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CDKN2B cDNA ORF clone, Homo sapiens (human)

Gene Symbol CDKN2B
Entrez Gene ID 1030
Full Name cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Synonyms CDK4I, INK4B, MTS2, P15, TP15, p15INK4b
General protein information
Preferred Names
cyclin-dependent kinase 4 inhibitor B
Names
cyclin-dependent kinase 4 inhibitor B
MTS-2
p14-INK4b
p14_INK4B
p15-INK4b
p15_INK4B
CDK4B inhibitor
p14_CDK inhibitor
p15 CDK inhibitor
CDK inhibitory protein
multiple tumor suppressor 2
cyclin-dependent kinases 4 and 6 binding protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9p21

Summary This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600431


mRNA and Protein(s)

mRNA Protein Name
NM_004936 NP_004927 cyclin-dependent kinase 4 inhibitor B isoform 1
NM_078487 NP_511042 cyclin-dependent kinase 4 inhibitor B isoform 2



Homo sapiens (human) CDKN2B NP_004927.2
Pan troglodytes (chimpanzee) CDKN2B XP_003312069.1
Macaca mulatta (Rhesus monkey) CDKN2B XP_001107263.1
Canis lupus familiaris (dog) CDKN2B NP_001139741.1
Bos taurus (cattle) CDKN2B NP_001069362.1
Mus musculus (house mouse) Cdkn2b NP_031696.1
Rattus norvegicus (Norway rat) Cdkn2b NP_570825.1
Gallus gallus (chicken) CDKN2B NP_989764.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CDKN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu10080
NM_004936 Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu18518 NM_078487 Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu10080
Clone ID Related Accession (Same CDS sequence) NM_004936
Accession Version NM_004936.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 417bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cyclin-dependent kinase 4 inhibitor B isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI560960.1, BC014469.1, BC018984.2 and AF488731.1. On May 12, 2004 this sequence version replaced gi:17981693. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014469.1, AL555679.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGCGCGAGG AGAACAAGGG CATGCCCAGT GGGGGCGGCA GCGATGAGGG TCTGGCCAGC 
GCCGCGGCGC GGGGACTAGT GGAGAAGGTG CGACAGCTCC TGGAAGCCGG CGCGGATCCC
AACGGAGTCA ACCGTTTCGG GAGGCGCGCG ATCCAGGTCA TGATGATGGG CAGCGCCCGC
GTGGCGGAGC TGCTGCTGCT CCACGGCGCG GAGCCCAACT GCGCAGACCC TGCCACTCTC
ACCCGACCGG TGCATGATGC TGCCCGGGAG GGCTTCCTGG ACACGCTGGT GGTGCTGCAC
CGGGCCGGGG CGCGGCTGGA CGTGCGCGAT GCCTGGGGTC GTCTGCCCGT GGACTTGGCC
GAGGAGCGGG GCCACCGCGA CGTTGCAGGG TACCTGCGCA CAGCCACGGG GGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004927.2
CDS361..777
Misc Feature(1)34..36(+)
Misc Feature(2)397..477(+)
Misc Feature(3)412..690(+)
Misc Feature(4)421..756(+)
Misc Feature(5)496..582(+)
Misc Feature(6)595..684(+)
Misc Feature(7)694..774(+)
Exon (1)1..516
Gene:CDKN2B
Gene Synonym:
Exon (2)517..3861
Gene:CDKN2B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004936
Organism Homo sapiens (human)
Definition Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004936

