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EBI3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EBI3
Entrez Gene ID 10148
Full Name Epstein-Barr virus induced 3
Synonyms IL-27B,IL27B,IL35B
General protein information
Preferred Names

Epstein-Barr virus induced 3

Names

interleukin-27 subunit beta
EBV-induced gene 3 protein
Epstein-Barr virus induced gene 3
IL-27 subunit beta
IL27 subunit
IL35 subunit
cytokine receptor
epstein-Barr virus-induced gene 3 protein

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19p13.3

Summary This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008].
Disorder MIM:

605816


mRNA and Protein(s)

mRNA Protein Name
XM_011527619.1 XP_011525921.1 interleukin-27 subunit beta isoform X1
NM_005755.2 NP_005746.2 interleukin-27 subunit beta precursor
XM_011527619.2 XP_011525921.1 interleukin-27 subunit beta isoform X1



Homo sapiens (human) EBI3 NP_005746.2
Danio rerio (zebrafish) ebi3 NP_001106980.1
Pan troglodytes (chimpanzee) EBI3 XP_001138182.1
Canis lupus familiaris (dog) EBI3 XP_542161.3
Xenopus tropicalis (tropical clawed frog) ebi3 XP_002934057.1
Bos taurus (cattle) EBI3 NP_001093835.1
Mus musculus (house mouse) Ebi3 NP_056581.1
Rattus norvegicus (Norway rat) Ebi3 NP_001102891.1


Related articles in PubMed

Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway.
Zhang SA, Niyazi HE, Hong W, Tuluwengjiang GL, Zhang L, Zhang Y, Su WP, Bao YX
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317692237(2017 Mar)

The possible role of interleukin-35 and its therapeutic potential in pemphigus.
Tavakolpour S, Kheiry F, Mirsafaei HS, Akhlaghdoust M
International immunopharmacology4211-17(2017 Jan)

Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.
Chen Y, Wang CJ, Lin SH, Zhang M, Li SY, Xu F
Cytokine8123-7(2016 May)

Higher decidual EBI3 and HLA-G mRNA expression in preeclampsia: Cause or consequence of preeclampsia.
Prins JR, van der Hoorn ML, Keijser R, Ris-Stalpers C, van Beelen E, Afink GB, Claas FH, van der Post JA, Scherjon SA
Human immunology77(1)68-70(2016 Jan)

Elevated IL-35 in bone marrow of the patients with acute myeloid leukemia.
Wang J, Tao Q, Wang H, Wang Z, Wu F, Pan Y, Tao L, Xiong S, Wang Y, Zhai Z
Human immunology76(9)681-6(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

over-expression of EBI3 could reduce the apoptosis of Treg/CD4(+)T/CD8(+)T cells and prevent radiation-induced immunosuppression of cervical cancer HeLa cells by inhibiting the activation of PD-1/PD-L1 signaling pathway
Title: Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway.

this study introduces IL-35 as a new treatment for pemphigus
Title: The possible role of interleukin-35 and its therapeutic potential in pemphigus.

The plasma levels of interleukin-35 were significantly higher in the hepatocellular carcinoma patients than the controls.
Title: Plasma Level of Interleukin-35 as an Independent Prognostic Indicator in Hepatocellular Carcinoma.

Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) levels were significantly elevated in the patients with influenza infection.
Title: Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.

higher decidual mRNA expression in preeclampsia
Title: Higher decidual EBI3 and HLA-G mRNA expression in preeclampsia: Cause or consequence of preeclampsia.

