PRMT5 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following PRMT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRMT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23684 NM_006109.5
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu27546 NM_001282955.2
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu27484 NM_001282954.2
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu27659 NM_001039619.3
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu27484 XM_017020927.1
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu23914 NM_001282956.2
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu23924 NM_001282953.2
Latest version!
Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu27546 NM_001282955.1 Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu27484 NM_001282954.1 Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu23924 NM_001282953.1 Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu27659 NM_001039619.2 Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu23684 NM_006109.4 Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu23914 NM_001282956.1 Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23684
    Clone ID Related Accession (Same CDS sequence) NM_006109.5 , NM_006109.4
    Accession Version NM_006109.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1 and AL833612.1. On Nov 23, 2018 this sequence version replaced NM_006109.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.938793.1, SRR1660805.124747.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000324366.13/ ENSP00000319169.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG
    CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATTCGC
    AGGAACTCCG AGGCGGCCAT GTTACAGGAG CTGAATTTTG GTGCATATTT GGGTCTTCCA
    GCTTTCCTGC TGCCCCTTAA TCAGGAAGAT AACACCAACC TGGCCAGAGT TTTGACCAAC
    CACATCCACA CTGGCCATCA CTCTTCCATG TTCTGGATGC GGGTACCCTT GGTGGCACCA
    GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAACTA CACACACAGA GGAGTACAGT
    GGGGAGGAGA AAACGTGGAT GTGGTGGCAC AACTTCCGGA CTTTGTGTGA CTATAGTAAG
    AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGC
    TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACTA GCATTTTCCT GACCAATAAG
    AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTCA TCTTCCGGCT CCTCAAGTTG
    GAGGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCCTAC
    CTCCAATACC TGGAATACTT AAGCCAGAAC CGTCCTCCAC CTAATGCCTA TGAACTCTTT
    GCCAAGGGCT ATGAAGACTA TCTGCAGTCC CCGCTTCAGC CACTGATGGA CAATCTGGAA
    TCTCAGACAT ATGAAGTGTT TGAAAAGGAC CCCATCAAAT ACTCTCAGTA CCAGCAGGCC
    ATCTATAAAT GTCTGCTAGA CCGAGTACCA GAAGAGGAGA AGGATACCAA TGTCCAGGTA
    CTGATGGTGC TGGGAGCAGG ACGGGGACCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG
    CAGGCCGACC GGCGGATAAA GCTGTATGCT GTGGAGAAAA ACCCAAATGC CGTGGTGACG
    CTAGAGAACT GGCAGTTTGA AGAATGGGGA AGCCAAGTGA CCGTAGTCTC ATCAGACATG
    AGGGAATGGG TGGCTCCAGA GAAAGCAGAC ATCATTGTCA GTGAGCTTCT GGGCTCATTT
    GCTGACAATG AATTGTCGCC TGAGTGCCTG GATGGAGCCC AGCACTTCCT AAAAGATGAT
    GGTGTGAGCA TCCCCGGGGA GTACACTTCC TTTCTGGCTC CCATCTCTTC CTCCAAGCTG
    TACAATGAGG TCCGAGCCTG TAGGGAGAAG GACCGTGACC CTGAGGCCCA GTTTGAGATG
    CCTTATGTGG TACGGCTGCA CAACTTCCAC CAGCTCTCTG CACCCCAGCC CTGTTTCACC
    TTCAGCCATC CCAACAGAGA TCCTATGATT GACAACAACC GCTATTGCAC CTTGGAATTT
    CCTGTGGAGG TGAACACAGT ACTACATGGC TTTGCCGGCT ACTTTGAGAC TGTGCTTTAT
    CAGGACATCA CTCTGAGTAT CCGTCCAGAG ACTCACTCTC CTGGGATGTT CTCATGGTTT
    CCCATCCTCT TCCCTATTAA GCAGCCCATA ACGGTACGTG AAGGCCAAAC CATCTGTGTG
    CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA
    GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATATA CCATTGGCCT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006100.2
    CDS16..1929
    Translation

    Target ORF information:

    RefSeq Version NM_006109.5
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006109.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG
    CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATTCGC
    AGGAACTCCG AGGCGGCCAT GTTACAGGAG CTGAATTTTG GTGCATATTT GGGTCTTCCA
    GCTTTCCTGC TGCCCCTTAA TCAGGAAGAT AACACCAACC TGGCCAGAGT TTTGACCAAC
    CACATCCACA CTGGCCATCA CTCTTCCATG TTCTGGATGC GGGTACCCTT GGTGGCACCA
    GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAACTA CACACACAGA GGAGTACAGT
    GGGGAGGAGA AAACGTGGAT GTGGTGGCAC AACTTCCGGA CTTTGTGTGA CTATAGTAAG
    AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGC
    TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACTA GCATTTTCCT GACCAATAAG
    AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTCA TCTTCCGGCT CCTCAAGTTG
    GAGGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCCTAC
    CTCCAATACC TGGAATACTT AAGCCAGAAC CGTCCTCCAC CTAATGCCTA TGAACTCTTT
    GCCAAGGGCT ATGAAGACTA TCTGCAGTCC CCGCTTCAGC CACTGATGGA CAATCTGGAA
    TCTCAGACAT ATGAAGTGTT TGAAAAGGAC CCCATCAAAT ACTCTCAGTA CCAGCAGGCC
    ATCTATAAAT GTCTGCTAGA CCGAGTACCA GAAGAGGAGA AGGATACCAA TGTCCAGGTA
    CTGATGGTGC TGGGAGCAGG ACGGGGACCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG
    CAGGCCGACC GGCGGATAAA GCTGTATGCT GTGGAGAAAA ACCCAAATGC CGTGGTGACG
    CTAGAGAACT GGCAGTTTGA AGAATGGGGA AGCCAAGTGA CCGTAGTCTC ATCAGACATG
    AGGGAATGGG TGGCTCCAGA GAAAGCAGAC ATCATTGTCA GTGAGCTTCT GGGCTCATTT
    GCTGACAATG AATTGTCGCC TGAGTGCCTG GATGGAGCCC AGCACTTCCT AAAAGATGAT
    GGTGTGAGCA TCCCCGGGGA GTACACTTCC TTTCTGGCTC CCATCTCTTC CTCCAAGCTG
    TACAATGAGG TCCGAGCCTG TAGGGAGAAG GACCGTGACC CTGAGGCCCA GTTTGAGATG
    CCTTATGTGG TACGGCTGCA CAACTTCCAC CAGCTCTCTG CACCCCAGCC CTGTTTCACC
    TTCAGCCATC CCAACAGAGA TCCTATGATT GACAACAACC GCTATTGCAC CTTGGAATTT
    CCTGTGGAGG TGAACACAGT ACTACATGGC TTTGCCGGCT ACTTTGAGAC TGTGCTTTAT
    CAGGACATCA CTCTGAGTAT CCGTCCAGAG ACTCACTCTC CTGGGATGTT CTCATGGTTT
    CCCATCCTCT TCCCTATTAA GCAGCCCATA ACGGTACGTG AAGGCCAAAC CATCTGTGTG
    CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA
    GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATATA CCATTGGCCT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23684
    Clone ID Related Accession (Same CDS sequence) NM_006109.5 , NM_006109.4
    Accession Version NM_006109.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AL833612.1 and AA496357.1. On Sep 24, 2013 this sequence version replaced NM_006109.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.129601.1, AF167572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG
    CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATTCGC
    AGGAACTCCG AGGCGGCCAT GTTACAGGAG CTGAATTTTG GTGCATATTT GGGTCTTCCA
    GCTTTCCTGC TGCCCCTTAA TCAGGAAGAT AACACCAACC TGGCCAGAGT TTTGACCAAC
    CACATCCACA CTGGCCATCA CTCTTCCATG TTCTGGATGC GGGTACCCTT GGTGGCACCA
    GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAACTA CACACACAGA GGAGTACAGT
    GGGGAGGAGA AAACGTGGAT GTGGTGGCAC AACTTCCGGA CTTTGTGTGA CTATAGTAAG
    AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGC
    TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACTA GCATTTTCCT GACCAATAAG
    AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTCA TCTTCCGGCT CCTCAAGTTG
    GAGGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCCTAC
    CTCCAATACC TGGAATACTT AAGCCAGAAC CGTCCTCCAC CTAATGCCTA TGAACTCTTT
    GCCAAGGGCT ATGAAGACTA TCTGCAGTCC CCGCTTCAGC CACTGATGGA CAATCTGGAA
    TCTCAGACAT ATGAAGTGTT TGAAAAGGAC CCCATCAAAT ACTCTCAGTA CCAGCAGGCC
    ATCTATAAAT GTCTGCTAGA CCGAGTACCA GAAGAGGAGA AGGATACCAA TGTCCAGGTA
    CTGATGGTGC TGGGAGCAGG ACGGGGACCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG
    CAGGCCGACC GGCGGATAAA GCTGTATGCT GTGGAGAAAA ACCCAAATGC CGTGGTGACG
    CTAGAGAACT GGCAGTTTGA AGAATGGGGA AGCCAAGTGA CCGTAGTCTC ATCAGACATG
    AGGGAATGGG TGGCTCCAGA GAAAGCAGAC ATCATTGTCA GTGAGCTTCT GGGCTCATTT
    GCTGACAATG AATTGTCGCC TGAGTGCCTG GATGGAGCCC AGCACTTCCT AAAAGATGAT
    GGTGTGAGCA TCCCCGGGGA GTACACTTCC TTTCTGGCTC CCATCTCTTC CTCCAAGCTG
    TACAATGAGG TCCGAGCCTG TAGGGAGAAG GACCGTGACC CTGAGGCCCA GTTTGAGATG
    CCTTATGTGG TACGGCTGCA CAACTTCCAC CAGCTCTCTG CACCCCAGCC CTGTTTCACC
    TTCAGCCATC CCAACAGAGA TCCTATGATT GACAACAACC GCTATTGCAC CTTGGAATTT
    CCTGTGGAGG TGAACACAGT ACTACATGGC TTTGCCGGCT ACTTTGAGAC TGTGCTTTAT
    CAGGACATCA CTCTGAGTAT CCGTCCAGAG ACTCACTCTC CTGGGATGTT CTCATGGTTT
    CCCATCCTCT TCCCTATTAA GCAGCCCATA ACGGTACGTG AAGGCCAAAC CATCTGTGTG
    CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA
    GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATATA CCATTGGCCT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006100.2
    CDS225..2138
    Translation

