HECTD1 cDNA ORF clone, Gallus gallus(chicken)

  • Gene

  • Clones

  • gRNAs

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa36980 XM_015287621.2
Latest version!
Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa36981 XM_015287622.2
Latest version!
Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa36982 XM_015287623.2
Latest version!
Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa36983 XM_015287624.2
Latest version!
Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa09382 XM_004941742.3
Latest version!
Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OGa09385 XM_421227.6
Latest version!
Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OGa09385 XM_421227.5 Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OGa36983 XM_015287624.1 Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa36982 XM_015287623.1 Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa09382 XM_004941742.2 Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OGa36981 XM_015287622.1 Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa36980 XM_015287621.1 Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa36980
    Clone ID Related Accession (Same CDS sequence) XM_015287621.2 , XM_015287621.1
    Accession Version XM_015287621.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAC GTCTCCTCTT
    ATGGGTGCCC AAAGTTTTCC TAATCTGACT ACAACTGGTA CCACATCAAC AGTGACCATG
    TCTACATCCA GTGTTACTAG CAGCAGCAAT GTAGCTACAG CAACTACAGT TTTATCTGTT
    GGTCAGTCGC TTAGTAATAC TCTAACTACT AGCCTAACAT CAACATCTAG TGAGAGCGAT
    ACAGGGCAGG AGGCAGAATA TTCTTTATAT GATTTTCTGG ATAGCTGTCG AGCTAGTACT
    CTGTTGGCAG AGTTAGATGA TGATGAGGAT CTACCTGAAC CTGATGAAGA AGATGATGAA
    AATGAAGATG ATAACCAAGA AGATCAAGAA TATGAGGAAG TTATGGAAGA AGAGGAGTAT
    GAGACCAAAG GAGGTCGTAG AAGAACATGG GATGATGATT ATGTATTGAA ACGACAGTTT
    TCTGCTCTGG TTCCTGCTTT TGACCCAAGA CCTGGTCGAA CAAATGTTCA ACAAACAACT
    GATTTAGAAA TCCCTCCACC GGGTACACCT CATTCAGAAC TCTTGGAAGA GGTTGAGTGC
    ATGCCTTCAC CACGCTTAGC ACTTACGTTG AAAGTTTCAG GCCTTGGAAC AACACGAGAA
    GTAGAACTGC CCCTAACAAA TTTTCGATTC ACAATATTTT ACTACGTACA GAAATTGTTA
    CAGCTTTCCT GTAATGGCAG TGTGAAATCT GACAAACTTA GACGTATTTG GGAGCCAACA
    TACACAATCA TGTACAGAGA AATGAAGGAT TCTGATAAAG AAAAAGAAAG TGGAAAAATG
    GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG TACCTTGGCA CTGATGAATT ACCAAAGAAT
    GACTTGATAA CCTACCTGCA GAAGAATGCA GATTCAGCTT TCCTGCGCCA CTGGAAATTA
    ACCGGCACTA ATAAAAGTAT TAGGAAAAAC AGAAATTGTT CCCAGCTCAT AGCTGCATAC
    AAGGATTTTT GTGAACATGG ATCAAAATCT GGATTAAGCC AGGGGGCCAT TTCTACTCTT
    CAGAATTCTG ATATTCTTAG TTTGGCAAAA GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG
    CAGAACTCCT GTGGAGTAGA AGATGTTTTG CAGTTACTTC GCATTTTGTA TATAGTTGCT
    AGTGACCCTT ACACAGCGCG AACTTCTCAA GAAGAAGGAG ATGAGCATCC TCAGTTTAAC
    TTTCCACCAG ATGAATTCAC AAGCAAAAAA ATTACTACAA AAATTCTGCA ACAGATCGAG
    GAACCCTTGG CACTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAGCAGCT AACAAGCAAG
    TGTCCTTTCT TAATTCCATT TGAAACTAGA CAGTTATACT TCACCTGCAC AGCGTTTGGA
    GCATCAAGAG CAATTGTGTG GCTACAAAAC AGACGCGAAG CCACTGTTGA AAGAACAAGA
    ACCACAAGCA CAGTGCGACG TGATGATCCG GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT
    GAAAGGGTGA AAGTTCCGCG AGGTGAATCC CTGATGGAAT GGGCAGAAAA CGTCATGCAG
    ATTCATGCAG ACAGAAAATC TGTTCTAGAG GTTGAGTTTT TAGGGGAGGA AGGAACGGGC
    CTGGGTCCTA CCTTGGAATT TTATGCATTG GTAGCAGCAG AATTTCAGAG GACAGATCTG
    GGAGCTTGGC TTTGTGATGA TGATTTTCCA GATGATGAGT CTCGTCAGGT TGATATTGGT
    GGTGGATTAA AACCCCCTGG TTACTATGTA CAGCGATCAT GTGGACTCTT CACAGCACCA
    TTCCCACAAG ATAGTGATGA GCTTGAAAGG ATAACCAAAC TCTTTCATTT CCTTGGAATT
    TTCTTGGCTA AATGCATTCA AGACAATAGA CTTGTGGATT TACCCATTTC TAAGCCTTTT
    TTTAAACTCA TGTGTATGGG AGACATTAAA AGCAATATGA GTAAGTTGAT ATATGAATCA
    CGGAGTGACA GGGACTTGCA CTGTACTGAA AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG
    CACGATTCAT TGTCAGTAGG AAGTTTGGAA GAAGACTCCA AATCAGAATT TATTCTTGAT
    CCACCAAAAC CTAAACCTCC TGCTTGGTTT AATGGAATTC TAACGTGGGA AGACTTTGAA
    TTAATAAATC CACATAGAGC AAGGTTTCTA AAAGAAATTA AGGACCTAGC TATTAAAAGG
    CGTCAAATTT TAAGCAACAA AAGTCTGTCA GAAGATGAAA AAAATACAAA ACTACAGGAA
    TTAATGCTAA AGAATCCATC AGGTTCTGGA CCACCACTTA GCATAGAGGA TTTGGGTTTA
    AATTTTCAGT TTTGCCCTTC ATCCAAAGTG TATGGATTTA CAGCTGTAGA TCTCAAACCA
    GGTGGTGAAG ATGAGACTGT AACGATGGAT AATGCAGAGG AGTATGTGGA TCTCATGTTT
    GACTTCTGTA TGCATACTGG TATACAGAAA CAGATGGAGG CATTCAGAGA TGGCTTCAAT
    AGAGTCTTCC CAATGGAGAA GCTGAGTTCT TTCAGCCATG AAGAAGTTCA GATGATTCTT
    TGTGGAAATC AGTCACCTTC CTGGGCAGCA GAGGATATCA TCAATTATAC AGAACCTAAA
    CTGGGATATA CACGGGACAG CCCTGGATTC CTTCGATTTG TGAGAGTTCT GTGTGGTATG
    TCTTCAGATG AAAGAAAGGC TTTCCTCCAG TTCACCACTG GTTGTTCAAC ACTCCCACCA
    GGAGGACTGG CAAACCTACA TCCCCGGCTG ACTGTTGTAC GCAAGGTTGA TGCTACAGAT
    GCCAGTTACC CATCTGTGAA TACATGTGTG CATTACCTGA AGTTGCCTGA GTATTCTTCT
    GAGGAGATTA TGAGAGAGCG TCTGCTAGCT GCTACAATGG AGAAAGGCTT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143107.1
    CDS309..8051
    Translation

    Target ORF information:

    RefSeq Version XM_015287621.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287621.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAC GTCTCCTCTT
    ATGGGTGCCC AAAGTTTTCC TAATCTGACT ACAACTGGTA CCACATCAAC AGTGACCATG
    TCTACATCCA GTGTTACTAG CAGCAGCAAT GTAGCTACAG CAACTACAGT TTTATCTGTT
    GGTCAGTCGC TTAGTAATAC TCTAACTACT AGCCTAACAT CAACATCTAG TGAGAGCGAT
    ACAGGGCAGG AGGCAGAATA TTCTTTATAT GATTTTCTGG ATAGCTGTCG AGCTAGTACT
    CTGTTGGCAG AGTTAGATGA TGATGAGGAT CTACCTGAAC CTGATGAAGA AGATGATGAA
    AATGAAGATG ATAACCAAGA AGATCAAGAA TATGAGGAAG TTATGGAAGA AGAGGAGTAT
    GAGACCAAAG GAGGTCGTAG AAGAACATGG GATGATGATT ATGTATTGAA ACGACAGTTT
    TCTGCTCTGG TTCCTGCTTT TGACCCAAGA CCTGGTCGAA CAAATGTTCA ACAAACAACT
    GATTTAGAAA TCCCTCCACC GGGTACACCT CATTCAGAAC TCTTGGAAGA GGTTGAGTGC
    ATGCCTTCAC CACGCTTAGC ACTTACGTTG AAAGTTTCAG GCCTTGGAAC AACACGAGAA
    GTAGAACTGC CCCTAACAAA TTTTCGATTC ACAATATTTT ACTACGTACA GAAATTGTTA
    CAGCTTTCCT GTAATGGCAG TGTGAAATCT GACAAACTTA GACGTATTTG GGAGCCAACA
    TACACAATCA TGTACAGAGA AATGAAGGAT TCTGATAAAG AAAAAGAAAG TGGAAAAATG
    GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG TACCTTGGCA CTGATGAATT ACCAAAGAAT
    GACTTGATAA CCTACCTGCA GAAGAATGCA GATTCAGCTT TCCTGCGCCA CTGGAAATTA
    ACCGGCACTA ATAAAAGTAT TAGGAAAAAC AGAAATTGTT CCCAGCTCAT AGCTGCATAC
    AAGGATTTTT GTGAACATGG ATCAAAATCT GGATTAAGCC AGGGGGCCAT TTCTACTCTT
    CAGAATTCTG ATATTCTTAG TTTGGCAAAA GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG
    CAGAACTCCT GTGGAGTAGA AGATGTTTTG CAGTTACTTC GCATTTTGTA TATAGTTGCT
    AGTGACCCTT ACACAGCGCG AACTTCTCAA GAAGAAGGAG ATGAGCATCC TCAGTTTAAC
    TTTCCACCAG ATGAATTCAC AAGCAAAAAA ATTACTACAA AAATTCTGCA ACAGATCGAG
    GAACCCTTGG CACTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAGCAGCT AACAAGCAAG
    TGTCCTTTCT TAATTCCATT TGAAACTAGA CAGTTATACT TCACCTGCAC AGCGTTTGGA
    GCATCAAGAG CAATTGTGTG GCTACAAAAC AGACGCGAAG CCACTGTTGA AAGAACAAGA
    ACCACAAGCA CAGTGCGACG TGATGATCCG GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT
    GAAAGGGTGA AAGTTCCGCG AGGTGAATCC CTGATGGAAT GGGCAGAAAA CGTCATGCAG
    ATTCATGCAG ACAGAAAATC TGTTCTAGAG GTTGAGTTTT TAGGGGAGGA AGGAACGGGC
    CTGGGTCCTA CCTTGGAATT TTATGCATTG GTAGCAGCAG AATTTCAGAG GACAGATCTG
    GGAGCTTGGC TTTGTGATGA TGATTTTCCA GATGATGAGT CTCGTCAGGT TGATATTGGT
    GGTGGATTAA AACCCCCTGG TTACTATGTA CAGCGATCAT GTGGACTCTT CACAGCACCA
    TTCCCACAAG ATAGTGATGA GCTTGAAAGG ATAACCAAAC TCTTTCATTT CCTTGGAATT
    TTCTTGGCTA AATGCATTCA AGACAATAGA CTTGTGGATT TACCCATTTC TAAGCCTTTT
    TTTAAACTCA TGTGTATGGG AGACATTAAA AGCAATATGA GTAAGTTGAT ATATGAATCA
    CGGAGTGACA GGGACTTGCA CTGTACTGAA AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG
    CACGATTCAT TGTCAGTAGG AAGTTTGGAA GAAGACTCCA AATCAGAATT TATTCTTGAT
    CCACCAAAAC CTAAACCTCC TGCTTGGTTT AATGGAATTC TAACGTGGGA AGACTTTGAA
    TTAATAAATC CACATAGAGC AAGGTTTCTA AAAGAAATTA AGGACCTAGC TATTAAAAGG
    CGTCAAATTT TAAGCAACAA AAGTCTGTCA GAAGATGAAA AAAATACAAA ACTACAGGAA
    TTAATGCTAA AGAATCCATC AGGTTCTGGA CCACCACTTA GCATAGAGGA TTTGGGTTTA
    AATTTTCAGT TTTGCCCTTC ATCCAAAGTG TATGGATTTA CAGCTGTAGA TCTCAAACCA
    GGTGGTGAAG ATGAGACTGT AACGATGGAT AATGCAGAGG AGTATGTGGA TCTCATGTTT
    GACTTCTGTA TGCATACTGG TATACAGAAA CAGATGGAGG CATTCAGAGA TGGCTTCAAT
    AGAGTCTTCC CAATGGAGAA GCTGAGTTCT TTCAGCCATG AAGAAGTTCA GATGATTCTT
    TGTGGAAATC AGTCACCTTC CTGGGCAGCA GAGGATATCA TCAATTATAC AGAACCTAAA
    CTGGGATATA CACGGGACAG CCCTGGATTC CTTCGATTTG TGAGAGTTCT GTGTGGTATG
    TCTTCAGATG AAAGAAAGGC TTTCCTCCAG TTCACCACTG GTTGTTCAAC ACTCCCACCA
    GGAGGACTGG CAAACCTACA TCCCCGGCTG ACTGTTGTAC GCAAGGTTGA TGCTACAGAT
    GCCAGTTACC CATCTGTGAA TACATGTGTG CATTACCTGA AGTTGCCTGA GTATTCTTCT
    GAGGAGATTA TGAGAGAGCG TCTGCTAGCT GCTACAATGG AGAAAGGCTT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36980
    Clone ID Related Accession (Same CDS sequence) XM_015287621.2 , XM_015287621.1
    Accession Version XM_015287621.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAC GTCTCCTCTT
    ATGGGTGCCC AAAGTTTTCC TAATCTGACT ACAACTGGTA CCACATCAAC AGTGACCATG
    TCTACATCCA GTGTTACTAG CAGCAGCAAT GTAGCTACAG CAACTACAGT TTTATCTGTT
    GGTCAGTCGC TTAGTAATAC TCTAACTACT AGCCTAACAT CAACATCTAG TGAGAGCGAT
    ACAGGGCAGG AGGCAGAATA TTCTTTATAT GATTTTCTGG ATAGCTGTCG AGCTAGTACT
    CTGTTGGCAG AGTTAGATGA TGATGAGGAT CTACCTGAAC CTGATGAAGA AGATGATGAA
    AATGAAGATG ATAACCAAGA AGATCAAGAA TATGAGGAAG TTATGGAAGA AGAGGAGTAT
    GAGACCAAAG GAGGTCGTAG AAGAACATGG GATGATGATT ATGTATTGAA ACGACAGTTT
    TCTGCTCTGG TTCCTGCTTT TGACCCAAGA CCTGGTCGAA CAAATGTTCA ACAAACAACT
    GATTTAGAAA TCCCTCCACC GGGTACACCT CATTCAGAAC TCTTGGAAGA GGTTGAGTGC
    ATGCCTTCAC CACGCTTAGC ACTTACGTTG AAAGTTTCAG GCCTTGGAAC AACACGAGAA
    GTAGAACTGC CCCTAACAAA TTTTCGATTC ACAATATTTT ACTACGTACA GAAATTGTTA
    CAGCTTTCCT GTAATGGCAG TGTGAAATCT GACAAACTTA GACGTATTTG GGAGCCAACA
    TACACAATCA TGTACAGAGA AATGAAGGAT TCTGATAAAG AAAAAGAAAG TGGAAAAATG
    GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG TACCTTGGCA CTGATGAATT ACCAAAGAAT
    GACTTGATAA CCTACCTGCA GAAGAATGCA GATTCAGCTT TCCTGCGCCA CTGGAAATTA
    ACCGGCACTA ATAAAAGTAT TAGGAAAAAC AGAAATTGTT CCCAGCTCAT AGCTGCATAC
    AAGGATTTTT GTGAACATGG ATCAAAATCT GGATTAAGCC AGGGGGCCAT TTCTACTCTT
    CAGAATTCTG ATATTCTTAG TTTGGCAAAA GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG
    CAGAACTCCT GTGGAGTAGA AGATGTTTTG CAGTTACTTC GCATTTTGTA TATAGTTGCT
    AGTGACCCTT ACACAGCGCG AACTTCTCAA GAAGAAGGAG ATGAGCATCC TCAGTTTAAC
    TTTCCACCAG ATGAATTCAC AAGCAAAAAA ATTACTACAA AAATTCTGCA ACAGATCGAG
    GAACCCTTGG CACTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAGCAGCT AACAAGCAAG
    TGTCCTTTCT TAATTCCATT TGAAACTAGA CAGTTATACT TCACCTGCAC AGCGTTTGGA
    GCATCAAGAG CAATTGTGTG GCTACAAAAC AGACGCGAAG CCACTGTTGA AAGAACAAGA
    ACCACAAGCA CAGTGCGACG TGATGATCCG GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT
    GAAAGGGTGA AAGTTCCGCG AGGTGAATCC CTGATGGAAT GGGCAGAAAA CGTCATGCAG
    ATTCATGCAG ACAGAAAATC TGTTCTAGAG GTTGAGTTTT TAGGGGAGGA AGGAACGGGC
    CTGGGTCCTA CCTTGGAATT TTATGCATTG GTAGCAGCAG AATTTCAGAG GACAGATCTG
    GGAGCTTGGC TTTGTGATGA TGATTTTCCA GATGATGAGT CTCGTCAGGT TGATATTGGT
    GGTGGATTAA AACCCCCTGG TTACTATGTA CAGCGATCAT GTGGACTCTT CACAGCACCA
    TTCCCACAAG ATAGTGATGA GCTTGAAAGG ATAACCAAAC TCTTTCATTT CCTTGGAATT
    TTCTTGGCTA AATGCATTCA AGACAATAGA CTTGTGGATT TACCCATTTC TAAGCCTTTT
    TTTAAACTCA TGTGTATGGG AGACATTAAA AGCAATATGA GTAAGTTGAT ATATGAATCA
    CGGAGTGACA GGGACTTGCA CTGTACTGAA AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG
    CACGATTCAT TGTCAGTAGG AAGTTTGGAA GAAGACTCCA AATCAGAATT TATTCTTGAT
    CCACCAAAAC CTAAACCTCC TGCTTGGTTT AATGGAATTC TAACGTGGGA AGACTTTGAA
    TTAATAAATC CACATAGAGC AAGGTTTCTA AAAGAAATTA AGGACCTAGC TATTAAAAGG
    CGTCAAATTT TAAGCAACAA AAGTCTGTCA GAAGATGAAA AAAATACAAA ACTACAGGAA
    TTAATGCTAA AGAATCCATC AGGTTCTGGA CCACCACTTA GCATAGAGGA TTTGGGTTTA
    AATTTTCAGT TTTGCCCTTC ATCCAAAGTG TATGGATTTA CAGCTGTAGA TCTCAAACCA
    GGTGGTGAAG ATGAGACTGT AACGATGGAT AATGCAGAGG AGTATGTGGA TCTCATGTTT
    GACTTCTGTA TGCATACTGG TATACAGAAA CAGATGGAGG CATTCAGAGA TGGCTTCAAT
    AGAGTCTTCC CAATGGAGAA GCTGAGTTCT TTCAGCCATG AAGAAGTTCA GATGATTCTT
    TGTGGAAATC AGTCACCTTC CTGGGCAGCA GAGGATATCA TCAATTATAC AGAACCTAAA
    CTGGGATATA CACGGGACAG CCCTGGATTC CTTCGATTTG TGAGAGTTCT GTGTGGTATG
    TCTTCAGATG AAAGAAAGGC TTTCCTCCAG TTCACCACTG GTTGTTCAAC ACTCCCACCA
    GGAGGACTGG CAAACCTACA TCCCCGGCTG ACTGTTGTAC GCAAGGTTGA TGCTACAGAT
    GCCAGTTACC CATCTGTGAA TACATGTGTG CATTACCTGA AGTTGCCTGA GTATTCTTCT
    GAGGAGATTA TGAGAGAGCG TCTGCTAGCT GCTACAATGG AGAAAGGCTT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143107.1
    CDS286..8028
    Translation

    Target ORF information:

    RefSeq Version XM_015287621.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAC GTCTCCTCTT
    ATGGGTGCCC AAAGTTTTCC TAATCTGACT ACAACTGGTA CCACATCAAC AGTGACCATG
    TCTACATCCA GTGTTACTAG CAGCAGCAAT GTAGCTACAG CAACTACAGT TTTATCTGTT
    GGTCAGTCGC TTAGTAATAC TCTAACTACT AGCCTAACAT CAACATCTAG TGAGAGCGAT
    ACAGGGCAGG AGGCAGAATA TTCTTTATAT GATTTTCTGG ATAGCTGTCG AGCTAGTACT
    CTGTTGGCAG AGTTAGATGA TGATGAGGAT CTACCTGAAC CTGATGAAGA AGATGATGAA
    AATGAAGATG ATAACCAAGA AGATCAAGAA TATGAGGAAG TTATGGAAGA AGAGGAGTAT
    GAGACCAAAG GAGGTCGTAG AAGAACATGG GATGATGATT ATGTATTGAA ACGACAGTTT
    TCTGCTCTGG TTCCTGCTTT TGACCCAAGA CCTGGTCGAA CAAATGTTCA ACAAACAACT
    GATTTAGAAA TCCCTCCACC GGGTACACCT CATTCAGAAC TCTTGGAAGA GGTTGAGTGC
    ATGCCTTCAC CACGCTTAGC ACTTACGTTG AAAGTTTCAG GCCTTGGAAC AACACGAGAA
    GTAGAACTGC CCCTAACAAA TTTTCGATTC ACAATATTTT ACTACGTACA GAAATTGTTA
    CAGCTTTCCT GTAATGGCAG TGTGAAATCT GACAAACTTA GACGTATTTG GGAGCCAACA
    TACACAATCA TGTACAGAGA AATGAAGGAT TCTGATAAAG AAAAAGAAAG TGGAAAAATG
    GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG TACCTTGGCA CTGATGAATT ACCAAAGAAT
    GACTTGATAA CCTACCTGCA GAAGAATGCA GATTCAGCTT TCCTGCGCCA CTGGAAATTA
    ACCGGCACTA ATAAAAGTAT TAGGAAAAAC AGAAATTGTT CCCAGCTCAT AGCTGCATAC
    AAGGATTTTT GTGAACATGG ATCAAAATCT GGATTAAGCC AGGGGGCCAT TTCTACTCTT
    CAGAATTCTG ATATTCTTAG TTTGGCAAAA GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG
    CAGAACTCCT GTGGAGTAGA AGATGTTTTG CAGTTACTTC GCATTTTGTA TATAGTTGCT
    AGTGACCCTT ACACAGCGCG AACTTCTCAA GAAGAAGGAG ATGAGCATCC TCAGTTTAAC
    TTTCCACCAG ATGAATTCAC AAGCAAAAAA ATTACTACAA AAATTCTGCA ACAGATCGAG
    GAACCCTTGG CACTAGCAAG TGGAGCTTTG CCAGACTGGT GTGAGCAGCT AACAAGCAAG
    TGTCCTTTCT TAATTCCATT TGAAACTAGA CAGTTATACT TCACCTGCAC AGCGTTTGGA
    GCATCAAGAG CAATTGTGTG GCTACAAAAC AGACGCGAAG CCACTGTTGA AAGAACAAGA
    ACCACAAGCA CAGTGCGACG TGATGATCCG GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT
    GAAAGGGTGA AAGTTCCGCG AGGTGAATCC CTGATGGAAT GGGCAGAAAA CGTCATGCAG
    ATTCATGCAG ACAGAAAATC TGTTCTAGAG GTTGAGTTTT TAGGGGAGGA AGGAACGGGC
    CTGGGTCCTA CCTTGGAATT TTATGCATTG GTAGCAGCAG AATTTCAGAG GACAGATCTG
    GGAGCTTGGC TTTGTGATGA TGATTTTCCA GATGATGAGT CTCGTCAGGT TGATATTGGT
    GGTGGATTAA AACCCCCTGG TTACTATGTA CAGCGATCAT GTGGACTCTT CACAGCACCA
    TTCCCACAAG ATAGTGATGA GCTTGAAAGG ATAACCAAAC TCTTTCATTT CCTTGGAATT
    TTCTTGGCTA AATGCATTCA AGACAATAGA CTTGTGGATT TACCCATTTC TAAGCCTTTT
    TTTAAACTCA TGTGTATGGG AGACATTAAA AGCAATATGA GTAAGTTGAT ATATGAATCA
    CGGAGTGACA GGGACTTGCA CTGTACTGAA AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG
    CACGATTCAT TGTCAGTAGG AAGTTTGGAA GAAGACTCCA AATCAGAATT TATTCTTGAT
    CCACCAAAAC CTAAACCTCC TGCTTGGTTT AATGGAATTC TAACGTGGGA AGACTTTGAA
    TTAATAAATC CACATAGAGC AAGGTTTCTA AAAGAAATTA AGGACCTAGC TATTAAAAGG
    CGTCAAATTT TAAGCAACAA AAGTCTGTCA GAAGATGAAA AAAATACAAA ACTACAGGAA
    TTAATGCTAA AGAATCCATC AGGTTCTGGA CCACCACTTA GCATAGAGGA TTTGGGTTTA
    AATTTTCAGT TTTGCCCTTC ATCCAAAGTG TATGGATTTA CAGCTGTAGA TCTCAAACCA
    GGTGGTGAAG ATGAGACTGT AACGATGGAT AATGCAGAGG AGTATGTGGA TCTCATGTTT
    GACTTCTGTA TGCATACTGG TATACAGAAA CAGATGGAGG CATTCAGAGA TGGCTTCAAT
    AGAGTCTTCC CAATGGAGAA GCTGAGTTCT TTCAGCCATG AAGAAGTTCA GATGATTCTT
    TGTGGAAATC AGTCACCTTC CTGGGCAGCA GAGGATATCA TCAATTATAC AGAACCTAAA
    CTGGGATATA CACGGGACAG CCCTGGATTC CTTCGATTTG TGAGAGTTCT GTGTGGTATG
    TCTTCAGATG AAAGAAAGGC TTTCCTCCAG TTCACCACTG GTTGTTCAAC ACTCCCACCA
    GGAGGACTGG CAAACCTACA TCCCCGGCTG ACTGTTGTAC GCAAGGTTGA TGCTACAGAT
    GCCAGTTACC CATCTGTGAA TACATGTGTG CATTACCTGA AGTTGCCTGA GTATTCTTCT
    GAGGAGATTA TGAGAGAGCG TCTGCTAGCT GCTACAATGG AGAAAGGCTT CCATCTCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36981
    Clone ID Related Accession (Same CDS sequence) XM_015287622.1 , XM_015287622.2
    Accession Version XM_015287622.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG TAAGTATGTA GGATCAGAAT TTGTGACTGG TTGGACTGGC
    AAAAGAGGAA GGAAGCTAAA ATCTAAACTG GAGAAGACAA AGCAGAAGGT ACGCACTATG
    GCAAGAGATT TATACGATGA TCATTTTAAA GCTGTTGAAA GCATGCCTCG AGGAGTAGTT
    GTAACACTGA GGAACATAGC AACACAGTTA GAGTCTGCGT GGGAACTTCA TACAAACAGA
    CAGTGTATTG AGGGAGAAAA CACTTGGAGA GATTTAATGA AGACTGCTTT GGAAAATTTA
    ATTGTGCTTT TGAAGGATGA GAATACCATT TCTCCATACG AAATGTGCAG TAGTGGATTA
    GTTCAATCAT TGCTTACAGT TTTAAATAAC AATGTTGATT TAGATATGAA ACAAGACTGT
    AGTCAACTAG TTGAAAGAAT AAATGTATTC AAAACAGCAT TCAGCGAAAA TGAAGATGAT
    GAAAGTCGAC CAGCAGTTGC ATTAATTCGG AAATTAATAG CAGTGTTAGA ATCTATTGAA
    CGTCTACCTC TTCACTTGTA TGATACACCA GGATCTACTT ATAATCTTCA GATATTGACA
    AGACGTTTGA GGTTTCGTTT GGAACGTGCT TCAGGGGAGA CATCCTTGAT AGATCGAACT
    GGCAGAATGC TAAAGATGGA GCCATTGGCA ACTGTTGAAT CTCTAGAACA GTATCTTCTG
    AAAATGGTTG CAAAGCAATG GTACGACTTT GATAGGGCTT CATTTGTTTT TGTACGAAAA
    CTGCGTGAAG GCCAAACATT TGTATTCAGG CATCAACATG ATTTTGATGA AAATGGAATT
    ATTTACTGGA TTGGGACAAA TGCGAAAACT GCATATGAGT GGGTAAATCC AGCAGCCTAT
    GGGTTAGTAG TGGTTACATC CTCAGAAGGA AGAAATCTGC CTTATGGCCG TTTAGAAGAC
    ATCCTAAGCC GTGACAGTTC AGCTCTCAAT TGTCATACTA ATGATGATAA GAATGCTTGG
    TTTGCCATAG ATCTTGGCCT TTGGGTTATA CCATCAGCTT ACACGTTACG CCATGCTCGA
    GGTTATGGAC GATCTGCTCT CAGAAATTGG GTTTTCCAGG TATCTAAGGA TGGACAGAAC
    TGGACTACTT TGTATACTCA TGTTGATGAT TGTAGTCTCA ACGAACCAGG GTCTACAGCA
    ACATGGCCTT TAGATCCTCC AAAGGATGAG AAACAAGGTT GGAGGCATGT TAGAATAAAA
    CAGATGGGAA AGAATGCCAG TGGGCAAACA CACTACCTCT CTCTGTCTGG ATTTGAACTT
    TATGGCACTG TGAATGGAGT ATGTGAAGAC CAGTTGGGAA AAGCTGCTAA AGAAGCTGAA
    GCAAATCTGC GAAGGCAGCG ACGTCTGGTC CGGTCTCAAG TTTTGAAATA CATGGTTCCA
    GGAGCTCGGG TAATCAGAGG AATTGATTGG AAGTGGAGAG ATCAGGATGG AAGCCCACAG
    GGTGAAGGCA CTGTTACAGG AGAATTGCAC AATGGCTGGA TTGATGTCAC CTGGGATGCT
    GGTGGCTCTA ACTCTTACCG TATGGGCGCA GAAGGAAAGT TTGACCTCAA GCTTGCACCA
    GGGTACGACC CTGATTCAGC GGCATCACCC AAACCTGTTT CATCCACTGT TTCAGGCACA
    ACGCAGTCAT GGAGCAGCTT GGTGAAAAAC AACTGTCCAG ACAAGACGAC TGCTGCTGCA
    GGCTCCTCAA GTAGAAAAGG AAGCAGCAGT TCTGTGTGTA GCGTGGCCAG TAGCAGCGAC
    ATCAGCTTGG GTTCGACCAA AATGGAGCGG AGATCAGAAA GTGTAATGGA GCAAAATATA
    GTTTCAGGCA CTGACGTCCA CGAACCAATT GTTGTTCTGT CTTCCGCTGA TAATGTACCT
    CAGGCTGAAG TAGGGTCATC TTCCAGTGCA AGCACCAGCA CCTTAACAGC GGACATGGGA
    AATGAAAATA CAGAAAGGAA GTTAGGTCCT GATAATTCGA TACGTACTCC TGGGGAGTCC
    AGTGCTATCT CCATGGGAAT TGTTAGTGTA AGTTCTCCTG ATGTTAGTTC AGTTTCCGAA
    CTGACTAATA AAGAAGCAGC ATCCCAACGA CCTCTGAGCT CTTCAGCAAG TAACAGACTT
    TCAGTTAGCT CTTTGTTGGC TGCTGGGGCG CCAATGAGCT CCAGTGCAAG CGTTCCTAAC
    TTATCATCTC GAGAAACTTC GAGCCTGGAG TCTTTTGTAC GAAGAGTGGC AAACATAGCA
    CGTACCAATG CCACAAACAA CATGAATTTA AGCCGAAGTA GCAGTGATAA CAATACTAAT
    ACTCTGGGAA GAAACGTCAT GAGTACTGCA ACGTCTCCTC TTATGGGTGC CCAAAGTTTT
    CCTAATCTGA CTACAACTGG TACCACATCA ACAGTGACCA TGTCTACATC CAGTGTTACT
    AGCAGCAGCA ATGTAGCTAC AGCAACTACA GTTTTATCTG TTGGTCAGTC GCTTAGTAAT
    ACTCTAACTA CTAGCCTAAC ATCAACATCT AGTGAGAGCG ATACAGGGCA GGAGGCAGAA
    TATTCTTTAT ATGATTTTCT GGATAGCTGT CGAGCTAGTA CTCTGTTGGC AGAGTTAGAT
    GATGATGAGG ATCTACCTGA ACCTGATGAA GAAGATGATG AAAATGAAGA TGATAACCAA
    GAAGATCAAG AATATGAGGA AGTTATGGAA GAAGAGGAGT ATGAGACCAA AGGAGGTCGT
    AGAAGAACAT GGGATGATGA TTATGTATTG AAACGACAGT TTTCTGCTCT GGTTCCTGCT
    TTTGACCCAA GACCTGGTCG AACAAATGTT CAACAAACAA CTGATTTAGA AATCCCTCCA
    CCGGGTACAC CTCATTCAGA ACTCTTGGAA GAGGTTGAGT GCATGCCTTC ACCACGCTTA
    GCACTTACGT TGAAAGTTTC AGGCCTTGGA ACAACACGAG AAGTAGAACT GCCCCTAACA
    AATTTTCGAT TCACAATATT TTACTACGTA CAGAAATTGT TACAGCTTTC CTGTAATGGC
    AGTGTGAAAT CTGACAAACT TAGACGTATT TGGGAGCCAA CATACACAAT CATGTACAGA
    GAAATGAAGG ATTCTGATAA AGAAAAAGAA AGTGGAAAAA TGGGTTGCTG GTCAGTAGAG
    CATGTGGAGC AGTACCTTGG CACTGATGAA TTACCAAAGA ATGACTTGAT AACCTACCTG
    CAGAAGAATG CAGATTCAGC TTTCCTGCGC CACTGGAAAT TAACCGGCAC TAATAAAAGT
    ATTAGGAAAA ACAGAAATTG TTCCCAGCTC ATAGCTGCAT ACAAGGATTT TTGTGAACAT
    GGATCAAAAT CTGGATTAAG CCAGGGGGCC ATTTCTACTC TTCAGAATTC TGATATTCTT
    AGTTTGGCAA AAGAGCAGCC TCAAGCCAAA GCTGGCAGTG GGCAGAACTC CTGTGGAGTA
    GAAGATGTTT TGCAGTTACT TCGCATTTTG TATATAGTTG CTAGTGACCC TTACACAGCG
    CGAACTTCTC AAGAAGAAGG AGATGAGCAT CCTCAGTTTA ACTTTCCACC AGATGAATTC
    ACAAGCAAAA AAATTACTAC AAAAATTCTG CAACAGATCG AGGAACCCTT GGCACTAGCA
    AGTGGAGCTT TGCCAGACTG GTGTGAGCAG CTAACAAGCA AGTGTCCTTT CTTAATTCCA
    TTTGAAACTA GACAGTTATA CTTCACCTGC ACAGCGTTTG GAGCATCAAG AGCAATTGTG
    TGGCTACAAA ACAGACGCGA AGCCACTGTT GAAAGAACAA GAACCACAAG CACAGTGCGA
    CGTGATGATC CGGGAGAGTT CAGAGTGGGG CGCCTGAAGC ATGAAAGGGT GAAAGTTCCG
    CGAGGTGAAT CCCTGATGGA ATGGGCAGAA AACGTCATGC AGATTCATGC AGACAGAAAA
    TCTGTTCTAG AGGTTGAGTT TTTAGGGGAG GAAGGAACGG GCCTGGGTCC TACCTTGGAA
    TTTTATGCAT TGGTAGCAGC AGAATTTCAG AGGACAGATC TGGGAGCTTG GCTTTGTGAT
    GATGATTTTC CAGATGATGA GTCTCGTCAG GTTGATATTG GTGGTGGATT AAAACCCCCT
    GGTTACTATG TACAGCGATC ATGTGGACTC TTCACAGCAC CATTCCCACA AGATAGTGAT
    GAGCTTGAAA GGATAACCAA ACTCTTTCAT TTCCTTGGAA TTTTCTTGGC TAAATGCATT
    CAAGACAATA GACTTGTGGA TTTACCCATT TCTAAGCCTT TTTTTAAACT CATGTGTATG
    GGAGACATTA AAAGCAATAT GAGTAAGTTG ATATATGAAT CACGGAGTGA CAGGGACTTG
    CACTGTACTG AAAGTCAGTC AGAGGCTTCT ACAGAAGAAG GGCACGATTC ATTGTCAGTA
    GGAAGTTTGG AAGAAGACTC CAAATCAGAA TTTATTCTTG ATCCACCAAA ACCTAAACCT
    CCTGCTTGGT TTAATGGAAT TCTAACGTGG GAAGACTTTG AATTAATAAA TCCACATAGA
    GCAAGGTTTC TAAAAGAAAT TAAGGACCTA GCTATTAAAA GGCGTCAAAT TTTAAGCAAC
    AAAAGTCTGT CAGAAGATGA AAAAAATACA AAACTACAGG AATTAATGCT AAAGAATCCA
    TCAGGTTCTG GACCACCACT TAGCATAGAG GATTTGGGTT TAAATTTTCA GTTTTGCCCT
    TCATCCAAAG TGTATGGATT TACAGCTGTA GATCTCAAAC CAGGTGGTGA AGATGAGACT
    GTAACGATGG ATAATGCAGA GGAGTATGTG GATCTCATGT TTGACTTCTG TATGCATACT
    GGTATACAGA AACAGATGGA GGCATTCAGA GATGGCTTCA ATAGAGTCTT CCCAATGGAG
    AAGCTGAGTT CTTTCAGCCA TGAAGAAGTT CAGATGATTC TTTGTGGAAA TCAGTCACCT
    TCCTGGGCAG CAGAGGATAT CATCAATTAT ACAGAACCTA AACTGGGATA TACACGGGAC
    AGCCCTGGAT TCCTTCGATT TGTGAGAGTT CTGTGTGGTA TGTCTTCAGA TGAAAGAAAG
    GCTTTCCTCC AGTTCACCAC TGGTTGTTCA ACACTCCCAC CAGGAGGACT GGCAAACCTA
    CATCCCCGGC TGACTGTTGT ACGCAAGGTT GATGCTACAG ATGCCAGTTA CCCATCTGTG
    AATACATGTG TGCATTACCT GAAGTTGCCT GAGTATTCTT CTGAGGAGAT TATGAGAGAG
    CGTCTGCTAG CTGCTACAAT GGAGAAAGGC TTCCATCTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143108.1
    CDS286..8010
    Translation

