGGGTGGT CTCAGGTTTG AGGGACCGCA CGGTCAGCCC 
TTGGGGCCTC ACGGTCAAGC AGGAGGTGGT CTCAGGTTTG AGGGTCCGCA TTTACAGCCA 
TTGGGGCCCC ATGGTCAACC TGGAGGTGGC CTCAGATTTG AGGGGCCACA TGGACAGCCT 
ATGGGGCCAC ATGGACCGTC AGGAGGTGGG CTCAGATTTG AGGGGCCACA TGGACCGCCA 
GGTGTAGGGC CGAGGTTGAT TGATGGACCA GTGCACCAGG GAGTTGGTGG ACTTAGATTT 
GATGGTCCTC TGGGGCGGTC TGGTCCGAGA TTTGATGGCT GTCATGCTGG CTTTGATGGT 
CAACCTGGAC AGCTGTCTCT TTTGCAAAGA TTTGATGGAA TTCATGGACA GCCTGGCCCA 
AGATTTGAAA GGGCTCCTGG CCAGCCCGCA CAACCGCGAT TTGATGCAGC CATACCTCAA 
AGATTTGATG GTCCTCACCA GCCAGCCTCG AGATTTGACC TGCCTCTTGG ACTTCACGGT 
GCACGTTTTG AAAATATAGC TAGCCATCCT GCCTCACGAT TGGAGATGTC GCCATATGGA 
CAAAGTGGTC CGTTTGTTGA CCATCCCGGA CAGGGTTTCA GCGGACCAGC TCATGGAATG 
CAGTTCCAAA GACCTGAAAT GTTTGACAGT TCACTAGGAC CAAATTTCAG TGGGCCAGCT 
GGCCCAGGAG GACAGAATTT TTCATTGAGA GCATCTGGAC ATTATTTCGA TGACAAACTT 
CAGGGTCCTC AATACGGAAA CTTCAATAGT ATGTCAATGG GAGGTCATCA GGTTTCGCAC 
ATGTCTGCTC AGCCAGGACC TTATGGCCAA GGTCAACAGT ATTTACCAAA TCCTGGAAGC 
TTTGTTCAGA ACCCTGCAGG AGGAGCCCTT CCACATTCAT ATCCTGATAA CCACCTTGGT 
CAGCTTGATG TCAATGAATT GTTTGCCAAA CTGTTGTCAA CAGGAATCCT CAAACTGTCA 
AAAACAGATT CCACTTCAGC ACAAGCGAAT GAAACATCTG CACAGCCAGC TGCTGAAGAG 
GAGGATGATG ACCAGGGTGA TCATGATGTC CCAGACCTCA CAAACTTTAA AGTTGAAGAA 
CTCAGACAGC GTTATGATAG TGTTATAAAT CGACTATACA CTGGAATTCA GTGCTACTCG 
TGTGGAATGA GATTTACTAC TTCACAGACA GACGTTTATG CAGACCATTT AGATTGGCAC 
TATCGCCAGA ACCGTTCGGA AAAAGATGTT AGCAGAAAAA TTACACACAG AAGGTGGTAC 
TACAGCGTAA CGGATTGGAT TGAGTTTGAA GAAATAGCTG ACTTAGAGGA GCGTGCAAAA 
AGCCAATTTT TTGAAAAAGC TCATGAAGAA GTTGTACTGA AGACACAGGA AGCTGCAAAA 
GAGAAGGAGT TTCAGAGTGT CCCTGCTGGA CCAGCTGGAG CAGTTGAGAG GTGTGAAATC 
TGCCGGGAGC AATTTGAGCA GTACTGGGAT GAGGAAGAGG AGGAGTGGCA TCTAAAGAAT 
GCTATCAGAA TGCATGAAAA GATTTACCAT CCATCCTGTT ATGAGGATTA CCAAAATACA 
TCATCATTTG ATTGCACACC CTCTCCCAGC AAGACCCCTG TAGAAAATCC CCTAAATATT 
ATGTTGAACA TTGTTAAACA GGAAACAGAT GAGTCCTGTG ACTCACCTCA AGTGAAAGAG 
GAACCTGATG ACACCCCTGC TGCCTGTGCA GAAGAGAGCA CACCTGCATC TATAGATGTT 
AAAACAGAAC CTGATGAGTC TGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004938950.3
CDS	582..5528
Translation	MSAPESSAGSSEAREDACRDYQSSLEDLTFNSKPHINMLTILAEENVPFAKDIVSLIEAQIAKVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDVTTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAQANETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_004938893.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004938893.3

ORF Insert Sequence:

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG TGGATGAAAA TACTAGGAAA AGTTTATTTA AGTTACGCTC CACATGGGAC 
GATATTTTTC CTTTGAAGAA ACTATATGCA CTTGACGTCA GAGTCAACTC ATTAGATCCT 
GCTTGGCCAA TTAAACCTCT GCCCCCAAAT GTGAATACTT CTAGCATCCA TGTGAATCCT 
AAGTTTTTAA ATAAATCGCC AGAGGAATCT ACTGCATCTA CCTCTGCTGT CACTTCTGGT 
GCCTCTACTC CTCCAGCTGT TCCTGAAATA CAAAAGAATT TGACACAGGA GCAACTGATA 
AGGCAGCAAT TGCTTGCGAA GCAAAAACAG TTGTTAGAAC TTCAGCAAAA AAAGTTAGAG 
TTGGAGTTGG AACAGACTAA AGCACAGTTG GCTGTTTCTC TAAGTGTTCA GCAAGGATCA 
TCTTCTATAC CTTCAGTTCC AGCACCTTCC AAGCCACATG TGTCCCAGAC ACCTCACATG 
CCAGTTAAAA CACCTACTCA GGCTTCACTG CAATCTGAAA AGATCAAACC ATCCCCAAGT 
CCTCCACTTC ACGATGTCAA AATAGTAAAC AGGGATCCTC GACTTAATAG AATGTCGCAA 
CATTCTTCTC ATGCTAAAGA TCAGTCTCAT AGGAAAGAAT TTCCACCAAA CACAACTGGT 
CAGTCTGATA CCAAGGCAAG CAAAACATCC CAGTCTGAAA AACAGAGCTC AACAAAACAA 
GAGAAAGTGA AAGCTGGTGA AAAAACACAG AAGAAAGAAC CCGATCAATT GGAAACAAAG 
TCGAAATCAC CATCTCCTTT GAAAAACAAG CTTCCGAATA CTAAAGATAC TAAAACCCAA 
GAATCTGAAA GCACAAAAGT ATCTGAAATG AGTAAACGGG ATCCAAGACT GAAAAAGCAT 
CTTCAAGAGA AACCAGAGAG CAAAGGGGAT GAGGTGAAAG AAAAGAGGAG AAATACAGAG 
AAAAAAGAAA AAGACGAACA TAAGACAGGT GAACATAGGC CAGTAGGCAG CAGAAATAAA 
ACAGTTAATG GTGTTGTTCA GAAACAAGAC GTGACTACAG AAGAATCGGA AAAACAGGGT 
GGAAAACAAG GGAGATCCAG TAATAGAAAA CGGTCACGAT CCCGCTCTCC TAAGTCACGG 
TCACCATCTA CACACTCTCC AAAACGAAGA GAGAGGAGAT CACCCAAAAG AAGACTCAGG 
AGTTTATCTC CGACTTCTTC AACTCCTAAA ATTGGAAAGA TACGTCAGAT AGGTCCTAAG 
CAGTCTCATA CTGAAGAAGC CTCACAAGGG GCAAGAGATG AAAGAAATTC TAATAAGCGA 
AATGTTAAAC AAGAGGTGCG AGATCCAAGG AGAGTGAAAA AAAGCCAAGA AGACAGACCC 
