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SLC25A13 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SLC25A13
Entrez Gene ID 10165
Full Name solute carrier family 25 member 13
Synonyms ARALAR2,CITRIN,CTLN2
General protein information
Preferred Names

solute carrier family 25 member 13

Names

calcium-binding mitochondrial carrier protein Aralar2
mitochondrial aspartate glutamate carrier 2
solute carrier family 25 (aspartate/glutamate carrier), member 13

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q21.3

Summary This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009].
Disorder MIM:

603859

Disorder Html: Citrullinemia, adult-onset type II, 603471 (3); Citrullinemia,

mRNA and Protein(s)

mRNA Protein Name
XM_011515727.2 XP_011514029.1 calcium-binding mitochondrial carrier protein Aralar2 isoform X3
XM_017011665.1 XP_016867154.1 calcium-binding mitochondrial carrier protein Aralar2 isoform X4
XM_006715831.3 XP_006715894.1 calcium-binding mitochondrial carrier protein Aralar2 isoform X1
XM_017011663.1 XP_016867152.1 calcium-binding mitochondrial carrier protein Aralar2 isoform X2
NM_001160210.1 NP_001153682.1 calcium-binding mitochondrial carrier protein Aralar2 isoform 1
NM_014251.2 NP_055066.1 calcium-binding mitochondrial carrier protein Aralar2 isoform 2
XM_017011664.1 XP_016867153.1 calcium-binding mitochondrial carrier protein Aralar2 isoform X4



Gallus gallus (chicken) SLC25A13 NP_001012967.1
Canis lupus familiaris (dog) SLC25A13 XP_852644.2
Bos taurus (cattle) SLC25A13 XP_002686709.3
Mus musculus (house mouse) Slc25a13 NP_001171043.1
Xenopus tropicalis (tropical clawed frog) slc25a13 NP_001107969.1
Macaca mulatta (rhesus monkey) SLC25A13 XP_001088340.2
Homo sapiens (human) SLC25A13 NP_055066.1
Pan troglodytes (chimpanzee) SLC25A13 XP_527824.3


Related articles in PubMed

SLC25A13 cDNA cloning analysis using peripheral blood lymphocytes facilitates the identification of a large deletion mutation: Molecular diagnosis of an infant with neonatal intrahepatic cholestasis caused by citrin deficiency.
Zeng HS, Lin WX, Zhao ST, Zhang ZH, Yang HW, Chen FP, Song YZ, Yin ZN
Molecular medicine reports14(6)5189-5194(2016 Dec)

[Analysis of clinical features and SLC25A13 gene mutations in a family affected with neonatal intrahepatic cholestasis].
Wang L, Cheng X, Yan L, Wei Y, Tang F, Dong X, Yuan Y, Xie Y
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics33(5)670-3(2016 Oct)

Identification of a Large SLC25A13 Deletion via Sophisticated Molecular Analyses Using Peripheral Blood Lymphocytes in an Infant with Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency (NICCD): A Clinical and Molecular Study.
Zheng QQ, Zhang ZH, Zeng HS, Lin WX, Yang HW, Yin ZN, Song YZ
BioMed research international20164124263(2016)

Calcium-induced conformational changes of the regulatory domain of human mitochondrial aspartate/glutamate carriers.
Thangaratnarajah C, Ruprecht JJ, Kunji ER
Nature communications55491(2014 Nov)

First Bulgarian case of citrin deficiency caused by one novel and one recurrent mutation in the SLC25A13 gene.
Avdjieva-Tzavella DM, Ivanova MB, Todorov TP, Todorova AP, Panteleeva EI, Tincheva SS, Lazarova EA, Kathom HM, Yaneva PG, Tincheva RS
Genetic counseling (Geneva, Switzerland)25(3)271-6(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the present study reported on clinical and molecular investigations of an infant with NICCD, who was confirmed to be a compound heterozygote of the c.851_854del4 mutation and a novel large deletion c.1019_1177+893del in the SLC25A13 gene.
Title: SLC25A13 cDNA cloning analysis using peripheral blood lymphocytes facilitates the identification of a large deletion mutation: Molecular diagnosis of an infant with neonatal intrahepatic cholestasis caused by citrin deficiency.

Data indicate that the patient was a compound heterozygote carrying c.851_854delGTAT and IVS16ins3kb mutations of the mitochondrial carrier citrin protein (SLC25A13) gene, which were respectively inherited from his mother and father.
Title: [Analysis of clinical features and SLC25A13 gene mutations in a family affected with neonatal intrahepatic cholestasis].

The large deletion enriched the SLC25A13 mutation spectrum, and its identification supported the concept that cDNA cloning analysis, along with other molecular tools such as semiquantitative PCR, could provide valuable clues, facilitating the identification of obscure SLC25A13 deletions.
Title: Identification of a Large SLC25A13 Deletion via Sophisticated Molecular Analyses Using Peripheral Blood Lymphocytes in an Infant with Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency (NICCD): A Clinical and Molecular Study.

Structure of the calcium bound and calcium free N- and C-terminal domains is described, elucidating the mechanism of calcium regulation.
Title: Calcium-induced conformational changes of the regulatory domain of human mitochondrial aspartate/glutamate carriers.

Mutation screening of the SLC25A13 gene revealed the compound heterozygous mutations c.1081C>T (p.R361*) and c.74C>A (p. A25E) which confirmed the diagnosis of NICCD. The nonsense mutation c.1081C>T (p.R361*) is novel.
Title: First Bulgarian case of citrin deficiency caused by one novel and one recurrent mutation in the SLC25A13 gene.