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCGCGAGG AGAACAAGGG CATGCCCAGT GGGGGCGGCA GCGATGAGGG TCTGGCCAGC 
GCCGCGGCGC GGGGACTAGT GGAGAAGGTG CGACAGCTCC TGGAAGCCGG CGCGGATCCC
AACGGAGTCA ACCGTTTCGG GAGGCGCGCG ATCCAGGTCA TGATGATGGG CAGCGCCCGC
GTGGCGGAGC TGCTGCTGCT CCACGGCGCG GAGCCCAACT GCGCAGACCC TGCCACTCTC
ACCCGACCGG TGCATGATGC TGCCCGGGAG GGCTTCCTGG ACACGCTGGT GGTGCTGCAC
CGGGCCGGGG CGCGGCTGGA CGTGCGCGAT GCCTGGGGTC GTCTGCCCGT GGACTTGGCC
GAGGAGCGGG GCCACCGCGA CGTTGCAGGG TACCTGCGCA CAGCCACGGG GGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18518
Clone ID Related Accession (Same CDS sequence) NM_078487
Accession Version NM_078487.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cyclin-dependent kinase 4 inhibitor B isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI560960.1, AF004819.1, BC014469.1, BC018984.2 and AF488731.1. This sequence is a reference standard in the RefSeqGene project. On May 12, 2004 this sequence version replaced gi:17981695. Transcript Variant: This variant (2) uses a different splice site, which leads to a translation frame shift, when compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF004819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152568, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGCGCGAGG AGAACAAGGG CATGCCCAGT GGGGGCGGCA GCGATGAGGG TCTGGCCAGC 
GCCGCGGCGC GGGGACTAGT GGAGAAGGTG CGACAGCTCC TGGAAGCCGG CGCGGATCCC
AACGGAGTCA ACCGTTTCGG GAGGCGCGCG ATCCAGGTAG CTGGGGCCCC AGGGCCTCGC
CGGCAGGGGG CGCGCGAACG CGGGGCGCGG CCTCGGCGGA TCGGGGCTGG AACCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_511042.1
CDS361..597
Misc Feature(1)34..36(+)
Misc Feature(2)397..477(+)
Misc Feature(3)412..>522(+)
Misc Feature(4)421..>522(+)
Misc Feature(5)517..639(+)
Exon (1)1..639
Gene:CDKN2B
Gene Synonym:
Exon (2)640..3984
Gene:CDKN2B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_078487
Organism Homo sapiens (human)
Definition Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_078487

ORF Insert Sequence:

1
61
121
181
ATGCGCGAGG AGAACAAGGG CATGCCCAGT GGGGGCGGCA GCGATGAGGG TCTGGCCAGC 
GCCGCGGCGC GGGGACTAGT GGAGAAGGTG CGACAGCTCC TGGAAGCCGG CGCGGATCCC
AACGGAGTCA ACCGTTTCGG GAGGCGCGCG ATCCAGGTAG CTGGGGCCCC AGGGCCTCGC
CGGCAGGGGG CGCGCGAACG CGGGGCGCGG CCTCGGCGGA TCGGGGCTGG AACCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide association study identifies three novel loci for type 2 diabetes
Hum. Mol. Genet. 23 (1), 239-246 (2014)
Hara K, Fujita H, Johnson TA, Yamauchi T, Yasuda K, Horikoshi M, Peng C, Hu C, Ma RC, Imamura M, Iwata M, Tsunoda T, Morizono T, Shojima N, So WY, Leung TF, Kwan P, Zhang R, Wang J, Yu W, Maegawa H, Hirose H, Kaku K, Ito C, Watada H, Tanaka Y, Tobe K, Kashiwagi A, Kawamori R, Jia W, Chan JC, Teo YY, Shyong TE, Kamatani N, Kubo M, Maeda S and Kadowaki T.


book

The relationship between five widely-evaluated variants in CDKN2A/B and CDKAL1 genes and the risk of type 2 diabetes: a meta-analysis
Gene 531 (2), 435-443 (2013)
Peng F, Hu D, Gu C, Li X, Li Y, Jia N, Chu S, Lin J and Niu W.


