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SLC25A15 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SLC25A15
Entrez Gene ID 10166
Full Name solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
Synonyms D13S327, HHH, ORC1, ORNT1
General protein information
Preferred Names
mitochondrial ornithine transporter 1
Names
mitochondrial ornithine transporter 1
ornithine transporter 1
solute carrier family 25 member 15
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q14

Summary This gene is a member of the mitochondrial carrier family. The encoded protein transports ornithine across the inner mitochondrial membrane from the cytosol to the mitochondrial matrix. The protein is an essential component of the urea cycle, and functions in ammonium detoxification and biosynthesis of the amino acid arginine. Mutations in this gene result in hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome. There is a pseudogene of this locus on the Y chromosome.[provided by RefSeq, May 2009]. lac of sum
Disorder MIM:

603861

Disorder Html: Hyperornithinemia-hyperammonemia-homocitrullinemia syndrome, 238970

mRNA and Protein(s)

mRNA Protein Name
NM_014252 NP_055067 mitochondrial ornithine transporter 1



Homo sapiens (human) SLC25A15 NP_055067.1
Pan troglodytes (chimpanzee) SLC25A15 XP_003954299.1
Macaca mulatta (Rhesus monkey) SLC25A15 XP_001088596.1
Canis lupus familiaris (dog) SLC25A15 XP_543118.1
Bos taurus (cattle) SLC25A15 NP_001039791.1
Mus musculus (house mouse) Slc25a15 NP_851842.1
Rattus norvegicus (Norway rat) Slc25a15 NP_001041345.1
Gallus gallus (chicken) SLC25A15 NP_001008442.1
Danio rerio (zebrafish) slc25a15a NP_001074107.1
Danio rerio (zebrafish) LOC565335 NP_001121816.1
Drosophila melanogaster (fruit fly) CG1628 NP_001259423.1
Caenorhabditis elegans T10F2.2 NP_498094.1
Xenopus (Silurana) tropicalis (western clawed frog) slc25a15 NP_001090866.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC25A15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC25A15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22381 NM_014252 Homo sapiens solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15 (SLC25A15), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22381
Clone ID Related Accession (Same CDS sequence) NM_014252
Accession Version NM_014252.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 906bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitochondrial ornithine transporter 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB505424.1, BI461478.1, BC002702.2, BU726339.1, AL161614.16 and AA948095.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2009 this sequence version replaced gi:88703039. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AF112968.1, AF177333.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAATCCA ATCCTGCTAT CCAGGCTGCC ATTGACCTCA CAGCGGGGGC TGCAGGAGGT 
ACAGCATGTG TACTGACCGG GCAGCCCTTT GACACAATGA AAGTGAAGAT GCAGACGTTC
CCTGACCTGT ACCGGGGCCT CACCGACTGC TGCCTGAAGA CTTACTCCCA GGTGGGCTTC
CGTGGCTTCT ACAAGGGTAC CAGTCCAGCA CTAATCGCCA ACATCGCTGA GAACTCAGTC
CTCTTCATGT GCTACGGCTT CTGCCAGCAG GTGGTGCGGA AAGTGGCTGG ATTGGACAAG
CAGGCAAAGC TGAGTGATCT GCAGAATGCA GCCGCCGGTT CCTTCGCCTC TGCCTTTGCT
GCACTGGTGC TCTGCCCCAC GGAGCTCGTG AAGTGCCGGC TGCAGACCAT GTATGAGATG
GAGACATCAG GGAAGATAGC CAAGAGCCAG AATACAGTGT GGTCTGTCAT CAAAAGTATT
CTTAGGAAAG ATGGCCCCTT GGGGTTCTAC CATGGACTCT CAAGCACTTT ACTTCGAGAA
GTACCAGGCT ATTTCTTCTT CTTCGGTGGC TATGAACTGA GCCGGTCCTT TTTTGCATCA
GGGAGATCAA AAGATGAATT AGGCCCTGTA CCTTTGATGT TAAGTGGTGG AGTTGGTGGG
ATTTGCCTCT GGCTTGCGGT ATACCCAGTG GATTGTATCA AATCCAGAAT TCAAGTTCTT
TCCATGTCTG GAAAACAGGC AGGATTTATC AGAACCTTTA TAAATGTTGT GAAAAATGAA
GGAATAACGG CCTTATATTC TGGACTGAAA CCTACTATGA TTCGAGCATT CCCTGCCAAT
GGAGCACTCT TTTTGGCCTA CGAATATAGC AGGAAGTTGA TGATGAACCA GTTGGAAGCA
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055067.1
CDS323..1228
Misc Feature(1)224..226(+)
Misc Feature(2)335..397(+)
Misc Feature(3)341..595(+)
Misc Feature(4)356..604(+)
Misc Feature(5)524..586(+)
Misc Feature(6)632..913(+)
Misc Feature(7)650..712(+)
Misc Feature(8)686..916(+)
Misc Feature(9)824..886(+)
Misc Feature(10)935..1210(+)
Misc Feature(11)941..1201(+)
Misc Feature(12)941..1003(+)
Misc Feature(13)1031..1093(+)
Exon (1)1..253
Gene:SLC25A15
Gene Synonym:
Exon (2)254..377
Gene:SLC25A15
Gene Synonym:
Exon (3)378..636
Gene:SLC25A15
Gene Synonym:
Exon (4)637..774
Gene:SLC25A15
Gene Synonym:
Exon (5)775..944
Gene:SLC25A15
Gene Synonym:
Exon (6)945..1103
Gene:SLC25A15
Gene Synonym:
Exon (7)1104..4021
Gene:SLC25A15
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014252
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15 (SLC25A15), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014252