The following EBI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu17365 NM_005755.2
Latest version!
Homo sapiens Epstein-Barr virus induced 3 (EBI3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu17365 XM_011527619.2
Latest version!
Homo sapiens Epstein-Barr virus induced 3 (EBI3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
View Old Accession Versions >>
OHu17365 XM_011527619.1 Homo sapiens Epstein-Barr virus induced 3 (EBI3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17365
Clone ID Related Accession (Same CDS sequence) XM_011527619.1 , NM_005755.2 , XM_011527619.2
Accession Version NM_005755.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-25
Organism Homo sapiens(Human)
Product interleukin-27 subunit beta precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L08187.1. On Jun 29, 2001 this sequence version replaced NM_005755.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.376789.1, BC015364.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGACCCCGC AGCTTCTCCT GGCCCTTGTC CTCTGGGCCA GCTGCCCGCC CTGCAGTGGA 
AGGAAAGGGC CCCCAGCAGC TCTGACACTG CCCCGGGTGC AATGCCGAGC CTCTCGGTAC
CCGATCGCCG TGGATTGCTC CTGGACCCTG CCGCCTGCTC CAAACTCCAC CAGCCCCGTG
TCCTTCATTG CCACGTACAG GCTCGGCATG GCTGCCCGGG GCCACAGCTG GCCCTGCCTG
CAGCAGACGC CAACGTCCAC CAGCTGCACC ATCACGGATG TCCAGCTGTT CTCCATGGCT
CCCTACGTGC TCAATGTCAC CGCCGTCCAC CCCTGGGGCT CCAGCAGCAG CTTCGTGCCT
TTCATAACAG AGCACATCAT CAAGCCCGAC CCTCCAGAAG GCGTGCGCCT AAGCCCCCTC
GCTGAGCGCC AGCTACAGGT GCAGTGGGAG CCTCCCGGGT CCTGGCCCTT CCCAGAGATC
TTCTCACTGA AGTACTGGAT CCGTTACAAG CGTCAGGGAG CTGCGCGCTT CCACCGGGTG
GGGCCCATTG AAGCCACGTC CTTCATCCTC AGGGCTGTGC GGCCCCGAGC CAGGTACTAC
GTCCAAGTGG CGGCTCAGGA CCTCACAGAC TACGGGGAAC TGAGTGACTG GAGTCTCCCC
GCCACTGCCA CAATGAGCCT GGGCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005746.2
CDS9..698
Exon (1)1..75
Translation

Target ORF information:

RefSeq Version NM_005755.2
Organism Homo sapiens(Human)
Definition Homo sapiens Epstein-Barr virus induced 3 (EBI3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005755.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCCGC AGCTTCTCCT GGCCCTTGTC CTCTGGGCCA GCTGCCCGCC CTGCAGTGGA 
AGGAAAGGGC CCCCAGCAGC TCTGACACTG CCCCGGGTGC AATGCCGAGC CTCTCGGTAC
CCGATCGCCG TGGATTGCTC CTGGACCCTG CCGCCTGCTC CAAACTCCAC CAGCCCCGTG
TCCTTCATTG CCACGTACAG GCTCGGCATG GCTGCCCGGG GCCACAGCTG GCCCTGCCTG
CAGCAGACGC CAACGTCCAC CAGCTGCACC ATCACGGATG TCCAGCTGTT CTCCATGGCT
CCCTACGTGC TCAATGTCAC CGCCGTCCAC CCCTGGGGCT CCAGCAGCAG CTTCGTGCCT
TTCATAACAG AGCACATCAT CAAGCCCGAC CCTCCAGAAG GCGTGCGCCT AAGCCCCCTC
GCTGAGCGCC AGCTACAGGT GCAGTGGGAG CCTCCCGGGT CCTGGCCCTT CCCAGAGATC
TTCTCACTGA AGTACTGGAT CCGTTACAAG CGTCAGGGAG CTGCGCGCTT CCACCGGGTG
GGGCCCATTG AAGCCACGTC CTTCATCCTC AGGGCTGTGC GGCCCCGAGC CAGGTACTAC
GTCCAAGTGG CGGCTCAGGA CCTCACAGAC TACGGGGAAC TGAGTGACTG GAGTCTCCCC
GCCACTGCCA CAATGAGCCT GGGCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17365
Clone ID Related Accession (Same CDS sequence) XM_011527619.1 , NM_005755.2 , XM_011527619.2
Accession Version XM_011527619.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product interleukin-27 subunit beta isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCCGC AGCTTCTCCT GGCCCTTGTC CTCTGGGCCA GCTGCCCGCC CTGCAGTGGA 
AGGAAAGGGC CCCCAGCAGC TCTGACACTG CCCCGGGTGC AATGCCGAGC CTCTCGGTAC
CCGATCGCCG TGGATTGCTC CTGGACCCTG CCGCCTGCTC CAAACTCCAC CAGCCCCGTG
TCCTTCATTG CCACGTACAG GCTCGGCATG GCTGCCCGGG GCCACAGCTG GCCCTGCCTG
CAGCAGACGC CAACGTCCAC CAGCTGCACC ATCACGGATG TCCAGCTGTT CTCCATGGCT
CCCTACGTGC TCAATGTCAC CGCCGTCCAC CCCTGGGGCT CCAGCAGCAG CTTCGTGCCT
TTCATAACAG AGCACATCAT CAAGCCCGAC CCTCCAGAAG GCGTGCGCCT AAGCCCCCTC
GCTGAGCGCC AGCTACAGGT GCAGTGGGAG CCTCCCGGGT CCTGGCCCTT CCCAGAGATC
TTCTCACTGA AGTACTGGAT CCGTTACAAG CGTCAGGGAG CTGCGCGCTT CCACCGGGTG
GGGCCCATTG AAGCCACGTC CTTCATCCTC AGGGCTGTGC GGCCCCGAGC CAGGTACTAC
GTCCAAGTGG CGGCTCAGGA CCTCACAGAC TACGGGGAAC TGAGTGACTG GAGTCTCCCC
GCCACTGCCA CAATGAGCCT GGGCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525921.1
Translation