The following SLC25A13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC25A13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27423 NM_014251.2
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu63740 XM_017011664.1
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu63740 XM_017011665.1
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu63739 XM_011515727.2
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu26637 NM_001160210.1
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu41332 XM_006715831.3
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu97000 XM_017011663.1
Latest version!
Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27423
Clone ID Related Accession (Same CDS sequence) NM_014251.2
Accession Version NM_014251.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2028bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA118296.1, AC004458.1 and BC006566.2. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2008 this sequence version replaced NM_014251.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) lacks one-aa, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF118838.1, SRR1803614.153768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGGCCG CCAAGGTGGC TTTAACCAAG AGAGCAGATC CAGCTGAGCT TAGAACAATA 
TTTTTGAAGT ATGCAAGCAT TGAGAAAAAC GGTGAATTTT TCATGTCCCC CAATGACTTT
GTCACTCGAT ACTTGAACAT TTTTGGAGAA AGCCAGCCTA ATCCAAAGAC TGTGGAACTT
TTAAGTGGAG TGGTGGATCA GACCAAAGAT GGATTAATAT CTTTTCAAGA ATTTGTTGCC
TTTGAATCTG TCCTGTGTGC CCCTGATGCT TTGTTTATGG TAGCCTTTCA GCTGTTTGAC
AAAGCTGGCA AAGGAGAAGT AACTTTTGAG GATGTTAAGC AAGTTTTTGG ACAGACCACA
ATTCATCAAC ATATTCCATT TAACTGGGAT TCAGAATTTG TGCAACTACA TTTTGGAAAA
GAAAGAAAAA GACACCTGAC ATATGCGGAA TTTACTCAGT TTTTATTGGA AATACAACTG
GAGCACGCAA AGCAAGCCTT TGTGCAACGG GACAATGCTA GGACTGGGAG AGTCACAGCC
ATCGACTTCC GAGACATCAT GGTCACCATC CGCCCCCATG TCTTGACTCC TTTTGTAGAA
GAATGTCTAG TAGCTGCTGC TGGAGGTACC ACATCCCATC AAGTTAGTTT CTCCTATTTT
AATGGATTTA ATTCGCTCCT TAACAACATG GAACTCATTA GAAAGATCTA TAGCACTCTG
GCTGGCACCA GGAAAGATGT TGAAGTGACT AAGGAGGAGT TTGTTCTGGC AGCTCAGAAA
TTTGGTCAGG TTACACCCAT GGAAGTTGAC ATCTTGTTTC AGTTAGCAGA TTTATATGAG
CCAAGGGGAC GTATGACCTT AGCAGACATT GAACGGATTG CTCCTCTGGA AGAGGGAACT
CTGCCCTTTA ACTTGGCTGA GGCCCAGAGG CAGAAGGCCT CAGGTGATTC AGCTCGACCA
GTTCTTCTAC AAGTTGCAGA GTCGGCCTAC AGGTTTGGTC TGGGTTCTGT TGCTGGAGCT
GTTGGAGCCA CTGCTGTGTA TCCTATCGAT CTTGTAAAAA CTCGAATGCA GAACCAACGA
TCAACTGGCT CTTTTGTGGG AGAACTCATG TATAAAAACA GCTTTGACTG TTTTAAGAAA
GTGCTACGCT ATGAAGGCTT CTTTGGACTG TATAGAGGTC TGTTGCCACA GTTATTGGGA
GTTGCCCCAG AGAAGGCCAT AAAACTTACA GTGAACGATT TTGTGAGGGA TAAATTTATG
CACAAAGATG GTTCGGTCCC ACTTGCAGCA GAAATTCTTG CTGGAGGCTG CGCTGGAGGC
TCCCAGGTGA TTTTCACAAA TCCTTTAGAA ATCGTCAAGA TCCGTTTGCA AGTGGCAGGA
GAAATCACCA CTGGTCCTCG AGTCAGTGCT CTGTCTGTCG TGCGGGACCT GGGGTTTTTT
GGGATCTACA AGGGTGCCAA AGCATGCTTT CTGCGGGACA TTCCTTTCTC GGCCATCTAC
TTTCCGTGCT ATGCTCATGT GAAGGCTTCC TTTGCAAATG AAGATGGGCA GGTTAGCCCA
GGAAGCCTGC TCTTAGCTGG TGCCATAGCT GGTATGCCTG CAGCATCTTT AGTGACCCCT
GCTGATGTTA TCAAGACGAG ATTACAGGTG GCTGCCCGGG CTGGCCAAAC CACTTACAGC
GGAGTGATAG ACTGCTTTAG AAAGATACTG CGTGAAGAAG GACCAAAAGC TCTGTGGAAG
GGAGCTGGTG CTCGTGTATT TCGATCCTCA CCCCAGTTTG GTGTAACTTT GCTGACTTAC
GAATTGCTAC AGCGATGGTT CTACATTGAT TTTGGAGGAG TAAAACCCAT GGGATCAGAG
CCAGTTCCTA AATCCAGGAT CAACCTGCCT GCCCCGAATC CTGATCACGT TGGGGGCTAC
AAACTGGCAG TTGCTACATT TGCAGGGATT GAAAACAAAT TTGGACTTTA CCTACCTCTC
TTCAAGCCAT CAGTATCTAC CTCAAAGGCT ATTGGTGGAG GCCCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055066.1
CDS192..2219
Misc Feature(1)120..122
Misc Feature(2)195..197
Misc Feature(3)240..434
Misc Feature(4)246..434
Misc Feature(5)order(264..266,279..281,288..290,306..311,387..389,393..395,399..401,405..407,411..413,420..422)
Misc Feature(6)1167..1445
Misc Feature(7)1170..1961
Misc Feature(8)1176..1457
Misc Feature(9)1185..1238
Misc Feature(10)1248..1250
Misc Feature(11)1305..1307
Misc Feature(12)1368..1427
Misc Feature(13)1461..1724
Misc Feature(14)1467..1721
Misc Feature(15)1497..1538
Misc Feature(16)1548..1550
Misc Feature(17)1641..1643
Misc Feature(18)1641..1643
Misc Feature(19)1644..1703
Misc Feature(20)1737..2024
Misc Feature(21)1743..2009
Misc Feature(22)1761..1814
Misc Feature(23)1929..1931
Misc Feature(24)1932..1991
Misc Feature(25)2175..2177
Misc Feature(26)2187..2189
Exon (1)1..206
Exon (2)207..260
Exon (3)261..403
Exon (4)404..519
Exon (5)520..659
Exon (6)660..806
Exon (7)807..945
Exon (8)946..1039
Exon (9)1040..1124
Exon (10)1125..1209
Exon (11)1210..1368
Exon (12)1369..1421
Exon (13)1422..1502
Exon (14)1503..1643
Exon (15)1644..1782
Exon (16)1783..1941
Exon (17)1942..2032
Exon (18)2033..3190
Translation

Target ORF information:

RefSeq Version NM_014251.2
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014251.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGGCCG CCAAGGTGGC TTTAACCAAG AGAGCAGATC CAGCTGAGCT TAGAACAATA 
TTTTTGAAGT ATGCAAGCAT TGAGAAAAAC GGTGAATTTT TCATGTCCCC CAATGACTTT
GTCACTCGAT ACTTGAACAT TTTTGGAGAA AGCCAGCCTA ATCCAAAGAC TGTGGAACTT
TTAAGTGGAG TGGTGGATCA GACCAAAGAT GGATTAATAT CTTTTCAAGA ATTTGTTGCC
TTTGAATCTG TCCTGTGTGC CCCTGATGCT TTGTTTATGG TAGCCTTTCA GCTGTTTGAC
AAAGCTGGCA AAGGAGAAGT AACTTTTGAG GATGTTAAGC AAGTTTTTGG ACAGACCACA
ATTCATCAAC ATATTCCATT TAACTGGGAT TCAGAATTTG TGCAACTACA TTTTGGAAAA
GAAAGAAAAA GACACCTGAC ATATGCGGAA TTTACTCAGT TTTTATTGGA AATACAACTG
GAGCACGCAA AGCAAGCCTT TGTGCAACGG GACAATGCTA GGACTGGGAG AGTCACAGCC
ATCGACTTCC GAGACATCAT GGTCACCATC CGCCCCCATG TCTTGACTCC TTTTGTAGAA
GAATGTCTAG TAGCTGCTGC TGGAGGTACC ACATCCCATC AAGTTAGTTT CTCCTATTTT
AATGGATTTA ATTCGCTCCT TAACAACATG GAACTCATTA GAAAGATCTA TAGCACTCTG
GCTGGCACCA GGAAAGATGT TGAAGTGACT AAGGAGGAGT TTGTTCTGGC AGCTCAGAAA
TTTGGTCAGG TTACACCCAT GGAAGTTGAC ATCTTGTTTC AGTTAGCAGA TTTATATGAG
CCAAGGGGAC GTATGACCTT AGCAGACATT GAACGGATTG CTCCTCTGGA AGAGGGAACT
CTGCCCTTTA ACTTGGCTGA GGCCCAGAGG CAGAAGGCCT CAGGTGATTC AGCTCGACCA
GTTCTTCTAC AAGTTGCAGA GTCGGCCTAC AGGTTTGGTC TGGGTTCTGT TGCTGGAGCT
GTTGGAGCCA CTGCTGTGTA TCCTATCGAT CTTGTAAAAA CTCGAATGCA GAACCAACGA
TCAACTGGCT CTTTTGTGGG AGAACTCATG TATAAAAACA GCTTTGACTG TTTTAAGAAA
GTGCTACGCT ATGAAGGCTT CTTTGGACTG TATAGAGGTC TGTTGCCACA GTTATTGGGA
GTTGCCCCAG AGAAGGCCAT AAAACTTACA GTGAACGATT TTGTGAGGGA TAAATTTATG
CACAAAGATG GTTCGGTCCC ACTTGCAGCA GAAATTCTTG CTGGAGGCTG CGCTGGAGGC
TCCCAGGTGA TTTTCACAAA TCCTTTAGAA ATCGTCAAGA TCCGTTTGCA AGTGGCAGGA
GAAATCACCA CTGGTCCTCG AGTCAGTGCT CTGTCTGTCG TGCGGGACCT GGGGTTTTTT
GGGATCTACA AGGGTGCCAA AGCATGCTTT CTGCGGGACA TTCCTTTCTC GGCCATCTAC
TTTCCGTGCT ATGCTCATGT GAAGGCTTCC TTTGCAAATG AAGATGGGCA GGTTAGCCCA
GGAAGCCTGC TCTTAGCTGG TGCCATAGCT GGTATGCCTG CAGCATCTTT AGTGACCCCT
GCTGATGTTA TCAAGACGAG ATTACAGGTG GCTGCCCGGG CTGGCCAAAC CACTTACAGC
GGAGTGATAG ACTGCTTTAG AAAGATACTG CGTGAAGAAG GACCAAAAGC TCTGTGGAAG
GGAGCTGGTG CTCGTGTATT TCGATCCTCA CCCCAGTTTG GTGTAACTTT GCTGACTTAC
GAATTGCTAC AGCGATGGTT CTACATTGAT TTTGGAGGAG TAAAACCCAT GGGATCAGAG
CCAGTTCCTA AATCCAGGAT CAACCTGCCT GCCCCGAATC CTGATCACGT TGGGGGCTAC
AAACTGGCAG TTGCTACATT TGCAGGGATT GAAAACAAAT TTGGACTTTA CCTACCTCTC
TTCAAGCCAT CAGTATCTAC CTCAAAGGCT ATTGGTGGAG GCCCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63740
Clone ID Related Accession (Same CDS sequence) XM_017011665.1 , XM_017011664.1
Accession Version XM_017011665.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1176bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCTTAG CAGACATTGA ACGGATTGCT CCTCTGGAAG AGGGAACTCT GCCCTTTAAC 
TTGGCTGAGG CCCAGAGGCA GAAGGCCTCA GGTGATTCAG CTCGACCAGT TCTTCTACAA
GTTGCAGAGT CGGCCTACAG GTTTGGTCTG GGTTCTGTTG CTGGAGCTGT TGGAGCCACT
GCTGTGTATC CTATCGATCT TGTAAAAACT CGAATGCAGA ACCAACGATC AACTGGCTCT
TTTGTGGGAG AACTCATGTA TAAAAACAGC TTTGACTGTT TTAAGAAAGT GCTACGCTAT
GAAGGCTTCT TTGGACTGTA TAGAGGTCTG TTGCCACAGT TATTGGGAGT TGCCCCAGAG
AAGGCCATAA AACTTACAGT GAACGATTTT GTGAGGGATA AATTTATGCA CAAAGATGGT
TCGGTCCCAC TTGCAGCAGA AATTCTTGCT GGAGGCTGCG CTGGAGGCTC CCAGGTGATT
TTCACAAATC CTTTAGAAAT CGTCAAGATC CGTTTGCAAG TGGCAGGAGA AATCACCACT
GGTCCTCGAG TCAGTGCTCT GTCTGTCGTG CGGGACCTGG GGTTTTTTGG GATCTACAAG
GGTGCCAAAG CATGCTTTCT GCGGGACATT CCTTTCTCGG CCATCTACTT TCCGTGCTAT
GCTCATGTGA AGGCTTCCTT TGCAAATGAA GATGGGCAGG TTAGCCCAGG AAGCCTGCTC
TTAGCTGGTG CCATAGCTGG TATGCCTGCA GCATCTTTAG TGACCCCTGC TGATGTTATC
AAGACGAGAT TACAGGTGGC TGCCCGGGCT GGCCAAACCA CTTACAGCGG AGTGATAGAC
TGCTTTAGAA AGATACTGCG TGAAGAAGGA CCAAAAGCTC TGTGGAAGGG AGCTGGTGCT
CGTGTATTTC GATCCTCACC CCAGTTTGGT GTAACTTTGC TGACTTACGA ATTGCTACAG
CGATGGTTCT ACATTGATTT TGGAGGAGTA AAACCCATGG GATCAGAGCC AGTTCCTAAA
TCCAGGATCA ACCTGCCTGC CCCGAATCCT GATCACGTTG GGGGCTACAA ACTGGCAGTT
GCTACATTTG CAGGGATTGA AAACAAATTT GGACTTTACC TACCTCTCTT CAAGCCATCA
GTATCTACCT CAAAGGCTAT TGGTGGAGGC CCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867154.1
CDS807..1982
Misc Feature(1)933..1724
Misc Feature(2)939..1220
Misc Feature(3)1224..1487
Misc Feature(4)1500..1787
Translation