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAATCCA ATCCTGCTAT CCAGGCTGCC ATTGACCTCA CAGCGGGGGC TGCAGGAGGT 
ACAGCATGTG TACTGACCGG GCAGCCCTTT GACACAATGA AAGTGAAGAT GCAGACGTTC
CCTGACCTGT ACCGGGGCCT CACCGACTGC TGCCTGAAGA CTTACTCCCA GGTGGGCTTC
CGTGGCTTCT ACAAGGGTAC CAGTCCAGCA CTAATCGCCA ACATCGCTGA GAACTCAGTC
CTCTTCATGT GCTACGGCTT CTGCCAGCAG GTGGTGCGGA AAGTGGCTGG ATTGGACAAG
CAGGCAAAGC TGAGTGATCT GCAGAATGCA GCCGCCGGTT CCTTCGCCTC TGCCTTTGCT
GCACTGGTGC TCTGCCCCAC GGAGCTCGTG AAGTGCCGGC TGCAGACCAT GTATGAGATG
GAGACATCAG GGAAGATAGC CAAGAGCCAG AATACAGTGT GGTCTGTCAT CAAAAGTATT
CTTAGGAAAG ATGGCCCCTT GGGGTTCTAC CATGGACTCT CAAGCACTTT ACTTCGAGAA
GTACCAGGCT ATTTCTTCTT CTTCGGTGGC TATGAACTGA GCCGGTCCTT TTTTGCATCA
GGGAGATCAA AAGATGAATT AGGCCCTGTA CCTTTGATGT TAAGTGGTGG AGTTGGTGGG
ATTTGCCTCT GGCTTGCGGT ATACCCAGTG GATTGTATCA AATCCAGAAT TCAAGTTCTT
TCCATGTCTG GAAAACAGGC AGGATTTATC AGAACCTTTA TAAATGTTGT GAAAAATGAA
GGAATAACGG CCTTATATTC TGGACTGAAA CCTACTATGA TTCGAGCATT CCCTGCCAAT
GGAGCACTCT TTTTGGCCTA CGAATATAGC AGGAAGTTGA TGATGAACCA GTTGGAAGCA
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A genome-wide association study identifies 2 susceptibility Loci for Crohn's disease in a Japanese population
Gastroenterology 144 (4), 781-788 (2013)
Yamazaki K, Umeno J, Takahashi A, Hirano A, Johnson TA, Kumasaka N, Morizono T, Hosono N, Kawaguchi T, Takazoe M, Yamada T, Suzuki Y, Tanaka H, Motoya S, Hosokawa M, Arimura Y, Shinomura Y, Matsui T, Matsumoto T, Iida M, Tsunoda T, Nakamura Y, Kamatani N and Kubo M.


book

The mitochondrial transporter family SLC25: identification, properties and physiopathology
Mol. Aspects Med. 34 (2-3), 465-484 (2013)
Palmieri F.


book

Long-term follow-up of four patients affected by HHH syndrome
Clin. Chim. Acta 413 (13-14), 1151-1155 (2012)
Kim SZ, Song WJ, Nyhan WL, Ficicioglu C, Mandell R and Shih VE.


book

Substrate specificity of the two mitochondrial ornithine carriers can be swapped by single mutation in substrate binding site
J. Biol. Chem. 287 (11), 7925-7934 (2012)
Monne M, Miniero DV, Daddabbo L, Robinson AJ, Kunji ER and Palmieri F.


book

Insights into the mutation-induced HHH syndrome from modeling human mitochondrial ornithine transporter-1
PLoS ONE 7 (1), E31048 (2012)
Wang JF and Chou KC.


book

Clinical and molecular findings in hyperornithinemia-hyperammonemia-homocitrullinuria syndrome
Neurology 57 (5), 911-914 (2001)
Salvi S, Santorelli FM, Bertini E, Boldrini R, Meli C, Donati A, Burlina AB, Rizzo C, Di Capua M, Fariello G and Dionisi-Vici C.


book

Diagnosis of Japanese patients with HHH syndrome by molecular genetic analysis: a common mutation, R179X
J. Hum. Genet. 46 (5), 260-262 (2001)
Miyamoto T, Kanazawa N, Kato S, Kawakami M, Inoue Y, Kuhara T, Inoue T, Takeshita K and Tsujino S.


book

Three novel mutations (G27E, insAAC, R179X) in the ORNT1 gene of Japanese patients with hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome
Ann. Neurol. 47 (5), 625-631 (2000)
Tsujino S, Kanazawa N, Ohashi T, Eto Y, Saito T, Kira J and Yamada T.


book

Hyperornithinaemia-hyperammonaemia-homocitrullinuria syndrome is caused by mutations in a gene encoding a mitochondrial ornithine transporter
Nat. Genet. 22 (2), 151-158 (1999)
Camacho JA, Obie C, Biery B, Goodman BK, Hu CA, Almashanu S, Steel G, Casey R, Lambert M, Mitchell GA and Valle D.


book

Hyperornithinemia-Hyperammonemia-Homocitrullinuria Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Camacho,J. and Rioseco-Camacho,N.


 
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