EIF4G1 cDNA ORF clone, Gallus gallus(chicken)

  • Gene

  • Clones

  • gRNAs

The following EIF4G1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4G1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152826 XM_025153667.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa39996 XM_015291541.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39996 XM_015291542.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa152824 XM_025153661.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa152825 XM_025153662.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa39989 XM_015291528.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_015291535.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_015291536.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_025153664.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_015291534.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39990 XM_015291529.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39991 XM_015291530.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39992 XM_015291531.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291532.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291539.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291538.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291537.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39994 XM_015291533.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39994 XM_025153666.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39994 XM_025153665.1
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39995 XM_015291540.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39997 XM_015291543.2
Latest version!
Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OGa39989 XM_015291528.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291532.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39992 XM_015291531.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_015291536.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291539.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39991 XM_015291530.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39997 XM_015291543.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39994 XM_015291533.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_015291535.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291538.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39989 XM_015291534.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39996 XM_015291541.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa39990 XM_015291529.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39993 XM_015291537.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39995 XM_015291540.1 Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152826
    Clone ID Related Accession (Same CDS sequence) XM_025153667.1
    Accession Version XM_025153667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGTATCC AGCTGCTTCC CAGGGTCGTT CCACATACGT TGTCCCAACA CAGCAGTACC 
    CGGTCCAGCC CGGTGCCCCT AGTTTTTACC CTGGAGCCAG CCCCACAGAG TTCGGGACTT
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009435.1
    CDS274..4737
    Translation

    Target ORF information:

    RefSeq Version XM_025153667.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGTATCC AGCTGCTTCC CAGGGTCGTT CCACATACGT TGTCCCAACA CAGCAGTACC 
    CGGTCCAGCC CGGTGCCCCT AGTTTTTACC CTGGAGCCAG CCCCACAGAG TTCGGGACTT
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39996
    Clone ID Related Accession (Same CDS sequence) XM_015291541.2 , XM_015291542.1 , XM_015291541.1
    Accession Version XM_015291541.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA 
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147027.1
    CDS284..4573
    Translation

    Target ORF information:

    RefSeq Version XM_015291541.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291541.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA 
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39996
    Clone ID Related Accession (Same CDS sequence) XM_015291541.2 , XM_015291542.1 , XM_015291541.1
    Accession Version XM_015291542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA 
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147028.1
    CDS75..4364
    Translation

    Target ORF information:

    RefSeq Version XM_015291542.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA 
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39996
    Clone ID Related Accession (Same CDS sequence) XM_015291541.2 , XM_015291542.1 , XM_015291541.1
    Accession Version XM_015291541.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA 
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147027.1
    CDS244..4533
    Translation

    Target ORF information:

    RefSeq Version XM_015291541.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA 
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152824
    Clone ID Related Accession (Same CDS sequence) XM_025153661.1
    Accession Version XM_025153661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGGTCGT TCCACATACG TTGTCCCAAC ACAGCAGTAC
    CCGGTCCAGC CCGGTGCCCC TAGTTTTTAC CCTGGAGCCA GCCCCACAGA GTTCGGGACT
    TACGCGGGTG CCTATTACCC GGCCCAGGGG GTGCAGCAGT TCCCGGCGGG GGTCCCCACT
    GCCCAGGTCA TTGTGAGCCA GCAGCCACCG ATCCCCCCAA AACGAGAGCG CAAGACGATC
    CGGATACGAG ACCCCAACCA AGGTGGCAAA GACATCACTG AAGAAATTAT GTCCGGAGCA
    AGGACCTCAT CTACCCCCAC CCCTCCCCAG GCTGGAAGCG GTTTGGAACC CCAGGCCAAT
    GGAGAAACCC CTCATGTAGC AGTTATTGTT CGGCCAGATG ACCGCCCAAA GCCCGCGCTG
    GTGGTGAGCA AGCCCGTCTC CCTGGAGCCC AGCAAGTCAG CGTCCCCGTC GCCTCCCCCT
    CCCCTCATCC CCGAGGTGGA GCCTGTGGTG ATGTCAACTA TGACGCTGGT GCCAATGGAG
    CCCCCCATGG AAGCGGACAC TAAAGTGGAG CTGGGCGAGG CGCCGCCCGA CCTGCACCAG
    ACGTTTAGCG CTATCACTAC AGTGCCAGGG GCTGGGGAGC TGCCCCTTGT ACCCCCACCT
    GACATGGACA CAGCGGCTGT GGTGGAGGAG GAGGTGGCAG AGGAGGTGGA AGAGGTTGCC
    ATTCCTCTCC TGGAGCCCAC CTTGCAGGCG CCCGCCCTGC CCGAGGTGCC ATCGGTGCCT
    GCCGCCCCCC TCATGCCAGC CGTGCCCCCG GTGCCAGCTG CGCCGTCGCC GCCGCTCGTT
    GTCCCGCCGG TCCCTGAAGC ACCCGCCAAA CCCGCCTCTC CCAGCCCCCC GCCGCCCCAG
    GAAGAGCCCT GCCCTGAGCC TGTTGCTCAG CCTGCTGCTG AGGCCAATGG GGTCTTAGAG
    GAGGTGCCCC AGCCGCTCCC TGAGGCGCCC GTGTGCCAGC CGGTGCCTGT GCCCATATCG
    GAGCCTGCCC CGGTGCCCGC CCCAGCGCCC ACTCTGGACT CTCCCATTGC GCAGCCTGAG
    GAGCTGCCCT TGCCCAACGG GATGGAGGGC TCCAGCAAAG TGGAGGCGAG CGAGGAGCAG
    CCTGAGTCGG ACATCAGCCC CATCTCGGAG CCCGAGGAGC CAGTGCAGCC TGGAACCCCC
    GCCTCCCCCC CAGCAGAGGA GGAAGAGGAA GAGAGCGAAG GCCCTGCTGA GGCCCAGGAG
    CGGAGCTCAA GTCTGGCCCC TGCCCCGTCG CAGACCTTGG AGGCGACTGT GCAAGTTGCT
    GTGTCGGTGC CAAAGAAAAA ACGAAGGATG AAGGAGCTGA ACAAGAAGGA GGCAGTAGGC
    GATTTGCTGG ATGCCTTTAA AGAGTCTCAG ATCAGCGACA GTGCCTCAGA GGTGGAAAAC
    AAGCCTCCCC CTTCTGCCCC TGCCCGAGAA ACAGAGGACG CAGCCCCCAC CCGTCCCCAG
    GAGGAGTCGG AGGAGACGTG GGAGGAGAAG GAGGACAAGC TGGCCCCAGA GAAGGGCAAG
    GCTGGGGACC AGAAGTACCG CTACAAGGAA GAGCAGTGGA AGCCATTGAA TCCTGAGGAG
    AAGAAGCGGT ATGACCGGGA GTTCCTGCTG GGCTTCCAGT TCATCTTTGC CAGCATGCAG
    AAACCTGAGG GGTTGCCCCA GATCACGGAT GTGGTGCTGG ACAAGCCCTG TGTACCTTCG
    CAGGCCAACA AGACCCCATT GCGGGCGCTC GACCCCATCC GCCTCAGCGG CATGAACTGC
    AGCCCTGACT TCACCCCCTC CTTTGCCAAC CTTGGGCGGC CTGTCATGGG CAACCGGGGC
    CTGCCTTCAG GGTTGGGTCC TCGCCGCTCA CAGCAGACTC AGAGGAAGGA GCCCCGTAAA
    ATCATTGCCA CTGTGTCCCT CAATGAGGAT GTCAAGTTGA ACAAGGCTGA GAAGGCCTGG
    AAACCCAGCA GCAAGCGTGC CTCTGAGGAG GAGGATCCGG AGAACATCAA GACACAGGAA
    CTGCTTCGCC GCGTCCGCAG CATCCTCAAC AAGCTGACAC CCCAGATGTT CCAGCAATTG
    ATGAAGCAGG TGATGGAGTT GTCCATCGAC ACGGAGGAGA GACTCAAGGG TGTCATTGAC
    CTTGTCTTCG AGAAAGCCAT CTCGGAGCCA AACTTCTCTG TTGCCTACGC TAACATGTGC
    CGTTGCCTTA TGGGGCTCAA AGTGCCCACG ACAGACAAGC CCACAGTGAC TGTGAACTTC
    CGCAAGCTGC TGCTCAACCG CTGCCAGAAG GAGTTTGAGA AGGACAAGGA TGACGACGAG
    ATCTTTGAGA AGCGGCAGAA AGAAATGGAC GATGCCAGTG CTCCCGAGGA GAAGGCGCGC
    ATGAAGGATG AGCTGGAGGA GGCGCGGGAC AAGGCCCGAC GGCGGTCCCT GGGTAATATC
    AAGTTCATTG GAGAGCTCTT CAAACTGAAG ATGCTGACAG AGGCCATCAT GCATGACTGT
    GTGGTGAAGC TGCTCAAAAA CCACGATGAG GAGTCTCTTG AGTGCCTTTG CCGCCTGCTT
    ACAACCATTG GCAAGGACTT GGACTTTGAG AAGGCCAAGC CCAGAATGGA CCAGTACTTT
    AACCAGATGG AGAAAATCAT CAAGGAGAAG AAGACATCAT CCCGAATCCG TTTTATGCTG
    CAGGATGTGA TTGATCTCAG GCGGAATAGC TGGGTGCCAC GGCGAGGAGA TCAGGGCCCC
    AAAACCATCG ACCAGATCCA CAAAGAGGCG GAGATGGAGG AGCATCGGGA ACACATCAAA
    GTGCAGCAGC TCATGTCAAA AGACAAAAGG AGAGGACCTC CTGGACCATC CATCAGCGGT
    GGACGCAGCA GCCTGGTTGC AGATGACGGC TGGAACACTG TGCCCATCAG CAAGGGCAAC
    CGGCCTATTG ACACCAGCCG GCTAACGAAA ATCACCAAGC CTGGATTGAT CGACTCCAAC
    AACCAGCTCT TTGCGCCAGG CGGGCGTCTG AGCTGGGGCA AAGGCAGTAG TGGAGGGTCT
    GGCGCAAAGC CTGCAGATTC AGCATCTGAT TCAGGGCGAC CGGCCACAAG CACCTTGAAC
    CGCTTCTCAG CACTCCAGCA GTCCACCCCT GCTGAGAGCT CGGAGTCCCG TCGCGTGGTG
    CAGAGGAGCA GCTCCAGCCG CGACAGGTCA GAAAAGGCTG GGGACAGAGG GGACCGGGAG
    TCACGTTCAG AGAAGAGCAG TGACCGTCTG GAGCGTCCTG ATCGGGGAGA GCGGGGAGAG
    AGGAACAGGT CTGCTGTCAC CAAGAGGAGC TTCAGCAAAG AGACAGAGGA CAGGAGCAGA
    GAACGGGAAA AGCAGAGTGG CCCTGAGGCC GTGCGTAAGA CTGCTAGCAT GTCAGAGGAA
    CGGGACAGGA GCCGAGAGAC GATTAAACAA GAGCCAGCAC CTCCTGCGGC ATCACCCAAA
    CCCATGCTGT CAGAGGAGGA GCTGGAGAAG AAATCTAAGG CGATCATAGA GGAATATCTG
    CACATCAATG ATATGAAGGA GGCCCTGCAG TGTGTGCAGG AGCTGGGCAG CCCCTCCTTG
    CTCTACGTCT TCGTGCGGAA TGGCATTGAG TCCACGCTTG AGCGGAGCAC GATCTCCCGC
    GAGCACATGG GGGTCCTGCT GTGCCACCTG GTGAAGGCTG GCACGCTCTC CAAGGAGCAG
    TACTATAAAG GGCTGCGGGA GATCCTGGAG ATTGCAGAAG ACATGGAGAT TGATATCCCG
    CACATCTGGC TGTATCTTGC CGAGCTCATA ACACCTATCC TGCAAGAGGA AGGCATCCCC
    ATGGAGGAGC TGTTCAGGGA GATAACAAAG CCCCTGGTGC CCCTTGGGAA GGCCACCACG
    CTGCTGGTTG AGGTGCTGGG CTTATTGTGC AAGGGCATGA GCCAGAAGAC TGCGGGCAAG
    CTGTGGCGGG ATGGGGGACT GAGCTGGAAG GAATTCCTGC CTGAGGACCA GGACATCAAC
    AAATTCGTCA CAGAGCAGAA ATTGGAGTAC ACGATGGGGG ACAACTCGGA CACGCCAAGC
    TGCAAGGAGC TGACCTCGGA GGAGCTGTGC AAGCAAATGG ACAAACTGCT GAAGGAGAAC
    TCGAACAACC AAAGAATATA TGACTGGATT GAGGCCAACC TAAGTGAGCA GCAGGTCTCG
    TCCAACACAT TTATCAGGGC CCTGATGACA TCCGTGTGCC ATTCAGCCAT CACCTTTGAG
    AACCCGTACC GTGTAGACGC CCTGGTCATT AGCAACCGGG CCAAGCTGCT GCAGAAGTAC
    ATGCGGGATG AGCAGAAGGA GCTTCAGGCA CTCTACGCCT TGCAGGCTTT GGTGGTGAAG
    TTGGACCAGC CTCCAAACCT GCTGCGGATG TTCTTTGATG CTCTCTACGA TGAGGACGTC
    ATCAAGGAGG AGGCCTTCTA CAAGTGGGAG TCCAGCAAGG ACCCGGCCGA GCAGCAGGGC
    AAAGGGGTGG CCCTGAAATC AGTGACAGCG TTTTTCACCT GGCTCCGGGA AGCAGAGGAC
    GAGTCAGACA ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009429.1
    CDS74..4951
    Translation

    Target ORF information:

    RefSeq Version XM_025153661.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGGTCGT TCCACATACG TTGTCCCAAC ACAGCAGTAC
    CCGGTCCAGC CCGGTGCCCC TAGTTTTTAC CCTGGAGCCA GCCCCACAGA GTTCGGGACT
    TACGCGGGTG CCTATTACCC GGCCCAGGGG GTGCAGCAGT TCCCGGCGGG GGTCCCCACT
    GCCCAGGTCA TTGTGAGCCA GCAGCCACCG ATCCCCCCAA AACGAGAGCG CAAGACGATC
    CGGATACGAG ACCCCAACCA AGGTGGCAAA GACATCACTG AAGAAATTAT GTCCGGAGCA
    AGGACCTCAT CTACCCCCAC CCCTCCCCAG GCTGGAAGCG GTTTGGAACC CCAGGCCAAT
    GGAGAAACCC CTCATGTAGC AGTTATTGTT CGGCCAGATG ACCGCCCAAA GCCCGCGCTG
    GTGGTGAGCA AGCCCGTCTC CCTGGAGCCC AGCAAGTCAG CGTCCCCGTC GCCTCCCCCT
    CCCCTCATCC CCGAGGTGGA GCCTGTGGTG ATGTCAACTA TGACGCTGGT GCCAATGGAG
    CCCCCCATGG AAGCGGACAC TAAAGTGGAG CTGGGCGAGG CGCCGCCCGA CCTGCACCAG
    ACGTTTAGCG CTATCACTAC AGTGCCAGGG GCTGGGGAGC TGCCCCTTGT ACCCCCACCT
    GACATGGACA CAGCGGCTGT GGTGGAGGAG GAGGTGGCAG AGGAGGTGGA AGAGGTTGCC
    ATTCCTCTCC TGGAGCCCAC CTTGCAGGCG CCCGCCCTGC CCGAGGTGCC ATCGGTGCCT
    GCCGCCCCCC TCATGCCAGC CGTGCCCCCG GTGCCAGCTG CGCCGTCGCC GCCGCTCGTT
    GTCCCGCCGG TCCCTGAAGC ACCCGCCAAA CCCGCCTCTC CCAGCCCCCC GCCGCCCCAG
    GAAGAGCCCT GCCCTGAGCC TGTTGCTCAG CCTGCTGCTG AGGCCAATGG GGTCTTAGAG
    GAGGTGCCCC AGCCGCTCCC TGAGGCGCCC GTGTGCCAGC CGGTGCCTGT GCCCATATCG
    GAGCCTGCCC CGGTGCCCGC CCCAGCGCCC ACTCTGGACT CTCCCATTGC GCAGCCTGAG
    GAGCTGCCCT TGCCCAACGG GATGGAGGGC TCCAGCAAAG TGGAGGCGAG CGAGGAGCAG
    CCTGAGTCGG ACATCAGCCC CATCTCGGAG CCCGAGGAGC CAGTGCAGCC TGGAACCCCC
    GCCTCCCCCC CAGCAGAGGA GGAAGAGGAA GAGAGCGAAG GCCCTGCTGA GGCCCAGGAG
    CGGAGCTCAA GTCTGGCCCC TGCCCCGTCG CAGACCTTGG AGGCGACTGT GCAAGTTGCT
    GTGTCGGTGC CAAAGAAAAA ACGAAGGATG AAGGAGCTGA ACAAGAAGGA GGCAGTAGGC
    GATTTGCTGG ATGCCTTTAA AGAGTCTCAG ATCAGCGACA GTGCCTCAGA GGTGGAAAAC
    AAGCCTCCCC CTTCTGCCCC TGCCCGAGAA ACAGAGGACG CAGCCCCCAC CCGTCCCCAG
    GAGGAGTCGG AGGAGACGTG GGAGGAGAAG GAGGACAAGC TGGCCCCAGA GAAGGGCAAG
    GCTGGGGACC AGAAGTACCG CTACAAGGAA GAGCAGTGGA AGCCATTGAA TCCTGAGGAG
    AAGAAGCGGT ATGACCGGGA GTTCCTGCTG GGCTTCCAGT TCATCTTTGC CAGCATGCAG
    AAACCTGAGG GGTTGCCCCA GATCACGGAT GTGGTGCTGG ACAAGCCCTG TGTACCTTCG
    CAGGCCAACA AGACCCCATT GCGGGCGCTC GACCCCATCC GCCTCAGCGG CATGAACTGC
    AGCCCTGACT TCACCCCCTC CTTTGCCAAC CTTGGGCGGC CTGTCATGGG CAACCGGGGC
    CTGCCTTCAG GGTTGGGTCC TCGCCGCTCA CAGCAGACTC AGAGGAAGGA GCCCCGTAAA
    ATCATTGCCA CTGTGTCCCT CAATGAGGAT GTCAAGTTGA ACAAGGCTGA GAAGGCCTGG
    AAACCCAGCA GCAAGCGTGC CTCTGAGGAG GAGGATCCGG AGAACATCAA GACACAGGAA
    CTGCTTCGCC GCGTCCGCAG CATCCTCAAC AAGCTGACAC CCCAGATGTT CCAGCAATTG
    ATGAAGCAGG TGATGGAGTT GTCCATCGAC ACGGAGGAGA GACTCAAGGG TGTCATTGAC
    CTTGTCTTCG AGAAAGCCAT CTCGGAGCCA AACTTCTCTG TTGCCTACGC TAACATGTGC
    CGTTGCCTTA TGGGGCTCAA AGTGCCCACG ACAGACAAGC CCACAGTGAC TGTGAACTTC
    CGCAAGCTGC TGCTCAACCG CTGCCAGAAG GAGTTTGAGA AGGACAAGGA TGACGACGAG
    ATCTTTGAGA AGCGGCAGAA AGAAATGGAC GATGCCAGTG CTCCCGAGGA GAAGGCGCGC
    ATGAAGGATG AGCTGGAGGA GGCGCGGGAC AAGGCCCGAC GGCGGTCCCT GGGTAATATC
    AAGTTCATTG GAGAGCTCTT CAAACTGAAG ATGCTGACAG AGGCCATCAT GCATGACTGT
    GTGGTGAAGC TGCTCAAAAA CCACGATGAG GAGTCTCTTG AGTGCCTTTG CCGCCTGCTT
    ACAACCATTG GCAAGGACTT GGACTTTGAG AAGGCCAAGC CCAGAATGGA CCAGTACTTT
    AACCAGATGG AGAAAATCAT CAAGGAGAAG AAGACATCAT CCCGAATCCG TTTTATGCTG
    CAGGATGTGA TTGATCTCAG GCGGAATAGC TGGGTGCCAC GGCGAGGAGA TCAGGGCCCC
    AAAACCATCG ACCAGATCCA CAAAGAGGCG GAGATGGAGG AGCATCGGGA ACACATCAAA
    GTGCAGCAGC TCATGTCAAA AGACAAAAGG AGAGGACCTC CTGGACCATC CATCAGCGGT
    GGACGCAGCA GCCTGGTTGC AGATGACGGC TGGAACACTG TGCCCATCAG CAAGGGCAAC
    CGGCCTATTG ACACCAGCCG GCTAACGAAA ATCACCAAGC CTGGATTGAT CGACTCCAAC
    AACCAGCTCT TTGCGCCAGG CGGGCGTCTG AGCTGGGGCA AAGGCAGTAG TGGAGGGTCT
    GGCGCAAAGC CTGCAGATTC AGCATCTGAT TCAGGGCGAC CGGCCACAAG CACCTTGAAC
    CGCTTCTCAG CACTCCAGCA GTCCACCCCT GCTGAGAGCT CGGAGTCCCG TCGCGTGGTG
    CAGAGGAGCA GCTCCAGCCG CGACAGGTCA GAAAAGGCTG GGGACAGAGG GGACCGGGAG
    TCACGTTCAG AGAAGAGCAG TGACCGTCTG GAGCGTCCTG ATCGGGGAGA GCGGGGAGAG
    AGGAACAGGT CTGCTGTCAC CAAGAGGAGC TTCAGCAAAG AGACAGAGGA CAGGAGCAGA
    GAACGGGAAA AGCAGAGTGG CCCTGAGGCC GTGCGTAAGA CTGCTAGCAT GTCAGAGGAA
    CGGGACAGGA GCCGAGAGAC GATTAAACAA GAGCCAGCAC CTCCTGCGGC ATCACCCAAA
    CCCATGCTGT CAGAGGAGGA GCTGGAGAAG AAATCTAAGG CGATCATAGA GGAATATCTG
    CACATCAATG ATATGAAGGA GGCCCTGCAG TGTGTGCAGG AGCTGGGCAG CCCCTCCTTG
    CTCTACGTCT TCGTGCGGAA TGGCATTGAG TCCACGCTTG AGCGGAGCAC GATCTCCCGC
    GAGCACATGG GGGTCCTGCT GTGCCACCTG GTGAAGGCTG GCACGCTCTC CAAGGAGCAG
    TACTATAAAG GGCTGCGGGA GATCCTGGAG ATTGCAGAAG ACATGGAGAT TGATATCCCG
    CACATCTGGC TGTATCTTGC CGAGCTCATA ACACCTATCC TGCAAGAGGA AGGCATCCCC
    ATGGAGGAGC TGTTCAGGGA GATAACAAAG CCCCTGGTGC CCCTTGGGAA GGCCACCACG
    CTGCTGGTTG AGGTGCTGGG CTTATTGTGC AAGGGCATGA GCCAGAAGAC TGCGGGCAAG
    CTGTGGCGGG ATGGGGGACT GAGCTGGAAG GAATTCCTGC CTGAGGACCA GGACATCAAC
    AAATTCGTCA CAGAGCAGAA ATTGGAGTAC ACGATGGGGG ACAACTCGGA CACGCCAAGC
    TGCAAGGAGC TGACCTCGGA GGAGCTGTGC AAGCAAATGG ACAAACTGCT GAAGGAGAAC
    TCGAACAACC AAAGAATATA TGACTGGATT GAGGCCAACC TAAGTGAGCA GCAGGTCTCG
    TCCAACACAT TTATCAGGGC CCTGATGACA TCCGTGTGCC ATTCAGCCAT CACCTTTGAG
    AACCCGTACC GTGTAGACGC CCTGGTCATT AGCAACCGGG CCAAGCTGCT GCAGAAGTAC
    ATGCGGGATG AGCAGAAGGA GCTTCAGGCA CTCTACGCCT TGCAGGCTTT GGTGGTGAAG
    TTGGACCAGC CTCCAAACCT GCTGCGGATG TTCTTTGATG CTCTCTACGA TGAGGACGTC
    ATCAAGGAGG AGGCCTTCTA CAAGTGGGAG TCCAGCAAGG ACCCGGCCGA GCAGCAGGGC
    AAAGGGGTGG CCCTGAAATC AGTGACAGCG TTTTTCACCT GGCTCCGGGA AGCAGAGGAC
    GAGTCAGACA ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152825
    Clone ID Related Accession (Same CDS sequence) XM_025153662.1
    Accession Version XM_025153662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009430.1
    CDS73..4896
    Translation