Target ORF information:

RefSeq Version XM_017011665.1
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017011665.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCTTAG CAGACATTGA ACGGATTGCT CCTCTGGAAG AGGGAACTCT GCCCTTTAAC 
TTGGCTGAGG CCCAGAGGCA GAAGGCCTCA GGTGATTCAG CTCGACCAGT TCTTCTACAA
GTTGCAGAGT CGGCCTACAG GTTTGGTCTG GGTTCTGTTG CTGGAGCTGT TGGAGCCACT
GCTGTGTATC CTATCGATCT TGTAAAAACT CGAATGCAGA ACCAACGATC AACTGGCTCT
TTTGTGGGAG AACTCATGTA TAAAAACAGC TTTGACTGTT TTAAGAAAGT GCTACGCTAT
GAAGGCTTCT TTGGACTGTA TAGAGGTCTG TTGCCACAGT TATTGGGAGT TGCCCCAGAG
AAGGCCATAA AACTTACAGT GAACGATTTT GTGAGGGATA AATTTATGCA CAAAGATGGT
TCGGTCCCAC TTGCAGCAGA AATTCTTGCT GGAGGCTGCG CTGGAGGCTC CCAGGTGATT
TTCACAAATC CTTTAGAAAT CGTCAAGATC CGTTTGCAAG TGGCAGGAGA AATCACCACT
GGTCCTCGAG TCAGTGCTCT GTCTGTCGTG CGGGACCTGG GGTTTTTTGG GATCTACAAG
GGTGCCAAAG CATGCTTTCT GCGGGACATT CCTTTCTCGG CCATCTACTT TCCGTGCTAT
GCTCATGTGA AGGCTTCCTT TGCAAATGAA GATGGGCAGG TTAGCCCAGG AAGCCTGCTC
TTAGCTGGTG CCATAGCTGG TATGCCTGCA GCATCTTTAG TGACCCCTGC TGATGTTATC
AAGACGAGAT TACAGGTGGC TGCCCGGGCT GGCCAAACCA CTTACAGCGG AGTGATAGAC
TGCTTTAGAA AGATACTGCG TGAAGAAGGA CCAAAAGCTC TGTGGAAGGG AGCTGGTGCT
CGTGTATTTC GATCCTCACC CCAGTTTGGT GTAACTTTGC TGACTTACGA ATTGCTACAG
CGATGGTTCT ACATTGATTT TGGAGGAGTA AAACCCATGG GATCAGAGCC AGTTCCTAAA
TCCAGGATCA ACCTGCCTGC CCCGAATCCT GATCACGTTG GGGGCTACAA ACTGGCAGTT
GCTACATTTG CAGGGATTGA AAACAAATTT GGACTTTACC TACCTCTCTT CAAGCCATCA
GTATCTACCT CAAAGGCTAT TGGTGGAGGC CCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63740
Clone ID Related Accession (Same CDS sequence) XM_017011665.1 , XM_017011664.1
Accession Version XM_017011664.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1176bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCTTAG CAGACATTGA ACGGATTGCT CCTCTGGAAG AGGGAACTCT GCCCTTTAAC 
TTGGCTGAGG CCCAGAGGCA GAAGGCCTCA GGTGATTCAG CTCGACCAGT TCTTCTACAA
GTTGCAGAGT CGGCCTACAG GTTTGGTCTG GGTTCTGTTG CTGGAGCTGT TGGAGCCACT
GCTGTGTATC CTATCGATCT TGTAAAAACT CGAATGCAGA ACCAACGATC AACTGGCTCT
TTTGTGGGAG AACTCATGTA TAAAAACAGC TTTGACTGTT TTAAGAAAGT GCTACGCTAT
GAAGGCTTCT TTGGACTGTA TAGAGGTCTG TTGCCACAGT TATTGGGAGT TGCCCCAGAG
AAGGCCATAA AACTTACAGT GAACGATTTT GTGAGGGATA AATTTATGCA CAAAGATGGT
TCGGTCCCAC TTGCAGCAGA AATTCTTGCT GGAGGCTGCG CTGGAGGCTC CCAGGTGATT
TTCACAAATC CTTTAGAAAT CGTCAAGATC CGTTTGCAAG TGGCAGGAGA AATCACCACT
GGTCCTCGAG TCAGTGCTCT GTCTGTCGTG CGGGACCTGG GGTTTTTTGG GATCTACAAG
GGTGCCAAAG CATGCTTTCT GCGGGACATT CCTTTCTCGG CCATCTACTT TCCGTGCTAT
GCTCATGTGA AGGCTTCCTT TGCAAATGAA GATGGGCAGG TTAGCCCAGG AAGCCTGCTC
TTAGCTGGTG CCATAGCTGG TATGCCTGCA GCATCTTTAG TGACCCCTGC TGATGTTATC
AAGACGAGAT TACAGGTGGC TGCCCGGGCT GGCCAAACCA CTTACAGCGG AGTGATAGAC
TGCTTTAGAA AGATACTGCG TGAAGAAGGA CCAAAAGCTC TGTGGAAGGG AGCTGGTGCT
CGTGTATTTC GATCCTCACC CCAGTTTGGT GTAACTTTGC TGACTTACGA ATTGCTACAG
CGATGGTTCT ACATTGATTT TGGAGGAGTA AAACCCATGG GATCAGAGCC AGTTCCTAAA
TCCAGGATCA ACCTGCCTGC CCCGAATCCT GATCACGTTG GGGGCTACAA ACTGGCAGTT
GCTACATTTG CAGGGATTGA AAACAAATTT GGACTTTACC TACCTCTCTT CAAGCCATCA
GTATCTACCT CAAAGGCTAT TGGTGGAGGC CCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867153.1
CDS930..2105
Misc Feature(1)1056..1847
Misc Feature(2)1062..1343
Misc Feature(3)1347..1610
Misc Feature(4)1623..1910
Translation