CAAGAAATAG CAAGCCAGCA TTCTACAAAA GCTTCTCCCG ATCCAAAGGA GAATGCAGAG 
AATTGGCAGG GATCCAAAGC AGGCAAGAGG TGGAAATCCG GTTGGGAGGA AAATAAAAAC 
TCGCAGCAGA CTGAAGAACA CCAAGCGGCT GGTAAATCCC CTCACCAAAG ACACCGAGAA 
AACTGGCCTG CTGCTAAAGG AATTCTGTCA CCTCGTGCAC CAAAACAGCA GCACCGGTTA 
AGCGTGGATG CCAATTTACA AATTCCCAAA GAACTGACAT CTGCAAGCAA AAGAGAATTA 
CTTAAAAAGG CCAATGACCG CTTAGCATCT GGTGAAATAA CCCAAGATGA GTTTCTTGTG 
GTAGCTCATC AGATTCGACA GTTATTCCAG TACCAGGAAG GAAAGCACAG ATGTAATGTG 
TGGGATAGCC CTACAGAGGA AAAAGGCTCT TTGAAAAGGA AGCCTCTTCT ATCTGATGCA 
GAATTAACAT ACTATGAGCA TAAAGCTAAA CTGAAAAGAA CACAAGTTCA GCATTCATTG 
TCAAGACTTG ATCTCTTGGA TCCTGATGAT ATTTTGGATT ATCGTGTACC AGACGCGTTG 
CTTTCTGGAA TAGACTGTGA GCAAGCGAAA GCAAAGCGTG GAGTACAGTT TGAAAGAAAA 
GAACCTTTTG GAGAAAGATC AAGACGGCAC TCTCCTGTAA GTGGAACAAG CAGACCCTTT 
GCTGATAACC TTTCGCCGCT TGAGGGTCGA CGAAGGCTTG AGGAACAAAA TGCTACTAAA 
GGAAGCAGGG GTTCGAAAAA CTTCAATCCT TATGACAACT GGGGAGAATC GGATGAATTC 
AGAGATGCTC TCAGACAGCA GGGAAAAGGT ACAGCAGAGT TCCAGAAGAT AGATGGTGAT 
GCCGTCTGTA GGTTTGATAA CCGTGAAGAA AGACAGCTGC TTGGGAAAGC TGGTGTTCGA 
GATGAACCAA GATCACCATT CAATGAACGT TTCAAAAGAG CTAGATACGA TGATCCAGAT 
AAAGAACCAT TTCCAGACAG TCCAGGGTCA AGATTTGGAG GCATAGAAGC AAAGCCAAGA 
ATAAGTGCAC TAATGGAAGA CAGACCTTTA TTTGATGGCT CATCTAGACC ATCTGCTGCA 
AGAGTTGGGG CAGATGGACA AGGGAGTCCT TTTATTGATA GTCCTACTGC TGGCTCAAGT 
TCTAGAATTG ATGGGCCACC TGGACAGGCT GCTCTGAGAT TTGAGGGGCC TTTGGTGGGG 
GCAGGTGCTT CTCAGTTTGA TGGACCCCTG GCAGGAGCAG GGGGAGCTGG AGCCCTGAGA 
TTTGATGGGC CACCAGGGGC GCTCAGGTTT GAAGGACCAC AGGGACAGGT TGGTGGGGGA 
GGTCCTCTGA GGTTTGAGGG GCCTATTGGG CAGATAGGTG GGCCATTGCG GTTTGAGGGG 
CCTGCAGGCC AGCCTGTGGG TGGTCCTAGA TTTGAAGGAC CTGGAGTTGG TCTCAGGTTT 
GAGGGACCCC GTGGTCAGCC TTCAGGTGGT CTCAGGTTTG AGGGACCTCA TGGTCAACCT 
CTGGGGCCTC GAGGTCAGCC AGGGGGTGGT CTCAGGTTTG AGGGACCGCA CGGTCAGCCC 
TTGGGGCCTC ACGGTCAAGC AGGAGGTGGT CTCAGGTTTG AGGGTCCGCA TTTACAGCCA 
TTGGGGCCCC ATGGTCAACC TGGAGGTGGC CTCAGATTTG AGGGGCCACA TGGACAGCCT 
ATGGGGCCAC ATGGACCGTC AGGAGGTGGG CTCAGATTTG AGGGGCCACA TGGACCGCCA 
GGTGTAGGGC CGAGGTTGAT TGATGGACCA GTGCACCAGG GAGTTGGTGG ACTTAGATTT 
GATGGTCCTC TGGGGCGGTC TGGTCCGAGA TTTGATGGCT GTCATGCTGG CTTTGATGGT 
CAACCTGGAC AGCTGTCTCT TTTGCAAAGA TTTGATGGAA TTCATGGACA GCCTGGCCCA 
AGATTTGAAA GGGCTCCTGG CCAGCCCGCA CAACCGCGAT TTGATGCAGC CATACCTCAA 
AGATTTGATG GTCCTCACCA GCCAGCCTCG AGATTTGACC TGCCTCTTGG ACTTCACGGT 
GCACGTTTTG AAAATATAGC TAGCCATCCT GCCTCACGAT TGGAGATGTC GCCATATGGA 
CAAAGTGGTC CGTTTGTTGA CCATCCCGGA CAGGGTTTCA GCGGACCAGC TCATGGAATG 
CAGTTCCAAA GACCTGAAAT GTTTGACAGT TCACTAGGAC CAAATTTCAG TGGGCCAGCT 
GGCCCAGGAG GACAGAATTT TTCATTGAGA GCATCTGGAC ATTATTTCGA TGACAAACTT 
CAGGGTCCTC AATACGGAAA CTTCAATAGT ATGTCAATGG GAGGTCATCA GGTTTCGCAC 
ATGTCTGCTC AGCCAGGACC TTATGGCCAA GGTCAACAGT ATTTACCAAA TCCTGGAAGC 
TTTGTTCAGA ACCCTGCAGG AGGAGCCCTT CCACATTCAT ATCCTGATAA CCACCTTGGT 
CAGCTTGATG TCAATGAATT GTTTGCCAAA CTGTTGTCAA CAGGAATCCT CAAACTGTCA 
AAAACAGATT CCACTTCAGC ACAAGCGAAT GAAACATCTG CACAGCCAGC TGCTGAAGAG 
GAGGATGATG ACCAGGGTGA TCATGATGTC CCAGACCTCA CAAACTTTAA AGTTGAAGAA 
CTCAGACAGC GTTATGATAG TGTTATAAAT CGACTATACA CTGGAATTCA GTGCTACTCG 
TGTGGAATGA GATTTACTAC TTCACAGACA GACGTTTATG CAGACCATTT AGATTGGCAC 
TATCGCCAGA ACCGTTCGGA AAAAGATGTT AGCAGAAAAA TTACACACAG AAGGTGGTAC 
TACAGCGTAA CGGATTGGAT TGAGTTTGAA GAAATAGCTG ACTTAGAGGA GCGTGCAAAA 
AGCCAATTTT TTGAAAAAGC TCATGAAGAA GTTGTACTGA AGACACAGGA AGCTGCAAAA 
GAGAAGGAGT TTCAGAGTGT CCCTGCTGGA CCAGCTGGAG CAGTTGAGAG GTGTGAAATC 
TGCCGGGAGC AATTTGAGCA GTACTGGGAT GAGGAAGAGG AGGAGTGGCA TCTAAAGAAT 
GCTATCAGAA TGCATGAAAA GATTTACCAT CCATCCTGTT ATGAGGATTA CCAAAATACA 
TCATCATTTG ATTGCACACC CTCTCCCAGC AAGACCCCTG TAGAAAATCC CCTAAATATT 
ATGTTGAACA TTGTTAAACA GGAAACAGAT GAGTCCTGTG ACTCACCTCA AGTGAAAGAG 
GAACCTGATG ACACCCCTGC TGCCTGTGCA GAAGAGAGCA CACCTGCATC TATAGATGTT 
AAAACAGAAC CTGATGAGTC TGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( XM_025146901.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002669.1 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X5, mRNA.