    Target ORF information:

    RefSeq Version XM_015287622.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG TAAGTATGTA GGATCAGAAT TTGTGACTGG TTGGACTGGC
    AAAAGAGGAA GGAAGCTAAA ATCTAAACTG GAGAAGACAA AGCAGAAGGT ACGCACTATG
    GCAAGAGATT TATACGATGA TCATTTTAAA GCTGTTGAAA GCATGCCTCG AGGAGTAGTT
    GTAACACTGA GGAACATAGC AACACAGTTA GAGTCTGCGT GGGAACTTCA TACAAACAGA
    CAGTGTATTG AGGGAGAAAA CACTTGGAGA GATTTAATGA AGACTGCTTT GGAAAATTTA
    ATTGTGCTTT TGAAGGATGA GAATACCATT TCTCCATACG AAATGTGCAG TAGTGGATTA
    GTTCAATCAT TGCTTACAGT TTTAAATAAC AATGTTGATT TAGATATGAA ACAAGACTGT
    AGTCAACTAG TTGAAAGAAT AAATGTATTC AAAACAGCAT TCAGCGAAAA TGAAGATGAT
    GAAAGTCGAC CAGCAGTTGC ATTAATTCGG AAATTAATAG CAGTGTTAGA ATCTATTGAA
    CGTCTACCTC TTCACTTGTA TGATACACCA GGATCTACTT ATAATCTTCA GATATTGACA
    AGACGTTTGA GGTTTCGTTT GGAACGTGCT TCAGGGGAGA CATCCTTGAT AGATCGAACT
    GGCAGAATGC TAAAGATGGA GCCATTGGCA ACTGTTGAAT CTCTAGAACA GTATCTTCTG
    AAAATGGTTG CAAAGCAATG GTACGACTTT GATAGGGCTT CATTTGTTTT TGTACGAAAA
    CTGCGTGAAG GCCAAACATT TGTATTCAGG CATCAACATG ATTTTGATGA AAATGGAATT
    ATTTACTGGA TTGGGACAAA TGCGAAAACT GCATATGAGT GGGTAAATCC AGCAGCCTAT
    GGGTTAGTAG TGGTTACATC CTCAGAAGGA AGAAATCTGC CTTATGGCCG TTTAGAAGAC
    ATCCTAAGCC GTGACAGTTC AGCTCTCAAT TGTCATACTA ATGATGATAA GAATGCTTGG
    TTTGCCATAG ATCTTGGCCT TTGGGTTATA CCATCAGCTT ACACGTTACG CCATGCTCGA
    GGTTATGGAC GATCTGCTCT CAGAAATTGG GTTTTCCAGG TATCTAAGGA TGGACAGAAC
    TGGACTACTT TGTATACTCA TGTTGATGAT TGTAGTCTCA ACGAACCAGG GTCTACAGCA
    ACATGGCCTT TAGATCCTCC AAAGGATGAG AAACAAGGTT GGAGGCATGT TAGAATAAAA
    CAGATGGGAA AGAATGCCAG TGGGCAAACA CACTACCTCT CTCTGTCTGG ATTTGAACTT
    TATGGCACTG TGAATGGAGT ATGTGAAGAC CAGTTGGGAA AAGCTGCTAA AGAAGCTGAA
    GCAAATCTGC GAAGGCAGCG ACGTCTGGTC CGGTCTCAAG TTTTGAAATA CATGGTTCCA
    GGAGCTCGGG TAATCAGAGG AATTGATTGG AAGTGGAGAG ATCAGGATGG AAGCCCACAG
    GGTGAAGGCA CTGTTACAGG AGAATTGCAC AATGGCTGGA TTGATGTCAC CTGGGATGCT
    GGTGGCTCTA ACTCTTACCG TATGGGCGCA GAAGGAAAGT TTGACCTCAA GCTTGCACCA
    GGGTACGACC CTGATTCAGC GGCATCACCC AAACCTGTTT CATCCACTGT TTCAGGCACA
    ACGCAGTCAT GGAGCAGCTT GGTGAAAAAC AACTGTCCAG ACAAGACGAC TGCTGCTGCA
    GGCTCCTCAA GTAGAAAAGG AAGCAGCAGT TCTGTGTGTA GCGTGGCCAG TAGCAGCGAC
    ATCAGCTTGG GTTCGACCAA AATGGAGCGG AGATCAGAAA GTGTAATGGA GCAAAATATA
    GTTTCAGGCA CTGACGTCCA CGAACCAATT GTTGTTCTGT CTTCCGCTGA TAATGTACCT
    CAGGCTGAAG TAGGGTCATC TTCCAGTGCA AGCACCAGCA CCTTAACAGC GGACATGGGA
    AATGAAAATA CAGAAAGGAA GTTAGGTCCT GATAATTCGA TACGTACTCC TGGGGAGTCC
    AGTGCTATCT CCATGGGAAT TGTTAGTGTA AGTTCTCCTG ATGTTAGTTC AGTTTCCGAA
    CTGACTAATA AAGAAGCAGC ATCCCAACGA CCTCTGAGCT CTTCAGCAAG TAACAGACTT
    TCAGTTAGCT CTTTGTTGGC TGCTGGGGCG CCAATGAGCT CCAGTGCAAG CGTTCCTAAC
    TTATCATCTC GAGAAACTTC GAGCCTGGAG TCTTTTGTAC GAAGAGTGGC AAACATAGCA
    CGTACCAATG CCACAAACAA CATGAATTTA AGCCGAAGTA GCAGTGATAA CAATACTAAT
    ACTCTGGGAA GAAACGTCAT GAGTACTGCA ACGTCTCCTC TTATGGGTGC CCAAAGTTTT
    CCTAATCTGA CTACAACTGG TACCACATCA ACAGTGACCA TGTCTACATC CAGTGTTACT
    AGCAGCAGCA ATGTAGCTAC AGCAACTACA GTTTTATCTG TTGGTCAGTC GCTTAGTAAT
    ACTCTAACTA CTAGCCTAAC ATCAACATCT AGTGAGAGCG ATACAGGGCA GGAGGCAGAA
    TATTCTTTAT ATGATTTTCT GGATAGCTGT CGAGCTAGTA CTCTGTTGGC AGAGTTAGAT
    GATGATGAGG ATCTACCTGA ACCTGATGAA GAAGATGATG AAAATGAAGA TGATAACCAA
    GAAGATCAAG AATATGAGGA AGTTATGGAA GAAGAGGAGT ATGAGACCAA AGGAGGTCGT
    AGAAGAACAT GGGATGATGA TTATGTATTG AAACGACAGT TTTCTGCTCT GGTTCCTGCT
    TTTGACCCAA GACCTGGTCG AACAAATGTT CAACAAACAA CTGATTTAGA AATCCCTCCA
    CCGGGTACAC CTCATTCAGA ACTCTTGGAA GAGGTTGAGT GCATGCCTTC ACCACGCTTA
    GCACTTACGT TGAAAGTTTC AGGCCTTGGA ACAACACGAG AAGTAGAACT GCCCCTAACA
    AATTTTCGAT TCACAATATT TTACTACGTA CAGAAATTGT TACAGCTTTC CTGTAATGGC
    AGTGTGAAAT CTGACAAACT TAGACGTATT TGGGAGCCAA CATACACAAT CATGTACAGA
    GAAATGAAGG ATTCTGATAA AGAAAAAGAA AGTGGAAAAA TGGGTTGCTG GTCAGTAGAG
    CATGTGGAGC AGTACCTTGG CACTGATGAA TTACCAAAGA ATGACTTGAT AACCTACCTG
    CAGAAGAATG CAGATTCAGC TTTCCTGCGC CACTGGAAAT TAACCGGCAC TAATAAAAGT
    ATTAGGAAAA ACAGAAATTG TTCCCAGCTC ATAGCTGCAT ACAAGGATTT TTGTGAACAT
    GGATCAAAAT CTGGATTAAG CCAGGGGGCC ATTTCTACTC TTCAGAATTC TGATATTCTT
    AGTTTGGCAA AAGAGCAGCC TCAAGCCAAA GCTGGCAGTG GGCAGAACTC CTGTGGAGTA
    GAAGATGTTT TGCAGTTACT TCGCATTTTG TATATAGTTG CTAGTGACCC TTACACAGCG
    CGAACTTCTC AAGAAGAAGG AGATGAGCAT CCTCAGTTTA ACTTTCCACC AGATGAATTC
    ACAAGCAAAA AAATTACTAC AAAAATTCTG CAACAGATCG AGGAACCCTT GGCACTAGCA
    AGTGGAGCTT TGCCAGACTG GTGTGAGCAG CTAACAAGCA AGTGTCCTTT CTTAATTCCA
    TTTGAAACTA GACAGTTATA CTTCACCTGC ACAGCGTTTG GAGCATCAAG AGCAATTGTG
    TGGCTACAAA ACAGACGCGA AGCCACTGTT GAAAGAACAA GAACCACAAG CACAGTGCGA
    CGTGATGATC CGGGAGAGTT CAGAGTGGGG CGCCTGAAGC ATGAAAGGGT GAAAGTTCCG
    CGAGGTGAAT CCCTGATGGA ATGGGCAGAA AACGTCATGC AGATTCATGC AGACAGAAAA
    TCTGTTCTAG AGGTTGAGTT TTTAGGGGAG GAAGGAACGG GCCTGGGTCC TACCTTGGAA
    TTTTATGCAT TGGTAGCAGC AGAATTTCAG AGGACAGATC TGGGAGCTTG GCTTTGTGAT
    GATGATTTTC CAGATGATGA GTCTCGTCAG GTTGATATTG GTGGTGGATT AAAACCCCCT
    GGTTACTATG TACAGCGATC ATGTGGACTC TTCACAGCAC CATTCCCACA AGATAGTGAT
    GAGCTTGAAA GGATAACCAA ACTCTTTCAT TTCCTTGGAA TTTTCTTGGC TAAATGCATT
    CAAGACAATA GACTTGTGGA TTTACCCATT TCTAAGCCTT TTTTTAAACT CATGTGTATG
    GGAGACATTA AAAGCAATAT GAGTAAGTTG ATATATGAAT CACGGAGTGA CAGGGACTTG
    CACTGTACTG AAAGTCAGTC AGAGGCTTCT ACAGAAGAAG GGCACGATTC ATTGTCAGTA
    GGAAGTTTGG AAGAAGACTC CAAATCAGAA TTTATTCTTG ATCCACCAAA ACCTAAACCT
    CCTGCTTGGT TTAATGGAAT TCTAACGTGG GAAGACTTTG AATTAATAAA TCCACATAGA
    GCAAGGTTTC TAAAAGAAAT TAAGGACCTA GCTATTAAAA GGCGTCAAAT TTTAAGCAAC
    AAAAGTCTGT CAGAAGATGA AAAAAATACA AAACTACAGG AATTAATGCT AAAGAATCCA
    TCAGGTTCTG GACCACCACT TAGCATAGAG GATTTGGGTT TAAATTTTCA GTTTTGCCCT
    TCATCCAAAG TGTATGGATT TACAGCTGTA GATCTCAAAC CAGGTGGTGA AGATGAGACT
    GTAACGATGG ATAATGCAGA GGAGTATGTG GATCTCATGT TTGACTTCTG TATGCATACT
    GGTATACAGA AACAGATGGA GGCATTCAGA GATGGCTTCA ATAGAGTCTT CCCAATGGAG
    AAGCTGAGTT CTTTCAGCCA TGAAGAAGTT CAGATGATTC TTTGTGGAAA TCAGTCACCT
    TCCTGGGCAG CAGAGGATAT CATCAATTAT ACAGAACCTA AACTGGGATA TACACGGGAC
    AGCCCTGGAT TCCTTCGATT TGTGAGAGTT CTGTGTGGTA TGTCTTCAGA TGAAAGAAAG
    GCTTTCCTCC AGTTCACCAC TGGTTGTTCA ACACTCCCAC CAGGAGGACT GGCAAACCTA
    CATCCCCGGC TGACTGTTGT ACGCAAGGTT GATGCTACAG ATGCCAGTTA CCCATCTGTG
    AATACATGTG TGCATTACCT GAAGTTGCCT GAGTATTCTT CTGAGGAGAT TATGAGAGAG
    CGTCTGCTAG CTGCTACAAT GGAGAAAGGC TTCCATCTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36981
    Clone ID Related Accession (Same CDS sequence) XM_015287622.1 , XM_015287622.2
    Accession Version XM_015287622.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG TAAGTATGTA GGATCAGAAT TTGTGACTGG TTGGACTGGC
    AAAAGAGGAA GGAAGCTAAA ATCTAAACTG GAGAAGACAA AGCAGAAGGT ACGCACTATG
    GCAAGAGATT TATACGATGA TCATTTTAAA GCTGTTGAAA GCATGCCTCG AGGAGTAGTT
    GTAACACTGA GGAACATAGC AACACAGTTA GAGTCTGCGT GGGAACTTCA TACAAACAGA
    CAGTGTATTG AGGGAGAAAA CACTTGGAGA GATTTAATGA AGACTGCTTT GGAAAATTTA
    ATTGTGCTTT TGAAGGATGA GAATACCATT TCTCCATACG AAATGTGCAG TAGTGGATTA
    GTTCAATCAT TGCTTACAGT TTTAAATAAC AATGTTGATT TAGATATGAA ACAAGACTGT
    AGTCAACTAG TTGAAAGAAT AAATGTATTC AAAACAGCAT TCAGCGAAAA TGAAGATGAT
    GAAAGTCGAC CAGCAGTTGC ATTAATTCGG AAATTAATAG CAGTGTTAGA ATCTATTGAA
    CGTCTACCTC TTCACTTGTA TGATACACCA GGATCTACTT ATAATCTTCA GATATTGACA
    AGACGTTTGA GGTTTCGTTT GGAACGTGCT TCAGGGGAGA CATCCTTGAT AGATCGAACT
    GGCAGAATGC TAAAGATGGA GCCATTGGCA ACTGTTGAAT CTCTAGAACA GTATCTTCTG
    AAAATGGTTG CAAAGCAATG GTACGACTTT GATAGGGCTT CATTTGTTTT TGTACGAAAA
    CTGCGTGAAG GCCAAACATT TGTATTCAGG CATCAACATG ATTTTGATGA AAATGGAATT
    ATTTACTGGA TTGGGACAAA TGCGAAAACT GCATATGAGT GGGTAAATCC AGCAGCCTAT
    GGGTTAGTAG TGGTTACATC CTCAGAAGGA AGAAATCTGC CTTATGGCCG TTTAGAAGAC
    ATCCTAAGCC GTGACAGTTC AGCTCTCAAT TGTCATACTA ATGATGATAA GAATGCTTGG
    TTTGCCATAG ATCTTGGCCT TTGGGTTATA CCATCAGCTT ACACGTTACG CCATGCTCGA
    GGTTATGGAC GATCTGCTCT CAGAAATTGG GTTTTCCAGG TATCTAAGGA TGGACAGAAC
    TGGACTACTT TGTATACTCA TGTTGATGAT TGTAGTCTCA ACGAACCAGG GTCTACAGCA
    ACATGGCCTT TAGATCCTCC AAAGGATGAG AAACAAGGTT GGAGGCATGT TAGAATAAAA
    CAGATGGGAA AGAATGCCAG TGGGCAAACA CACTACCTCT CTCTGTCTGG ATTTGAACTT
    TATGGCACTG TGAATGGAGT ATGTGAAGAC CAGTTGGGAA AAGCTGCTAA AGAAGCTGAA
    GCAAATCTGC GAAGGCAGCG ACGTCTGGTC CGGTCTCAAG TTTTGAAATA CATGGTTCCA
    GGAGCTCGGG TAATCAGAGG AATTGATTGG AAGTGGAGAG ATCAGGATGG AAGCCCACAG
    GGTGAAGGCA CTGTTACAGG AGAATTGCAC AATGGCTGGA TTGATGTCAC CTGGGATGCT
    GGTGGCTCTA ACTCTTACCG TATGGGCGCA GAAGGAAAGT TTGACCTCAA GCTTGCACCA
    GGGTACGACC CTGATTCAGC GGCATCACCC AAACCTGTTT CATCCACTGT TTCAGGCACA
    ACGCAGTCAT GGAGCAGCTT GGTGAAAAAC AACTGTCCAG ACAAGACGAC TGCTGCTGCA
    GGCTCCTCAA GTAGAAAAGG AAGCAGCAGT TCTGTGTGTA GCGTGGCCAG TAGCAGCGAC
    ATCAGCTTGG GTTCGACCAA AATGGAGCGG AGATCAGAAA GTGTAATGGA GCAAAATATA
    GTTTCAGGCA CTGACGTCCA CGAACCAATT GTTGTTCTGT CTTCCGCTGA TAATGTACCT
    CAGGCTGAAG TAGGGTCATC TTCCAGTGCA AGCACCAGCA CCTTAACAGC GGACATGGGA
    AATGAAAATA CAGAAAGGAA GTTAGGTCCT GATAATTCGA TACGTACTCC TGGGGAGTCC
    AGTGCTATCT CCATGGGAAT TGTTAGTGTA AGTTCTCCTG ATGTTAGTTC AGTTTCCGAA
    CTGACTAATA AAGAAGCAGC ATCCCAACGA CCTCTGAGCT CTTCAGCAAG TAACAGACTT
    TCAGTTAGCT CTTTGTTGGC TGCTGGGGCG CCAATGAGCT CCAGTGCAAG CGTTCCTAAC
    TTATCATCTC GAGAAACTTC GAGCCTGGAG TCTTTTGTAC GAAGAGTGGC AAACATAGCA
    CGTACCAATG CCACAAACAA CATGAATTTA AGCCGAAGTA GCAGTGATAA CAATACTAAT
    ACTCTGGGAA GAAACGTCAT GAGTACTGCA ACGTCTCCTC TTATGGGTGC CCAAAGTTTT
    CCTAATCTGA CTACAACTGG TACCACATCA ACAGTGACCA TGTCTACATC CAGTGTTACT
    AGCAGCAGCA ATGTAGCTAC AGCAACTACA GTTTTATCTG TTGGTCAGTC GCTTAGTAAT
    ACTCTAACTA CTAGCCTAAC ATCAACATCT AGTGAGAGCG ATACAGGGCA GGAGGCAGAA
    TATTCTTTAT ATGATTTTCT GGATAGCTGT CGAGCTAGTA CTCTGTTGGC AGAGTTAGAT
    GATGATGAGG ATCTACCTGA ACCTGATGAA GAAGATGATG AAAATGAAGA TGATAACCAA
    GAAGATCAAG AATATGAGGA AGTTATGGAA GAAGAGGAGT ATGAGACCAA AGGAGGTCGT
    AGAAGAACAT GGGATGATGA TTATGTATTG AAACGACAGT TTTCTGCTCT GGTTCCTGCT
    TTTGACCCAA GACCTGGTCG AACAAATGTT CAACAAACAA CTGATTTAGA AATCCCTCCA
    CCGGGTACAC CTCATTCAGA ACTCTTGGAA GAGGTTGAGT GCATGCCTTC ACCACGCTTA
    GCACTTACGT TGAAAGTTTC AGGCCTTGGA ACAACACGAG AAGTAGAACT GCCCCTAACA
    AATTTTCGAT TCACAATATT TTACTACGTA CAGAAATTGT TACAGCTTTC CTGTAATGGC
    AGTGTGAAAT CTGACAAACT TAGACGTATT TGGGAGCCAA CATACACAAT CATGTACAGA
    GAAATGAAGG ATTCTGATAA AGAAAAAGAA AGTGGAAAAA TGGGTTGCTG GTCAGTAGAG
    CATGTGGAGC AGTACCTTGG CACTGATGAA TTACCAAAGA ATGACTTGAT AACCTACCTG
    CAGAAGAATG CAGATTCAGC TTTCCTGCGC CACTGGAAAT TAACCGGCAC TAATAAAAGT
    ATTAGGAAAA ACAGAAATTG TTCCCAGCTC ATAGCTGCAT ACAAGGATTT TTGTGAACAT
    GGATCAAAAT CTGGATTAAG CCAGGGGGCC ATTTCTACTC TTCAGAATTC TGATATTCTT
    AGTTTGGCAA AAGAGCAGCC TCAAGCCAAA GCTGGCAGTG GGCAGAACTC CTGTGGAGTA
    GAAGATGTTT TGCAGTTACT TCGCATTTTG TATATAGTTG CTAGTGACCC TTACACAGCG
    CGAACTTCTC AAGAAGAAGG AGATGAGCAT CCTCAGTTTA ACTTTCCACC AGATGAATTC
    ACAAGCAAAA AAATTACTAC AAAAATTCTG CAACAGATCG AGGAACCCTT GGCACTAGCA
    AGTGGAGCTT TGCCAGACTG GTGTGAGCAG CTAACAAGCA AGTGTCCTTT CTTAATTCCA
    TTTGAAACTA GACAGTTATA CTTCACCTGC ACAGCGTTTG GAGCATCAAG AGCAATTGTG
    TGGCTACAAA ACAGACGCGA AGCCACTGTT GAAAGAACAA GAACCACAAG CACAGTGCGA
    CGTGATGATC CGGGAGAGTT CAGAGTGGGG CGCCTGAAGC ATGAAAGGGT GAAAGTTCCG
    CGAGGTGAAT CCCTGATGGA ATGGGCAGAA AACGTCATGC AGATTCATGC AGACAGAAAA
    TCTGTTCTAG AGGTTGAGTT TTTAGGGGAG GAAGGAACGG GCCTGGGTCC TACCTTGGAA
    TTTTATGCAT TGGTAGCAGC AGAATTTCAG AGGACAGATC TGGGAGCTTG GCTTTGTGAT
    GATGATTTTC CAGATGATGA GTCTCGTCAG GTTGATATTG GTGGTGGATT AAAACCCCCT
    GGTTACTATG TACAGCGATC ATGTGGACTC TTCACAGCAC CATTCCCACA AGATAGTGAT
    GAGCTTGAAA GGATAACCAA ACTCTTTCAT TTCCTTGGAA TTTTCTTGGC TAAATGCATT
    CAAGACAATA GACTTGTGGA TTTACCCATT TCTAAGCCTT TTTTTAAACT CATGTGTATG
    GGAGACATTA AAAGCAATAT GAGTAAGTTG ATATATGAAT CACGGAGTGA CAGGGACTTG
    CACTGTACTG AAAGTCAGTC AGAGGCTTCT ACAGAAGAAG GGCACGATTC ATTGTCAGTA
    GGAAGTTTGG AAGAAGACTC CAAATCAGAA TTTATTCTTG ATCCACCAAA ACCTAAACCT
    CCTGCTTGGT TTAATGGAAT TCTAACGTGG GAAGACTTTG AATTAATAAA TCCACATAGA
    GCAAGGTTTC TAAAAGAAAT TAAGGACCTA GCTATTAAAA GGCGTCAAAT TTTAAGCAAC
    AAAAGTCTGT CAGAAGATGA AAAAAATACA AAACTACAGG AATTAATGCT AAAGAATCCA
    TCAGGTTCTG GACCACCACT TAGCATAGAG GATTTGGGTT TAAATTTTCA GTTTTGCCCT
    TCATCCAAAG TGTATGGATT TACAGCTGTA GATCTCAAAC CAGGTGGTGA AGATGAGACT
    GTAACGATGG ATAATGCAGA GGAGTATGTG GATCTCATGT TTGACTTCTG TATGCATACT
    GGTATACAGA AACAGATGGA GGCATTCAGA GATGGCTTCA ATAGAGTCTT CCCAATGGAG
    AAGCTGAGTT CTTTCAGCCA TGAAGAAGTT CAGATGATTC TTTGTGGAAA TCAGTCACCT
    TCCTGGGCAG CAGAGGATAT CATCAATTAT ACAGAACCTA AACTGGGATA TACACGGGAC
    AGCCCTGGAT TCCTTCGATT TGTGAGAGTT CTGTGTGGTA TGTCTTCAGA TGAAAGAAAG
    GCTTTCCTCC AGTTCACCAC TGGTTGTTCA ACACTCCCAC CAGGAGGACT GGCAAACCTA
    CATCCCCGGC TGACTGTTGT ACGCAAGGTT GATGCTACAG ATGCCAGTTA CCCATCTGTG
    AATACATGTG TGCATTACCT GAAGTTGCCT GAGTATTCTT CTGAGGAGAT TATGAGAGAG
    CGTCTGCTAG CTGCTACAAT GGAGAAAGGC TTCCATCTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143108.1
    CDS309..8033
    Translation