Target ORF information:

RefSeq Version XM_011527619.1
Organism Homo sapiens(Human)
Definition Homo sapiens Epstein-Barr virus induced 3 (EBI3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011527619.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCCGC AGCTTCTCCT GGCCCTTGTC CTCTGGGCCA GCTGCCCGCC CTGCAGTGGA 
AGGAAAGGGC CCCCAGCAGC TCTGACACTG CCCCGGGTGC AATGCCGAGC CTCTCGGTAC
CCGATCGCCG TGGATTGCTC CTGGACCCTG CCGCCTGCTC CAAACTCCAC CAGCCCCGTG
TCCTTCATTG CCACGTACAG GCTCGGCATG GCTGCCCGGG GCCACAGCTG GCCCTGCCTG
CAGCAGACGC CAACGTCCAC CAGCTGCACC ATCACGGATG TCCAGCTGTT CTCCATGGCT
CCCTACGTGC TCAATGTCAC CGCCGTCCAC CCCTGGGGCT CCAGCAGCAG CTTCGTGCCT
TTCATAACAG AGCACATCAT CAAGCCCGAC CCTCCAGAAG GCGTGCGCCT AAGCCCCCTC
GCTGAGCGCC AGCTACAGGT GCAGTGGGAG CCTCCCGGGT CCTGGCCCTT CCCAGAGATC
TTCTCACTGA AGTACTGGAT CCGTTACAAG CGTCAGGGAG CTGCGCGCTT CCACCGGGTG
GGGCCCATTG AAGCCACGTC CTTCATCCTC AGGGCTGTGC GGCCCCGAGC CAGGTACTAC
GTCCAAGTGG CGGCTCAGGA CCTCACAGAC TACGGGGAAC TGAGTGACTG GAGTCTCCCC
GCCACTGCCA CAATGAGCCT GGGCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17365
Clone ID Related Accession (Same CDS sequence) XM_011527619.1 , NM_005755.2 , XM_011527619.2
Accession Version XM_011527619.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product interleukin-27 subunit beta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCCGC AGCTTCTCCT GGCCCTTGTC CTCTGGGCCA GCTGCCCGCC CTGCAGTGGA 
AGGAAAGGGC CCCCAGCAGC TCTGACACTG CCCCGGGTGC AATGCCGAGC CTCTCGGTAC
CCGATCGCCG TGGATTGCTC CTGGACCCTG CCGCCTGCTC CAAACTCCAC CAGCCCCGTG
TCCTTCATTG CCACGTACAG GCTCGGCATG GCTGCCCGGG GCCACAGCTG GCCCTGCCTG
CAGCAGACGC CAACGTCCAC CAGCTGCACC ATCACGGATG TCCAGCTGTT CTCCATGGCT
CCCTACGTGC TCAATGTCAC CGCCGTCCAC CCCTGGGGCT CCAGCAGCAG CTTCGTGCCT
TTCATAACAG AGCACATCAT CAAGCCCGAC CCTCCAGAAG GCGTGCGCCT AAGCCCCCTC
GCTGAGCGCC AGCTACAGGT GCAGTGGGAG CCTCCCGGGT CCTGGCCCTT CCCAGAGATC
TTCTCACTGA AGTACTGGAT CCGTTACAAG CGTCAGGGAG CTGCGCGCTT CCACCGGGTG
GGGCCCATTG AAGCCACGTC CTTCATCCTC AGGGCTGTGC GGCCCCGAGC CAGGTACTAC
GTCCAAGTGG CGGCTCAGGA CCTCACAGAC TACGGGGAAC TGAGTGACTG GAGTCTCCCC
GCCACTGCCA CAATGAGCCT GGGCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525921.1
CDS258..947
Translation