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CloneID	OGa143228
Clone ID Related Accession (Same CDS sequence)	XM_025146901.1
Accession Version	XM_025146901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC TGAAAGAAAG TTCTCAGAGA ATGGTGGATG AAAATACTAG GAAAAGTTTA 
TTTAAGTTAC GCTCCACATG GGACGATATT TTTCCTTTGA AGAAACTATA TGCACTTGAC 
GTCAGAGTCA ACTCATTAGA TCCTGCTTGG CCAATTAAAC CTCTGCCCCC AAATGTGAAT 
ACTTCTAGCA TCCATGTGAA TCCTAAGTTT TTAAATAAAT CGCCAGAGGA ATCTACTGCA 
TCTACCTCTG CTGTCACTTC TGGTGCCTCT ACTCCTCCAG CTGTTCCTGA AATACAAAAG 
AATTTGACAC AGGAGCAACT GATAAGGCAG CAATTGCTTG CGAAGCAAAA ACAGTTGTTA 
GAACTTCAGC AAAAAAAGTT AGAGTTGGAG TTGGAACAGA CTAAAGCACA GTTGGCTGTT 
TCTCTAAGTG TTCAGCAAGG ATCATCTTCT ATACCTTCAG TTCCAGCACC TTCCAAGCCA 
CATGTGTCCC AGACACCTCA CATGCCAGTT AAAACACCTA CTCAGGCTTC ACTGCAATCT 
GAAAAGATCA AACCATCCCC AAGTCCTCCA CTTCACGATG TCAAAATAGT AAACAGGGAT 
CCTCGACTTA ATAGAATGTC GCAACATTCT TCTCATGCTA AAGATCAGTC TCATAGGAAA 
GAATTTCCAC CAAACACAAC TGGTCAGTCT GATACCAAGG CAAGCAAAAC ATCCCAGTCT 
GAAAAACAGA GCTCAACAAA ACAAGAGAAA GTGAAAGCTG GTGAAAAAAC ACAGAAGAAA 
GAACCCGATC AATTGGAAAC AAAGTCGAAA TCACCATCTC CTTTGAAAAA CAAGCTTCCG 
AATACTAAAG ATACTAAAAC CCAAGAATCT GAAAGCACAA AAGTATCTGA AATGAGTAAA 
CGGGATCCAA GACTGAAAAA GCATCTTCAA GAGAAACCAG AGAGCAAAGG GGATGAGGTG 
AAAGAAAAGA GGAGAAATAC AGAGAAAAAA GAAAAAGACG AACATAAGAC AGGTGAACAT 
AGGCCAGTAG GCAGCAGAAA TAAAACAGTT AATGGTGTTG TTCAGAAACA AGACGTGACT 
ACAGAAGAAT CGGAAAAACA GGGTGGAAAA CAAGGGAGAT CCAGTAATAG AAAACGGTCA 
CGATCCCGCT CTCCTAAGTC ACGGTCACCA TCTACACACT CTCCAAAACG AAGAGAGAGG 
AGATCACCCA AAAGAAGACT CAGGAGTTTA TCTCCGACTT CTTCAACTCC TAAAATTGGA 
AAGATACGTC AGATAGGTCC TAAGCAGTCT CATACTGAAG AAGCCTCACA AGGGGCAAGA 
GATGAAAGAA ATTCTAATAA GCGAAATGTT AAACAAGAGG TGCGAGATCC AAGGAGAGTG 
AAAAAAAGCC AAGAAGACAG ACCCCAAGAA ATAGCAAGCC AGCATTCTAC AAAAGCTTCT 
CCCGATCCAA AGGAGAATGC AGAGAATTGG CAGGGATCCA AAGCAGGCAA GAGGTGGAAA 
TCCGGTTGGG AGGAAAATAA AAACTCGCAG CAGACTGAAG AACACCAAGC GGCTGGTAAA 
TCCCCTCACC AAAGACACCG AGAAAACTGG CCTGCTGCTA AAGGAATTCT GTCACCTCGT 
GCACCAAAAC AGCAGCACCG GTTAAGCGTG GATGCCAATT TACAAATTCC CAAAGAACTG 
ACATCTGCAA GCAAAAGAGA ATTACTTAAA AAGGCCAATG ACCGCTTAGC ATCTGGTGAA 
ATAACCCAAG ATGAGTTTCT TGTGGTAGCT CATCAGATTC GACAGTTATT CCAGTACCAG 
GAAGGAAAGC ACAGATGTAA TGTGTGGGAT AGCCCTACAG AGGAAAAAGG CTCTTTGAAA 
AGGAAGCCTC TTCTATCTGA TGCAGAATTA ACATACTATG AGCATAAAGC TAAACTGAAA 
AGAACACAAG TTCAGCATTC ATTGTCAAGA CTTGATCTCT TGGATCCTGA TGATATTTTG 
GATTATCGTG TACCAGACGC GTTGCTTTCT GGAATAGACT GTGAGCAAGC GAAAGCAAAG 
CGTGGAGTAC AGTTTGAAAG AAAAGAACCT TTTGGAGAAA GATCAAGACG GCACTCTCCT 
GTAAGTGGAA CAAGCAGACC CTTTGCTGAT AACCTTTCGC CGCTTGAGGG TCGACGAAGG 
CTTGAGGAAC AAAATGCTAC TAAAGGAAGC AGGGGTTCGA AAAACTTCAA TCCTTATGAC 
AACTGGGGAG AATCGGATGA ATTCAGAGAT GCTCTCAGAC AGCAGGGAAA AGGTACAGCA 
GAGTTCCAGA AGATAGATGG TGATGCCGTC TGTAGGTTTG ATAACCGTGA AGAAAGACAG 
CTGCTTGGGA AAGCTGGTGT TCGAGATGAA CCAAGATCAC CATTCAATGA ACGTTTCAAA 
AGAGCTAGAT ACGATGATCC AGATAAAGAA CCATTTCCAG ACAGTCCAGG GTCAAGATTT 
GGAGGCATAG AAGCAAAGCC AAGAATAAGT GCACTAATGG AAGACAGACC TTTATTTGAT 
GGCTCATCTA GACCATCTGC TGCAAGAGTT GGGGCAGATG GACAAGGGAG TCCTTTTATT 
GATAGTCCTA CTGCTGGCTC AAGTTCTAGA ATTGATGGGC CACCTGGACA GGCTGCTCTG 
AGATTTGAGG GGCCTTTGGT GGGGGCAGGT GCTTCTCAGT TTGATGGACC CCTGGCAGGA 
GCAGGGGGAG CTGGAGCCCT GAGATTTGAT GGGCCACCAG GGGCGCTCAG GTTTGAAGGA 
CCACAGGGAC AGGTTGGTGG GGGAGGTCCT CTGAGGTTTG AGGGGCCTAT TGGGCAGATA 
GGTGGGCCAT TGCGGTTTGA GGGGCCTGCA GGCCAGCCTG TGGGTGGTCC TAGATTTGAA 
GGACCTGGAG TTGGTCTCAG GTTTGAGGGA CCCCGTGGTC AGCCTTCAGG TGGTCTCAGG 
TTTGAGGGAC CTCATGGTCA ACCTCTGGGG CCTCGAGGTC AGCCAGGGGG TGGTCTCAGG 
TTTGAGGGAC CGCACGGTCA GCCCTTGGGG CCTCACGGTC AAGCAGGAGG TGGTCTCAGG 
TTTGAGGGTC CGCATTTACA GCCATTGGGG CCCCATGGTC AACCTGGAGG TGGCCTCAGA 
TTTGAGGGGC CACATGGACA GCCTATGGGG CCACATGGAC CGTCAGGAGG TGGGCTCAGA 
TTTGAGGGGC CACATGGACC GCCAGGTGTA GGGCCGAGGT TGATTGATGG ACCAGTGCAC 
CAGGGAGTTG GTGGACTTAG ATTTGATGGT CCTCTGGGGC GGTCTGGTCC GAGATTTGAT 
GGCTGTCATG CTGGCTTTGA TGGTCAACCT GGACAGCTGT CTCTTTTGCA AAGATTTGAT 
GGAATTCATG GACAGCCTGG CCCAAGATTT GAAAGGGCTC CTGGCCAGCC CGCACAACCG 
CGATTTGATG CAGCCATACC TCAAAGATTT GATGGTCCTC ACCAGCCAGC CTCGAGATTT 
GACCTGCCTC TTGGACTTCA CGGTGCACGT TTTGAAAATA TAGCTAGCCA TCCTGCCTCA 
CGATTGGAGA TGTCGCCATA TGGACAAAGT GGTCCGTTTG TTGACCATCC CGGACAGGGT 
TTCAGCGGAC CAGCTCATGG AATGCAGTTC CAAAGACCTG AAATGTTTGA CAGTTCACTA 
GGACCAAATT TCAGTGGGCC AGCTGGCCCA GGAGGACAGA ATTTTTCATT GAGAGCATCT 
GGACATTATT TCGATGACAA ACTTCAGGGT CCTCAATACG GAAACTTCAA TAGTATGTCA 
ATGGGAGGTC ATCAGGTTTC GCACATGTCT GCTCAGCCAG GACCTTATGG CCAAGGTCAA 
CAGTATTTAC CAAATCCTGG AAGCTTTGTT CAGAACCCTG CAGGAGGAGC CCTTCCACAT 
TCATATCCTG ATAACCACCT TGGTCAGCTT GATGTCAATG AATTGTTTGC CAAACTGTTG 
TCAACAGGAA TCCTCAAACT GTCAAAAACA GATTCCACTT CAGCACAAGC GAATGAAACA 
TCTGCACAGC CAGCTGCTGA AGAGGAGGAT GATGACCAGG GTGATCATGA TGTCCCAGAC 
CTCACAAACT TTAAAGTTGA AGAACTCAGA CAGCGTTATG ATAGTGTTAT AAATCGACTA 
TACACTGGAA TTCAGTGCTA CTCGTGTGGA ATGAGATTTA CTACTTCACA GACAGACGTT 
TATGCAGACC ATTTAGATTG GCACTATCGC CAGAACCGTT CGGAAAAAGA TGTTAGCAGA 
AAAATTACAC ACAGAAGGTG GTACTACAGC GTAACGGATT GGATTGAGTT TGAAGAAATA 
GCTGACTTAG AGGAGCGTGC AAAAAGCCAA TTTTTTGAAA AAGCTCATGA AGAAGTTGTA 
CTGAAGACAC AGGAAGCTGC AAAAGAGAAG GAGTTTCAGA GTGTCCCTGC TGGACCAGCT 
GGAGCAGTTG AGAGGTGTGA AATCTGCCGG GAGCAATTTG AGCAGTACTG GGATGAGGAA 
GAGGAGGAGT GGCATCTAAA GAATGCTATC AGAATGCATG AAAAGATTTA CCATCCATCC 
TGTTATGAGG ATTACCAAAA TACATCATCA TTTGATTGCA CACCCTCTCC CAGCAAGACC 
CCTGTAGAAA ATCCCCTAAA TATTATGTTG AACATTGTTA AACAGGAAAC AGATGAGTCC 
TGTGACTCAC CTCAAGTGAA AGAGGAACCT GATGACACCC CTGCTGCCTG TGCAGAAGAG 
AGCACACCTG CATCTATAGA TGTTAAAACA GAACCTGATG AGTCTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002669.1
CDS	2091..6881
Translation	MEKLKESSQRMVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDVTTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAQANETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_025146901.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025146901.1