    Target ORF information:

    RefSeq Version XM_025153662.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147022.1
    CDS135..5078
    Translation

    Target ORF information:

    RefSeq Version XM_015291536.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291528.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147014.1
    CDS103..5046
    Translation

    Target ORF information:

    RefSeq Version XM_015291528.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291528.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291528.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147014.1
    CDS73..5016
    Translation

    Target ORF information:

    RefSeq Version XM_015291528.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291534.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147020.1
    CDS188..5131
    Translation

    Target ORF information:

    RefSeq Version XM_015291534.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291534.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_025153664.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009432.1
    CDS181..5124
    Translation

    Target ORF information:

    RefSeq Version XM_025153664.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153664.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291535.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147021.1
    CDS136..5079
    Translation

    Target ORF information:

    RefSeq Version XM_015291535.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291536.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147022.1
    CDS133..5076
    Translation

    Target ORF information:

    RefSeq Version XM_015291536.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147021.1
    CDS161..5104
    Translation

    Target ORF information:

    RefSeq Version XM_015291535.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39989
    Clone ID Related Accession (Same CDS sequence) XM_015291536.2 , XM_015291528.2 , XM_015291528.1 , XM_015291534.2 , XM_025153664.1 , XM_015291535.1 , XM_015291536.1 , XM_015291535.2 , XM_015291534.1
    Accession Version XM_015291534.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147020.1
    CDS185..5128
    Translation

    Target ORF information:

    RefSeq Version XM_015291534.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGCA TCTGATTCAG GGCGACCGGC CACAAGCACC
    TTGAACCGCT TCTCAGCACT CCAGCAGTCC ACCCCTGCTG AGAGCTCGGA GTCCCGTCGC
    GTGGTGCAGA GGAGCAGCTC CAGCCGCGAC AGGTCAGAAA AGGCTGGGGA CAGAGGGGAC
    CGGGAGTCAC GTTCAGAGAA GAGCAGTGAC CGTCTGGAGC GTCCTGATCG GGGAGAGCGG
    GGAGAGAGGA ACAGGTCTGC TGTCACCAAG AGGAGCTTCA GCAAAGAGAC AGAGGACAGG
    AGCAGAGAAC GGGAAAAGCA GAGTGGCCCT GAGGCCGTGC GTAAGACTGC TAGCATGTCA
    GAGGAACGGG ACAGGAGCCG AGAGACGATT AAACAAGAGC CAGCACCTCC TGCGGCATCA
    CCCAAACCCA TGCTGTCAGA GGAGGAGCTG GAGAAGAAAT CTAAGGCGAT CATAGAGGAA
    TATCTGCACA TCAATGATAT GAAGGAGGCC CTGCAGTGTG TGCAGGAGCT GGGCAGCCCC
    TCCTTGCTCT ACGTCTTCGT GCGGAATGGC ATTGAGTCCA CGCTTGAGCG GAGCACGATC
    TCCCGCGAGC ACATGGGGGT CCTGCTGTGC CACCTGGTGA AGGCTGGCAC GCTCTCCAAG
    GAGCAGTACT ATAAAGGGCT GCGGGAGATC CTGGAGATTG CAGAAGACAT GGAGATTGAT
    ATCCCGCACA TCTGGCTGTA TCTTGCCGAG CTCATAACAC CTATCCTGCA AGAGGAAGGC
    ATCCCCATGG AGGAGCTGTT CAGGGAGATA ACAAAGCCCC TGGTGCCCCT TGGGAAGGCC
    ACCACGCTGC TGGTTGAGGT GCTGGGCTTA TTGTGCAAGG GCATGAGCCA GAAGACTGCG
    GGCAAGCTGT GGCGGGATGG GGGACTGAGC TGGAAGGAAT TCCTGCCTGA GGACCAGGAC
    ATCAACAAAT TCGTCACAGA GCAGAAATTG GAGTACACGA TGGGGGACAA CTCGGACACG
    CCAAGCTGCA AGGAGCTGAC CTCGGAGGAG CTGTGCAAGC AAATGGACAA ACTGCTGAAG
    GAGAACTCGA ACAACCAAAG AATATATGAC TGGATTGAGG CCAACCTAAG TGAGCAGCAG
    GTCTCGTCCA ACACATTTAT CAGGGCCCTG ATGACATCCG TGTGCCATTC AGCCATCACC
    TTTGAGAACC CGTACCGTGT AGACGCCCTG GTCATTAGCA ACCGGGCCAA GCTGCTGCAG
    AAGTACATGC GGGATGAGCA GAAGGAGCTT CAGGCACTCT ACGCCTTGCA GGCTTTGGTG
    GTGAAGTTGG ACCAGCCTCC AAACCTGCTG CGGATGTTCT TTGATGCTCT CTACGATGAG
    GACGTCATCA AGGAGGAGGC CTTCTACAAG TGGGAGTCCA GCAAGGACCC GGCCGAGCAG
    CAGGGCAAAG GGGTGGCCCT GAAATCAGTG ACAGCGTTTT TCACCTGGCT CCGGGAAGCA
    GAGGACGAGT CAGACAACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39990
    Clone ID Related Accession (Same CDS sequence) XM_015291529.1 , XM_015291529.2
    Accession Version XM_015291529.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGGG CGACCGGCCA CAAGCACCTT GAACCGCTTC
    TCAGCACTCC AGCAGTCCAC CCCTGCTGAG AGCTCGGAGT CCCGTCGCGT GGTGCAGAGG
    AGCAGCTCCA GCCGCGACAG GTCAGAAAAG GCTGGGGACA GAGGGGACCG GGAGTCACGT
    TCAGAGAAGA GCAGTGACCG TCTGGAGCGT CCTGATCGGG GAGAGCGGGG AGAGAGGAAC
    AGGTCTGCTG TCACCAAGAG GAGCTTCAGC AAAGAGACAG AGGACAGGAG CAGAGAACGG
    GAAAAGCAGA GTGGCCCTGA GGCCGTGCGT AAGACTGCTA GCATGTCAGA GGAACGGGAC
    AGGAGCCGAG AGACGATTAA ACAAGAGCCA GCACCTCCTG CGGCATCACC CAAACCCATG
    CTGTCAGAGG AGGAGCTGGA GAAGAAATCT AAGGCGATCA TAGAGGAATA TCTGCACATC
    AATGATATGA AGGAGGCCCT GCAGTGTGTG CAGGAGCTGG GCAGCCCCTC CTTGCTCTAC
    GTCTTCGTGC GGAATGGCAT TGAGTCCACG CTTGAGCGGA GCACGATCTC CCGCGAGCAC
    ATGGGGGTCC TGCTGTGCCA CCTGGTGAAG GCTGGCACGC TCTCCAAGGA GCAGTACTAT
    AAAGGGCTGC GGGAGATCCT GGAGATTGCA GAAGACATGG AGATTGATAT CCCGCACATC
    TGGCTGTATC TTGCCGAGCT CATAACACCT ATCCTGCAAG AGGAAGGCAT CCCCATGGAG
    GAGCTGTTCA GGGAGATAAC AAAGCCCCTG GTGCCCCTTG GGAAGGCCAC CACGCTGCTG
    GTTGAGGTGC TGGGCTTATT GTGCAAGGGC ATGAGCCAGA AGACTGCGGG CAAGCTGTGG
    CGGGATGGGG GACTGAGCTG GAAGGAATTC CTGCCTGAGG ACCAGGACAT CAACAAATTC
    GTCACAGAGC AGAAATTGGA GTACACGATG GGGGACAACT CGGACACGCC AAGCTGCAAG
    GAGCTGACCT CGGAGGAGCT GTGCAAGCAA ATGGACAAAC TGCTGAAGGA GAACTCGAAC
    AACCAAAGAA TATATGACTG GATTGAGGCC AACCTAAGTG AGCAGCAGGT CTCGTCCAAC
    ACATTTATCA GGGCCCTGAT GACATCCGTG TGCCATTCAG CCATCACCTT TGAGAACCCG
    TACCGTGTAG ACGCCCTGGT CATTAGCAAC CGGGCCAAGC TGCTGCAGAA GTACATGCGG
    GATGAGCAGA AGGAGCTTCA GGCACTCTAC GCCTTGCAGG CTTTGGTGGT GAAGTTGGAC
    CAGCCTCCAA ACCTGCTGCG GATGTTCTTT GATGCTCTCT ACGATGAGGA CGTCATCAAG
    GAGGAGGCCT TCTACAAGTG GGAGTCCAGC AAGGACCCGG CCGAGCAGCA GGGCAAAGGG
    GTGGCCCTGA AATCAGTGAC AGCGTTTTTC ACCTGGCTCC GGGAAGCAGA GGACGAGTCA
    GACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147015.1
    CDS74..5005
    Translation

    Target ORF information:

    RefSeq Version XM_015291529.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGGG CGACCGGCCA CAAGCACCTT GAACCGCTTC
    TCAGCACTCC AGCAGTCCAC CCCTGCTGAG AGCTCGGAGT CCCGTCGCGT GGTGCAGAGG
    AGCAGCTCCA GCCGCGACAG GTCAGAAAAG GCTGGGGACA GAGGGGACCG GGAGTCACGT
    TCAGAGAAGA GCAGTGACCG TCTGGAGCGT CCTGATCGGG GAGAGCGGGG AGAGAGGAAC
    AGGTCTGCTG TCACCAAGAG GAGCTTCAGC AAAGAGACAG AGGACAGGAG CAGAGAACGG
    GAAAAGCAGA GTGGCCCTGA GGCCGTGCGT AAGACTGCTA GCATGTCAGA GGAACGGGAC
    AGGAGCCGAG AGACGATTAA ACAAGAGCCA GCACCTCCTG CGGCATCACC CAAACCCATG
    CTGTCAGAGG AGGAGCTGGA GAAGAAATCT AAGGCGATCA TAGAGGAATA TCTGCACATC
    AATGATATGA AGGAGGCCCT GCAGTGTGTG CAGGAGCTGG GCAGCCCCTC CTTGCTCTAC
    GTCTTCGTGC GGAATGGCAT TGAGTCCACG CTTGAGCGGA GCACGATCTC CCGCGAGCAC
    ATGGGGGTCC TGCTGTGCCA CCTGGTGAAG GCTGGCACGC TCTCCAAGGA GCAGTACTAT
    AAAGGGCTGC GGGAGATCCT GGAGATTGCA GAAGACATGG AGATTGATAT CCCGCACATC
    TGGCTGTATC TTGCCGAGCT CATAACACCT ATCCTGCAAG AGGAAGGCAT CCCCATGGAG
    GAGCTGTTCA GGGAGATAAC AAAGCCCCTG GTGCCCCTTG GGAAGGCCAC CACGCTGCTG
    GTTGAGGTGC TGGGCTTATT GTGCAAGGGC ATGAGCCAGA AGACTGCGGG CAAGCTGTGG
    CGGGATGGGG GACTGAGCTG GAAGGAATTC CTGCCTGAGG ACCAGGACAT CAACAAATTC
    GTCACAGAGC AGAAATTGGA GTACACGATG GGGGACAACT CGGACACGCC AAGCTGCAAG
    GAGCTGACCT CGGAGGAGCT GTGCAAGCAA ATGGACAAAC TGCTGAAGGA GAACTCGAAC
    AACCAAAGAA TATATGACTG GATTGAGGCC AACCTAAGTG AGCAGCAGGT CTCGTCCAAC
    ACATTTATCA GGGCCCTGAT GACATCCGTG TGCCATTCAG CCATCACCTT TGAGAACCCG
    TACCGTGTAG ACGCCCTGGT CATTAGCAAC CGGGCCAAGC TGCTGCAGAA GTACATGCGG
    GATGAGCAGA AGGAGCTTCA GGCACTCTAC GCCTTGCAGG CTTTGGTGGT GAAGTTGGAC
    CAGCCTCCAA ACCTGCTGCG GATGTTCTTT GATGCTCTCT ACGATGAGGA CGTCATCAAG
    GAGGAGGCCT TCTACAAGTG GGAGTCCAGC AAGGACCCGG CCGAGCAGCA GGGCAAAGGG
    GTGGCCCTGA AATCAGTGAC AGCGTTTTTC ACCTGGCTCC GGGAAGCAGA GGACGAGTCA
    GACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39990
    Clone ID Related Accession (Same CDS sequence) XM_015291529.1 , XM_015291529.2
    Accession Version XM_015291529.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGGG CGACCGGCCA CAAGCACCTT GAACCGCTTC
    TCAGCACTCC AGCAGTCCAC CCCTGCTGAG AGCTCGGAGT CCCGTCGCGT GGTGCAGAGG
    AGCAGCTCCA GCCGCGACAG GTCAGAAAAG GCTGGGGACA GAGGGGACCG GGAGTCACGT
    TCAGAGAAGA GCAGTGACCG TCTGGAGCGT CCTGATCGGG GAGAGCGGGG AGAGAGGAAC
    AGGTCTGCTG TCACCAAGAG GAGCTTCAGC AAAGAGACAG AGGACAGGAG CAGAGAACGG
    GAAAAGCAGA GTGGCCCTGA GGCCGTGCGT AAGACTGCTA GCATGTCAGA GGAACGGGAC
    AGGAGCCGAG AGACGATTAA ACAAGAGCCA GCACCTCCTG CGGCATCACC CAAACCCATG
    CTGTCAGAGG AGGAGCTGGA GAAGAAATCT AAGGCGATCA TAGAGGAATA TCTGCACATC
    AATGATATGA AGGAGGCCCT GCAGTGTGTG CAGGAGCTGG GCAGCCCCTC CTTGCTCTAC
    GTCTTCGTGC GGAATGGCAT TGAGTCCACG CTTGAGCGGA GCACGATCTC CCGCGAGCAC
    ATGGGGGTCC TGCTGTGCCA CCTGGTGAAG GCTGGCACGC TCTCCAAGGA GCAGTACTAT
    AAAGGGCTGC GGGAGATCCT GGAGATTGCA GAAGACATGG AGATTGATAT CCCGCACATC
    TGGCTGTATC TTGCCGAGCT CATAACACCT ATCCTGCAAG AGGAAGGCAT CCCCATGGAG
    GAGCTGTTCA GGGAGATAAC AAAGCCCCTG GTGCCCCTTG GGAAGGCCAC CACGCTGCTG
    GTTGAGGTGC TGGGCTTATT GTGCAAGGGC ATGAGCCAGA AGACTGCGGG CAAGCTGTGG
    CGGGATGGGG GACTGAGCTG GAAGGAATTC CTGCCTGAGG ACCAGGACAT CAACAAATTC
    GTCACAGAGC AGAAATTGGA GTACACGATG GGGGACAACT CGGACACGCC AAGCTGCAAG
    GAGCTGACCT CGGAGGAGCT GTGCAAGCAA ATGGACAAAC TGCTGAAGGA GAACTCGAAC
    AACCAAAGAA TATATGACTG GATTGAGGCC AACCTAAGTG AGCAGCAGGT CTCGTCCAAC
    ACATTTATCA GGGCCCTGAT GACATCCGTG TGCCATTCAG CCATCACCTT TGAGAACCCG
    TACCGTGTAG ACGCCCTGGT CATTAGCAAC CGGGCCAAGC TGCTGCAGAA GTACATGCGG
    GATGAGCAGA AGGAGCTTCA GGCACTCTAC GCCTTGCAGG CTTTGGTGGT GAAGTTGGAC
    CAGCCTCCAA ACCTGCTGCG GATGTTCTTT GATGCTCTCT ACGATGAGGA CGTCATCAAG
    GAGGAGGCCT TCTACAAGTG GGAGTCCAGC AAGGACCCGG CCGAGCAGCA GGGCAAAGGG
    GTGGCCCTGA AATCAGTGAC AGCGTTTTTC ACCTGGCTCC GGGAAGCAGA GGACGAGTCA
    GACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147015.1
    CDS75..5006
    Translation

    Target ORF information:

    RefSeq Version XM_015291529.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291529.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GCCCTGTGTA
    CCTTCGCAGG CCAACAAGAC CCCATTGCGG GCGCTCGACC CCATCCGCCT CAGCGGCATG
    AACTGCAGCC CTGACTTCAC CCCCTCCTTT GCCAACCTTG GGCGGCCTGT CATGGGCAAC
    CGGGGCCTGC CTTCAGGGTT GGGTCCTCGC CGCTCACAGC AGACTCAGAG GAAGGAGCCC
    CGTAAAATCA TTGCCACTGT GTCCCTCAAT GAGGATGTCA AGTTGAACAA GGCTGAGAAG
    GCCTGGAAAC CCAGCAGCAA GCGTGCCTCT GAGGAGGAGG ATCCGGAGAA CATCAAGACA
    CAGGAACTGC TTCGCCGCGT CCGCAGCATC CTCAACAAGC TGACACCCCA GATGTTCCAG
    CAATTGATGA AGCAGGTGAT GGAGTTGTCC ATCGACACGG AGGAGAGACT CAAGGGTGTC
    ATTGACCTTG TCTTCGAGAA AGCCATCTCG GAGCCAAACT TCTCTGTTGC CTACGCTAAC
    ATGTGCCGTT GCCTTATGGG GCTCAAAGTG CCCACGACAG ACAAGCCCAC AGTGACTGTG
    AACTTCCGCA AGCTGCTGCT CAACCGCTGC CAGAAGGAGT TTGAGAAGGA CAAGGATGAC
    GACGAGATCT TTGAGAAGCG GCAGAAAGAA ATGGACGATG CCAGTGCTCC CGAGGAGAAG
    GCGCGCATGA AGGATGAGCT GGAGGAGGCG CGGGACAAGG CCCGACGGCG GTCCCTGGGT
    AATATCAAGT TCATTGGAGA GCTCTTCAAA CTGAAGATGC TGACAGAGGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT CAAAAACCAC GATGAGGAGT CTCTTGAGTG CCTTTGCCGC
    CTGCTTACAA CCATTGGCAA GGACTTGGAC TTTGAGAAGG CCAAGCCCAG AATGGACCAG
    TACTTTAACC AGATGGAGAA AATCATCAAG GAGAAGAAGA CATCATCCCG AATCCGTTTT
    ATGCTGCAGG ATGTGATTGA TCTCAGGCGG AATAGCTGGG TGCCACGGCG AGGAGATCAG
    GGCCCCAAAA CCATCGACCA GATCCACAAA GAGGCGGAGA TGGAGGAGCA TCGGGAACAC
    ATCAAAGTGC AGCAGCTCAT GTCAAAAGAC AAAAGGAGAG GACCTCCTGG ACCATCCATC
    AGCGGTGGAC GCAGCAGCCT GGTTGCAGAT GACGGCTGGA ACACTGTGCC CATCAGCAAG
    GGCAACCGGC CTATTGACAC CAGCCGGCTA ACGAAAATCA CCAAGCCTGG ATTGATCGAC
    TCCAACAACC AGCTCTTTGC GCCAGGCGGG CGTCTGAGCT GGGGCAAAGG CAGTAGTGGA
    GGGTCTGGCG CAAAGCCTGC AGATTCAGGG CGACCGGCCA CAAGCACCTT GAACCGCTTC
    TCAGCACTCC AGCAGTCCAC CCCTGCTGAG AGCTCGGAGT CCCGTCGCGT GGTGCAGAGG
    AGCAGCTCCA GCCGCGACAG GTCAGAAAAG GCTGGGGACA GAGGGGACCG GGAGTCACGT
    TCAGAGAAGA GCAGTGACCG TCTGGAGCGT CCTGATCGGG GAGAGCGGGG AGAGAGGAAC
    AGGTCTGCTG TCACCAAGAG GAGCTTCAGC AAAGAGACAG AGGACAGGAG CAGAGAACGG
    GAAAAGCAGA GTGGCCCTGA GGCCGTGCGT AAGACTGCTA GCATGTCAGA GGAACGGGAC
    AGGAGCCGAG AGACGATTAA ACAAGAGCCA GCACCTCCTG CGGCATCACC CAAACCCATG
    CTGTCAGAGG AGGAGCTGGA GAAGAAATCT AAGGCGATCA TAGAGGAATA TCTGCACATC
    AATGATATGA AGGAGGCCCT GCAGTGTGTG CAGGAGCTGG GCAGCCCCTC CTTGCTCTAC
    GTCTTCGTGC GGAATGGCAT TGAGTCCACG CTTGAGCGGA GCACGATCTC CCGCGAGCAC
    ATGGGGGTCC TGCTGTGCCA CCTGGTGAAG GCTGGCACGC TCTCCAAGGA GCAGTACTAT
    AAAGGGCTGC GGGAGATCCT GGAGATTGCA GAAGACATGG AGATTGATAT CCCGCACATC
    TGGCTGTATC TTGCCGAGCT CATAACACCT ATCCTGCAAG AGGAAGGCAT CCCCATGGAG
    GAGCTGTTCA GGGAGATAAC AAAGCCCCTG GTGCCCCTTG GGAAGGCCAC CACGCTGCTG
    GTTGAGGTGC TGGGCTTATT GTGCAAGGGC ATGAGCCAGA AGACTGCGGG CAAGCTGTGG
    CGGGATGGGG GACTGAGCTG GAAGGAATTC CTGCCTGAGG ACCAGGACAT CAACAAATTC
    GTCACAGAGC AGAAATTGGA GTACACGATG GGGGACAACT CGGACACGCC AAGCTGCAAG
    GAGCTGACCT CGGAGGAGCT GTGCAAGCAA ATGGACAAAC TGCTGAAGGA GAACTCGAAC
    AACCAAAGAA TATATGACTG GATTGAGGCC AACCTAAGTG AGCAGCAGGT CTCGTCCAAC
    ACATTTATCA GGGCCCTGAT GACATCCGTG TGCCATTCAG CCATCACCTT TGAGAACCCG
    TACCGTGTAG ACGCCCTGGT CATTAGCAAC CGGGCCAAGC TGCTGCAGAA GTACATGCGG
    GATGAGCAGA AGGAGCTTCA GGCACTCTAC GCCTTGCAGG CTTTGGTGGT GAAGTTGGAC
    CAGCCTCCAA ACCTGCTGCG GATGTTCTTT GATGCTCTCT ACGATGAGGA CGTCATCAAG
    GAGGAGGCCT TCTACAAGTG GGAGTCCAGC AAGGACCCGG CCGAGCAGCA GGGCAAAGGG
    GTGGCCCTGA AATCAGTGAC AGCGTTTTTC ACCTGGCTCC GGGAAGCAGA GGACGAGTCA
    GACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39991
    Clone ID Related Accession (Same CDS sequence) XM_015291530.2 , XM_015291530.1
    Accession Version XM_015291530.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GGCCAACAAG
    ACCCCATTGC GGGCGCTCGA CCCCATCCGC CTCAGCGGCA TGAACTGCAG CCCTGACTTC
    ACCCCCTCCT TTGCCAACCT TGGGCGGCCT GTCATGGGCA ACCGGGGCCT GCCTTCAGGG
    TTGGGTCCTC GCCGCTCACA GCAGACTCAG AGGAAGGAGC CCCGTAAAAT CATTGCCACT
    GTGTCCCTCA ATGAGGATGT CAAGTTGAAC AAGGCTGAGA AGGCCTGGAA ACCCAGCAGC
    AAGCGTGCCT CTGAGGAGGA GGATCCGGAG AACATCAAGA CACAGGAACT GCTTCGCCGC
    GTCCGCAGCA TCCTCAACAA GCTGACACCC CAGATGTTCC AGCAATTGAT GAAGCAGGTG
    ATGGAGTTGT CCATCGACAC GGAGGAGAGA CTCAAGGGTG TCATTGACCT TGTCTTCGAG
    AAAGCCATCT CGGAGCCAAA CTTCTCTGTT GCCTACGCTA ACATGTGCCG TTGCCTTATG
    GGGCTCAAAG TGCCCACGAC AGACAAGCCC ACAGTGACTG TGAACTTCCG CAAGCTGCTG
    CTCAACCGCT GCCAGAAGGA GTTTGAGAAG GACAAGGATG ACGACGAGAT CTTTGAGAAG
    CGGCAGAAAG AAATGGACGA TGCCAGTGCT CCCGAGGAGA AGGCGCGCAT GAAGGATGAG
    CTGGAGGAGG CGCGGGACAA GGCCCGACGG CGGTCCCTGG GTAATATCAA GTTCATTGGA
    GAGCTCTTCA AACTGAAGAT GCTGACAGAG GCCATCATGC ATGACTGTGT GGTGAAGCTG
    CTCAAAAACC ACGATGAGGA GTCTCTTGAG TGCCTTTGCC GCCTGCTTAC AACCATTGGC
    AAGGACTTGG ACTTTGAGAA GGCCAAGCCC AGAATGGACC AGTACTTTAA CCAGATGGAG
    AAAATCATCA AGGAGAAGAA GACATCATCC CGAATCCGTT TTATGCTGCA GGATGTGATT
    GATCTCAGGC GGAATAGCTG GGTGCCACGG CGAGGAGATC AGGGCCCCAA AACCATCGAC
    CAGATCCACA AAGAGGCGGA GATGGAGGAG CATCGGGAAC ACATCAAAGT GCAGCAGCTC
    ATGTCAAAAG ACAAAAGGAG AGGACCTCCT GGACCATCCA TCAGCGGTGG ACGCAGCAGC
    CTGGTTGCAG ATGACGGCTG GAACACTGTG CCCATCAGCA AGGGCAACCG GCCTATTGAC
    ACCAGCCGGC TAACGAAAAT CACCAAGCCT GGATTGATCG ACTCCAACAA CCAGCTCTTT
    GCGCCAGGCG GGCGTCTGAG CTGGGGCAAA GGCAGTAGTG GAGGGTCTGG CGCAAAGCCT
    GCAGATTCAG CATCTGATTC AGGGCGACCG GCCACAAGCA CCTTGAACCG CTTCTCAGCA
    CTCCAGCAGT CCACCCCTGC TGAGAGCTCG GAGTCCCGTC GCGTGGTGCA GAGGAGCAGC
    TCCAGCCGCG ACAGGTCAGA AAAGGCTGGG GACAGAGGGG ACCGGGAGTC ACGTTCAGAG
    AAGAGCAGTG ACCGTCTGGA GCGTCCTGAT CGGGGAGAGC GGGGAGAGAG GAACAGGTCT
    GCTGTCACCA AGAGGAGCTT CAGCAAAGAG ACAGAGGACA GGAGCAGAGA ACGGGAAAAG
    CAGAGTGGCC CTGAGGCCGT GCGTAAGACT GCTAGCATGT CAGAGGAACG GGACAGGAGC
    CGAGAGACGA TTAAACAAGA GCCAGCACCT CCTGCGGCAT CACCCAAACC CATGCTGTCA
    GAGGAGGAGC TGGAGAAGAA ATCTAAGGCG ATCATAGAGG AATATCTGCA CATCAATGAT
    ATGAAGGAGG CCCTGCAGTG TGTGCAGGAG CTGGGCAGCC CCTCCTTGCT CTACGTCTTC
    GTGCGGAATG GCATTGAGTC CACGCTTGAG CGGAGCACGA TCTCCCGCGA GCACATGGGG
    GTCCTGCTGT GCCACCTGGT GAAGGCTGGC ACGCTCTCCA AGGAGCAGTA CTATAAAGGG
    CTGCGGGAGA TCCTGGAGAT TGCAGAAGAC ATGGAGATTG ATATCCCGCA CATCTGGCTG
    TATCTTGCCG AGCTCATAAC ACCTATCCTG CAAGAGGAAG GCATCCCCAT GGAGGAGCTG
    TTCAGGGAGA TAACAAAGCC CCTGGTGCCC CTTGGGAAGG CCACCACGCT GCTGGTTGAG
    GTGCTGGGCT TATTGTGCAA GGGCATGAGC CAGAAGACTG CGGGCAAGCT GTGGCGGGAT
    GGGGGACTGA GCTGGAAGGA ATTCCTGCCT GAGGACCAGG ACATCAACAA ATTCGTCACA
    GAGCAGAAAT TGGAGTACAC GATGGGGGAC AACTCGGACA CGCCAAGCTG CAAGGAGCTG
    ACCTCGGAGG AGCTGTGCAA GCAAATGGAC AAACTGCTGA AGGAGAACTC GAACAACCAA
    AGAATATATG ACTGGATTGA GGCCAACCTA AGTGAGCAGC AGGTCTCGTC CAACACATTT
    ATCAGGGCCC TGATGACATC CGTGTGCCAT TCAGCCATCA CCTTTGAGAA CCCGTACCGT
    GTAGACGCCC TGGTCATTAG CAACCGGGCC AAGCTGCTGC AGAAGTACAT GCGGGATGAG
    CAGAAGGAGC TTCAGGCACT CTACGCCTTG CAGGCTTTGG TGGTGAAGTT GGACCAGCCT
    CCAAACCTGC TGCGGATGTT CTTTGATGCT CTCTACGATG AGGACGTCAT CAAGGAGGAG
    GCCTTCTACA AGTGGGAGTC CAGCAAGGAC CCGGCCGAGC AGCAGGGCAA AGGGGTGGCC
    CTGAAATCAG TGACAGCGTT TTTCACCTGG CTCCGGGAAG CAGAGGACGA GTCAGACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147016.1
    CDS75..5000
    Translation

    Target ORF information:

    RefSeq Version XM_015291530.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291530.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GGCCAACAAG
    ACCCCATTGC GGGCGCTCGA CCCCATCCGC CTCAGCGGCA TGAACTGCAG CCCTGACTTC
    ACCCCCTCCT TTGCCAACCT TGGGCGGCCT GTCATGGGCA ACCGGGGCCT GCCTTCAGGG
    TTGGGTCCTC GCCGCTCACA GCAGACTCAG AGGAAGGAGC CCCGTAAAAT CATTGCCACT
    GTGTCCCTCA ATGAGGATGT CAAGTTGAAC AAGGCTGAGA AGGCCTGGAA ACCCAGCAGC
    AAGCGTGCCT CTGAGGAGGA GGATCCGGAG AACATCAAGA CACAGGAACT GCTTCGCCGC
    GTCCGCAGCA TCCTCAACAA GCTGACACCC CAGATGTTCC AGCAATTGAT GAAGCAGGTG
    ATGGAGTTGT CCATCGACAC GGAGGAGAGA CTCAAGGGTG TCATTGACCT TGTCTTCGAG
    AAAGCCATCT CGGAGCCAAA CTTCTCTGTT GCCTACGCTA ACATGTGCCG TTGCCTTATG
    GGGCTCAAAG TGCCCACGAC AGACAAGCCC ACAGTGACTG TGAACTTCCG CAAGCTGCTG
    CTCAACCGCT GCCAGAAGGA GTTTGAGAAG GACAAGGATG ACGACGAGAT CTTTGAGAAG
    CGGCAGAAAG AAATGGACGA TGCCAGTGCT CCCGAGGAGA AGGCGCGCAT GAAGGATGAG
    CTGGAGGAGG CGCGGGACAA GGCCCGACGG CGGTCCCTGG GTAATATCAA GTTCATTGGA
    GAGCTCTTCA AACTGAAGAT GCTGACAGAG GCCATCATGC ATGACTGTGT GGTGAAGCTG
    CTCAAAAACC ACGATGAGGA GTCTCTTGAG TGCCTTTGCC GCCTGCTTAC AACCATTGGC
    AAGGACTTGG ACTTTGAGAA GGCCAAGCCC AGAATGGACC AGTACTTTAA CCAGATGGAG
    AAAATCATCA AGGAGAAGAA GACATCATCC CGAATCCGTT TTATGCTGCA GGATGTGATT
    GATCTCAGGC GGAATAGCTG GGTGCCACGG CGAGGAGATC AGGGCCCCAA AACCATCGAC
    CAGATCCACA AAGAGGCGGA GATGGAGGAG CATCGGGAAC ACATCAAAGT GCAGCAGCTC
    ATGTCAAAAG ACAAAAGGAG AGGACCTCCT GGACCATCCA TCAGCGGTGG ACGCAGCAGC
    CTGGTTGCAG ATGACGGCTG GAACACTGTG CCCATCAGCA AGGGCAACCG GCCTATTGAC
    ACCAGCCGGC TAACGAAAAT CACCAAGCCT GGATTGATCG ACTCCAACAA CCAGCTCTTT
    GCGCCAGGCG GGCGTCTGAG CTGGGGCAAA GGCAGTAGTG GAGGGTCTGG CGCAAAGCCT
    GCAGATTCAG CATCTGATTC AGGGCGACCG GCCACAAGCA CCTTGAACCG CTTCTCAGCA
    CTCCAGCAGT CCACCCCTGC TGAGAGCTCG GAGTCCCGTC GCGTGGTGCA GAGGAGCAGC
    TCCAGCCGCG ACAGGTCAGA AAAGGCTGGG GACAGAGGGG ACCGGGAGTC ACGTTCAGAG
    AAGAGCAGTG ACCGTCTGGA GCGTCCTGAT CGGGGAGAGC GGGGAGAGAG GAACAGGTCT
    GCTGTCACCA AGAGGAGCTT CAGCAAAGAG ACAGAGGACA GGAGCAGAGA ACGGGAAAAG
    CAGAGTGGCC CTGAGGCCGT GCGTAAGACT GCTAGCATGT CAGAGGAACG GGACAGGAGC
    CGAGAGACGA TTAAACAAGA GCCAGCACCT CCTGCGGCAT CACCCAAACC CATGCTGTCA
    GAGGAGGAGC TGGAGAAGAA ATCTAAGGCG ATCATAGAGG AATATCTGCA CATCAATGAT
    ATGAAGGAGG CCCTGCAGTG TGTGCAGGAG CTGGGCAGCC CCTCCTTGCT CTACGTCTTC
    GTGCGGAATG GCATTGAGTC CACGCTTGAG CGGAGCACGA TCTCCCGCGA GCACATGGGG
    GTCCTGCTGT GCCACCTGGT GAAGGCTGGC ACGCTCTCCA AGGAGCAGTA CTATAAAGGG
    CTGCGGGAGA TCCTGGAGAT TGCAGAAGAC ATGGAGATTG ATATCCCGCA CATCTGGCTG
    TATCTTGCCG AGCTCATAAC ACCTATCCTG CAAGAGGAAG GCATCCCCAT GGAGGAGCTG
    TTCAGGGAGA TAACAAAGCC CCTGGTGCCC CTTGGGAAGG CCACCACGCT GCTGGTTGAG
    GTGCTGGGCT TATTGTGCAA GGGCATGAGC CAGAAGACTG CGGGCAAGCT GTGGCGGGAT
    GGGGGACTGA GCTGGAAGGA ATTCCTGCCT GAGGACCAGG ACATCAACAA ATTCGTCACA
    GAGCAGAAAT TGGAGTACAC GATGGGGGAC AACTCGGACA CGCCAAGCTG CAAGGAGCTG
    ACCTCGGAGG AGCTGTGCAA GCAAATGGAC AAACTGCTGA AGGAGAACTC GAACAACCAA
    AGAATATATG ACTGGATTGA GGCCAACCTA AGTGAGCAGC AGGTCTCGTC CAACACATTT
    ATCAGGGCCC TGATGACATC CGTGTGCCAT TCAGCCATCA CCTTTGAGAA CCCGTACCGT
    GTAGACGCCC TGGTCATTAG CAACCGGGCC AAGCTGCTGC AGAAGTACAT GCGGGATGAG
    CAGAAGGAGC TTCAGGCACT CTACGCCTTG CAGGCTTTGG TGGTGAAGTT GGACCAGCCT
    CCAAACCTGC TGCGGATGTT CTTTGATGCT CTCTACGATG AGGACGTCAT CAAGGAGGAG
    GCCTTCTACA AGTGGGAGTC CAGCAAGGAC CCGGCCGAGC AGCAGGGCAA AGGGGTGGCC
    CTGAAATCAG TGACAGCGTT TTTCACCTGG CTCCGGGAAG CAGAGGACGA GTCAGACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39991
    Clone ID Related Accession (Same CDS sequence) XM_015291530.2 , XM_015291530.1
    Accession Version XM_015291530.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GGCCAACAAG
    ACCCCATTGC GGGCGCTCGA CCCCATCCGC CTCAGCGGCA TGAACTGCAG CCCTGACTTC
    ACCCCCTCCT TTGCCAACCT TGGGCGGCCT GTCATGGGCA ACCGGGGCCT GCCTTCAGGG
    TTGGGTCCTC GCCGCTCACA GCAGACTCAG AGGAAGGAGC CCCGTAAAAT CATTGCCACT
    GTGTCCCTCA ATGAGGATGT CAAGTTGAAC AAGGCTGAGA AGGCCTGGAA ACCCAGCAGC
    AAGCGTGCCT CTGAGGAGGA GGATCCGGAG AACATCAAGA CACAGGAACT GCTTCGCCGC
    GTCCGCAGCA TCCTCAACAA GCTGACACCC CAGATGTTCC AGCAATTGAT GAAGCAGGTG
    ATGGAGTTGT CCATCGACAC GGAGGAGAGA CTCAAGGGTG TCATTGACCT TGTCTTCGAG
    AAAGCCATCT CGGAGCCAAA CTTCTCTGTT GCCTACGCTA ACATGTGCCG TTGCCTTATG
    GGGCTCAAAG TGCCCACGAC AGACAAGCCC ACAGTGACTG TGAACTTCCG CAAGCTGCTG
    CTCAACCGCT GCCAGAAGGA GTTTGAGAAG GACAAGGATG ACGACGAGAT CTTTGAGAAG
    CGGCAGAAAG AAATGGACGA TGCCAGTGCT CCCGAGGAGA AGGCGCGCAT GAAGGATGAG
    CTGGAGGAGG CGCGGGACAA GGCCCGACGG CGGTCCCTGG GTAATATCAA GTTCATTGGA
    GAGCTCTTCA AACTGAAGAT GCTGACAGAG GCCATCATGC ATGACTGTGT GGTGAAGCTG
    CTCAAAAACC ACGATGAGGA GTCTCTTGAG TGCCTTTGCC GCCTGCTTAC AACCATTGGC
    AAGGACTTGG ACTTTGAGAA GGCCAAGCCC AGAATGGACC AGTACTTTAA CCAGATGGAG
    AAAATCATCA AGGAGAAGAA GACATCATCC CGAATCCGTT TTATGCTGCA GGATGTGATT
    GATCTCAGGC GGAATAGCTG GGTGCCACGG CGAGGAGATC AGGGCCCCAA AACCATCGAC
    CAGATCCACA AAGAGGCGGA GATGGAGGAG CATCGGGAAC ACATCAAAGT GCAGCAGCTC
    ATGTCAAAAG ACAAAAGGAG AGGACCTCCT GGACCATCCA TCAGCGGTGG ACGCAGCAGC
    CTGGTTGCAG ATGACGGCTG GAACACTGTG CCCATCAGCA AGGGCAACCG GCCTATTGAC
    ACCAGCCGGC TAACGAAAAT CACCAAGCCT GGATTGATCG ACTCCAACAA CCAGCTCTTT
    GCGCCAGGCG GGCGTCTGAG CTGGGGCAAA GGCAGTAGTG GAGGGTCTGG CGCAAAGCCT
    GCAGATTCAG CATCTGATTC AGGGCGACCG GCCACAAGCA CCTTGAACCG CTTCTCAGCA
    CTCCAGCAGT CCACCCCTGC TGAGAGCTCG GAGTCCCGTC GCGTGGTGCA GAGGAGCAGC
    TCCAGCCGCG ACAGGTCAGA AAAGGCTGGG GACAGAGGGG ACCGGGAGTC ACGTTCAGAG
    AAGAGCAGTG ACCGTCTGGA GCGTCCTGAT CGGGGAGAGC GGGGAGAGAG GAACAGGTCT
    GCTGTCACCA AGAGGAGCTT CAGCAAAGAG ACAGAGGACA GGAGCAGAGA ACGGGAAAAG
    CAGAGTGGCC CTGAGGCCGT GCGTAAGACT GCTAGCATGT CAGAGGAACG GGACAGGAGC
    CGAGAGACGA TTAAACAAGA GCCAGCACCT CCTGCGGCAT CACCCAAACC CATGCTGTCA
    GAGGAGGAGC TGGAGAAGAA ATCTAAGGCG ATCATAGAGG AATATCTGCA CATCAATGAT
    ATGAAGGAGG CCCTGCAGTG TGTGCAGGAG CTGGGCAGCC CCTCCTTGCT CTACGTCTTC
    GTGCGGAATG GCATTGAGTC CACGCTTGAG CGGAGCACGA TCTCCCGCGA GCACATGGGG
    GTCCTGCTGT GCCACCTGGT GAAGGCTGGC ACGCTCTCCA AGGAGCAGTA CTATAAAGGG
    CTGCGGGAGA TCCTGGAGAT TGCAGAAGAC ATGGAGATTG ATATCCCGCA CATCTGGCTG
    TATCTTGCCG AGCTCATAAC ACCTATCCTG CAAGAGGAAG GCATCCCCAT GGAGGAGCTG
    TTCAGGGAGA TAACAAAGCC CCTGGTGCCC CTTGGGAAGG CCACCACGCT GCTGGTTGAG
    GTGCTGGGCT TATTGTGCAA GGGCATGAGC CAGAAGACTG CGGGCAAGCT GTGGCGGGAT
    GGGGGACTGA GCTGGAAGGA ATTCCTGCCT GAGGACCAGG ACATCAACAA ATTCGTCACA
    GAGCAGAAAT TGGAGTACAC GATGGGGGAC AACTCGGACA CGCCAAGCTG CAAGGAGCTG
    ACCTCGGAGG AGCTGTGCAA GCAAATGGAC AAACTGCTGA AGGAGAACTC GAACAACCAA
    AGAATATATG ACTGGATTGA GGCCAACCTA AGTGAGCAGC AGGTCTCGTC CAACACATTT
    ATCAGGGCCC TGATGACATC CGTGTGCCAT TCAGCCATCA CCTTTGAGAA CCCGTACCGT
    GTAGACGCCC TGGTCATTAG CAACCGGGCC AAGCTGCTGC AGAAGTACAT GCGGGATGAG
    CAGAAGGAGC TTCAGGCACT CTACGCCTTG CAGGCTTTGG TGGTGAAGTT GGACCAGCCT
    CCAAACCTGC TGCGGATGTT CTTTGATGCT CTCTACGATG AGGACGTCAT CAAGGAGGAG
    GCCTTCTACA AGTGGGAGTC CAGCAAGGAC CCGGCCGAGC AGCAGGGCAA AGGGGTGGCC
    CTGAAATCAG TGACAGCGTT TTTCACCTGG CTCCGGGAAG CAGAGGACGA GTCAGACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147016.1
    CDS74..4999
    Translation

