AMBRA1 cDNA ORF clone, Gallus gallus(chicken)

  • Gene

  • Clones

  • gRNAs

The following AMBRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMBRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa09202 XM_015287216.2
Latest version!
Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa09203 XM_015287217.2
Latest version!
Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa09198 XM_015287215.1
Latest version!
Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa09198 XM_004941601.3
Latest version!
Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa09198 XM_025151377.1
Latest version!
Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa09199 XM_001233288.5
Latest version!
Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OGa09199 XM_001233288.4 Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa09202 XM_015287216.1 Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OGa09198 XM_004941601.2 Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa09203 XM_015287217.1 Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa09202
    Clone ID Related Accession (Same CDS sequence) XM_015287216.2 , XM_015287216.1
    Accession Version XM_015287216.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA AAGGGACAAT
    GGAGATCGAT CCAGGCACCG AGCTCCCCGT AATGCCCGCA TGTCTGCACC ATCGCTGGGG
    CGCTTTGTCC CAAGACGCTT CTTGCTGCCA GAGTACTTGC CTTATGCTGG AATTTTCCAC
    GAAAGGGGAC AGCCTGGCTT GGCCACTCAT TCTTCTGTCA ACAGGGTTTT GGCAGGTGCT
    GTGATTGGAG ACGGACAGTC AGCTGTGGCC AGCAACATTG CCAACACAAC CTACCGGCTC
    CAGTGGTGGG ACTTCACGAA GTTTGACCTG CCAGAAATCA GCAATGCGTC CGTGAACGTG
    CTTGTTCAAA ACTGCAAGAT TTACAATGAT GCTAGCTGTG ATATATCTGC AGATGGGCAG
    CTACTGGCAG CTTTCATTCC CAGCAGCCAG CGAGGCTTCC CCGATGAAGG AATATTGGCT
    GTGTATTCTC TGGCACCCCA TAACTTGGGC GAGATGCTTT ATACAAAACG ATTTGGCCCT
    AATGCCATTT CTGTGAGCCT GTCTCCAATG GGCAGATACG TAATGGTGGG ACTGGCTTCC
    CGGCGTATCC TATTGCACCC TTCCACAGAA CATATGGTTG CTCAAGTCTT CAGGCTGCAA
    CAGCCCCATG GTGGAGAAAC CTCTATGAGG AGAGTTTTCA ATGTTCTGTA CCCAATGCCT
    GCTGACCAGA GAAGACACGT CAGCATAAAC TCTGCTCGCT GGCTACCTGA ACCAGGCCTG
    GGATTAGCAT ATGGCACCAA CAAAGGGGAT CTGGTGATTT GCCGACCAGA GGCCTTAAAC
    TCTGGAGTTG AATACCACTG GGACCAGCAG AATGAGAATG TCTTCACTGT GCATTCCAGC
    AGCAGGAGCA CTGAGAGGCC TGGAACCAGC AGAGCCACGT GGAGGACAGA TAGGGACATG
    GGCCTGATGA ATGCCATAGG TCTACAACCC CGAAACCCCC CCACTTCTGT GACTTCACAG
    GGTACCCAGA CCTTGGCGCC TCAGCTGCAG AATGCCGAGA CTCAGACAGA GAGGGAGGTA
    CAGGAGCAGG AATCCACCTC TGCAGGGACT GGGGAAGGTG AAGGTCCAGA ATATGGGGCC
    AGTGGGGAGG ACGCCGTGAG CAGGATACAA AGGCTGATGG CAGAAGGAGG CATGACTGCC
    GTGGTCCAGC GGGAGCAGAG CACCACCATG GCGTCCATGG GTGGCTTTGG CAACAACATC
    ATCGTGAGTC ACCGGATCCA CCGCAGCTCT CAGACTGGTG CGGAGTCCAC TGGAGCTGAG
    GGCACATCAG CACATCAGTC CACCTCGCAG CAGCTGGCAG CTGAGCTGGA GGGACAGATC
    CTTTCAGAGT CTGTGCAGCT GTCAGAGCAT GGCCTGAGCC CGCGGACATC CTCTGGTGGG
    AGTGAAGGAC GAGGCACTGG TGATCTGGAC CTGCCGGAGC AGGCCCGGTC CTCCATGGAC
    ACTGAGGGAC CAATCGAATA CAGTGACCTA ACTAATAATA ACCATCTTCC TGACAGTACC
    AACTTCTATA GTAATGACAG CACCAGTGGG GAGTCGCGGA ACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142702.1
    CDS283..3030
    Translation

    Target ORF information:

    RefSeq Version XM_015287216.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287216.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA AAGGGACAAT
    GGAGATCGAT CCAGGCACCG AGCTCCCCGT AATGCCCGCA TGTCTGCACC ATCGCTGGGG
    CGCTTTGTCC CAAGACGCTT CTTGCTGCCA GAGTACTTGC CTTATGCTGG AATTTTCCAC
    GAAAGGGGAC AGCCTGGCTT GGCCACTCAT TCTTCTGTCA ACAGGGTTTT GGCAGGTGCT
    GTGATTGGAG ACGGACAGTC AGCTGTGGCC AGCAACATTG CCAACACAAC CTACCGGCTC
    CAGTGGTGGG ACTTCACGAA GTTTGACCTG CCAGAAATCA GCAATGCGTC CGTGAACGTG
    CTTGTTCAAA ACTGCAAGAT TTACAATGAT GCTAGCTGTG ATATATCTGC AGATGGGCAG
    CTACTGGCAG CTTTCATTCC CAGCAGCCAG CGAGGCTTCC CCGATGAAGG AATATTGGCT
    GTGTATTCTC TGGCACCCCA TAACTTGGGC GAGATGCTTT ATACAAAACG ATTTGGCCCT
    AATGCCATTT CTGTGAGCCT GTCTCCAATG GGCAGATACG TAATGGTGGG ACTGGCTTCC
    CGGCGTATCC TATTGCACCC TTCCACAGAA CATATGGTTG CTCAAGTCTT CAGGCTGCAA
    CAGCCCCATG GTGGAGAAAC CTCTATGAGG AGAGTTTTCA ATGTTCTGTA CCCAATGCCT
    GCTGACCAGA GAAGACACGT CAGCATAAAC TCTGCTCGCT GGCTACCTGA ACCAGGCCTG
    GGATTAGCAT ATGGCACCAA CAAAGGGGAT CTGGTGATTT GCCGACCAGA GGCCTTAAAC
    TCTGGAGTTG AATACCACTG GGACCAGCAG AATGAGAATG TCTTCACTGT GCATTCCAGC
    AGCAGGAGCA CTGAGAGGCC TGGAACCAGC AGAGCCACGT GGAGGACAGA TAGGGACATG
    GGCCTGATGA ATGCCATAGG TCTACAACCC CGAAACCCCC CCACTTCTGT GACTTCACAG
    GGTACCCAGA CCTTGGCGCC TCAGCTGCAG AATGCCGAGA CTCAGACAGA GAGGGAGGTA
    CAGGAGCAGG AATCCACCTC TGCAGGGACT GGGGAAGGTG AAGGTCCAGA ATATGGGGCC
    AGTGGGGAGG ACGCCGTGAG CAGGATACAA AGGCTGATGG CAGAAGGAGG CATGACTGCC
    GTGGTCCAGC GGGAGCAGAG CACCACCATG GCGTCCATGG GTGGCTTTGG CAACAACATC
    ATCGTGAGTC ACCGGATCCA CCGCAGCTCT CAGACTGGTG CGGAGTCCAC TGGAGCTGAG
    GGCACATCAG CACATCAGTC CACCTCGCAG CAGCTGGCAG CTGAGCTGGA GGGACAGATC
    CTTTCAGAGT CTGTGCAGCT GTCAGAGCAT GGCCTGAGCC CGCGGACATC CTCTGGTGGG
    AGTGAAGGAC GAGGCACTGG TGATCTGGAC CTGCCGGAGC AGGCCCGGTC CTCCATGGAC
    ACTGAGGGAC CAATCGAATA CAGTGACCTA ACTAATAATA ACCATCTTCC TGACAGTACC
    AACTTCTATA GTAATGACAG CACCAGTGGG GAGTCGCGGA ACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09202
    Clone ID Related Accession (Same CDS sequence) XM_015287216.2 , XM_015287216.1
    Accession Version XM_015287216.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA AAGGGACAAT
    GGAGATCGAT CCAGGCACCG AGCTCCCCGT AATGCCCGCA TGTCTGCACC ATCGCTGGGG
    CGCTTTGTCC CAAGACGCTT CTTGCTGCCA GAGTACTTGC CTTATGCTGG AATTTTCCAC
    GAAAGGGGAC AGCCTGGCTT GGCCACTCAT TCTTCTGTCA ACAGGGTTTT GGCAGGTGCT
    GTGATTGGAG ACGGACAGTC AGCTGTGGCC AGCAACATTG CCAACACAAC CTACCGGCTC
    CAGTGGTGGG ACTTCACGAA GTTTGACCTG CCAGAAATCA GCAATGCGTC CGTGAACGTG
    CTTGTTCAAA ACTGCAAGAT TTACAATGAT GCTAGCTGTG ATATATCTGC AGATGGGCAG
    CTACTGGCAG CTTTCATTCC CAGCAGCCAG CGAGGCTTCC CCGATGAAGG AATATTGGCT
    GTGTATTCTC TGGCACCCCA TAACTTGGGC GAGATGCTTT ATACAAAACG ATTTGGCCCT
    AATGCCATTT CTGTGAGCCT GTCTCCAATG GGCAGATACG TAATGGTGGG ACTGGCTTCC
    CGGCGTATCC TATTGCACCC TTCCACAGAA CATATGGTTG CTCAAGTCTT CAGGCTGCAA
    CAGCCCCATG GTGGAGAAAC CTCTATGAGG AGAGTTTTCA ATGTTCTGTA CCCAATGCCT
    GCTGACCAGA GAAGACACGT CAGCATAAAC TCTGCTCGCT GGCTACCTGA ACCAGGCCTG
    GGATTAGCAT ATGGCACCAA CAAAGGGGAT CTGGTGATTT GCCGACCAGA GGCCTTAAAC
    TCTGGAGTTG AATACCACTG GGACCAGCAG AATGAGAATG TCTTCACTGT GCATTCCAGC
    AGCAGGAGCA CTGAGAGGCC TGGAACCAGC AGAGCCACGT GGAGGACAGA TAGGGACATG
    GGCCTGATGA ATGCCATAGG TCTACAACCC CGAAACCCCC CCACTTCTGT GACTTCACAG
    GGTACCCAGA CCTTGGCGCC TCAGCTGCAG AATGCCGAGA CTCAGACAGA GAGGGAGGTA
    CAGGAGCAGG AATCCACCTC TGCAGGGACT GGGGAAGGTG AAGGTCCAGA ATATGGGGCC
    AGTGGGGAGG ACGCCGTGAG CAGGATACAA AGGCTGATGG CAGAAGGAGG CATGACTGCC
    GTGGTCCAGC GGGAGCAGAG CACCACCATG GCGTCCATGG GTGGCTTTGG CAACAACATC
    ATCGTGAGTC ACCGGATCCA CCGCAGCTCT CAGACTGGTG CGGAGTCCAC TGGAGCTGAG
    GGCACATCAG CACATCAGTC CACCTCGCAG CAGCTGGCAG CTGAGCTGGA GGGACAGATC
    CTTTCAGAGT CTGTGCAGCT GTCAGAGCAT GGCCTGAGCC CGCGGACATC CTCTGGTGGG
    AGTGAAGGAC GAGGCACTGG TGATCTGGAC CTGCCGGAGC AGGCCCGGTC CTCCATGGAC
    ACTGAGGGAC CAATCGAATA CAGTGACCTA ACTAATAATA ACCATCTTCC TGACAGTACC
    AACTTCTATA GTAATGACAG CACCAGTGGG GAGTCGCGGA ACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142702.1
    CDS262..3009
    Translation

    Target ORF information:

    RefSeq Version XM_015287216.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287216.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA AAGGGACAAT
    GGAGATCGAT CCAGGCACCG AGCTCCCCGT AATGCCCGCA TGTCTGCACC ATCGCTGGGG
    CGCTTTGTCC CAAGACGCTT CTTGCTGCCA GAGTACTTGC CTTATGCTGG AATTTTCCAC
    GAAAGGGGAC AGCCTGGCTT GGCCACTCAT TCTTCTGTCA ACAGGGTTTT GGCAGGTGCT
    GTGATTGGAG ACGGACAGTC AGCTGTGGCC AGCAACATTG CCAACACAAC CTACCGGCTC
    CAGTGGTGGG ACTTCACGAA GTTTGACCTG CCAGAAATCA GCAATGCGTC CGTGAACGTG
    CTTGTTCAAA ACTGCAAGAT TTACAATGAT GCTAGCTGTG ATATATCTGC AGATGGGCAG
    CTACTGGCAG CTTTCATTCC CAGCAGCCAG CGAGGCTTCC CCGATGAAGG AATATTGGCT
    GTGTATTCTC TGGCACCCCA TAACTTGGGC GAGATGCTTT ATACAAAACG ATTTGGCCCT
    AATGCCATTT CTGTGAGCCT GTCTCCAATG GGCAGATACG TAATGGTGGG ACTGGCTTCC
    CGGCGTATCC TATTGCACCC TTCCACAGAA CATATGGTTG CTCAAGTCTT CAGGCTGCAA
    CAGCCCCATG GTGGAGAAAC CTCTATGAGG AGAGTTTTCA ATGTTCTGTA CCCAATGCCT
    GCTGACCAGA GAAGACACGT CAGCATAAAC TCTGCTCGCT GGCTACCTGA ACCAGGCCTG
    GGATTAGCAT ATGGCACCAA CAAAGGGGAT CTGGTGATTT GCCGACCAGA GGCCTTAAAC
    TCTGGAGTTG AATACCACTG GGACCAGCAG AATGAGAATG TCTTCACTGT GCATTCCAGC
    AGCAGGAGCA CTGAGAGGCC TGGAACCAGC AGAGCCACGT GGAGGACAGA TAGGGACATG
    GGCCTGATGA ATGCCATAGG TCTACAACCC CGAAACCCCC CCACTTCTGT GACTTCACAG
    GGTACCCAGA CCTTGGCGCC TCAGCTGCAG AATGCCGAGA CTCAGACAGA GAGGGAGGTA
    CAGGAGCAGG AATCCACCTC TGCAGGGACT GGGGAAGGTG AAGGTCCAGA ATATGGGGCC
    AGTGGGGAGG ACGCCGTGAG CAGGATACAA AGGCTGATGG CAGAAGGAGG CATGACTGCC
    GTGGTCCAGC GGGAGCAGAG CACCACCATG GCGTCCATGG GTGGCTTTGG CAACAACATC
    ATCGTGAGTC ACCGGATCCA CCGCAGCTCT CAGACTGGTG CGGAGTCCAC TGGAGCTGAG
    GGCACATCAG CACATCAGTC CACCTCGCAG CAGCTGGCAG CTGAGCTGGA GGGACAGATC
    CTTTCAGAGT CTGTGCAGCT GTCAGAGCAT GGCCTGAGCC CGCGGACATC CTCTGGTGGG
    AGTGAAGGAC GAGGCACTGG TGATCTGGAC CTGCCGGAGC AGGCCCGGTC CTCCATGGAC
    ACTGAGGGAC CAATCGAATA CAGTGACCTA ACTAATAATA ACCATCTTCC TGACAGTACC
    AACTTCTATA GTAATGACAG CACCAGTGGG GAGTCGCGGA ACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09203
    Clone ID Related Accession (Same CDS sequence) XM_015287217.2 , XM_015287217.1
    Accession Version XM_015287217.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287217.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA GGACAATGGA
    GATCGATCCA GGCACCGAGC TCCCCGTAAT GCCCGCATGT CTGCACCATC GCTGGGGCGC
    TTTGTCCCAA GACGCTTCTT GCTGCCAGAG TACTTGCCTT ATGCTGGAAT TTTCCACGAA
    AGGGGACAGC CTGGCTTGGC CACTCATTCT TCTGTCAACA GGGTTTTGGC AGGTGCTGTG
    ATTGGAGACG GACAGTCAGC TGTGGCCAGC AACATTGCCA ACACAACCTA CCGGCTCCAG
    TGGTGGGACT TCACGAAGTT TGACCTGCCA GAAATCAGCA ATGCGTCCGT GAACGTGCTT
    GTTCAAAACT GCAAGATTTA CAATGATGCT AGCTGTGATA TATCTGCAGA TGGGCAGCTA
    CTGGCAGCTT TCATTCCCAG CAGCCAGCGA GGCTTCCCCG ATGAAGGAAT ATTGGCTGTG
    TATTCTCTGG CACCCCATAA CTTGGGCGAG ATGCTTTATA CAAAACGATT TGGCCCTAAT
    GCCATTTCTG TGAGCCTGTC TCCAATGGGC AGATACGTAA TGGTGGGACT GGCTTCCCGG
    CGTATCCTAT TGCACCCTTC CACAGAACAT ATGGTTGCTC AAGTCTTCAG GCTGCAACAG
    CCCCATGGTG GAGAAACCTC TATGAGGAGA GTTTTCAATG TTCTGTACCC AATGCCTGCT
    GACCAGAGAA GACACGTCAG CATAAACTCT GCTCGCTGGC TACCTGAACC AGGCCTGGGA
    TTAGCATATG GCACCAACAA AGGGGATCTG GTGATTTGCC GACCAGAGGC CTTAAACTCT
    GGAGTTGAAT ACCACTGGGA CCAGCAGAAT GAGAATGTCT TCACTGTGCA TTCCAGCAGC
    AGGAGCACTG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGATAG GGACATGGGC
    CTGATGAATG CCATAGGTCT ACAACCCCGA AACCCCCCCA CTTCTGTGAC TTCACAGGGT
    ACCCAGACCT TGGCGCCTCA GCTGCAGAAT GCCGAGACTC AGACAGAGAG GGAGGTACAG
    GAGCAGGAAT CCACCTCTGC AGGGACTGGG GAAGGTGAAG GTCCAGAATA TGGGGCCAGT
    GGGGAGGACG CCGTGAGCAG GATACAAAGG CTGATGGCAG AAGGAGGCAT GACTGCCGTG
    GTCCAGCGGG AGCAGAGCAC CACCATGGCG TCCATGGGTG GCTTTGGCAA CAACATCATC
    GTGAGTCACC GGATCCACCG CAGCTCTCAG ACTGGTGCGG AGTCCACTGG AGCTGAGGGC
    ACATCAGCAC ATCAGTCCAC CTCGCAGCAG CTGGCAGCTG AGCTGGAGGG ACAGATCCTT
    TCAGAGTCTG TGCAGCTGTC AGAGCATGGC CTGAGCCCGC GGACATCCTC TGGTGGGAGT
    GAAGGACGAG GCACTGGTGA TCTGGACCTG CCGGAGCAGG CCCGGTCCTC CATGGACACT
    GAGGGACCAA TCGAATACAG TGACCTAACT AATAATAACC ATCTTCCTGA CAGTACCAAC
    TTCTATAGTA ATGACAGCAC CAGTGGGGAG TCGCGGAACA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142703.1
    CDS283..3027
    Translation

    Target ORF information:

    RefSeq Version XM_015287217.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287217.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA GGACAATGGA
    GATCGATCCA GGCACCGAGC TCCCCGTAAT GCCCGCATGT CTGCACCATC GCTGGGGCGC
    TTTGTCCCAA GACGCTTCTT GCTGCCAGAG TACTTGCCTT ATGCTGGAAT TTTCCACGAA
    AGGGGACAGC CTGGCTTGGC CACTCATTCT TCTGTCAACA GGGTTTTGGC AGGTGCTGTG
    ATTGGAGACG GACAGTCAGC TGTGGCCAGC AACATTGCCA ACACAACCTA CCGGCTCCAG
    TGGTGGGACT TCACGAAGTT TGACCTGCCA GAAATCAGCA ATGCGTCCGT GAACGTGCTT
    GTTCAAAACT GCAAGATTTA CAATGATGCT AGCTGTGATA TATCTGCAGA TGGGCAGCTA
    CTGGCAGCTT TCATTCCCAG CAGCCAGCGA GGCTTCCCCG ATGAAGGAAT ATTGGCTGTG
    TATTCTCTGG CACCCCATAA CTTGGGCGAG ATGCTTTATA CAAAACGATT TGGCCCTAAT
    GCCATTTCTG TGAGCCTGTC TCCAATGGGC AGATACGTAA TGGTGGGACT GGCTTCCCGG
    CGTATCCTAT TGCACCCTTC CACAGAACAT ATGGTTGCTC AAGTCTTCAG GCTGCAACAG
    CCCCATGGTG GAGAAACCTC TATGAGGAGA GTTTTCAATG TTCTGTACCC AATGCCTGCT
    GACCAGAGAA GACACGTCAG CATAAACTCT GCTCGCTGGC TACCTGAACC AGGCCTGGGA
    TTAGCATATG GCACCAACAA AGGGGATCTG GTGATTTGCC GACCAGAGGC CTTAAACTCT
    GGAGTTGAAT ACCACTGGGA CCAGCAGAAT GAGAATGTCT TCACTGTGCA TTCCAGCAGC
    AGGAGCACTG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGATAG GGACATGGGC
    CTGATGAATG CCATAGGTCT ACAACCCCGA AACCCCCCCA CTTCTGTGAC TTCACAGGGT
    ACCCAGACCT TGGCGCCTCA GCTGCAGAAT GCCGAGACTC AGACAGAGAG GGAGGTACAG
    GAGCAGGAAT CCACCTCTGC AGGGACTGGG GAAGGTGAAG GTCCAGAATA TGGGGCCAGT
    GGGGAGGACG CCGTGAGCAG GATACAAAGG CTGATGGCAG AAGGAGGCAT GACTGCCGTG
    GTCCAGCGGG AGCAGAGCAC CACCATGGCG TCCATGGGTG GCTTTGGCAA CAACATCATC
    GTGAGTCACC GGATCCACCG CAGCTCTCAG ACTGGTGCGG AGTCCACTGG AGCTGAGGGC
    ACATCAGCAC ATCAGTCCAC CTCGCAGCAG CTGGCAGCTG AGCTGGAGGG ACAGATCCTT
    TCAGAGTCTG TGCAGCTGTC AGAGCATGGC CTGAGCCCGC GGACATCCTC TGGTGGGAGT
    GAAGGACGAG GCACTGGTGA TCTGGACCTG CCGGAGCAGG CCCGGTCCTC CATGGACACT
    GAGGGACCAA TCGAATACAG TGACCTAACT AATAATAACC ATCTTCCTGA CAGTACCAAC
    TTCTATAGTA ATGACAGCAC CAGTGGGGAG TCGCGGAACA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09203
    Clone ID Related Accession (Same CDS sequence) XM_015287217.2 , XM_015287217.1
    Accession Version XM_015287217.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA GGACAATGGA
    GATCGATCCA GGCACCGAGC TCCCCGTAAT GCCCGCATGT CTGCACCATC GCTGGGGCGC
    TTTGTCCCAA GACGCTTCTT GCTGCCAGAG TACTTGCCTT ATGCTGGAAT TTTCCACGAA
    AGGGGACAGC CTGGCTTGGC CACTCATTCT TCTGTCAACA GGGTTTTGGC AGGTGCTGTG
    ATTGGAGACG GACAGTCAGC TGTGGCCAGC AACATTGCCA ACACAACCTA CCGGCTCCAG
    TGGTGGGACT TCACGAAGTT TGACCTGCCA GAAATCAGCA ATGCGTCCGT GAACGTGCTT
    GTTCAAAACT GCAAGATTTA CAATGATGCT AGCTGTGATA TATCTGCAGA TGGGCAGCTA
    CTGGCAGCTT TCATTCCCAG CAGCCAGCGA GGCTTCCCCG ATGAAGGAAT ATTGGCTGTG
    TATTCTCTGG CACCCCATAA CTTGGGCGAG ATGCTTTATA CAAAACGATT TGGCCCTAAT
    GCCATTTCTG TGAGCCTGTC TCCAATGGGC AGATACGTAA TGGTGGGACT GGCTTCCCGG
    CGTATCCTAT TGCACCCTTC CACAGAACAT ATGGTTGCTC AAGTCTTCAG GCTGCAACAG
    CCCCATGGTG GAGAAACCTC TATGAGGAGA GTTTTCAATG TTCTGTACCC AATGCCTGCT
    GACCAGAGAA GACACGTCAG CATAAACTCT GCTCGCTGGC TACCTGAACC AGGCCTGGGA
    TTAGCATATG GCACCAACAA AGGGGATCTG GTGATTTGCC GACCAGAGGC CTTAAACTCT
    GGAGTTGAAT ACCACTGGGA CCAGCAGAAT GAGAATGTCT TCACTGTGCA TTCCAGCAGC
    AGGAGCACTG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGATAG GGACATGGGC
    CTGATGAATG CCATAGGTCT ACAACCCCGA AACCCCCCCA CTTCTGTGAC TTCACAGGGT
    ACCCAGACCT TGGCGCCTCA GCTGCAGAAT GCCGAGACTC AGACAGAGAG GGAGGTACAG
    GAGCAGGAAT CCACCTCTGC AGGGACTGGG GAAGGTGAAG GTCCAGAATA TGGGGCCAGT
    GGGGAGGACG CCGTGAGCAG GATACAAAGG CTGATGGCAG AAGGAGGCAT GACTGCCGTG
    GTCCAGCGGG AGCAGAGCAC CACCATGGCG TCCATGGGTG GCTTTGGCAA CAACATCATC
    GTGAGTCACC GGATCCACCG CAGCTCTCAG ACTGGTGCGG AGTCCACTGG AGCTGAGGGC
    ACATCAGCAC ATCAGTCCAC CTCGCAGCAG CTGGCAGCTG AGCTGGAGGG ACAGATCCTT
    TCAGAGTCTG TGCAGCTGTC AGAGCATGGC CTGAGCCCGC GGACATCCTC TGGTGGGAGT
    GAAGGACGAG GCACTGGTGA TCTGGACCTG CCGGAGCAGG CCCGGTCCTC CATGGACACT
    GAGGGACCAA TCGAATACAG TGACCTAACT AATAATAACC ATCTTCCTGA CAGTACCAAC
    TTCTATAGTA ATGACAGCAC CAGTGGGGAG TCGCGGAACA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142703.1
    CDS261..3005
    Translation

    Target ORF information:

    RefSeq Version XM_015287217.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGAGGA GAGGACCGTG
    GGAGTGGTCT TTAACCAGGA GACGGGGCAC TGGGAGAGAG TTTACAGCCA GTCGGCTTCA
    AGCAGACCTG GGAATGTATC ACAGGAGGCC TTAAACCAGG AAATGCCTGA GGAAAGCTCA
    GAGGAGGATT CACTCAGGAG GAGATTGCTG GAGTCTTCCC TCATTTCGTT ATCCCGCTAT
    GACGGAGCAG GGTCCCGGGA ACATCCAATC TATCCAGATC CAGCCAGGTT ATCTCCTGCT
    GCATATTATG CTCAGAGGAT GATTCAGTAT CTTTCTCGGA GGGACAGTAT TCGACAGCGA
    TCCATGCGAT ACCAACAGAA CCGTCTCCGC TCTTCCTCCT CCTCTTCTTC CACTTCGGAG
    AACTCAAGCC CATCTGTAGA GGGAAATGAC CTGGAATTTG AAGATTTTGA GGACAATGGA
    GATCGATCCA GGCACCGAGC TCCCCGTAAT GCCCGCATGT CTGCACCATC GCTGGGGCGC
    TTTGTCCCAA GACGCTTCTT GCTGCCAGAG TACTTGCCTT ATGCTGGAAT TTTCCACGAA
    AGGGGACAGC CTGGCTTGGC CACTCATTCT TCTGTCAACA GGGTTTTGGC AGGTGCTGTG
    ATTGGAGACG GACAGTCAGC TGTGGCCAGC AACATTGCCA ACACAACCTA CCGGCTCCAG
    TGGTGGGACT TCACGAAGTT TGACCTGCCA GAAATCAGCA ATGCGTCCGT GAACGTGCTT
    GTTCAAAACT GCAAGATTTA CAATGATGCT AGCTGTGATA TATCTGCAGA TGGGCAGCTA
    CTGGCAGCTT TCATTCCCAG CAGCCAGCGA GGCTTCCCCG ATGAAGGAAT ATTGGCTGTG
    TATTCTCTGG CACCCCATAA CTTGGGCGAG ATGCTTTATA CAAAACGATT TGGCCCTAAT
    GCCATTTCTG TGAGCCTGTC TCCAATGGGC AGATACGTAA TGGTGGGACT GGCTTCCCGG
    CGTATCCTAT TGCACCCTTC CACAGAACAT ATGGTTGCTC AAGTCTTCAG GCTGCAACAG
    CCCCATGGTG GAGAAACCTC TATGAGGAGA GTTTTCAATG TTCTGTACCC AATGCCTGCT
    GACCAGAGAA GACACGTCAG CATAAACTCT GCTCGCTGGC TACCTGAACC AGGCCTGGGA
    TTAGCATATG GCACCAACAA AGGGGATCTG GTGATTTGCC GACCAGAGGC CTTAAACTCT
    GGAGTTGAAT ACCACTGGGA CCAGCAGAAT GAGAATGTCT TCACTGTGCA TTCCAGCAGC
    AGGAGCACTG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGATAG GGACATGGGC
    CTGATGAATG CCATAGGTCT ACAACCCCGA AACCCCCCCA CTTCTGTGAC TTCACAGGGT
    ACCCAGACCT TGGCGCCTCA GCTGCAGAAT GCCGAGACTC AGACAGAGAG GGAGGTACAG
    GAGCAGGAAT CCACCTCTGC AGGGACTGGG GAAGGTGAAG GTCCAGAATA TGGGGCCAGT
    GGGGAGGACG CCGTGAGCAG GATACAAAGG CTGATGGCAG AAGGAGGCAT GACTGCCGTG
    GTCCAGCGGG AGCAGAGCAC CACCATGGCG TCCATGGGTG GCTTTGGCAA CAACATCATC
    GTGAGTCACC GGATCCACCG CAGCTCTCAG ACTGGTGCGG AGTCCACTGG AGCTGAGGGC
    ACATCAGCAC ATCAGTCCAC CTCGCAGCAG CTGGCAGCTG AGCTGGAGGG ACAGATCCTT
    TCAGAGTCTG TGCAGCTGTC AGAGCATGGC CTGAGCCCGC GGACATCCTC TGGTGGGAGT
    GAAGGACGAG GCACTGGTGA TCTGGACCTG CCGGAGCAGG CCCGGTCCTC CATGGACACT
    GAGGGACCAA TCGAATACAG TGACCTAACT AATAATAACC ATCTTCCTGA CAGTACCAAC
    TTCTATAGTA ATGACAGCAC CAGTGGGGAG TCGCGGAACA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09198
    Clone ID Related Accession (Same CDS sequence) XM_015287215.1 , XM_004941601.3 , XM_025151377.1 , XM_004941601.2
    Accession Version XM_015287215.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142701.1
    CDS384..4289
    Translation

    Target ORF information:

    RefSeq Version XM_015287215.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287215.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09198
    Clone ID Related Accession (Same CDS sequence) XM_015287215.1 , XM_004941601.3 , XM_025151377.1 , XM_004941601.2
    Accession Version XM_004941601.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941658.1
    CDS283..4188
    Translation

    Target ORF information:

    RefSeq Version XM_004941601.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941601.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09198
    Clone ID Related Accession (Same CDS sequence) XM_015287215.1 , XM_004941601.3 , XM_025151377.1 , XM_004941601.2
    Accession Version XM_025151377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007145.1
    CDS245..4150
    Translation

    Target ORF information:

    RefSeq Version XM_025151377.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025151377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09198
    Clone ID Related Accession (Same CDS sequence) XM_015287215.1 , XM_004941601.3 , XM_025151377.1 , XM_004941601.2
    Accession Version XM_004941601.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941658.1
    CDS265..4170
    Translation

    Target ORF information:

    RefSeq Version XM_004941601.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941601.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAAA GGGACAATGG AGATCGATCC AGGCACCGAG CTCCCCGTAA TGCCCGCATG
    TCTGCACCAT CGCTGGGGCG CTTTGTCCCA AGACGCTTCT TGCTGCCAGA GTACTTGCCT
    TATGCTGGAA TTTTCCACGA AAGGGGACAG CCTGGCTTGG CCACTCATTC TTCTGTCAAC
    AGGGTTTTGG CAGGTGCTGT GATTGGAGAC GGACAGTCAG CTGTGGCCAG CAACATTGCC
    AACACAACCT ACCGGCTCCA GTGGTGGGAC TTCACGAAGT TTGACCTGCC AGAAATCAGC
    AATGCGTCCG TGAACGTGCT TGTTCAAAAC TGCAAGATTT ACAATGATGC TAGCTGTGAT
    ATATCTGCAG ATGGGCAGCT ACTGGCAGCT TTCATTCCCA GCAGCCAGCG AGGCTTCCCC
    GATGAAGGAA TATTGGCTGT GTATTCTCTG GCACCCCATA ACTTGGGCGA GATGCTTTAT
    ACAAAACGAT TTGGCCCTAA TGCCATTTCT GTGAGCCTGT CTCCAATGGG CAGATACGTA
    ATGGTGGGAC TGGCTTCCCG GCGTATCCTA TTGCACCCTT CCACAGAACA TATGGTTGCT
    CAAGTCTTCA GGCTGCAACA GCCCCATGGT GGAGAAACCT CTATGAGGAG AGTTTTCAAT
    GTTCTGTACC CAATGCCTGC TGACCAGAGA AGACACGTCA GCATAAACTC TGCTCGCTGG
    CTACCTGAAC CAGGCCTGGG ATTAGCATAT GGCACCAACA AAGGGGATCT GGTGATTTGC
    CGACCAGAGG CCTTAAACTC TGGAGTTGAA TACCACTGGG ACCAGCAGAA TGAGAATGTC
    TTCACTGTGC ATTCCAGCAG CAGGAGCACT GAGAGGCCTG GAACCAGCAG AGCCACGTGG
    AGGACAGATA GGGACATGGG CCTGATGAAT GCCATAGGTC TACAACCCCG AAACCCCCCC
    ACTTCTGTGA CTTCACAGGG TACCCAGACC TTGGCGCCTC AGCTGCAGAA TGCCGAGACT
    CAGACAGAGA GGGAGGTACA GGAGCAGGAA TCCACCTCTG CAGGGACTGG GGAAGGTGAA
    GGTCCAGAAT ATGGGGCCAG TGGGGAGGAC GCCGTGAGCA GGATACAAAG GCTGATGGCA
    GAAGGAGGCA TGACTGCCGT GGTCCAGCGG GAGCAGAGCA CCACCATGGC GTCCATGGGT
    GGCTTTGGCA ACAACATCAT CGTGAGTCAC CGGATCCACC GCAGCTCTCA GACTGGTGCG
    GAGTCCACTG GAGCTGAGGG CACATCAGCA CATCAGTCCA CCTCGCAGCA GCTGGCAGCT
    GAGCTGGAGG GACAGATCCT TTCAGAGTCT GTGCAGCTGT CAGAGCATGG CCTGAGCCCG
    CGGACATCCT CTGGTGGGAG TGAAGGACGA GGCACTGGTG ATCTGGACCT GCCGGAGCAG
    GCCCGGTCCT CCATGGACAC TGAGGGACCA ATCGAATACA GTGACCTAAC TAATAATAAC
    CATCTTCCTG ACAGTACCAA CTTCTATAGT AATGACAGCA CCAGTGGGGA GTCGCGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09199
    Clone ID Related Accession (Same CDS sequence) XM_001233288.5 , XM_001233288.4
    Accession Version XM_001233288.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_001233288.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAGG ACAATGGAGA TCGATCCAGG CACCGAGCTC CCCGTAATGC CCGCATGTCT
    GCACCATCGC TGGGGCGCTT TGTCCCAAGA CGCTTCTTGC TGCCAGAGTA CTTGCCTTAT
    GCTGGAATTT TCCACGAAAG GGGACAGCCT GGCTTGGCCA CTCATTCTTC TGTCAACAGG
    GTTTTGGCAG GTGCTGTGAT TGGAGACGGA CAGTCAGCTG TGGCCAGCAA CATTGCCAAC
    ACAACCTACC GGCTCCAGTG GTGGGACTTC ACGAAGTTTG ACCTGCCAGA AATCAGCAAT
    GCGTCCGTGA ACGTGCTTGT TCAAAACTGC AAGATTTACA ATGATGCTAG CTGTGATATA
    TCTGCAGATG GGCAGCTACT GGCAGCTTTC ATTCCCAGCA GCCAGCGAGG CTTCCCCGAT
    GAAGGAATAT TGGCTGTGTA TTCTCTGGCA CCCCATAACT TGGGCGAGAT GCTTTATACA
    AAACGATTTG GCCCTAATGC CATTTCTGTG AGCCTGTCTC CAATGGGCAG ATACGTAATG
    GTGGGACTGG CTTCCCGGCG TATCCTATTG CACCCTTCCA CAGAACATAT GGTTGCTCAA
    GTCTTCAGGC TGCAACAGCC CCATGGTGGA GAAACCTCTA TGAGGAGAGT TTTCAATGTT
    CTGTACCCAA TGCCTGCTGA CCAGAGAAGA CACGTCAGCA TAAACTCTGC TCGCTGGCTA
    CCTGAACCAG GCCTGGGATT AGCATATGGC ACCAACAAAG GGGATCTGGT GATTTGCCGA
    CCAGAGGCCT TAAACTCTGG AGTTGAATAC CACTGGGACC AGCAGAATGA GAATGTCTTC
    ACTGTGCATT CCAGCAGCAG GAGCACTGAG AGGCCTGGAA CCAGCAGAGC CACGTGGAGG
    ACAGATAGGG ACATGGGCCT GATGAATGCC ATAGGTCTAC AACCCCGAAA CCCCCCCACT
    TCTGTGACTT CACAGGGTAC CCAGACCTTG GCGCCTCAGC TGCAGAATGC CGAGACTCAG
    ACAGAGAGGG AGGTACAGGA GCAGGAATCC ACCTCTGCAG GGACTGGGGA AGGTGAAGGT
    CCAGAATATG GGGCCAGTGG GGAGGACGCC GTGAGCAGGA TACAAAGGCT GATGGCAGAA
    GGAGGCATGA CTGCCGTGGT CCAGCGGGAG CAGAGCACCA CCATGGCGTC CATGGGTGGC
    TTTGGCAACA ACATCATCGT GAGTCACCGG ATCCACCGCA GCTCTCAGAC TGGTGCGGAG
    TCCACTGGAG CTGAGGGCAC ATCAGCACAT CAGTCCACCT CGCAGCAGCT GGCAGCTGAG
    CTGGAGGGAC AGATCCTTTC AGAGTCTGTG CAGCTGTCAG AGCATGGCCT GAGCCCGCGG
    ACATCCTCTG GTGGGAGTGA AGGACGAGGC ACTGGTGATC TGGACCTGCC GGAGCAGGCC
    CGGTCCTCCA TGGACACTGA GGGACCAATC GAATACAGTG ACCTAACTAA TAATAACCAT
    CTTCCTGACA GTACCAACTT CTATAGTAAT GACAGCACCA GTGGGGAGTC GCGGAACAGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001233289.3
    CDS283..4185
    Translation

    Target ORF information:

    RefSeq Version XM_001233288.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001233288.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAGG ACAATGGAGA TCGATCCAGG CACCGAGCTC CCCGTAATGC CCGCATGTCT
    GCACCATCGC TGGGGCGCTT TGTCCCAAGA CGCTTCTTGC TGCCAGAGTA CTTGCCTTAT
    GCTGGAATTT TCCACGAAAG GGGACAGCCT GGCTTGGCCA CTCATTCTTC TGTCAACAGG
    GTTTTGGCAG GTGCTGTGAT TGGAGACGGA CAGTCAGCTG TGGCCAGCAA CATTGCCAAC
    ACAACCTACC GGCTCCAGTG GTGGGACTTC ACGAAGTTTG ACCTGCCAGA AATCAGCAAT
    GCGTCCGTGA ACGTGCTTGT TCAAAACTGC AAGATTTACA ATGATGCTAG CTGTGATATA
    TCTGCAGATG GGCAGCTACT GGCAGCTTTC ATTCCCAGCA GCCAGCGAGG CTTCCCCGAT
    GAAGGAATAT TGGCTGTGTA TTCTCTGGCA CCCCATAACT TGGGCGAGAT GCTTTATACA
    AAACGATTTG GCCCTAATGC CATTTCTGTG AGCCTGTCTC CAATGGGCAG ATACGTAATG
    GTGGGACTGG CTTCCCGGCG TATCCTATTG CACCCTTCCA CAGAACATAT GGTTGCTCAA
    GTCTTCAGGC TGCAACAGCC CCATGGTGGA GAAACCTCTA TGAGGAGAGT TTTCAATGTT
    CTGTACCCAA TGCCTGCTGA CCAGAGAAGA CACGTCAGCA TAAACTCTGC TCGCTGGCTA
    CCTGAACCAG GCCTGGGATT AGCATATGGC ACCAACAAAG GGGATCTGGT GATTTGCCGA
    CCAGAGGCCT TAAACTCTGG AGTTGAATAC CACTGGGACC AGCAGAATGA GAATGTCTTC
    ACTGTGCATT CCAGCAGCAG GAGCACTGAG AGGCCTGGAA CCAGCAGAGC CACGTGGAGG
    ACAGATAGGG ACATGGGCCT GATGAATGCC ATAGGTCTAC AACCCCGAAA CCCCCCCACT
    TCTGTGACTT CACAGGGTAC CCAGACCTTG GCGCCTCAGC TGCAGAATGC CGAGACTCAG
    ACAGAGAGGG AGGTACAGGA GCAGGAATCC ACCTCTGCAG GGACTGGGGA AGGTGAAGGT
    CCAGAATATG GGGCCAGTGG GGAGGACGCC GTGAGCAGGA TACAAAGGCT GATGGCAGAA
    GGAGGCATGA CTGCCGTGGT CCAGCGGGAG CAGAGCACCA CCATGGCGTC CATGGGTGGC
    TTTGGCAACA ACATCATCGT GAGTCACCGG ATCCACCGCA GCTCTCAGAC TGGTGCGGAG
    TCCACTGGAG CTGAGGGCAC ATCAGCACAT CAGTCCACCT CGCAGCAGCT GGCAGCTGAG
    CTGGAGGGAC AGATCCTTTC AGAGTCTGTG CAGCTGTCAG AGCATGGCCT GAGCCCGCGG
    ACATCCTCTG GTGGGAGTGA AGGACGAGGC ACTGGTGATC TGGACCTGCC GGAGCAGGCC
    CGGTCCTCCA TGGACACTGA GGGACCAATC GAATACAGTG ACCTAACTAA TAATAACCAT
    CTTCCTGACA GTACCAACTT CTATAGTAAT GACAGCACCA GTGGGGAGTC GCGGAACAGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09199
    Clone ID Related Accession (Same CDS sequence) XM_001233288.5 , XM_001233288.4
    Accession Version XM_001233288.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_001233288.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAGG ACAATGGAGA TCGATCCAGG CACCGAGCTC CCCGTAATGC CCGCATGTCT
    GCACCATCGC TGGGGCGCTT TGTCCCAAGA CGCTTCTTGC TGCCAGAGTA CTTGCCTTAT
    GCTGGAATTT TCCACGAAAG GGGACAGCCT GGCTTGGCCA CTCATTCTTC TGTCAACAGG
    GTTTTGGCAG GTGCTGTGAT TGGAGACGGA CAGTCAGCTG TGGCCAGCAA CATTGCCAAC
    ACAACCTACC GGCTCCAGTG GTGGGACTTC ACGAAGTTTG ACCTGCCAGA AATCAGCAAT
    GCGTCCGTGA ACGTGCTTGT TCAAAACTGC AAGATTTACA ATGATGCTAG CTGTGATATA
    TCTGCAGATG GGCAGCTACT GGCAGCTTTC ATTCCCAGCA GCCAGCGAGG CTTCCCCGAT
    GAAGGAATAT TGGCTGTGTA TTCTCTGGCA CCCCATAACT TGGGCGAGAT GCTTTATACA
    AAACGATTTG GCCCTAATGC CATTTCTGTG AGCCTGTCTC CAATGGGCAG ATACGTAATG
    GTGGGACTGG CTTCCCGGCG TATCCTATTG CACCCTTCCA CAGAACATAT GGTTGCTCAA
    GTCTTCAGGC TGCAACAGCC CCATGGTGGA GAAACCTCTA TGAGGAGAGT TTTCAATGTT
    CTGTACCCAA TGCCTGCTGA CCAGAGAAGA CACGTCAGCA TAAACTCTGC TCGCTGGCTA
    CCTGAACCAG GCCTGGGATT AGCATATGGC ACCAACAAAG GGGATCTGGT GATTTGCCGA
    CCAGAGGCCT TAAACTCTGG AGTTGAATAC CACTGGGACC AGCAGAATGA GAATGTCTTC
    ACTGTGCATT CCAGCAGCAG GAGCACTGAG AGGCCTGGAA CCAGCAGAGC CACGTGGAGG
    ACAGATAGGG ACATGGGCCT GATGAATGCC ATAGGTCTAC AACCCCGAAA CCCCCCCACT
    TCTGTGACTT CACAGGGTAC CCAGACCTTG GCGCCTCAGC TGCAGAATGC CGAGACTCAG
    ACAGAGAGGG AGGTACAGGA GCAGGAATCC ACCTCTGCAG GGACTGGGGA AGGTGAAGGT
    CCAGAATATG GGGCCAGTGG GGAGGACGCC GTGAGCAGGA TACAAAGGCT GATGGCAGAA
    GGAGGCATGA CTGCCGTGGT CCAGCGGGAG CAGAGCACCA CCATGGCGTC CATGGGTGGC
    TTTGGCAACA ACATCATCGT GAGTCACCGG ATCCACCGCA GCTCTCAGAC TGGTGCGGAG
    TCCACTGGAG CTGAGGGCAC ATCAGCACAT CAGTCCACCT CGCAGCAGCT GGCAGCTGAG
    CTGGAGGGAC AGATCCTTTC AGAGTCTGTG CAGCTGTCAG AGCATGGCCT GAGCCCGCGG
    ACATCCTCTG GTGGGAGTGA AGGACGAGGC ACTGGTGATC TGGACCTGCC GGAGCAGGCC
    CGGTCCTCCA TGGACACTGA GGGACCAATC GAATACAGTG ACCTAACTAA TAATAACCAT
    CTTCCTGACA GTACCAACTT CTATAGTAAT GACAGCACCA GTGGGGAGTC GCGGAACAGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001233289.3
    CDS229..4131
    Translation

    Target ORF information:

    RefSeq Version XM_001233288.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001233288.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TACCGGAGAA GAATGCGGTC CGCATCCTGT GGGGTAGGGA ACGAGGTACC 
    CAGGCACTGG GAGCTCAGCG GCTCCTGCAG GAGTTGGTGG AGGATAAAAC TCGATGGATG
    AAATGGGAGG GCAAGAAAGT TGAGTTACCA GACAGTCCAC GCTCCACCTT CTTGCTGGCG
    TTTAGTCCAG ACAGGACCCT AATGGCTTCC ACACATGTGA ACCACAACAT CTACATCACA
    GAGGTGAAAA CGGGCAAGTG TGTTCATTCT TTGGTTGGAC ATCGCCGCAC TCCCTGGTGT
    GTGACCTTCC ATCCCACCAT TCCAGGCCTC ATTGCTTCAG GATGCCTAGA TGGGGAGGTT
    AGAATTTGGG ATCTACATGG TGGAAGTGAA AGCTGGTTCA CGGATAGCAA CAATGCCATT
    GCATCGCTCG CTTTTCATCC TACGGCTCAG CTCTTGCTGA TTGCAACGGC CAATGAGATA
    CATTTCTGGG ACTGGAGCCG AAGAGAGCCT TTTGCAGTGG TGAAAACAGC CAGCGAAATG
    GAGCGGGTCC GGCTAGTACG GTTTGATCCC TTGGGACACT ACTTGCTCAC GGCAATTGTT
    AACCCTTCCA ACCAGCAGAG CGACGAGGAA GTGGAAATCT CCGTGGACAG CACAGAGATG
    CCGCATTATC GCCAGCGCGC TATCCTTCCC TCTCAGCCTG TGAGGCGCAC ACCTCTCCTC
    CACAACTTCC TGCATATGTT GTCCTCCCGC TCCTCTGGCA TACAGGTGGG AGAGCAAAAC
    AGTGTTCAAG ACTCTGCTAC CCCATCCCCT CCACCGCCTC CACCACCACC ACCTCCGCCT
    CCAGCCAGTG AGAACACAAG GGCATCTGTT TATAACAGGC TCCGGGAGCG TGTCAGCTAC
    CCCACAGCAG AGTGCTGCCA GCACTTGGGG ATGTTGTGCC TTTGCAGCCG ATGCTCTAGT
    GCAAGACTTC CGTCTTCTCT CTTCCCACAC CAGGAAAACG CACCCTCCAC GTCTTCTGGG
    GCCACTGGCA CGTCCTTCTC CTCTGTGCAG ACAGAGCCTT ACCAGCCCCC AGAGCAGGCA
    TCTGTAGCAC AGGAGGAGCA GGGGATACTT AACAGGCCCT CTGCTTTCAG CACAGTTCAG
    AGCAGCGCTG CTGGCAACAC CCTGCGCAAC CTTAGCCTGG GCCCCACGCG GCGGTCCCTG
    AGCGGGCCCT TGGCAGGCCA CCAGTCCCGC TACCAGCAGT CTGCAAGAGA GATGGCTTCG
    GGCTTGGGCG GGTCTGACTG GTCCAGGACA GTGCTGAACA TGAGCTCCCG GTCAGAGCTG
    GAAGCCATGC CTCCCCCTAG GACCAGTGCC TCCTCCGTGA GCTTGCTGTC GGTGCTGCGG
    CAGCAGGAGG GAGGTTCCCA GGCATCGGTC TATACCTCTG CAACAGAAGG GAGAGGCTTC
    CTAGCCCCAG GGGCTGAGGC GGACAGTGGT AGTGGCACTG GCCCGAGCAA TCCGGCCAGC
    ATCCGTAATG AGCTCCAGTG TGATTTGAGG CGATTCTTCC TGGAATATGA CCGACTTCAG
    GAGTTAGATC AGGGAATTGC TGGGGAGCCC AGCCAGTCTC AACAGGCTCA AGAAATGCTC
    AACAACAACA TTGAGCCTGA TCGGCCTGGT CCCTCCCATC AGCAGGCTCC ACACAGCAGT
    GAAAACAATT CCAATTTGTC CCGGGGTCAC CTGAACCGCT GTCGCGCTTG TCATAACCTG
    CTGACCTTTA ACAATGACAC GCTGCGCTGG GAAAGAAGCA CGCCTAGCTA CACATCAGGG
    CAGGTCCAAA GCACATTTGA GGGTGTGCCT CCAAATACCA GCCAGGTGCA GCCTGCAGAG
    AGAACCGAGG GCAGAGCTCC TGCCTCTAGC AGGCTGCAGC TGGGCAGCTC TTCCACCCCG
    CAGGAGGAGA GGACCGTGGG AGTGGTCTTT AACCAGGAGA CGGGGCACTG GGAGAGAGTT
    TACAGCCAGT CGGCTTCAAG CAGACCTGGG AATGTATCAC AGGAGGCCTT AAACCAGGAA
    ATGCCTGAGG AAAGCTCAGA GGAGGATTCA CTCAGGAGGA GATTGCTGGA GTCTTCCCTC
    ATTTCGTTAT CCCGCTATGA CGGAGCAGGG TCCCGGGAAC ATCCAATCTA TCCAGATCCA
    GCCAGGTTAT CTCCTGCTGC ATATTATGCT CAGAGGATGA TTCAGTATCT TTCTCGGAGG
    GACAGTATTC GACAGCGATC CATGCGATAC CAACAGAACC GTCTCCGCTC TTCCTCCTCC
    TCTTCTTCCA CTTCGGAGAA CTCAAGCCCA TCTGTAGAGG GAAATGACCT GGAATTTGAA
    GATTTTGAGG ACAATGGAGA TCGATCCAGG CACCGAGCTC CCCGTAATGC CCGCATGTCT
    GCACCATCGC TGGGGCGCTT TGTCCCAAGA CGCTTCTTGC TGCCAGAGTA CTTGCCTTAT
    GCTGGAATTT TCCACGAAAG GGGACAGCCT GGCTTGGCCA CTCATTCTTC TGTCAACAGG
    GTTTTGGCAG GTGCTGTGAT TGGAGACGGA CAGTCAGCTG TGGCCAGCAA CATTGCCAAC
    ACAACCTACC GGCTCCAGTG GTGGGACTTC ACGAAGTTTG ACCTGCCAGA AATCAGCAAT
    GCGTCCGTGA ACGTGCTTGT TCAAAACTGC AAGATTTACA ATGATGCTAG CTGTGATATA
    TCTGCAGATG GGCAGCTACT GGCAGCTTTC ATTCCCAGCA GCCAGCGAGG CTTCCCCGAT
    GAAGGAATAT TGGCTGTGTA TTCTCTGGCA CCCCATAACT TGGGCGAGAT GCTTTATACA
    AAACGATTTG GCCCTAATGC CATTTCTGTG AGCCTGTCTC CAATGGGCAG ATACGTAATG
    GTGGGACTGG CTTCCCGGCG TATCCTATTG CACCCTTCCA CAGAACATAT GGTTGCTCAA
    GTCTTCAGGC TGCAACAGCC CCATGGTGGA GAAACCTCTA TGAGGAGAGT TTTCAATGTT
    CTGTACCCAA TGCCTGCTGA CCAGAGAAGA CACGTCAGCA TAAACTCTGC TCGCTGGCTA
    CCTGAACCAG GCCTGGGATT AGCATATGGC ACCAACAAAG GGGATCTGGT GATTTGCCGA
    CCAGAGGCCT TAAACTCTGG AGTTGAATAC CACTGGGACC AGCAGAATGA GAATGTCTTC
    ACTGTGCATT CCAGCAGCAG GAGCACTGAG AGGCCTGGAA CCAGCAGAGC CACGTGGAGG
    ACAGATAGGG ACATGGGCCT GATGAATGCC ATAGGTCTAC AACCCCGAAA CCCCCCCACT
    TCTGTGACTT CACAGGGTAC CCAGACCTTG GCGCCTCAGC TGCAGAATGC CGAGACTCAG
    ACAGAGAGGG AGGTACAGGA GCAGGAATCC ACCTCTGCAG GGACTGGGGA AGGTGAAGGT
    CCAGAATATG GGGCCAGTGG GGAGGACGCC GTGAGCAGGA TACAAAGGCT GATGGCAGAA
    GGAGGCATGA CTGCCGTGGT CCAGCGGGAG CAGAGCACCA CCATGGCGTC CATGGGTGGC
    TTTGGCAACA ACATCATCGT GAGTCACCGG ATCCACCGCA GCTCTCAGAC TGGTGCGGAG
    TCCACTGGAG CTGAGGGCAC ATCAGCACAT CAGTCCACCT CGCAGCAGCT GGCAGCTGAG
    CTGGAGGGAC AGATCCTTTC AGAGTCTGTG CAGCTGTCAG AGCATGGCCT GAGCCCGCGG
    ACATCCTCTG GTGGGAGTGA AGGACGAGGC ACTGGTGATC TGGACCTGCC GGAGCAGGCC
    CGGTCCTCCA TGGACACTGA GGGACCAATC GAATACAGTG ACCTAACTAA TAATAACCAT
    CTTCCTGACA GTACCAACTT CTATAGTAAT GACAGCACCA GTGGGGAGTC GCGGAACAGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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