ORF Insert Sequence:

ATGGAAAAGC TGAAAGAAAG TTCTCAGAGA ATGGTGGATG AAAATACTAG GAAAAGTTTA 
TTTAAGTTAC GCTCCACATG GGACGATATT TTTCCTTTGA AGAAACTATA TGCACTTGAC 
GTCAGAGTCA ACTCATTAGA TCCTGCTTGG CCAATTAAAC CTCTGCCCCC AAATGTGAAT 
ACTTCTAGCA TCCATGTGAA TCCTAAGTTT TTAAATAAAT CGCCAGAGGA ATCTACTGCA 
TCTACCTCTG CTGTCACTTC TGGTGCCTCT ACTCCTCCAG CTGTTCCTGA AATACAAAAG 
AATTTGACAC AGGAGCAACT GATAAGGCAG CAATTGCTTG CGAAGCAAAA ACAGTTGTTA 
GAACTTCAGC AAAAAAAGTT AGAGTTGGAG TTGGAACAGA CTAAAGCACA GTTGGCTGTT 
TCTCTAAGTG TTCAGCAAGG ATCATCTTCT ATACCTTCAG TTCCAGCACC TTCCAAGCCA 
CATGTGTCCC AGACACCTCA CATGCCAGTT AAAACACCTA CTCAGGCTTC ACTGCAATCT 
GAAAAGATCA AACCATCCCC AAGTCCTCCA CTTCACGATG TCAAAATAGT AAACAGGGAT 
CCTCGACTTA ATAGAATGTC GCAACATTCT TCTCATGCTA AAGATCAGTC TCATAGGAAA 
GAATTTCCAC CAAACACAAC TGGTCAGTCT GATACCAAGG CAAGCAAAAC ATCCCAGTCT 
GAAAAACAGA GCTCAACAAA ACAAGAGAAA GTGAAAGCTG GTGAAAAAAC ACAGAAGAAA 
GAACCCGATC AATTGGAAAC AAAGTCGAAA TCACCATCTC CTTTGAAAAA CAAGCTTCCG 
AATACTAAAG ATACTAAAAC CCAAGAATCT GAAAGCACAA AAGTATCTGA AATGAGTAAA 
CGGGATCCAA GACTGAAAAA GCATCTTCAA GAGAAACCAG AGAGCAAAGG GGATGAGGTG 
AAAGAAAAGA GGAGAAATAC AGAGAAAAAA GAAAAAGACG AACATAAGAC AGGTGAACAT 
AGGCCAGTAG GCAGCAGAAA TAAAACAGTT AATGGTGTTG TTCAGAAACA AGACGTGACT 
ACAGAAGAAT CGGAAAAACA GGGTGGAAAA CAAGGGAGAT CCAGTAATAG AAAACGGTCA 
CGATCCCGCT CTCCTAAGTC ACGGTCACCA TCTACACACT CTCCAAAACG AAGAGAGAGG 
AGATCACCCA AAAGAAGACT CAGGAGTTTA TCTCCGACTT CTTCAACTCC TAAAATTGGA 
AAGATACGTC AGATAGGTCC TAAGCAGTCT CATACTGAAG AAGCCTCACA AGGGGCAAGA 
GATGAAAGAA ATTCTAATAA GCGAAATGTT AAACAAGAGG TGCGAGATCC AAGGAGAGTG 
AAAAAAAGCC AAGAAGACAG ACCCCAAGAA ATAGCAAGCC AGCATTCTAC AAAAGCTTCT 
CCCGATCCAA AGGAGAATGC AGAGAATTGG CAGGGATCCA AAGCAGGCAA GAGGTGGAAA 
TCCGGTTGGG AGGAAAATAA AAACTCGCAG CAGACTGAAG AACACCAAGC GGCTGGTAAA 
TCCCCTCACC AAAGACACCG AGAAAACTGG CCTGCTGCTA AAGGAATTCT GTCACCTCGT 
GCACCAAAAC AGCAGCACCG GTTAAGCGTG GATGCCAATT TACAAATTCC CAAAGAACTG 
ACATCTGCAA GCAAAAGAGA ATTACTTAAA AAGGCCAATG ACCGCTTAGC ATCTGGTGAA 
ATAACCCAAG ATGAGTTTCT TGTGGTAGCT CATCAGATTC GACAGTTATT CCAGTACCAG 
GAAGGAAAGC ACAGATGTAA TGTGTGGGAT AGCCCTACAG AGGAAAAAGG CTCTTTGAAA 
AGGAAGCCTC TTCTATCTGA TGCAGAATTA ACATACTATG AGCATAAAGC TAAACTGAAA 
AGAACACAAG TTCAGCATTC ATTGTCAAGA CTTGATCTCT TGGATCCTGA TGATATTTTG 
GATTATCGTG TACCAGACGC GTTGCTTTCT GGAATAGACT GTGAGCAAGC GAAAGCAAAG 
CGTGGAGTAC AGTTTGAAAG AAAAGAACCT TTTGGAGAAA GATCAAGACG GCACTCTCCT 
GTAAGTGGAA CAAGCAGACC CTTTGCTGAT AACCTTTCGC CGCTTGAGGG TCGACGAAGG 
CTTGAGGAAC AAAATGCTAC TAAAGGAAGC AGGGGTTCGA AAAACTTCAA TCCTTATGAC 
AACTGGGGAG AATCGGATGA ATTCAGAGAT GCTCTCAGAC AGCAGGGAAA AGGTACAGCA 
GAGTTCCAGA AGATAGATGG TGATGCCGTC TGTAGGTTTG ATAACCGTGA AGAAAGACAG 
CTGCTTGGGA AAGCTGGTGT TCGAGATGAA CCAAGATCAC CATTCAATGA ACGTTTCAAA 
AGAGCTAGAT ACGATGATCC AGATAAAGAA CCATTTCCAG ACAGTCCAGG GTCAAGATTT 
GGAGGCATAG AAGCAAAGCC AAGAATAAGT GCACTAATGG AAGACAGACC TTTATTTGAT 
GGCTCATCTA GACCATCTGC TGCAAGAGTT GGGGCAGATG GACAAGGGAG TCCTTTTATT 
GATAGTCCTA CTGCTGGCTC AAGTTCTAGA ATTGATGGGC CACCTGGACA GGCTGCTCTG 
AGATTTGAGG GGCCTTTGGT GGGGGCAGGT GCTTCTCAGT TTGATGGACC CCTGGCAGGA 
GCAGGGGGAG CTGGAGCCCT GAGATTTGAT GGGCCACCAG GGGCGCTCAG GTTTGAAGGA 
CCACAGGGAC AGGTTGGTGG GGGAGGTCCT CTGAGGTTTG AGGGGCCTAT TGGGCAGATA 
GGTGGGCCAT TGCGGTTTGA GGGGCCTGCA GGCCAGCCTG TGGGTGGTCC TAGATTTGAA 
GGACCTGGAG TTGGTCTCAG GTTTGAGGGA CCCCGTGGTC AGCCTTCAGG TGGTCTCAGG 
TTTGAGGGAC CTCATGGTCA ACCTCTGGGG CCTCGAGGTC AGCCAGGGGG TGGTCTCAGG 
TTTGAGGGAC CGCACGGTCA GCCCTTGGGG CCTCACGGTC AAGCAGGAGG TGGTCTCAGG 
TTTGAGGGTC CGCATTTACA GCCATTGGGG CCCCATGGTC AACCTGGAGG TGGCCTCAGA 
TTTGAGGGGC CACATGGACA GCCTATGGGG CCACATGGAC CGTCAGGAGG TGGGCTCAGA 
TTTGAGGGGC CACATGGACC GCCAGGTGTA GGGCCGAGGT TGATTGATGG ACCAGTGCAC 
CAGGGAGTTG GTGGACTTAG ATTTGATGGT CCTCTGGGGC GGTCTGGTCC GAGATTTGAT 
GGCTGTCATG CTGGCTTTGA TGGTCAACCT GGACAGCTGT CTCTTTTGCA AAGATTTGAT 
GGAATTCATG GACAGCCTGG CCCAAGATTT GAAAGGGCTC CTGGCCAGCC CGCACAACCG 
CGATTTGATG CAGCCATACC TCAAAGATTT GATGGTCCTC ACCAGCCAGC CTCGAGATTT 
GACCTGCCTC TTGGACTTCA CGGTGCACGT TTTGAAAATA TAGCTAGCCA TCCTGCCTCA 
CGATTGGAGA TGTCGCCATA TGGACAAAGT GGTCCGTTTG TTGACCATCC CGGACAGGGT 
TTCAGCGGAC CAGCTCATGG AATGCAGTTC CAAAGACCTG AAATGTTTGA CAGTTCACTA 
GGACCAAATT TCAGTGGGCC AGCTGGCCCA GGAGGACAGA ATTTTTCATT GAGAGCATCT 
GGACATTATT TCGATGACAA ACTTCAGGGT CCTCAATACG GAAACTTCAA TAGTATGTCA 