    Target ORF information:

    RefSeq Version NM_006109.4
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006109.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG
    CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATTCGC
    AGGAACTCCG AGGCGGCCAT GTTACAGGAG CTGAATTTTG GTGCATATTT GGGTCTTCCA
    GCTTTCCTGC TGCCCCTTAA TCAGGAAGAT AACACCAACC TGGCCAGAGT TTTGACCAAC
    CACATCCACA CTGGCCATCA CTCTTCCATG TTCTGGATGC GGGTACCCTT GGTGGCACCA
    GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAACTA CACACACAGA GGAGTACAGT
    GGGGAGGAGA AAACGTGGAT GTGGTGGCAC AACTTCCGGA CTTTGTGTGA CTATAGTAAG
    AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGC
    TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACTA GCATTTTCCT GACCAATAAG
    AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTCA TCTTCCGGCT CCTCAAGTTG
    GAGGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCCTAC
    CTCCAATACC TGGAATACTT AAGCCAGAAC CGTCCTCCAC CTAATGCCTA TGAACTCTTT
    GCCAAGGGCT ATGAAGACTA TCTGCAGTCC CCGCTTCAGC CACTGATGGA CAATCTGGAA
    TCTCAGACAT ATGAAGTGTT TGAAAAGGAC CCCATCAAAT ACTCTCAGTA CCAGCAGGCC
    ATCTATAAAT GTCTGCTAGA CCGAGTACCA GAAGAGGAGA AGGATACCAA TGTCCAGGTA
    CTGATGGTGC TGGGAGCAGG ACGGGGACCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG
    CAGGCCGACC GGCGGATAAA GCTGTATGCT GTGGAGAAAA ACCCAAATGC CGTGGTGACG
    CTAGAGAACT GGCAGTTTGA AGAATGGGGA AGCCAAGTGA CCGTAGTCTC ATCAGACATG
    AGGGAATGGG TGGCTCCAGA GAAAGCAGAC ATCATTGTCA GTGAGCTTCT GGGCTCATTT
    GCTGACAATG AATTGTCGCC TGAGTGCCTG GATGGAGCCC AGCACTTCCT AAAAGATGAT
    GGTGTGAGCA TCCCCGGGGA GTACACTTCC TTTCTGGCTC CCATCTCTTC CTCCAAGCTG
    TACAATGAGG TCCGAGCCTG TAGGGAGAAG GACCGTGACC CTGAGGCCCA GTTTGAGATG
    CCTTATGTGG TACGGCTGCA CAACTTCCAC CAGCTCTCTG CACCCCAGCC CTGTTTCACC
    TTCAGCCATC CCAACAGAGA TCCTATGATT GACAACAACC GCTATTGCAC CTTGGAATTT
    CCTGTGGAGG TGAACACAGT ACTACATGGC TTTGCCGGCT ACTTTGAGAC TGTGCTTTAT
    CAGGACATCA CTCTGAGTAT CCGTCCAGAG ACTCACTCTC CTGGGATGTT CTCATGGTTT
    CCCATCCTCT TCCCTATTAA GCAGCCCATA ACGGTACGTG AAGGCCAAAC CATCTGTGTG
    CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA
    GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATATA CCATTGGCCT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27546
    Clone ID Related Accession (Same CDS sequence) NM_001282955.2 , NM_001282955.1
    Accession Version NM_001282955.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AK302240.1 and AL833612.1. On May 31, 2019 this sequence version replaced NM_001282955.1. Transcript Variant: This variant (5) lacks an exon and uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1044477.1, SRR1803615.134544.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGC TTTCCTGCTG
    CCCCTTAATC AGGAAGATAA CACCAACCTG GCCAGAGTTT TGACCAACCA CATCCACACT
    GGCCATCACT CTTCCATGTT CTGGATGCGG GTACCCTTGG TGGCACCAGA GGACCTGAGA
    GATGATATAA TTGAGAATGC ACCAACTACA CACACAGAGG AGTACAGTGG GGAGGAGAAA
    ACGTGGATGT GGTGGCACAA CTTCCGGACT TTGTGTGACT ATAGTAAGAG GATTGCAGTG
    GCTCTTGAAA TTGGGGCTGA CCTCCCATCT AATCATGTCA TTGATCGCTG GCTTGGGGAG
    CCCATCAAAG CAGCCATTCT CCCCACTAGC ATTTTCCTGA CCAATAAGAA GGGATTTCCT
    GTTCTTTCTA AGATGCACCA GAGGCTCATC TTCCGGCTCC TCAAGTTGGA GGTGCAGTTC
    ATCATCACAG GCACCAACCA CCACTCAGAG AAGGAGTTCT GCTCCTACCT CCAATACCTG
    GAATACTTAA GCCAGAACCG TCCTCCACCT AATGCCTATG AACTCTTTGC CAAGGGCTAT
    GAAGACTATC TGCAGTCCCC GCTTCAGCCA CTGATGGACA ATCTGGAATC TCAGACATAT
    GAAGTGTTTG AAAAGGACCC CATCAAATAC TCTCAGTACC AGCAGGCCAT CTATAAATGT
    CTGCTAGACC GAGTACCAGA AGAGGAGAAG GATACCAATG TCCAGGTACT GATGGTGCTG
    GGAGCAGGAC GGGGACCCCT GGTGAACGCT TCCCTGCGGG CAGCCAAGCA GGCCGACCGG
    CGGATAAAGC TGTATGCTGT GGAGAAAAAC CCAAATGCCG TGGTGACGCT AGAGAACTGG
    CAGTTTGAAG AATGGGGAAG CCAAGTGACC GTAGTCTCAT CAGACATGAG GGAATGGGTG
    GCTCCAGAGA AAGCAGACAT CATTGTCAGT GAGCTTCTGG GCTCATTTGC TGACAATGAA
    TTGTCGCCTG AGTGCCTGGA TGGAGCCCAG CACTTCCTAA AAGATGATGG TGTGAGCATC
    CCCGGGGAGT ACACTTCCTT TCTGGCTCCC ATCTCTTCCT CCAAGCTGTA CAATGAGGTC
    CGAGCCTGTA GGGAGAAGGA CCGTGACCCT GAGGCCCAGT TTGAGATGCC TTATGTGGTA
    CGGCTGCACA ACTTCCACCA GCTCTCTGCA CCCCAGCCCT GTTTCACCTT CAGCCATCCC
    AACAGAGATC CTATGATTGA CAACAACCGC TATTGCACCT TGGAATTTCC TGTGGAGGTG
    AACACAGTAC TACATGGCTT TGCCGGCTAC TTTGAGACTG TGCTTTATCA GGACATCACT
    CTGAGTATCC GTCCAGAGAC TCACTCTCCT GGGATGTTCT CATGGTTTCC CATCCTCTTC
    CCTATTAAGC AGCCCATAAC GGTACGTGAA GGCCAAACCA TCTGTGTGCG TTTCTGGCGA
    TGCAGCAATT CCAAGAAGGT GTGGTATGAG TGGGCTGTGA CAGCACCAGT CTGTTCTGCT
    ATTCATAACC CCACAGGCCG CTCATATACC ATTGGCCTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269884.1
    CDS16..1797
    Translation