Target ORF information:

RefSeq Version XM_011527619.2
Organism Homo sapiens(Human)
Definition Homo sapiens Epstein-Barr virus induced 3 (EBI3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011527619.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACCCCGC AGCTTCTCCT GGCCCTTGTC CTCTGGGCCA GCTGCCCGCC CTGCAGTGGA 
AGGAAAGGGC CCCCAGCAGC TCTGACACTG CCCCGGGTGC AATGCCGAGC CTCTCGGTAC
CCGATCGCCG TGGATTGCTC CTGGACCCTG CCGCCTGCTC CAAACTCCAC CAGCCCCGTG
TCCTTCATTG CCACGTACAG GCTCGGCATG GCTGCCCGGG GCCACAGCTG GCCCTGCCTG
CAGCAGACGC CAACGTCCAC CAGCTGCACC ATCACGGATG TCCAGCTGTT CTCCATGGCT
CCCTACGTGC TCAATGTCAC CGCCGTCCAC CCCTGGGGCT CCAGCAGCAG CTTCGTGCCT
TTCATAACAG AGCACATCAT CAAGCCCGAC CCTCCAGAAG GCGTGCGCCT AAGCCCCCTC
GCTGAGCGCC AGCTACAGGT GCAGTGGGAG CCTCCCGGGT CCTGGCCCTT CCCAGAGATC
TTCTCACTGA AGTACTGGAT CCGTTACAAG CGTCAGGGAG CTGCGCGCTT CCACCGGGTG
GGGCCCATTG AAGCCACGTC CTTCATCCTC AGGGCTGTGC GGCCCCGAGC CAGGTACTAC
GTCCAAGTGG CGGCTCAGGA CCTCACAGAC TACGGGGAAC TGAGTGACTG GAGTCTCCCC
GCCACTGCCA CAATGAGCCT GGGCAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317692237(2017 Mar)
Zhang SA,Niyazi HE,Hong W,Tuluwengjiang GL,Zhang L,Zhang Y,Su WP,Bao YX


book

The possible role of interleukin-35 and its therapeutic potential in pemphigus.
International immunopharmacology4211-17(2017 Jan)
Tavakolpour S,Kheiry F,Mirsafaei HS,Akhlaghdoust M


book

Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.
Cytokine8123-7(2016 May)
Chen Y,Wang CJ,Lin SH,Zhang M,Li SY,Xu F


book

Higher decidual EBI3 and HLA-G mRNA expression in preeclampsia: Cause or consequence of preeclampsia.
Human immunology77(1)68-70(2016 Jan)
Prins JR,van der Hoorn ML,Keijser R,Ris-Stalpers C,van Beelen E,Afink GB,Claas FH,van der Post JA,Scherjon SA


book

Elevated IL-35 in bone marrow of the patients with acute myeloid leukemia.
Human immunology76(9)681-6(2015 Sep)
Wang J,Tao Q,Wang H,Wang Z,Wu F,Pan Y,Tao L,Xiong S,Wang Y,Zhai Z


 
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