ATGGGAGGTC ATCAGGTTTC GCACATGTCT GCTCAGCCAG GACCTTATGG CCAAGGTCAA 
CAGTATTTAC CAAATCCTGG AAGCTTTGTT CAGAACCCTG CAGGAGGAGC CCTTCCACAT 
TCATATCCTG ATAACCACCT TGGTCAGCTT GATGTCAATG AATTGTTTGC CAAACTGTTG 
TCAACAGGAA TCCTCAAACT GTCAAAAACA GATTCCACTT CAGCACAAGC GAATGAAACA 
TCTGCACAGC CAGCTGCTGA AGAGGAGGAT GATGACCAGG GTGATCATGA TGTCCCAGAC 
CTCACAAACT TTAAAGTTGA AGAACTCAGA CAGCGTTATG ATAGTGTTAT AAATCGACTA 
TACACTGGAA TTCAGTGCTA CTCGTGTGGA ATGAGATTTA CTACTTCACA GACAGACGTT 
TATGCAGACC ATTTAGATTG GCACTATCGC CAGAACCGTT CGGAAAAAGA TGTTAGCAGA 
AAAATTACAC ACAGAAGGTG GTACTACAGC GTAACGGATT GGATTGAGTT TGAAGAAATA 
GCTGACTTAG AGGAGCGTGC AAAAAGCCAA TTTTTTGAAA AAGCTCATGA AGAAGTTGTA 
CTGAAGACAC AGGAAGCTGC AAAAGAGAAG GAGTTTCAGA GTGTCCCTGC TGGACCAGCT 
GGAGCAGTTG AGAGGTGTGA AATCTGCCGG GAGCAATTTG AGCAGTACTG GGATGAGGAA 
GAGGAGGAGT GGCATCTAAA GAATGCTATC AGAATGCATG AAAAGATTTA CCATCCATCC 
TGTTATGAGG ATTACCAAAA TACATCATCA TTTGATTGCA CACCCTCTCC CAGCAAGACC 
CCTGTAGAAA ATCCCCTAAA TATTATGTTG AACATTGTTA AACAGGAAAC AGATGAGTCC 
TGTGACTCAC CTCAAGTGAA AGAGGAACCT GATGACACCC CTGCTGCCTG TGCAGAAGAG 
AGCACACCTG CATCTATAGA TGTTAAAACA GAACCTGATG AGTCTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( XM_004938891.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004938948.2 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X1, mRNA.

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CloneID	OGa31099
Clone ID Related Accession (Same CDS sequence)	XM_004938891.2
Accession Version	XM_004938891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455761.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004938891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGCGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC ACCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACAA GCGAATGAAA CATCTGCACA GCCAGCTGCT 
GAAGAGGAGG ATGATGACCA GGGTGATCAT GATGTCCCAG ACCTCACAAA CTTTAAAGTT 
GAAGAACTCA GACAGCGTTA TGATAGTGTT ATAAATCGAC TATACACTGG AATTCAGTGC 
TACTCGTGTG GAATGAGATT TACTACTTCA CAGACAGACG TTTATGCAGA CCATTTAGAT 
TGGCACTATC GCCAGAACCG TTCGGAAAAA GATGTTAGCA GAAAAATTAC ACACAGAAGG 
TGGTACTACA GCGTAACGGA TTGGATTGAG TTTGAAGAAA TAGCCGACTT AGAGGAGCGT 
GCAAAAAGCC AATTTTTTGA AAAAGCTCAT GAAGAAGTTG TACTGAAGAC ACAGGAAGCT 
GCAAAAGAGA AGGAGTTTCA GAGTGTCCCT GCTGGACCAG CTGGAGCAGT TGAGAGGTGT 
GAAATCTGCC GGGAGCAATT TGAGCAGTAC TGGGATGAGG AAGAGGAGGA GTGGCATCTA 
AAGAATGCTA TCAGAATGCA TGAAAAGATT TACCATCCAT CCTGTTATGA GGATTACCAA 
AATACATCAT CATTTGATTG CACACCCTCT CCCAGCAAGA CCCCTGTAGA AAATCCCCTA 
AATATTATGT TGAACATTGT TAAACAGGAA ACAGATGAGT CCTGTGACTC ACCTCAAGTG 
AAAGAGGAAC CTGATGACAC CCCTGCTGCC TGTGCAGAAG AGAGCACACC TGCATCTATA 
GATGTTAAAA CAGAACCTGA TGAGTCTGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004938948.2
CDS	604..5676
Translation	MSAPESSAGSSEAREDACRDYQSSLEDLTFNSKPHINMLTILAEENVPFAKDIVSLIEAQIAKAPASEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDATTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAQANETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_004938891.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004938891.2

ORF Insert Sequence:

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGCGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC ACCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACAA GCGAATGAAA CATCTGCACA GCCAGCTGCT 
GAAGAGGAGG ATGATGACCA GGGTGATCAT GATGTCCCAG ACCTCACAAA CTTTAAAGTT 
GAAGAACTCA GACAGCGTTA TGATAGTGTT ATAAATCGAC TATACACTGG AATTCAGTGC 
TACTCGTGTG GAATGAGATT TACTACTTCA CAGACAGACG TTTATGCAGA CCATTTAGAT 
TGGCACTATC GCCAGAACCG TTCGGAAAAA GATGTTAGCA GAAAAATTAC ACACAGAAGG 
TGGTACTACA GCGTAACGGA TTGGATTGAG TTTGAAGAAA TAGCCGACTT AGAGGAGCGT 
GCAAAAAGCC AATTTTTTGA AAAAGCTCAT GAAGAAGTTG TACTGAAGAC ACAGGAAGCT 
GCAAAAGAGA AGGAGTTTCA GAGTGTCCCT GCTGGACCAG CTGGAGCAGT TGAGAGGTGT 
GAAATCTGCC GGGAGCAATT TGAGCAGTAC TGGGATGAGG AAGAGGAGGA GTGGCATCTA 
AAGAATGCTA TCAGAATGCA TGAAAAGATT TACCATCCAT CCTGTTATGA GGATTACCAA 
AATACATCAT CATTTGATTG CACACCCTCT CCCAGCAAGA CCCCTGTAGA AAATCCCCTA 
AATATTATGT TGAACATTGT TAAACAGGAA ACAGATGAGT CCTGTGACTC ACCTCAAGTG 
AAAGAGGAAC CTGATGACAC CCCTGCTGCC TGTGCAGAAG AGAGCACACC TGCATCTATA 
GATGTTAAAA CAGAACCTGA TGAGTCTGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( XM_004938892.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004938949.2 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X2, mRNA.