    Target ORF information:

    RefSeq Version NM_001282955.2
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282955.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGC TTTCCTGCTG
    CCCCTTAATC AGGAAGATAA CACCAACCTG GCCAGAGTTT TGACCAACCA CATCCACACT
    GGCCATCACT CTTCCATGTT CTGGATGCGG GTACCCTTGG TGGCACCAGA GGACCTGAGA
    GATGATATAA TTGAGAATGC ACCAACTACA CACACAGAGG AGTACAGTGG GGAGGAGAAA
    ACGTGGATGT GGTGGCACAA CTTCCGGACT TTGTGTGACT ATAGTAAGAG GATTGCAGTG
    GCTCTTGAAA TTGGGGCTGA CCTCCCATCT AATCATGTCA TTGATCGCTG GCTTGGGGAG
    CCCATCAAAG CAGCCATTCT CCCCACTAGC ATTTTCCTGA CCAATAAGAA GGGATTTCCT
    GTTCTTTCTA AGATGCACCA GAGGCTCATC TTCCGGCTCC TCAAGTTGGA GGTGCAGTTC
    ATCATCACAG GCACCAACCA CCACTCAGAG AAGGAGTTCT GCTCCTACCT CCAATACCTG
    GAATACTTAA GCCAGAACCG TCCTCCACCT AATGCCTATG AACTCTTTGC CAAGGGCTAT
    GAAGACTATC TGCAGTCCCC GCTTCAGCCA CTGATGGACA ATCTGGAATC TCAGACATAT
    GAAGTGTTTG AAAAGGACCC CATCAAATAC TCTCAGTACC AGCAGGCCAT CTATAAATGT
    CTGCTAGACC GAGTACCAGA AGAGGAGAAG GATACCAATG TCCAGGTACT GATGGTGCTG
    GGAGCAGGAC GGGGACCCCT GGTGAACGCT TCCCTGCGGG CAGCCAAGCA GGCCGACCGG
    CGGATAAAGC TGTATGCTGT GGAGAAAAAC CCAAATGCCG TGGTGACGCT AGAGAACTGG
    CAGTTTGAAG AATGGGGAAG CCAAGTGACC GTAGTCTCAT CAGACATGAG GGAATGGGTG
    GCTCCAGAGA AAGCAGACAT CATTGTCAGT GAGCTTCTGG GCTCATTTGC TGACAATGAA
    TTGTCGCCTG AGTGCCTGGA TGGAGCCCAG CACTTCCTAA AAGATGATGG TGTGAGCATC
    CCCGGGGAGT ACACTTCCTT TCTGGCTCCC ATCTCTTCCT CCAAGCTGTA CAATGAGGTC
    CGAGCCTGTA GGGAGAAGGA CCGTGACCCT GAGGCCCAGT TTGAGATGCC TTATGTGGTA
    CGGCTGCACA ACTTCCACCA GCTCTCTGCA CCCCAGCCCT GTTTCACCTT CAGCCATCCC
    AACAGAGATC CTATGATTGA CAACAACCGC TATTGCACCT TGGAATTTCC TGTGGAGGTG
    AACACAGTAC TACATGGCTT TGCCGGCTAC TTTGAGACTG TGCTTTATCA GGACATCACT
    CTGAGTATCC GTCCAGAGAC TCACTCTCCT GGGATGTTCT CATGGTTTCC CATCCTCTTC
    CCTATTAAGC AGCCCATAAC GGTACGTGAA GGCCAAACCA TCTGTGTGCG TTTCTGGCGA
    TGCAGCAATT CCAAGAAGGT GTGGTATGAG TGGGCTGTGA CAGCACCAGT CTGTTCTGCT
    ATTCATAACC CCACAGGCCG CTCATATACC ATTGGCCTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27546
    Clone ID Related Accession (Same CDS sequence) NM_001282955.2 , NM_001282955.1
    Accession Version NM_001282955.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-06
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AK302240.1, AL833612.1 and AA496357.1. On Sep 24, 2013 this sequence version replaced XM_005267259.1. Transcript Variant: This variant (5) lacks an exon and uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.419173.1, SRR1803615.134544.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGC TTTCCTGCTG
    CCCCTTAATC AGGAAGATAA CACCAACCTG GCCAGAGTTT TGACCAACCA CATCCACACT
    GGCCATCACT CTTCCATGTT CTGGATGCGG GTACCCTTGG TGGCACCAGA GGACCTGAGA
    GATGATATAA TTGAGAATGC ACCAACTACA CACACAGAGG AGTACAGTGG GGAGGAGAAA
    ACGTGGATGT GGTGGCACAA CTTCCGGACT TTGTGTGACT ATAGTAAGAG GATTGCAGTG
    GCTCTTGAAA TTGGGGCTGA CCTCCCATCT AATCATGTCA TTGATCGCTG GCTTGGGGAG
    CCCATCAAAG CAGCCATTCT CCCCACTAGC ATTTTCCTGA CCAATAAGAA GGGATTTCCT
    GTTCTTTCTA AGATGCACCA GAGGCTCATC TTCCGGCTCC TCAAGTTGGA GGTGCAGTTC
    ATCATCACAG GCACCAACCA CCACTCAGAG AAGGAGTTCT GCTCCTACCT CCAATACCTG
    GAATACTTAA GCCAGAACCG TCCTCCACCT AATGCCTATG AACTCTTTGC CAAGGGCTAT
    GAAGACTATC TGCAGTCCCC GCTTCAGCCA CTGATGGACA ATCTGGAATC TCAGACATAT
    GAAGTGTTTG AAAAGGACCC CATCAAATAC TCTCAGTACC AGCAGGCCAT CTATAAATGT
    CTGCTAGACC GAGTACCAGA AGAGGAGAAG GATACCAATG TCCAGGTACT GATGGTGCTG
    GGAGCAGGAC GGGGACCCCT GGTGAACGCT TCCCTGCGGG CAGCCAAGCA GGCCGACCGG
    CGGATAAAGC TGTATGCTGT GGAGAAAAAC CCAAATGCCG TGGTGACGCT AGAGAACTGG
    CAGTTTGAAG AATGGGGAAG CCAAGTGACC GTAGTCTCAT CAGACATGAG GGAATGGGTG
    GCTCCAGAGA AAGCAGACAT CATTGTCAGT GAGCTTCTGG GCTCATTTGC TGACAATGAA
    TTGTCGCCTG AGTGCCTGGA TGGAGCCCAG CACTTCCTAA AAGATGATGG TGTGAGCATC
    CCCGGGGAGT ACACTTCCTT TCTGGCTCCC ATCTCTTCCT CCAAGCTGTA CAATGAGGTC
    CGAGCCTGTA GGGAGAAGGA CCGTGACCCT GAGGCCCAGT TTGAGATGCC TTATGTGGTA
    CGGCTGCACA ACTTCCACCA GCTCTCTGCA CCCCAGCCCT GTTTCACCTT CAGCCATCCC
    AACAGAGATC CTATGATTGA CAACAACCGC TATTGCACCT TGGAATTTCC TGTGGAGGTG
    AACACAGTAC TACATGGCTT TGCCGGCTAC TTTGAGACTG TGCTTTATCA GGACATCACT
    CTGAGTATCC GTCCAGAGAC TCACTCTCCT GGGATGTTCT CATGGTTTCC CATCCTCTTC
    CCTATTAAGC AGCCCATAAC GGTACGTGAA GGCCAAACCA TCTGTGTGCG TTTCTGGCGA
    TGCAGCAATT CCAAGAAGGT GTGGTATGAG TGGGCTGTGA CAGCACCAGT CTGTTCTGCT
    ATTCATAACC CCACAGGCCG CTCATATACC ATTGGCCTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269884.1
    CDS225..2006
    Translation