    Target ORF information:

    RefSeq Version XM_015291530.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGGGAGG ATTCAGGTCT CTCCAGCATT TCTACCAGAA CAGGGCCCAG
    CCTCCCGCCA GTGCATCCCG CGTGCAGAGC AACACAACGG CCCGGCCTGG CCCTCCTGCC
    CATGTGTATC CAGCTGCTTC CCAGGTGATG ATGATACCCT CTCAGATATC CTACACACCT
    TCCCAAGGAG CCTATTACAT TCCCGGACAG GGTCGTTCCA CATACGTTGT CCCAACACAG
    CAGTACCCGG TCCAGCCCGG TGCCCCTAGT TTTTACCCTG GAGCCAGCCC CACAGAGTTC
    GGGACTTACG CGGGTGCCTA TTACCCGGCC CAGGGGGTGC AGCAGTTCCC GGCGGGGGTC
    CCCACTGCCC AGGTCATTGT GAGCCAGCAG CCACCGATCC CCCCAAAACG AGAGCGCAAG
    ACGATCCGGA TACGAGACCC CAACCAAGGT GGCAAAGACA TCACTGAAGA AATTATGTCC
    GGAGCAAGGA CCTCATCTAC CCCCACCCCT CCCCAGGCTG GAAGCGGTTT GGAACCCCAG
    GCCAATGGAG AAACCCCTCA TGTAGCAGTT ATTGTTCGGC CAGATGACCG CCCAAAGCCC
    GCGCTGGTGG TGAGCAAGCC CGTCTCCCTG GAGCCCAGCA AGTCAGCGTC CCCGTCGCCT
    CCCCCTCCCC TCATCCCCGA GGTGGAGCCT GTGGTGATGT CAACTATGAC GCTGGTGCCA
    ATGGAGCCCC CCATGGAAGC GGACACTAAA GTGGAGCTGG GCGAGGCGCC GCCCGACCTG
    CACCAGACGT TTAGCGCTAT CACTACAGTG CCAGGGGCTG GGGAGCTGCC CCTTGTACCC
    CCACCTGACA TGGACACAGC GGCTGTGGTG GAGGAGGAGG TGGCAGAGGA GGTGGAAGAG
    GTTGCCATTC CTCTCCTGGA GCCCACCTTG CAGGCGCCCG CCCTGCCCGA GGTGCCATCG
    GTGCCTGCCG CCCCCCTCAT GCCAGCCGTG CCCCCGGTGC CAGCTGCGCC GTCGCCGCCG
    CTCGTTGTCC CGCCGGTCCC TGAAGCACCC GCCAAACCCG CCTCTCCCAG CCCCCCGCCG
    CCCCAGGAAG AGCCCTGCCC TGAGCCTGTT GCTCAGCCTG CTGCTGAGGC CAATGGGGTC
    TTAGAGGAGG TGCCCCAGCC GCTCCCTGAG GCGCCCGTGT GCCAGCCGGT GCCTGTGCCC
    ATATCGGAGC CTGCCCCGGT GCCCGCCCCA GCGCCCACTC TGGACTCTCC CATTGCGCAG
    CCTGAGGAGC TGCCCTTGCC CAACGGGATG GAGGGCTCCA GCAAAGTGGA GGCGAGCGAG
    GAGCAGCCTG AGTCGGACAT CAGCCCCATC TCGGAGCCCG AGGAGCCAGT GCAGCCTGGA
    ACCCCCGCCT CCCCCCCAGC AGAGGAGGAA GAGGAAGAGA GCGAAGGCCC TGCTGAGGCC
    CAGGAGCGGA GCTCAAGTCT GGCCCCTGCC CCGTCGCAGA CCTTGGAGGC GACTGTGCAA
    GTTGCTGTGT CGGTGCCAAA GAAAAAACGA AGGATGAAGG AGCTGAACAA GAAGGAGGCA
    GTAGGCGATT TGCTGGATGC CTTTAAAGAG TCTCAGATCA GCGACAGTGC CTCAGAGGTG
    GAAAACAAGC CTCCCCCTTC TGCCCCTGCC CGAGAAACAG AGGACGCAGC CCCCACCCGT
    CCCCAGGAGG AGTCGGAGGA GACGTGGGAG GAGAAGGAGG ACAAGCTGGC CCCAGAGAAG
    GGCAAGGCTG GGGACCAGAA GTACCGCTAC AAGGAAGAGC AGTGGAAGCC ATTGAATCCT
    GAGGAGAAGA AGCGGTATGA CCGGGAGTTC CTGCTGGGCT TCCAGTTCAT CTTTGCCAGC
    ATGCAGAAAC CTGAGGGGTT GCCCCAGATC ACGGATGTGG TGCTGGACAA GGCCAACAAG
    ACCCCATTGC GGGCGCTCGA CCCCATCCGC CTCAGCGGCA TGAACTGCAG CCCTGACTTC
    ACCCCCTCCT TTGCCAACCT TGGGCGGCCT GTCATGGGCA ACCGGGGCCT GCCTTCAGGG
    TTGGGTCCTC GCCGCTCACA GCAGACTCAG AGGAAGGAGC CCCGTAAAAT CATTGCCACT
    GTGTCCCTCA ATGAGGATGT CAAGTTGAAC AAGGCTGAGA AGGCCTGGAA ACCCAGCAGC
    AAGCGTGCCT CTGAGGAGGA GGATCCGGAG AACATCAAGA CACAGGAACT GCTTCGCCGC
    GTCCGCAGCA TCCTCAACAA GCTGACACCC CAGATGTTCC AGCAATTGAT GAAGCAGGTG
    ATGGAGTTGT CCATCGACAC GGAGGAGAGA CTCAAGGGTG TCATTGACCT TGTCTTCGAG
    AAAGCCATCT CGGAGCCAAA CTTCTCTGTT GCCTACGCTA ACATGTGCCG TTGCCTTATG
    GGGCTCAAAG TGCCCACGAC AGACAAGCCC ACAGTGACTG TGAACTTCCG CAAGCTGCTG
    CTCAACCGCT GCCAGAAGGA GTTTGAGAAG GACAAGGATG ACGACGAGAT CTTTGAGAAG
    CGGCAGAAAG AAATGGACGA TGCCAGTGCT CCCGAGGAGA AGGCGCGCAT GAAGGATGAG
    CTGGAGGAGG CGCGGGACAA GGCCCGACGG CGGTCCCTGG GTAATATCAA GTTCATTGGA
    GAGCTCTTCA AACTGAAGAT GCTGACAGAG GCCATCATGC ATGACTGTGT GGTGAAGCTG
    CTCAAAAACC ACGATGAGGA GTCTCTTGAG TGCCTTTGCC GCCTGCTTAC AACCATTGGC
    AAGGACTTGG ACTTTGAGAA GGCCAAGCCC AGAATGGACC AGTACTTTAA CCAGATGGAG
    AAAATCATCA AGGAGAAGAA GACATCATCC CGAATCCGTT TTATGCTGCA GGATGTGATT
    GATCTCAGGC GGAATAGCTG GGTGCCACGG CGAGGAGATC AGGGCCCCAA AACCATCGAC
    CAGATCCACA AAGAGGCGGA GATGGAGGAG CATCGGGAAC ACATCAAAGT GCAGCAGCTC
    ATGTCAAAAG ACAAAAGGAG AGGACCTCCT GGACCATCCA TCAGCGGTGG ACGCAGCAGC
    CTGGTTGCAG ATGACGGCTG GAACACTGTG CCCATCAGCA AGGGCAACCG GCCTATTGAC
    ACCAGCCGGC TAACGAAAAT CACCAAGCCT GGATTGATCG ACTCCAACAA CCAGCTCTTT
    GCGCCAGGCG GGCGTCTGAG CTGGGGCAAA GGCAGTAGTG GAGGGTCTGG CGCAAAGCCT
    GCAGATTCAG CATCTGATTC AGGGCGACCG GCCACAAGCA CCTTGAACCG CTTCTCAGCA
    CTCCAGCAGT CCACCCCTGC TGAGAGCTCG GAGTCCCGTC GCGTGGTGCA GAGGAGCAGC
    TCCAGCCGCG ACAGGTCAGA AAAGGCTGGG GACAGAGGGG ACCGGGAGTC ACGTTCAGAG
    AAGAGCAGTG ACCGTCTGGA GCGTCCTGAT CGGGGAGAGC GGGGAGAGAG GAACAGGTCT
    GCTGTCACCA AGAGGAGCTT CAGCAAAGAG ACAGAGGACA GGAGCAGAGA ACGGGAAAAG
    CAGAGTGGCC CTGAGGCCGT GCGTAAGACT GCTAGCATGT CAGAGGAACG GGACAGGAGC
    CGAGAGACGA TTAAACAAGA GCCAGCACCT CCTGCGGCAT CACCCAAACC CATGCTGTCA
    GAGGAGGAGC TGGAGAAGAA ATCTAAGGCG ATCATAGAGG AATATCTGCA CATCAATGAT
    ATGAAGGAGG CCCTGCAGTG TGTGCAGGAG CTGGGCAGCC CCTCCTTGCT CTACGTCTTC
    GTGCGGAATG GCATTGAGTC CACGCTTGAG CGGAGCACGA TCTCCCGCGA GCACATGGGG
    GTCCTGCTGT GCCACCTGGT GAAGGCTGGC ACGCTCTCCA AGGAGCAGTA CTATAAAGGG
    CTGCGGGAGA TCCTGGAGAT TGCAGAAGAC ATGGAGATTG ATATCCCGCA CATCTGGCTG
    TATCTTGCCG AGCTCATAAC ACCTATCCTG CAAGAGGAAG GCATCCCCAT GGAGGAGCTG
    TTCAGGGAGA TAACAAAGCC CCTGGTGCCC CTTGGGAAGG CCACCACGCT GCTGGTTGAG
    GTGCTGGGCT TATTGTGCAA GGGCATGAGC CAGAAGACTG CGGGCAAGCT GTGGCGGGAT
    GGGGGACTGA GCTGGAAGGA ATTCCTGCCT GAGGACCAGG ACATCAACAA ATTCGTCACA
    GAGCAGAAAT TGGAGTACAC GATGGGGGAC AACTCGGACA CGCCAAGCTG CAAGGAGCTG
    ACCTCGGAGG AGCTGTGCAA GCAAATGGAC AAACTGCTGA AGGAGAACTC GAACAACCAA
    AGAATATATG ACTGGATTGA GGCCAACCTA AGTGAGCAGC AGGTCTCGTC CAACACATTT
    ATCAGGGCCC TGATGACATC CGTGTGCCAT TCAGCCATCA CCTTTGAGAA CCCGTACCGT
    GTAGACGCCC TGGTCATTAG CAACCGGGCC AAGCTGCTGC AGAAGTACAT GCGGGATGAG
    CAGAAGGAGC TTCAGGCACT CTACGCCTTG CAGGCTTTGG TGGTGAAGTT GGACCAGCCT
    CCAAACCTGC TGCGGATGTT CTTTGATGCT CTCTACGATG AGGACGTCAT CAAGGAGGAG
    GCCTTCTACA AGTGGGAGTC CAGCAAGGAC CCGGCCGAGC AGCAGGGCAA AGGGGTGGCC
    CTGAAATCAG TGACAGCGTT TTTCACCTGG CTCCGGGAAG CAGAGGACGA GTCAGACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39992
    Clone ID Related Accession (Same CDS sequence) XM_015291531.2 , XM_015291531.1
    Accession Version XM_015291531.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147017.1
    CDS74..4996
    Translation

    Target ORF information:

    RefSeq Version XM_015291531.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39992
    Clone ID Related Accession (Same CDS sequence) XM_015291531.2 , XM_015291531.1
    Accession Version XM_015291531.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147017.1
    CDS73..4995
    Translation

    Target ORF information:

    RefSeq Version XM_015291531.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGTTTCCAGC AGGGCCTCGT
    GCTATTCACC AGCAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291532.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147018.1
    CDS72..4961
    Translation

    Target ORF information:

    RefSeq Version XM_015291532.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291538.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147024.1
    CDS174..5063
    Translation

    Target ORF information:

    RefSeq Version XM_015291538.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291539.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147025.1
    CDS135..5024
    Translation

    Target ORF information:

    RefSeq Version XM_015291539.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291539.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291537.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147023.1
    CDS185..5074
    Translation

    Target ORF information:

    RefSeq Version XM_015291537.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147018.1
    CDS73..4962
    Translation

    Target ORF information:

    RefSeq Version XM_015291532.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291539.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147025.1
    CDS132..5021
    Translation

    Target ORF information:

    RefSeq Version XM_015291539.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147024.1
    CDS174..5063
    Translation

    Target ORF information:

    RefSeq Version XM_015291538.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39993
    Clone ID Related Accession (Same CDS sequence) XM_015291532.1 , XM_015291538.1 , XM_015291539.2 , XM_015291537.1 , XM_015291532.2 , XM_015291539.1 , XM_015291538.2 , XM_015291537.2
    Accession Version XM_015291537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147023.1
    CDS188..5077
    Translation

    Target ORF information:

    RefSeq Version XM_015291537.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGCCC
    TGTGTACCTT CGCAGGCCAA CAAGACCCCA TTGCGGGCGC TCGACCCCAT CCGCCTCAGC
    GGCATGAACT GCAGCCCTGA CTTCACCCCC TCCTTTGCCA ACCTTGGGCG GCCTGTCATG
    GGCAACCGGG GCCTGCCTTC AGGGTTGGGT CCTCGCCGCT CACAGCAGAC TCAGAGGAAG
    GAGCCCCGTA AAATCATTGC CACTGTGTCC CTCAATGAGG ATGTCAAGTT GAACAAGGCT
    GAGAAGGCCT GGAAACCCAG CAGCAAGCGT GCCTCTGAGG AGGAGGATCC GGAGAACATC
    AAGACACAGG AACTGCTTCG CCGCGTCCGC AGCATCCTCA ACAAGCTGAC ACCCCAGATG
    TTCCAGCAAT TGATGAAGCA GGTGATGGAG TTGTCCATCG ACACGGAGGA GAGACTCAAG
    GGTGTCATTG ACCTTGTCTT CGAGAAAGCC ATCTCGGAGC CAAACTTCTC TGTTGCCTAC
    GCTAACATGT GCCGTTGCCT TATGGGGCTC AAAGTGCCCA CGACAGACAA GCCCACAGTG
    ACTGTGAACT TCCGCAAGCT GCTGCTCAAC CGCTGCCAGA AGGAGTTTGA GAAGGACAAG
    GATGACGACG AGATCTTTGA GAAGCGGCAG AAAGAAATGG ACGATGCCAG TGCTCCCGAG
    GAGAAGGCGC GCATGAAGGA TGAGCTGGAG GAGGCGCGGG ACAAGGCCCG ACGGCGGTCC
    CTGGGTAATA TCAAGTTCAT TGGAGAGCTC TTCAAACTGA AGATGCTGAC AGAGGCCATC
    ATGCATGACT GTGTGGTGAA GCTGCTCAAA AACCACGATG AGGAGTCTCT TGAGTGCCTT
    TGCCGCCTGC TTACAACCAT TGGCAAGGAC TTGGACTTTG AGAAGGCCAA GCCCAGAATG
    GACCAGTACT TTAACCAGAT GGAGAAAATC ATCAAGGAGA AGAAGACATC ATCCCGAATC
    CGTTTTATGC TGCAGGATGT GATTGATCTC AGGCGGAATA GCTGGGTGCC ACGGCGAGGA
    GATCAGGGCC CCAAAACCAT CGACCAGATC CACAAAGAGG CGGAGATGGA GGAGCATCGG
    GAACACATCA AAGTGCAGCA GCTCATGTCA AAAGACAAAA GGAGAGGACC TCCTGGACCA
    TCCATCAGCG GTGGACGCAG CAGCCTGGTT GCAGATGACG GCTGGAACAC TGTGCCCATC
    AGCAAGGGCA ACCGGCCTAT TGACACCAGC CGGCTAACGA AAATCACCAA GCCTGGATTG
    ATCGACTCCA ACAACCAGCT CTTTGCGCCA GGCGGGCGTC TGAGCTGGGG CAAAGGCAGT
    AGTGGAGGGT CTGGCGCAAA GCCTGCAGAT TCAGCATCTG ATTCAGGGCG ACCGGCCACA
    AGCACCTTGA ACCGCTTCTC AGCACTCCAG CAGTCCACCC CTGCTGAGAG CTCGGAGTCC
    CGTCGCGTGG TGCAGAGGAG CAGCTCCAGC CGCGACAGGT CAGAAAAGGC TGGGGACAGA
    GGGGACCGGG AGTCACGTTC AGAGAAGAGC AGTGACCGTC TGGAGCGTCC TGATCGGGGA
    GAGCGGGGAG AGAGGAACAG GTCTGCTGTC ACCAAGAGGA GCTTCAGCAA AGAGACAGAG
    GACAGGAGCA GAGAACGGGA AAAGCAGAGT GGCCCTGAGG CCGTGCGTAA GACTGCTAGC
    ATGTCAGAGG AACGGGACAG GAGCCGAGAG ACGATTAAAC AAGAGCCAGC ACCTCCTGCG
    GCATCACCCA AACCCATGCT GTCAGAGGAG GAGCTGGAGA AGAAATCTAA GGCGATCATA
    GAGGAATATC TGCACATCAA TGATATGAAG GAGGCCCTGC AGTGTGTGCA GGAGCTGGGC
    AGCCCCTCCT TGCTCTACGT CTTCGTGCGG AATGGCATTG AGTCCACGCT TGAGCGGAGC
    ACGATCTCCC GCGAGCACAT GGGGGTCCTG CTGTGCCACC TGGTGAAGGC TGGCACGCTC
    TCCAAGGAGC AGTACTATAA AGGGCTGCGG GAGATCCTGG AGATTGCAGA AGACATGGAG
    ATTGATATCC CGCACATCTG GCTGTATCTT GCCGAGCTCA TAACACCTAT CCTGCAAGAG
    GAAGGCATCC CCATGGAGGA GCTGTTCAGG GAGATAACAA AGCCCCTGGT GCCCCTTGGG
    AAGGCCACCA CGCTGCTGGT TGAGGTGCTG GGCTTATTGT GCAAGGGCAT GAGCCAGAAG
    ACTGCGGGCA AGCTGTGGCG GGATGGGGGA CTGAGCTGGA AGGAATTCCT GCCTGAGGAC
    CAGGACATCA ACAAATTCGT CACAGAGCAG AAATTGGAGT ACACGATGGG GGACAACTCG
    GACACGCCAA GCTGCAAGGA GCTGACCTCG GAGGAGCTGT GCAAGCAAAT GGACAAACTG
    CTGAAGGAGA ACTCGAACAA CCAAAGAATA TATGACTGGA TTGAGGCCAA CCTAAGTGAG
    CAGCAGGTCT CGTCCAACAC ATTTATCAGG GCCCTGATGA CATCCGTGTG CCATTCAGCC
    ATCACCTTTG AGAACCCGTA CCGTGTAGAC GCCCTGGTCA TTAGCAACCG GGCCAAGCTG
    CTGCAGAAGT ACATGCGGGA TGAGCAGAAG GAGCTTCAGG CACTCTACGC CTTGCAGGCT
    TTGGTGGTGA AGTTGGACCA GCCTCCAAAC CTGCTGCGGA TGTTCTTTGA TGCTCTCTAC
    GATGAGGACG TCATCAAGGA GGAGGCCTTC TACAAGTGGG AGTCCAGCAA GGACCCGGCC
    GAGCAGCAGG GCAAAGGGGT GGCCCTGAAA TCAGTGACAG CGTTTTTCAC CTGGCTCCGG
    GAAGCAGAGG ACGAGTCAGA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39994
    Clone ID Related Accession (Same CDS sequence) XM_015291533.1 , XM_015291533.2 , XM_025153666.1 , XM_025153665.1
    Accession Version XM_015291533.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147019.1
    CDS73..4875
    Translation

    Target ORF information:

    RefSeq Version XM_015291533.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291533.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39994
    Clone ID Related Accession (Same CDS sequence) XM_015291533.1 , XM_015291533.2 , XM_025153666.1 , XM_025153665.1
    Accession Version XM_015291533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147019.1
    CDS73..4875
    Translation

    Target ORF information:

    RefSeq Version XM_015291533.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39994
    Clone ID Related Accession (Same CDS sequence) XM_015291533.1 , XM_015291533.2 , XM_025153666.1 , XM_025153665.1
    Accession Version XM_025153666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009434.1
    CDS135..4937
    Translation

    Target ORF information:

    RefSeq Version XM_025153666.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39994
    Clone ID Related Accession (Same CDS sequence) XM_015291533.1 , XM_015291533.2 , XM_025153666.1 , XM_025153665.1
    Accession Version XM_025153665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009433.1
    CDS188..4990
    Translation

    Target ORF information:

    RefSeq Version XM_025153665.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGCATTT CTACCAGAAC AGGGCCCAGC CTCCCGCCAG TGCATCCCGC
    GTGCAGAGCA ACACAACGGC CCGGCCTGGC CCTCCTGCCC ATGTGTATCC AGCTGCTTCC
    CAGGTGATGA TGATACCCTC TCAGATATCC TACACACCTT CCCAAGGAGC CTATTACATT
    CCCGGACAGG GTCGTTCCAC ATACGTTGTC CCAACACAGC AGTACCCGGT CCAGCCCGGT
    GCCCCTAGTT TTTACCCTGG AGCCAGCCCC ACAGAGTTCG GGACTTACGC GGGTGCCTAT
    TACCCGGCCC AGGGGGTGCA GCAGTTCCCG GCGGGGGTCC CCACTGCCCA GGTCATTGTG
    AGCCAGCAGC CACCGATCCC CCCAAAACGA GAGCGCAAGA CGATCCGGAT ACGAGACCCC
    AACCAAGGTG GCAAAGACAT CACTGAAGAA ATTATGTCCG GAGCAAGGAC CTCATCTACC
    CCCACCCCTC CCCAGGCTGG AAGCGGTTTG GAACCCCAGG CCAATGGAGA AACCCCTCAT
    GTAGCAGTTA TTGTTCGGCC AGATGACCGC CCAAAGCCCG CGCTGGTGGT GAGCAAGCCC
    GTCTCCCTGG AGCCCAGCAA GTCAGCGTCC CCGTCGCCTC CCCCTCCCCT CATCCCCGAG
    GTGGAGCCTG TGGTGATGTC AACTATGACG CTGGTGCCAA TGGAGCCCCC CATGGAAGCG
    GACACTAAAG TGGAGCTGGG CGAGGCGCCG CCCGACCTGC ACCAGACGTT TAGCGCTATC
    ACTACAGTGC CAGGGGCTGG GGAGCTGCCC CTTGTACCCC CACCTGACAT GGACACAGCG
    GCTGTGGTGG AGGAGGAGGT GGCAGAGGAG GTGGAAGAGG TTGCCATTCC TCTCCTGGAG
    CCCACCTTGC AGGCGCCCGC CCTGCCCGAG GTGCCATCGG TGCCTGCCGC CCCCCTCATG
    CCAGCCGTGC CCCCGGTGCC AGCTGCGCCG TCGCCGCCGC TCGTTGTCCC GCCGGTCCCT
    GAAGCACCCG CCAAACCCGC CTCTCCCAGC CCCCCGCCGC CCCAGGAAGA GCCCTGCCCT
    GAGCCTGTTG CTCAGCCTGC TGCTGAGGCC AATGGGGTCT TAGAGGAGGT GCCCCAGCCG
    CTCCCTGAGG CGCCCGTGTG CCAGCCGGTG CCTGTGCCCA TATCGGAGCC TGCCCCGGTG
    CCCGCCCCAG CGCCCACTCT GGACTCTCCC ATTGCGCAGC CTGAGGAGCT GCCCTTGCCC
    AACGGGATGG AGGGCTCCAG CAAAGTGGAG GCGAGCGAGG AGCAGCCTGA GTCGGACATC
    AGCCCCATCT CGGAGCCCGA GGAGCCAGTG CAGCCTGGAA CCCCCGCCTC CCCCCCAGCA
    GAGGAGGAAG AGGAAGAGAG CGAAGGCCCT GCTGAGGCCC AGGAGCGGAG CTCAAGTCTG
    GCCCCTGCCC CGTCGCAGAC CTTGGAGGCG ACTGTGCAAG TTGCTGTGTC GGTGCCAAAG
    AAAAAACGAA GGATGAAGGA GCTGAACAAG AAGGAGGCAG TAGGCGATTT GCTGGATGCC
    TTTAAAGAGT CTCAGATCAG CGACAGTGCC TCAGAGGTGG AAAACAAGCC TCCCCCTTCT
    GCCCCTGCCC GAGAAACAGA GGACGCAGCC CCCACCCGTC CCCAGGAGGA GTCGGAGGAG
    ACGTGGGAGG AGAAGGAGGA CAAGCTGGCC CCAGAGAAGG GCAAGGCTGG GGACCAGAAG
    TACCGCTACA AGGAAGAGCA GTGGAAGCCA TTGAATCCTG AGGAGAAGAA GCGGTATGAC
    CGGGAGTTCC TGCTGGGCTT CCAGTTCATC TTTGCCAGCA TGCAGAAACC TGAGGGGTTG
    CCCCAGATCA CGGATGTGGT GCTGGACAAG CCCTGTGTAC CTTCGCAGGC CAACAAGACC
    CCATTGCGGG CGCTCGACCC CATCCGCCTC AGCGGCATGA ACTGCAGCCC TGACTTCACC
    CCCTCCTTTG CCAACCTTGG GCGGCCTGTC ATGGGCAACC GGGGCCTGCC TTCAGGGTTG
    GGTCCTCGCC GCTCACAGCA GACTCAGAGG AAGGAGCCCC GTAAAATCAT TGCCACTGTG
    TCCCTCAATG AGGATGTCAA GTTGAACAAG GCTGAGAAGG CCTGGAAACC CAGCAGCAAG
    CGTGCCTCTG AGGAGGAGGA TCCGGAGAAC ATCAAGACAC AGGAACTGCT TCGCCGCGTC
    CGCAGCATCC TCAACAAGCT GACACCCCAG ATGTTCCAGC AATTGATGAA GCAGGTGATG
    GAGTTGTCCA TCGACACGGA GGAGAGACTC AAGGGTGTCA TTGACCTTGT CTTCGAGAAA
    GCCATCTCGG AGCCAAACTT CTCTGTTGCC TACGCTAACA TGTGCCGTTG CCTTATGGGG
    CTCAAAGTGC CCACGACAGA CAAGCCCACA GTGACTGTGA ACTTCCGCAA GCTGCTGCTC
    AACCGCTGCC AGAAGGAGTT TGAGAAGGAC AAGGATGACG ACGAGATCTT TGAGAAGCGG
    CAGAAAGAAA TGGACGATGC CAGTGCTCCC GAGGAGAAGG CGCGCATGAA GGATGAGCTG
    GAGGAGGCGC GGGACAAGGC CCGACGGCGG TCCCTGGGTA ATATCAAGTT CATTGGAGAG
    CTCTTCAAAC TGAAGATGCT GACAGAGGCC ATCATGCATG ACTGTGTGGT GAAGCTGCTC
    AAAAACCACG ATGAGGAGTC TCTTGAGTGC CTTTGCCGCC TGCTTACAAC CATTGGCAAG
    GACTTGGACT TTGAGAAGGC CAAGCCCAGA ATGGACCAGT ACTTTAACCA GATGGAGAAA
    ATCATCAAGG AGAAGAAGAC ATCATCCCGA ATCCGTTTTA TGCTGCAGGA TGTGATTGAT
    CTCAGGCGGA ATAGCTGGGT GCCACGGCGA GGAGATCAGG GCCCCAAAAC CATCGACCAG
    ATCCACAAAG AGGCGGAGAT GGAGGAGCAT CGGGAACACA TCAAAGTGCA GCAGCTCATG
    TCAAAAGACA AAAGGAGAGG ACCTCCTGGA CCATCCATCA GCGGTGGACG CAGCAGCCTG
    GTTGCAGATG ACGGCTGGAA CACTGTGCCC ATCAGCAAGG GCAACCGGCC TATTGACACC
    AGCCGGCTAA CGAAAATCAC CAAGCCTGGA TTGATCGACT CCAACAACCA GCTCTTTGCG
    CCAGGCGGGC GTCTGAGCTG GGGCAAAGGC AGTAGTGGAG GGTCTGGCGC AAAGCCTGCA
    GATTCAGCAT CTGATTCAGG GCGACCGGCC ACAAGCACCT TGAACCGCTT CTCAGCACTC
    CAGCAGTCCA CCCCTGCTGA GAGCTCGGAG TCCCGTCGCG TGGTGCAGAG GAGCAGCTCC
    AGCCGCGACA GGTCAGAAAA GGCTGGGGAC AGAGGGGACC GGGAGTCACG TTCAGAGAAG
    AGCAGTGACC GTCTGGAGCG TCCTGATCGG GGAGAGCGGG GAGAGAGGAA CAGGTCTGCT
    GTCACCAAGA GGAGCTTCAG CAAAGAGACA GAGGACAGGA GCAGAGAACG GGAAAAGCAG
    AGTGGCCCTG AGGCCGTGCG TAAGACTGCT AGCATGTCAG AGGAACGGGA CAGGAGCCGA
    GAGACGATTA AACAAGAGCC AGCACCTCCT GCGGCATCAC CCAAACCCAT GCTGTCAGAG
    GAGGAGCTGG AGAAGAAATC TAAGGCGATC ATAGAGGAAT ATCTGCACAT CAATGATATG
    AAGGAGGCCC TGCAGTGTGT GCAGGAGCTG GGCAGCCCCT CCTTGCTCTA CGTCTTCGTG
    CGGAATGGCA TTGAGTCCAC GCTTGAGCGG AGCACGATCT CCCGCGAGCA CATGGGGGTC
    CTGCTGTGCC ACCTGGTGAA GGCTGGCACG CTCTCCAAGG AGCAGTACTA TAAAGGGCTG
    CGGGAGATCC TGGAGATTGC AGAAGACATG GAGATTGATA TCCCGCACAT CTGGCTGTAT
    CTTGCCGAGC TCATAACACC TATCCTGCAA GAGGAAGGCA TCCCCATGGA GGAGCTGTTC
    AGGGAGATAA CAAAGCCCCT GGTGCCCCTT GGGAAGGCCA CCACGCTGCT GGTTGAGGTG
    CTGGGCTTAT TGTGCAAGGG CATGAGCCAG AAGACTGCGG GCAAGCTGTG GCGGGATGGG
    GGACTGAGCT GGAAGGAATT CCTGCCTGAG GACCAGGACA TCAACAAATT CGTCACAGAG
    CAGAAATTGG AGTACACGAT GGGGGACAAC TCGGACACGC CAAGCTGCAA GGAGCTGACC
    TCGGAGGAGC TGTGCAAGCA AATGGACAAA CTGCTGAAGG AGAACTCGAA CAACCAAAGA
    ATATATGACT GGATTGAGGC CAACCTAAGT GAGCAGCAGG TCTCGTCCAA CACATTTATC
    AGGGCCCTGA TGACATCCGT GTGCCATTCA GCCATCACCT TTGAGAACCC GTACCGTGTA
    GACGCCCTGG TCATTAGCAA CCGGGCCAAG CTGCTGCAGA AGTACATGCG GGATGAGCAG
    AAGGAGCTTC AGGCACTCTA CGCCTTGCAG GCTTTGGTGG TGAAGTTGGA CCAGCCTCCA
    AACCTGCTGC GGATGTTCTT TGATGCTCTC TACGATGAGG ACGTCATCAA GGAGGAGGCC
    TTCTACAAGT GGGAGTCCAG CAAGGACCCG GCCGAGCAGC AGGGCAAAGG GGTGGCCCTG
    AAATCAGTGA CAGCGTTTTT CACCTGGCTC CGGGAAGCAG AGGACGAGTC AGACAACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39995
    Clone ID Related Accession (Same CDS sequence) XM_015291540.1 , XM_015291540.2
    Accession Version XM_015291540.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGCTGGG TCGGATGGAG CCAGGCGGCC CCCCCGCAGC ATTTCTACCA GAACAGGGCC 
    CAGCCTCCCG CCAGTGCATC CCGCGTGCAG AGCAACACAA CGGCCCGGCC TGGCCCTCCT
    GCCCATGTGT ATCCAGCTGC TTCCCAGGTG ATGATGATAC CCTCTCAGAT ATCCTACACA
    CCTTCCCAAG GAGCCTATTA CATTCCCGGA CAGGGTCGTT CCACATACGT TGTCCCAACA
    CAGCAGTACC CGGTCCAGCC CGGTGCCCCT AGTTTTTACC CTGGAGCCAG CCCCACAGAG
    TTCGGGACTT ACGCGGGTGC CTATTACCCG GCCCAGGGGG TGCAGCAGTT CCCGGCGGGG
    GTCCCCACTG CCCAGGTCAT TGTGAGCCAG CAGCCACCGA TCCCCCCAAA ACGAGAGCGC
    AAGACGATCC GGATACGAGA CCCCAACCAA GGTGGCAAAG ACATCACTGA AGAAATTATG
    TCCGGAGCAA GGACCTCATC TACCCCCACC CCTCCCCAGG CTGGAAGCGG TTTGGAACCC
    CAGGCCAATG GAGAAACCCC TCATGTAGCA GTTATTGTTC GGCCAGATGA CCGCCCAAAG
    CCCGCGCTGG TGGTGAGCAA GCCCGTCTCC CTGGAGCCCA GCAAGTCAGC GTCCCCGTCG
    CCTCCCCCTC CCCTCATCCC CGAGGTGGAG CCTGTGGTGA TGTCAACTAT GACGCTGGTG
    CCAATGGAGC CCCCCATGGA AGCGGACACT AAAGTGGAGC TGGGCGAGGC GCCGCCCGAC
    CTGCACCAGA CGTTTAGCGC TATCACTACA GTGCCAGGGG CTGGGGAGCT GCCCCTTGTA
    CCCCCACCTG ACATGGACAC AGCGGCTGTG GTGGAGGAGG AGGTGGCAGA GGAGGTGGAA
    GAGGTTGCCA TTCCTCTCCT GGAGCCCACC TTGCAGGCGC CCGCCCTGCC CGAGGTGCCA
    TCGGTGCCTG CCGCCCCCCT CATGCCAGCC GTGCCCCCGG TGCCAGCTGC GCCGTCGCCG
    CCGCTCGTTG TCCCGCCGGT CCCTGAAGCA CCCGCCAAAC CCGCCTCTCC CAGCCCCCCG
    CCGCCCCAGG AAGAGCCCTG CCCTGAGCCT GTTGCTCAGC CTGCTGCTGA GGCCAATGGG
    GTCTTAGAGG AGGTGCCCCA GCCGCTCCCT GAGGCGCCCG TGTGCCAGCC GGTGCCTGTG
    CCCATATCGG AGCCTGCCCC GGTGCCCGCC CCAGCGCCCA CTCTGGACTC TCCCATTGCG
    CAGCCTGAGG AGCTGCCCTT GCCCAACGGG ATGGAGGGCT CCAGCAAAGT GGAGGCGAGC
    GAGGAGCAGC CTGAGTCGGA CATCAGCCCC ATCTCGGAGC CCGAGGAGCC AGTGCAGCCT
    GGAACCCCCG CCTCCCCCCC AGCAGAGGAG GAAGAGGAAG AGAGCGAAGG CCCTGCTGAG
    GCCCAGGAGC GGAGCTCAAG TCTGGCCCCT GCCCCGTCGC AGACCTTGGA GGCGACTGTG
    CAAGTTGCTG TGTCGGTGCC AAAGAAAAAA CGAAGGATGA AGGAGCTGAA CAAGAAGGAG
    GCAGTAGGCG ATTTGCTGGA TGCCTTTAAA GAGTCTCAGA TCAGCGACAG TGCCTCAGAG
    GTGGAAAACA AGCCTCCCCC TTCTGCCCCT GCCCGAGAAA CAGAGGACGC AGCCCCCACC
    CGTCCCCAGG AGGAGTCGGA GGAGACGTGG GAGGAGAAGG AGGACAAGCT GGCCCCAGAG
    AAGGGCAAGG CTGGGGACCA GAAGTACCGC TACAAGGAAG AGCAGTGGAA GCCATTGAAT
    CCTGAGGAGA AGAAGCGGTA TGACCGGGAG TTCCTGCTGG GCTTCCAGTT CATCTTTGCC
    AGCATGCAGA AACCTGAGGG GTTGCCCCAG ATCACGGATG TGGTGCTGGA CAAGCCCTGT
    GTACCTTCGC AGGCCAACAA GACCCCATTG CGGGCGCTCG ACCCCATCCG CCTCAGCGGC
    ATGAACTGCA GCCCTGACTT CACCCCCTCC TTTGCCAACC TTGGGCGGCC TGTCATGGGC
    AACCGGGGCC TGCCTTCAGG GTTGGGTCCT CGCCGCTCAC AGCAGACTCA GAGGAAGGAG
    CCCCGTAAAA TCATTGCCAC TGTGTCCCTC AATGAGGATG TCAAGTTGAA CAAGGCTGAG
    AAGGCCTGGA AACCCAGCAG CAAGCGTGCC TCTGAGGAGG AGGATCCGGA GAACATCAAG
    ACACAGGAAC TGCTTCGCCG CGTCCGCAGC ATCCTCAACA AGCTGACACC CCAGATGTTC
    CAGCAATTGA TGAAGCAGGT GATGGAGTTG TCCATCGACA CGGAGGAGAG ACTCAAGGGT
    GTCATTGACC TTGTCTTCGA GAAAGCCATC TCGGAGCCAA ACTTCTCTGT TGCCTACGCT
    AACATGTGCC GTTGCCTTAT GGGGCTCAAA GTGCCCACGA CAGACAAGCC CACAGTGACT
    GTGAACTTCC GCAAGCTGCT GCTCAACCGC TGCCAGAAGG AGTTTGAGAA GGACAAGGAT
    GACGACGAGA TCTTTGAGAA GCGGCAGAAA GAAATGGACG ATGCCAGTGC TCCCGAGGAG
    AAGGCGCGCA TGAAGGATGA GCTGGAGGAG GCGCGGGACA AGGCCCGACG GCGGTCCCTG
    GGTAATATCA AGTTCATTGG AGAGCTCTTC AAACTGAAGA TGCTGACAGA GGCCATCATG
    CATGACTGTG TGGTGAAGCT GCTCAAAAAC CACGATGAGG AGTCTCTTGA GTGCCTTTGC
    CGCCTGCTTA CAACCATTGG CAAGGACTTG GACTTTGAGA AGGCCAAGCC CAGAATGGAC
    CAGTACTTTA ACCAGATGGA GAAAATCATC AAGGAGAAGA AGACATCATC CCGAATCCGT
    TTTATGCTGC AGGATGTGAT TGATCTCAGG CGGAATAGCT GGGTGCCACG GCGAGGAGAT
    CAGGGCCCCA AAACCATCGA CCAGATCCAC AAAGAGGCGG AGATGGAGGA GCATCGGGAA
    CACATCAAAG TGCAGCAGCT CATGTCAAAA GACAAAAGGA GAGGACCTCC TGGACCATCC
    ATCAGCGGTG GACGCAGCAG CCTGGTTGCA GATGACGGCT GGAACACTGT GCCCATCAGC
    AAGGGCAACC GGCCTATTGA CACCAGCCGG CTAACGAAAA TCACCAAGCC TGGATTGATC
    GACTCCAACA ACCAGCTCTT TGCGCCAGGC GGGCGTCTGA GCTGGGGCAA AGGCAGTAGT
    GGAGGGTCTG GCGCAAAGCC TGCAGATTCA GCATCTGATT CAGGGCGACC GGCCACAAGC
    ACCTTGAACC GCTTCTCAGC ACTCCAGCAG TCCACCCCTG CTGAGAGCTC GGAGTCCCGT
    CGCGTGGTGC AGAGGAGCAG CTCCAGCCGC GACAGGTCAG AAAAGGCTGG GGACAGAGGG
    GACCGGGAGT CACGTTCAGA GAAGAGCAGT GACCGTCTGG AGCGTCCTGA TCGGGGAGAG
    CGGGGAGAGA GGAACAGGTC TGCTGTCACC AAGAGGAGCT TCAGCAAAGA GACAGAGGAC
    AGGAGCAGAG AACGGGAAAA GCAGAGTGGC CCTGAGGCCG TGCGTAAGAC TGCTAGCATG
    TCAGAGGAAC GGGACAGGAG CCGAGAGACG ATTAAACAAG AGCCAGCACC TCCTGCGGCA
    TCACCCAAAC CCATGCTGTC AGAGGAGGAG CTGGAGAAGA AATCTAAGGC GATCATAGAG
    GAATATCTGC ACATCAATGA TATGAAGGAG GCCCTGCAGT GTGTGCAGGA GCTGGGCAGC
    CCCTCCTTGC TCTACGTCTT CGTGCGGAAT GGCATTGAGT CCACGCTTGA GCGGAGCACG
    ATCTCCCGCG AGCACATGGG GGTCCTGCTG TGCCACCTGG TGAAGGCTGG CACGCTCTCC
    AAGGAGCAGT ACTATAAAGG GCTGCGGGAG ATCCTGGAGA TTGCAGAAGA CATGGAGATT
    GATATCCCGC ACATCTGGCT GTATCTTGCC GAGCTCATAA CACCTATCCT GCAAGAGGAA
    GGCATCCCCA TGGAGGAGCT GTTCAGGGAG ATAACAAAGC CCCTGGTGCC CCTTGGGAAG
    GCCACCACGC TGCTGGTTGA GGTGCTGGGC TTATTGTGCA AGGGCATGAG CCAGAAGACT
    GCGGGCAAGC TGTGGCGGGA TGGGGGACTG AGCTGGAAGG AATTCCTGCC TGAGGACCAG
    GACATCAACA AATTCGTCAC AGAGCAGAAA TTGGAGTACA CGATGGGGGA CAACTCGGAC
    ACGCCAAGCT GCAAGGAGCT GACCTCGGAG GAGCTGTGCA AGCAAATGGA CAAACTGCTG
    AAGGAGAACT CGAACAACCA AAGAATATAT GACTGGATTG AGGCCAACCT AAGTGAGCAG
    CAGGTCTCGT CCAACACATT TATCAGGGCC CTGATGACAT CCGTGTGCCA TTCAGCCATC
    ACCTTTGAGA ACCCGTACCG TGTAGACGCC CTGGTCATTA GCAACCGGGC CAAGCTGCTG
    CAGAAGTACA TGCGGGATGA GCAGAAGGAG CTTCAGGCAC TCTACGCCTT GCAGGCTTTG
    GTGGTGAAGT TGGACCAGCC TCCAAACCTG CTGCGGATGT TCTTTGATGC TCTCTACGAT
    GAGGACGTCA TCAAGGAGGA GGCCTTCTAC AAGTGGGAGT CCAGCAAGGA CCCGGCCGAG
    CAGCAGGGCA AAGGGGTGGC CCTGAAATCA GTGACAGCGT TTTTCACCTG GCTCCGGGAA
    GCAGAGGACG AGTCAGACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147026.1
    CDS88..4854
    Translation