Target ORF information:

RefSeq Version XM_017011664.1
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017011664.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCTTAG CAGACATTGA ACGGATTGCT CCTCTGGAAG AGGGAACTCT GCCCTTTAAC 
TTGGCTGAGG CCCAGAGGCA GAAGGCCTCA GGTGATTCAG CTCGACCAGT TCTTCTACAA
GTTGCAGAGT CGGCCTACAG GTTTGGTCTG GGTTCTGTTG CTGGAGCTGT TGGAGCCACT
GCTGTGTATC CTATCGATCT TGTAAAAACT CGAATGCAGA ACCAACGATC AACTGGCTCT
TTTGTGGGAG AACTCATGTA TAAAAACAGC TTTGACTGTT TTAAGAAAGT GCTACGCTAT
GAAGGCTTCT TTGGACTGTA TAGAGGTCTG TTGCCACAGT TATTGGGAGT TGCCCCAGAG
AAGGCCATAA AACTTACAGT GAACGATTTT GTGAGGGATA AATTTATGCA CAAAGATGGT
TCGGTCCCAC TTGCAGCAGA AATTCTTGCT GGAGGCTGCG CTGGAGGCTC CCAGGTGATT
TTCACAAATC CTTTAGAAAT CGTCAAGATC CGTTTGCAAG TGGCAGGAGA AATCACCACT
GGTCCTCGAG TCAGTGCTCT GTCTGTCGTG CGGGACCTGG GGTTTTTTGG GATCTACAAG
GGTGCCAAAG CATGCTTTCT GCGGGACATT CCTTTCTCGG CCATCTACTT TCCGTGCTAT
GCTCATGTGA AGGCTTCCTT TGCAAATGAA GATGGGCAGG TTAGCCCAGG AAGCCTGCTC
TTAGCTGGTG CCATAGCTGG TATGCCTGCA GCATCTTTAG TGACCCCTGC TGATGTTATC
AAGACGAGAT TACAGGTGGC TGCCCGGGCT GGCCAAACCA CTTACAGCGG AGTGATAGAC
TGCTTTAGAA AGATACTGCG TGAAGAAGGA CCAAAAGCTC TGTGGAAGGG AGCTGGTGCT
CGTGTATTTC GATCCTCACC CCAGTTTGGT GTAACTTTGC TGACTTACGA ATTGCTACAG
CGATGGTTCT ACATTGATTT TGGAGGAGTA AAACCCATGG GATCAGAGCC AGTTCCTAAA
TCCAGGATCA ACCTGCCTGC CCCGAATCCT GATCACGTTG GGGGCTACAA ACTGGCAGTT
GCTACATTTG CAGGGATTGA AAACAAATTT GGACTTTACC TACCTCTCTT CAAGCCATCA
GTATCTACCT CAAAGGCTAT TGGTGGAGGC CCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63739
Clone ID Related Accession (Same CDS sequence) XM_011515727.2
Accession Version XM_011515727.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCAACAC ATGGCAAAGT AAAGGGGGAA GGAGATTTTC TGCTGCATGT GGCTTTAACC 
AAGAGAGCAG ATCCAGCTGA GCTTAGAACA ATATTTTTGA AGTATGCAAG CATTGAGAAA
AACGGTGAAT TTTTCATGTC CCCCAATGAC TTTGTCACTC GATACTTGAA CATTTTTGGA
GAAAGCCAGC CTAATCCAAA GACTGTGGAA CTTTTAAGTG GAGTGGTGGA TCAGACCAAA
GATGGATTAA TATCTTTTCA AGAATTTGTT GCCTTTGAAT CTGTCCTGTG TGCCCCTGAT
GCTTTGTTTA TGGTAGCCTT TCAGCTGTTT GACAAAGCTG GCAAAGGAGA AGTAACTTTT
GAGGATGTTA AGCAAGTTTT TGGACAGACC ACAATTCATC AACATATTCC ATTTAACTGG
GATTCAGAAT TTGTGCAACT ACATTTTGGA AAAGAAAGAA AAAGACACCT GACATATGCG
GAATTTACTC AGTTTTTATT GGAAATACAA CTGGAGCACG CAAAGCAAGC CTTTGTGCAA
CGGGACAATG CTAGGACTGG GAGAGTCACA GCCATCGACT TCCGAGACAT CATGGTCACC
ATCCGCCCCC ATGTCTTGAC TCCTTTTGTA GAAGAATGTC TAGTAGCTGC TGCTGGAGGT
ACCACATCCC ATCAAGTTAG TTTCTCCTAT TTTAATGGAT TTAATTCGCT CCTTAACAAC
ATGGAACTCA TTAGAAAGAT CTATAGCACT CTGGCTGGCA CCAGGAAAGA TGTTGAAGTG
ACTAAGGAGG AGTTTGTTCT GGCAGCTCAG AAATTTGGTC AGGTTACACC CATGGAAGTT
GACATCTTGT TTCAGTTAGC AGATTTATAT GAGCCAAGGG GACGTATGAC CTTAGCAGAC
ATTGAACGGA TTGCTCCTCT GGAAGAGGGA ACTCTGCCCT TTAACTTGGC TGAGGCCCAG
AGGCAGAAGG CCTCAGGTGA TTCAGCTCGA CCAGTTCTTC TACAAGTTGC AGAGTCGGCC
TACAGGTTTG GTCTGGGTTC TGTTGCTGGA GCTGTTGGAG CCACTGCTGT GTATCCTATC
GATCTTGTAA AAACTCGAAT GCAGAACCAA CGATCAACTG GCTCTTTTGT GGGAGAACTC
ATGTATAAAA ACAGCTTTGA CTGTTTTAAG AAAGTGCTAC GCTATGAAGG CTTCTTTGGA
CTGTATAGAG GTCTGTTGCC ACAGTTATTG GGAGTTGCCC CAGAGAAGGC CATAAAACTT
ACAGTGAACG ATTTTGTGAG GGATAAATTT ATGCACAAAG ATGGTTCGGT CCCACTTGCA
GCAGAAATTC TTGCTGGAGG CTGCATCCTG CACAAGGGCA GAGTCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514029.1
CDS283..1653
Misc Feature(1)364..558
Misc Feature(2)370..558
Misc Feature(3)order(388..390,403..405,412..414,430..435,511..513,517..519,523..525,529..531,535..537,544..546)
Misc Feature(4)1300..1581
Translation

Target ORF information:

RefSeq Version XM_011515727.2
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515727.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCAACAC ATGGCAAAGT AAAGGGGGAA GGAGATTTTC TGCTGCATGT GGCTTTAACC 
AAGAGAGCAG ATCCAGCTGA GCTTAGAACA ATATTTTTGA AGTATGCAAG CATTGAGAAA
AACGGTGAAT TTTTCATGTC CCCCAATGAC TTTGTCACTC GATACTTGAA CATTTTTGGA
GAAAGCCAGC CTAATCCAAA GACTGTGGAA CTTTTAAGTG GAGTGGTGGA TCAGACCAAA
GATGGATTAA TATCTTTTCA AGAATTTGTT GCCTTTGAAT CTGTCCTGTG TGCCCCTGAT
GCTTTGTTTA TGGTAGCCTT TCAGCTGTTT GACAAAGCTG GCAAAGGAGA AGTAACTTTT
GAGGATGTTA AGCAAGTTTT TGGACAGACC ACAATTCATC AACATATTCC ATTTAACTGG
GATTCAGAAT TTGTGCAACT ACATTTTGGA AAAGAAAGAA AAAGACACCT GACATATGCG
GAATTTACTC AGTTTTTATT GGAAATACAA CTGGAGCACG CAAAGCAAGC CTTTGTGCAA
CGGGACAATG CTAGGACTGG GAGAGTCACA GCCATCGACT TCCGAGACAT CATGGTCACC
ATCCGCCCCC ATGTCTTGAC TCCTTTTGTA GAAGAATGTC TAGTAGCTGC TGCTGGAGGT
ACCACATCCC ATCAAGTTAG TTTCTCCTAT TTTAATGGAT TTAATTCGCT CCTTAACAAC
ATGGAACTCA TTAGAAAGAT CTATAGCACT CTGGCTGGCA CCAGGAAAGA TGTTGAAGTG
ACTAAGGAGG AGTTTGTTCT GGCAGCTCAG AAATTTGGTC AGGTTACACC CATGGAAGTT
GACATCTTGT TTCAGTTAGC AGATTTATAT GAGCCAAGGG GACGTATGAC CTTAGCAGAC
ATTGAACGGA TTGCTCCTCT GGAAGAGGGA ACTCTGCCCT TTAACTTGGC TGAGGCCCAG
AGGCAGAAGG CCTCAGGTGA TTCAGCTCGA CCAGTTCTTC TACAAGTTGC AGAGTCGGCC
TACAGGTTTG GTCTGGGTTC TGTTGCTGGA GCTGTTGGAG CCACTGCTGT GTATCCTATC
GATCTTGTAA AAACTCGAAT GCAGAACCAA CGATCAACTG GCTCTTTTGT GGGAGAACTC
ATGTATAAAA ACAGCTTTGA CTGTTTTAAG AAAGTGCTAC GCTATGAAGG CTTCTTTGGA
CTGTATAGAG GTCTGTTGCC ACAGTTATTG GGAGTTGCCC CAGAGAAGGC CATAAAACTT
ACAGTGAACG ATTTTGTGAG GGATAAATTT ATGCACAAAG ATGGTTCGGT CCCACTTGCA
GCAGAAATTC TTGCTGGAGG CTGCATCCTG CACAAGGGCA GAGTCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26637
Clone ID Related Accession (Same CDS sequence) NM_001160210.1
Accession Version NM_001160210.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA118296.1, DA696723.1 and AJ496569.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ496569.1, SRR1803612.58610.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGGCCG CCAAGGTGGC TTTAACCAAG AGAGCAGATC CAGCTGAGCT TAGAACAATA 
TTTTTGAAGT ATGCAAGCAT TGAGAAAAAC GGTGAATTTT TCATGTCCCC CAATGACTTT
GTCACTCGAT ACTTGAACAT TTTTGGAGAA AGCCAGCCTA ATCCAAAGAC TGTGGAACTT
TTAAGTGGAG TGGTGGATCA GACCAAAGAT GGATTAATAT CTTTTCAAGA ATTTGTTGCC
TTTGAATCTG TCCTGTGTGC CCCTGATGCT TTGTTTATGG TAGCCTTTCA GCTGTTTGAC
AAAGCTGGCA AAGGAGAAGT AACTTTTGAG GATGTTAAGC AAGTTTTTGG ACAGACCACA
ATTCATCAAC ATATTCCATT TAACTGGGAT TCAGAATTTG TGCAACTACA TTTTGGAAAA
GAAAGAAAAA GACACCTGAC ATATGCGGAA TTTACTCAGT TTTTATTGGA AATACAACTG
GAGCACGCAA AGCAAGCCTT TGTGCAACGG GACAATGCTA GGACTGGGAG AGTCACAGCC
ATCGACTTCC GAGACATCAT GGTCACCATC CGCCCCCATG TCTTGACTCC TTTTGTAGAA
GAATGTCTAG TAGCTGCTGC TGGAGGTACC ACATCCCATC AAGTTAGTTT CTCCTATTTT
AATGGATTTA ATTCGCTCCT TAACAACATG GAACTCATTA GAAAGATCTA TAGCACTCTG
GCTGGCACCA GGAAAGATGT TGAAGTGACT AAGGAGGAGT TTGTTCTGGC AGCTCAGAAA
TTTGGTCAGG TTACACCCAT GGAAGTTGAC ATCTTGTTTC AGTTAGCAGA TTTATATGAG
CCAAGGGGAC GTATGACCTT AGCAGACATT GAACGGATTG CTCCTCTGGA AGAGGGAACT
CTGCCCTTTA ACTTGGCTGA GGCCCAGAGG CAGCAGAAGG CCTCAGGTGA TTCAGCTCGA
CCAGTTCTTC TACAAGTTGC AGAGTCGGCC TACAGGTTTG GTCTGGGTTC TGTTGCTGGA
GCTGTTGGAG CCACTGCTGT GTATCCTATC GATCTTGTAA AAACTCGAAT GCAGAACCAA
CGATCAACTG GCTCTTTTGT GGGAGAACTC ATGTATAAAA ACAGCTTTGA CTGTTTTAAG
AAAGTGCTAC GCTATGAAGG CTTCTTTGGA CTGTATAGAG GTCTGTTGCC ACAGTTATTG
GGAGTTGCCC CAGAGAAGGC CATAAAACTT ACAGTGAACG ATTTTGTGAG GGATAAATTT
ATGCACAAAG ATGGTTCGGT CCCACTTGCA GCAGAAATTC TTGCTGGAGG CTGCGCTGGA
GGCTCCCAGG TGATTTTCAC AAATCCTTTA GAAATCGTCA AGATCCGTTT GCAAGTGGCA
GGAGAAATCA CCACTGGTCC TCGAGTCAGT GCTCTGTCTG TCGTGCGGGA CCTGGGGTTT
TTTGGGATCT ACAAGGGTGC CAAAGCATGC TTTCTGCGGG ACATTCCTTT CTCGGCCATC
TACTTTCCGT GCTATGCTCA TGTGAAGGCT TCCTTTGCAA ATGAAGATGG GCAGGTTAGC
CCAGGAAGCC TGCTCTTAGC TGGTGCCATA GCTGGTATGC CTGCAGCATC TTTAGTGACC
CCTGCTGATG TTATCAAGAC GAGATTACAG GTGGCTGCCC GGGCTGGCCA AACCACTTAC
AGCGGAGTGA TAGACTGCTT TAGAAAGATA CTGCGTGAAG AAGGACCAAA AGCTCTGTGG
AAGGGAGCTG GTGCTCGTGT ATTTCGATCC TCACCCCAGT TTGGTGTAAC TTTGCTGACT
TACGAATTGC TACAGCGATG GTTCTACATT GATTTTGGAG GAGTAAAACC CATGGGATCA
GAGCCAGTTC CTAAATCCAG GATCAACCTG CCTGCCCCGA ATCCTGATCA CGTTGGGGGC
TACAAACTGG CAGTTGCTAC ATTTGCAGGG ATTGAAAACA AATTTGGACT TTACCTACCT
CTCTTCAAGC CATCAGTATC TACCTCAAAG GCTATTGGTG GAGGCCCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153682.1
CDS192..2222
Misc Feature(1)120..122
Misc Feature(2)195..197
Misc Feature(3)240..434
Misc Feature(4)246..434
Misc Feature(5)order(264..266,279..281,288..290,306..311,387..389,393..395,399..401,405..407,411..413,420..422)
Misc Feature(6)1170..1448
Misc Feature(7)1173..1964
Misc Feature(8)1179..1460
Misc Feature(9)1188..1241
Misc Feature(10)1251..1253
Misc Feature(11)1308..1310
Misc Feature(12)1371..1430
Misc Feature(13)1464..1727
Misc Feature(14)1470..1724
Misc Feature(15)1500..1541
Misc Feature(16)1551..1553
Misc Feature(17)1644..1646
Misc Feature(18)1644..1646
Misc Feature(19)1647..1706
Misc Feature(20)1740..2027
Misc Feature(21)1746..2012
Misc Feature(22)1764..1817
Misc Feature(23)1932..1934
Misc Feature(24)1935..1994
Misc Feature(25)2178..2180
Misc Feature(26)2190..2192
Exon (1)1..206
Exon (2)207..260
Exon (3)261..403
Exon (4)404..519
Exon (5)520..659
Exon (6)660..806
Exon (7)807..945
Exon (8)946..1039
Exon (9)1040..1124
Exon (10)1125..1212
Exon (11)1213..1371
Exon (12)1372..1424
Exon (13)1425..1505
Exon (14)1506..1646
Exon (15)1647..1785
Exon (16)1786..1944
Exon (17)1945..2035
Exon (18)2036..3193
Translation

Target ORF information:

RefSeq Version NM_001160210.1
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001160210.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGGCCG CCAAGGTGGC TTTAACCAAG AGAGCAGATC CAGCTGAGCT TAGAACAATA 
TTTTTGAAGT ATGCAAGCAT TGAGAAAAAC GGTGAATTTT TCATGTCCCC CAATGACTTT
GTCACTCGAT ACTTGAACAT TTTTGGAGAA AGCCAGCCTA ATCCAAAGAC TGTGGAACTT
TTAAGTGGAG TGGTGGATCA GACCAAAGAT GGATTAATAT CTTTTCAAGA ATTTGTTGCC
TTTGAATCTG TCCTGTGTGC CCCTGATGCT TTGTTTATGG TAGCCTTTCA GCTGTTTGAC
AAAGCTGGCA AAGGAGAAGT AACTTTTGAG GATGTTAAGC AAGTTTTTGG ACAGACCACA
ATTCATCAAC ATATTCCATT TAACTGGGAT TCAGAATTTG TGCAACTACA TTTTGGAAAA
GAAAGAAAAA GACACCTGAC ATATGCGGAA TTTACTCAGT TTTTATTGGA AATACAACTG
GAGCACGCAA AGCAAGCCTT TGTGCAACGG GACAATGCTA GGACTGGGAG AGTCACAGCC
ATCGACTTCC GAGACATCAT GGTCACCATC CGCCCCCATG TCTTGACTCC TTTTGTAGAA
GAATGTCTAG TAGCTGCTGC TGGAGGTACC ACATCCCATC AAGTTAGTTT CTCCTATTTT
AATGGATTTA ATTCGCTCCT TAACAACATG GAACTCATTA GAAAGATCTA TAGCACTCTG
GCTGGCACCA GGAAAGATGT TGAAGTGACT AAGGAGGAGT TTGTTCTGGC AGCTCAGAAA
TTTGGTCAGG TTACACCCAT GGAAGTTGAC ATCTTGTTTC AGTTAGCAGA TTTATATGAG
CCAAGGGGAC GTATGACCTT AGCAGACATT GAACGGATTG CTCCTCTGGA AGAGGGAACT
CTGCCCTTTA ACTTGGCTGA GGCCCAGAGG CAGCAGAAGG CCTCAGGTGA TTCAGCTCGA
CCAGTTCTTC TACAAGTTGC AGAGTCGGCC TACAGGTTTG GTCTGGGTTC TGTTGCTGGA
GCTGTTGGAG CCACTGCTGT GTATCCTATC GATCTTGTAA AAACTCGAAT GCAGAACCAA
CGATCAACTG GCTCTTTTGT GGGAGAACTC ATGTATAAAA ACAGCTTTGA CTGTTTTAAG
AAAGTGCTAC GCTATGAAGG CTTCTTTGGA CTGTATAGAG GTCTGTTGCC ACAGTTATTG
GGAGTTGCCC CAGAGAAGGC CATAAAACTT ACAGTGAACG ATTTTGTGAG GGATAAATTT
ATGCACAAAG ATGGTTCGGT CCCACTTGCA GCAGAAATTC TTGCTGGAGG CTGCGCTGGA
GGCTCCCAGG TGATTTTCAC AAATCCTTTA GAAATCGTCA AGATCCGTTT GCAAGTGGCA
GGAGAAATCA CCACTGGTCC TCGAGTCAGT GCTCTGTCTG TCGTGCGGGA CCTGGGGTTT
TTTGGGATCT ACAAGGGTGC CAAAGCATGC TTTCTGCGGG ACATTCCTTT CTCGGCCATC
TACTTTCCGT GCTATGCTCA TGTGAAGGCT TCCTTTGCAA ATGAAGATGG GCAGGTTAGC
CCAGGAAGCC TGCTCTTAGC TGGTGCCATA GCTGGTATGC CTGCAGCATC TTTAGTGACC
CCTGCTGATG TTATCAAGAC GAGATTACAG GTGGCTGCCC GGGCTGGCCA AACCACTTAC
AGCGGAGTGA TAGACTGCTT TAGAAAGATA CTGCGTGAAG AAGGACCAAA AGCTCTGTGG
AAGGGAGCTG GTGCTCGTGT ATTTCGATCC TCACCCCAGT TTGGTGTAAC TTTGCTGACT
TACGAATTGC TACAGCGATG GTTCTACATT GATTTTGGAG GAGTAAAACC CATGGGATCA
GAGCCAGTTC CTAAATCCAG GATCAACCTG CCTGCCCCGA ATCCTGATCA CGTTGGGGGC
TACAAACTGG CAGTTGCTAC ATTTGCAGGG ATTGAAAACA AATTTGGACT TTACCTACCT
CTCTTCAAGC CATCAGTATC TACCTCAAAG GCTATTGGTG GAGGCCCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41332
Clone ID Related Accession (Same CDS sequence) XM_006715831.3
Accession Version XM_006715831.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2061bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGCCAACAC ATGGCAAAGT AAAGGGGGAA GGAGATTTTC TGCTGCATGT GGCTTTAACC 
AAGAGAGCAG ATCCAGCTGA GCTTAGAACA ATATTTTTGA AGTATGCAAG CATTGAGAAA
AACGGTGAAT TTTTCATGTC CCCCAATGAC TTTGTCACTC GATACTTGAA CATTTTTGGA
GAAAGCCAGC CTAATCCAAA GACTGTGGAA CTTTTAAGTG GAGTGGTGGA TCAGACCAAA
GATGGATTAA TATCTTTTCA AGAATTTGTT GCCTTTGAAT CTGTCCTGTG TGCCCCTGAT
GCTTTGTTTA TGGTAGCCTT TCAGCTGTTT GACAAAGCTG GCAAAGGAGA AGTAACTTTT
GAGGATGTTA AGCAAGTTTT TGGACAGACC ACAATTCATC AACATATTCC ATTTAACTGG
GATTCAGAAT TTGTGCAACT ACATTTTGGA AAAGAAAGAA AAAGACACCT GACATATGCG
GAATTTACTC AGTTTTTATT GGAAATACAA CTGGAGCACG CAAAGCAAGC CTTTGTGCAA
CGGGACAATG CTAGGACTGG GAGAGTCACA GCCATCGACT TCCGAGACAT CATGGTCACC
ATCCGCCCCC ATGTCTTGAC TCCTTTTGTA GAAGAATGTC TAGTAGCTGC TGCTGGAGGT
ACCACATCCC ATCAAGTTAG TTTCTCCTAT TTTAATGGAT TTAATTCGCT CCTTAACAAC
ATGGAACTCA TTAGAAAGAT CTATAGCACT CTGGCTGGCA CCAGGAAAGA TGTTGAAGTG
ACTAAGGAGG AGTTTGTTCT GGCAGCTCAG AAATTTGGTC AGGTTACACC CATGGAAGTT
GACATCTTGT TTCAGTTAGC AGATTTATAT GAGCCAAGGG GACGTATGAC CTTAGCAGAC
ATTGAACGGA TTGCTCCTCT GGAAGAGGGA ACTCTGCCCT TTAACTTGGC TGAGGCCCAG
AGGCAGAAGG CCTCAGGTGA TTCAGCTCGA CCAGTTCTTC TACAAGTTGC AGAGTCGGCC
TACAGGTTTG GTCTGGGTTC TGTTGCTGGA GCTGTTGGAG CCACTGCTGT GTATCCTATC
GATCTTGTAA AAACTCGAAT GCAGAACCAA CGATCAACTG GCTCTTTTGT GGGAGAACTC
ATGTATAAAA ACAGCTTTGA CTGTTTTAAG AAAGTGCTAC GCTATGAAGG CTTCTTTGGA
CTGTATAGAG GTCTGTTGCC ACAGTTATTG GGAGTTGCCC CAGAGAAGGC CATAAAACTT
ACAGTGAACG ATTTTGTGAG GGATAAATTT ATGCACAAAG ATGGTTCGGT CCCACTTGCA
GCAGAAATTC TTGCTGGAGG CTGCGCTGGA GGCTCCCAGG TGATTTTCAC AAATCCTTTA
GAAATCGTCA AGATCCGTTT GCAAGTGGCA GGAGAAATCA CCACTGGTCC TCGAGTCAGT
GCTCTGTCTG TCGTGCGGGA CCTGGGGTTT TTTGGGATCT ACAAGGGTGC CAAAGCATGC
TTTCTGCGGG ACATTCCTTT CTCGGCCATC TACTTTCCGT GCTATGCTCA TGTGAAGGCT
TCCTTTGCAA ATGAAGATGG GCAGGTTAGC CCAGGAAGCC TGCTCTTAGC TGGTGCCATA
GCTGGTATGC CTGCAGCATC TTTAGTGACC CCTGCTGATG TTATCAAGAC GAGATTACAG
GTGGCTGCCC GGGCTGGCCA AACCACTTAC AGCGGAGTGA TAGACTGCTT TAGAAAGATA
CTGCGTGAAG AAGGACCAAA AGCTCTGTGG AAGGGAGCTG GTGCTCGTGT ATTTCGATCC
TCACCCCAGT TTGGTGTAAC TTTGCTGACT TACGAATTGC TACAGCGATG GTTCTACATT
GATTTTGGAG GAGTAAAACC CATGGGATCA GAGCCAGTTC CTAAATCCAG GATCAACCTG
CCTGCCCCGA ATCCTGATCA CGTTGGGGGC TACAAACTGG CAGTTGCTAC ATTTGCAGGG
ATTGAAAACA AATTTGGACT TTACCTACCT CTCTTCAAGC CATCAGTATC TACCTCAAAG
GCTATTGGTG GAGGCCCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006715894.1
CDS95..2155
Misc Feature(1)176..370
Misc Feature(2)182..370
Misc Feature(3)order(200..202,215..217,224..226,242..247,323..325,329..331,335..337,341..343,347..349,356..358)
Misc Feature(4)1106..1897
Misc Feature(5)1112..1393
Misc Feature(6)1397..1660
Misc Feature(7)1673..1960
Translation