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CloneID	OGa31100
Clone ID Related Accession (Same CDS sequence)	XM_004938892.2
Accession Version	XM_004938892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455761.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004938892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGCGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC ACCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACCG AATGAAACAT CTGCACAGCC AGCTGCTGAA 
GAGGAGGATG ATGACCAGGG TGATCATGAT GTCCCAGACC TCACAAACTT TAAAGTTGAA 
GAACTCAGAC AGCGTTATGA TAGTGTTATA AATCGACTAT ACACTGGAAT TCAGTGCTAC 
TCGTGTGGAA TGAGATTTAC TACTTCACAG ACAGACGTTT ATGCAGACCA TTTAGATTGG 
CACTATCGCC AGAACCGTTC GGAAAAAGAT GTTAGCAGAA AAATTACACA CAGAAGGTGG 
TACTACAGCG TAACGGATTG GATTGAGTTT GAAGAAATAG CCGACTTAGA GGAGCGTGCA 
AAAAGCCAAT TTTTTGAAAA AGCTCATGAA GAAGTTGTAC TGAAGACACA GGAAGCTGCA 
AAAGAGAAGG AGTTTCAGAG TGTCCCTGCT GGACCAGCTG GAGCAGTTGA GAGGTGTGAA 
ATCTGCCGGG AGCAATTTGA GCAGTACTGG GATGAGGAAG AGGAGGAGTG GCATCTAAAG 
AATGCTATCA GAATGCATGA AAAGATTTAC CATCCATCCT GTTATGAGGA TTACCAAAAT 
ACATCATCAT TTGATTGCAC ACCCTCTCCC AGCAAGACCC CTGTAGAAAA TCCCCTAAAT 
ATTATGTTGA ACATTGTTAA ACAGGAAACA GATGAGTCCT GTGACTCACC TCAAGTGAAA 
GAGGAACCTG ATGACACCCC TGCTGCCTGT GCAGAAGAGA GCACACCTGC ATCTATAGAT 
GTTAAAACAG AACCTGATGA GTCTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004938949.2
CDS	605..5674
Translation	MSAPESSAGSSEAREDACRDYQSSLEDLTFNSKPHINMLTILAEENVPFAKDIVSLIEAQIAKAPASEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDATTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAPNETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_004938892.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004938892.2

ORF Insert Sequence:

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG CTCCTGCGTC AGAGAAGCTC CCTGTTATGT ACCTTATGGA TTCCATTGTC 
AAGAATGTTG GAAGAGAGTA TCTTACTGCA TTTACTAAAA ACCTAGTTGC AACATTTATT 
TGTGTATTTG AAAAGGTGGA TGAAAATACT AGGAAAAGTT TATTTAAGTT ACGCTCCACA 
TGGGACGATA TTTTTCCTTT GAAGAAACTA TATGCACTTG ACGTCAGAGT CAACTCATTA 
GATCCTGCTT GGCCAATTAA ACCTCTGCCC CCAAATGTGA ATACTTCTAG CATCCATGTG 
AATCCTAAGT TTTTAAATAA ATCGCCAGAG GAATCTACTG CATCTACCTC TGCTGTCACT 
TCTGGTGCCT CTACTCCTCC AGCTGTTCCT GAAATACAAA AGAATTTGAC ACAGGAGCAA 
CTGATAAGGC AGCAATTGCT TGCGAAGCAA AAACAGTTGT TAGAACTTCA GCAAAAAAAG 
TTAGAGTTGG AGTTGGAACA GACTAAAGCA CAGTTGGCTG TTTCTCTAAG TGTTCAGCAA 
GGATCATCTT CTATACCTTC AGTTCCAGCA CCTTCCAAGC CACATGTGTC CCAGACACCT 
CACATGCCAG TTAAAACACC TACTCAGGCT TCACTGCAAT CTGAAAAGAT CAAACCATCC 
CCAAGTCCTC CACTTCACGA TGTCAAAATA GTAAACAGGG ATCCTCGACT TAATAGAATG 
TCGCAACATT CTTCTCATGC TAAAGATCAG TCTCATAGGA AAGAATTTCC ACCAAACACA 
ACTGGTCAGT CTGATACCAA GGCAAGCAAA ACATCCCAGT CTGAAAAACA GAGCTCAACA 
AAACAAGAGA AAGTGAAAGC TGGTGAAAAA ACACAGAAGA AAGAACCCGA TCAATTGGAA 
ACAAAGTCGA AATCACCATC TCCTTTGAAA AACAAGCTTC CGAATACTAA AGATACTAAA 
ACCCAAGAAT CTGAAAGCAC AAAAGTATCT GAAATGAGTA AACGGGATCC AAGACTGAAA 
AAGCATCTTC AAGAGAAACC AGAGAGCAAA GGGGATGAGG TGAAAGAAAA GAGGAGAAAT 
ACAGAGAAAA AAGAAAAAGA CGAACATAAG ACAGGTGAAC ATAGGCCAGT AGGCAGCAGA 
AATAAAACAG TTAATGGTGT TGTTCAGAAA CAAGACGCGA CTACAGAAGA ATCGGAAAAA 
CAGGGTGGAA AACAAGGGAG ATCCAGTAAT AGAAAACGGT CACGATCCCG CTCTCCTAAG 
TCACGGTCAC CATCTACACA CTCTCCAAAA CGAAGAGAGA GGAGATCACC CAAAAGAAGA 
CTCAGGAGTT TATCTCCGAC TTCTTCAACT CCTAAAATTG GAAAGATACG TCAGATAGGT 
CCTAAGCAGT CTCATACTGA AGAAGCCTCA CAAGGGGCAA GAGATGAAAG AAATTCTAAT 
AAGCGAAATG TTAAACAAGA GGTGCGAGAT CCAAGGAGAG TGAAAAAAAG CCAAGAAGAC 
AGACCCCAAG AAATAGCAAG CCAGCATTCT ACAAAAGCTT CTCCCGATCC AAAGGAGAAT 
GCAGAGAATT GGCAGGGATC CAAAGCAGGC AAGAGGTGGA AATCCGGTTG GGAGGAAAAT 
AAAAACTCGC AGCAGACTGA AGAACACCAA GCGGCTGGTA AATCCCCTCA CCAAAGACAC 
CGAGAAAACT GGCCTGCTGC TAAAGGAATT CTGTCACCTC GTGCACCAAA ACAGCAGCAC 
CGGTTAAGCG TGGATGCCAA TTTACAAATT CCCAAAGAAC TGACATCTGC AAGCAAAAGA 
GAATTACTTA AAAAGGCCAA TGACCGCTTA GCATCTGGTG AAATAACCCA AGATGAGTTT 
CTTGTGGTAG CTCATCAGAT TCGACAGTTA TTCCAGTACC AGGAAGGAAA GCACAGATGT 
AATGTGTGGG ATAGCCCTAC AGAGGAAAAA GGCTCTTTGA AAAGGAAGCC TCTTCTATCT 
GATGCAGAAT TAACATACTA TGAGCATAAA GCTAAACTGA AAAGAACACA AGTTCAGCAT 
TCATTGTCAA GACTTGATCT CTTGGATCCT GATGATATTT TGGATTATCG TGTACCAGAC 
GCGTTGCTTT CTGGAATAGA CTGTGAGCAA GCGAAAGCAA AGCGTGGAGT ACAGTTTGAA 
AGAAAAGAAC CTTTTGGAGA AAGATCAAGA CGGCACTCTC CTGTAAGTGG AACAAGCAGA 
CCCTTTGCTG ATAACCTTTC ACCGCTTGAG GGTCGACGAA GGCTTGAGGA ACAAAATGCT 
ACTAAAGGAA GCAGGGGTTC GAAAAACTTC AATCCTTATG ACAACTGGGG AGAATCGGAT 
GAATTCAGAG ATGCTCTCAG ACAGCAGGGA AAAGGTACAG CAGAGTTCCA GAAGATAGAT 
GGTGATGCCG TCTGTAGGTT TGATAACCGT GAAGAAAGAC AGCTGCTTGG GAAAGCTGGT 
GTTCGAGATG AACCAAGATC ACCATTCAAT GAACGTTTCA AAAGAGCTAG ATACGATGAT 
CCAGATAAAG AACCATTTCC AGACAGTCCA GGGTCAAGAT TTGGAGGCAT AGAAGCAAAG 
CCAAGAATAA GTGCACTAAT GGAAGACAGA CCTTTATTTG ATGGCTCATC TAGACCATCT 
GCTGCAAGAG TTGGGGCAGA TGGACAAGGG AGTCCTTTTA TTGATAGTCC TACTGCTGGC 
TCAAGTTCTA GAATTGATGG GCCACCTGGA CAGGCTGCTC TGAGATTTGA GGGGCCTTTG 
GTGGGGGCAG GTGCTTCTCA GTTTGATGGA CCCCTGGCAG GAGCAGGGGG AGCTGGAGCC 
CTGAGATTTG ATGGGCCACC AGGGGCGCTC AGGTTTGAAG GACCACAGGG ACAGGTTGGT 
GGGGGAGGTC CTCTGAGGTT TGAGGGGCCT ATTGGGCAGA TAGGTGGGCC ATTGCGGTTT 
GAGGGGCCTG CAGGCCAGCC TGTGGGTGGT CCTAGATTTG AAGGACCTGG AGTTGGTCTC 
AGGTTTGAGG GACCCCGTGG TCAGCCTTCA GGTGGTCTCA GGTTTGAGGG ACCTCATGGT 
CAACCTCTGG GGCCTCGAGG TCAGCCAGGG GGTGGTCTCA GGTTTGAGGG ACCGCACGGT 
CAGCCCTTGG GGCCTCACGG TCAAGCAGGA GGTGGTCTCA GGTTTGAGGG TCCGCATTTA 
CAGCCATTGG GGCCCCATGG TCAACCTGGA GGTGGCCTCA GATTTGAGGG GCCACATGGA 
CAGCCTATGG GGCCACATGG ACCGTCAGGA GGTGGGCTCA GATTTGAGGG GCCACATGGA 
CCGCCAGGTG TAGGGCCGAG GTTGATTGAT GGACCAGTGC ACCAGGGAGT TGGTGGACTT 
AGATTTGATG GTCCTCTGGG GCGGTCTGGT CCGAGATTTG ATGGCTGTCA TGCTGGCTTT 
GATGGTCAAC CTGGACAGCT GTCTCTTTTG CAAAGATTTG ATGGAATTCA TGGACAGCCT 
GGCCCAAGAT TTGAAAGGGC TCCTGGCCAG CCCGCACAAC CGCGATTTGA TGCAGCCATA 
CCTCAAAGAT TTGATGGTCC TCACCAGCCA GCCTCGAGAT TTGACCTGCC TCTTGGACTT 
CACGGTGCAC GTTTTGAAAA TATAGCTAGC CATCCTGCCT CACGATTGGA GATGTCGCCA 
TATGGACAAA GTGGTCCGTT TGTTGACCAT CCCGGACAGG GTTTCAGCGG ACCAGCTCAT 
GGAATGCAGT TCCAAAGACC TGAAATGTTT GACAGTTCAC TAGGACCAAA TTTCAGTGGG 
CCAGCTGGCC CAGGAGGACA GAATTTTTCA TTGAGAGCAT CTGGACATTA TTTCGATGAC 
AAACTTCAGG GTCCTCAATA CGGAAACTTC AATAGTATGT CAATGGGAGG TCATCAGGTT 
TCGCACATGT CTGCTCAGCC AGGACCTTAT GGCCAAGGTC AACAGTATTT ACCAAATCCT 
GGAAGCTTTG TTCAGAACCC TGCAGGAGGA GCCCTTCCAC ATTCATATCC TGATAACCAC 
CTTGGTCAGC TTGATGTCAA TGAATTGTTT GCCAAACTGT TGTCAACAGG AATCCTCAAA 
CTGTCAAAAA CAGATTCCAC TTCAGCACCG AATGAAACAT CTGCACAGCC AGCTGCTGAA 
GAGGAGGATG ATGACCAGGG TGATCATGAT GTCCCAGACC TCACAAACTT TAAAGTTGAA 
GAACTCAGAC AGCGTTATGA TAGTGTTATA AATCGACTAT ACACTGGAAT TCAGTGCTAC 
TCGTGTGGAA TGAGATTTAC TACTTCACAG ACAGACGTTT ATGCAGACCA TTTAGATTGG 
CACTATCGCC AGAACCGTTC GGAAAAAGAT GTTAGCAGAA AAATTACACA CAGAAGGTGG 
TACTACAGCG TAACGGATTG GATTGAGTTT GAAGAAATAG CCGACTTAGA GGAGCGTGCA 
AAAAGCCAAT TTTTTGAAAA AGCTCATGAA GAAGTTGTAC TGAAGACACA GGAAGCTGCA 
AAAGAGAAGG AGTTTCAGAG TGTCCCTGCT GGACCAGCTG GAGCAGTTGA GAGGTGTGAA 
ATCTGCCGGG AGCAATTTGA GCAGTACTGG GATGAGGAAG AGGAGGAGTG GCATCTAAAG 
AATGCTATCA GAATGCATGA AAAGATTTAC CATCCATCCT GTTATGAGGA TTACCAAAAT 
ACATCATCAT TTGATTGCAC ACCCTCTCCC AGCAAGACCC CTGTAGAAAA TCCCCTAAAT 
ATTATGTTGA ACATTGTTAA ACAGGAAACA GATGAGTCCT GTGACTCACC TCAAGTGAAA 
GAGGAACCTG ATGACACCCC TGCTGCCTGT GCAGAAGAGA GCACACCTGC ATCTATAGAT 
GTTAAAACAG AACCTGATGA GTCTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( XM_004938893.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004938950.2 Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X3, mRNA.