    Target ORF information:

    RefSeq Version NM_001282955.1
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282955.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCGA TGGCGGTCGG GGGTGCTGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
    AATTGCGTCC CCGAAATAGC TGACACACTA GGGGCTGTGG CCAAGCAGGG GTTTGATTTC
    CTCTGCATGC CTGTCTTCCA TCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
    AATCGGCCCG GTCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGC TTTCCTGCTG
    CCCCTTAATC AGGAAGATAA CACCAACCTG GCCAGAGTTT TGACCAACCA CATCCACACT
    GGCCATCACT CTTCCATGTT CTGGATGCGG GTACCCTTGG TGGCACCAGA GGACCTGAGA
    GATGATATAA TTGAGAATGC ACCAACTACA CACACAGAGG AGTACAGTGG GGAGGAGAAA
    ACGTGGATGT GGTGGCACAA CTTCCGGACT TTGTGTGACT ATAGTAAGAG GATTGCAGTG
    GCTCTTGAAA TTGGGGCTGA CCTCCCATCT AATCATGTCA TTGATCGCTG GCTTGGGGAG
    CCCATCAAAG CAGCCATTCT CCCCACTAGC ATTTTCCTGA CCAATAAGAA GGGATTTCCT
    GTTCTTTCTA AGATGCACCA GAGGCTCATC TTCCGGCTCC TCAAGTTGGA GGTGCAGTTC
    ATCATCACAG GCACCAACCA CCACTCAGAG AAGGAGTTCT GCTCCTACCT CCAATACCTG
    GAATACTTAA GCCAGAACCG TCCTCCACCT AATGCCTATG AACTCTTTGC CAAGGGCTAT
    GAAGACTATC TGCAGTCCCC GCTTCAGCCA CTGATGGACA ATCTGGAATC TCAGACATAT
    GAAGTGTTTG AAAAGGACCC CATCAAATAC TCTCAGTACC AGCAGGCCAT CTATAAATGT
    CTGCTAGACC GAGTACCAGA AGAGGAGAAG GATACCAATG TCCAGGTACT GATGGTGCTG
    GGAGCAGGAC GGGGACCCCT GGTGAACGCT TCCCTGCGGG CAGCCAAGCA GGCCGACCGG
    CGGATAAAGC TGTATGCTGT GGAGAAAAAC CCAAATGCCG TGGTGACGCT AGAGAACTGG
    CAGTTTGAAG AATGGGGAAG CCAAGTGACC GTAGTCTCAT CAGACATGAG GGAATGGGTG
    GCTCCAGAGA AAGCAGACAT CATTGTCAGT GAGCTTCTGG GCTCATTTGC TGACAATGAA
    TTGTCGCCTG AGTGCCTGGA TGGAGCCCAG CACTTCCTAA AAGATGATGG TGTGAGCATC
    CCCGGGGAGT ACACTTCCTT TCTGGCTCCC ATCTCTTCCT CCAAGCTGTA CAATGAGGTC
    CGAGCCTGTA GGGAGAAGGA CCGTGACCCT GAGGCCCAGT TTGAGATGCC TTATGTGGTA
    CGGCTGCACA ACTTCCACCA GCTCTCTGCA CCCCAGCCCT GTTTCACCTT CAGCCATCCC
    AACAGAGATC CTATGATTGA CAACAACCGC TATTGCACCT TGGAATTTCC TGTGGAGGTG
    AACACAGTAC TACATGGCTT TGCCGGCTAC TTTGAGACTG TGCTTTATCA GGACATCACT
    CTGAGTATCC GTCCAGAGAC TCACTCTCCT GGGATGTTCT CATGGTTTCC CATCCTCTTC
    CCTATTAAGC AGCCCATAAC GGTACGTGAA GGCCAAACCA TCTGTGTGCG TTTCTGGCGA
    TGCAGCAATT CCAAGAAGGT GTGGTATGAG TGGGCTGTGA CAGCACCAGT CTGTTCTGCT
    ATTCATAACC CCACAGGCCG CTCATATACC ATTGGCCTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27484
    Clone ID Related Accession (Same CDS sequence) NM_001282954.1 , NM_001282954.2 , XM_017020927.1
    Accession Version NM_001282954.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-06
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AK300863.1, AL833612.1 and AA496357.1. Transcript Variant: This variant (4) contains multiple differences in the 5' coding region, compared to variant 1, including initiation of translation at a downstream in-frame start codon. The encoded isoform (d) has a shorter N-terminus than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.154544.1, SRR1803614.66898.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTACAGG AGCTGAATTT TGGTGCATAT TTGGGTCTTC CAGCTTTCCT GCTGCCCCTT 
    AATCAGGAAG ATAACACCAA CCTGGCCAGA GTTTTGACCA ACCACATCCA CACTGGCCAT
    CACTCTTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCAC CAGAGGACCT GAGAGATGAT
    ATAATTGAGA ATGCACCAAC TACACACACA GAGGAGTACA GTGGGGAGGA GAAAACGTGG
    ATGTGGTGGC ACAACTTCCG GACTTTGTGT GACTATAGTA AGAGGATTGC AGTGGCTCTT
    GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GCTGGCTTGG GGAGCCCATC
    AAAGCAGCCA TTCTCCCCAC TAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT
    TCTAAGATGC ACCAGAGGCT CATCTTCCGG CTCCTCAAGT TGGAGGTGCA GTTCATCATC
    ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCCT ACCTCCAATA CCTGGAATAC
    TTAAGCCAGA ACCGTCCTCC ACCTAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC
    TATCTGCAGT CCCCGCTTCA GCCACTGATG GACAATCTGG AATCTCAGAC ATATGAAGTG
    TTTGAAAAGG ACCCCATCAA ATACTCTCAG TACCAGCAGG CCATCTATAA ATGTCTGCTA
    GACCGAGTAC CAGAAGAGGA GAAGGATACC AATGTCCAGG TACTGATGGT GCTGGGAGCA
    GGACGGGGAC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCCGA CCGGCGGATA
    AAGCTGTATG CTGTGGAGAA AAACCCAAAT GCCGTGGTGA CGCTAGAGAA CTGGCAGTTT
    GAAGAATGGG GAAGCCAAGT GACCGTAGTC TCATCAGACA TGAGGGAATG GGTGGCTCCA
    GAGAAAGCAG ACATCATTGT CAGTGAGCTT CTGGGCTCAT TTGCTGACAA TGAATTGTCG
    CCTGAGTGCC TGGATGGAGC CCAGCACTTC CTAAAAGATG ATGGTGTGAG CATCCCCGGG
    GAGTACACTT CCTTTCTGGC TCCCATCTCT TCCTCCAAGC TGTACAATGA GGTCCGAGCC
    TGTAGGGAGA AGGACCGTGA CCCTGAGGCC CAGTTTGAGA TGCCTTATGT GGTACGGCTG
    CACAACTTCC ACCAGCTCTC TGCACCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAACAGA
    GATCCTATGA TTGACAACAA CCGCTATTGC ACCTTGGAAT TTCCTGTGGA GGTGAACACA
    GTACTACATG GCTTTGCCGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACTCTGAGT
    ATCCGTCCAG AGACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCTATT
    AAGCAGCCCA TAACGGTACG TGAAGGCCAA ACCATCTGTG TGCGTTTCTG GCGATGCAGC
    AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT
    AACCCCACAG GCCGCTCATA TACCATTGGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269883.1
    CDS509..2104
    Translation