Target ORF information:

RefSeq Version XM_006715831.3
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006715831.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGCCAACAC ATGGCAAAGT AAAGGGGGAA GGAGATTTTC TGCTGCATGT GGCTTTAACC 
AAGAGAGCAG ATCCAGCTGA GCTTAGAACA ATATTTTTGA AGTATGCAAG CATTGAGAAA
AACGGTGAAT TTTTCATGTC CCCCAATGAC TTTGTCACTC GATACTTGAA CATTTTTGGA
GAAAGCCAGC CTAATCCAAA GACTGTGGAA CTTTTAAGTG GAGTGGTGGA TCAGACCAAA
GATGGATTAA TATCTTTTCA AGAATTTGTT GCCTTTGAAT CTGTCCTGTG TGCCCCTGAT
GCTTTGTTTA TGGTAGCCTT TCAGCTGTTT GACAAAGCTG GCAAAGGAGA AGTAACTTTT
GAGGATGTTA AGCAAGTTTT TGGACAGACC ACAATTCATC AACATATTCC ATTTAACTGG
GATTCAGAAT TTGTGCAACT ACATTTTGGA AAAGAAAGAA AAAGACACCT GACATATGCG
GAATTTACTC AGTTTTTATT GGAAATACAA CTGGAGCACG CAAAGCAAGC CTTTGTGCAA
CGGGACAATG CTAGGACTGG GAGAGTCACA GCCATCGACT TCCGAGACAT CATGGTCACC
ATCCGCCCCC ATGTCTTGAC TCCTTTTGTA GAAGAATGTC TAGTAGCTGC TGCTGGAGGT
ACCACATCCC ATCAAGTTAG TTTCTCCTAT TTTAATGGAT TTAATTCGCT CCTTAACAAC
ATGGAACTCA TTAGAAAGAT CTATAGCACT CTGGCTGGCA CCAGGAAAGA TGTTGAAGTG
ACTAAGGAGG AGTTTGTTCT GGCAGCTCAG AAATTTGGTC AGGTTACACC CATGGAAGTT
GACATCTTGT TTCAGTTAGC AGATTTATAT GAGCCAAGGG GACGTATGAC CTTAGCAGAC
ATTGAACGGA TTGCTCCTCT GGAAGAGGGA ACTCTGCCCT TTAACTTGGC TGAGGCCCAG
AGGCAGAAGG CCTCAGGTGA TTCAGCTCGA CCAGTTCTTC TACAAGTTGC AGAGTCGGCC
TACAGGTTTG GTCTGGGTTC TGTTGCTGGA GCTGTTGGAG CCACTGCTGT GTATCCTATC
GATCTTGTAA AAACTCGAAT GCAGAACCAA CGATCAACTG GCTCTTTTGT GGGAGAACTC
ATGTATAAAA ACAGCTTTGA CTGTTTTAAG AAAGTGCTAC GCTATGAAGG CTTCTTTGGA
CTGTATAGAG GTCTGTTGCC ACAGTTATTG GGAGTTGCCC CAGAGAAGGC CATAAAACTT
ACAGTGAACG ATTTTGTGAG GGATAAATTT ATGCACAAAG ATGGTTCGGT CCCACTTGCA
GCAGAAATTC TTGCTGGAGG CTGCGCTGGA GGCTCCCAGG TGATTTTCAC AAATCCTTTA
GAAATCGTCA AGATCCGTTT GCAAGTGGCA GGAGAAATCA CCACTGGTCC TCGAGTCAGT
GCTCTGTCTG TCGTGCGGGA CCTGGGGTTT TTTGGGATCT ACAAGGGTGC CAAAGCATGC
TTTCTGCGGG ACATTCCTTT CTCGGCCATC TACTTTCCGT GCTATGCTCA TGTGAAGGCT
TCCTTTGCAA ATGAAGATGG GCAGGTTAGC CCAGGAAGCC TGCTCTTAGC TGGTGCCATA
GCTGGTATGC CTGCAGCATC TTTAGTGACC CCTGCTGATG TTATCAAGAC GAGATTACAG
GTGGCTGCCC GGGCTGGCCA AACCACTTAC AGCGGAGTGA TAGACTGCTT TAGAAAGATA
CTGCGTGAAG AAGGACCAAA AGCTCTGTGG AAGGGAGCTG GTGCTCGTGT ATTTCGATCC
TCACCCCAGT TTGGTGTAAC TTTGCTGACT TACGAATTGC TACAGCGATG GTTCTACATT
GATTTTGGAG GAGTAAAACC CATGGGATCA GAGCCAGTTC CTAAATCCAG GATCAACCTG
CCTGCCCCGA ATCCTGATCA CGTTGGGGGC TACAAACTGG CAGTTGCTAC ATTTGCAGGG
ATTGAAAACA AATTTGGACT TTACCTACCT CTCTTCAAGC CATCAGTATC TACCTCAAAG
GCTATTGGTG GAGGCCCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97000
Clone ID Related Accession (Same CDS sequence) XM_017011663.1
Accession Version XM_017011663.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product calcium-binding mitochondrial carrier protein Aralar2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGATTCAGT TATCTCCACC TGGCCCCGCC CTGGACACAT GGGGATTATT ACAATTCAAG 
TATGCAAGCA TTGAGAAAAA CGGTGAATTT TTCATGTCCC CCAATGACTT TGTCACTCGA
TACTTGAACA TTTTTGGAGA AAGCCAGCCT AATCCAAAGA CTGTGGAACT TTTAAGTGGA
GTGGTGGATC AGACCAAAGA TGGATTAATA TCTTTTCAAG AATTTGTTGC CTTTGAATCT
GTCCTGTGTG CCCCTGATGC TTTGTTTATG GTAGCCTTTC AGCTGTTTGA CAAAGCTGGC
AAAGGAGAAG TAACTTTTGA GGATGTTAAG CAAGTTTTTG GACAGACCAC AATTCATCAA
CATATTCCAT TTAACTGGGA TTCAGAATTT GTGCAACTAC ATTTTGGAAA AGAAAGAAAA
AGACACCTGA CATATGCGGA ATTTACTCAG TTTTTATTGG AAATACAACT GGAGCACGCA
AAGCAAGCCT TTGTGCAACG GGACAATGCT AGGACTGGGA GAGTCACAGC CATCGACTTC
CGAGACATCA TGGTCACCAT CCGCCCCCAT GTCTTGACTC CTTTTGTAGA AGAATGTCTA
GTAGCTGCTG CTGGAGGTAC CACATCCCAT CAAGTTAGTT TCTCCTATTT TAATGGATTT
AATTCGCTCC TTAACAACAT GGAACTCATT AGAAAGATCT ATAGCACTCT GGCTGGCACC
AGGAAAGATG TTGAAGTGAC TAAGGAGGAG TTTGTTCTGG CAGCTCAGAA ATTTGGTCAG
GTTACACCCA TGGAAGTTGA CATCTTGTTT CAGTTAGCAG ATTTATATGA GCCAAGGGGA
CGTATGACCT TAGCAGACAT TGAACGGATT GCTCCTCTGG AAGAGGGAAC TCTGCCCTTT
AACTTGGCTG AGGCCCAGAG GCAGAAGGCC TCAGGTGATT CAGCTCGACC AGTTCTTCTA
CAAGTTGCAG AGTCGGCCTA CAGGTTTGGT CTGGGTTCTG TTGCTGGAGC TGTTGGAGCC
ACTGCTGTGT ATCCTATCGA TCTTGTAAAA ACTCGAATGC AGAACCAACG ATCAACTGGC
TCTTTTGTGG GAGAACTCAT GTATAAAAAC AGCTTTGACT GTTTTAAGAA AGTGCTACGC
TATGAAGGCT TCTTTGGACT GTATAGAGGT CTGTTGCCAC AGTTATTGGG AGTTGCCCCA
GAGAAGGCCA TAAAACTTAC AGTGAACGAT TTTGTGAGGG ATAAATTTAT GCACAAAGAT
GGTTCGGTCC CACTTGCAGC AGAAATTCTT GCTGGAGGCT GCGCTGGAGG CTCCCAGGTG
ATTTTCACAA ATCCTTTAGA AATCGTCAAG ATCCGTTTGC AAGTGGCAGG AGAAATCACC
ACTGGTCCTC GAGTCAGTGC TCTGTCTGTC GTGCGGGACC TGGGGTTTTT TGGGATCTAC
AAGGGTGCCA AAGCATGCTT TCTGCGGGAC ATTCCTTTCT CGGCCATCTA CTTTCCGTGC
TATGCTCATG TGAAGGCTTC CTTTGCAAAT GAAGATGGGC AGGTTAGCCC AGGAAGCCTG
CTCTTAGCTG GTGCCATAGC TGGTATGCCT GCAGCATCTT TAGTGACCCC TGCTGATGTT
ATCAAGACGA GATTACAGGT GGCTGCCCGG GCTGGCCAAA CCACTTACAG CGGAGTGATA
GACTGCTTTA GAAAGATACT GCGTGAAGAA GGACCAAAAG CTCTGTGGAA GGGAGCTGGT
GCTCGTGTAT TTCGATCCTC ACCCCAGTTT GGTGTAACTT TGCTGACTTA CGAATTGCTA
CAGCGATGGT TCTACATTGA TTTTGGAGGA GTAAAACCCA TGGGATCAGA GCCAGTTCCT
AAATCCAGGA TCAACCTGCC TGCCCCGAAT CCTGATCACG TTGGGGGCTA CAAACTGGCA
GTTGCTACAT TTGCAGGGAT TGAAAACAAA TTTGGACTTT ACCTACCTCT CTTCAAGCCA
TCAGTATCTA CCTCAAAGGC TATTGGTGGA GGCCCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867152.1
CDS52..2070
Translation