    Target ORF information:

    RefSeq Version XM_015291540.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGCTGGG TCGGATGGAG CCAGGCGGCC CCCCCGCAGC ATTTCTACCA GAACAGGGCC 
    CAGCCTCCCG CCAGTGCATC CCGCGTGCAG AGCAACACAA CGGCCCGGCC TGGCCCTCCT
    GCCCATGTGT ATCCAGCTGC TTCCCAGGTG ATGATGATAC CCTCTCAGAT ATCCTACACA
    CCTTCCCAAG GAGCCTATTA CATTCCCGGA CAGGGTCGTT CCACATACGT TGTCCCAACA
    CAGCAGTACC CGGTCCAGCC CGGTGCCCCT AGTTTTTACC CTGGAGCCAG CCCCACAGAG
    TTCGGGACTT ACGCGGGTGC CTATTACCCG GCCCAGGGGG TGCAGCAGTT CCCGGCGGGG
    GTCCCCACTG CCCAGGTCAT TGTGAGCCAG CAGCCACCGA TCCCCCCAAA ACGAGAGCGC
    AAGACGATCC GGATACGAGA CCCCAACCAA GGTGGCAAAG ACATCACTGA AGAAATTATG
    TCCGGAGCAA GGACCTCATC TACCCCCACC CCTCCCCAGG CTGGAAGCGG TTTGGAACCC
    CAGGCCAATG GAGAAACCCC TCATGTAGCA GTTATTGTTC GGCCAGATGA CCGCCCAAAG
    CCCGCGCTGG TGGTGAGCAA GCCCGTCTCC CTGGAGCCCA GCAAGTCAGC GTCCCCGTCG
    CCTCCCCCTC CCCTCATCCC CGAGGTGGAG CCTGTGGTGA TGTCAACTAT GACGCTGGTG
    CCAATGGAGC CCCCCATGGA AGCGGACACT AAAGTGGAGC TGGGCGAGGC GCCGCCCGAC
    CTGCACCAGA CGTTTAGCGC TATCACTACA GTGCCAGGGG CTGGGGAGCT GCCCCTTGTA
    CCCCCACCTG ACATGGACAC AGCGGCTGTG GTGGAGGAGG AGGTGGCAGA GGAGGTGGAA
    GAGGTTGCCA TTCCTCTCCT GGAGCCCACC TTGCAGGCGC CCGCCCTGCC CGAGGTGCCA
    TCGGTGCCTG CCGCCCCCCT CATGCCAGCC GTGCCCCCGG TGCCAGCTGC GCCGTCGCCG
    CCGCTCGTTG TCCCGCCGGT CCCTGAAGCA CCCGCCAAAC CCGCCTCTCC CAGCCCCCCG
    CCGCCCCAGG AAGAGCCCTG CCCTGAGCCT GTTGCTCAGC CTGCTGCTGA GGCCAATGGG
    GTCTTAGAGG AGGTGCCCCA GCCGCTCCCT GAGGCGCCCG TGTGCCAGCC GGTGCCTGTG
    CCCATATCGG AGCCTGCCCC GGTGCCCGCC CCAGCGCCCA CTCTGGACTC TCCCATTGCG
    CAGCCTGAGG AGCTGCCCTT GCCCAACGGG ATGGAGGGCT CCAGCAAAGT GGAGGCGAGC
    GAGGAGCAGC CTGAGTCGGA CATCAGCCCC ATCTCGGAGC CCGAGGAGCC AGTGCAGCCT
    GGAACCCCCG CCTCCCCCCC AGCAGAGGAG GAAGAGGAAG AGAGCGAAGG CCCTGCTGAG
    GCCCAGGAGC GGAGCTCAAG TCTGGCCCCT GCCCCGTCGC AGACCTTGGA GGCGACTGTG
    CAAGTTGCTG TGTCGGTGCC AAAGAAAAAA CGAAGGATGA AGGAGCTGAA CAAGAAGGAG
    GCAGTAGGCG ATTTGCTGGA TGCCTTTAAA GAGTCTCAGA TCAGCGACAG TGCCTCAGAG
    GTGGAAAACA AGCCTCCCCC TTCTGCCCCT GCCCGAGAAA CAGAGGACGC AGCCCCCACC
    CGTCCCCAGG AGGAGTCGGA GGAGACGTGG GAGGAGAAGG AGGACAAGCT GGCCCCAGAG
    AAGGGCAAGG CTGGGGACCA GAAGTACCGC TACAAGGAAG AGCAGTGGAA GCCATTGAAT
    CCTGAGGAGA AGAAGCGGTA TGACCGGGAG TTCCTGCTGG GCTTCCAGTT CATCTTTGCC
    AGCATGCAGA AACCTGAGGG GTTGCCCCAG ATCACGGATG TGGTGCTGGA CAAGCCCTGT
    GTACCTTCGC AGGCCAACAA GACCCCATTG CGGGCGCTCG ACCCCATCCG CCTCAGCGGC
    ATGAACTGCA GCCCTGACTT CACCCCCTCC TTTGCCAACC TTGGGCGGCC TGTCATGGGC
    AACCGGGGCC TGCCTTCAGG GTTGGGTCCT CGCCGCTCAC AGCAGACTCA GAGGAAGGAG
    CCCCGTAAAA TCATTGCCAC TGTGTCCCTC AATGAGGATG TCAAGTTGAA CAAGGCTGAG
    AAGGCCTGGA AACCCAGCAG CAAGCGTGCC TCTGAGGAGG AGGATCCGGA GAACATCAAG
    ACACAGGAAC TGCTTCGCCG CGTCCGCAGC ATCCTCAACA AGCTGACACC CCAGATGTTC
    CAGCAATTGA TGAAGCAGGT GATGGAGTTG TCCATCGACA CGGAGGAGAG ACTCAAGGGT
    GTCATTGACC TTGTCTTCGA GAAAGCCATC TCGGAGCCAA ACTTCTCTGT TGCCTACGCT
    AACATGTGCC GTTGCCTTAT GGGGCTCAAA GTGCCCACGA CAGACAAGCC CACAGTGACT
    GTGAACTTCC GCAAGCTGCT GCTCAACCGC TGCCAGAAGG AGTTTGAGAA GGACAAGGAT
    GACGACGAGA TCTTTGAGAA GCGGCAGAAA GAAATGGACG ATGCCAGTGC TCCCGAGGAG
    AAGGCGCGCA TGAAGGATGA GCTGGAGGAG GCGCGGGACA AGGCCCGACG GCGGTCCCTG
    GGTAATATCA AGTTCATTGG AGAGCTCTTC AAACTGAAGA TGCTGACAGA GGCCATCATG
    CATGACTGTG TGGTGAAGCT GCTCAAAAAC CACGATGAGG AGTCTCTTGA GTGCCTTTGC
    CGCCTGCTTA CAACCATTGG CAAGGACTTG GACTTTGAGA AGGCCAAGCC CAGAATGGAC
    CAGTACTTTA ACCAGATGGA GAAAATCATC AAGGAGAAGA AGACATCATC CCGAATCCGT
    TTTATGCTGC AGGATGTGAT TGATCTCAGG CGGAATAGCT GGGTGCCACG GCGAGGAGAT
    CAGGGCCCCA AAACCATCGA CCAGATCCAC AAAGAGGCGG AGATGGAGGA GCATCGGGAA
    CACATCAAAG TGCAGCAGCT CATGTCAAAA GACAAAAGGA GAGGACCTCC TGGACCATCC
    ATCAGCGGTG GACGCAGCAG CCTGGTTGCA GATGACGGCT GGAACACTGT GCCCATCAGC
    AAGGGCAACC GGCCTATTGA CACCAGCCGG CTAACGAAAA TCACCAAGCC TGGATTGATC
    GACTCCAACA ACCAGCTCTT TGCGCCAGGC GGGCGTCTGA GCTGGGGCAA AGGCAGTAGT
    GGAGGGTCTG GCGCAAAGCC TGCAGATTCA GCATCTGATT CAGGGCGACC GGCCACAAGC
    ACCTTGAACC GCTTCTCAGC ACTCCAGCAG TCCACCCCTG CTGAGAGCTC GGAGTCCCGT
    CGCGTGGTGC AGAGGAGCAG CTCCAGCCGC GACAGGTCAG AAAAGGCTGG GGACAGAGGG
    GACCGGGAGT CACGTTCAGA GAAGAGCAGT GACCGTCTGG AGCGTCCTGA TCGGGGAGAG
    CGGGGAGAGA GGAACAGGTC TGCTGTCACC AAGAGGAGCT TCAGCAAAGA GACAGAGGAC
    AGGAGCAGAG AACGGGAAAA GCAGAGTGGC CCTGAGGCCG TGCGTAAGAC TGCTAGCATG
    TCAGAGGAAC GGGACAGGAG CCGAGAGACG ATTAAACAAG AGCCAGCACC TCCTGCGGCA
    TCACCCAAAC CCATGCTGTC AGAGGAGGAG CTGGAGAAGA AATCTAAGGC GATCATAGAG
    GAATATCTGC ACATCAATGA TATGAAGGAG GCCCTGCAGT GTGTGCAGGA GCTGGGCAGC
    CCCTCCTTGC TCTACGTCTT CGTGCGGAAT GGCATTGAGT CCACGCTTGA GCGGAGCACG
    ATCTCCCGCG AGCACATGGG GGTCCTGCTG TGCCACCTGG TGAAGGCTGG CACGCTCTCC
    AAGGAGCAGT ACTATAAAGG GCTGCGGGAG ATCCTGGAGA TTGCAGAAGA CATGGAGATT
    GATATCCCGC ACATCTGGCT GTATCTTGCC GAGCTCATAA CACCTATCCT GCAAGAGGAA
    GGCATCCCCA TGGAGGAGCT GTTCAGGGAG ATAACAAAGC CCCTGGTGCC CCTTGGGAAG
    GCCACCACGC TGCTGGTTGA GGTGCTGGGC TTATTGTGCA AGGGCATGAG CCAGAAGACT
    GCGGGCAAGC TGTGGCGGGA TGGGGGACTG AGCTGGAAGG AATTCCTGCC TGAGGACCAG
    GACATCAACA AATTCGTCAC AGAGCAGAAA TTGGAGTACA CGATGGGGGA CAACTCGGAC
    ACGCCAAGCT GCAAGGAGCT GACCTCGGAG GAGCTGTGCA AGCAAATGGA CAAACTGCTG
    AAGGAGAACT CGAACAACCA AAGAATATAT GACTGGATTG AGGCCAACCT AAGTGAGCAG
    CAGGTCTCGT CCAACACATT TATCAGGGCC CTGATGACAT CCGTGTGCCA TTCAGCCATC
    ACCTTTGAGA ACCCGTACCG TGTAGACGCC CTGGTCATTA GCAACCGGGC CAAGCTGCTG
    CAGAAGTACA TGCGGGATGA GCAGAAGGAG CTTCAGGCAC TCTACGCCTT GCAGGCTTTG
    GTGGTGAAGT TGGACCAGCC TCCAAACCTG CTGCGGATGT TCTTTGATGC TCTCTACGAT
    GAGGACGTCA TCAAGGAGGA GGCCTTCTAC AAGTGGGAGT CCAGCAAGGA CCCGGCCGAG
    CAGCAGGGCA AAGGGGTGGC CCTGAAATCA GTGACAGCGT TTTTCACCTG GCTCCGGGAA
    GCAGAGGACG AGTCAGACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39995
    Clone ID Related Accession (Same CDS sequence) XM_015291540.1 , XM_015291540.2
    Accession Version XM_015291540.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGCTGGG TCGGATGGAG CCAGGCGGCC CCCCCGCAGC ATTTCTACCA GAACAGGGCC 
    CAGCCTCCCG CCAGTGCATC CCGCGTGCAG AGCAACACAA CGGCCCGGCC TGGCCCTCCT
    GCCCATGTGT ATCCAGCTGC TTCCCAGGTG ATGATGATAC CCTCTCAGAT ATCCTACACA
    CCTTCCCAAG GAGCCTATTA CATTCCCGGA CAGGGTCGTT CCACATACGT TGTCCCAACA
    CAGCAGTACC CGGTCCAGCC CGGTGCCCCT AGTTTTTACC CTGGAGCCAG CCCCACAGAG
    TTCGGGACTT ACGCGGGTGC CTATTACCCG GCCCAGGGGG TGCAGCAGTT CCCGGCGGGG
    GTCCCCACTG CCCAGGTCAT TGTGAGCCAG CAGCCACCGA TCCCCCCAAA ACGAGAGCGC
    AAGACGATCC GGATACGAGA CCCCAACCAA GGTGGCAAAG ACATCACTGA AGAAATTATG
    TCCGGAGCAA GGACCTCATC TACCCCCACC CCTCCCCAGG CTGGAAGCGG TTTGGAACCC
    CAGGCCAATG GAGAAACCCC TCATGTAGCA GTTATTGTTC GGCCAGATGA CCGCCCAAAG
    CCCGCGCTGG TGGTGAGCAA GCCCGTCTCC CTGGAGCCCA GCAAGTCAGC GTCCCCGTCG
    CCTCCCCCTC CCCTCATCCC CGAGGTGGAG CCTGTGGTGA TGTCAACTAT GACGCTGGTG
    CCAATGGAGC CCCCCATGGA AGCGGACACT AAAGTGGAGC TGGGCGAGGC GCCGCCCGAC
    CTGCACCAGA CGTTTAGCGC TATCACTACA GTGCCAGGGG CTGGGGAGCT GCCCCTTGTA
    CCCCCACCTG ACATGGACAC AGCGGCTGTG GTGGAGGAGG AGGTGGCAGA GGAGGTGGAA
    GAGGTTGCCA TTCCTCTCCT GGAGCCCACC TTGCAGGCGC CCGCCCTGCC CGAGGTGCCA
    TCGGTGCCTG CCGCCCCCCT CATGCCAGCC GTGCCCCCGG TGCCAGCTGC GCCGTCGCCG
    CCGCTCGTTG TCCCGCCGGT CCCTGAAGCA CCCGCCAAAC CCGCCTCTCC CAGCCCCCCG
    CCGCCCCAGG AAGAGCCCTG CCCTGAGCCT GTTGCTCAGC CTGCTGCTGA GGCCAATGGG
    GTCTTAGAGG AGGTGCCCCA GCCGCTCCCT GAGGCGCCCG TGTGCCAGCC GGTGCCTGTG
    CCCATATCGG AGCCTGCCCC GGTGCCCGCC CCAGCGCCCA CTCTGGACTC TCCCATTGCG
    CAGCCTGAGG AGCTGCCCTT GCCCAACGGG ATGGAGGGCT CCAGCAAAGT GGAGGCGAGC
    GAGGAGCAGC CTGAGTCGGA CATCAGCCCC ATCTCGGAGC CCGAGGAGCC AGTGCAGCCT
    GGAACCCCCG CCTCCCCCCC AGCAGAGGAG GAAGAGGAAG AGAGCGAAGG CCCTGCTGAG
    GCCCAGGAGC GGAGCTCAAG TCTGGCCCCT GCCCCGTCGC AGACCTTGGA GGCGACTGTG
    CAAGTTGCTG TGTCGGTGCC AAAGAAAAAA CGAAGGATGA AGGAGCTGAA CAAGAAGGAG
    GCAGTAGGCG ATTTGCTGGA TGCCTTTAAA GAGTCTCAGA TCAGCGACAG TGCCTCAGAG
    GTGGAAAACA AGCCTCCCCC TTCTGCCCCT GCCCGAGAAA CAGAGGACGC AGCCCCCACC
    CGTCCCCAGG AGGAGTCGGA GGAGACGTGG GAGGAGAAGG AGGACAAGCT GGCCCCAGAG
    AAGGGCAAGG CTGGGGACCA GAAGTACCGC TACAAGGAAG AGCAGTGGAA GCCATTGAAT
    CCTGAGGAGA AGAAGCGGTA TGACCGGGAG TTCCTGCTGG GCTTCCAGTT CATCTTTGCC
    AGCATGCAGA AACCTGAGGG GTTGCCCCAG ATCACGGATG TGGTGCTGGA CAAGCCCTGT
    GTACCTTCGC AGGCCAACAA GACCCCATTG CGGGCGCTCG ACCCCATCCG CCTCAGCGGC
    ATGAACTGCA GCCCTGACTT CACCCCCTCC TTTGCCAACC TTGGGCGGCC TGTCATGGGC
    AACCGGGGCC TGCCTTCAGG GTTGGGTCCT CGCCGCTCAC AGCAGACTCA GAGGAAGGAG
    CCCCGTAAAA TCATTGCCAC TGTGTCCCTC AATGAGGATG TCAAGTTGAA CAAGGCTGAG
    AAGGCCTGGA AACCCAGCAG CAAGCGTGCC TCTGAGGAGG AGGATCCGGA GAACATCAAG
    ACACAGGAAC TGCTTCGCCG CGTCCGCAGC ATCCTCAACA AGCTGACACC CCAGATGTTC
    CAGCAATTGA TGAAGCAGGT GATGGAGTTG TCCATCGACA CGGAGGAGAG ACTCAAGGGT
    GTCATTGACC TTGTCTTCGA GAAAGCCATC TCGGAGCCAA ACTTCTCTGT TGCCTACGCT
    AACATGTGCC GTTGCCTTAT GGGGCTCAAA GTGCCCACGA CAGACAAGCC CACAGTGACT
    GTGAACTTCC GCAAGCTGCT GCTCAACCGC TGCCAGAAGG AGTTTGAGAA GGACAAGGAT
    GACGACGAGA TCTTTGAGAA GCGGCAGAAA GAAATGGACG ATGCCAGTGC TCCCGAGGAG
    AAGGCGCGCA TGAAGGATGA GCTGGAGGAG GCGCGGGACA AGGCCCGACG GCGGTCCCTG
    GGTAATATCA AGTTCATTGG AGAGCTCTTC AAACTGAAGA TGCTGACAGA GGCCATCATG
    CATGACTGTG TGGTGAAGCT GCTCAAAAAC CACGATGAGG AGTCTCTTGA GTGCCTTTGC
    CGCCTGCTTA CAACCATTGG CAAGGACTTG GACTTTGAGA AGGCCAAGCC CAGAATGGAC
    CAGTACTTTA ACCAGATGGA GAAAATCATC AAGGAGAAGA AGACATCATC CCGAATCCGT
    TTTATGCTGC AGGATGTGAT TGATCTCAGG CGGAATAGCT GGGTGCCACG GCGAGGAGAT
    CAGGGCCCCA AAACCATCGA CCAGATCCAC AAAGAGGCGG AGATGGAGGA GCATCGGGAA
    CACATCAAAG TGCAGCAGCT CATGTCAAAA GACAAAAGGA GAGGACCTCC TGGACCATCC
    ATCAGCGGTG GACGCAGCAG CCTGGTTGCA GATGACGGCT GGAACACTGT GCCCATCAGC
    AAGGGCAACC GGCCTATTGA CACCAGCCGG CTAACGAAAA TCACCAAGCC TGGATTGATC
    GACTCCAACA ACCAGCTCTT TGCGCCAGGC GGGCGTCTGA GCTGGGGCAA AGGCAGTAGT
    GGAGGGTCTG GCGCAAAGCC TGCAGATTCA GCATCTGATT CAGGGCGACC GGCCACAAGC
    ACCTTGAACC GCTTCTCAGC ACTCCAGCAG TCCACCCCTG CTGAGAGCTC GGAGTCCCGT
    CGCGTGGTGC AGAGGAGCAG CTCCAGCCGC GACAGGTCAG AAAAGGCTGG GGACAGAGGG
    GACCGGGAGT CACGTTCAGA GAAGAGCAGT GACCGTCTGG AGCGTCCTGA TCGGGGAGAG
    CGGGGAGAGA GGAACAGGTC TGCTGTCACC AAGAGGAGCT TCAGCAAAGA GACAGAGGAC
    AGGAGCAGAG AACGGGAAAA GCAGAGTGGC CCTGAGGCCG TGCGTAAGAC TGCTAGCATG
    TCAGAGGAAC GGGACAGGAG CCGAGAGACG ATTAAACAAG AGCCAGCACC TCCTGCGGCA
    TCACCCAAAC CCATGCTGTC AGAGGAGGAG CTGGAGAAGA AATCTAAGGC GATCATAGAG
    GAATATCTGC ACATCAATGA TATGAAGGAG GCCCTGCAGT GTGTGCAGGA GCTGGGCAGC
    CCCTCCTTGC TCTACGTCTT CGTGCGGAAT GGCATTGAGT CCACGCTTGA GCGGAGCACG
    ATCTCCCGCG AGCACATGGG GGTCCTGCTG TGCCACCTGG TGAAGGCTGG CACGCTCTCC
    AAGGAGCAGT ACTATAAAGG GCTGCGGGAG ATCCTGGAGA TTGCAGAAGA CATGGAGATT
    GATATCCCGC ACATCTGGCT GTATCTTGCC GAGCTCATAA CACCTATCCT GCAAGAGGAA
    GGCATCCCCA TGGAGGAGCT GTTCAGGGAG ATAACAAAGC CCCTGGTGCC CCTTGGGAAG
    GCCACCACGC TGCTGGTTGA GGTGCTGGGC TTATTGTGCA AGGGCATGAG CCAGAAGACT
    GCGGGCAAGC TGTGGCGGGA TGGGGGACTG AGCTGGAAGG AATTCCTGCC TGAGGACCAG
    GACATCAACA AATTCGTCAC AGAGCAGAAA TTGGAGTACA CGATGGGGGA CAACTCGGAC
    ACGCCAAGCT GCAAGGAGCT GACCTCGGAG GAGCTGTGCA AGCAAATGGA CAAACTGCTG
    AAGGAGAACT CGAACAACCA AAGAATATAT GACTGGATTG AGGCCAACCT AAGTGAGCAG
    CAGGTCTCGT CCAACACATT TATCAGGGCC CTGATGACAT CCGTGTGCCA TTCAGCCATC
    ACCTTTGAGA ACCCGTACCG TGTAGACGCC CTGGTCATTA GCAACCGGGC CAAGCTGCTG
    CAGAAGTACA TGCGGGATGA GCAGAAGGAG CTTCAGGCAC TCTACGCCTT GCAGGCTTTG
    GTGGTGAAGT TGGACCAGCC TCCAAACCTG CTGCGGATGT TCTTTGATGC TCTCTACGAT
    GAGGACGTCA TCAAGGAGGA GGCCTTCTAC AAGTGGGAGT CCAGCAAGGA CCCGGCCGAG
    CAGCAGGGCA AAGGGGTGGC CCTGAAATCA GTGACAGCGT TTTTCACCTG GCTCCGGGAA
    GCAGAGGACG AGTCAGACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147026.1
    CDS88..4854
    Translation