    Target ORF information:

    RefSeq Version XM_015287622.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287622.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG TAAGTATGTA GGATCAGAAT TTGTGACTGG TTGGACTGGC
    AAAAGAGGAA GGAAGCTAAA ATCTAAACTG GAGAAGACAA AGCAGAAGGT ACGCACTATG
    GCAAGAGATT TATACGATGA TCATTTTAAA GCTGTTGAAA GCATGCCTCG AGGAGTAGTT
    GTAACACTGA GGAACATAGC AACACAGTTA GAGTCTGCGT GGGAACTTCA TACAAACAGA
    CAGTGTATTG AGGGAGAAAA CACTTGGAGA GATTTAATGA AGACTGCTTT GGAAAATTTA
    ATTGTGCTTT TGAAGGATGA GAATACCATT TCTCCATACG AAATGTGCAG TAGTGGATTA
    GTTCAATCAT TGCTTACAGT TTTAAATAAC AATGTTGATT TAGATATGAA ACAAGACTGT
    AGTCAACTAG TTGAAAGAAT AAATGTATTC AAAACAGCAT TCAGCGAAAA TGAAGATGAT
    GAAAGTCGAC CAGCAGTTGC ATTAATTCGG AAATTAATAG CAGTGTTAGA ATCTATTGAA
    CGTCTACCTC TTCACTTGTA TGATACACCA GGATCTACTT ATAATCTTCA GATATTGACA
    AGACGTTTGA GGTTTCGTTT GGAACGTGCT TCAGGGGAGA CATCCTTGAT AGATCGAACT
    GGCAGAATGC TAAAGATGGA GCCATTGGCA ACTGTTGAAT CTCTAGAACA GTATCTTCTG
    AAAATGGTTG CAAAGCAATG GTACGACTTT GATAGGGCTT CATTTGTTTT TGTACGAAAA
    CTGCGTGAAG GCCAAACATT TGTATTCAGG CATCAACATG ATTTTGATGA AAATGGAATT
    ATTTACTGGA TTGGGACAAA TGCGAAAACT GCATATGAGT GGGTAAATCC AGCAGCCTAT
    GGGTTAGTAG TGGTTACATC CTCAGAAGGA AGAAATCTGC CTTATGGCCG TTTAGAAGAC
    ATCCTAAGCC GTGACAGTTC AGCTCTCAAT TGTCATACTA ATGATGATAA GAATGCTTGG
    TTTGCCATAG ATCTTGGCCT TTGGGTTATA CCATCAGCTT ACACGTTACG CCATGCTCGA
    GGTTATGGAC GATCTGCTCT CAGAAATTGG GTTTTCCAGG TATCTAAGGA TGGACAGAAC
    TGGACTACTT TGTATACTCA TGTTGATGAT TGTAGTCTCA ACGAACCAGG GTCTACAGCA
    ACATGGCCTT TAGATCCTCC AAAGGATGAG AAACAAGGTT GGAGGCATGT TAGAATAAAA
    CAGATGGGAA AGAATGCCAG TGGGCAAACA CACTACCTCT CTCTGTCTGG ATTTGAACTT
    TATGGCACTG TGAATGGAGT ATGTGAAGAC CAGTTGGGAA AAGCTGCTAA AGAAGCTGAA
    GCAAATCTGC GAAGGCAGCG ACGTCTGGTC CGGTCTCAAG TTTTGAAATA CATGGTTCCA
    GGAGCTCGGG TAATCAGAGG AATTGATTGG AAGTGGAGAG ATCAGGATGG AAGCCCACAG
    GGTGAAGGCA CTGTTACAGG AGAATTGCAC AATGGCTGGA TTGATGTCAC CTGGGATGCT
    GGTGGCTCTA ACTCTTACCG TATGGGCGCA GAAGGAAAGT TTGACCTCAA GCTTGCACCA
    GGGTACGACC CTGATTCAGC GGCATCACCC AAACCTGTTT CATCCACTGT TTCAGGCACA
    ACGCAGTCAT GGAGCAGCTT GGTGAAAAAC AACTGTCCAG ACAAGACGAC TGCTGCTGCA
    GGCTCCTCAA GTAGAAAAGG AAGCAGCAGT TCTGTGTGTA GCGTGGCCAG TAGCAGCGAC
    ATCAGCTTGG GTTCGACCAA AATGGAGCGG AGATCAGAAA GTGTAATGGA GCAAAATATA
    GTTTCAGGCA CTGACGTCCA CGAACCAATT GTTGTTCTGT CTTCCGCTGA TAATGTACCT
    CAGGCTGAAG TAGGGTCATC TTCCAGTGCA AGCACCAGCA CCTTAACAGC GGACATGGGA
    AATGAAAATA CAGAAAGGAA GTTAGGTCCT GATAATTCGA TACGTACTCC TGGGGAGTCC
    AGTGCTATCT CCATGGGAAT TGTTAGTGTA AGTTCTCCTG ATGTTAGTTC AGTTTCCGAA
    CTGACTAATA AAGAAGCAGC ATCCCAACGA CCTCTGAGCT CTTCAGCAAG TAACAGACTT
    TCAGTTAGCT CTTTGTTGGC TGCTGGGGCG CCAATGAGCT CCAGTGCAAG CGTTCCTAAC
    TTATCATCTC GAGAAACTTC GAGCCTGGAG TCTTTTGTAC GAAGAGTGGC AAACATAGCA
    CGTACCAATG CCACAAACAA CATGAATTTA AGCCGAAGTA GCAGTGATAA CAATACTAAT
    ACTCTGGGAA GAAACGTCAT GAGTACTGCA ACGTCTCCTC TTATGGGTGC CCAAAGTTTT
    CCTAATCTGA CTACAACTGG TACCACATCA ACAGTGACCA TGTCTACATC CAGTGTTACT
    AGCAGCAGCA ATGTAGCTAC AGCAACTACA GTTTTATCTG TTGGTCAGTC GCTTAGTAAT
    ACTCTAACTA CTAGCCTAAC ATCAACATCT AGTGAGAGCG ATACAGGGCA GGAGGCAGAA
    TATTCTTTAT ATGATTTTCT GGATAGCTGT CGAGCTAGTA CTCTGTTGGC AGAGTTAGAT
    GATGATGAGG ATCTACCTGA ACCTGATGAA GAAGATGATG AAAATGAAGA TGATAACCAA
    GAAGATCAAG AATATGAGGA AGTTATGGAA GAAGAGGAGT ATGAGACCAA AGGAGGTCGT
    AGAAGAACAT GGGATGATGA TTATGTATTG AAACGACAGT TTTCTGCTCT GGTTCCTGCT
    TTTGACCCAA GACCTGGTCG AACAAATGTT CAACAAACAA CTGATTTAGA AATCCCTCCA
    CCGGGTACAC CTCATTCAGA ACTCTTGGAA GAGGTTGAGT GCATGCCTTC ACCACGCTTA
    GCACTTACGT TGAAAGTTTC AGGCCTTGGA ACAACACGAG AAGTAGAACT GCCCCTAACA
    AATTTTCGAT TCACAATATT TTACTACGTA CAGAAATTGT TACAGCTTTC CTGTAATGGC
    AGTGTGAAAT CTGACAAACT TAGACGTATT TGGGAGCCAA CATACACAAT CATGTACAGA
    GAAATGAAGG ATTCTGATAA AGAAAAAGAA AGTGGAAAAA TGGGTTGCTG GTCAGTAGAG
    CATGTGGAGC AGTACCTTGG CACTGATGAA TTACCAAAGA ATGACTTGAT AACCTACCTG
    CAGAAGAATG CAGATTCAGC TTTCCTGCGC CACTGGAAAT TAACCGGCAC TAATAAAAGT
    ATTAGGAAAA ACAGAAATTG TTCCCAGCTC ATAGCTGCAT ACAAGGATTT TTGTGAACAT
    GGATCAAAAT CTGGATTAAG CCAGGGGGCC ATTTCTACTC TTCAGAATTC TGATATTCTT
    AGTTTGGCAA AAGAGCAGCC TCAAGCCAAA GCTGGCAGTG GGCAGAACTC CTGTGGAGTA
    GAAGATGTTT TGCAGTTACT TCGCATTTTG TATATAGTTG CTAGTGACCC TTACACAGCG
    CGAACTTCTC AAGAAGAAGG AGATGAGCAT CCTCAGTTTA ACTTTCCACC AGATGAATTC
    ACAAGCAAAA AAATTACTAC AAAAATTCTG CAACAGATCG AGGAACCCTT GGCACTAGCA
    AGTGGAGCTT TGCCAGACTG GTGTGAGCAG CTAACAAGCA AGTGTCCTTT CTTAATTCCA
    TTTGAAACTA GACAGTTATA CTTCACCTGC ACAGCGTTTG GAGCATCAAG AGCAATTGTG
    TGGCTACAAA ACAGACGCGA AGCCACTGTT GAAAGAACAA GAACCACAAG CACAGTGCGA
    CGTGATGATC CGGGAGAGTT CAGAGTGGGG CGCCTGAAGC ATGAAAGGGT GAAAGTTCCG
    CGAGGTGAAT CCCTGATGGA ATGGGCAGAA AACGTCATGC AGATTCATGC AGACAGAAAA
    TCTGTTCTAG AGGTTGAGTT TTTAGGGGAG GAAGGAACGG GCCTGGGTCC TACCTTGGAA
    TTTTATGCAT TGGTAGCAGC AGAATTTCAG AGGACAGATC TGGGAGCTTG GCTTTGTGAT
    GATGATTTTC CAGATGATGA GTCTCGTCAG GTTGATATTG GTGGTGGATT AAAACCCCCT
    GGTTACTATG TACAGCGATC ATGTGGACTC TTCACAGCAC CATTCCCACA AGATAGTGAT
    GAGCTTGAAA GGATAACCAA ACTCTTTCAT TTCCTTGGAA TTTTCTTGGC TAAATGCATT
    CAAGACAATA GACTTGTGGA TTTACCCATT TCTAAGCCTT TTTTTAAACT CATGTGTATG
    GGAGACATTA AAAGCAATAT GAGTAAGTTG ATATATGAAT CACGGAGTGA CAGGGACTTG
    CACTGTACTG AAAGTCAGTC AGAGGCTTCT ACAGAAGAAG GGCACGATTC ATTGTCAGTA
    GGAAGTTTGG AAGAAGACTC CAAATCAGAA TTTATTCTTG ATCCACCAAA ACCTAAACCT
    CCTGCTTGGT TTAATGGAAT TCTAACGTGG GAAGACTTTG AATTAATAAA TCCACATAGA
    GCAAGGTTTC TAAAAGAAAT TAAGGACCTA GCTATTAAAA GGCGTCAAAT TTTAAGCAAC
    AAAAGTCTGT CAGAAGATGA AAAAAATACA AAACTACAGG AATTAATGCT AAAGAATCCA
    TCAGGTTCTG GACCACCACT TAGCATAGAG GATTTGGGTT TAAATTTTCA GTTTTGCCCT
    TCATCCAAAG TGTATGGATT TACAGCTGTA GATCTCAAAC CAGGTGGTGA AGATGAGACT
    GTAACGATGG ATAATGCAGA GGAGTATGTG GATCTCATGT TTGACTTCTG TATGCATACT
    GGTATACAGA AACAGATGGA GGCATTCAGA GATGGCTTCA ATAGAGTCTT CCCAATGGAG
    AAGCTGAGTT CTTTCAGCCA TGAAGAAGTT CAGATGATTC TTTGTGGAAA TCAGTCACCT
    TCCTGGGCAG CAGAGGATAT CATCAATTAT ACAGAACCTA AACTGGGATA TACACGGGAC
    AGCCCTGGAT TCCTTCGATT TGTGAGAGTT CTGTGTGGTA TGTCTTCAGA TGAAAGAAAG
    GCTTTCCTCC AGTTCACCAC TGGTTGTTCA ACACTCCCAC CAGGAGGACT GGCAAACCTA
    CATCCCCGGC TGACTGTTGT ACGCAAGGTT GATGCTACAG ATGCCAGTTA CCCATCTGTG
    AATACATGTG TGCATTACCT GAAGTTGCCT GAGTATTCTT CTGAGGAGAT TATGAGAGAG
    CGTCTGCTAG CTGCTACAAT GGAGAAAGGC TTCCATCTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36982
    Clone ID Related Accession (Same CDS sequence) XM_015287623.2 , XM_015287623.1
    Accession Version XM_015287623.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCACAACG
    CAGTCATGGA GCAGCTTGGT GAAAAACAAC TGTCCAGACA AGACGACTGC TGCTGCAGGC
    TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT GTGTGTAGCG TGGCCAGTAG CAGCGACATC
    AGCTTGGGTT CGACCAAAAT GGAGCGGAGA TCAGAAAGTG TAATGGAGCA AAATATAGTT
    TCAGGCACTG ACGTCCACGA ACCAATTGTT GTTCTGTCTT CCGCTGATAA TGTACCTCAG
    GCTGAAGTAG GGTCATCTTC CAGTGCAAGC ACCAGCACCT TAACAGCGGA CATGGGAAAT
    GAAAATACAG AAAGGAAGTT AGGTCCTGAT AATTCGATAC GTACTCCTGG GGAGTCCAGT
    GCTATCTCCA TGGGAATTGT TAGTGTAAGT TCTCCTGATG TTAGTTCAGT TTCCGAACTG
    ACTAATAAAG AAGCAGCATC CCAACGACCT CTGAGCTCTT CAGCAAGTAA CAGACTTTCA
    GTTAGCTCTT TGTTGGCTGC TGGGGCGCCA ATGAGCTCCA GTGCAAGCGT TCCTAACTTA
    TCATCTCGAG AAACTTCGAG CCTGGAGTCT TTTGTACGAA GAGTGGCAAA CATAGCACGT
    ACCAATGCCA CAAACAACAT GAATTTAAGC CGAAGTAGCA GTGATAACAA TACTAATACT
    CTGGGAAGAA ACGTCATGAG TACTGCAACG TCTCCTCTTA TGGGTGCCCA AAGTTTTCCT
    AATCTGACTA CAACTGGTAC CACATCAACA GTGACCATGT CTACATCCAG TGTTACTAGC
    AGCAGCAATG TAGCTACAGC AACTACAGTT TTATCTGTTG GTCAGTCGCT TAGTAATACT
    CTAACTACTA GCCTAACATC AACATCTAGT GAGAGCGATA CAGGGCAGGA GGCAGAATAT
    TCTTTATATG ATTTTCTGGA TAGCTGTCGA GCTAGTACTC TGTTGGCAGA GTTAGATGAT
    GATGAGGATC TACCTGAACC TGATGAAGAA GATGATGAAA ATGAAGATGA TAACCAAGAA
    GATCAAGAAT ATGAGGAAGT TATGGAAGAA GAGGAGTATG AGACCAAAGG AGGTCGTAGA
    AGAACATGGG ATGATGATTA TGTATTGAAA CGACAGTTTT CTGCTCTGGT TCCTGCTTTT
    GACCCAAGAC CTGGTCGAAC AAATGTTCAA CAAACAACTG ATTTAGAAAT CCCTCCACCG
    GGTACACCTC ATTCAGAACT CTTGGAAGAG GTTGAGTGCA TGCCTTCACC ACGCTTAGCA
    CTTACGTTGA AAGTTTCAGG CCTTGGAACA ACACGAGAAG TAGAACTGCC CCTAACAAAT
    TTTCGATTCA CAATATTTTA CTACGTACAG AAATTGTTAC AGCTTTCCTG TAATGGCAGT
    GTGAAATCTG ACAAACTTAG ACGTATTTGG GAGCCAACAT ACACAATCAT GTACAGAGAA
    ATGAAGGATT CTGATAAAGA AAAAGAAAGT GGAAAAATGG GTTGCTGGTC AGTAGAGCAT
    GTGGAGCAGT ACCTTGGCAC TGATGAATTA CCAAAGAATG ACTTGATAAC CTACCTGCAG
    AAGAATGCAG ATTCAGCTTT CCTGCGCCAC TGGAAATTAA CCGGCACTAA TAAAAGTATT
    AGGAAAAACA GAAATTGTTC CCAGCTCATA GCTGCATACA AGGATTTTTG TGAACATGGA
    TCAAAATCTG GATTAAGCCA GGGGGCCATT TCTACTCTTC AGAATTCTGA TATTCTTAGT
    TTGGCAAAAG AGCAGCCTCA AGCCAAAGCT GGCAGTGGGC AGAACTCCTG TGGAGTAGAA
    GATGTTTTGC AGTTACTTCG CATTTTGTAT ATAGTTGCTA GTGACCCTTA CACAGCGCGA
    ACTTCTCAAG AAGAAGGAGA TGAGCATCCT CAGTTTAACT TTCCACCAGA TGAATTCACA
    AGCAAAAAAA TTACTACAAA AATTCTGCAA CAGATCGAGG AACCCTTGGC ACTAGCAAGT
    GGAGCTTTGC CAGACTGGTG TGAGCAGCTA ACAAGCAAGT GTCCTTTCTT AATTCCATTT
    GAAACTAGAC AGTTATACTT CACCTGCACA GCGTTTGGAG CATCAAGAGC AATTGTGTGG
    CTACAAAACA GACGCGAAGC CACTGTTGAA AGAACAAGAA CCACAAGCAC AGTGCGACGT
    GATGATCCGG GAGAGTTCAG AGTGGGGCGC CTGAAGCATG AAAGGGTGAA AGTTCCGCGA
    GGTGAATCCC TGATGGAATG GGCAGAAAAC GTCATGCAGA TTCATGCAGA CAGAAAATCT
    GTTCTAGAGG TTGAGTTTTT AGGGGAGGAA GGAACGGGCC TGGGTCCTAC CTTGGAATTT
    TATGCATTGG TAGCAGCAGA ATTTCAGAGG ACAGATCTGG GAGCTTGGCT TTGTGATGAT
    GATTTTCCAG ATGATGAGTC TCGTCAGGTT GATATTGGTG GTGGATTAAA ACCCCCTGGT
    TACTATGTAC AGCGATCATG TGGACTCTTC ACAGCACCAT TCCCACAAGA TAGTGATGAG
    CTTGAAAGGA TAACCAAACT CTTTCATTTC CTTGGAATTT TCTTGGCTAA ATGCATTCAA
    GACAATAGAC TTGTGGATTT ACCCATTTCT AAGCCTTTTT TTAAACTCAT GTGTATGGGA
    GACATTAAAA GCAATATGAG TAAGTTGATA TATGAATCAC GGAGTGACAG GGACTTGCAC
    TGTACTGAAA GTCAGTCAGA GGCTTCTACA GAAGAAGGGC ACGATTCATT GTCAGTAGGA
    AGTTTGGAAG AAGACTCCAA ATCAGAATTT ATTCTTGATC CACCAAAACC TAAACCTCCT
    GCTTGGTTTA ATGGAATTCT AACGTGGGAA GACTTTGAAT TAATAAATCC ACATAGAGCA
    AGGTTTCTAA AAGAAATTAA GGACCTAGCT ATTAAAAGGC GTCAAATTTT AAGCAACAAA
    AGTCTGTCAG AAGATGAAAA AAATACAAAA CTACAGGAAT TAATGCTAAA GAATCCATCA
    GGTTCTGGAC CACCACTTAG CATAGAGGAT TTGGGTTTAA ATTTTCAGTT TTGCCCTTCA
    TCCAAAGTGT ATGGATTTAC AGCTGTAGAT CTCAAACCAG GTGGTGAAGA TGAGACTGTA
    ACGATGGATA ATGCAGAGGA GTATGTGGAT CTCATGTTTG ACTTCTGTAT GCATACTGGT
    ATACAGAAAC AGATGGAGGC ATTCAGAGAT GGCTTCAATA GAGTCTTCCC AATGGAGAAG
    CTGAGTTCTT TCAGCCATGA AGAAGTTCAG ATGATTCTTT GTGGAAATCA GTCACCTTCC
    TGGGCAGCAG AGGATATCAT CAATTATACA GAACCTAAAC TGGGATATAC ACGGGACAGC
    CCTGGATTCC TTCGATTTGT GAGAGTTCTG TGTGGTATGT CTTCAGATGA AAGAAAGGCT
    TTCCTCCAGT TCACCACTGG TTGTTCAACA CTCCCACCAG GAGGACTGGC AAACCTACAT
    CCCCGGCTGA CTGTTGTACG CAAGGTTGAT GCTACAGATG CCAGTTACCC ATCTGTGAAT
    ACATGTGTGC ATTACCTGAA GTTGCCTGAG TATTCTTCTG AGGAGATTAT GAGAGAGCGT
    CTGCTAGCTG CTACAATGGA GAAAGGCTTC CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143109.1
    CDS309..7910
    Translation

    Target ORF information:

    RefSeq Version XM_015287623.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287623.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCACAACG
    CAGTCATGGA GCAGCTTGGT GAAAAACAAC TGTCCAGACA AGACGACTGC TGCTGCAGGC
    TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT GTGTGTAGCG TGGCCAGTAG CAGCGACATC
    AGCTTGGGTT CGACCAAAAT GGAGCGGAGA TCAGAAAGTG TAATGGAGCA AAATATAGTT
    TCAGGCACTG ACGTCCACGA ACCAATTGTT GTTCTGTCTT CCGCTGATAA TGTACCTCAG
    GCTGAAGTAG GGTCATCTTC CAGTGCAAGC ACCAGCACCT TAACAGCGGA CATGGGAAAT
    GAAAATACAG AAAGGAAGTT AGGTCCTGAT AATTCGATAC GTACTCCTGG GGAGTCCAGT
    GCTATCTCCA TGGGAATTGT TAGTGTAAGT TCTCCTGATG TTAGTTCAGT TTCCGAACTG
    ACTAATAAAG AAGCAGCATC CCAACGACCT CTGAGCTCTT CAGCAAGTAA CAGACTTTCA
    GTTAGCTCTT TGTTGGCTGC TGGGGCGCCA ATGAGCTCCA GTGCAAGCGT TCCTAACTTA
    TCATCTCGAG AAACTTCGAG CCTGGAGTCT TTTGTACGAA GAGTGGCAAA CATAGCACGT
    ACCAATGCCA CAAACAACAT GAATTTAAGC CGAAGTAGCA GTGATAACAA TACTAATACT
    CTGGGAAGAA ACGTCATGAG TACTGCAACG TCTCCTCTTA TGGGTGCCCA AAGTTTTCCT
    AATCTGACTA CAACTGGTAC CACATCAACA GTGACCATGT CTACATCCAG TGTTACTAGC
    AGCAGCAATG TAGCTACAGC AACTACAGTT TTATCTGTTG GTCAGTCGCT TAGTAATACT
    CTAACTACTA GCCTAACATC AACATCTAGT GAGAGCGATA CAGGGCAGGA GGCAGAATAT
    TCTTTATATG ATTTTCTGGA TAGCTGTCGA GCTAGTACTC TGTTGGCAGA GTTAGATGAT
    GATGAGGATC TACCTGAACC TGATGAAGAA GATGATGAAA ATGAAGATGA TAACCAAGAA
    GATCAAGAAT ATGAGGAAGT TATGGAAGAA GAGGAGTATG AGACCAAAGG AGGTCGTAGA
    AGAACATGGG ATGATGATTA TGTATTGAAA CGACAGTTTT CTGCTCTGGT TCCTGCTTTT
    GACCCAAGAC CTGGTCGAAC AAATGTTCAA CAAACAACTG ATTTAGAAAT CCCTCCACCG
    GGTACACCTC ATTCAGAACT CTTGGAAGAG GTTGAGTGCA TGCCTTCACC ACGCTTAGCA
    CTTACGTTGA AAGTTTCAGG CCTTGGAACA ACACGAGAAG TAGAACTGCC CCTAACAAAT
    TTTCGATTCA CAATATTTTA CTACGTACAG AAATTGTTAC AGCTTTCCTG TAATGGCAGT
    GTGAAATCTG ACAAACTTAG ACGTATTTGG GAGCCAACAT ACACAATCAT GTACAGAGAA
    ATGAAGGATT CTGATAAAGA AAAAGAAAGT GGAAAAATGG GTTGCTGGTC AGTAGAGCAT
    GTGGAGCAGT ACCTTGGCAC TGATGAATTA CCAAAGAATG ACTTGATAAC CTACCTGCAG
    AAGAATGCAG ATTCAGCTTT CCTGCGCCAC TGGAAATTAA CCGGCACTAA TAAAAGTATT
    AGGAAAAACA GAAATTGTTC CCAGCTCATA GCTGCATACA AGGATTTTTG TGAACATGGA
    TCAAAATCTG GATTAAGCCA GGGGGCCATT TCTACTCTTC AGAATTCTGA TATTCTTAGT
    TTGGCAAAAG AGCAGCCTCA AGCCAAAGCT GGCAGTGGGC AGAACTCCTG TGGAGTAGAA
    GATGTTTTGC AGTTACTTCG CATTTTGTAT ATAGTTGCTA GTGACCCTTA CACAGCGCGA
    ACTTCTCAAG AAGAAGGAGA TGAGCATCCT CAGTTTAACT TTCCACCAGA TGAATTCACA
    AGCAAAAAAA TTACTACAAA AATTCTGCAA CAGATCGAGG AACCCTTGGC ACTAGCAAGT
    GGAGCTTTGC CAGACTGGTG TGAGCAGCTA ACAAGCAAGT GTCCTTTCTT AATTCCATTT
    GAAACTAGAC AGTTATACTT CACCTGCACA GCGTTTGGAG CATCAAGAGC AATTGTGTGG
    CTACAAAACA GACGCGAAGC CACTGTTGAA AGAACAAGAA CCACAAGCAC AGTGCGACGT
    GATGATCCGG GAGAGTTCAG AGTGGGGCGC CTGAAGCATG AAAGGGTGAA AGTTCCGCGA
    GGTGAATCCC TGATGGAATG GGCAGAAAAC GTCATGCAGA TTCATGCAGA CAGAAAATCT
    GTTCTAGAGG TTGAGTTTTT AGGGGAGGAA GGAACGGGCC TGGGTCCTAC CTTGGAATTT
    TATGCATTGG TAGCAGCAGA ATTTCAGAGG ACAGATCTGG GAGCTTGGCT TTGTGATGAT
    GATTTTCCAG ATGATGAGTC TCGTCAGGTT GATATTGGTG GTGGATTAAA ACCCCCTGGT
    TACTATGTAC AGCGATCATG TGGACTCTTC ACAGCACCAT TCCCACAAGA TAGTGATGAG
    CTTGAAAGGA TAACCAAACT CTTTCATTTC CTTGGAATTT TCTTGGCTAA ATGCATTCAA
    GACAATAGAC TTGTGGATTT ACCCATTTCT AAGCCTTTTT TTAAACTCAT GTGTATGGGA
    GACATTAAAA GCAATATGAG TAAGTTGATA TATGAATCAC GGAGTGACAG GGACTTGCAC
    TGTACTGAAA GTCAGTCAGA GGCTTCTACA GAAGAAGGGC ACGATTCATT GTCAGTAGGA
    AGTTTGGAAG AAGACTCCAA ATCAGAATTT ATTCTTGATC CACCAAAACC TAAACCTCCT
    GCTTGGTTTA ATGGAATTCT AACGTGGGAA GACTTTGAAT TAATAAATCC ACATAGAGCA
    AGGTTTCTAA AAGAAATTAA GGACCTAGCT ATTAAAAGGC GTCAAATTTT AAGCAACAAA
    AGTCTGTCAG AAGATGAAAA AAATACAAAA CTACAGGAAT TAATGCTAAA GAATCCATCA
    GGTTCTGGAC CACCACTTAG CATAGAGGAT TTGGGTTTAA ATTTTCAGTT TTGCCCTTCA
    TCCAAAGTGT ATGGATTTAC AGCTGTAGAT CTCAAACCAG GTGGTGAAGA TGAGACTGTA
    ACGATGGATA ATGCAGAGGA GTATGTGGAT CTCATGTTTG ACTTCTGTAT GCATACTGGT
    ATACAGAAAC AGATGGAGGC ATTCAGAGAT GGCTTCAATA GAGTCTTCCC AATGGAGAAG
    CTGAGTTCTT TCAGCCATGA AGAAGTTCAG ATGATTCTTT GTGGAAATCA GTCACCTTCC
    TGGGCAGCAG AGGATATCAT CAATTATACA GAACCTAAAC TGGGATATAC ACGGGACAGC
    CCTGGATTCC TTCGATTTGT GAGAGTTCTG TGTGGTATGT CTTCAGATGA AAGAAAGGCT
    TTCCTCCAGT TCACCACTGG TTGTTCAACA CTCCCACCAG GAGGACTGGC AAACCTACAT
    CCCCGGCTGA CTGTTGTACG CAAGGTTGAT GCTACAGATG CCAGTTACCC ATCTGTGAAT
    ACATGTGTGC ATTACCTGAA GTTGCCTGAG TATTCTTCTG AGGAGATTAT GAGAGAGCGT
    CTGCTAGCTG CTACAATGGA GAAAGGCTTC CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36982
    Clone ID Related Accession (Same CDS sequence) XM_015287623.2 , XM_015287623.1
    Accession Version XM_015287623.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
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    2941
    3001
    3061
    3121
    3181
    3241
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    3421
    3481
    3541
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCACAACG
    CAGTCATGGA GCAGCTTGGT GAAAAACAAC TGTCCAGACA AGACGACTGC TGCTGCAGGC
    TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT GTGTGTAGCG TGGCCAGTAG CAGCGACATC
    AGCTTGGGTT CGACCAAAAT GGAGCGGAGA TCAGAAAGTG TAATGGAGCA AAATATAGTT
    TCAGGCACTG ACGTCCACGA ACCAATTGTT GTTCTGTCTT CCGCTGATAA TGTACCTCAG
    GCTGAAGTAG GGTCATCTTC CAGTGCAAGC ACCAGCACCT TAACAGCGGA CATGGGAAAT
    GAAAATACAG AAAGGAAGTT AGGTCCTGAT AATTCGATAC GTACTCCTGG GGAGTCCAGT
    GCTATCTCCA TGGGAATTGT TAGTGTAAGT TCTCCTGATG TTAGTTCAGT TTCCGAACTG
    ACTAATAAAG AAGCAGCATC CCAACGACCT CTGAGCTCTT CAGCAAGTAA CAGACTTTCA
    GTTAGCTCTT TGTTGGCTGC TGGGGCGCCA ATGAGCTCCA GTGCAAGCGT TCCTAACTTA
    TCATCTCGAG AAACTTCGAG CCTGGAGTCT TTTGTACGAA GAGTGGCAAA CATAGCACGT
    ACCAATGCCA CAAACAACAT GAATTTAAGC CGAAGTAGCA GTGATAACAA TACTAATACT
    CTGGGAAGAA ACGTCATGAG TACTGCAACG TCTCCTCTTA TGGGTGCCCA AAGTTTTCCT
    AATCTGACTA CAACTGGTAC CACATCAACA GTGACCATGT CTACATCCAG TGTTACTAGC
    AGCAGCAATG TAGCTACAGC AACTACAGTT TTATCTGTTG GTCAGTCGCT TAGTAATACT
    CTAACTACTA GCCTAACATC AACATCTAGT GAGAGCGATA CAGGGCAGGA GGCAGAATAT
    TCTTTATATG ATTTTCTGGA TAGCTGTCGA GCTAGTACTC TGTTGGCAGA GTTAGATGAT
    GATGAGGATC TACCTGAACC TGATGAAGAA GATGATGAAA ATGAAGATGA TAACCAAGAA
    GATCAAGAAT ATGAGGAAGT TATGGAAGAA GAGGAGTATG AGACCAAAGG AGGTCGTAGA
    AGAACATGGG ATGATGATTA TGTATTGAAA CGACAGTTTT CTGCTCTGGT TCCTGCTTTT
    GACCCAAGAC CTGGTCGAAC AAATGTTCAA CAAACAACTG ATTTAGAAAT CCCTCCACCG
    GGTACACCTC ATTCAGAACT CTTGGAAGAG GTTGAGTGCA TGCCTTCACC ACGCTTAGCA
    CTTACGTTGA AAGTTTCAGG CCTTGGAACA ACACGAGAAG TAGAACTGCC CCTAACAAAT
    TTTCGATTCA CAATATTTTA CTACGTACAG AAATTGTTAC AGCTTTCCTG TAATGGCAGT
    GTGAAATCTG ACAAACTTAG ACGTATTTGG GAGCCAACAT ACACAATCAT GTACAGAGAA
    ATGAAGGATT CTGATAAAGA AAAAGAAAGT GGAAAAATGG GTTGCTGGTC AGTAGAGCAT
    GTGGAGCAGT ACCTTGGCAC TGATGAATTA CCAAAGAATG ACTTGATAAC CTACCTGCAG
    AAGAATGCAG ATTCAGCTTT CCTGCGCCAC TGGAAATTAA CCGGCACTAA TAAAAGTATT
    AGGAAAAACA GAAATTGTTC CCAGCTCATA GCTGCATACA AGGATTTTTG TGAACATGGA
    TCAAAATCTG GATTAAGCCA GGGGGCCATT TCTACTCTTC AGAATTCTGA TATTCTTAGT
    TTGGCAAAAG AGCAGCCTCA AGCCAAAGCT GGCAGTGGGC AGAACTCCTG TGGAGTAGAA
    GATGTTTTGC AGTTACTTCG CATTTTGTAT ATAGTTGCTA GTGACCCTTA CACAGCGCGA
    ACTTCTCAAG AAGAAGGAGA TGAGCATCCT CAGTTTAACT TTCCACCAGA TGAATTCACA
    AGCAAAAAAA TTACTACAAA AATTCTGCAA CAGATCGAGG AACCCTTGGC ACTAGCAAGT
    GGAGCTTTGC CAGACTGGTG TGAGCAGCTA ACAAGCAAGT GTCCTTTCTT AATTCCATTT
    GAAACTAGAC AGTTATACTT CACCTGCACA GCGTTTGGAG CATCAAGAGC AATTGTGTGG
    CTACAAAACA GACGCGAAGC CACTGTTGAA AGAACAAGAA CCACAAGCAC AGTGCGACGT
    GATGATCCGG GAGAGTTCAG AGTGGGGCGC CTGAAGCATG AAAGGGTGAA AGTTCCGCGA
    GGTGAATCCC TGATGGAATG GGCAGAAAAC GTCATGCAGA TTCATGCAGA CAGAAAATCT
    GTTCTAGAGG TTGAGTTTTT AGGGGAGGAA GGAACGGGCC TGGGTCCTAC CTTGGAATTT
    TATGCATTGG TAGCAGCAGA ATTTCAGAGG ACAGATCTGG GAGCTTGGCT TTGTGATGAT
    GATTTTCCAG ATGATGAGTC TCGTCAGGTT GATATTGGTG GTGGATTAAA ACCCCCTGGT
    TACTATGTAC AGCGATCATG TGGACTCTTC ACAGCACCAT TCCCACAAGA TAGTGATGAG
    CTTGAAAGGA TAACCAAACT CTTTCATTTC CTTGGAATTT TCTTGGCTAA ATGCATTCAA
    GACAATAGAC TTGTGGATTT ACCCATTTCT AAGCCTTTTT TTAAACTCAT GTGTATGGGA
    GACATTAAAA GCAATATGAG TAAGTTGATA TATGAATCAC GGAGTGACAG GGACTTGCAC
    TGTACTGAAA GTCAGTCAGA GGCTTCTACA GAAGAAGGGC ACGATTCATT GTCAGTAGGA
    AGTTTGGAAG AAGACTCCAA ATCAGAATTT ATTCTTGATC CACCAAAACC TAAACCTCCT
    GCTTGGTTTA ATGGAATTCT AACGTGGGAA GACTTTGAAT TAATAAATCC ACATAGAGCA
    AGGTTTCTAA AAGAAATTAA GGACCTAGCT ATTAAAAGGC GTCAAATTTT AAGCAACAAA
    AGTCTGTCAG AAGATGAAAA AAATACAAAA CTACAGGAAT TAATGCTAAA GAATCCATCA
    GGTTCTGGAC CACCACTTAG CATAGAGGAT TTGGGTTTAA ATTTTCAGTT TTGCCCTTCA
    TCCAAAGTGT ATGGATTTAC AGCTGTAGAT CTCAAACCAG GTGGTGAAGA TGAGACTGTA
    ACGATGGATA ATGCAGAGGA GTATGTGGAT CTCATGTTTG ACTTCTGTAT GCATACTGGT
    ATACAGAAAC AGATGGAGGC ATTCAGAGAT GGCTTCAATA GAGTCTTCCC AATGGAGAAG
    CTGAGTTCTT TCAGCCATGA AGAAGTTCAG ATGATTCTTT GTGGAAATCA GTCACCTTCC
    TGGGCAGCAG AGGATATCAT CAATTATACA GAACCTAAAC TGGGATATAC ACGGGACAGC
    CCTGGATTCC TTCGATTTGT GAGAGTTCTG TGTGGTATGT CTTCAGATGA AAGAAAGGCT
    TTCCTCCAGT TCACCACTGG TTGTTCAACA CTCCCACCAG GAGGACTGGC AAACCTACAT
    CCCCGGCTGA CTGTTGTACG CAAGGTTGAT GCTACAGATG CCAGTTACCC ATCTGTGAAT
    ACATGTGTGC ATTACCTGAA GTTGCCTGAG TATTCTTCTG AGGAGATTAT GAGAGAGCGT
    CTGCTAGCTG CTACAATGGA GAAAGGCTTC CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143109.1
    CDS286..7887
    Translation

    Target ORF information:

    RefSeq Version XM_015287623.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCACAACG
    CAGTCATGGA GCAGCTTGGT GAAAAACAAC TGTCCAGACA AGACGACTGC TGCTGCAGGC
    TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT GTGTGTAGCG TGGCCAGTAG CAGCGACATC
    AGCTTGGGTT CGACCAAAAT GGAGCGGAGA TCAGAAAGTG TAATGGAGCA AAATATAGTT
    TCAGGCACTG ACGTCCACGA ACCAATTGTT GTTCTGTCTT CCGCTGATAA TGTACCTCAG
    GCTGAAGTAG GGTCATCTTC CAGTGCAAGC ACCAGCACCT TAACAGCGGA CATGGGAAAT
    GAAAATACAG AAAGGAAGTT AGGTCCTGAT AATTCGATAC GTACTCCTGG GGAGTCCAGT
    GCTATCTCCA TGGGAATTGT TAGTGTAAGT TCTCCTGATG TTAGTTCAGT TTCCGAACTG
    ACTAATAAAG AAGCAGCATC CCAACGACCT CTGAGCTCTT CAGCAAGTAA CAGACTTTCA
    GTTAGCTCTT TGTTGGCTGC TGGGGCGCCA ATGAGCTCCA GTGCAAGCGT TCCTAACTTA
    TCATCTCGAG AAACTTCGAG CCTGGAGTCT TTTGTACGAA GAGTGGCAAA CATAGCACGT
    ACCAATGCCA CAAACAACAT GAATTTAAGC CGAAGTAGCA GTGATAACAA TACTAATACT
    CTGGGAAGAA ACGTCATGAG TACTGCAACG TCTCCTCTTA TGGGTGCCCA AAGTTTTCCT
    AATCTGACTA CAACTGGTAC CACATCAACA GTGACCATGT CTACATCCAG TGTTACTAGC
    AGCAGCAATG TAGCTACAGC AACTACAGTT TTATCTGTTG GTCAGTCGCT TAGTAATACT
    CTAACTACTA GCCTAACATC AACATCTAGT GAGAGCGATA CAGGGCAGGA GGCAGAATAT
    TCTTTATATG ATTTTCTGGA TAGCTGTCGA GCTAGTACTC TGTTGGCAGA GTTAGATGAT
    GATGAGGATC TACCTGAACC TGATGAAGAA GATGATGAAA ATGAAGATGA TAACCAAGAA
    GATCAAGAAT ATGAGGAAGT TATGGAAGAA GAGGAGTATG AGACCAAAGG AGGTCGTAGA
    AGAACATGGG ATGATGATTA TGTATTGAAA CGACAGTTTT CTGCTCTGGT TCCTGCTTTT
    GACCCAAGAC CTGGTCGAAC AAATGTTCAA CAAACAACTG ATTTAGAAAT CCCTCCACCG
    GGTACACCTC ATTCAGAACT CTTGGAAGAG GTTGAGTGCA TGCCTTCACC ACGCTTAGCA
    CTTACGTTGA AAGTTTCAGG CCTTGGAACA ACACGAGAAG TAGAACTGCC CCTAACAAAT
    TTTCGATTCA CAATATTTTA CTACGTACAG AAATTGTTAC AGCTTTCCTG TAATGGCAGT
    GTGAAATCTG ACAAACTTAG ACGTATTTGG GAGCCAACAT ACACAATCAT GTACAGAGAA
    ATGAAGGATT CTGATAAAGA AAAAGAAAGT GGAAAAATGG GTTGCTGGTC AGTAGAGCAT
    GTGGAGCAGT ACCTTGGCAC TGATGAATTA CCAAAGAATG ACTTGATAAC CTACCTGCAG
    AAGAATGCAG ATTCAGCTTT CCTGCGCCAC TGGAAATTAA CCGGCACTAA TAAAAGTATT
    AGGAAAAACA GAAATTGTTC CCAGCTCATA GCTGCATACA AGGATTTTTG TGAACATGGA
    TCAAAATCTG GATTAAGCCA GGGGGCCATT TCTACTCTTC AGAATTCTGA TATTCTTAGT
    TTGGCAAAAG AGCAGCCTCA AGCCAAAGCT GGCAGTGGGC AGAACTCCTG TGGAGTAGAA
    GATGTTTTGC AGTTACTTCG CATTTTGTAT ATAGTTGCTA GTGACCCTTA CACAGCGCGA
    ACTTCTCAAG AAGAAGGAGA TGAGCATCCT CAGTTTAACT TTCCACCAGA TGAATTCACA
    AGCAAAAAAA TTACTACAAA AATTCTGCAA CAGATCGAGG AACCCTTGGC ACTAGCAAGT
    GGAGCTTTGC CAGACTGGTG TGAGCAGCTA ACAAGCAAGT GTCCTTTCTT AATTCCATTT
    GAAACTAGAC AGTTATACTT CACCTGCACA GCGTTTGGAG CATCAAGAGC AATTGTGTGG
    CTACAAAACA GACGCGAAGC CACTGTTGAA AGAACAAGAA CCACAAGCAC AGTGCGACGT
    GATGATCCGG GAGAGTTCAG AGTGGGGCGC CTGAAGCATG AAAGGGTGAA AGTTCCGCGA
    GGTGAATCCC TGATGGAATG GGCAGAAAAC GTCATGCAGA TTCATGCAGA CAGAAAATCT
    GTTCTAGAGG TTGAGTTTTT AGGGGAGGAA GGAACGGGCC TGGGTCCTAC CTTGGAATTT
    TATGCATTGG TAGCAGCAGA ATTTCAGAGG ACAGATCTGG GAGCTTGGCT TTGTGATGAT
    GATTTTCCAG ATGATGAGTC TCGTCAGGTT GATATTGGTG GTGGATTAAA ACCCCCTGGT
    TACTATGTAC AGCGATCATG TGGACTCTTC ACAGCACCAT TCCCACAAGA TAGTGATGAG
    CTTGAAAGGA TAACCAAACT CTTTCATTTC CTTGGAATTT TCTTGGCTAA ATGCATTCAA
    GACAATAGAC TTGTGGATTT ACCCATTTCT AAGCCTTTTT TTAAACTCAT GTGTATGGGA
    GACATTAAAA GCAATATGAG TAAGTTGATA TATGAATCAC GGAGTGACAG GGACTTGCAC
    TGTACTGAAA GTCAGTCAGA GGCTTCTACA GAAGAAGGGC ACGATTCATT GTCAGTAGGA
    AGTTTGGAAG AAGACTCCAA ATCAGAATTT ATTCTTGATC CACCAAAACC TAAACCTCCT
    GCTTGGTTTA ATGGAATTCT AACGTGGGAA GACTTTGAAT TAATAAATCC ACATAGAGCA
    AGGTTTCTAA AAGAAATTAA GGACCTAGCT ATTAAAAGGC GTCAAATTTT AAGCAACAAA
    AGTCTGTCAG AAGATGAAAA AAATACAAAA CTACAGGAAT TAATGCTAAA GAATCCATCA
    GGTTCTGGAC CACCACTTAG CATAGAGGAT TTGGGTTTAA ATTTTCAGTT TTGCCCTTCA
    TCCAAAGTGT ATGGATTTAC AGCTGTAGAT CTCAAACCAG GTGGTGAAGA TGAGACTGTA
    ACGATGGATA ATGCAGAGGA GTATGTGGAT CTCATGTTTG ACTTCTGTAT GCATACTGGT
    ATACAGAAAC AGATGGAGGC ATTCAGAGAT GGCTTCAATA GAGTCTTCCC AATGGAGAAG
    CTGAGTTCTT TCAGCCATGA AGAAGTTCAG ATGATTCTTT GTGGAAATCA GTCACCTTCC
    TGGGCAGCAG AGGATATCAT CAATTATACA GAACCTAAAC TGGGATATAC ACGGGACAGC
    CCTGGATTCC TTCGATTTGT GAGAGTTCTG TGTGGTATGT CTTCAGATGA AAGAAAGGCT
    TTCCTCCAGT TCACCACTGG TTGTTCAACA CTCCCACCAG GAGGACTGGC AAACCTACAT
    CCCCGGCTGA CTGTTGTACG CAAGGTTGAT GCTACAGATG CCAGTTACCC ATCTGTGAAT
    ACATGTGTGC ATTACCTGAA GTTGCCTGAG TATTCTTCTG AGGAGATTAT GAGAGAGCGT
    CTGCTAGCTG CTACAATGGA GAAAGGCTTC CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36983
    Clone ID Related Accession (Same CDS sequence) XM_015287624.2 , XM_015287624.1
    Accession Version XM_015287624.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAA TTTTCTGGAT
    AGCTGTCGAG CTAGTACTCT GTTGGCAGAG TTAGATGATG ATGAGGATCT ACCTGAACCT
    GATGAAGAAG ATGATGAAAA TGAAGATGAT AACCAAGAAG ATCAAGAATA TGAGGAAGTT
    ATGGAAGAAG AGGAGTATGA GACCAAAGGA GGTCGTAGAA GAACATGGGA TGATGATTAT
    GTATTGAAAC GACAGTTTTC TGCTCTGGTT CCTGCTTTTG ACCCAAGACC TGGTCGAACA
    AATGTTCAAC AAACAACTGA TTTAGAAATC CCTCCACCGG GTACACCTCA TTCAGAACTC
    TTGGAAGAGG TTGAGTGCAT GCCTTCACCA CGCTTAGCAC TTACGTTGAA AGTTTCAGGC
    CTTGGAACAA CACGAGAAGT AGAACTGCCC CTAACAAATT TTCGATTCAC AATATTTTAC
    TACGTACAGA AATTGTTACA GCTTTCCTGT AATGGCAGTG TGAAATCTGA CAAACTTAGA
    CGTATTTGGG AGCCAACATA CACAATCATG TACAGAGAAA TGAAGGATTC TGATAAAGAA
    AAAGAAAGTG GAAAAATGGG TTGCTGGTCA GTAGAGCATG TGGAGCAGTA CCTTGGCACT
    GATGAATTAC CAAAGAATGA CTTGATAACC TACCTGCAGA AGAATGCAGA TTCAGCTTTC
    CTGCGCCACT GGAAATTAAC CGGCACTAAT AAAAGTATTA GGAAAAACAG AAATTGTTCC
    CAGCTCATAG CTGCATACAA GGATTTTTGT GAACATGGAT CAAAATCTGG ATTAAGCCAG
    GGGGCCATTT CTACTCTTCA GAATTCTGAT ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA
    GCCAAAGCTG GCAGTGGGCA GAACTCCTGT GGAGTAGAAG ATGTTTTGCA GTTACTTCGC
    ATTTTGTATA TAGTTGCTAG TGACCCTTAC ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT
    GAGCATCCTC AGTTTAACTT TCCACCAGAT GAATTCACAA GCAAAAAAAT TACTACAAAA
    ATTCTGCAAC AGATCGAGGA ACCCTTGGCA CTAGCAAGTG GAGCTTTGCC AGACTGGTGT
    GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA ATTCCATTTG AAACTAGACA GTTATACTTC
    ACCTGCACAG CGTTTGGAGC ATCAAGAGCA ATTGTGTGGC TACAAAACAG ACGCGAAGCC
    ACTGTTGAAA GAACAAGAAC CACAAGCACA GTGCGACGTG ATGATCCGGG AGAGTTCAGA
    GTGGGGCGCC TGAAGCATGA AAGGGTGAAA GTTCCGCGAG GTGAATCCCT GATGGAATGG
    GCAGAAAACG TCATGCAGAT TCATGCAGAC AGAAAATCTG TTCTAGAGGT TGAGTTTTTA
    GGGGAGGAAG GAACGGGCCT GGGTCCTACC TTGGAATTTT ATGCATTGGT AGCAGCAGAA
    TTTCAGAGGA CAGATCTGGG AGCTTGGCTT TGTGATGATG ATTTTCCAGA TGATGAGTCT
    CGTCAGGTTG ATATTGGTGG TGGATTAAAA CCCCCTGGTT ACTATGTACA GCGATCATGT
    GGACTCTTCA CAGCACCATT CCCACAAGAT AGTGATGAGC TTGAAAGGAT AACCAAACTC
    TTTCATTTCC TTGGAATTTT CTTGGCTAAA TGCATTCAAG ACAATAGACT TGTGGATTTA
    CCCATTTCTA AGCCTTTTTT TAAACTCATG TGTATGGGAG ACATTAAAAG CAATATGAGT
    AAGTTGATAT ATGAATCACG GAGTGACAGG GACTTGCACT GTACTGAAAG TCAGTCAGAG
    GCTTCTACAG AAGAAGGGCA CGATTCATTG TCAGTAGGAA GTTTGGAAGA AGACTCCAAA
    TCAGAATTTA TTCTTGATCC ACCAAAACCT AAACCTCCTG CTTGGTTTAA TGGAATTCTA
    ACGTGGGAAG ACTTTGAATT AATAAATCCA CATAGAGCAA GGTTTCTAAA AGAAATTAAG
    GACCTAGCTA TTAAAAGGCG TCAAATTTTA AGCAACAAAA GTCTGTCAGA AGATGAAAAA
    AATACAAAAC TACAGGAATT AATGCTAAAG AATCCATCAG GTTCTGGACC ACCACTTAGC
    ATAGAGGATT TGGGTTTAAA TTTTCAGTTT TGCCCTTCAT CCAAAGTGTA TGGATTTACA
    GCTGTAGATC TCAAACCAGG TGGTGAAGAT GAGACTGTAA CGATGGATAA TGCAGAGGAG
    TATGTGGATC TCATGTTTGA CTTCTGTATG CATACTGGTA TACAGAAACA GATGGAGGCA
    TTCAGAGATG GCTTCAATAG AGTCTTCCCA ATGGAGAAGC TGAGTTCTTT CAGCCATGAA
    GAAGTTCAGA TGATTCTTTG TGGAAATCAG TCACCTTCCT GGGCAGCAGA GGATATCATC
    AATTATACAG AACCTAAACT GGGATATACA CGGGACAGCC CTGGATTCCT TCGATTTGTG
    AGAGTTCTGT GTGGTATGTC TTCAGATGAA AGAAAGGCTT TCCTCCAGTT CACCACTGGT
    TGTTCAACAC TCCCACCAGG AGGACTGGCA AACCTACATC CCCGGCTGAC TGTTGTACGC
    AAGGTTGATG CTACAGATGC CAGTTACCCA TCTGTGAATA CATGTGTGCA TTACCTGAAG
    TTGCCTGAGT ATTCTTCTGA GGAGATTATG AGAGAGCGTC TGCTAGCTGC TACAATGGAG
    AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143110.1
    CDS309..7829
    Translation

    Target ORF information:

    RefSeq Version XM_015287624.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287624.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAA TTTTCTGGAT
    AGCTGTCGAG CTAGTACTCT GTTGGCAGAG TTAGATGATG ATGAGGATCT ACCTGAACCT
    GATGAAGAAG ATGATGAAAA TGAAGATGAT AACCAAGAAG ATCAAGAATA TGAGGAAGTT
    ATGGAAGAAG AGGAGTATGA GACCAAAGGA GGTCGTAGAA GAACATGGGA TGATGATTAT
    GTATTGAAAC GACAGTTTTC TGCTCTGGTT CCTGCTTTTG ACCCAAGACC TGGTCGAACA
    AATGTTCAAC AAACAACTGA TTTAGAAATC CCTCCACCGG GTACACCTCA TTCAGAACTC
    TTGGAAGAGG TTGAGTGCAT GCCTTCACCA CGCTTAGCAC TTACGTTGAA AGTTTCAGGC
    CTTGGAACAA CACGAGAAGT AGAACTGCCC CTAACAAATT TTCGATTCAC AATATTTTAC
    TACGTACAGA AATTGTTACA GCTTTCCTGT AATGGCAGTG TGAAATCTGA CAAACTTAGA
    CGTATTTGGG AGCCAACATA CACAATCATG TACAGAGAAA TGAAGGATTC TGATAAAGAA
    AAAGAAAGTG GAAAAATGGG TTGCTGGTCA GTAGAGCATG TGGAGCAGTA CCTTGGCACT
    GATGAATTAC CAAAGAATGA CTTGATAACC TACCTGCAGA AGAATGCAGA TTCAGCTTTC
    CTGCGCCACT GGAAATTAAC CGGCACTAAT AAAAGTATTA GGAAAAACAG AAATTGTTCC
    CAGCTCATAG CTGCATACAA GGATTTTTGT GAACATGGAT CAAAATCTGG ATTAAGCCAG
    GGGGCCATTT CTACTCTTCA GAATTCTGAT ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA
    GCCAAAGCTG GCAGTGGGCA GAACTCCTGT GGAGTAGAAG ATGTTTTGCA GTTACTTCGC
    ATTTTGTATA TAGTTGCTAG TGACCCTTAC ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT
    GAGCATCCTC AGTTTAACTT TCCACCAGAT GAATTCACAA GCAAAAAAAT TACTACAAAA
    ATTCTGCAAC AGATCGAGGA ACCCTTGGCA CTAGCAAGTG GAGCTTTGCC AGACTGGTGT
    GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA ATTCCATTTG AAACTAGACA GTTATACTTC
    ACCTGCACAG CGTTTGGAGC ATCAAGAGCA ATTGTGTGGC TACAAAACAG ACGCGAAGCC
    ACTGTTGAAA GAACAAGAAC CACAAGCACA GTGCGACGTG ATGATCCGGG AGAGTTCAGA
    GTGGGGCGCC TGAAGCATGA AAGGGTGAAA GTTCCGCGAG GTGAATCCCT GATGGAATGG
    GCAGAAAACG TCATGCAGAT TCATGCAGAC AGAAAATCTG TTCTAGAGGT TGAGTTTTTA
    GGGGAGGAAG GAACGGGCCT GGGTCCTACC TTGGAATTTT ATGCATTGGT AGCAGCAGAA
    TTTCAGAGGA CAGATCTGGG AGCTTGGCTT TGTGATGATG ATTTTCCAGA TGATGAGTCT
    CGTCAGGTTG ATATTGGTGG TGGATTAAAA CCCCCTGGTT ACTATGTACA GCGATCATGT
    GGACTCTTCA CAGCACCATT CCCACAAGAT AGTGATGAGC TTGAAAGGAT AACCAAACTC
    TTTCATTTCC TTGGAATTTT CTTGGCTAAA TGCATTCAAG ACAATAGACT TGTGGATTTA
    CCCATTTCTA AGCCTTTTTT TAAACTCATG TGTATGGGAG ACATTAAAAG CAATATGAGT
    AAGTTGATAT ATGAATCACG GAGTGACAGG GACTTGCACT GTACTGAAAG TCAGTCAGAG
    GCTTCTACAG AAGAAGGGCA CGATTCATTG TCAGTAGGAA GTTTGGAAGA AGACTCCAAA
    TCAGAATTTA TTCTTGATCC ACCAAAACCT AAACCTCCTG CTTGGTTTAA TGGAATTCTA
    ACGTGGGAAG ACTTTGAATT AATAAATCCA CATAGAGCAA GGTTTCTAAA AGAAATTAAG
    GACCTAGCTA TTAAAAGGCG TCAAATTTTA AGCAACAAAA GTCTGTCAGA AGATGAAAAA
    AATACAAAAC TACAGGAATT AATGCTAAAG AATCCATCAG GTTCTGGACC ACCACTTAGC
    ATAGAGGATT TGGGTTTAAA TTTTCAGTTT TGCCCTTCAT CCAAAGTGTA TGGATTTACA
    GCTGTAGATC TCAAACCAGG TGGTGAAGAT GAGACTGTAA CGATGGATAA TGCAGAGGAG
    TATGTGGATC TCATGTTTGA CTTCTGTATG CATACTGGTA TACAGAAACA GATGGAGGCA
    TTCAGAGATG GCTTCAATAG AGTCTTCCCA ATGGAGAAGC TGAGTTCTTT CAGCCATGAA
    GAAGTTCAGA TGATTCTTTG TGGAAATCAG TCACCTTCCT GGGCAGCAGA GGATATCATC
    AATTATACAG AACCTAAACT GGGATATACA CGGGACAGCC CTGGATTCCT TCGATTTGTG
    AGAGTTCTGT GTGGTATGTC TTCAGATGAA AGAAAGGCTT TCCTCCAGTT CACCACTGGT
    TGTTCAACAC TCCCACCAGG AGGACTGGCA AACCTACATC CCCGGCTGAC TGTTGTACGC
    AAGGTTGATG CTACAGATGC CAGTTACCCA TCTGTGAATA CATGTGTGCA TTACCTGAAG
    TTGCCTGAGT ATTCTTCTGA GGAGATTATG AGAGAGCGTC TGCTAGCTGC TACAATGGAG
    AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36983
    Clone ID Related Accession (Same CDS sequence) XM_015287624.2 , XM_015287624.1
    Accession Version XM_015287624.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAA TTTTCTGGAT
    AGCTGTCGAG CTAGTACTCT GTTGGCAGAG TTAGATGATG ATGAGGATCT ACCTGAACCT
    GATGAAGAAG ATGATGAAAA TGAAGATGAT AACCAAGAAG ATCAAGAATA TGAGGAAGTT
    ATGGAAGAAG AGGAGTATGA GACCAAAGGA GGTCGTAGAA GAACATGGGA TGATGATTAT
    GTATTGAAAC GACAGTTTTC TGCTCTGGTT CCTGCTTTTG ACCCAAGACC TGGTCGAACA
    AATGTTCAAC AAACAACTGA TTTAGAAATC CCTCCACCGG GTACACCTCA TTCAGAACTC
    TTGGAAGAGG TTGAGTGCAT GCCTTCACCA CGCTTAGCAC TTACGTTGAA AGTTTCAGGC
    CTTGGAACAA CACGAGAAGT AGAACTGCCC CTAACAAATT TTCGATTCAC AATATTTTAC
    TACGTACAGA AATTGTTACA GCTTTCCTGT AATGGCAGTG TGAAATCTGA CAAACTTAGA
    CGTATTTGGG AGCCAACATA CACAATCATG TACAGAGAAA TGAAGGATTC TGATAAAGAA
    AAAGAAAGTG GAAAAATGGG TTGCTGGTCA GTAGAGCATG TGGAGCAGTA CCTTGGCACT
    GATGAATTAC CAAAGAATGA CTTGATAACC TACCTGCAGA AGAATGCAGA TTCAGCTTTC
    CTGCGCCACT GGAAATTAAC CGGCACTAAT AAAAGTATTA GGAAAAACAG AAATTGTTCC
    CAGCTCATAG CTGCATACAA GGATTTTTGT GAACATGGAT CAAAATCTGG ATTAAGCCAG
    GGGGCCATTT CTACTCTTCA GAATTCTGAT ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA
    GCCAAAGCTG GCAGTGGGCA GAACTCCTGT GGAGTAGAAG ATGTTTTGCA GTTACTTCGC
    ATTTTGTATA TAGTTGCTAG TGACCCTTAC ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT
    GAGCATCCTC AGTTTAACTT TCCACCAGAT GAATTCACAA GCAAAAAAAT TACTACAAAA
    ATTCTGCAAC AGATCGAGGA ACCCTTGGCA CTAGCAAGTG GAGCTTTGCC AGACTGGTGT
    GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA ATTCCATTTG AAACTAGACA GTTATACTTC
    ACCTGCACAG CGTTTGGAGC ATCAAGAGCA ATTGTGTGGC TACAAAACAG ACGCGAAGCC
    ACTGTTGAAA GAACAAGAAC CACAAGCACA GTGCGACGTG ATGATCCGGG AGAGTTCAGA
    GTGGGGCGCC TGAAGCATGA AAGGGTGAAA GTTCCGCGAG GTGAATCCCT GATGGAATGG
    GCAGAAAACG TCATGCAGAT TCATGCAGAC AGAAAATCTG TTCTAGAGGT TGAGTTTTTA
    GGGGAGGAAG GAACGGGCCT GGGTCCTACC TTGGAATTTT ATGCATTGGT AGCAGCAGAA
    TTTCAGAGGA CAGATCTGGG AGCTTGGCTT TGTGATGATG ATTTTCCAGA TGATGAGTCT
    CGTCAGGTTG ATATTGGTGG TGGATTAAAA CCCCCTGGTT ACTATGTACA GCGATCATGT
    GGACTCTTCA CAGCACCATT CCCACAAGAT AGTGATGAGC TTGAAAGGAT AACCAAACTC
    TTTCATTTCC TTGGAATTTT CTTGGCTAAA TGCATTCAAG ACAATAGACT TGTGGATTTA
    CCCATTTCTA AGCCTTTTTT TAAACTCATG TGTATGGGAG ACATTAAAAG CAATATGAGT
    AAGTTGATAT ATGAATCACG GAGTGACAGG GACTTGCACT GTACTGAAAG TCAGTCAGAG
    GCTTCTACAG AAGAAGGGCA CGATTCATTG TCAGTAGGAA GTTTGGAAGA AGACTCCAAA
    TCAGAATTTA TTCTTGATCC ACCAAAACCT AAACCTCCTG CTTGGTTTAA TGGAATTCTA
    ACGTGGGAAG ACTTTGAATT AATAAATCCA CATAGAGCAA GGTTTCTAAA AGAAATTAAG
    GACCTAGCTA TTAAAAGGCG TCAAATTTTA AGCAACAAAA GTCTGTCAGA AGATGAAAAA
    AATACAAAAC TACAGGAATT AATGCTAAAG AATCCATCAG GTTCTGGACC ACCACTTAGC
    ATAGAGGATT TGGGTTTAAA TTTTCAGTTT TGCCCTTCAT CCAAAGTGTA TGGATTTACA
    GCTGTAGATC TCAAACCAGG TGGTGAAGAT GAGACTGTAA CGATGGATAA TGCAGAGGAG
    TATGTGGATC TCATGTTTGA CTTCTGTATG CATACTGGTA TACAGAAACA GATGGAGGCA
    TTCAGAGATG GCTTCAATAG AGTCTTCCCA ATGGAGAAGC TGAGTTCTTT CAGCCATGAA
    GAAGTTCAGA TGATTCTTTG TGGAAATCAG TCACCTTCCT GGGCAGCAGA GGATATCATC
    AATTATACAG AACCTAAACT GGGATATACA CGGGACAGCC CTGGATTCCT TCGATTTGTG
    AGAGTTCTGT GTGGTATGTC TTCAGATGAA AGAAAGGCTT TCCTCCAGTT CACCACTGGT
    TGTTCAACAC TCCCACCAGG AGGACTGGCA AACCTACATC CCCGGCTGAC TGTTGTACGC
    AAGGTTGATG CTACAGATGC CAGTTACCCA TCTGTGAATA CATGTGTGCA TTACCTGAAG
    TTGCCTGAGT ATTCTTCTGA GGAGATTATG AGAGAGCGTC TGCTAGCTGC TACAATGGAG
    AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015143110.1
    CDS286..7806
    Translation

    Target ORF information:

    RefSeq Version XM_015287624.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGTA AGTATGTAGG ATCAGAATTT
    GTGACTGGTT GGACTGGCAA AAGAGGAAGG AAGCTAAAAT CTAAACTGGA GAAGACAAAG
    CAGAAGGTAC GCACTATGGC AAGAGATTTA TACGATGATC ATTTTAAAGC TGTTGAAAGC
    ATGCCTCGAG GAGTAGTTGT AACACTGAGG AACATAGCAA CACAGTTAGA GTCTGCGTGG
    GAACTTCATA CAAACAGACA GTGTATTGAG GGAGAAAACA CTTGGAGAGA TTTAATGAAG
    ACTGCTTTGG AAAATTTAAT TGTGCTTTTG AAGGATGAGA ATACCATTTC TCCATACGAA
    ATGTGCAGTA GTGGATTAGT TCAATCATTG CTTACAGTTT TAAATAACAA TGTTGATTTA
    GATATGAAAC AAGACTGTAG TCAACTAGTT GAAAGAATAA ATGTATTCAA AACAGCATTC
    AGCGAAAATG AAGATGATGA AAGTCGACCA GCAGTTGCAT TAATTCGGAA ATTAATAGCA
    GTGTTAGAAT CTATTGAACG TCTACCTCTT CACTTGTATG ATACACCAGG ATCTACTTAT
    AATCTTCAGA TATTGACAAG ACGTTTGAGG TTTCGTTTGG AACGTGCTTC AGGGGAGACA
    TCCTTGATAG ATCGAACTGG CAGAATGCTA AAGATGGAGC CATTGGCAAC TGTTGAATCT
    CTAGAACAGT ATCTTCTGAA AATGGTTGCA AAGCAATGGT ACGACTTTGA TAGGGCTTCA
    TTTGTTTTTG TACGAAAACT GCGTGAAGGC CAAACATTTG TATTCAGGCA TCAACATGAT
    TTTGATGAAA ATGGAATTAT TTACTGGATT GGGACAAATG CGAAAACTGC ATATGAGTGG
    GTAAATCCAG CAGCCTATGG GTTAGTAGTG GTTACATCCT CAGAAGGAAG AAATCTGCCT
    TATGGCCGTT TAGAAGACAT CCTAAGCCGT GACAGTTCAG CTCTCAATTG TCATACTAAT
    GATGATAAGA ATGCTTGGTT TGCCATAGAT CTTGGCCTTT GGGTTATACC ATCAGCTTAC
    ACGTTACGCC ATGCTCGAGG TTATGGACGA TCTGCTCTCA GAAATTGGGT TTTCCAGGTA
    TCTAAGGATG GACAGAACTG GACTACTTTG TATACTCATG TTGATGATTG TAGTCTCAAC
    GAACCAGGGT CTACAGCAAC ATGGCCTTTA GATCCTCCAA AGGATGAGAA ACAAGGTTGG
    AGGCATGTTA GAATAAAACA GATGGGAAAG AATGCCAGTG GGCAAACACA CTACCTCTCT
    CTGTCTGGAT TTGAACTTTA TGGCACTGTG AATGGAGTAT GTGAAGACCA GTTGGGAAAA
    GCTGCTAAAG AAGCTGAAGC AAATCTGCGA AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT
    TTGAAATACA TGGTTCCAGG AGCTCGGGTA ATCAGAGGAA TTGATTGGAA GTGGAGAGAT
    CAGGATGGAA GCCCACAGGG TGAAGGCACT GTTACAGGAG AATTGCACAA TGGCTGGATT
    GATGTCACCT GGGATGCTGG TGGCTCTAAC TCTTACCGTA TGGGCGCAGA AGGAAAGTTT
    GACCTCAAGC TTGCACCAGG GTACGACCCT GATTCAGCGG CATCACCCAA ACCTGTTTCA
    TCCACTGTTT CAGGCACAAC GCAGTCATGG AGCAGCTTGG TGAAAAACAA CTGTCCAGAC
    AAGACGACTG CTGCTGCAGG CTCCTCAAGT AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC
    GTGGCCAGTA GCAGCGACAT CAGCTTGGGT TCGACCAAAA TGGAGCGGAG ATCAGAAAGT
    GTAATGGAGC AAAATATAGT TTCAGGCACT GACGTCCACG AACCAATTGT TGTTCTGTCT
    TCCGCTGATA ATGTACCTCA GGCTGAAGTA GGGTCATCTT CCAGTGCAAG CACCAGCACC
    TTAACAGCGG ACATGGGAAA TGAAAATACA GAAAGGAAGT TAGGTCCTGA TAATTCGATA
    CGTACTCCTG GGGAGTCCAG TGCTATCTCC ATGGGAATTG TTAGTGTAAG TTCTCCTGAT
    GTTAGTTCAG TTTCCGAACT GACTAATAAA GAAGCAGCAT CCCAACGACC TCTGAGCTCT
    TCAGCAAGTA ACAGACTTTC AGTTAGCTCT TTGTTGGCTG CTGGGGCGCC AATGAGCTCC
    AGTGCAAGCG TTCCTAACTT ATCATCTCGA GAAACTTCGA GCCTGGAGTC TTTTGTACGA
    AGAGTGGCAA ACATAGCACG TACCAATGCC ACAAACAACA TGAATTTAAG CCGAAGTAGC
    AGTGATAACA ATACTAATAC TCTGGGAAGA AACGTCATGA GTACTGCAAA TTTTCTGGAT
    AGCTGTCGAG CTAGTACTCT GTTGGCAGAG TTAGATGATG ATGAGGATCT ACCTGAACCT
    GATGAAGAAG ATGATGAAAA TGAAGATGAT AACCAAGAAG ATCAAGAATA TGAGGAAGTT
    ATGGAAGAAG AGGAGTATGA GACCAAAGGA GGTCGTAGAA GAACATGGGA TGATGATTAT
    GTATTGAAAC GACAGTTTTC TGCTCTGGTT CCTGCTTTTG ACCCAAGACC TGGTCGAACA
    AATGTTCAAC AAACAACTGA TTTAGAAATC CCTCCACCGG GTACACCTCA TTCAGAACTC
    TTGGAAGAGG TTGAGTGCAT GCCTTCACCA CGCTTAGCAC TTACGTTGAA AGTTTCAGGC
    CTTGGAACAA CACGAGAAGT AGAACTGCCC CTAACAAATT TTCGATTCAC AATATTTTAC
    TACGTACAGA AATTGTTACA GCTTTCCTGT AATGGCAGTG TGAAATCTGA CAAACTTAGA
    CGTATTTGGG AGCCAACATA CACAATCATG TACAGAGAAA TGAAGGATTC TGATAAAGAA
    AAAGAAAGTG GAAAAATGGG TTGCTGGTCA GTAGAGCATG TGGAGCAGTA CCTTGGCACT
    GATGAATTAC CAAAGAATGA CTTGATAACC TACCTGCAGA AGAATGCAGA TTCAGCTTTC
    CTGCGCCACT GGAAATTAAC CGGCACTAAT AAAAGTATTA GGAAAAACAG AAATTGTTCC
    CAGCTCATAG CTGCATACAA GGATTTTTGT GAACATGGAT CAAAATCTGG ATTAAGCCAG
    GGGGCCATTT CTACTCTTCA GAATTCTGAT ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA
    GCCAAAGCTG GCAGTGGGCA GAACTCCTGT GGAGTAGAAG ATGTTTTGCA GTTACTTCGC
    ATTTTGTATA TAGTTGCTAG TGACCCTTAC ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT
    GAGCATCCTC AGTTTAACTT TCCACCAGAT GAATTCACAA GCAAAAAAAT TACTACAAAA
    ATTCTGCAAC AGATCGAGGA ACCCTTGGCA CTAGCAAGTG GAGCTTTGCC AGACTGGTGT
    GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA ATTCCATTTG AAACTAGACA GTTATACTTC
    ACCTGCACAG CGTTTGGAGC ATCAAGAGCA ATTGTGTGGC TACAAAACAG ACGCGAAGCC
    ACTGTTGAAA GAACAAGAAC CACAAGCACA GTGCGACGTG ATGATCCGGG AGAGTTCAGA
    GTGGGGCGCC TGAAGCATGA AAGGGTGAAA GTTCCGCGAG GTGAATCCCT GATGGAATGG
    GCAGAAAACG TCATGCAGAT TCATGCAGAC AGAAAATCTG TTCTAGAGGT TGAGTTTTTA
    GGGGAGGAAG GAACGGGCCT GGGTCCTACC TTGGAATTTT ATGCATTGGT AGCAGCAGAA
    TTTCAGAGGA CAGATCTGGG AGCTTGGCTT TGTGATGATG ATTTTCCAGA TGATGAGTCT
    CGTCAGGTTG ATATTGGTGG TGGATTAAAA CCCCCTGGTT ACTATGTACA GCGATCATGT
    GGACTCTTCA CAGCACCATT CCCACAAGAT AGTGATGAGC TTGAAAGGAT AACCAAACTC
    TTTCATTTCC TTGGAATTTT CTTGGCTAAA TGCATTCAAG ACAATAGACT TGTGGATTTA
    CCCATTTCTA AGCCTTTTTT TAAACTCATG TGTATGGGAG ACATTAAAAG CAATATGAGT
    AAGTTGATAT ATGAATCACG GAGTGACAGG GACTTGCACT GTACTGAAAG TCAGTCAGAG
    GCTTCTACAG AAGAAGGGCA CGATTCATTG TCAGTAGGAA GTTTGGAAGA AGACTCCAAA
    TCAGAATTTA TTCTTGATCC ACCAAAACCT AAACCTCCTG CTTGGTTTAA TGGAATTCTA
    ACGTGGGAAG ACTTTGAATT AATAAATCCA CATAGAGCAA GGTTTCTAAA AGAAATTAAG
    GACCTAGCTA TTAAAAGGCG TCAAATTTTA AGCAACAAAA GTCTGTCAGA AGATGAAAAA
    AATACAAAAC TACAGGAATT AATGCTAAAG AATCCATCAG GTTCTGGACC ACCACTTAGC
    ATAGAGGATT TGGGTTTAAA TTTTCAGTTT TGCCCTTCAT CCAAAGTGTA TGGATTTACA
    GCTGTAGATC TCAAACCAGG TGGTGAAGAT GAGACTGTAA CGATGGATAA TGCAGAGGAG
    TATGTGGATC TCATGTTTGA CTTCTGTATG CATACTGGTA TACAGAAACA GATGGAGGCA
    TTCAGAGATG GCTTCAATAG AGTCTTCCCA ATGGAGAAGC TGAGTTCTTT CAGCCATGAA
    GAAGTTCAGA TGATTCTTTG TGGAAATCAG TCACCTTCCT GGGCAGCAGA GGATATCATC
    AATTATACAG AACCTAAACT GGGATATACA CGGGACAGCC CTGGATTCCT TCGATTTGTG
    AGAGTTCTGT GTGGTATGTC TTCAGATGAA AGAAAGGCTT TCCTCCAGTT CACCACTGGT
    TGTTCAACAC TCCCACCAGG AGGACTGGCA AACCTACATC CCCGGCTGAC TGTTGTACGC
    AAGGTTGATG CTACAGATGC CAGTTACCCA TCTGTGAATA CATGTGTGCA TTACCTGAAG
    TTGCCTGAGT ATTCTTCTGA GGAGATTATG AGAGAGCGTC TGCTAGCTGC TACAATGGAG
    AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09382
    Clone ID Related Accession (Same CDS sequence) XM_004941742.2 , XM_004941742.3
    Accession Version XM_004941742.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004941742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGAT CAGAATTTGT GACTGGTTGG
    ACTGGCAAAA GAGGAAGGAA GCTAAAATCT AAACTGGAGA AGACAAAGCA GAAGGTACGC
    ACTATGGCAA GAGATTTATA CGATGATCAT TTTAAAGCTG TTGAAAGCAT GCCTCGAGGA
    GTAGTTGTAA CACTGAGGAA CATAGCAACA CAGTTAGAGT CTGCGTGGGA ACTTCATACA
    AACAGACAGT GTATTGAGGG AGAAAACACT TGGAGAGATT TAATGAAGAC TGCTTTGGAA
    AATTTAATTG TGCTTTTGAA GGATGAGAAT ACCATTTCTC CATACGAAAT GTGCAGTAGT
    GGATTAGTTC AATCATTGCT TACAGTTTTA AATAACAATG TTGATTTAGA TATGAAACAA
    GACTGTAGTC AACTAGTTGA AAGAATAAAT GTATTCAAAA CAGCATTCAG CGAAAATGAA
    GATGATGAAA GTCGACCAGC AGTTGCATTA ATTCGGAAAT TAATAGCAGT GTTAGAATCT
    ATTGAACGTC TACCTCTTCA CTTGTATGAT ACACCAGGAT CTACTTATAA TCTTCAGATA
    TTGACAAGAC GTTTGAGGTT TCGTTTGGAA CGTGCTTCAG GGGAGACATC CTTGATAGAT
    CGAACTGGCA GAATGCTAAA GATGGAGCCA TTGGCAACTG TTGAATCTCT AGAACAGTAT
    CTTCTGAAAA TGGTTGCAAA GCAATGGTAC GACTTTGATA GGGCTTCATT TGTTTTTGTA
    CGAAAACTGC GTGAAGGCCA AACATTTGTA TTCAGGCATC AACATGATTT TGATGAAAAT
    GGAATTATTT ACTGGATTGG GACAAATGCG AAAACTGCAT ATGAGTGGGT AAATCCAGCA
    GCCTATGGGT TAGTAGTGGT TACATCCTCA GAAGGAAGAA ATCTGCCTTA TGGCCGTTTA
    GAAGACATCC TAAGCCGTGA CAGTTCAGCT CTCAATTGTC ATACTAATGA TGATAAGAAT
    GCTTGGTTTG CCATAGATCT TGGCCTTTGG GTTATACCAT CAGCTTACAC GTTACGCCAT
    GCTCGAGGTT ATGGACGATC TGCTCTCAGA AATTGGGTTT TCCAGGTATC TAAGGATGGA
    CAGAACTGGA CTACTTTGTA TACTCATGTT GATGATTGTA GTCTCAACGA ACCAGGGTCT
    ACAGCAACAT GGCCTTTAGA TCCTCCAAAG GATGAGAAAC AAGGTTGGAG GCATGTTAGA
    ATAAAACAGA TGGGAAAGAA TGCCAGTGGG CAAACACACT ACCTCTCTCT GTCTGGATTT
    GAACTTTATG GCACTGTGAA TGGAGTATGT GAAGACCAGT TGGGAAAAGC TGCTAAAGAA
    GCTGAAGCAA ATCTGCGAAG GCAGCGACGT CTGGTCCGGT CTCAAGTTTT GAAATACATG
    GTTCCAGGAG CTCGGGTAAT CAGAGGAATT GATTGGAAGT GGAGAGATCA GGATGGAAGC
    CCACAGGGTG AAGGCACTGT TACAGGAGAA TTGCACAATG GCTGGATTGA TGTCACCTGG
    GATGCTGGTG GCTCTAACTC TTACCGTATG GGCGCAGAAG GAAAGTTTGA CCTCAAGCTT
    GCACCAGGGT ACGACCCTGA TTCAGCGGCA TCACCCAAAC CTGTTTCATC CACTGTTTCA
    GGCACAACGC AGTCATGGAG CAGCTTGGTG AAAAACAACT GTCCAGACAA GACGACTGCT
    GCTGCAGGCT CCTCAAGTAG AAAAGGAAGC AGCAGTTCTG TGTGTAGCGT GGCCAGTAGC
    AGCGACATCA GCTTGGGTTC GACCAAAATG GAGCGGAGAT CAGAAAGTGT AATGGAGCAA
    AATATAGTTT CAGGCACTGA CGTCCACGAA CCAATTGTTG TTCTGTCTTC CGCTGATAAT
    GTACCTCAGG CTGAAGTAGG GTCATCTTCC AGTGCAAGCA CCAGCACCTT AACAGCGGAC
    ATGGGAAATG AAAATACAGA AAGGAAGTTA GGTCCTGATA ATTCGATACG TACTCCTGGG
    GAGTCCAGTG CTATCTCCAT GGGAATTGTT AGTGTAAGTT CTCCTGATGT TAGTTCAGTT
    TCCGAACTGA CTAATAAAGA AGCAGCATCC CAACGACCTC TGAGCTCTTC AGCAAGTAAC
    AGACTTTCAG TTAGCTCTTT GTTGGCTGCT GGGGCGCCAA TGAGCTCCAG TGCAAGCGTT
    CCTAACTTAT CATCTCGAGA AACTTCGAGC CTGGAGTCTT TTGTACGAAG AGTGGCAAAC
    ATAGCACGTA CCAATGCCAC AAACAACATG AATTTAAGCC GAAGTAGCAG TGATAACAAT
    ACTAATACTC TGGGAAGAAA CGTCATGAGT ACTGCAACGT CTCCTCTTAT GGGTGCCCAA
    AGTTTTCCTA ATCTGACTAC AACTGGTACC ACATCAACAG TGACCATGTC TACATCCAGT
    GTTACTAGCA GCAGCAATGT AGCTACAGCA ACTACAGTTT TATCTGTTGG TCAGTCGCTT
    AGTAATACTC TAACTACTAG CCTAACATCA ACATCTAGTG AGAGCGATAC AGGGCAGGAG
    GCAGAATATT CTTTATATGA TTTTCTGGAT AGCTGTCGAG CTAGTACTCT GTTGGCAGAG
    TTAGATGATG ATGAGGATCT ACCTGAACCT GATGAAGAAG ATGATGAAAA TGAAGATGAT
    AACCAAGAAG ATCAAGAATA TGAGGAAGTT ATGGAAGAAG AGGAGTATGA GACCAAAGGA
    GGTCGTAGAA GAACATGGGA TGATGATTAT GTATTGAAAC GACAGTTTTC TGCTCTGGTT
    CCTGCTTTTG ACCCAAGACC TGGTCGAACA AATGTTCAAC AAACAACTGA TTTAGAAATC
    CCTCCACCGG GTACACCTCA TTCAGAACTC TTGGAAGAGG TTGAGTGCAT GCCTTCACCA
    CGCTTAGCAC TTACGTTGAA AGTTTCAGGC CTTGGAACAA CACGAGAAGT AGAACTGCCC
    CTAACAAATT TTCGATTCAC AATATTTTAC TACGTACAGA AATTGTTACA GCTTTCCTGT
    AATGGCAGTG TGAAATCTGA CAAACTTAGA CGTATTTGGG AGCCAACATA CACAATCATG
    TACAGAGAAA TGAAGGATTC TGATAAAGAA AAAGAAAGTG GAAAAATGGG TTGCTGGTCA
    GTAGAGCATG TGGAGCAGTA CCTTGGCACT GATGAATTAC CAAAGAATGA CTTGATAACC
    TACCTGCAGA AGAATGCAGA TTCAGCTTTC CTGCGCCACT GGAAATTAAC CGGCACTAAT
    AAAAGTATTA GGAAAAACAG AAATTGTTCC CAGCTCATAG CTGCATACAA GGATTTTTGT
    GAACATGGAT CAAAATCTGG ATTAAGCCAG GGGGCCATTT CTACTCTTCA GAATTCTGAT
    ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA GCCAAAGCTG GCAGTGGGCA GAACTCCTGT
    GGAGTAGAAG ATGTTTTGCA GTTACTTCGC ATTTTGTATA TAGTTGCTAG TGACCCTTAC
    ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT GAGCATCCTC AGTTTAACTT TCCACCAGAT
    GAATTCACAA GCAAAAAAAT TACTACAAAA ATTCTGCAAC AGATCGAGGA ACCCTTGGCA
    CTAGCAAGTG GAGCTTTGCC AGACTGGTGT GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA
    ATTCCATTTG AAACTAGACA GTTATACTTC ACCTGCACAG CGTTTGGAGC ATCAAGAGCA
    ATTGTGTGGC TACAAAACAG ACGCGAAGCC ACTGTTGAAA GAACAAGAAC CACAAGCACA
    GTGCGACGTG ATGATCCGGG AGAGTTCAGA GTGGGGCGCC TGAAGCATGA AAGGGTGAAA
    GTTCCGCGAG GTGAATCCCT GATGGAATGG GCAGAAAACG TCATGCAGAT TCATGCAGAC
    AGAAAATCTG TTCTAGAGGT TGAGTTTTTA GGGGAGGAAG GAACGGGCCT GGGTCCTACC
    TTGGAATTTT ATGCATTGGT AGCAGCAGAA TTTCAGAGGA CAGATCTGGG AGCTTGGCTT
    TGTGATGATG ATTTTCCAGA TGATGAGTCT CGTCAGGTTG ATATTGGTGG TGGATTAAAA
    CCCCCTGGTT ACTATGTACA GCGATCATGT GGACTCTTCA CAGCACCATT CCCACAAGAT
    AGTGATGAGC TTGAAAGGAT AACCAAACTC TTTCATTTCC TTGGAATTTT CTTGGCTAAA
    TGCATTCAAG ACAATAGACT TGTGGATTTA CCCATTTCTA AGCCTTTTTT TAAACTCATG
    TGTATGGGAG ACATTAAAAG CAATATGAGT AAGTTGATAT ATGAATCACG GAGTGACAGG
    GACTTGCACT GTACTGAAAG TCAGTCAGAG GCTTCTACAG AAGAAGGGCA CGATTCATTG
    TCAGTAGGAA GTTTGGAAGA AGACTCCAAA TCAGAATTTA TTCTTGATCC ACCAAAACCT
    AAACCTCCTG CTTGGTTTAA TGGAATTCTA ACGTGGGAAG ACTTTGAATT AATAAATCCA
    CATAGAGCAA GGTTTCTAAA AGAAATTAAG GACCTAGCTA TTAAAAGGCG TCAAATTTTA
    AGCAACAAAA GTCTGTCAGA AGATGAAAAA AATACAAAAC TACAGGAATT AATGCTAAAG
    AATCCATCAG GTTCTGGACC ACCACTTAGC ATAGAGGATT TGGGTTTAAA TTTTCAGTTT
    TGCCCTTCAT CCAAAGTGTA TGGATTTACA GCTGTAGATC TCAAACCAGG TGGTGAAGAT
    GAGACTGTAA CGATGGATAA TGCAGAGGAG TATGTGGATC TCATGTTTGA CTTCTGTATG
    CATACTGGTA TACAGAAACA GATGGAGGCA TTCAGAGATG GCTTCAATAG AGTCTTCCCA
    ATGGAGAAGC TGAGTTCTTT CAGCCATGAA GAAGTTCAGA TGATTCTTTG TGGAAATCAG
    TCACCTTCCT GGGCAGCAGA GGATATCATC AATTATACAG AACCTAAACT GGGATATACA
    CGGGACAGCC CTGGATTCCT TCGATTTGTG AGAGTTCTGT GTGGTATGTC TTCAGATGAA
    AGAAAGGCTT TCCTCCAGTT CACCACTGGT TGTTCAACAC TCCCACCAGG AGGACTGGCA
    AACCTACATC CCCGGCTGAC TGTTGTACGC AAGGTTGATG CTACAGATGC CAGTTACCCA
    TCTGTGAATA CATGTGTGCA TTACCTGAAG TTGCCTGAGT ATTCTTCTGA GGAGATTATG
    AGAGAGCGTC TGCTAGCTGC TACAATGGAG AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941799.1
    CDS286..8016
    Translation

