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TMEM53 cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol TMEM53
Entrez Gene ID 424581
Full Name transmembrane protein 53
General protein information
Preferred Names

transmembrane protein 53

Names

transmembrane protein 53
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015291002.1 XP_015146488.1 transmembrane protein 53 isoform X1
NM_001277580.1 NP_001264509.1 transmembrane protein 53
XM_015291002.2 XP_015146488.1 transmembrane protein 53 isoform X1
XM_015291003.2 XP_015146489.1 transmembrane protein 53 isoform X1
XM_015291003.1 XP_015146489.1 transmembrane protein 53 isoform X1

Pathways from BioSystems


Homologies


TMEM53 XP_002801564.1
Bos taurus (cattle) TMEM53 NP_001033661.1
Mus musculus (house mouse) Tmem53 NP_001272741.1
Rattus norvegicus (Norway rat) Tmem53 NP_001101434.1
Danio rerio (zebrafish) tmem53 NP_001002637.1
Oryza sativa (rice) Os06g0730300 NP_001058655.1
Xenopus tropicalis (tropical clawed frog) tmem53 NP_001107322.1
Caenorhabditis elegans (roundworm) C09B8.4 NP_509006.2
Pan troglodytes (chimpanzee) TMEM53 XP_513099.3
Gallus gallus (chicken) TMEM53 NP_001264509.1
Homo sapiens (human) TMEM53 NP_078863.2
Caenorhabditis elegans (roundworm) T10B11.6 NP_491980.1
Caenorhabditis elegans (roundworm) T24H10.4 NP_495945.2
Arabidopsis thaliana (thale cress) AT3G19970 NP_566652.1
Canis lupus familiaris (dog) TMEM53 XP_005629146.1
Drosophila melanogaster (fruit fly) CG8245 NP_610178.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009586 XP_318608.3

Gene Ontology


Bibliography

Related articles in PubMed

ESTs from brain and testis of White Leghorn and red junglefowl: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels.
Savolainen P, Fitzsimmons C, Arvestad L, Andersson L, Lundeberg J
Cytogenetic and genome research111(1)79-87(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TMEM53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa25569 NM_001277580.1
Latest version!		 Gallus gallus transmembrane protein 53 (TMEM53), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
OGa39359 XM_015291002.2
Latest version!		 Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00
OGa39359 XM_015291003.2
Latest version!		 Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00
View Old Accession Versions >>
OGa39359 XM_015291002.1 Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00
OGa39359 XM_015291003.1 Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa25569
Clone ID Related Accession (Same CDS sequence) NM_001277580.1
Accession Version NM_001277580.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 831bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-20
Organism Gallus gallus(chicken)
Product transmembrane protein 53
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN233827.1, BX934725.1 and BX933889.2. On Apr 12, 2013 this sequence version replaced XM_422420.3. Sequence Note: This RefSeq record was created from transcript data from different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CN233827.1, BX934725.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCCG GCGAGCTGGA GGCAACGGTG GAGCTGGCGC CGGGGGCGGC CCGCGGGAGC 
AGCACGGAGA AAGGGCACGC AGGTGGGCAG CCCGTGGTGA TCCTTCTGGG CTGGGCAGGC 
TGCCAGGACA GGCATCTGGC CAAGTACAGC GCGTTGTACA GTCAGAAGGG GTGCACCGTC 
ATCCGCTACA CAGCTCCATG GAGGATGATA TTCTTCTCTG AGAGCTTTGG CATCAAATCC 
CTCCAGACCC CAGCCAAGAG ACTCCTGGAG CTGCTCTTTG ACTACAGCAT TGAGAACAGG 
CCGGTGCTTT TCCATGTCTT TAGCAATGGT GGTGTCATGC TCTACCGTTA CATCACTGAA 
GCACTTCGCA CTCAGCAGCC CTTTAAGAAC CTCAGAGTCG CAGGCACTGT TTTTGATAGT 
GCCCCTGGCA GAAGAAACTT GAGAGGGGCC CTTCGTGCCT TGGCAACTGT CCTAGCATCC 
ACAAACGTGC TGCTCAGGTA TCTCCTTATG CTCACTTTTG CTACTACAGT TGTCCTGCTG 
CGGATCCTGC TGTACCCACT GACTCGCTTC ATCCACGAGA GCCACTACGA TGCCCTGCTG 
AAGGCACCCA CCCGGTGGCC TGAGCTCTAC CTCTACTCCC AGGCAGATGC CATCATCAGT 
GCCAGTGATA TTAAGCACAT GGCTGACGCA CGGCAGCAGC TTGGTGTCTC CGTGAAAGCT 
GTAGACTTCA CAGATTCAGC TCACGTCAGC CATCTGCGGG TGTATCCCAC CTACTACAGC 
ACCCTCTGTA CGACTTTCCT GTCTGACTGT GTCAGGGGCT CGCCTTGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001264509.1
CDS28..858
Translation

Target ORF information:

RefSeq Version NM_001277580.1
Organism Gallus gallus(chicken)
Definition Gallus gallus transmembrane protein 53 (TMEM53), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001277580.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGGCCG GCGAGCTGGA GGCAACGGTG GAGCTGGCGC CGGGGGCGGC CCGCGGGAGC 
AGCACGGAGA AAGGGCACGC AGGTGGGCAG CCCGTGGTGA TCCTTCTGGG CTGGGCAGGC 
TGCCAGGACA GGCATCTGGC CAAGTACAGC GCGTTGTACA GTCAGAAGGG GTGCACCGTC 
ATCCGCTACA CAGCTCCATG GAGGATGATA TTCTTCTCTG AGAGCTTTGG CATCAAATCC 
CTCCAGACCC CAGCCAAGAG ACTCCTGGAG CTGCTCTTTG ACTACAGCAT TGAGAACAGG 
CCGGTGCTTT TCCATGTCTT TAGCAATGGT GGTGTCATGC TCTACCGTTA CATCACTGAA 
GCACTTCGCA CTCAGCAGCC CTTTAAGAAC CTCAGAGTCG CAGGCACTGT TTTTGATAGT 
GCCCCTGGCA GAAGAAACTT GAGAGGGGCC CTTCGTGCCT TGGCAACTGT CCTAGCATCC 
ACAAACGTGC TGCTCAGGTA TCTCCTTATG CTCACTTTTG CTACTACAGT TGTCCTGCTG 
CGGATCCTGC TGTACCCACT GACTCGCTTC ATCCACGAGA GCCACTACGA TGCCCTGCTG 
AAGGCACCCA CCCGGTGGCC TGAGCTCTAC CTCTACTCCC AGGCAGATGC CATCATCAGT 
GCCAGTGATA TTAAGCACAT GGCTGACGCA CGGCAGCAGC TTGGTGTCTC CGTGAAAGCT 
GTAGACTTCA CAGATTCAGC TCACGTCAGC CATCTGCGGG TGTATCCCAC CTACTACAGC 
ACCCTCTGTA CGACTTTCCT GTCTGACTGT GTCAGGGGCT CGCCTTGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa39359
Clone ID Related Accession (Same CDS sequence) XM_015291002.1 , XM_015291002.2 , XM_015291003.2 , XM_015291003.1
Accession Version XM_015291002.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product transmembrane protein 53 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015146488.1
CDS257..883
Translation

Target ORF information:

RefSeq Version XM_015291002.1
Organism Gallus gallus(chicken)
Definition Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015291002.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa39359
Clone ID Related Accession (Same CDS sequence) XM_015291002.1 , XM_015291002.2 , XM_015291003.2 , XM_015291003.1
Accession Version XM_015291002.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product transmembrane protein 53 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015146488.1
CDS234..860
Translation

Target ORF information:

RefSeq Version XM_015291002.2
Organism Gallus gallus(chicken)
Definition Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015291002.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa39359
Clone ID Related Accession (Same CDS sequence) XM_015291002.1 , XM_015291002.2 , XM_015291003.2 , XM_015291003.1
Accession Version XM_015291003.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product transmembrane protein 53 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015146489.1
CDS171..797
Translation

Target ORF information:

RefSeq Version XM_015291003.2
Organism Gallus gallus(chicken)
Definition Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015291003.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa39359
Clone ID Related Accession (Same CDS sequence) XM_015291002.1 , XM_015291002.2 , XM_015291003.2 , XM_015291003.1
Accession Version XM_015291003.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product transmembrane protein 53 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015146489.1
CDS143..769
Translation

Target ORF information:

RefSeq Version XM_015291003.1
Organism Gallus gallus(chicken)
Definition Gallus gallus transmembrane protein 53 (TMEM53), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015291003.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATATTCT TCTCTGAGAG CTTTGGCATC AAATCCCTCC AGACCCCAGC CAAGAGACTC 
CTGGAGCTGC TCTTTGACTA CAGCATTGAG AACAGGCCGG TGCTTTTCCA TGTCTTTAGC 
AATGGTGGTG TCATGCTCTA CCGTTACATC ATTGAAGCAC TTCGCACTCA GCAGCCCTTT 
AAGAACCTCA GAGTCGCAGG CACTGTTTTT GATAGTGCCC CTGGCAGAAG AAACTTGAGA 
GGGGCCCTTC GTGCCTTGGC AACTGTCCTA GCATCCACAA ACGTGCTGCT CAGGTATCTC 
CTTATGCTCA CTTTTGCTAC TACAGTTGTC CTGCTGCGGA TCCTGCTGTA CCCACTGACT 
CGCTTCATCC ACGAGAGCCA CTACGATGCC CTGCTGAAGG CACCCACCCG GTGGCCTGAG 
CTTTACCTCT ACTCCCAGGC AGATGCCATC ATCAGTGCCA GTGATATTAA GCACATGGCT 
GACGCACGGC AGCAGCTTGG TGTCTCCGTG AAAGCTGTAG ACTTCACAGA TTCAGCTCAC 
GTCAGCCATC TGCGGGTGTA TCCCACCTAC TACAGCACCC TCTGTACGAC TTTCCTGTCT 
GACTGTGTCA GGGGCTCGCC TCGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

ESTs from brain and testis of White Leghorn and red junglefowl: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels.
Cytogenetic and genome research111(1)79-87(2005)
Savolainen P,Fitzsimmons C,Arvestad L,Andersson L,Lundeberg J