    Target ORF information:

    RefSeq Version XM_015291540.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGCTGGG TCGGATGGAG CCAGGCGGCC CCCCCGCAGC ATTTCTACCA GAACAGGGCC 
    CAGCCTCCCG CCAGTGCATC CCGCGTGCAG AGCAACACAA CGGCCCGGCC TGGCCCTCCT
    GCCCATGTGT ATCCAGCTGC TTCCCAGGTG ATGATGATAC CCTCTCAGAT ATCCTACACA
    CCTTCCCAAG GAGCCTATTA CATTCCCGGA CAGGGTCGTT CCACATACGT TGTCCCAACA
    CAGCAGTACC CGGTCCAGCC CGGTGCCCCT AGTTTTTACC CTGGAGCCAG CCCCACAGAG
    TTCGGGACTT ACGCGGGTGC CTATTACCCG GCCCAGGGGG TGCAGCAGTT CCCGGCGGGG
    GTCCCCACTG CCCAGGTCAT TGTGAGCCAG CAGCCACCGA TCCCCCCAAA ACGAGAGCGC
    AAGACGATCC GGATACGAGA CCCCAACCAA GGTGGCAAAG ACATCACTGA AGAAATTATG
    TCCGGAGCAA GGACCTCATC TACCCCCACC CCTCCCCAGG CTGGAAGCGG TTTGGAACCC
    CAGGCCAATG GAGAAACCCC TCATGTAGCA GTTATTGTTC GGCCAGATGA CCGCCCAAAG
    CCCGCGCTGG TGGTGAGCAA GCCCGTCTCC CTGGAGCCCA GCAAGTCAGC GTCCCCGTCG
    CCTCCCCCTC CCCTCATCCC CGAGGTGGAG CCTGTGGTGA TGTCAACTAT GACGCTGGTG
    CCAATGGAGC CCCCCATGGA AGCGGACACT AAAGTGGAGC TGGGCGAGGC GCCGCCCGAC
    CTGCACCAGA CGTTTAGCGC TATCACTACA GTGCCAGGGG CTGGGGAGCT GCCCCTTGTA
    CCCCCACCTG ACATGGACAC AGCGGCTGTG GTGGAGGAGG AGGTGGCAGA GGAGGTGGAA
    GAGGTTGCCA TTCCTCTCCT GGAGCCCACC TTGCAGGCGC CCGCCCTGCC CGAGGTGCCA
    TCGGTGCCTG CCGCCCCCCT CATGCCAGCC GTGCCCCCGG TGCCAGCTGC GCCGTCGCCG
    CCGCTCGTTG TCCCGCCGGT CCCTGAAGCA CCCGCCAAAC CCGCCTCTCC CAGCCCCCCG
    CCGCCCCAGG AAGAGCCCTG CCCTGAGCCT GTTGCTCAGC CTGCTGCTGA GGCCAATGGG
    GTCTTAGAGG AGGTGCCCCA GCCGCTCCCT GAGGCGCCCG TGTGCCAGCC GGTGCCTGTG
    CCCATATCGG AGCCTGCCCC GGTGCCCGCC CCAGCGCCCA CTCTGGACTC TCCCATTGCG
    CAGCCTGAGG AGCTGCCCTT GCCCAACGGG ATGGAGGGCT CCAGCAAAGT GGAGGCGAGC
    GAGGAGCAGC CTGAGTCGGA CATCAGCCCC ATCTCGGAGC CCGAGGAGCC AGTGCAGCCT
    GGAACCCCCG CCTCCCCCCC AGCAGAGGAG GAAGAGGAAG AGAGCGAAGG CCCTGCTGAG
    GCCCAGGAGC GGAGCTCAAG TCTGGCCCCT GCCCCGTCGC AGACCTTGGA GGCGACTGTG
    CAAGTTGCTG TGTCGGTGCC AAAGAAAAAA CGAAGGATGA AGGAGCTGAA CAAGAAGGAG
    GCAGTAGGCG ATTTGCTGGA TGCCTTTAAA GAGTCTCAGA TCAGCGACAG TGCCTCAGAG
    GTGGAAAACA AGCCTCCCCC TTCTGCCCCT GCCCGAGAAA CAGAGGACGC AGCCCCCACC
    CGTCCCCAGG AGGAGTCGGA GGAGACGTGG GAGGAGAAGG AGGACAAGCT GGCCCCAGAG
    AAGGGCAAGG CTGGGGACCA GAAGTACCGC TACAAGGAAG AGCAGTGGAA GCCATTGAAT
    CCTGAGGAGA AGAAGCGGTA TGACCGGGAG TTCCTGCTGG GCTTCCAGTT CATCTTTGCC
    AGCATGCAGA AACCTGAGGG GTTGCCCCAG ATCACGGATG TGGTGCTGGA CAAGCCCTGT
    GTACCTTCGC AGGCCAACAA GACCCCATTG CGGGCGCTCG ACCCCATCCG CCTCAGCGGC
    ATGAACTGCA GCCCTGACTT CACCCCCTCC TTTGCCAACC TTGGGCGGCC TGTCATGGGC
    AACCGGGGCC TGCCTTCAGG GTTGGGTCCT CGCCGCTCAC AGCAGACTCA GAGGAAGGAG
    CCCCGTAAAA TCATTGCCAC TGTGTCCCTC AATGAGGATG TCAAGTTGAA CAAGGCTGAG
    AAGGCCTGGA AACCCAGCAG CAAGCGTGCC TCTGAGGAGG AGGATCCGGA GAACATCAAG
    ACACAGGAAC TGCTTCGCCG CGTCCGCAGC ATCCTCAACA AGCTGACACC CCAGATGTTC
    CAGCAATTGA TGAAGCAGGT GATGGAGTTG TCCATCGACA CGGAGGAGAG ACTCAAGGGT
    GTCATTGACC TTGTCTTCGA GAAAGCCATC TCGGAGCCAA ACTTCTCTGT TGCCTACGCT
    AACATGTGCC GTTGCCTTAT GGGGCTCAAA GTGCCCACGA CAGACAAGCC CACAGTGACT
    GTGAACTTCC GCAAGCTGCT GCTCAACCGC TGCCAGAAGG AGTTTGAGAA GGACAAGGAT
    GACGACGAGA TCTTTGAGAA GCGGCAGAAA GAAATGGACG ATGCCAGTGC TCCCGAGGAG
    AAGGCGCGCA TGAAGGATGA GCTGGAGGAG GCGCGGGACA AGGCCCGACG GCGGTCCCTG
    GGTAATATCA AGTTCATTGG AGAGCTCTTC AAACTGAAGA TGCTGACAGA GGCCATCATG
    CATGACTGTG TGGTGAAGCT GCTCAAAAAC CACGATGAGG AGTCTCTTGA GTGCCTTTGC
    CGCCTGCTTA CAACCATTGG CAAGGACTTG GACTTTGAGA AGGCCAAGCC CAGAATGGAC
    CAGTACTTTA ACCAGATGGA GAAAATCATC AAGGAGAAGA AGACATCATC CCGAATCCGT
    TTTATGCTGC AGGATGTGAT TGATCTCAGG CGGAATAGCT GGGTGCCACG GCGAGGAGAT
    CAGGGCCCCA AAACCATCGA CCAGATCCAC AAAGAGGCGG AGATGGAGGA GCATCGGGAA
    CACATCAAAG TGCAGCAGCT CATGTCAAAA GACAAAAGGA GAGGACCTCC TGGACCATCC
    ATCAGCGGTG GACGCAGCAG CCTGGTTGCA GATGACGGCT GGAACACTGT GCCCATCAGC
    AAGGGCAACC GGCCTATTGA CACCAGCCGG CTAACGAAAA TCACCAAGCC TGGATTGATC
    GACTCCAACA ACCAGCTCTT TGCGCCAGGC GGGCGTCTGA GCTGGGGCAA AGGCAGTAGT
    GGAGGGTCTG GCGCAAAGCC TGCAGATTCA GCATCTGATT CAGGGCGACC GGCCACAAGC
    ACCTTGAACC GCTTCTCAGC ACTCCAGCAG TCCACCCCTG CTGAGAGCTC GGAGTCCCGT
    CGCGTGGTGC AGAGGAGCAG CTCCAGCCGC GACAGGTCAG AAAAGGCTGG GGACAGAGGG
    GACCGGGAGT CACGTTCAGA GAAGAGCAGT GACCGTCTGG AGCGTCCTGA TCGGGGAGAG
    CGGGGAGAGA GGAACAGGTC TGCTGTCACC AAGAGGAGCT TCAGCAAAGA GACAGAGGAC
    AGGAGCAGAG AACGGGAAAA GCAGAGTGGC CCTGAGGCCG TGCGTAAGAC TGCTAGCATG
    TCAGAGGAAC GGGACAGGAG CCGAGAGACG ATTAAACAAG AGCCAGCACC TCCTGCGGCA
    TCACCCAAAC CCATGCTGTC AGAGGAGGAG CTGGAGAAGA AATCTAAGGC GATCATAGAG
    GAATATCTGC ACATCAATGA TATGAAGGAG GCCCTGCAGT GTGTGCAGGA GCTGGGCAGC
    CCCTCCTTGC TCTACGTCTT CGTGCGGAAT GGCATTGAGT CCACGCTTGA GCGGAGCACG
    ATCTCCCGCG AGCACATGGG GGTCCTGCTG TGCCACCTGG TGAAGGCTGG CACGCTCTCC
    AAGGAGCAGT ACTATAAAGG GCTGCGGGAG ATCCTGGAGA TTGCAGAAGA CATGGAGATT
    GATATCCCGC ACATCTGGCT GTATCTTGCC GAGCTCATAA CACCTATCCT GCAAGAGGAA
    GGCATCCCCA TGGAGGAGCT GTTCAGGGAG ATAACAAAGC CCCTGGTGCC CCTTGGGAAG
    GCCACCACGC TGCTGGTTGA GGTGCTGGGC TTATTGTGCA AGGGCATGAG CCAGAAGACT
    GCGGGCAAGC TGTGGCGGGA TGGGGGACTG AGCTGGAAGG AATTCCTGCC TGAGGACCAG
    GACATCAACA AATTCGTCAC AGAGCAGAAA TTGGAGTACA CGATGGGGGA CAACTCGGAC
    ACGCCAAGCT GCAAGGAGCT GACCTCGGAG GAGCTGTGCA AGCAAATGGA CAAACTGCTG
    AAGGAGAACT CGAACAACCA AAGAATATAT GACTGGATTG AGGCCAACCT AAGTGAGCAG
    CAGGTCTCGT CCAACACATT TATCAGGGCC CTGATGACAT CCGTGTGCCA TTCAGCCATC
    ACCTTTGAGA ACCCGTACCG TGTAGACGCC CTGGTCATTA GCAACCGGGC CAAGCTGCTG
    CAGAAGTACA TGCGGGATGA GCAGAAGGAG CTTCAGGCAC TCTACGCCTT GCAGGCTTTG
    GTGGTGAAGT TGGACCAGCC TCCAAACCTG CTGCGGATGT TCTTTGATGC TCTCTACGAT
    GAGGACGTCA TCAAGGAGGA GGCCTTCTAC AAGTGGGAGT CCAGCAAGGA CCCGGCCGAG
    CAGCAGGGCA AAGGGGTGGC CCTGAAATCA GTGACAGCGT TTTTCACCTG GCTCCGGGAA
    GCAGAGGACG AGTCAGACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39997
    Clone ID Related Accession (Same CDS sequence) XM_015291543.2 , XM_015291543.1
    Accession Version XM_015291543.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGGCC
    AACAAGACCC CATTGCGGGC GCTCGACCCC ATCCGCCTCA GCGGCATGAA CTGCAGCCCT
    GACTTCACCC CCTCCTTTGC CAACCTTGGG CGGCCTGTCA TGGGCAACCG GGGCCTGCCT
    TCAGGGTTGG GTCCTCGCCG CTCACAGCAG ACTCAGAGGA AGGAGCCCCG TAAAATCATT
    GCCACTGTGT CCCTCAATGA GGATGTCAAG TTGAACAAGG CTGAGAAGGC CTGGAAACCC
    AGCAGCAAGC GTGCCTCTGA GGAGGAGGAT CCGGAGAACA TCAAGACACA GGAACTGCTT
    CGCCGCGTCC GCAGCATCCT CAACAAGCTG ACACCCCAGA TGTTCCAGCA ATTGATGAAG
    CAGGTGATGG AGTTGTCCAT CGACACGGAG GAGAGACTCA AGGGTGTCAT TGACCTTGTC
    TTCGAGAAAG CCATCTCGGA GCCAAACTTC TCTGTTGCCT ACGCTAACAT GTGCCGTTGC
    CTTATGGGGC TCAAAGTGCC CACGACAGAC AAGCCCACAG TGACTGTGAA CTTCCGCAAG
    CTGCTGCTCA ACCGCTGCCA GAAGGAGTTT GAGAAGGACA AGGATGACGA CGAGATCTTT
    GAGAAGCGGC AGAAAGAAAT GGACGATGCC AGTGCTCCCG AGGAGAAGGC GCGCATGAAG
    GATGAGCTGG AGGAGGCGCG GGACAAGGCC CGACGGCGGT CCCTGGGTAA TATCAAGTTC
    ATTGGAGAGC TCTTCAAACT GAAGATGCTG ACAGAGGCCA TCATGCATGA CTGTGTGGTG
    AAGCTGCTCA AAAACCACGA TGAGGAGTCT CTTGAGTGCC TTTGCCGCCT GCTTACAACC
    ATTGGCAAGG ACTTGGACTT TGAGAAGGCC AAGCCCAGAA TGGACCAGTA CTTTAACCAG
    ATGGAGAAAA TCATCAAGGA GAAGAAGACA TCATCCCGAA TCCGTTTTAT GCTGCAGGAT
    GTGATTGATC TCAGGCGGAA TAGCTGGGTG CCACGGCGAG GAGATCAGGG CCCCAAAACC
    ATCGACCAGA TCCACAAAGA GGCGGAGATG GAGGAGCATC GGGAACACAT CAAAGTGCAG
    CAGCTCATGT CAAAAGACAA AAGGAGAGGA CCTCCTGGAC CATCCATCAG CGGTGGACGC
    AGCAGCCTGG TTGCAGATGA CGGCTGGAAC ACTGTGCCCA TCAGCAAGGG CAACCGGCCT
    ATTGACACCA GCCGGCTAAC GAAAATCACC AAGCCTGGAT TGATCGACTC CAACAACCAG
    CTCTTTGCGC CAGGCGGGCG TCTGAGCTGG GGCAAAGGCA GTAGTGGAGG GTCTGGCGCA
    AAGCCTGCAG ATTCAGCATC TGATTCAGGG CGACCGGCCA CAAGCACCTT GAACCGCTTC
    TCAGCACTCC AGCAGTCCAC CCCTGCTGAG AGCTCGGAGT CCCGTCGCGT GGTGCAGAGG
    AGCAGCTCCA GCCGCGACAG GTCAGAAAAG GCTGGGGACA GAGGGGACCG GGAGTCACGT
    TCAGAGAAGA GCAGTGACCG TCTGGAGCGT CCTGATCGGG GAGAGCGGGG AGAGAGGAAC
    AGGTCTGCTG TCACCAAGAG GAGCTTCAGC AAAGAGACAG AGGACAGGAG CAGAGAACGG
    GAAAAGCAGA GTGGCCCTGA GGCCGTGCGT AAGACTGCTA GCATGTCAGA GGAACGGGAC
    AGGAGCCGAG AGACGATTAA ACAAGAGCCA GCACCTCCTG CGGCATCACC CAAACCCATG
    CTGTCAGAGG AGGAGCTGGA GAAGAAATCT AAGGCGATCA TAGAGGAATA TCTGCACATC
    AATGATATGA AGGAGGCCCT GCAGTGTGTG CAGGAGCTGG GCAGCCCCTC CTTGCTCTAC
    GTCTTCGTGC GGAATGGCAT TGAGTCCACG CTTGAGCGGA GCACGATCTC CCGCGAGCAC
    ATGGGGGTCC TGCTGTGCCA CCTGGTGAAG GCTGGCACGC TCTCCAAGGA GCAGTACTAT
    AAAGGGCTGC GGGAGATCCT GGAGATTGCA GAAGACATGG AGATTGATAT CCCGCACATC
    TGGCTGTATC TTGCCGAGCT CATAACACCT ATCCTGCAAG AGGAAGGCAT CCCCATGGAG
    GAGCTGTTCA GGGAGATAAC AAAGCCCCTG GTGCCCCTTG GGAAGGCCAC CACGCTGCTG
    GTTGAGGTGC TGGGCTTATT GTGCAAGGGC ATGAGCCAGA AGACTGCGGG CAAGCTGTGG
    CGGGATGGGG GACTGAGCTG GAAGGAATTC CTGCCTGAGG ACCAGGACAT CAACAAATTC
    GTCACAGAGC AGAAATTGGA GTACACGATG GGGGACAACT CGGACACGCC AAGCTGCAAG
    GAGCTGACCT CGGAGGAGCT GTGCAAGCAA ATGGACAAAC TGCTGAAGGA GAACTCGAAC
    AACCAAAGAA TATATGACTG GATTGAGGCC AACCTAAGTG AGCAGCAGGT CTCGTCCAAC
    ACATTTATCA GGGCCCTGAT GACATCCGTG TGCCATTCAG CCATCACCTT TGAGAACCCG
    TACCGTGTAG ACGCCCTGGT CATTAGCAAC CGGGCCAAGC TGCTGCAGAA GTACATGCGG
    GATGAGCAGA AGGAGCTTCA GGCACTCTAC GCCTTGCAGG CTTTGGTGGT GAAGTTGGAC
    CAGCCTCCAA ACCTGCTGCG GATGTTCTTT GATGCTCTCT ACGATGAGGA CGTCATCAAG
    GAGGAGGCCT TCTACAAGTG GGAGTCCAGC AAGGACCCGG CCGAGCAGCA GGGCAAAGGG
    GTGGCCCTGA AATCAGTGAC AGCGTTTTTC ACCTGGCTCC GGGAAGCAGA GGACGAGTCA
    GACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147029.1
    CDS74..4945
    Translation