    Target ORF information:

    RefSeq Version XM_004941742.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941742.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGAT CAGAATTTGT GACTGGTTGG
    ACTGGCAAAA GAGGAAGGAA GCTAAAATCT AAACTGGAGA AGACAAAGCA GAAGGTACGC
    ACTATGGCAA GAGATTTATA CGATGATCAT TTTAAAGCTG TTGAAAGCAT GCCTCGAGGA
    GTAGTTGTAA CACTGAGGAA CATAGCAACA CAGTTAGAGT CTGCGTGGGA ACTTCATACA
    AACAGACAGT GTATTGAGGG AGAAAACACT TGGAGAGATT TAATGAAGAC TGCTTTGGAA
    AATTTAATTG TGCTTTTGAA GGATGAGAAT ACCATTTCTC CATACGAAAT GTGCAGTAGT
    GGATTAGTTC AATCATTGCT TACAGTTTTA AATAACAATG TTGATTTAGA TATGAAACAA
    GACTGTAGTC AACTAGTTGA AAGAATAAAT GTATTCAAAA CAGCATTCAG CGAAAATGAA
    GATGATGAAA GTCGACCAGC AGTTGCATTA ATTCGGAAAT TAATAGCAGT GTTAGAATCT
    ATTGAACGTC TACCTCTTCA CTTGTATGAT ACACCAGGAT CTACTTATAA TCTTCAGATA
    TTGACAAGAC GTTTGAGGTT TCGTTTGGAA CGTGCTTCAG GGGAGACATC CTTGATAGAT
    CGAACTGGCA GAATGCTAAA GATGGAGCCA TTGGCAACTG TTGAATCTCT AGAACAGTAT
    CTTCTGAAAA TGGTTGCAAA GCAATGGTAC GACTTTGATA GGGCTTCATT TGTTTTTGTA
    CGAAAACTGC GTGAAGGCCA AACATTTGTA TTCAGGCATC AACATGATTT TGATGAAAAT
    GGAATTATTT ACTGGATTGG GACAAATGCG AAAACTGCAT ATGAGTGGGT AAATCCAGCA
    GCCTATGGGT TAGTAGTGGT TACATCCTCA GAAGGAAGAA ATCTGCCTTA TGGCCGTTTA
    GAAGACATCC TAAGCCGTGA CAGTTCAGCT CTCAATTGTC ATACTAATGA TGATAAGAAT
    GCTTGGTTTG CCATAGATCT TGGCCTTTGG GTTATACCAT CAGCTTACAC GTTACGCCAT
    GCTCGAGGTT ATGGACGATC TGCTCTCAGA AATTGGGTTT TCCAGGTATC TAAGGATGGA
    CAGAACTGGA CTACTTTGTA TACTCATGTT GATGATTGTA GTCTCAACGA ACCAGGGTCT
    ACAGCAACAT GGCCTTTAGA TCCTCCAAAG GATGAGAAAC AAGGTTGGAG GCATGTTAGA
    ATAAAACAGA TGGGAAAGAA TGCCAGTGGG CAAACACACT ACCTCTCTCT GTCTGGATTT
    GAACTTTATG GCACTGTGAA TGGAGTATGT GAAGACCAGT TGGGAAAAGC TGCTAAAGAA
    GCTGAAGCAA ATCTGCGAAG GCAGCGACGT CTGGTCCGGT CTCAAGTTTT GAAATACATG
    GTTCCAGGAG CTCGGGTAAT CAGAGGAATT GATTGGAAGT GGAGAGATCA GGATGGAAGC
    CCACAGGGTG AAGGCACTGT TACAGGAGAA TTGCACAATG GCTGGATTGA TGTCACCTGG
    GATGCTGGTG GCTCTAACTC TTACCGTATG GGCGCAGAAG GAAAGTTTGA CCTCAAGCTT
    GCACCAGGGT ACGACCCTGA TTCAGCGGCA TCACCCAAAC CTGTTTCATC CACTGTTTCA
    GGCACAACGC AGTCATGGAG CAGCTTGGTG AAAAACAACT GTCCAGACAA GACGACTGCT
    GCTGCAGGCT CCTCAAGTAG AAAAGGAAGC AGCAGTTCTG TGTGTAGCGT GGCCAGTAGC
    AGCGACATCA GCTTGGGTTC GACCAAAATG GAGCGGAGAT CAGAAAGTGT AATGGAGCAA
    AATATAGTTT CAGGCACTGA CGTCCACGAA CCAATTGTTG TTCTGTCTTC CGCTGATAAT
    GTACCTCAGG CTGAAGTAGG GTCATCTTCC AGTGCAAGCA CCAGCACCTT AACAGCGGAC
    ATGGGAAATG AAAATACAGA AAGGAAGTTA GGTCCTGATA ATTCGATACG TACTCCTGGG
    GAGTCCAGTG CTATCTCCAT GGGAATTGTT AGTGTAAGTT CTCCTGATGT TAGTTCAGTT
    TCCGAACTGA CTAATAAAGA AGCAGCATCC CAACGACCTC TGAGCTCTTC AGCAAGTAAC
    AGACTTTCAG TTAGCTCTTT GTTGGCTGCT GGGGCGCCAA TGAGCTCCAG TGCAAGCGTT
    CCTAACTTAT CATCTCGAGA AACTTCGAGC CTGGAGTCTT TTGTACGAAG AGTGGCAAAC
    ATAGCACGTA CCAATGCCAC AAACAACATG AATTTAAGCC GAAGTAGCAG TGATAACAAT
    ACTAATACTC TGGGAAGAAA CGTCATGAGT ACTGCAACGT CTCCTCTTAT GGGTGCCCAA
    AGTTTTCCTA ATCTGACTAC AACTGGTACC ACATCAACAG TGACCATGTC TACATCCAGT
    GTTACTAGCA GCAGCAATGT AGCTACAGCA ACTACAGTTT TATCTGTTGG TCAGTCGCTT
    AGTAATACTC TAACTACTAG CCTAACATCA ACATCTAGTG AGAGCGATAC AGGGCAGGAG
    GCAGAATATT CTTTATATGA TTTTCTGGAT AGCTGTCGAG CTAGTACTCT GTTGGCAGAG
    TTAGATGATG ATGAGGATCT ACCTGAACCT GATGAAGAAG ATGATGAAAA TGAAGATGAT
    AACCAAGAAG ATCAAGAATA TGAGGAAGTT ATGGAAGAAG AGGAGTATGA GACCAAAGGA
    GGTCGTAGAA GAACATGGGA TGATGATTAT GTATTGAAAC GACAGTTTTC TGCTCTGGTT
    CCTGCTTTTG ACCCAAGACC TGGTCGAACA AATGTTCAAC AAACAACTGA TTTAGAAATC
    CCTCCACCGG GTACACCTCA TTCAGAACTC TTGGAAGAGG TTGAGTGCAT GCCTTCACCA
    CGCTTAGCAC TTACGTTGAA AGTTTCAGGC CTTGGAACAA CACGAGAAGT AGAACTGCCC
    CTAACAAATT TTCGATTCAC AATATTTTAC TACGTACAGA AATTGTTACA GCTTTCCTGT
    AATGGCAGTG TGAAATCTGA CAAACTTAGA CGTATTTGGG AGCCAACATA CACAATCATG
    TACAGAGAAA TGAAGGATTC TGATAAAGAA AAAGAAAGTG GAAAAATGGG TTGCTGGTCA
    GTAGAGCATG TGGAGCAGTA CCTTGGCACT GATGAATTAC CAAAGAATGA CTTGATAACC
    TACCTGCAGA AGAATGCAGA TTCAGCTTTC CTGCGCCACT GGAAATTAAC CGGCACTAAT
    AAAAGTATTA GGAAAAACAG AAATTGTTCC CAGCTCATAG CTGCATACAA GGATTTTTGT
    GAACATGGAT CAAAATCTGG ATTAAGCCAG GGGGCCATTT CTACTCTTCA GAATTCTGAT
    ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA GCCAAAGCTG GCAGTGGGCA GAACTCCTGT
    GGAGTAGAAG ATGTTTTGCA GTTACTTCGC ATTTTGTATA TAGTTGCTAG TGACCCTTAC
    ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT GAGCATCCTC AGTTTAACTT TCCACCAGAT
    GAATTCACAA GCAAAAAAAT TACTACAAAA ATTCTGCAAC AGATCGAGGA ACCCTTGGCA
    CTAGCAAGTG GAGCTTTGCC AGACTGGTGT GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA
    ATTCCATTTG AAACTAGACA GTTATACTTC ACCTGCACAG CGTTTGGAGC ATCAAGAGCA
    ATTGTGTGGC TACAAAACAG ACGCGAAGCC ACTGTTGAAA GAACAAGAAC CACAAGCACA
    GTGCGACGTG ATGATCCGGG AGAGTTCAGA GTGGGGCGCC TGAAGCATGA AAGGGTGAAA
    GTTCCGCGAG GTGAATCCCT GATGGAATGG GCAGAAAACG TCATGCAGAT TCATGCAGAC
    AGAAAATCTG TTCTAGAGGT TGAGTTTTTA GGGGAGGAAG GAACGGGCCT GGGTCCTACC
    TTGGAATTTT ATGCATTGGT AGCAGCAGAA TTTCAGAGGA CAGATCTGGG AGCTTGGCTT
    TGTGATGATG ATTTTCCAGA TGATGAGTCT CGTCAGGTTG ATATTGGTGG TGGATTAAAA
    CCCCCTGGTT ACTATGTACA GCGATCATGT GGACTCTTCA CAGCACCATT CCCACAAGAT
    AGTGATGAGC TTGAAAGGAT AACCAAACTC TTTCATTTCC TTGGAATTTT CTTGGCTAAA
    TGCATTCAAG ACAATAGACT TGTGGATTTA CCCATTTCTA AGCCTTTTTT TAAACTCATG
    TGTATGGGAG ACATTAAAAG CAATATGAGT AAGTTGATAT ATGAATCACG GAGTGACAGG
    GACTTGCACT GTACTGAAAG TCAGTCAGAG GCTTCTACAG AAGAAGGGCA CGATTCATTG
    TCAGTAGGAA GTTTGGAAGA AGACTCCAAA TCAGAATTTA TTCTTGATCC ACCAAAACCT
    AAACCTCCTG CTTGGTTTAA TGGAATTCTA ACGTGGGAAG ACTTTGAATT AATAAATCCA
    CATAGAGCAA GGTTTCTAAA AGAAATTAAG GACCTAGCTA TTAAAAGGCG TCAAATTTTA
    AGCAACAAAA GTCTGTCAGA AGATGAAAAA AATACAAAAC TACAGGAATT AATGCTAAAG
    AATCCATCAG GTTCTGGACC ACCACTTAGC ATAGAGGATT TGGGTTTAAA TTTTCAGTTT
    TGCCCTTCAT CCAAAGTGTA TGGATTTACA GCTGTAGATC TCAAACCAGG TGGTGAAGAT
    GAGACTGTAA CGATGGATAA TGCAGAGGAG TATGTGGATC TCATGTTTGA CTTCTGTATG
    CATACTGGTA TACAGAAACA GATGGAGGCA TTCAGAGATG GCTTCAATAG AGTCTTCCCA
    ATGGAGAAGC TGAGTTCTTT CAGCCATGAA GAAGTTCAGA TGATTCTTTG TGGAAATCAG
    TCACCTTCCT GGGCAGCAGA GGATATCATC AATTATACAG AACCTAAACT GGGATATACA
    CGGGACAGCC CTGGATTCCT TCGATTTGTG AGAGTTCTGT GTGGTATGTC TTCAGATGAA
    AGAAAGGCTT TCCTCCAGTT CACCACTGGT TGTTCAACAC TCCCACCAGG AGGACTGGCA
    AACCTACATC CCCGGCTGAC TGTTGTACGC AAGGTTGATG CTACAGATGC CAGTTACCCA
    TCTGTGAATA CATGTGTGCA TTACCTGAAG TTGCCTGAGT ATTCTTCTGA GGAGATTATG
    AGAGAGCGTC TGCTAGCTGC TACAATGGAG AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09382
    Clone ID Related Accession (Same CDS sequence) XM_004941742.2 , XM_004941742.3
    Accession Version XM_004941742.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004941742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGAT CAGAATTTGT GACTGGTTGG
    ACTGGCAAAA GAGGAAGGAA GCTAAAATCT AAACTGGAGA AGACAAAGCA GAAGGTACGC
    ACTATGGCAA GAGATTTATA CGATGATCAT TTTAAAGCTG TTGAAAGCAT GCCTCGAGGA
    GTAGTTGTAA CACTGAGGAA CATAGCAACA CAGTTAGAGT CTGCGTGGGA ACTTCATACA
    AACAGACAGT GTATTGAGGG AGAAAACACT TGGAGAGATT TAATGAAGAC TGCTTTGGAA
    AATTTAATTG TGCTTTTGAA GGATGAGAAT ACCATTTCTC CATACGAAAT GTGCAGTAGT
    GGATTAGTTC AATCATTGCT TACAGTTTTA AATAACAATG TTGATTTAGA TATGAAACAA
    GACTGTAGTC AACTAGTTGA AAGAATAAAT GTATTCAAAA CAGCATTCAG CGAAAATGAA
    GATGATGAAA GTCGACCAGC AGTTGCATTA ATTCGGAAAT TAATAGCAGT GTTAGAATCT
    ATTGAACGTC TACCTCTTCA CTTGTATGAT ACACCAGGAT CTACTTATAA TCTTCAGATA
    TTGACAAGAC GTTTGAGGTT TCGTTTGGAA CGTGCTTCAG GGGAGACATC CTTGATAGAT
    CGAACTGGCA GAATGCTAAA GATGGAGCCA TTGGCAACTG TTGAATCTCT AGAACAGTAT
    CTTCTGAAAA TGGTTGCAAA GCAATGGTAC GACTTTGATA GGGCTTCATT TGTTTTTGTA
    CGAAAACTGC GTGAAGGCCA AACATTTGTA TTCAGGCATC AACATGATTT TGATGAAAAT
    GGAATTATTT ACTGGATTGG GACAAATGCG AAAACTGCAT ATGAGTGGGT AAATCCAGCA
    GCCTATGGGT TAGTAGTGGT TACATCCTCA GAAGGAAGAA ATCTGCCTTA TGGCCGTTTA
    GAAGACATCC TAAGCCGTGA CAGTTCAGCT CTCAATTGTC ATACTAATGA TGATAAGAAT
    GCTTGGTTTG CCATAGATCT TGGCCTTTGG GTTATACCAT CAGCTTACAC GTTACGCCAT
    GCTCGAGGTT ATGGACGATC TGCTCTCAGA AATTGGGTTT TCCAGGTATC TAAGGATGGA
    CAGAACTGGA CTACTTTGTA TACTCATGTT GATGATTGTA GTCTCAACGA ACCAGGGTCT
    ACAGCAACAT GGCCTTTAGA TCCTCCAAAG GATGAGAAAC AAGGTTGGAG GCATGTTAGA
    ATAAAACAGA TGGGAAAGAA TGCCAGTGGG CAAACACACT ACCTCTCTCT GTCTGGATTT
    GAACTTTATG GCACTGTGAA TGGAGTATGT GAAGACCAGT TGGGAAAAGC TGCTAAAGAA
    GCTGAAGCAA ATCTGCGAAG GCAGCGACGT CTGGTCCGGT CTCAAGTTTT GAAATACATG
    GTTCCAGGAG CTCGGGTAAT CAGAGGAATT GATTGGAAGT GGAGAGATCA GGATGGAAGC
    CCACAGGGTG AAGGCACTGT TACAGGAGAA TTGCACAATG GCTGGATTGA TGTCACCTGG
    GATGCTGGTG GCTCTAACTC TTACCGTATG GGCGCAGAAG GAAAGTTTGA CCTCAAGCTT
    GCACCAGGGT ACGACCCTGA TTCAGCGGCA TCACCCAAAC CTGTTTCATC CACTGTTTCA
    GGCACAACGC AGTCATGGAG CAGCTTGGTG AAAAACAACT GTCCAGACAA GACGACTGCT
    GCTGCAGGCT CCTCAAGTAG AAAAGGAAGC AGCAGTTCTG TGTGTAGCGT GGCCAGTAGC
    AGCGACATCA GCTTGGGTTC GACCAAAATG GAGCGGAGAT CAGAAAGTGT AATGGAGCAA
    AATATAGTTT CAGGCACTGA CGTCCACGAA CCAATTGTTG TTCTGTCTTC CGCTGATAAT
    GTACCTCAGG CTGAAGTAGG GTCATCTTCC AGTGCAAGCA CCAGCACCTT AACAGCGGAC
    ATGGGAAATG AAAATACAGA AAGGAAGTTA GGTCCTGATA ATTCGATACG TACTCCTGGG
    GAGTCCAGTG CTATCTCCAT GGGAATTGTT AGTGTAAGTT CTCCTGATGT TAGTTCAGTT
    TCCGAACTGA CTAATAAAGA AGCAGCATCC CAACGACCTC TGAGCTCTTC AGCAAGTAAC
    AGACTTTCAG TTAGCTCTTT GTTGGCTGCT GGGGCGCCAA TGAGCTCCAG TGCAAGCGTT
    CCTAACTTAT CATCTCGAGA AACTTCGAGC CTGGAGTCTT TTGTACGAAG AGTGGCAAAC
    ATAGCACGTA CCAATGCCAC AAACAACATG AATTTAAGCC GAAGTAGCAG TGATAACAAT
    ACTAATACTC TGGGAAGAAA CGTCATGAGT ACTGCAACGT CTCCTCTTAT GGGTGCCCAA
    AGTTTTCCTA ATCTGACTAC AACTGGTACC ACATCAACAG TGACCATGTC TACATCCAGT
    GTTACTAGCA GCAGCAATGT AGCTACAGCA ACTACAGTTT TATCTGTTGG TCAGTCGCTT
    AGTAATACTC TAACTACTAG CCTAACATCA ACATCTAGTG AGAGCGATAC AGGGCAGGAG
    GCAGAATATT CTTTATATGA TTTTCTGGAT AGCTGTCGAG CTAGTACTCT GTTGGCAGAG
    TTAGATGATG ATGAGGATCT ACCTGAACCT GATGAAGAAG ATGATGAAAA TGAAGATGAT
    AACCAAGAAG ATCAAGAATA TGAGGAAGTT ATGGAAGAAG AGGAGTATGA GACCAAAGGA
    GGTCGTAGAA GAACATGGGA TGATGATTAT GTATTGAAAC GACAGTTTTC TGCTCTGGTT
    CCTGCTTTTG ACCCAAGACC TGGTCGAACA AATGTTCAAC AAACAACTGA TTTAGAAATC
    CCTCCACCGG GTACACCTCA TTCAGAACTC TTGGAAGAGG TTGAGTGCAT GCCTTCACCA
    CGCTTAGCAC TTACGTTGAA AGTTTCAGGC CTTGGAACAA CACGAGAAGT AGAACTGCCC
    CTAACAAATT TTCGATTCAC AATATTTTAC TACGTACAGA AATTGTTACA GCTTTCCTGT
    AATGGCAGTG TGAAATCTGA CAAACTTAGA CGTATTTGGG AGCCAACATA CACAATCATG
    TACAGAGAAA TGAAGGATTC TGATAAAGAA AAAGAAAGTG GAAAAATGGG TTGCTGGTCA
    GTAGAGCATG TGGAGCAGTA CCTTGGCACT GATGAATTAC CAAAGAATGA CTTGATAACC
    TACCTGCAGA AGAATGCAGA TTCAGCTTTC CTGCGCCACT GGAAATTAAC CGGCACTAAT
    AAAAGTATTA GGAAAAACAG AAATTGTTCC CAGCTCATAG CTGCATACAA GGATTTTTGT
    GAACATGGAT CAAAATCTGG ATTAAGCCAG GGGGCCATTT CTACTCTTCA GAATTCTGAT
    ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA GCCAAAGCTG GCAGTGGGCA GAACTCCTGT
    GGAGTAGAAG ATGTTTTGCA GTTACTTCGC ATTTTGTATA TAGTTGCTAG TGACCCTTAC
    ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT GAGCATCCTC AGTTTAACTT TCCACCAGAT
    GAATTCACAA GCAAAAAAAT TACTACAAAA ATTCTGCAAC AGATCGAGGA ACCCTTGGCA
    CTAGCAAGTG GAGCTTTGCC AGACTGGTGT GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA
    ATTCCATTTG AAACTAGACA GTTATACTTC ACCTGCACAG CGTTTGGAGC ATCAAGAGCA
    ATTGTGTGGC TACAAAACAG ACGCGAAGCC ACTGTTGAAA GAACAAGAAC CACAAGCACA
    GTGCGACGTG ATGATCCGGG AGAGTTCAGA GTGGGGCGCC TGAAGCATGA AAGGGTGAAA
    GTTCCGCGAG GTGAATCCCT GATGGAATGG GCAGAAAACG TCATGCAGAT TCATGCAGAC
    AGAAAATCTG TTCTAGAGGT TGAGTTTTTA GGGGAGGAAG GAACGGGCCT GGGTCCTACC
    TTGGAATTTT ATGCATTGGT AGCAGCAGAA TTTCAGAGGA CAGATCTGGG AGCTTGGCTT
    TGTGATGATG ATTTTCCAGA TGATGAGTCT CGTCAGGTTG ATATTGGTGG TGGATTAAAA
    CCCCCTGGTT ACTATGTACA GCGATCATGT GGACTCTTCA CAGCACCATT CCCACAAGAT
    AGTGATGAGC TTGAAAGGAT AACCAAACTC TTTCATTTCC TTGGAATTTT CTTGGCTAAA
    TGCATTCAAG ACAATAGACT TGTGGATTTA CCCATTTCTA AGCCTTTTTT TAAACTCATG
    TGTATGGGAG ACATTAAAAG CAATATGAGT AAGTTGATAT ATGAATCACG GAGTGACAGG
    GACTTGCACT GTACTGAAAG TCAGTCAGAG GCTTCTACAG AAGAAGGGCA CGATTCATTG
    TCAGTAGGAA GTTTGGAAGA AGACTCCAAA TCAGAATTTA TTCTTGATCC ACCAAAACCT
    AAACCTCCTG CTTGGTTTAA TGGAATTCTA ACGTGGGAAG ACTTTGAATT AATAAATCCA
    CATAGAGCAA GGTTTCTAAA AGAAATTAAG GACCTAGCTA TTAAAAGGCG TCAAATTTTA
    AGCAACAAAA GTCTGTCAGA AGATGAAAAA AATACAAAAC TACAGGAATT AATGCTAAAG
    AATCCATCAG GTTCTGGACC ACCACTTAGC ATAGAGGATT TGGGTTTAAA TTTTCAGTTT
    TGCCCTTCAT CCAAAGTGTA TGGATTTACA GCTGTAGATC TCAAACCAGG TGGTGAAGAT
    GAGACTGTAA CGATGGATAA TGCAGAGGAG TATGTGGATC TCATGTTTGA CTTCTGTATG
    CATACTGGTA TACAGAAACA GATGGAGGCA TTCAGAGATG GCTTCAATAG AGTCTTCCCA
    ATGGAGAAGC TGAGTTCTTT CAGCCATGAA GAAGTTCAGA TGATTCTTTG TGGAAATCAG
    TCACCTTCCT GGGCAGCAGA GGATATCATC AATTATACAG AACCTAAACT GGGATATACA
    CGGGACAGCC CTGGATTCCT TCGATTTGTG AGAGTTCTGT GTGGTATGTC TTCAGATGAA
    AGAAAGGCTT TCCTCCAGTT CACCACTGGT TGTTCAACAC TCCCACCAGG AGGACTGGCA
    AACCTACATC CCCGGCTGAC TGTTGTACGC AAGGTTGATG CTACAGATGC CAGTTACCCA
    TCTGTGAATA CATGTGTGCA TTACCTGAAG TTGCCTGAGT ATTCTTCTGA GGAGATTATG
    AGAGAGCGTC TGCTAGCTGC TACAATGGAG AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004941799.1
    CDS309..8039
    Translation

    Target ORF information:

    RefSeq Version XM_004941742.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004941742.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATACTTT TAATTATTTC
    CAGGATCTCC TAAGATCTGA ACTTCCAGAT TCTATAGAAA GTGCGTTGCA GGGTGATGAG
    AGATGTGTAC TGGATACCAT GCGGTTAGTA GATCTTCTTT TGGTGCTACT GTTCGAAGGG
    AGAAAAGCCC TGCCAAAATC CAGTGCTGGA TCTACAGGCA GAATTCCAGG ATTGCGGAGA
    CTGGATAGTT CTGGTGAACG TTCTCATCGG CAGCTGATAG ACTGCATCCG CAGTAAGGAT
    ACCGATGCAT TAATAGATGC TATTGACACG GGAGCTTTTG AAGTGAATTT TATGGATGAC
    GTAGGACAAA CTCTTTTAAA CTGGGCCTCA GCTTTTGGAA CTCAGGAAAT GGTAGAATTT
    CTCTGTGAAA GGGGTGCTGA TGTTAACAGA GGGCAGAGGT CATCCTCATT ACATTATGCA
    GCATGTTTTG GAAGACCTCA GGTAGCAAAG ACTCTCTTAC GGCATGGTGC AAACCCTGAT
    TTAAGAGATG AAGATGGGAA AACTCCTCTA GACAAAGCTC GTGAAAGAGG TCACAGTGAA
    GTAGTAGCTA TTCTACAGTC TCCAGGGGAT TGGATGTGTC CTGTTAACAA AGGAGATGAG
    AAGAAAAAGA AAGATGCAAA CAAGGATGAG GAAGAATGTA ATGAACCCAA AGGAGATCCA
    GAAATGGCAC CTATATACTT GAAAAGATTA TTGCCTGTAT TTGCACAAAC ATTTCAACAA
    ACTATGTTGC CTTCAATAAG GAAAGCAAGT CTTGCTCTCA TTAGAAAAAT GATACATTTT
    TGTTCTGAGG CACTGCTGAA GGAGGTTTGT GACTCTGATG CTGGCCACAA TCTGCCCACA
    ATACTGGTTG AGATAACAGC TACAGTTCTT GATCAAGAGG ATGATGATGA TGGCCATTTG
    CTAGCCTTGC AAATCATAAG GGATTTGGTG GATAAAGGTG GTGATCTTTT TCTAGACCAA
    CTGGCTAGAC TTGGTGTAAT CAGTAAAGTG TCAACTTTGG CAGGACCTTC ATCTGATGAT
    GAGAATGAAG AGGAGTCTAA ACCTGAGAAA GAGGATGAAC CACAGGAGGA TGCTAAAGAG
    CTGCAGCAGG GTAAACCATA TCACTGGAGA GACTGGTCAA TCATCAGGGG AAGGGACTGC
    CTGTACATAT GGAGTGATGC TGCAGCCCTG GAACTATCTA ATGGTAGTAA TGGATGGTTC
    AGGTTTATCT TGGATGGAAA ACTGGCCACA ATGTACTCCA GCGGAAGCCC AGAGGGAGGA
    TCTGATAGTT CAGAAAGTAG GAGTGAATTC CTTGAGAAGC TGCAAAGAGC TCGAAGCCAA
    GTAAAGCCAT CTACTGCAAG TCAGCCAATT TTATCAGCAC CCGGGCCAAC TAAACTTACT
    GTGGGAAATT GGTCACTTAC TTGTTTAAAA GAAGGAGAAA TTGCTATCCA CAATTCAGAT
    GGTCAGCAAG CTACAATACT GAAAGAAGAT CTGCCAGGCT TTGTGTTTGA ATCTAACAGA
    GGAACAAAGC ATTCGTTTAC AGCTGAAACC TCTCTGGGAT CAGAATTTGT GACTGGTTGG
    ACTGGCAAAA GAGGAAGGAA GCTAAAATCT AAACTGGAGA AGACAAAGCA GAAGGTACGC
    ACTATGGCAA GAGATTTATA CGATGATCAT TTTAAAGCTG TTGAAAGCAT GCCTCGAGGA
    GTAGTTGTAA CACTGAGGAA CATAGCAACA CAGTTAGAGT CTGCGTGGGA ACTTCATACA
    AACAGACAGT GTATTGAGGG AGAAAACACT TGGAGAGATT TAATGAAGAC TGCTTTGGAA
    AATTTAATTG TGCTTTTGAA GGATGAGAAT ACCATTTCTC CATACGAAAT GTGCAGTAGT
    GGATTAGTTC AATCATTGCT TACAGTTTTA AATAACAATG TTGATTTAGA TATGAAACAA
    GACTGTAGTC AACTAGTTGA AAGAATAAAT GTATTCAAAA CAGCATTCAG CGAAAATGAA
    GATGATGAAA GTCGACCAGC AGTTGCATTA ATTCGGAAAT TAATAGCAGT GTTAGAATCT
    ATTGAACGTC TACCTCTTCA CTTGTATGAT ACACCAGGAT CTACTTATAA TCTTCAGATA
    TTGACAAGAC GTTTGAGGTT TCGTTTGGAA CGTGCTTCAG GGGAGACATC CTTGATAGAT
    CGAACTGGCA GAATGCTAAA GATGGAGCCA TTGGCAACTG TTGAATCTCT AGAACAGTAT
    CTTCTGAAAA TGGTTGCAAA GCAATGGTAC GACTTTGATA GGGCTTCATT TGTTTTTGTA
    CGAAAACTGC GTGAAGGCCA AACATTTGTA TTCAGGCATC AACATGATTT TGATGAAAAT
    GGAATTATTT ACTGGATTGG GACAAATGCG AAAACTGCAT ATGAGTGGGT AAATCCAGCA
    GCCTATGGGT TAGTAGTGGT TACATCCTCA GAAGGAAGAA ATCTGCCTTA TGGCCGTTTA
    GAAGACATCC TAAGCCGTGA CAGTTCAGCT CTCAATTGTC ATACTAATGA TGATAAGAAT
    GCTTGGTTTG CCATAGATCT TGGCCTTTGG GTTATACCAT CAGCTTACAC GTTACGCCAT
    GCTCGAGGTT ATGGACGATC TGCTCTCAGA AATTGGGTTT TCCAGGTATC TAAGGATGGA
    CAGAACTGGA CTACTTTGTA TACTCATGTT GATGATTGTA GTCTCAACGA ACCAGGGTCT
    ACAGCAACAT GGCCTTTAGA TCCTCCAAAG GATGAGAAAC AAGGTTGGAG GCATGTTAGA
    ATAAAACAGA TGGGAAAGAA TGCCAGTGGG CAAACACACT ACCTCTCTCT GTCTGGATTT
    GAACTTTATG GCACTGTGAA TGGAGTATGT GAAGACCAGT TGGGAAAAGC TGCTAAAGAA
    GCTGAAGCAA ATCTGCGAAG GCAGCGACGT CTGGTCCGGT CTCAAGTTTT GAAATACATG
    GTTCCAGGAG CTCGGGTAAT CAGAGGAATT GATTGGAAGT GGAGAGATCA GGATGGAAGC
    CCACAGGGTG AAGGCACTGT TACAGGAGAA TTGCACAATG GCTGGATTGA TGTCACCTGG
    GATGCTGGTG GCTCTAACTC TTACCGTATG GGCGCAGAAG GAAAGTTTGA CCTCAAGCTT
    GCACCAGGGT ACGACCCTGA TTCAGCGGCA TCACCCAAAC CTGTTTCATC CACTGTTTCA
    GGCACAACGC AGTCATGGAG CAGCTTGGTG AAAAACAACT GTCCAGACAA GACGACTGCT
    GCTGCAGGCT CCTCAAGTAG AAAAGGAAGC AGCAGTTCTG TGTGTAGCGT GGCCAGTAGC
    AGCGACATCA GCTTGGGTTC GACCAAAATG GAGCGGAGAT CAGAAAGTGT AATGGAGCAA
    AATATAGTTT CAGGCACTGA CGTCCACGAA CCAATTGTTG TTCTGTCTTC CGCTGATAAT
    GTACCTCAGG CTGAAGTAGG GTCATCTTCC AGTGCAAGCA CCAGCACCTT AACAGCGGAC
    ATGGGAAATG AAAATACAGA AAGGAAGTTA GGTCCTGATA ATTCGATACG TACTCCTGGG
    GAGTCCAGTG CTATCTCCAT GGGAATTGTT AGTGTAAGTT CTCCTGATGT TAGTTCAGTT
    TCCGAACTGA CTAATAAAGA AGCAGCATCC CAACGACCTC TGAGCTCTTC AGCAAGTAAC
    AGACTTTCAG TTAGCTCTTT GTTGGCTGCT GGGGCGCCAA TGAGCTCCAG TGCAAGCGTT
    CCTAACTTAT CATCTCGAGA AACTTCGAGC CTGGAGTCTT TTGTACGAAG AGTGGCAAAC
    ATAGCACGTA CCAATGCCAC AAACAACATG AATTTAAGCC GAAGTAGCAG TGATAACAAT
    ACTAATACTC TGGGAAGAAA CGTCATGAGT ACTGCAACGT CTCCTCTTAT GGGTGCCCAA
    AGTTTTCCTA ATCTGACTAC AACTGGTACC ACATCAACAG TGACCATGTC TACATCCAGT
    GTTACTAGCA GCAGCAATGT AGCTACAGCA ACTACAGTTT TATCTGTTGG TCAGTCGCTT
    AGTAATACTC TAACTACTAG CCTAACATCA ACATCTAGTG AGAGCGATAC AGGGCAGGAG
    GCAGAATATT CTTTATATGA TTTTCTGGAT AGCTGTCGAG CTAGTACTCT GTTGGCAGAG
    TTAGATGATG ATGAGGATCT ACCTGAACCT GATGAAGAAG ATGATGAAAA TGAAGATGAT
    AACCAAGAAG ATCAAGAATA TGAGGAAGTT ATGGAAGAAG AGGAGTATGA GACCAAAGGA
    GGTCGTAGAA GAACATGGGA TGATGATTAT GTATTGAAAC GACAGTTTTC TGCTCTGGTT
    CCTGCTTTTG ACCCAAGACC TGGTCGAACA AATGTTCAAC AAACAACTGA TTTAGAAATC
    CCTCCACCGG GTACACCTCA TTCAGAACTC TTGGAAGAGG TTGAGTGCAT GCCTTCACCA
    CGCTTAGCAC TTACGTTGAA AGTTTCAGGC CTTGGAACAA CACGAGAAGT AGAACTGCCC
    CTAACAAATT TTCGATTCAC AATATTTTAC TACGTACAGA AATTGTTACA GCTTTCCTGT
    AATGGCAGTG TGAAATCTGA CAAACTTAGA CGTATTTGGG AGCCAACATA CACAATCATG
    TACAGAGAAA TGAAGGATTC TGATAAAGAA AAAGAAAGTG GAAAAATGGG TTGCTGGTCA
    GTAGAGCATG TGGAGCAGTA CCTTGGCACT GATGAATTAC CAAAGAATGA CTTGATAACC
    TACCTGCAGA AGAATGCAGA TTCAGCTTTC CTGCGCCACT GGAAATTAAC CGGCACTAAT
    AAAAGTATTA GGAAAAACAG AAATTGTTCC CAGCTCATAG CTGCATACAA GGATTTTTGT
    GAACATGGAT CAAAATCTGG ATTAAGCCAG GGGGCCATTT CTACTCTTCA GAATTCTGAT
    ATTCTTAGTT TGGCAAAAGA GCAGCCTCAA GCCAAAGCTG GCAGTGGGCA GAACTCCTGT
    GGAGTAGAAG ATGTTTTGCA GTTACTTCGC ATTTTGTATA TAGTTGCTAG TGACCCTTAC
    ACAGCGCGAA CTTCTCAAGA AGAAGGAGAT GAGCATCCTC AGTTTAACTT TCCACCAGAT
    GAATTCACAA GCAAAAAAAT TACTACAAAA ATTCTGCAAC AGATCGAGGA ACCCTTGGCA
    CTAGCAAGTG GAGCTTTGCC AGACTGGTGT GAGCAGCTAA CAAGCAAGTG TCCTTTCTTA
    ATTCCATTTG AAACTAGACA GTTATACTTC ACCTGCACAG CGTTTGGAGC ATCAAGAGCA
    ATTGTGTGGC TACAAAACAG ACGCGAAGCC ACTGTTGAAA GAACAAGAAC CACAAGCACA
    GTGCGACGTG ATGATCCGGG AGAGTTCAGA GTGGGGCGCC TGAAGCATGA AAGGGTGAAA
    GTTCCGCGAG GTGAATCCCT GATGGAATGG GCAGAAAACG TCATGCAGAT TCATGCAGAC
    AGAAAATCTG TTCTAGAGGT TGAGTTTTTA GGGGAGGAAG GAACGGGCCT GGGTCCTACC
    TTGGAATTTT ATGCATTGGT AGCAGCAGAA TTTCAGAGGA CAGATCTGGG AGCTTGGCTT
    TGTGATGATG ATTTTCCAGA TGATGAGTCT CGTCAGGTTG ATATTGGTGG TGGATTAAAA
    CCCCCTGGTT ACTATGTACA GCGATCATGT GGACTCTTCA CAGCACCATT CCCACAAGAT
    AGTGATGAGC TTGAAAGGAT AACCAAACTC TTTCATTTCC TTGGAATTTT CTTGGCTAAA
    TGCATTCAAG ACAATAGACT TGTGGATTTA CCCATTTCTA AGCCTTTTTT TAAACTCATG
    TGTATGGGAG ACATTAAAAG CAATATGAGT AAGTTGATAT ATGAATCACG GAGTGACAGG
    GACTTGCACT GTACTGAAAG TCAGTCAGAG GCTTCTACAG AAGAAGGGCA CGATTCATTG
    TCAGTAGGAA GTTTGGAAGA AGACTCCAAA TCAGAATTTA TTCTTGATCC ACCAAAACCT
    AAACCTCCTG CTTGGTTTAA TGGAATTCTA ACGTGGGAAG ACTTTGAATT AATAAATCCA
    CATAGAGCAA GGTTTCTAAA AGAAATTAAG GACCTAGCTA TTAAAAGGCG TCAAATTTTA
    AGCAACAAAA GTCTGTCAGA AGATGAAAAA AATACAAAAC TACAGGAATT AATGCTAAAG
    AATCCATCAG GTTCTGGACC ACCACTTAGC ATAGAGGATT TGGGTTTAAA TTTTCAGTTT
    TGCCCTTCAT CCAAAGTGTA TGGATTTACA GCTGTAGATC TCAAACCAGG TGGTGAAGAT
    GAGACTGTAA CGATGGATAA TGCAGAGGAG TATGTGGATC TCATGTTTGA CTTCTGTATG
    CATACTGGTA TACAGAAACA GATGGAGGCA TTCAGAGATG GCTTCAATAG AGTCTTCCCA
    ATGGAGAAGC TGAGTTCTTT CAGCCATGAA GAAGTTCAGA TGATTCTTTG TGGAAATCAG
    TCACCTTCCT GGGCAGCAGA GGATATCATC AATTATACAG AACCTAAACT GGGATATACA
    CGGGACAGCC CTGGATTCCT TCGATTTGTG AGAGTTCTGT GTGGTATGTC TTCAGATGAA
    AGAAAGGCTT TCCTCCAGTT CACCACTGGT TGTTCAACAC TCCCACCAGG AGGACTGGCA
    AACCTACATC CCCGGCTGAC TGTTGTACGC AAGGTTGATG CTACAGATGC CAGTTACCCA
    TCTGTGAATA CATGTGTGCA TTACCTGAAG TTGCCTGAGT ATTCTTCTGA GGAGATTATG
    AGAGAGCGTC TGCTAGCTGC TACAATGGAG AAAGGCTTCC ATCTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09385
    Clone ID Related Accession (Same CDS sequence) XM_421227.5 , XM_421227.6
    Accession Version XM_421227.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_421227.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG ATCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGAAGG
    AAGCTAAAAT CTAAACTGGA GAAGACAAAG CAGAAGGTAC GCACTATGGC AAGAGATTTA
    TACGATGATC ATTTTAAAGC TGTTGAAAGC ATGCCTCGAG GAGTAGTTGT AACACTGAGG
    AACATAGCAA CACAGTTAGA GTCTGCGTGG GAACTTCATA CAAACAGACA GTGTATTGAG
    GGAGAAAACA CTTGGAGAGA TTTAATGAAG ACTGCTTTGG AAAATTTAAT TGTGCTTTTG
    AAGGATGAGA ATACCATTTC TCCATACGAA ATGTGCAGTA GTGGATTAGT TCAATCATTG
    CTTACAGTTT TAAATAACAA TGTTGATTTA GATATGAAAC AAGACTGTAG TCAACTAGTT
    GAAAGAATAA ATGTATTCAA AACAGCATTC AGCGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCA GTGTTAGAAT CTATTGAACG TCTACCTCTT
    CACTTGTATG ATACACCAGG ATCTACTTAT AATCTTCAGA TATTGACAAG ACGTTTGAGG
    TTTCGTTTGG AACGTGCTTC AGGGGAGACA TCCTTGATAG ATCGAACTGG CAGAATGCTA
    AAGATGGAGC CATTGGCAAC TGTTGAATCT CTAGAACAGT ATCTTCTGAA AATGGTTGCA
    AAGCAATGGT ACGACTTTGA TAGGGCTTCA TTTGTTTTTG TACGAAAACT GCGTGAAGGC
    CAAACATTTG TATTCAGGCA TCAACATGAT TTTGATGAAA ATGGAATTAT TTACTGGATT
    GGGACAAATG CGAAAACTGC ATATGAGTGG GTAAATCCAG CAGCCTATGG GTTAGTAGTG
    GTTACATCCT CAGAAGGAAG AAATCTGCCT TATGGCCGTT TAGAAGACAT CCTAAGCCGT
    GACAGTTCAG CTCTCAATTG TCATACTAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTTGGCCTTT GGGTTATACC ATCAGCTTAC ACGTTACGCC ATGCTCGAGG TTATGGACGA
    TCTGCTCTCA GAAATTGGGT TTTCCAGGTA TCTAAGGATG GACAGAACTG GACTACTTTG
    TATACTCATG TTGATGATTG TAGTCTCAAC GAACCAGGGT CTACAGCAAC ATGGCCTTTA
    GATCCTCCAA AGGATGAGAA ACAAGGTTGG AGGCATGTTA GAATAAAACA GATGGGAAAG
    AATGCCAGTG GGCAAACACA CTACCTCTCT CTGTCTGGAT TTGAACTTTA TGGCACTGTG
    AATGGAGTAT GTGAAGACCA GTTGGGAAAA GCTGCTAAAG AAGCTGAAGC AAATCTGCGA
    AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT TTGAAATACA TGGTTCCAGG AGCTCGGGTA
    ATCAGAGGAA TTGATTGGAA GTGGAGAGAT CAGGATGGAA GCCCACAGGG TGAAGGCACT
    GTTACAGGAG AATTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCTAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAGTTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATTCAGCGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGACTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA TGGAGCGGAG ATCAGAAAGT GTAATGGAGC AAAATATAGT TTCAGGCACT
    GACGTCCACG AACCAATTGT TGTTCTGTCT TCCGCTGATA ATGTACCTCA GGCTGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG ACATGGGAAA TGAAAATACA
    GAAAGGAAGT TAGGTCCTGA TAATTCGATA CGTACTCCTG GGGAGTCCAG TGCTATCTCC
    ATGGGAATTG TTAGTGTAAG TTCTCCTGAT GTTAGTTCAG TTTCCGAACT GACTAATAAA
    GAAGCAGCAT CCCAACGACC TCTGAGCTCT TCAGCAAGTA ACAGACTTTC AGTTAGCTCT
    TTGTTGGCTG CTGGGGCGCC AATGAGCTCC AGTGCAAGCG TTCCTAACTT ATCATCTCGA
    GAAACTTCGA GCCTGGAGTC TTTTGTACGA AGAGTGGCAA ACATAGCACG TACCAATGCC
    ACAAACAACA TGAATTTAAG CCGAAGTAGC AGTGATAACA ATACTAATAC TCTGGGAAGA
    AACGTCATGA GTACTGCAAC GTCTCCTCTT ATGGGTGCCC AAAGTTTTCC TAATCTGACT
    ACAACTGGTA CCACATCAAC AGTGACCATG TCTACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACTACAGT TTTATCTGTT GGTCAGTCGC TTAGTAATAC TCTAACTACT
    AGCCTAACAT CAACATCTAG TGAGAGCGAT ACAGGGCAGG AGGCAGAATA TTCTTTATAT
    GATTTTCTGG ATAGCTGTCG AGCTAGTACT CTGTTGGCAG AGTTAGATGA TGATGAGGAT
    CTACCTGAAC CTGATGAAGA AGATGATGAA AATGAAGATG ATAACCAAGA AGATCAAGAA
    TATGAGGAAG TTATGGAAGA AGAGGAGTAT GAGACCAAAG GAGGTCGTAG AAGAACATGG
    GATGATGATT ATGTATTGAA ACGACAGTTT TCTGCTCTGG TTCCTGCTTT TGACCCAAGA
    CCTGGTCGAA CAAATGTTCA ACAAACAACT GATTTAGAAA TCCCTCCACC GGGTACACCT
    CATTCAGAAC TCTTGGAAGA GGTTGAGTGC ATGCCTTCAC CACGCTTAGC ACTTACGTTG
    AAAGTTTCAG GCCTTGGAAC AACACGAGAA GTAGAACTGC CCCTAACAAA TTTTCGATTC
    ACAATATTTT ACTACGTACA GAAATTGTTA CAGCTTTCCT GTAATGGCAG TGTGAAATCT
    GACAAACTTA GACGTATTTG GGAGCCAACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAAGAAAG TGGAAAAATG GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATTCAGCTT TCCTGCGCCA CTGGAAATTA ACCGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CCCAGCTCAT AGCTGCATAC AAGGATTTTT GTGAACATGG ATCAAAATCT
    GGATTAAGCC AGGGGGCCAT TTCTACTCTT CAGAATTCTG ATATTCTTAG TTTGGCAAAA
    GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG CAGAACTCCT GTGGAGTAGA AGATGTTTTG
    CAGTTACTTC GCATTTTGTA TATAGTTGCT AGTGACCCTT ACACAGCGCG AACTTCTCAA
    GAAGAAGGAG ATGAGCATCC TCAGTTTAAC TTTCCACCAG ATGAATTCAC AAGCAAAAAA
    ATTACTACAA AAATTCTGCA ACAGATCGAG GAACCCTTGG CACTAGCAAG TGGAGCTTTG
    CCAGACTGGT GTGAGCAGCT AACAAGCAAG TGTCCTTTCT TAATTCCATT TGAAACTAGA
    CAGTTATACT TCACCTGCAC AGCGTTTGGA GCATCAAGAG CAATTGTGTG GCTACAAAAC
    AGACGCGAAG CCACTGTTGA AAGAACAAGA ACCACAAGCA CAGTGCGACG TGATGATCCG
    GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT GAAAGGGTGA AAGTTCCGCG AGGTGAATCC
    CTGATGGAAT GGGCAGAAAA CGTCATGCAG ATTCATGCAG ACAGAAAATC TGTTCTAGAG
    GTTGAGTTTT TAGGGGAGGA AGGAACGGGC CTGGGTCCTA CCTTGGAATT TTATGCATTG
    GTAGCAGCAG AATTTCAGAG GACAGATCTG GGAGCTTGGC TTTGTGATGA TGATTTTCCA
    GATGATGAGT CTCGTCAGGT TGATATTGGT GGTGGATTAA AACCCCCTGG TTACTATGTA
    CAGCGATCAT GTGGACTCTT CACAGCACCA TTCCCACAAG ATAGTGATGA GCTTGAAAGG
    ATAACCAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCTA AATGCATTCA AGACAATAGA
    CTTGTGGATT TACCCATTTC TAAGCCTTTT TTTAAACTCA TGTGTATGGG AGACATTAAA
    AGCAATATGA GTAAGTTGAT ATATGAATCA CGGAGTGACA GGGACTTGCA CTGTACTGAA
    AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG CACGATTCAT TGTCAGTAGG AAGTTTGGAA
    GAAGACTCCA AATCAGAATT TATTCTTGAT CCACCAAAAC CTAAACCTCC TGCTTGGTTT
    AATGGAATTC TAACGTGGGA AGACTTTGAA TTAATAAATC CACATAGAGC AAGGTTTCTA
    AAAGAAATTA AGGACCTAGC TATTAAAAGG CGTCAAATTT TAAGCAACAA AAGTCTGTCA
    GAAGATGAAA AAAATACAAA ACTACAGGAA TTAATGCTAA AGAATCCATC AGGTTCTGGA
    CCACCACTTA GCATAGAGGA TTTGGGTTTA AATTTTCAGT TTTGCCCTTC ATCCAAAGTG
    TATGGATTTA CAGCTGTAGA TCTCAAACCA GGTGGTGAAG ATGAGACTGT AACGATGGAT
    AATGCAGAGG AGTATGTGGA TCTCATGTTT GACTTCTGTA TGCATACTGG TATACAGAAA
    CAGATGGAGG CATTCAGAGA TGGCTTCAAT AGAGTCTTCC CAATGGAGAA GCTGAGTTCT
    TTCAGCCATG AAGAAGTTCA GATGATTCTT TGTGGAAATC AGTCACCTTC CTGGGCAGCA
    GAGGATATCA TCAATTATAC AGAACCTAAA CTGGGATATA CACGGGACAG CCCTGGATTC
    CTTCGATTTG TGAGAGTTCT GTGTGGTATG TCTTCAGATG AAAGAAAGGC TTTCCTCCAG
    TTCACCACTG GTTGTTCAAC ACTCCCACCA GGAGGACTGG CAAACCTACA TCCCCGGCTG
    ACTGTTGTAC GCAAGGTTGA TGCTACAGAT GCCAGTTACC CATCTGTGAA TACATGTGTG
    CATTACCTGA AGTTGCCTGA GTATTCTTCT GAGGAGATTA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT CCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_421227.3
    CDS286..7998
    Translation

    Target ORF information:

    RefSeq Version XM_421227.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_421227.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG ATCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGAAGG
    AAGCTAAAAT CTAAACTGGA GAAGACAAAG CAGAAGGTAC GCACTATGGC AAGAGATTTA
    TACGATGATC ATTTTAAAGC TGTTGAAAGC ATGCCTCGAG GAGTAGTTGT AACACTGAGG
    AACATAGCAA CACAGTTAGA GTCTGCGTGG GAACTTCATA CAAACAGACA GTGTATTGAG
    GGAGAAAACA CTTGGAGAGA TTTAATGAAG ACTGCTTTGG AAAATTTAAT TGTGCTTTTG
    AAGGATGAGA ATACCATTTC TCCATACGAA ATGTGCAGTA GTGGATTAGT TCAATCATTG
    CTTACAGTTT TAAATAACAA TGTTGATTTA GATATGAAAC AAGACTGTAG TCAACTAGTT
    GAAAGAATAA ATGTATTCAA AACAGCATTC AGCGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCA GTGTTAGAAT CTATTGAACG TCTACCTCTT
    CACTTGTATG ATACACCAGG ATCTACTTAT AATCTTCAGA TATTGACAAG ACGTTTGAGG
    TTTCGTTTGG AACGTGCTTC AGGGGAGACA TCCTTGATAG ATCGAACTGG CAGAATGCTA
    AAGATGGAGC CATTGGCAAC TGTTGAATCT CTAGAACAGT ATCTTCTGAA AATGGTTGCA
    AAGCAATGGT ACGACTTTGA TAGGGCTTCA TTTGTTTTTG TACGAAAACT GCGTGAAGGC
    CAAACATTTG TATTCAGGCA TCAACATGAT TTTGATGAAA ATGGAATTAT TTACTGGATT
    GGGACAAATG CGAAAACTGC ATATGAGTGG GTAAATCCAG CAGCCTATGG GTTAGTAGTG
    GTTACATCCT CAGAAGGAAG AAATCTGCCT TATGGCCGTT TAGAAGACAT CCTAAGCCGT
    GACAGTTCAG CTCTCAATTG TCATACTAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTTGGCCTTT GGGTTATACC ATCAGCTTAC ACGTTACGCC ATGCTCGAGG TTATGGACGA
    TCTGCTCTCA GAAATTGGGT TTTCCAGGTA TCTAAGGATG GACAGAACTG GACTACTTTG
    TATACTCATG TTGATGATTG TAGTCTCAAC GAACCAGGGT CTACAGCAAC ATGGCCTTTA
    GATCCTCCAA AGGATGAGAA ACAAGGTTGG AGGCATGTTA GAATAAAACA GATGGGAAAG
    AATGCCAGTG GGCAAACACA CTACCTCTCT CTGTCTGGAT TTGAACTTTA TGGCACTGTG
    AATGGAGTAT GTGAAGACCA GTTGGGAAAA GCTGCTAAAG AAGCTGAAGC AAATCTGCGA
    AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT TTGAAATACA TGGTTCCAGG AGCTCGGGTA
    ATCAGAGGAA TTGATTGGAA GTGGAGAGAT CAGGATGGAA GCCCACAGGG TGAAGGCACT
    GTTACAGGAG AATTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCTAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAGTTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATTCAGCGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGACTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA TGGAGCGGAG ATCAGAAAGT GTAATGGAGC AAAATATAGT TTCAGGCACT
    GACGTCCACG AACCAATTGT TGTTCTGTCT TCCGCTGATA ATGTACCTCA GGCTGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG ACATGGGAAA TGAAAATACA
    GAAAGGAAGT TAGGTCCTGA TAATTCGATA CGTACTCCTG GGGAGTCCAG TGCTATCTCC
    ATGGGAATTG TTAGTGTAAG TTCTCCTGAT GTTAGTTCAG TTTCCGAACT GACTAATAAA
    GAAGCAGCAT CCCAACGACC TCTGAGCTCT TCAGCAAGTA ACAGACTTTC AGTTAGCTCT
    TTGTTGGCTG CTGGGGCGCC AATGAGCTCC AGTGCAAGCG TTCCTAACTT ATCATCTCGA
    GAAACTTCGA GCCTGGAGTC TTTTGTACGA AGAGTGGCAA ACATAGCACG TACCAATGCC
    ACAAACAACA TGAATTTAAG CCGAAGTAGC AGTGATAACA ATACTAATAC TCTGGGAAGA
    AACGTCATGA GTACTGCAAC GTCTCCTCTT ATGGGTGCCC AAAGTTTTCC TAATCTGACT
    ACAACTGGTA CCACATCAAC AGTGACCATG TCTACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACTACAGT TTTATCTGTT GGTCAGTCGC TTAGTAATAC TCTAACTACT
    AGCCTAACAT CAACATCTAG TGAGAGCGAT ACAGGGCAGG AGGCAGAATA TTCTTTATAT
    GATTTTCTGG ATAGCTGTCG AGCTAGTACT CTGTTGGCAG AGTTAGATGA TGATGAGGAT
    CTACCTGAAC CTGATGAAGA AGATGATGAA AATGAAGATG ATAACCAAGA AGATCAAGAA
    TATGAGGAAG TTATGGAAGA AGAGGAGTAT GAGACCAAAG GAGGTCGTAG AAGAACATGG
    GATGATGATT ATGTATTGAA ACGACAGTTT TCTGCTCTGG TTCCTGCTTT TGACCCAAGA
    CCTGGTCGAA CAAATGTTCA ACAAACAACT GATTTAGAAA TCCCTCCACC GGGTACACCT
    CATTCAGAAC TCTTGGAAGA GGTTGAGTGC ATGCCTTCAC CACGCTTAGC ACTTACGTTG
    AAAGTTTCAG GCCTTGGAAC AACACGAGAA GTAGAACTGC CCCTAACAAA TTTTCGATTC
    ACAATATTTT ACTACGTACA GAAATTGTTA CAGCTTTCCT GTAATGGCAG TGTGAAATCT
    GACAAACTTA GACGTATTTG GGAGCCAACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAAGAAAG TGGAAAAATG GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATTCAGCTT TCCTGCGCCA CTGGAAATTA ACCGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CCCAGCTCAT AGCTGCATAC AAGGATTTTT GTGAACATGG ATCAAAATCT
    GGATTAAGCC AGGGGGCCAT TTCTACTCTT CAGAATTCTG ATATTCTTAG TTTGGCAAAA
    GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG CAGAACTCCT GTGGAGTAGA AGATGTTTTG
    CAGTTACTTC GCATTTTGTA TATAGTTGCT AGTGACCCTT ACACAGCGCG AACTTCTCAA
    GAAGAAGGAG ATGAGCATCC TCAGTTTAAC TTTCCACCAG ATGAATTCAC AAGCAAAAAA
    ATTACTACAA AAATTCTGCA ACAGATCGAG GAACCCTTGG CACTAGCAAG TGGAGCTTTG
    CCAGACTGGT GTGAGCAGCT AACAAGCAAG TGTCCTTTCT TAATTCCATT TGAAACTAGA
    CAGTTATACT TCACCTGCAC AGCGTTTGGA GCATCAAGAG CAATTGTGTG GCTACAAAAC
    AGACGCGAAG CCACTGTTGA AAGAACAAGA ACCACAAGCA CAGTGCGACG TGATGATCCG
    GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT GAAAGGGTGA AAGTTCCGCG AGGTGAATCC
    CTGATGGAAT GGGCAGAAAA CGTCATGCAG ATTCATGCAG ACAGAAAATC TGTTCTAGAG
    GTTGAGTTTT TAGGGGAGGA AGGAACGGGC CTGGGTCCTA CCTTGGAATT TTATGCATTG
    GTAGCAGCAG AATTTCAGAG GACAGATCTG GGAGCTTGGC TTTGTGATGA TGATTTTCCA
    GATGATGAGT CTCGTCAGGT TGATATTGGT GGTGGATTAA AACCCCCTGG TTACTATGTA
    CAGCGATCAT GTGGACTCTT CACAGCACCA TTCCCACAAG ATAGTGATGA GCTTGAAAGG
    ATAACCAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCTA AATGCATTCA AGACAATAGA
    CTTGTGGATT TACCCATTTC TAAGCCTTTT TTTAAACTCA TGTGTATGGG AGACATTAAA
    AGCAATATGA GTAAGTTGAT ATATGAATCA CGGAGTGACA GGGACTTGCA CTGTACTGAA
    AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG CACGATTCAT TGTCAGTAGG AAGTTTGGAA
    GAAGACTCCA AATCAGAATT TATTCTTGAT CCACCAAAAC CTAAACCTCC TGCTTGGTTT
    AATGGAATTC TAACGTGGGA AGACTTTGAA TTAATAAATC CACATAGAGC AAGGTTTCTA
    AAAGAAATTA AGGACCTAGC TATTAAAAGG CGTCAAATTT TAAGCAACAA AAGTCTGTCA
    GAAGATGAAA AAAATACAAA ACTACAGGAA TTAATGCTAA AGAATCCATC AGGTTCTGGA
    CCACCACTTA GCATAGAGGA TTTGGGTTTA AATTTTCAGT TTTGCCCTTC ATCCAAAGTG
    TATGGATTTA CAGCTGTAGA TCTCAAACCA GGTGGTGAAG ATGAGACTGT AACGATGGAT
    AATGCAGAGG AGTATGTGGA TCTCATGTTT GACTTCTGTA TGCATACTGG TATACAGAAA
    CAGATGGAGG CATTCAGAGA TGGCTTCAAT AGAGTCTTCC CAATGGAGAA GCTGAGTTCT
    TTCAGCCATG AAGAAGTTCA GATGATTCTT TGTGGAAATC AGTCACCTTC CTGGGCAGCA
    GAGGATATCA TCAATTATAC AGAACCTAAA CTGGGATATA CACGGGACAG CCCTGGATTC
    CTTCGATTTG TGAGAGTTCT GTGTGGTATG TCTTCAGATG AAAGAAAGGC TTTCCTCCAG
    TTCACCACTG GTTGTTCAAC ACTCCCACCA GGAGGACTGG CAAACCTACA TCCCCGGCTG
    ACTGTTGTAC GCAAGGTTGA TGCTACAGAT GCCAGTTACC CATCTGTGAA TACATGTGTG
    CATTACCTGA AGTTGCCTGA GTATTCTTCT GAGGAGATTA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT CCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa09385
    Clone ID Related Accession (Same CDS sequence) XM_421227.5 , XM_421227.6
    Accession Version XM_421227.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_421227.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG ATCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGAAGG
    AAGCTAAAAT CTAAACTGGA GAAGACAAAG CAGAAGGTAC GCACTATGGC AAGAGATTTA
    TACGATGATC ATTTTAAAGC TGTTGAAAGC ATGCCTCGAG GAGTAGTTGT AACACTGAGG
    AACATAGCAA CACAGTTAGA GTCTGCGTGG GAACTTCATA CAAACAGACA GTGTATTGAG
    GGAGAAAACA CTTGGAGAGA TTTAATGAAG ACTGCTTTGG AAAATTTAAT TGTGCTTTTG
    AAGGATGAGA ATACCATTTC TCCATACGAA ATGTGCAGTA GTGGATTAGT TCAATCATTG
    CTTACAGTTT TAAATAACAA TGTTGATTTA GATATGAAAC AAGACTGTAG TCAACTAGTT
    GAAAGAATAA ATGTATTCAA AACAGCATTC AGCGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCA GTGTTAGAAT CTATTGAACG TCTACCTCTT
    CACTTGTATG ATACACCAGG ATCTACTTAT AATCTTCAGA TATTGACAAG ACGTTTGAGG
    TTTCGTTTGG AACGTGCTTC AGGGGAGACA TCCTTGATAG ATCGAACTGG CAGAATGCTA
    AAGATGGAGC CATTGGCAAC TGTTGAATCT CTAGAACAGT ATCTTCTGAA AATGGTTGCA
    AAGCAATGGT ACGACTTTGA TAGGGCTTCA TTTGTTTTTG TACGAAAACT GCGTGAAGGC
    CAAACATTTG TATTCAGGCA TCAACATGAT TTTGATGAAA ATGGAATTAT TTACTGGATT
    GGGACAAATG CGAAAACTGC ATATGAGTGG GTAAATCCAG CAGCCTATGG GTTAGTAGTG
    GTTACATCCT CAGAAGGAAG AAATCTGCCT TATGGCCGTT TAGAAGACAT CCTAAGCCGT
    GACAGTTCAG CTCTCAATTG TCATACTAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTTGGCCTTT GGGTTATACC ATCAGCTTAC ACGTTACGCC ATGCTCGAGG TTATGGACGA
    TCTGCTCTCA GAAATTGGGT TTTCCAGGTA TCTAAGGATG GACAGAACTG GACTACTTTG
    TATACTCATG TTGATGATTG TAGTCTCAAC GAACCAGGGT CTACAGCAAC ATGGCCTTTA
    GATCCTCCAA AGGATGAGAA ACAAGGTTGG AGGCATGTTA GAATAAAACA GATGGGAAAG
    AATGCCAGTG GGCAAACACA CTACCTCTCT CTGTCTGGAT TTGAACTTTA TGGCACTGTG
    AATGGAGTAT GTGAAGACCA GTTGGGAAAA GCTGCTAAAG AAGCTGAAGC AAATCTGCGA
    AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT TTGAAATACA TGGTTCCAGG AGCTCGGGTA
    ATCAGAGGAA TTGATTGGAA GTGGAGAGAT CAGGATGGAA GCCCACAGGG TGAAGGCACT
    GTTACAGGAG AATTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCTAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAGTTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATTCAGCGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGACTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA TGGAGCGGAG ATCAGAAAGT GTAATGGAGC AAAATATAGT TTCAGGCACT
    GACGTCCACG AACCAATTGT TGTTCTGTCT TCCGCTGATA ATGTACCTCA GGCTGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG ACATGGGAAA TGAAAATACA
    GAAAGGAAGT TAGGTCCTGA TAATTCGATA CGTACTCCTG GGGAGTCCAG TGCTATCTCC
    ATGGGAATTG TTAGTGTAAG TTCTCCTGAT GTTAGTTCAG TTTCCGAACT GACTAATAAA
    GAAGCAGCAT CCCAACGACC TCTGAGCTCT TCAGCAAGTA ACAGACTTTC AGTTAGCTCT
    TTGTTGGCTG CTGGGGCGCC AATGAGCTCC AGTGCAAGCG TTCCTAACTT ATCATCTCGA
    GAAACTTCGA GCCTGGAGTC TTTTGTACGA AGAGTGGCAA ACATAGCACG TACCAATGCC
    ACAAACAACA TGAATTTAAG CCGAAGTAGC AGTGATAACA ATACTAATAC TCTGGGAAGA
    AACGTCATGA GTACTGCAAC GTCTCCTCTT ATGGGTGCCC AAAGTTTTCC TAATCTGACT
    ACAACTGGTA CCACATCAAC AGTGACCATG TCTACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACTACAGT TTTATCTGTT GGTCAGTCGC TTAGTAATAC TCTAACTACT
    AGCCTAACAT CAACATCTAG TGAGAGCGAT ACAGGGCAGG AGGCAGAATA TTCTTTATAT
    GATTTTCTGG ATAGCTGTCG AGCTAGTACT CTGTTGGCAG AGTTAGATGA TGATGAGGAT
    CTACCTGAAC CTGATGAAGA AGATGATGAA AATGAAGATG ATAACCAAGA AGATCAAGAA
    TATGAGGAAG TTATGGAAGA AGAGGAGTAT GAGACCAAAG GAGGTCGTAG AAGAACATGG
    GATGATGATT ATGTATTGAA ACGACAGTTT TCTGCTCTGG TTCCTGCTTT TGACCCAAGA
    CCTGGTCGAA CAAATGTTCA ACAAACAACT GATTTAGAAA TCCCTCCACC GGGTACACCT
    CATTCAGAAC TCTTGGAAGA GGTTGAGTGC ATGCCTTCAC CACGCTTAGC ACTTACGTTG
    AAAGTTTCAG GCCTTGGAAC AACACGAGAA GTAGAACTGC CCCTAACAAA TTTTCGATTC
    ACAATATTTT ACTACGTACA GAAATTGTTA CAGCTTTCCT GTAATGGCAG TGTGAAATCT
    GACAAACTTA GACGTATTTG GGAGCCAACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAAGAAAG TGGAAAAATG GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATTCAGCTT TCCTGCGCCA CTGGAAATTA ACCGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CCCAGCTCAT AGCTGCATAC AAGGATTTTT GTGAACATGG ATCAAAATCT
    GGATTAAGCC AGGGGGCCAT TTCTACTCTT CAGAATTCTG ATATTCTTAG TTTGGCAAAA
    GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG CAGAACTCCT GTGGAGTAGA AGATGTTTTG
    CAGTTACTTC GCATTTTGTA TATAGTTGCT AGTGACCCTT ACACAGCGCG AACTTCTCAA
    GAAGAAGGAG ATGAGCATCC TCAGTTTAAC TTTCCACCAG ATGAATTCAC AAGCAAAAAA
    ATTACTACAA AAATTCTGCA ACAGATCGAG GAACCCTTGG CACTAGCAAG TGGAGCTTTG
    CCAGACTGGT GTGAGCAGCT AACAAGCAAG TGTCCTTTCT TAATTCCATT TGAAACTAGA
    CAGTTATACT TCACCTGCAC AGCGTTTGGA GCATCAAGAG CAATTGTGTG GCTACAAAAC
    AGACGCGAAG CCACTGTTGA AAGAACAAGA ACCACAAGCA CAGTGCGACG TGATGATCCG
    GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT GAAAGGGTGA AAGTTCCGCG AGGTGAATCC
    CTGATGGAAT GGGCAGAAAA CGTCATGCAG ATTCATGCAG ACAGAAAATC TGTTCTAGAG
    GTTGAGTTTT TAGGGGAGGA AGGAACGGGC CTGGGTCCTA CCTTGGAATT TTATGCATTG
    GTAGCAGCAG AATTTCAGAG GACAGATCTG GGAGCTTGGC TTTGTGATGA TGATTTTCCA
    GATGATGAGT CTCGTCAGGT TGATATTGGT GGTGGATTAA AACCCCCTGG TTACTATGTA
    CAGCGATCAT GTGGACTCTT CACAGCACCA TTCCCACAAG ATAGTGATGA GCTTGAAAGG
    ATAACCAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCTA AATGCATTCA AGACAATAGA
    CTTGTGGATT TACCCATTTC TAAGCCTTTT TTTAAACTCA TGTGTATGGG AGACATTAAA
    AGCAATATGA GTAAGTTGAT ATATGAATCA CGGAGTGACA GGGACTTGCA CTGTACTGAA
    AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG CACGATTCAT TGTCAGTAGG AAGTTTGGAA
    GAAGACTCCA AATCAGAATT TATTCTTGAT CCACCAAAAC CTAAACCTCC TGCTTGGTTT
    AATGGAATTC TAACGTGGGA AGACTTTGAA TTAATAAATC CACATAGAGC AAGGTTTCTA
    AAAGAAATTA AGGACCTAGC TATTAAAAGG CGTCAAATTT TAAGCAACAA AAGTCTGTCA
    GAAGATGAAA AAAATACAAA ACTACAGGAA TTAATGCTAA AGAATCCATC AGGTTCTGGA
    CCACCACTTA GCATAGAGGA TTTGGGTTTA AATTTTCAGT TTTGCCCTTC ATCCAAAGTG
    TATGGATTTA CAGCTGTAGA TCTCAAACCA GGTGGTGAAG ATGAGACTGT AACGATGGAT
    AATGCAGAGG AGTATGTGGA TCTCATGTTT GACTTCTGTA TGCATACTGG TATACAGAAA
    CAGATGGAGG CATTCAGAGA TGGCTTCAAT AGAGTCTTCC CAATGGAGAA GCTGAGTTCT
    TTCAGCCATG AAGAAGTTCA GATGATTCTT TGTGGAAATC AGTCACCTTC CTGGGCAGCA
    GAGGATATCA TCAATTATAC AGAACCTAAA CTGGGATATA CACGGGACAG CCCTGGATTC
    CTTCGATTTG TGAGAGTTCT GTGTGGTATG TCTTCAGATG AAAGAAAGGC TTTCCTCCAG
    TTCACCACTG GTTGTTCAAC ACTCCCACCA GGAGGACTGG CAAACCTACA TCCCCGGCTG
    ACTGTTGTAC GCAAGGTTGA TGCTACAGAT GCCAGTTACC CATCTGTGAA TACATGTGTG
    CATTACCTGA AGTTGCCTGA GTATTCTTCT GAGGAGATTA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT CCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_421227.3
    CDS309..8021
    Translation

    Target ORF information:

    RefSeq Version XM_421227.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_421227.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA CACATTGCTG GAATGGCTGC AGATGGGGCA GGGAGATGAA 
    AGGGATATGC AGCTGATAGC ACTGGAACAG CTATGCATGC TACTTCTGAT GTCAGACAAC
    GTGGATCGCT GTTTTGAAAC ATGTCCTCCT CGAACTTTTT TACCAGCACT GTGCAAAATT
    TTTCTTGATG AAAGTGCACC AGACAATGTA TTAGAAGTAA CAGCTCGTGC CATCACATAT
    TATCTGGACG TATCAGCAGA ATGTACCCGT AGGATTGTTG GAGTTGATGG AGCTATCAAG
    GCACTTTGTA ATCGTTTAGT AGTTGTTGAG CTTAACAACA GAACCAGCAG AGATTTGGCT
    GAGCAGTGCG TGAAGGTGTT AGAATTAATC TGTACCCGTG AGTCAGGAGC TGTGTTTGAA
    GCTGGAGGGT TGAATTGTGT CCTGACATTT ATTCGTGACA GTGGACACCT TGTTCATAAA
    GATACTTTAC ATTCTGCTAT GGCTGTAGTA TCCAGGCTGT GTGGCAAAAT GGAACCTCAG
    GATTCTTCAT TAGAGATCTG TGTGGAATCA CTGTCTAGTT TATTAAAACA TGAGGACCAT
    CAGGTTTCGG ATGGAGCTCT GCGATGTTTT GCTTCATTAG CTGACAGATT CACACGTCGT
    GGAGTTGACC CAGCTCCATT AGCTAAGCAT GGATTAACTG AGGAGTTGTT ATCTCGCATG
    GCAGCTGCTG GTGGCACAGC ATCAGGACCA TCTTCAGCTT GCAAACCTGG CCGAACTAGC
    ACTGGAGCAC CATCTACAGC TGCAGACTCT AAATTAAGTA ATCAGGTGTC AACTATTGTA
    AGCCTGTTGT CAACCCTGTG TAGAGGCTCT CCAGTAGTAA CACATGATCT CCTAAGATCT
    GAACTTCCAG ATTCTATAGA AAGTGCGTTG CAGGGTGATG AGAGATGTGT ACTGGATACC
    ATGCGGTTAG TAGATCTTCT TTTGGTGCTA CTGTTCGAAG GGAGAAAAGC CCTGCCAAAA
    TCCAGTGCTG GATCTACAGG CAGAATTCCA GGATTGCGGA GACTGGATAG TTCTGGTGAA
    CGTTCTCATC GGCAGCTGAT AGACTGCATC CGCAGTAAGG ATACCGATGC ATTAATAGAT
    GCTATTGACA CGGGAGCTTT TGAAGTGAAT TTTATGGATG ACGTAGGACA AACTCTTTTA
    AACTGGGCCT CAGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTCTGTGA AAGGGGTGCT
    GATGTTAACA GAGGGCAGAG GTCATCCTCA TTACATTATG CAGCATGTTT TGGAAGACCT
    CAGGTAGCAA AGACTCTCTT ACGGCATGGT GCAAACCCTG ATTTAAGAGA TGAAGATGGG
    AAAACTCCTC TAGACAAAGC TCGTGAAAGA GGTCACAGTG AAGTAGTAGC TATTCTACAG
    TCTCCAGGGG ATTGGATGTG TCCTGTTAAC AAAGGAGATG AGAAGAAAAA GAAAGATGCA
    AACAAGGATG AGGAAGAATG TAATGAACCC AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGAT TATTGCCTGT ATTTGCACAA ACATTTCAAC AAACTATGTT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT CATTAGAAAA ATGATACATT TTTGTTCTGA GGCACTGCTG
    AAGGAGGTTT GTGACTCTGA TGCTGGCCAC AATCTGCCCA CAATACTGGT TGAGATAACA
    GCTACAGTTC TTGATCAAGA GGATGATGAT GATGGCCATT TGCTAGCCTT GCAAATCATA
    AGGGATTTGG TGGATAAAGG TGGTGATCTT TTTCTAGACC AACTGGCTAG ACTTGGTGTA
    ATCAGTAAAG TGTCAACTTT GGCAGGACCT TCATCTGATG ATGAGAATGA AGAGGAGTCT
    AAACCTGAGA AAGAGGATGA ACCACAGGAG GATGCTAAAG AGCTGCAGCA GGGTAAACCA
    TATCACTGGA GAGACTGGTC AATCATCAGG GGAAGGGACT GCCTGTACAT ATGGAGTGAT
    GCTGCAGCCC TGGAACTATC TAATGGTAGT AATGGATGGT TCAGGTTTAT CTTGGATGGA
    AAACTGGCCA CAATGTACTC CAGCGGAAGC CCAGAGGGAG GATCTGATAG TTCAGAAAGT
    AGGAGTGAAT TCCTTGAGAA GCTGCAAAGA GCTCGAAGCC AAGTAAAGCC ATCTACTGCA
    AGTCAGCCAA TTTTATCAGC ACCCGGGCCA ACTAAACTTA CTGTGGGAAA TTGGTCACTT
    ACTTGTTTAA AAGAAGGAGA AATTGCTATC CACAATTCAG ATGGTCAGCA AGCTACAATA
    CTGAAAGAAG ATCTGCCAGG CTTTGTGTTT GAATCTAACA GAGGAACAAA GCATTCGTTT
    ACAGCTGAAA CCTCTCTGGG ATCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGAAGG
    AAGCTAAAAT CTAAACTGGA GAAGACAAAG CAGAAGGTAC GCACTATGGC AAGAGATTTA
    TACGATGATC ATTTTAAAGC TGTTGAAAGC ATGCCTCGAG GAGTAGTTGT AACACTGAGG
    AACATAGCAA CACAGTTAGA GTCTGCGTGG GAACTTCATA CAAACAGACA GTGTATTGAG
    GGAGAAAACA CTTGGAGAGA TTTAATGAAG ACTGCTTTGG AAAATTTAAT TGTGCTTTTG
    AAGGATGAGA ATACCATTTC TCCATACGAA ATGTGCAGTA GTGGATTAGT TCAATCATTG
    CTTACAGTTT TAAATAACAA TGTTGATTTA GATATGAAAC AAGACTGTAG TCAACTAGTT
    GAAAGAATAA ATGTATTCAA AACAGCATTC AGCGAAAATG AAGATGATGA AAGTCGACCA
    GCAGTTGCAT TAATTCGGAA ATTAATAGCA GTGTTAGAAT CTATTGAACG TCTACCTCTT
    CACTTGTATG ATACACCAGG ATCTACTTAT AATCTTCAGA TATTGACAAG ACGTTTGAGG
    TTTCGTTTGG AACGTGCTTC AGGGGAGACA TCCTTGATAG ATCGAACTGG CAGAATGCTA
    AAGATGGAGC CATTGGCAAC TGTTGAATCT CTAGAACAGT ATCTTCTGAA AATGGTTGCA
    AAGCAATGGT ACGACTTTGA TAGGGCTTCA TTTGTTTTTG TACGAAAACT GCGTGAAGGC
    CAAACATTTG TATTCAGGCA TCAACATGAT TTTGATGAAA ATGGAATTAT TTACTGGATT
    GGGACAAATG CGAAAACTGC ATATGAGTGG GTAAATCCAG CAGCCTATGG GTTAGTAGTG
    GTTACATCCT CAGAAGGAAG AAATCTGCCT TATGGCCGTT TAGAAGACAT CCTAAGCCGT
    GACAGTTCAG CTCTCAATTG TCATACTAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTTGGCCTTT GGGTTATACC ATCAGCTTAC ACGTTACGCC ATGCTCGAGG TTATGGACGA
    TCTGCTCTCA GAAATTGGGT TTTCCAGGTA TCTAAGGATG GACAGAACTG GACTACTTTG
    TATACTCATG TTGATGATTG TAGTCTCAAC GAACCAGGGT CTACAGCAAC ATGGCCTTTA
    GATCCTCCAA AGGATGAGAA ACAAGGTTGG AGGCATGTTA GAATAAAACA GATGGGAAAG
    AATGCCAGTG GGCAAACACA CTACCTCTCT CTGTCTGGAT TTGAACTTTA TGGCACTGTG
    AATGGAGTAT GTGAAGACCA GTTGGGAAAA GCTGCTAAAG AAGCTGAAGC AAATCTGCGA
    AGGCAGCGAC GTCTGGTCCG GTCTCAAGTT TTGAAATACA TGGTTCCAGG AGCTCGGGTA
    ATCAGAGGAA TTGATTGGAA GTGGAGAGAT CAGGATGGAA GCCCACAGGG TGAAGGCACT
    GTTACAGGAG AATTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCTAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAGTTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATTCAGCGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGACTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA TGGAGCGGAG ATCAGAAAGT GTAATGGAGC AAAATATAGT TTCAGGCACT
    GACGTCCACG AACCAATTGT TGTTCTGTCT TCCGCTGATA ATGTACCTCA GGCTGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG ACATGGGAAA TGAAAATACA
    GAAAGGAAGT TAGGTCCTGA TAATTCGATA CGTACTCCTG GGGAGTCCAG TGCTATCTCC
    ATGGGAATTG TTAGTGTAAG TTCTCCTGAT GTTAGTTCAG TTTCCGAACT GACTAATAAA
    GAAGCAGCAT CCCAACGACC TCTGAGCTCT TCAGCAAGTA ACAGACTTTC AGTTAGCTCT
    TTGTTGGCTG CTGGGGCGCC AATGAGCTCC AGTGCAAGCG TTCCTAACTT ATCATCTCGA
    GAAACTTCGA GCCTGGAGTC TTTTGTACGA AGAGTGGCAA ACATAGCACG TACCAATGCC
    ACAAACAACA TGAATTTAAG CCGAAGTAGC AGTGATAACA ATACTAATAC TCTGGGAAGA
    AACGTCATGA GTACTGCAAC GTCTCCTCTT ATGGGTGCCC AAAGTTTTCC TAATCTGACT
    ACAACTGGTA CCACATCAAC AGTGACCATG TCTACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACTACAGT TTTATCTGTT GGTCAGTCGC TTAGTAATAC TCTAACTACT
    AGCCTAACAT CAACATCTAG TGAGAGCGAT ACAGGGCAGG AGGCAGAATA TTCTTTATAT
    GATTTTCTGG ATAGCTGTCG AGCTAGTACT CTGTTGGCAG AGTTAGATGA TGATGAGGAT
    CTACCTGAAC CTGATGAAGA AGATGATGAA AATGAAGATG ATAACCAAGA AGATCAAGAA
    TATGAGGAAG TTATGGAAGA AGAGGAGTAT GAGACCAAAG GAGGTCGTAG AAGAACATGG
    GATGATGATT ATGTATTGAA ACGACAGTTT TCTGCTCTGG TTCCTGCTTT TGACCCAAGA
    CCTGGTCGAA CAAATGTTCA ACAAACAACT GATTTAGAAA TCCCTCCACC GGGTACACCT
    CATTCAGAAC TCTTGGAAGA GGTTGAGTGC ATGCCTTCAC CACGCTTAGC ACTTACGTTG
    AAAGTTTCAG GCCTTGGAAC AACACGAGAA GTAGAACTGC CCCTAACAAA TTTTCGATTC
    ACAATATTTT ACTACGTACA GAAATTGTTA CAGCTTTCCT GTAATGGCAG TGTGAAATCT
    GACAAACTTA GACGTATTTG GGAGCCAACA TACACAATCA TGTACAGAGA AATGAAGGAT
    TCTGATAAAG AAAAAGAAAG TGGAAAAATG GGTTGCTGGT CAGTAGAGCA TGTGGAGCAG
    TACCTTGGCA CTGATGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATTCAGCTT TCCTGCGCCA CTGGAAATTA ACCGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CCCAGCTCAT AGCTGCATAC AAGGATTTTT GTGAACATGG ATCAAAATCT
    GGATTAAGCC AGGGGGCCAT TTCTACTCTT CAGAATTCTG ATATTCTTAG TTTGGCAAAA
    GAGCAGCCTC AAGCCAAAGC TGGCAGTGGG CAGAACTCCT GTGGAGTAGA AGATGTTTTG
    CAGTTACTTC GCATTTTGTA TATAGTTGCT AGTGACCCTT ACACAGCGCG AACTTCTCAA
    GAAGAAGGAG ATGAGCATCC TCAGTTTAAC TTTCCACCAG ATGAATTCAC AAGCAAAAAA
    ATTACTACAA AAATTCTGCA ACAGATCGAG GAACCCTTGG CACTAGCAAG TGGAGCTTTG
    CCAGACTGGT GTGAGCAGCT AACAAGCAAG TGTCCTTTCT TAATTCCATT TGAAACTAGA
    CAGTTATACT TCACCTGCAC AGCGTTTGGA GCATCAAGAG CAATTGTGTG GCTACAAAAC
    AGACGCGAAG CCACTGTTGA AAGAACAAGA ACCACAAGCA CAGTGCGACG TGATGATCCG
    GGAGAGTTCA GAGTGGGGCG CCTGAAGCAT GAAAGGGTGA AAGTTCCGCG AGGTGAATCC
    CTGATGGAAT GGGCAGAAAA CGTCATGCAG ATTCATGCAG ACAGAAAATC TGTTCTAGAG
    GTTGAGTTTT TAGGGGAGGA AGGAACGGGC CTGGGTCCTA CCTTGGAATT TTATGCATTG
    GTAGCAGCAG AATTTCAGAG GACAGATCTG GGAGCTTGGC TTTGTGATGA TGATTTTCCA
    GATGATGAGT CTCGTCAGGT TGATATTGGT GGTGGATTAA AACCCCCTGG TTACTATGTA
    CAGCGATCAT GTGGACTCTT CACAGCACCA TTCCCACAAG ATAGTGATGA GCTTGAAAGG
    ATAACCAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCTA AATGCATTCA AGACAATAGA
    CTTGTGGATT TACCCATTTC TAAGCCTTTT TTTAAACTCA TGTGTATGGG AGACATTAAA
    AGCAATATGA GTAAGTTGAT ATATGAATCA CGGAGTGACA GGGACTTGCA CTGTACTGAA
    AGTCAGTCAG AGGCTTCTAC AGAAGAAGGG CACGATTCAT TGTCAGTAGG AAGTTTGGAA
    GAAGACTCCA AATCAGAATT TATTCTTGAT CCACCAAAAC CTAAACCTCC TGCTTGGTTT
    AATGGAATTC TAACGTGGGA AGACTTTGAA TTAATAAATC CACATAGAGC AAGGTTTCTA
    AAAGAAATTA AGGACCTAGC TATTAAAAGG CGTCAAATTT TAAGCAACAA AAGTCTGTCA
    GAAGATGAAA AAAATACAAA ACTACAGGAA TTAATGCTAA AGAATCCATC AGGTTCTGGA
    CCACCACTTA GCATAGAGGA TTTGGGTTTA AATTTTCAGT TTTGCCCTTC ATCCAAAGTG
    TATGGATTTA CAGCTGTAGA TCTCAAACCA GGTGGTGAAG ATGAGACTGT AACGATGGAT
    AATGCAGAGG AGTATGTGGA TCTCATGTTT GACTTCTGTA TGCATACTGG TATACAGAAA
    CAGATGGAGG CATTCAGAGA TGGCTTCAAT AGAGTCTTCC CAATGGAGAA GCTGAGTTCT
    TTCAGCCATG AAGAAGTTCA GATGATTCTT TGTGGAAATC AGTCACCTTC CTGGGCAGCA
    GAGGATATCA TCAATTATAC AGAACCTAAA CTGGGATATA CACGGGACAG CCCTGGATTC
    CTTCGATTTG TGAGAGTTCT GTGTGGTATG TCTTCAGATG AAAGAAAGGC TTTCCTCCAG
    TTCACCACTG GTTGTTCAAC ACTCCCACCA GGAGGACTGG CAAACCTACA TCCCCGGCTG
    ACTGTTGTAC GCAAGGTTGA TGCTACAGAT GCCAGTTACC CATCTGTGAA TACATGTGTG
    CATTACCTGA AGTTGCCTGA GTATTCTTCT GAGGAGATTA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT CCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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