    Target ORF information:

    RefSeq Version NM_001282954.1
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282954.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTACAGG AGCTGAATTT TGGTGCATAT TTGGGTCTTC CAGCTTTCCT GCTGCCCCTT 
    AATCAGGAAG ATAACACCAA CCTGGCCAGA GTTTTGACCA ACCACATCCA CACTGGCCAT
    CACTCTTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCAC CAGAGGACCT GAGAGATGAT
    ATAATTGAGA ATGCACCAAC TACACACACA GAGGAGTACA GTGGGGAGGA GAAAACGTGG
    ATGTGGTGGC ACAACTTCCG GACTTTGTGT GACTATAGTA AGAGGATTGC AGTGGCTCTT
    GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GCTGGCTTGG GGAGCCCATC
    AAAGCAGCCA TTCTCCCCAC TAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT
    TCTAAGATGC ACCAGAGGCT CATCTTCCGG CTCCTCAAGT TGGAGGTGCA GTTCATCATC
    ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCCT ACCTCCAATA CCTGGAATAC
    TTAAGCCAGA ACCGTCCTCC ACCTAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC
    TATCTGCAGT CCCCGCTTCA GCCACTGATG GACAATCTGG AATCTCAGAC ATATGAAGTG
    TTTGAAAAGG ACCCCATCAA ATACTCTCAG TACCAGCAGG CCATCTATAA ATGTCTGCTA
    GACCGAGTAC CAGAAGAGGA GAAGGATACC AATGTCCAGG TACTGATGGT GCTGGGAGCA
    GGACGGGGAC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCCGA CCGGCGGATA
    AAGCTGTATG CTGTGGAGAA AAACCCAAAT GCCGTGGTGA CGCTAGAGAA CTGGCAGTTT
    GAAGAATGGG GAAGCCAAGT GACCGTAGTC TCATCAGACA TGAGGGAATG GGTGGCTCCA
    GAGAAAGCAG ACATCATTGT CAGTGAGCTT CTGGGCTCAT TTGCTGACAA TGAATTGTCG
    CCTGAGTGCC TGGATGGAGC CCAGCACTTC CTAAAAGATG ATGGTGTGAG CATCCCCGGG
    GAGTACACTT CCTTTCTGGC TCCCATCTCT TCCTCCAAGC TGTACAATGA GGTCCGAGCC
    TGTAGGGAGA AGGACCGTGA CCCTGAGGCC CAGTTTGAGA TGCCTTATGT GGTACGGCTG
    CACAACTTCC ACCAGCTCTC TGCACCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAACAGA
    GATCCTATGA TTGACAACAA CCGCTATTGC ACCTTGGAAT TTCCTGTGGA GGTGAACACA
    GTACTACATG GCTTTGCCGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACTCTGAGT
    ATCCGTCCAG AGACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCTATT
    AAGCAGCCCA TAACGGTACG TGAAGGCCAA ACCATCTGTG TGCGTTTCTG GCGATGCAGC
    AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT
    AACCCCACAG GCCGCTCATA TACCATTGGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27484
    Clone ID Related Accession (Same CDS sequence) NM_001282954.1 , NM_001282954.2 , XM_017020927.1
    Accession Version NM_001282954.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AK300863.1 and AL833612.1. On May 31, 2019 this sequence version replaced NM_001282954.1. Transcript Variant: This variant (4) contains multiple differences in the 5' coding region, compared to variant 1, including initiation of translation at a downstream in-frame start codon. The encoded isoform (d) has a shorter N-terminus than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.154544.1, SRR1803614.171764.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTACAGG AGCTGAATTT TGGTGCATAT TTGGGTCTTC CAGCTTTCCT GCTGCCCCTT 
    AATCAGGAAG ATAACACCAA CCTGGCCAGA GTTTTGACCA ACCACATCCA CACTGGCCAT
    CACTCTTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCAC CAGAGGACCT GAGAGATGAT
    ATAATTGAGA ATGCACCAAC TACACACACA GAGGAGTACA GTGGGGAGGA GAAAACGTGG
    ATGTGGTGGC ACAACTTCCG GACTTTGTGT GACTATAGTA AGAGGATTGC AGTGGCTCTT
    GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GCTGGCTTGG GGAGCCCATC
    AAAGCAGCCA TTCTCCCCAC TAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT
    TCTAAGATGC ACCAGAGGCT CATCTTCCGG CTCCTCAAGT TGGAGGTGCA GTTCATCATC
    ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCCT ACCTCCAATA CCTGGAATAC
    TTAAGCCAGA ACCGTCCTCC ACCTAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC
    TATCTGCAGT CCCCGCTTCA GCCACTGATG GACAATCTGG AATCTCAGAC ATATGAAGTG
    TTTGAAAAGG ACCCCATCAA ATACTCTCAG TACCAGCAGG CCATCTATAA ATGTCTGCTA
    GACCGAGTAC CAGAAGAGGA GAAGGATACC AATGTCCAGG TACTGATGGT GCTGGGAGCA
    GGACGGGGAC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCCGA CCGGCGGATA
    AAGCTGTATG CTGTGGAGAA AAACCCAAAT GCCGTGGTGA CGCTAGAGAA CTGGCAGTTT
    GAAGAATGGG GAAGCCAAGT GACCGTAGTC TCATCAGACA TGAGGGAATG GGTGGCTCCA
    GAGAAAGCAG ACATCATTGT CAGTGAGCTT CTGGGCTCAT TTGCTGACAA TGAATTGTCG
    CCTGAGTGCC TGGATGGAGC CCAGCACTTC CTAAAAGATG ATGGTGTGAG CATCCCCGGG
    GAGTACACTT CCTTTCTGGC TCCCATCTCT TCCTCCAAGC TGTACAATGA GGTCCGAGCC
    TGTAGGGAGA AGGACCGTGA CCCTGAGGCC CAGTTTGAGA TGCCTTATGT GGTACGGCTG
    CACAACTTCC ACCAGCTCTC TGCACCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAACAGA
    GATCCTATGA TTGACAACAA CCGCTATTGC ACCTTGGAAT TTCCTGTGGA GGTGAACACA
    GTACTACATG GCTTTGCCGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACTCTGAGT
    ATCCGTCCAG AGACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCTATT
    AAGCAGCCCA TAACGGTACG TGAAGGCCAA ACCATCTGTG TGCGTTTCTG GCGATGCAGC
    AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT
    AACCCCACAG GCCGCTCATA TACCATTGGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269883.1
    CDS300..1895
    Translation