    Target ORF information:

    RefSeq Version XM_015291543.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291543.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACAAAG CTCCACAGCC CACAGGAGGA GCCCCGACAG CCCCGCACCC TGCCCCTTCT 
    CCTGGACTTC CACAGTCAGC GTTCCCACCT GGTCAGACGG CACCTGTGAT TTTTAACCCG
    ACACCGACCT CACAAATGAA TACGCCTTCT CAGCCGCGAC AGCATTTCTA CCAGAACAGG
    GCCCAGCCTC CCGCCAGTGC ATCCCGCGTG CAGAGCAACA CAACGGCCCG GCCTGGCCCT
    CCTGCCCATG TGTATCCAGC TGCTTCCCAG GTGATGATGA TACCCTCTCA GATATCCTAC
    ACACCTTCCC AAGGAGCCTA TTACATTCCC GGACAGGGTC GTTCCACATA CGTTGTCCCA
    ACACAGCAGT ACCCGGTCCA GCCCGGTGCC CCTAGTTTTT ACCCTGGAGC CAGCCCCACA
    GAGTTCGGGA CTTACGCGGG TGCCTATTAC CCGGCCCAGG GGGTGCAGCA GTTCCCGGCG
    GGGGTCCCCA CTGCCCAGGT CATTGTGAGC CAGCAGCCAC CGATCCCCCC AAAACGAGAG
    CGCAAGACGA TCCGGATACG AGACCCCAAC CAAGGTGGCA AAGACATCAC TGAAGAAATT
    ATGTCCGGAG CAAGGACCTC ATCTACCCCC ACCCCTCCCC AGGCTGGAAG CGGTTTGGAA
    CCCCAGGCCA ATGGAGAAAC CCCTCATGTA GCAGTTATTG TTCGGCCAGA TGACCGCCCA
    AAGCCCGCGC TGGTGGTGAG CAAGCCCGTC TCCCTGGAGC CCAGCAAGTC AGCGTCCCCG
    TCGCCTCCCC CTCCCCTCAT CCCCGAGGTG GAGCCTGTGG TGATGTCAAC TATGACGCTG
    GTGCCAATGG AGCCCCCCAT GGAAGCGGAC ACTAAAGTGG AGCTGGGCGA GGCGCCGCCC
    GACCTGCACC AGACGTTTAG CGCTATCACT ACAGTGCCAG GGGCTGGGGA GCTGCCCCTT
    GTACCCCCAC CTGACATGGA CACAGCGGCT GTGGTGGAGG AGGAGGTGGC AGAGGAGGTG
    GAAGAGGTTG CCATTCCTCT CCTGGAGCCC ACCTTGCAGG CGCCCGCCCT GCCCGAGGTG
    CCATCGGTGC CTGCCGCCCC CCTCATGCCA GCCGTGCCCC CGGTGCCAGC TGCGCCGTCG
    CCGCCGCTCG TTGTCCCGCC GGTCCCTGAA GCACCCGCCA AACCCGCCTC TCCCAGCCCC
    CCGCCGCCCC AGGAAGAGCC CTGCCCTGAG CCTGTTGCTC AGCCTGCTGC TGAGGCCAAT
    GGGGTCTTAG AGGAGGTGCC CCAGCCGCTC CCTGAGGCGC CCGTGTGCCA GCCGGTGCCT
    GTGCCCATAT CGGAGCCTGC CCCGGTGCCC GCCCCAGCGC CCACTCTGGA CTCTCCCATT
    GCGCAGCCTG AGGAGCTGCC CTTGCCCAAC GGGATGGAGG GCTCCAGCAA AGTGGAGGCG
    AGCGAGGAGC AGCCTGAGTC GGACATCAGC CCCATCTCGG AGCCCGAGGA GCCAGTGCAG
    CCTGGAACCC CCGCCTCCCC CCCAGCAGAG GAGGAAGAGG AAGAGAGCGA AGGCCCTGCT
    GAGGCCCAGG AGCGGAGCTC AAGTCTGGCC CCTGCCCCGT CGCAGACCTT GGAGGCGACT
    GTGCAAGTTG CTGTGTCGGT GCCAAAGAAA AAACGAAGGA TGAAGGAGCT GAACAAGAAG
    GAGGCAGTAG GCGATTTGCT GGATGCCTTT AAAGAGTCTC AGATCAGCGA CAGTGCCTCA
    GAGGTGGAAA ACAAGCCTCC CCCTTCTGCC CCTGCCCGAG AAACAGAGGA CGCAGCCCCC
    ACCCGTCCCC AGGAGGAGTC GGAGGAGACG TGGGAGGAGA AGGAGGACAA GCTGGCCCCA
    GAGAAGGGCA AGGCTGGGGA CCAGAAGTAC CGCTACAAGG AAGAGCAGTG GAAGCCATTG
    AATCCTGAGG AGAAGAAGCG GTATGACCGG GAGTTCCTGC TGGGCTTCCA GTTCATCTTT
    GCCAGCATGC AGAAACCTGA GGGGTTGCCC CAGATCACGG ATGTGGTGCT GGACAAGGCC
    AACAAGACCC CATTGCGGGC GCTCGACCCC ATCCGCCTCA GCGGCATGAA CTGCAGCCCT
    GACTTCACCC CCTCCTTTGC CAACCTTGGG CGGCCTGTCA TGGGCAACCG GGGCCTGCCT
    TCAGGGTTGG GTCCTCGCCG CTCACAGCAG ACTCAGAGGA AGGAGCCCCG TAAAATCATT
    GCCACTGTGT CCCTCAATGA GGATGTCAAG TTGAACAAGG CTGAGAAGGC CTGGAAACCC
    AGCAGCAAGC GTGCCTCTGA GGAGGAGGAT CCGGAGAACA TCAAGACACA GGAACTGCTT
    CGCCGCGTCC GCAGCATCCT CAACAAGCTG ACACCCCAGA TGTTCCAGCA ATTGATGAAG
    CAGGTGATGG AGTTGTCCAT CGACACGGAG GAGAGACTCA AGGGTGTCAT TGACCTTGTC
    TTCGAGAAAG CCATCTCGGA GCCAAACTTC TCTGTTGCCT ACGCTAACAT GTGCCGTTGC
    CTTATGGGGC TCAAAGTGCC CACGACAGAC AAGCCCACAG TGACTGTGAA CTTCCGCAAG
    CTGCTGCTCA ACCGCTGCCA GAAGGAGTTT GAGAAGGACA AGGATGACGA CGAGATCTTT
    GAGAAGCGGC AGAAAGAAAT GGACGATGCC AGTGCTCCCG AGGAGAAGGC GCGCATGAAG
    GATGAGCTGG AGGAGGCGCG GGACAAGGCC CGACGGCGGT CCCTGGGTAA TATCAAGTTC
    ATTGGAGAGC TCTTCAAACT GAAGATGCTG ACAGAGGCCA TCATGCATGA CTGTGTGGTG
    AAGCTGCTCA AAAACCACGA TGAGGAGTCT CTTGAGTGCC TTTGCCGCCT GCTTACAACC
    ATTGGCAAGG ACTTGGACTT TGAGAAGGCC AAGCCCAGAA TGGACCAGTA CTTTAACCAG
    ATGGAGAAAA TCATCAAGGA GAAGAAGACA TCATCCCGAA TCCGTTTTAT GCTGCAGGAT
    GTGATTGATC TCAGGCGGAA TAGCTGGGTG CCACGGCGAG GAGATCAGGG CCCCAAAACC
    ATCGACCAGA TCCACAAAGA GGCGGAGATG GAGGAGCATC GGGAACACAT CAAAGTGCAG
    CAGCTCATGT CAAAAGACAA AAGGAGAGGA CCTCCTGGAC CATCCATCAG CGGTGGACGC
    AGCAGCCTGG TTGCAGATGA CGGCTGGAAC ACTGTGCCCA TCAGCAAGGG CAACCGGCCT
    ATTGACACCA GCCGGCTAAC GAAAATCACC AAGCCTGGAT TGATCGACTC CAACAACCAG
    CTCTTTGCGC CAGGCGGGCG TCTGAGCTGG GGCAAAGGCA GTAGTGGAGG GTCTGGCGCA
    AAGCCTGCAG ATTCAGCATC TGATTCAGGG CGACCGGCCA CAAGCACCTT GAACCGCTTC
    TCAGCACTCC AGCAGTCCAC CCCTGCTGAG AGCTCGGAGT CCCGTCGCGT GGTGCAGAGG
    AGCAGCTCCA GCCGCGACAG GTCAGAAAAG GCTGGGGACA GAGGGGACCG GGAGTCACGT
    TCAGAGAAGA GCAGTGACCG TCTGGAGCGT CCTGATCGGG GAGAGCGGGG AGAGAGGAAC
    AGGTCTGCTG TCACCAAGAG GAGCTTCAGC AAAGAGACAG AGGACAGGAG CAGAGAACGG
    GAAAAGCAGA GTGGCCCTGA GGCCGTGCGT AAGACTGCTA GCATGTCAGA GGAACGGGAC
    AGGAGCCGAG AGACGATTAA ACAAGAGCCA GCACCTCCTG CGGCATCACC CAAACCCATG
    CTGTCAGAGG AGGAGCTGGA GAAGAAATCT AAGGCGATCA TAGAGGAATA TCTGCACATC
    AATGATATGA AGGAGGCCCT GCAGTGTGTG CAGGAGCTGG GCAGCCCCTC CTTGCTCTAC
    GTCTTCGTGC GGAATGGCAT TGAGTCCACG CTTGAGCGGA GCACGATCTC CCGCGAGCAC
    ATGGGGGTCC TGCTGTGCCA CCTGGTGAAG GCTGGCACGC TCTCCAAGGA GCAGTACTAT
    AAAGGGCTGC GGGAGATCCT GGAGATTGCA GAAGACATGG AGATTGATAT CCCGCACATC
    TGGCTGTATC TTGCCGAGCT CATAACACCT ATCCTGCAAG AGGAAGGCAT CCCCATGGAG
    GAGCTGTTCA GGGAGATAAC AAAGCCCCTG GTGCCCCTTG GGAAGGCCAC CACGCTGCTG
    GTTGAGGTGC TGGGCTTATT GTGCAAGGGC ATGAGCCAGA AGACTGCGGG CAAGCTGTGG
    CGGGATGGGG GACTGAGCTG GAAGGAATTC CTGCCTGAGG ACCAGGACAT CAACAAATTC
    GTCACAGAGC AGAAATTGGA GTACACGATG GGGGACAACT CGGACACGCC AAGCTGCAAG
    GAGCTGACCT CGGAGGAGCT GTGCAAGCAA ATGGACAAAC TGCTGAAGGA GAACTCGAAC
    AACCAAAGAA TATATGACTG GATTGAGGCC AACCTAAGTG AGCAGCAGGT CTCGTCCAAC
    ACATTTATCA GGGCCCTGAT GACATCCGTG TGCCATTCAG CCATCACCTT TGAGAACCCG
    TACCGTGTAG ACGCCCTGGT CATTAGCAAC CGGGCCAAGC TGCTGCAGAA GTACATGCGG
    GATGAGCAGA AGGAGCTTCA GGCACTCTAC GCCTTGCAGG CTTTGGTGGT GAAGTTGGAC
    CAGCCTCCAA ACCTGCTGCG GATGTTCTTT GATGCTCTCT ACGATGAGGA CGTCATCAAG
    GAGGAGGCCT TCTACAAGTG GGAGTCCAGC AAGGACCCGG CCGAGCAGCA GGGCAAAGGG
    GTGGCCCTGA AATCAGTGAC AGCGTTTTTC ACCTGGCTCC GGGAAGCAGA GGACGAGTCA
    GACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.