book

Genome-wide association study identifies multiple risk loci for chronic lymphocytic leukemia
Nat. Genet. 45 (8), 868-876 (2013)
Berndt SI, Skibola CF, Joseph V, Camp NJ, Nieters A, Wang Z, Cozen W, Monnereau A, Wang SS, Kelly RS, Lan Q, Teras LR, Chatterjee N, Chung CC, Yeager M, Brooks-Wilson AR, Hartge P, Purdue MP, Birmann BM, Armstrong BK, Cocco P, Zhang Y, Severi G, Zeleniuch-Jacquotte A, Lawrence C, Burdette L, Yuenger J, Hutchinson A, Jacobs KB, Call TG, Shanafelt TD, Novak AJ, Kay NE, Liebow M, Wang AH, Smedby KE, Adami HO, Melbye M, Glimelius B, Chang ET, Glenn M, Curtin K, Cannon-Albright LA, Jones B, Diver WR, Link BK, Weiner GJ, Conde L, Bracci PM, Riby J, Holly EA, Smith MT, Jackson RD, Tinker LF, Benavente Y, Becker N, Boffetta P, Brennan P, Foretova L, Maynadie M, McKay J, Staines A, Rabe KG, Achenbach SJ, Vachon CM, Goldin LR, Strom SS, Lanasa MC, Spector LG, Leis JF, Cunningham JM, Weinberg JB, Morrison VA, Caporaso NE, Norman AD, Linet MS, De Roos AJ, Morton LM, Severson RK, Riboli E, Vineis P, Kaaks R, Trichopoulos D, Masala G, Weiderpass E, Chirlaque MD, Vermeulen RC, Travis RC, Giles GG, Albanes D, Virtamo J, Weinstein S, Clavel J, Zheng T, Holford TR, Offit K, Zelenetz A, Klein RJ, Spinelli JJ, Bertrand KA, Laden F, Giovannucci E, Kraft P, Kricker A, Turner J, Vajdic CM, Ennas MG, Ferri GM, Miligi L, Liang L, Sampson J, Crouch S, Park JH, North KE, Cox A, Snowden JA, Wright J, Carracedo A, Lopez-Otin C, Bea S, Salaverria I, Martin-Garcia D, Campo E, Fraumeni JF Jr, de Sanjose S, Hjalgrim H, Cerhan JR, Chanock SJ, Rothman N and Slager SL.


book

Expression of p15INK(4)b and p57KIP(2) and relationship with clinicopathological features and prognosis in patients with vulvar squamous cell carcinoma
PLoS ONE 8 (4), E61273 (2013)
Holm R, Forsund M, Nguyen MT, Nesland JM and Trope CG.


book

Quantitative comparison of CDKN2B methylation in pediatric and adult myelodysplastic syndromes
Acta Haematol. 130 (2), 115-121 (2013)
Kim M, Kook H, Park HJ, Ahn HS, Lee KC, Lee KS, Park SK, Lim JY, Kim HK, Han DK and Lee DS.


book

Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins
Oncogene 11 (8), 1581-1588 (1995)
Hall M, Bates S and Peters G.


book

Genomic structure, expression and mutational analysis of the P15 (MTS2) gene
Oncogene 11 (5), 987-991 (1995)
Stone S, Dayananth P, Jiang P, Weaver-Feldhaus JM, Tavtigian SV, Cannon-Albright L and Kamb A.


book

Mutations in the p16INK4/MTS1/CDKN2, p15INK4B/MTS2, and p18 genes in primary and metastatic lung cancer
Cancer Res. 55 (7), 1448-1451 (1995)
Okamoto A, Hussain SP, Hagiwara K, Spillare EA, Rusin MR, Demetrick DJ, Serrano M, Hannon GJ, Shiseki M and Zariwala M.


book

Growth suppression by p18, a p16INK4/MTS1- and p14INK4B/MTS2-related CDK6 inhibitor, correlates with wild-type pRb function
Genes Dev. 8 (24), 2939-2952 (1994)
Guan KL, Jenkins CW, Li Y, Nichols MA, Wu X, O'Keefe CL, Matera AG and Xiong Y.


book

Deletion of p16 and p15 genes in brain tumors
Cancer Res. 54 (24), 6353-6358 (1994)
Jen J, Harper JW, Bigner SH, Bigner DD, Papadopoulos N, Markowitz S, Willson JK, Kinzler KW and Vogelstein B.


 
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