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CloneID	OGa31101
Clone ID Related Accession (Same CDS sequence)	XM_004938893.2
Accession Version	XM_004938893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455761.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004938893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG TGGATGAAAA TACTAGGAAA AGTTTATTTA AGTTACGCTC CACATGGGAC 
GATATTTTTC CTTTGAAGAA ACTATATGCA CTTGACGTCA GAGTCAACTC ATTAGATCCT 
GCTTGGCCAA TTAAACCTCT GCCCCCAAAT GTGAATACTT CTAGCATCCA TGTGAATCCT 
AAGTTTTTAA ATAAATCGCC AGAGGAATCT ACTGCATCTA CCTCTGCTGT CACTTCTGGT 
GCCTCTACTC CTCCAGCTGT TCCTGAAATA CAAAAGAATT TGACACAGGA GCAACTGATA 
AGGCAGCAAT TGCTTGCGAA GCAAAAACAG TTGTTAGAAC TTCAGCAAAA AAAGTTAGAG 
TTGGAGTTGG AACAGACTAA AGCACAGTTG GCTGTTTCTC TAAGTGTTCA GCAAGGATCA 
TCTTCTATAC CTTCAGTTCC AGCACCTTCC AAGCCACATG TGTCCCAGAC ACCTCACATG 
CCAGTTAAAA CACCTACTCA GGCTTCACTG CAATCTGAAA AGATCAAACC ATCCCCAAGT 
CCTCCACTTC ACGATGTCAA AATAGTAAAC AGGGATCCTC GACTTAATAG AATGTCGCAA 
CATTCTTCTC ATGCTAAAGA TCAGTCTCAT AGGAAAGAAT TTCCACCAAA CACAACTGGT 
CAGTCTGATA CCAAGGCAAG CAAAACATCC CAGTCTGAAA AACAGAGCTC AACAAAACAA 
GAGAAAGTGA AAGCTGGTGA AAAAACACAG AAGAAAGAAC CCGATCAATT GGAAACAAAG 
TCGAAATCAC CATCTCCTTT GAAAAACAAG CTTCCGAATA CTAAAGATAC TAAAACCCAA 
GAATCTGAAA GCACAAAAGT ATCTGAAATG AGTAAACGGG ATCCAAGACT GAAAAAGCAT 
CTTCAAGAGA AACCAGAGAG CAAAGGGGAT GAGGTGAAAG AAAAGAGGAG AAATACAGAG 
AAAAAAGAAA AAGACGAACA TAAGACAGGT GAACATAGGC CAGTAGGCAG CAGAAATAAA 
ACAGTTAATG GTGTTGTTCA GAAACAAGAC GCGACTACAG AAGAATCGGA AAAACAGGGT 
GGAAAACAAG GGAGATCCAG TAATAGAAAA CGGTCACGAT CCCGCTCTCC TAAGTCACGG 
TCACCATCTA CACACTCTCC AAAACGAAGA GAGAGGAGAT CACCCAAAAG AAGACTCAGG 
AGTTTATCTC CGACTTCTTC AACTCCTAAA ATTGGAAAGA TACGTCAGAT AGGTCCTAAG 
CAGTCTCATA CTGAAGAAGC CTCACAAGGG GCAAGAGATG AAAGAAATTC TAATAAGCGA 
AATGTTAAAC AAGAGGTGCG AGATCCAAGG AGAGTGAAAA AAAGCCAAGA AGACAGACCC 
CAAGAAATAG CAAGCCAGCA TTCTACAAAA GCTTCTCCCG ATCCAAAGGA GAATGCAGAG 
AATTGGCAGG GATCCAAAGC AGGCAAGAGG TGGAAATCCG GTTGGGAGGA AAATAAAAAC 
TCGCAGCAGA CTGAAGAACA CCAAGCGGCT GGTAAATCCC CTCACCAAAG ACACCGAGAA 
AACTGGCCTG CTGCTAAAGG AATTCTGTCA CCTCGTGCAC CAAAACAGCA GCACCGGTTA 
AGCGTGGATG CCAATTTACA AATTCCCAAA GAACTGACAT CTGCAAGCAA AAGAGAATTA 
CTTAAAAAGG CCAATGACCG CTTAGCATCT GGTGAAATAA CCCAAGATGA GTTTCTTGTG 
GTAGCTCATC AGATTCGACA GTTATTCCAG TACCAGGAAG GAAAGCACAG ATGTAATGTG 
TGGGATAGCC CTACAGAGGA AAAAGGCTCT TTGAAAAGGA AGCCTCTTCT ATCTGATGCA 
GAATTAACAT ACTATGAGCA TAAAGCTAAA CTGAAAAGAA CACAAGTTCA GCATTCATTG 
TCAAGACTTG ATCTCTTGGA TCCTGATGAT ATTTTGGATT ATCGTGTACC AGACGCGTTG 
CTTTCTGGAA TAGACTGTGA GCAAGCGAAA GCAAAGCGTG GAGTACAGTT TGAAAGAAAA 
GAACCTTTTG GAGAAAGATC AAGACGGCAC TCTCCTGTAA GTGGAACAAG CAGACCCTTT 
GCTGATAACC TTTCACCGCT TGAGGGTCGA CGAAGGCTTG AGGAACAAAA TGCTACTAAA 
GGAAGCAGGG GTTCGAAAAA CTTCAATCCT TATGACAACT GGGGAGAATC GGATGAATTC 
AGAGATGCTC TCAGACAGCA GGGAAAAGGT ACAGCAGAGT TCCAGAAGAT AGATGGTGAT 
GCCGTCTGTA GGTTTGATAA CCGTGAAGAA AGACAGCTGC TTGGGAAAGC TGGTGTTCGA 
GATGAACCAA GATCACCATT CAATGAACGT TTCAAAAGAG CTAGATACGA TGATCCAGAT 
AAAGAACCAT TTCCAGACAG TCCAGGGTCA AGATTTGGAG GCATAGAAGC AAAGCCAAGA 
ATAAGTGCAC TAATGGAAGA CAGACCTTTA TTTGATGGCT CATCTAGACC ATCTGCTGCA 
AGAGTTGGGG CAGATGGACA AGGGAGTCCT TTTATTGATA GTCCTACTGC TGGCTCAAGT 
TCTAGAATTG ATGGGCCACC TGGACAGGCT GCTCTGAGAT TTGAGGGGCC TTTGGTGGGG 
GCAGGTGCTT CTCAGTTTGA TGGACCCCTG GCAGGAGCAG GGGGAGCTGG AGCCCTGAGA 
TTTGATGGGC CACCAGGGGC GCTCAGGTTT GAAGGACCAC AGGGACAGGT TGGTGGGGGA 
GGTCCTCTGA GGTTTGAGGG GCCTATTGGG CAGATAGGTG GGCCATTGCG GTTTGAGGGG 
CCTGCAGGCC AGCCTGTGGG TGGTCCTAGA TTTGAAGGAC CTGGAGTTGG TCTCAGGTTT 
GAGGGACCCC GTGGTCAGCC TTCAGGTGGT CTCAGGTTTG AGGGACCTCA TGGTCAACCT 
CTGGGGCCTC GAGGTCAGCC AGGGGGTGGT CTCAGGTTTG AGGGACCGCA CGGTCAGCCC 
TTGGGGCCTC ACGGTCAAGC AGGAGGTGGT CTCAGGTTTG AGGGTCCGCA TTTACAGCCA 
TTGGGGCCCC ATGGTCAACC TGGAGGTGGC CTCAGATTTG AGGGGCCACA TGGACAGCCT 
ATGGGGCCAC ATGGACCGTC AGGAGGTGGG CTCAGATTTG AGGGGCCACA TGGACCGCCA 
GGTGTAGGGC CGAGGTTGAT TGATGGACCA GTGCACCAGG GAGTTGGTGG ACTTAGATTT 
GATGGTCCTC TGGGGCGGTC TGGTCCGAGA TTTGATGGCT GTCATGCTGG CTTTGATGGT 
CAACCTGGAC AGCTGTCTCT TTTGCAAAGA TTTGATGGAA TTCATGGACA GCCTGGCCCA 
AGATTTGAAA GGGCTCCTGG CCAGCCCGCA CAACCGCGAT TTGATGCAGC CATACCTCAA 
AGATTTGATG GTCCTCACCA GCCAGCCTCG AGATTTGACC TGCCTCTTGG ACTTCACGGT 
GCACGTTTTG AAAATATAGC TAGCCATCCT GCCTCACGAT TGGAGATGTC GCCATATGGA 
CAAAGTGGTC CGTTTGTTGA CCATCCCGGA CAGGGTTTCA GCGGACCAGC TCATGGAATG 
CAGTTCCAAA GACCTGAAAT GTTTGACAGT TCACTAGGAC CAAATTTCAG TGGGCCAGCT 
GGCCCAGGAG GACAGAATTT TTCATTGAGA GCATCTGGAC ATTATTTCGA TGACAAACTT 
CAGGGTCCTC AATACGGAAA CTTCAATAGT ATGTCAATGG GAGGTCATCA GGTTTCGCAC 
ATGTCTGCTC AGCCAGGACC TTATGGCCAA GGTCAACAGT ATTTACCAAA TCCTGGAAGC 
TTTGTTCAGA ACCCTGCAGG AGGAGCCCTT CCACATTCAT ATCCTGATAA CCACCTTGGT 
CAGCTTGATG TCAATGAATT GTTTGCCAAA CTGTTGTCAA CAGGAATCCT CAAACTGTCA 
AAAACAGATT CCACTTCAGC ACAAGCGAAT GAAACATCTG CACAGCCAGC TGCTGAAGAG 
GAGGATGATG ACCAGGGTGA TCATGATGTC CCAGACCTCA CAAACTTTAA AGTTGAAGAA 
CTCAGACAGC GTTATGATAG TGTTATAAAT CGACTATACA CTGGAATTCA GTGCTACTCG 
TGTGGAATGA GATTTACTAC TTCACAGACA GACGTTTATG CAGACCATTT AGATTGGCAC 
TATCGCCAGA ACCGTTCGGA AAAAGATGTT AGCAGAAAAA TTACACACAG AAGGTGGTAC 
TACAGCGTAA CGGATTGGAT TGAGTTTGAA GAAATAGCCG ACTTAGAGGA GCGTGCAAAA 
AGCCAATTTT TTGAAAAAGC TCATGAAGAA GTTGTACTGA AGACACAGGA AGCTGCAAAA 
GAGAAGGAGT TTCAGAGTGT CCCTGCTGGA CCAGCTGGAG CAGTTGAGAG GTGTGAAATC 
TGCCGGGAGC AATTTGAGCA GTACTGGGAT GAGGAAGAGG AGGAGTGGCA TCTAAAGAAT 
GCTATCAGAA TGCATGAAAA GATTTACCAT CCATCCTGTT ATGAGGATTA CCAAAATACA 
TCATCATTTG ATTGCACACC CTCTCCCAGC AAGACCCCTG TAGAAAATCC CCTAAATATT 
ATGTTGAACA TTGTTAAACA GGAAACAGAT GAGTCCTGTG ACTCACCTCA AGTGAAAGAG 
GAACCTGATG ACACCCCTGC TGCCTGTGCA GAAGAGAGCA CACCTGCATC TATAGATGTT 
AAAACAGAAC CTGATGAGTC TGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004938950.2
CDS	603..5549
Translation	MSAPESSAGSSEAREDACRDYQSSLEDLTFNSKPHINMLTILAEENVPFAKDIVSLIEAQIAKVDENTRKSLFKLRSTWDDIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEESTASTSAVTSGASTPPAVPEIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQTKAQLAVSLSVQQGSSSIPSVPAPSKPHVSQTPHMPVKTPTQASLQSEKIKPSPSPPLHDVKIVNRDPRLNRMSQHSSHAKDQSHRKEFPPNTTGQSDTKASKTSQSEKQSSTKQEKVKAGEKTQKKEPDQLETKSKSPSPLKNKLPNTKDTKTQESESTKVSEMSKRDPRLKKHLQEKPESKGDEVKEKRRNTEKKEKDEHKTGEHRPVGSRNKTVNGVVQKQDATTEESEKQGGKQGRSSNRKRSRSRSPKSRSPSTHSPKRRERRSPKRRLRSLSPTSSTPKIGKIRQIGPKQSHTEEASQGARDERNSNKRNVKQEVRDPRRVKKSQEDRPQEIASQHSTKASPDPKENAENWQGSKAGKRWKSGWEENKNSQQTEEHQAAGKSPHQRHRENWPAAKGILSPRAPKQQHRLSVDANLQIPKELTSASKRELLKKANDRLASGEITQDEFLVVAHQIRQLFQYQEGKHRCNVWDSPTEEKGSLKRKPLLSDAELTYYEHKAKLKRTQVQHSLSRLDLLDPDDILDYRVPDALLSGIDCEQAKAKRGVQFERKEPFGERSRRHSPVSGTSRPFADNLSPLEGRRRLEEQNATKGSRGSKNFNPYDNWGESDEFRDALRQQGKGTAEFQKIDGDAVCRFDNREERQLLGKAGVRDEPRSPFNERFKRARYDDPDKEPFPDSPGSRFGGIEAKPRISALMEDRPLFDGSSRPSAARVGADGQGSPFIDSPTAGSSSRIDGPPGQAALRFEGPLVGAGASQFDGPLAGAGGAGALRFDGPPGALRFEGPQGQVGGGGPLRFEGPIGQIGGPLRFEGPAGQPVGGPRFEGPGVGLRFEGPRGQPSGGLRFEGPHGQPLGPRGQPGGGLRFEGPHGQPLGPHGQAGGGLRFEGPHLQPLGPHGQPGGGLRFEGPHGQPMGPHGPSGGGLRFEGPHGPPGVGPRLIDGPVHQGVGGLRFDGPLGRSGPRFDGCHAGFDGQPGQLSLLQRFDGIHGQPGPRFERAPGQPAQPRFDAAIPQRFDGPHQPASRFDLPLGLHGARFENIASHPASRLEMSPYGQSGPFVDHPGQGFSGPAHGMQFQRPEMFDSSLGPNFSGPAGPGGQNFSLRASGHYFDDKLQGPQYGNFNSMSMGGHQVSHMSAQPGPYGQGQQYLPNPGSFVQNPAGGALPHSYPDNHLGQLDVNELFAKLLSTGILKLSKTDSTSAQANETSAQPAAEEEDDDQGDHDVPDLTNFKVEELRQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRSEKDVSRKITHRRWYYSVTDWIEFEEIADLEERAKSQFFEKAHEEVVLKTQEAAKEKEFQSVPAGPAGAVERCEICREQFEQYWDEEEEEWHLKNAIRMHEKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKQETDESCDSPQVKEEPDDTPAACAEESTPASIDVKTEPDESV