Target ORF information:

RefSeq Version XM_017011663.1
Organism Homo sapiens(Human)
Definition Homo sapiens solute carrier family 25 member 13 (SLC25A13), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017011663.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGATTCAGT TATCTCCACC TGGCCCCGCC CTGGACACAT GGGGATTATT ACAATTCAAG 
TATGCAAGCA TTGAGAAAAA CGGTGAATTT TTCATGTCCC CCAATGACTT TGTCACTCGA
TACTTGAACA TTTTTGGAGA AAGCCAGCCT AATCCAAAGA CTGTGGAACT TTTAAGTGGA
GTGGTGGATC AGACCAAAGA TGGATTAATA TCTTTTCAAG AATTTGTTGC CTTTGAATCT
GTCCTGTGTG CCCCTGATGC TTTGTTTATG GTAGCCTTTC AGCTGTTTGA CAAAGCTGGC
AAAGGAGAAG TAACTTTTGA GGATGTTAAG CAAGTTTTTG GACAGACCAC AATTCATCAA
CATATTCCAT TTAACTGGGA TTCAGAATTT GTGCAACTAC ATTTTGGAAA AGAAAGAAAA
AGACACCTGA CATATGCGGA ATTTACTCAG TTTTTATTGG AAATACAACT GGAGCACGCA
AAGCAAGCCT TTGTGCAACG GGACAATGCT AGGACTGGGA GAGTCACAGC CATCGACTTC
CGAGACATCA TGGTCACCAT CCGCCCCCAT GTCTTGACTC CTTTTGTAGA AGAATGTCTA
GTAGCTGCTG CTGGAGGTAC CACATCCCAT CAAGTTAGTT TCTCCTATTT TAATGGATTT
AATTCGCTCC TTAACAACAT GGAACTCATT AGAAAGATCT ATAGCACTCT GGCTGGCACC
AGGAAAGATG TTGAAGTGAC TAAGGAGGAG TTTGTTCTGG CAGCTCAGAA ATTTGGTCAG
GTTACACCCA TGGAAGTTGA CATCTTGTTT CAGTTAGCAG ATTTATATGA GCCAAGGGGA
CGTATGACCT TAGCAGACAT TGAACGGATT GCTCCTCTGG AAGAGGGAAC TCTGCCCTTT
AACTTGGCTG AGGCCCAGAG GCAGAAGGCC TCAGGTGATT CAGCTCGACC AGTTCTTCTA
CAAGTTGCAG AGTCGGCCTA CAGGTTTGGT CTGGGTTCTG TTGCTGGAGC TGTTGGAGCC
ACTGCTGTGT ATCCTATCGA TCTTGTAAAA ACTCGAATGC AGAACCAACG ATCAACTGGC
TCTTTTGTGG GAGAACTCAT GTATAAAAAC AGCTTTGACT GTTTTAAGAA AGTGCTACGC
TATGAAGGCT TCTTTGGACT GTATAGAGGT CTGTTGCCAC AGTTATTGGG AGTTGCCCCA
GAGAAGGCCA TAAAACTTAC AGTGAACGAT TTTGTGAGGG ATAAATTTAT GCACAAAGAT
GGTTCGGTCC CACTTGCAGC AGAAATTCTT GCTGGAGGCT GCGCTGGAGG CTCCCAGGTG
ATTTTCACAA ATCCTTTAGA AATCGTCAAG ATCCGTTTGC AAGTGGCAGG AGAAATCACC
ACTGGTCCTC GAGTCAGTGC TCTGTCTGTC GTGCGGGACC TGGGGTTTTT TGGGATCTAC
AAGGGTGCCA AAGCATGCTT TCTGCGGGAC ATTCCTTTCT CGGCCATCTA CTTTCCGTGC
TATGCTCATG TGAAGGCTTC CTTTGCAAAT GAAGATGGGC AGGTTAGCCC AGGAAGCCTG
CTCTTAGCTG GTGCCATAGC TGGTATGCCT GCAGCATCTT TAGTGACCCC TGCTGATGTT
ATCAAGACGA GATTACAGGT GGCTGCCCGG GCTGGCCAAA CCACTTACAG CGGAGTGATA
GACTGCTTTA GAAAGATACT GCGTGAAGAA GGACCAAAAG CTCTGTGGAA GGGAGCTGGT
GCTCGTGTAT TTCGATCCTC ACCCCAGTTT GGTGTAACTT TGCTGACTTA CGAATTGCTA
CAGCGATGGT TCTACATTGA TTTTGGAGGA GTAAAACCCA TGGGATCAGA GCCAGTTCCT
AAATCCAGGA TCAACCTGCC TGCCCCGAAT CCTGATCACG TTGGGGGCTA CAAACTGGCA
GTTGCTACAT TTGCAGGGAT TGAAAACAAA TTTGGACTTT ACCTACCTCT CTTCAAGCCA
TCAGTATCTA CCTCAAAGGC TATTGGTGGA GGCCCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

SLC25A13 cDNA cloning analysis using peripheral blood lymphocytes facilitates the identification of a large deletion mutation: Molecular diagnosis of an infant with neonatal intrahepatic cholestasis caused by citrin deficiency.
Molecular medicine reports14(6)5189-5194(2016 Dec)
Zeng HS,Lin WX,Zhao ST,Zhang ZH,Yang HW,Chen FP,Song YZ,Yin ZN


book

[Analysis of clinical features and SLC25A13 gene mutations in a family affected with neonatal intrahepatic cholestasis].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics33(5)670-3(2016 Oct)
Wang L,Cheng X,Yan L,Wei Y,Tang F,Dong X,Yuan Y,Xie Y


book

Identification of a Large SLC25A13 Deletion via Sophisticated Molecular Analyses Using Peripheral Blood Lymphocytes in an Infant with Neonatal Intrahepatic Cholestasis Caused by Citrin Deficiency (NICCD): A Clinical and Molecular Study.
BioMed research international20164124263(2016)
Zheng QQ,Zhang ZH,Zeng HS,Lin WX,Yang HW,Yin ZN,Song YZ


book

Calcium-induced conformational changes of the regulatory domain of human mitochondrial aspartate/glutamate carriers.
Nature communications55491(2014 Nov)
Thangaratnarajah C,Ruprecht JJ,Kunji ER


book

First Bulgarian case of citrin deficiency caused by one novel and one recurrent mutation in the SLC25A13 gene.
Genetic counseling (Geneva, Switzerland)25(3)271-6(2014)
Avdjieva-Tzavella DM,Ivanova MB,Todorov TP,Todorova AP,Panteleeva EI,Tincheva SS,Lazarova EA,Kathom HM,Yaneva PG,Tincheva RS


 
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