Target ORF information:

RefSeq Version	XM_004938893.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004938893.2

ORF Insert Sequence:

ATGTCGGCCC CGGAGAGCAG CGCTGGTAGC AGCGAGGCCC GGGAGGACGC GTGCCGCGAT 
TACCAGTCAT CGCTGGAGGA CCTGACGTTC AACAGCAAGC CGCACATCAA CATGCTGACC 
ATCCTGGCCG AGGAGAACGT GCCCTTCGCC AAGGACATCG TCTCCCTGAT CGAGGCGCAA 
ATCGCCAAGG TGGATGAAAA TACTAGGAAA AGTTTATTTA AGTTACGCTC CACATGGGAC 
GATATTTTTC CTTTGAAGAA ACTATATGCA CTTGACGTCA GAGTCAACTC ATTAGATCCT 
GCTTGGCCAA TTAAACCTCT GCCCCCAAAT GTGAATACTT CTAGCATCCA TGTGAATCCT 
AAGTTTTTAA ATAAATCGCC AGAGGAATCT ACTGCATCTA CCTCTGCTGT CACTTCTGGT 
GCCTCTACTC CTCCAGCTGT TCCTGAAATA CAAAAGAATT TGACACAGGA GCAACTGATA 
AGGCAGCAAT TGCTTGCGAA GCAAAAACAG TTGTTAGAAC TTCAGCAAAA AAAGTTAGAG 
TTGGAGTTGG AACAGACTAA AGCACAGTTG GCTGTTTCTC TAAGTGTTCA GCAAGGATCA 
TCTTCTATAC CTTCAGTTCC AGCACCTTCC AAGCCACATG TGTCCCAGAC ACCTCACATG 
CCAGTTAAAA CACCTACTCA GGCTTCACTG CAATCTGAAA AGATCAAACC ATCCCCAAGT 
CCTCCACTTC ACGATGTCAA AATAGTAAAC AGGGATCCTC GACTTAATAG AATGTCGCAA 
CATTCTTCTC ATGCTAAAGA TCAGTCTCAT AGGAAAGAAT TTCCACCAAA CACAACTGGT 
CAGTCTGATA CCAAGGCAAG CAAAACATCC CAGTCTGAAA AACAGAGCTC AACAAAACAA 
GAGAAAGTGA AAGCTGGTGA AAAAACACAG AAGAAAGAAC CCGATCAATT GGAAACAAAG 
TCGAAATCAC CATCTCCTTT GAAAAACAAG CTTCCGAATA CTAAAGATAC TAAAACCCAA 
GAATCTGAAA GCACAAAAGT ATCTGAAATG AGTAAACGGG ATCCAAGACT GAAAAAGCAT 
CTTCAAGAGA AACCAGAGAG CAAAGGGGAT GAGGTGAAAG AAAAGAGGAG AAATACAGAG 
AAAAAAGAAA AAGACGAACA TAAGACAGGT GAACATAGGC CAGTAGGCAG CAGAAATAAA 
ACAGTTAATG GTGTTGTTCA GAAACAAGAC GCGACTACAG AAGAATCGGA AAAACAGGGT 
GGAAAACAAG GGAGATCCAG TAATAGAAAA CGGTCACGAT CCCGCTCTCC TAAGTCACGG 
TCACCATCTA CACACTCTCC AAAACGAAGA GAGAGGAGAT CACCCAAAAG AAGACTCAGG 
AGTTTATCTC CGACTTCTTC AACTCCTAAA ATTGGAAAGA TACGTCAGAT AGGTCCTAAG 
CAGTCTCATA CTGAAGAAGC CTCACAAGGG GCAAGAGATG AAAGAAATTC TAATAAGCGA 
AATGTTAAAC AAGAGGTGCG AGATCCAAGG AGAGTGAAAA AAAGCCAAGA AGACAGACCC 
CAAGAAATAG CAAGCCAGCA TTCTACAAAA GCTTCTCCCG ATCCAAAGGA GAATGCAGAG 
AATTGGCAGG GATCCAAAGC AGGCAAGAGG TGGAAATCCG GTTGGGAGGA AAATAAAAAC 
TCGCAGCAGA CTGAAGAACA CCAAGCGGCT GGTAAATCCC CTCACCAAAG ACACCGAGAA 
AACTGGCCTG CTGCTAAAGG AATTCTGTCA CCTCGTGCAC CAAAACAGCA GCACCGGTTA 
AGCGTGGATG CCAATTTACA AATTCCCAAA GAACTGACAT CTGCAAGCAA AAGAGAATTA 
CTTAAAAAGG CCAATGACCG CTTAGCATCT GGTGAAATAA CCCAAGATGA GTTTCTTGTG 
GTAGCTCATC AGATTCGACA GTTATTCCAG TACCAGGAAG GAAAGCACAG ATGTAATGTG 
TGGGATAGCC CTACAGAGGA AAAAGGCTCT TTGAAAAGGA AGCCTCTTCT ATCTGATGCA 
GAATTAACAT ACTATGAGCA TAAAGCTAAA CTGAAAAGAA CACAAGTTCA GCATTCATTG 
TCAAGACTTG ATCTCTTGGA TCCTGATGAT ATTTTGGATT ATCGTGTACC AGACGCGTTG 
CTTTCTGGAA TAGACTGTGA GCAAGCGAAA GCAAAGCGTG GAGTACAGTT TGAAAGAAAA 
GAACCTTTTG GAGAAAGATC AAGACGGCAC TCTCCTGTAA GTGGAACAAG CAGACCCTTT 
GCTGATAACC TTTCACCGCT TGAGGGTCGA CGAAGGCTTG AGGAACAAAA TGCTACTAAA 
GGAAGCAGGG GTTCGAAAAA CTTCAATCCT TATGACAACT GGGGAGAATC GGATGAATTC 
AGAGATGCTC TCAGACAGCA GGGAAAAGGT ACAGCAGAGT TCCAGAAGAT AGATGGTGAT 
GCCGTCTGTA GGTTTGATAA CCGTGAAGAA AGACAGCTGC TTGGGAAAGC TGGTGTTCGA 
GATGAACCAA GATCACCATT CAATGAACGT TTCAAAAGAG CTAGATACGA TGATCCAGAT 
AAAGAACCAT TTCCAGACAG TCCAGGGTCA AGATTTGGAG GCATAGAAGC AAAGCCAAGA 
ATAAGTGCAC TAATGGAAGA CAGACCTTTA TTTGATGGCT CATCTAGACC ATCTGCTGCA 
AGAGTTGGGG CAGATGGACA AGGGAGTCCT TTTATTGATA GTCCTACTGC TGGCTCAAGT 
TCTAGAATTG ATGGGCCACC TGGACAGGCT GCTCTGAGAT TTGAGGGGCC TTTGGTGGGG 
GCAGGTGCTT CTCAGTTTGA TGGACCCCTG GCAGGAGCAG GGGGAGCTGG AGCCCTGAGA 
TTTGATGGGC CACCAGGGGC GCTCAGGTTT GAAGGACCAC AGGGACAGGT TGGTGGGGGA 
GGTCCTCTGA GGTTTGAGGG GCCTATTGGG CAGATAGGTG GGCCATTGCG GTTTGAGGGG 
CCTGCAGGCC AGCCTGTGGG TGGTCCTAGA TTTGAAGGAC CTGGAGTTGG TCTCAGGTTT 
GAGGGACCCC GTGGTCAGCC TTCAGGTGGT CTCAGGTTTG AGGGACCTCA TGGTCAACCT 
CTGGGGCCTC GAGGTCAGCC AGGGGGTGGT CTCAGGTTTG AGGGACCGCA CGGTCAGCCC 
TTGGGGCCTC ACGGTCAAGC AGGAGGTGGT CTCAGGTTTG AGGGTCCGCA TTTACAGCCA 
TTGGGGCCCC ATGGTCAACC TGGAGGTGGC CTCAGATTTG AGGGGCCACA TGGACAGCCT 
ATGGGGCCAC ATGGACCGTC AGGAGGTGGG CTCAGATTTG AGGGGCCACA TGGACCGCCA 
GGTGTAGGGC CGAGGTTGAT TGATGGACCA GTGCACCAGG GAGTTGGTGG ACTTAGATTT 
GATGGTCCTC TGGGGCGGTC TGGTCCGAGA TTTGATGGCT GTCATGCTGG CTTTGATGGT 
CAACCTGGAC AGCTGTCTCT TTTGCAAAGA TTTGATGGAA TTCATGGACA GCCTGGCCCA 
AGATTTGAAA GGGCTCCTGG CCAGCCCGCA CAACCGCGAT TTGATGCAGC CATACCTCAA 
AGATTTGATG GTCCTCACCA GCCAGCCTCG AGATTTGACC TGCCTCTTGG ACTTCACGGT 
GCACGTTTTG AAAATATAGC TAGCCATCCT GCCTCACGAT TGGAGATGTC GCCATATGGA 
CAAAGTGGTC CGTTTGTTGA CCATCCCGGA CAGGGTTTCA GCGGACCAGC TCATGGAATG 
CAGTTCCAAA GACCTGAAAT GTTTGACAGT TCACTAGGAC CAAATTTCAG TGGGCCAGCT 
GGCCCAGGAG GACAGAATTT TTCATTGAGA GCATCTGGAC ATTATTTCGA TGACAAACTT 
CAGGGTCCTC AATACGGAAA CTTCAATAGT ATGTCAATGG GAGGTCATCA GGTTTCGCAC 
ATGTCTGCTC AGCCAGGACC TTATGGCCAA GGTCAACAGT ATTTACCAAA TCCTGGAAGC 
TTTGTTCAGA ACCCTGCAGG AGGAGCCCTT CCACATTCAT ATCCTGATAA CCACCTTGGT 
CAGCTTGATG TCAATGAATT GTTTGCCAAA CTGTTGTCAA CAGGAATCCT CAAACTGTCA 
AAAACAGATT CCACTTCAGC ACAAGCGAAT GAAACATCTG CACAGCCAGC TGCTGAAGAG 
GAGGATGATG ACCAGGGTGA TCATGATGTC CCAGACCTCA CAAACTTTAA AGTTGAAGAA 
CTCAGACAGC GTTATGATAG TGTTATAAAT CGACTATACA CTGGAATTCA GTGCTACTCG 
TGTGGAATGA GATTTACTAC TTCACAGACA GACGTTTATG CAGACCATTT AGATTGGCAC 
TATCGCCAGA ACCGTTCGGA AAAAGATGTT AGCAGAAAAA TTACACACAG AAGGTGGTAC 
TACAGCGTAA CGGATTGGAT TGAGTTTGAA GAAATAGCCG ACTTAGAGGA GCGTGCAAAA 
AGCCAATTTT TTGAAAAAGC TCATGAAGAA GTTGTACTGA AGACACAGGA AGCTGCAAAA 
GAGAAGGAGT TTCAGAGTGT CCCTGCTGGA CCAGCTGGAG CAGTTGAGAG GTGTGAAATC 
TGCCGGGAGC AATTTGAGCA GTACTGGGAT GAGGAAGAGG AGGAGTGGCA TCTAAAGAAT 
GCTATCAGAA TGCATGAAAA GATTTACCAT CCATCCTGTT ATGAGGATTA CCAAAATACA 
TCATCATTTG ATTGCACACC CTCTCCCAGC AAGACCCCTG TAGAAAATCC CCTAAATATT 
ATGTTGAACA TTGTTAAACA GGAAACAGAT GAGTCCTGTG ACTCACCTCA AGTGAAAGAG 
GAACCTGATG ACACCCCTGC TGCCTGTGCA GAAGAGAGCA CACCTGCATC TATAGATGTT 
AAAACAGAAC CTGATGAGTC TGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PCF11 cDNA ORF clone, Gallus gallus(chicken)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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