    Target ORF information:

    RefSeq Version NM_001282954.2
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282954.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTACAGG AGCTGAATTT TGGTGCATAT TTGGGTCTTC CAGCTTTCCT GCTGCCCCTT 
    AATCAGGAAG ATAACACCAA CCTGGCCAGA GTTTTGACCA ACCACATCCA CACTGGCCAT
    CACTCTTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCAC CAGAGGACCT GAGAGATGAT
    ATAATTGAGA ATGCACCAAC TACACACACA GAGGAGTACA GTGGGGAGGA GAAAACGTGG
    ATGTGGTGGC ACAACTTCCG GACTTTGTGT GACTATAGTA AGAGGATTGC AGTGGCTCTT
    GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GCTGGCTTGG GGAGCCCATC
    AAAGCAGCCA TTCTCCCCAC TAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT
    TCTAAGATGC ACCAGAGGCT CATCTTCCGG CTCCTCAAGT TGGAGGTGCA GTTCATCATC
    ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCCT ACCTCCAATA CCTGGAATAC
    TTAAGCCAGA ACCGTCCTCC ACCTAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC
    TATCTGCAGT CCCCGCTTCA GCCACTGATG GACAATCTGG AATCTCAGAC ATATGAAGTG
    TTTGAAAAGG ACCCCATCAA ATACTCTCAG TACCAGCAGG CCATCTATAA ATGTCTGCTA
    GACCGAGTAC CAGAAGAGGA GAAGGATACC AATGTCCAGG TACTGATGGT GCTGGGAGCA
    GGACGGGGAC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCCGA CCGGCGGATA
    AAGCTGTATG CTGTGGAGAA AAACCCAAAT GCCGTGGTGA CGCTAGAGAA CTGGCAGTTT
    GAAGAATGGG GAAGCCAAGT GACCGTAGTC TCATCAGACA TGAGGGAATG GGTGGCTCCA
    GAGAAAGCAG ACATCATTGT CAGTGAGCTT CTGGGCTCAT TTGCTGACAA TGAATTGTCG
    CCTGAGTGCC TGGATGGAGC CCAGCACTTC CTAAAAGATG ATGGTGTGAG CATCCCCGGG
    GAGTACACTT CCTTTCTGGC TCCCATCTCT TCCTCCAAGC TGTACAATGA GGTCCGAGCC
    TGTAGGGAGA AGGACCGTGA CCCTGAGGCC CAGTTTGAGA TGCCTTATGT GGTACGGCTG
    CACAACTTCC ACCAGCTCTC TGCACCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAACAGA
    GATCCTATGA TTGACAACAA CCGCTATTGC ACCTTGGAAT TTCCTGTGGA GGTGAACACA
    GTACTACATG GCTTTGCCGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACTCTGAGT
    ATCCGTCCAG AGACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCTATT
    AAGCAGCCCA TAACGGTACG TGAAGGCCAA ACCATCTGTG TGCGTTTCTG GCGATGCAGC
    AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT
    AACCCCACAG GCCGCTCATA TACCATTGGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27659
    Clone ID Related Accession (Same CDS sequence) NM_001039619.3 , NM_001039619.2
    Accession Version NM_001039619.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK075251.1 and AK027737.1. On May 31, 2019 this sequence version replaced NM_001039619.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.533862.1, SRR1660807.6489.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGGCCATG TTACAGGAGC TGAATTTTGG TGCATATTTG
    GGTCTTCCAG CTTTCCTGCT GCCCCTTAAT CAGGAAGATA ACACCAACCT GGCCAGAGTT
    TTGACCAACC ACATCCACAC TGGCCATCAC TCTTCCATGT TCTGGATGCG GGTACCCTTG
    GTGGCACCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAACTAC ACACACAGAG
    GAGTACAGTG GGGAGGAGAA AACGTGGATG TGGTGGCACA ACTTCCGGAC TTTGTGTGAC
    TATAGTAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC
    ATTGATCGCT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACTAG CATTTTCCTG
    ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTCAT CTTCCGGCTC
    CTCAAGTTGG AGGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC
    TGCTCCTACC TCCAATACCT GGAATACTTA AGCCAGAACC GTCCTCCACC TAATGCCTAT
    GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCCC CGCTTCAGCC ACTGATGGAC
    AATCTGGAAT CTCAGACATA TGAAGTGTTT GAAAAGGACC CCATCAAATA CTCTCAGTAC
    CAGCAGGCCA TCTATAAATG TCTGCTAGAC CGAGTACCAG AAGAGGAGAA GGATACCAAT
    GTCCAGGTAC TGATGGTGCT GGGAGCAGGA CGGGGACCCC TGGTGAACGC TTCCCTGCGG
    GCAGCCAAGC AGGCCGACCG GCGGATAAAG CTGTATGCTG TGGAGAAAAA CCCAAATGCC
    GTGGTGACGC TAGAGAACTG GCAGTTTGAA GAATGGGGAA GCCAAGTGAC CGTAGTCTCA
    TCAGACATGA GGGAATGGGT GGCTCCAGAG AAAGCAGACA TCATTGTCAG TGAGCTTCTG
    GGCTCATTTG CTGACAATGA ATTGTCGCCT GAGTGCCTGG ATGGAGCCCA GCACTTCCTA
    AAAGATGATG GTGTGAGCAT CCCCGGGGAG TACACTTCCT TTCTGGCTCC CATCTCTTCC
    TCCAAGCTGT ACAATGAGGT CCGAGCCTGT AGGGAGAAGG ACCGTGACCC TGAGGCCCAG
    TTTGAGATGC CTTATGTGGT ACGGCTGCAC AACTTCCACC AGCTCTCTGC ACCCCAGCCC
    TGTTTCACCT TCAGCCATCC CAACAGAGAT CCTATGATTG ACAACAACCG CTATTGCACC
    TTGGAATTTC CTGTGGAGGT GAACACAGTA CTACATGGCT TTGCCGGCTA CTTTGAGACT
    GTGCTTTATC AGGACATCAC TCTGAGTATC CGTCCAGAGA CTCACTCTCC TGGGATGTTC
    TCATGGTTTC CCATCCTCTT CCCTATTAAG CAGCCCATAA CGGTACGTGA AGGCCAAACC
    ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG
    ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATATAC CATTGGCCTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034708.1
    CDS152..2014
    Translation

    Target ORF information:

    RefSeq Version NM_001039619.3
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001039619.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGGCCATG TTACAGGAGC TGAATTTTGG TGCATATTTG
    GGTCTTCCAG CTTTCCTGCT GCCCCTTAAT CAGGAAGATA ACACCAACCT GGCCAGAGTT
    TTGACCAACC ACATCCACAC TGGCCATCAC TCTTCCATGT TCTGGATGCG GGTACCCTTG
    GTGGCACCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAACTAC ACACACAGAG
    GAGTACAGTG GGGAGGAGAA AACGTGGATG TGGTGGCACA ACTTCCGGAC TTTGTGTGAC
    TATAGTAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC
    ATTGATCGCT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACTAG CATTTTCCTG
    ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTCAT CTTCCGGCTC
    CTCAAGTTGG AGGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC
    TGCTCCTACC TCCAATACCT GGAATACTTA AGCCAGAACC GTCCTCCACC TAATGCCTAT
    GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCCC CGCTTCAGCC ACTGATGGAC
    AATCTGGAAT CTCAGACATA TGAAGTGTTT GAAAAGGACC CCATCAAATA CTCTCAGTAC
    CAGCAGGCCA TCTATAAATG TCTGCTAGAC CGAGTACCAG AAGAGGAGAA GGATACCAAT
    GTCCAGGTAC TGATGGTGCT GGGAGCAGGA CGGGGACCCC TGGTGAACGC TTCCCTGCGG
    GCAGCCAAGC AGGCCGACCG GCGGATAAAG CTGTATGCTG TGGAGAAAAA CCCAAATGCC
    GTGGTGACGC TAGAGAACTG GCAGTTTGAA GAATGGGGAA GCCAAGTGAC CGTAGTCTCA
    TCAGACATGA GGGAATGGGT GGCTCCAGAG AAAGCAGACA TCATTGTCAG TGAGCTTCTG
    GGCTCATTTG CTGACAATGA ATTGTCGCCT GAGTGCCTGG ATGGAGCCCA GCACTTCCTA
    AAAGATGATG GTGTGAGCAT CCCCGGGGAG TACACTTCCT TTCTGGCTCC CATCTCTTCC
    TCCAAGCTGT ACAATGAGGT CCGAGCCTGT AGGGAGAAGG ACCGTGACCC TGAGGCCCAG
    TTTGAGATGC CTTATGTGGT ACGGCTGCAC AACTTCCACC AGCTCTCTGC ACCCCAGCCC
    TGTTTCACCT TCAGCCATCC CAACAGAGAT CCTATGATTG ACAACAACCG CTATTGCACC
    TTGGAATTTC CTGTGGAGGT GAACACAGTA CTACATGGCT TTGCCGGCTA CTTTGAGACT
    GTGCTTTATC AGGACATCAC TCTGAGTATC CGTCCAGAGA CTCACTCTCC TGGGATGTTC
    TCATGGTTTC CCATCCTCTT CCCTATTAAG CAGCCCATAA CGGTACGTGA AGGCCAAACC
    ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG
    ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATATAC CATTGGCCTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27659
    Clone ID Related Accession (Same CDS sequence) NM_001039619.3 , NM_001039619.2
    Accession Version NM_001039619.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-06
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AK075251.1, AK027737.1 and AA496357.1. On Sep 24, 2013 this sequence version replaced NM_001039619.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.65684.1, AK075251.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGGCCATG TTACAGGAGC TGAATTTTGG TGCATATTTG
    GGTCTTCCAG CTTTCCTGCT GCCCCTTAAT CAGGAAGATA ACACCAACCT GGCCAGAGTT
    TTGACCAACC ACATCCACAC TGGCCATCAC TCTTCCATGT TCTGGATGCG GGTACCCTTG
    GTGGCACCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAACTAC ACACACAGAG
    GAGTACAGTG GGGAGGAGAA AACGTGGATG TGGTGGCACA ACTTCCGGAC TTTGTGTGAC
    TATAGTAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC
    ATTGATCGCT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACTAG CATTTTCCTG
    ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTCAT CTTCCGGCTC
    CTCAAGTTGG AGGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC
    TGCTCCTACC TCCAATACCT GGAATACTTA AGCCAGAACC GTCCTCCACC TAATGCCTAT
    GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCCC CGCTTCAGCC ACTGATGGAC
    AATCTGGAAT CTCAGACATA TGAAGTGTTT GAAAAGGACC CCATCAAATA CTCTCAGTAC
    CAGCAGGCCA TCTATAAATG TCTGCTAGAC CGAGTACCAG AAGAGGAGAA GGATACCAAT
    GTCCAGGTAC TGATGGTGCT GGGAGCAGGA CGGGGACCCC TGGTGAACGC TTCCCTGCGG
    GCAGCCAAGC AGGCCGACCG GCGGATAAAG CTGTATGCTG TGGAGAAAAA CCCAAATGCC
    GTGGTGACGC TAGAGAACTG GCAGTTTGAA GAATGGGGAA GCCAAGTGAC CGTAGTCTCA
    TCAGACATGA GGGAATGGGT GGCTCCAGAG AAAGCAGACA TCATTGTCAG TGAGCTTCTG
    GGCTCATTTG CTGACAATGA ATTGTCGCCT GAGTGCCTGG ATGGAGCCCA GCACTTCCTA
    AAAGATGATG GTGTGAGCAT CCCCGGGGAG TACACTTCCT TTCTGGCTCC CATCTCTTCC
    TCCAAGCTGT ACAATGAGGT CCGAGCCTGT AGGGAGAAGG ACCGTGACCC TGAGGCCCAG
    TTTGAGATGC CTTATGTGGT ACGGCTGCAC AACTTCCACC AGCTCTCTGC ACCCCAGCCC
    TGTTTCACCT TCAGCCATCC CAACAGAGAT CCTATGATTG ACAACAACCG CTATTGCACC
    TTGGAATTTC CTGTGGAGGT GAACACAGTA CTACATGGCT TTGCCGGCTA CTTTGAGACT
    GTGCTTTATC AGGACATCAC TCTGAGTATC CGTCCAGAGA CTCACTCTCC TGGGATGTTC
    TCATGGTTTC CCATCCTCTT CCCTATTAAG CAGCCCATAA CGGTACGTGA AGGCCAAACC
    ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG
    ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATATAC CATTGGCCTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034708.1
    CDS361..2223
    Translation

    Target ORF information:

    RefSeq Version NM_001039619.2
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001039619.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGGCCATG TTACAGGAGC TGAATTTTGG TGCATATTTG
    GGTCTTCCAG CTTTCCTGCT GCCCCTTAAT CAGGAAGATA ACACCAACCT GGCCAGAGTT
    TTGACCAACC ACATCCACAC TGGCCATCAC TCTTCCATGT TCTGGATGCG GGTACCCTTG
    GTGGCACCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAACTAC ACACACAGAG
    GAGTACAGTG GGGAGGAGAA AACGTGGATG TGGTGGCACA ACTTCCGGAC TTTGTGTGAC
    TATAGTAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC
    ATTGATCGCT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACTAG CATTTTCCTG
    ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTCAT CTTCCGGCTC
    CTCAAGTTGG AGGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC
    TGCTCCTACC TCCAATACCT GGAATACTTA AGCCAGAACC GTCCTCCACC TAATGCCTAT
    GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCCC CGCTTCAGCC ACTGATGGAC
    AATCTGGAAT CTCAGACATA TGAAGTGTTT GAAAAGGACC CCATCAAATA CTCTCAGTAC
    CAGCAGGCCA TCTATAAATG TCTGCTAGAC CGAGTACCAG AAGAGGAGAA GGATACCAAT
    GTCCAGGTAC TGATGGTGCT GGGAGCAGGA CGGGGACCCC TGGTGAACGC TTCCCTGCGG
    GCAGCCAAGC AGGCCGACCG GCGGATAAAG CTGTATGCTG TGGAGAAAAA CCCAAATGCC
    GTGGTGACGC TAGAGAACTG GCAGTTTGAA GAATGGGGAA GCCAAGTGAC CGTAGTCTCA
    TCAGACATGA GGGAATGGGT GGCTCCAGAG AAAGCAGACA TCATTGTCAG TGAGCTTCTG
    GGCTCATTTG CTGACAATGA ATTGTCGCCT GAGTGCCTGG ATGGAGCCCA GCACTTCCTA
    AAAGATGATG GTGTGAGCAT CCCCGGGGAG TACACTTCCT TTCTGGCTCC CATCTCTTCC
    TCCAAGCTGT ACAATGAGGT CCGAGCCTGT AGGGAGAAGG ACCGTGACCC TGAGGCCCAG
    TTTGAGATGC CTTATGTGGT ACGGCTGCAC AACTTCCACC AGCTCTCTGC ACCCCAGCCC
    TGTTTCACCT TCAGCCATCC CAACAGAGAT CCTATGATTG ACAACAACCG CTATTGCACC
    TTGGAATTTC CTGTGGAGGT GAACACAGTA CTACATGGCT TTGCCGGCTA CTTTGAGACT
    GTGCTTTATC AGGACATCAC TCTGAGTATC CGTCCAGAGA CTCACTCTCC TGGGATGTTC
    TCATGGTTTC CCATCCTCTT CCCTATTAAG CAGCCCATAA CGGTACGTGA AGGCCAAACC
    ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG
    ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATATAC CATTGGCCTC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27484
    Clone ID Related Accession (Same CDS sequence) NM_001282954.1 , NM_001282954.2 , XM_017020927.1
    Accession Version XM_017020927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTACAGG AGCTGAATTT TGGTGCATAT TTGGGTCTTC CAGCTTTCCT GCTGCCCCTT 
    AATCAGGAAG ATAACACCAA CCTGGCCAGA GTTTTGACCA ACCACATCCA CACTGGCCAT
    CACTCTTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCAC CAGAGGACCT GAGAGATGAT
    ATAATTGAGA ATGCACCAAC TACACACACA GAGGAGTACA GTGGGGAGGA GAAAACGTGG
    ATGTGGTGGC ACAACTTCCG GACTTTGTGT GACTATAGTA AGAGGATTGC AGTGGCTCTT
    GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GCTGGCTTGG GGAGCCCATC
    AAAGCAGCCA TTCTCCCCAC TAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT
    TCTAAGATGC ACCAGAGGCT CATCTTCCGG CTCCTCAAGT TGGAGGTGCA GTTCATCATC
    ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCCT ACCTCCAATA CCTGGAATAC
    TTAAGCCAGA ACCGTCCTCC ACCTAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC
    TATCTGCAGT CCCCGCTTCA GCCACTGATG GACAATCTGG AATCTCAGAC ATATGAAGTG
    TTTGAAAAGG ACCCCATCAA ATACTCTCAG TACCAGCAGG CCATCTATAA ATGTCTGCTA
    GACCGAGTAC CAGAAGAGGA GAAGGATACC AATGTCCAGG TACTGATGGT GCTGGGAGCA
    GGACGGGGAC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCCGA CCGGCGGATA
    AAGCTGTATG CTGTGGAGAA AAACCCAAAT GCCGTGGTGA CGCTAGAGAA CTGGCAGTTT
    GAAGAATGGG GAAGCCAAGT GACCGTAGTC TCATCAGACA TGAGGGAATG GGTGGCTCCA
    GAGAAAGCAG ACATCATTGT CAGTGAGCTT CTGGGCTCAT TTGCTGACAA TGAATTGTCG
    CCTGAGTGCC TGGATGGAGC CCAGCACTTC CTAAAAGATG ATGGTGTGAG CATCCCCGGG
    GAGTACACTT CCTTTCTGGC TCCCATCTCT TCCTCCAAGC TGTACAATGA GGTCCGAGCC
    TGTAGGGAGA AGGACCGTGA CCCTGAGGCC CAGTTTGAGA TGCCTTATGT GGTACGGCTG
    CACAACTTCC ACCAGCTCTC TGCACCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAACAGA
    GATCCTATGA TTGACAACAA CCGCTATTGC ACCTTGGAAT TTCCTGTGGA GGTGAACACA
    GTACTACATG GCTTTGCCGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACTCTGAGT
    ATCCGTCCAG AGACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCTATT
    AAGCAGCCCA TAACGGTACG TGAAGGCCAA ACCATCTGTG TGCGTTTCTG GCGATGCAGC
    AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT
    AACCCCACAG GCCGCTCATA TACCATTGGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876416.1
    CDS267..1862
    Translation

    Target ORF information:

    RefSeq Version XM_017020927.1
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTACAGG AGCTGAATTT TGGTGCATAT TTGGGTCTTC CAGCTTTCCT GCTGCCCCTT 
    AATCAGGAAG ATAACACCAA CCTGGCCAGA GTTTTGACCA ACCACATCCA CACTGGCCAT
    CACTCTTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCAC CAGAGGACCT GAGAGATGAT
    ATAATTGAGA ATGCACCAAC TACACACACA GAGGAGTACA GTGGGGAGGA GAAAACGTGG
    ATGTGGTGGC ACAACTTCCG GACTTTGTGT GACTATAGTA AGAGGATTGC AGTGGCTCTT
    GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GCTGGCTTGG GGAGCCCATC
    AAAGCAGCCA TTCTCCCCAC TAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT
    TCTAAGATGC ACCAGAGGCT CATCTTCCGG CTCCTCAAGT TGGAGGTGCA GTTCATCATC
    ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCCT ACCTCCAATA CCTGGAATAC
    TTAAGCCAGA ACCGTCCTCC ACCTAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC
    TATCTGCAGT CCCCGCTTCA GCCACTGATG GACAATCTGG AATCTCAGAC ATATGAAGTG
    TTTGAAAAGG ACCCCATCAA ATACTCTCAG TACCAGCAGG CCATCTATAA ATGTCTGCTA
    GACCGAGTAC CAGAAGAGGA GAAGGATACC AATGTCCAGG TACTGATGGT GCTGGGAGCA
    GGACGGGGAC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCCGA CCGGCGGATA
    AAGCTGTATG CTGTGGAGAA AAACCCAAAT GCCGTGGTGA CGCTAGAGAA CTGGCAGTTT
    GAAGAATGGG GAAGCCAAGT GACCGTAGTC TCATCAGACA TGAGGGAATG GGTGGCTCCA
    GAGAAAGCAG ACATCATTGT CAGTGAGCTT CTGGGCTCAT TTGCTGACAA TGAATTGTCG
    CCTGAGTGCC TGGATGGAGC CCAGCACTTC CTAAAAGATG ATGGTGTGAG CATCCCCGGG
    GAGTACACTT CCTTTCTGGC TCCCATCTCT TCCTCCAAGC TGTACAATGA GGTCCGAGCC
    TGTAGGGAGA AGGACCGTGA CCCTGAGGCC CAGTTTGAGA TGCCTTATGT GGTACGGCTG
    CACAACTTCC ACCAGCTCTC TGCACCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAACAGA
    GATCCTATGA TTGACAACAA CCGCTATTGC ACCTTGGAAT TTCCTGTGGA GGTGAACACA
    GTACTACATG GCTTTGCCGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACTCTGAGT
    ATCCGTCCAG AGACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCTATT
    AAGCAGCCCA TAACGGTACG TGAAGGCCAA ACCATCTGTG TGCGTTTCTG GCGATGCAGC
    AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT
    AACCCCACAG GCCGCTCATA TACCATTGGC CTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23914
    Clone ID Related Accession (Same CDS sequence) NM_001282956.1 , NM_001282956.2
    Accession Version NM_001282956.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-06
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, BC005820.2, AL833612.1 and AA496357.1. On Sep 24, 2013 this sequence version replaced XM_005267261.1. Transcript Variant: This variant (6) contains multiple differences in the 5' coding region, compared to variant 1, including initiation of translation at an alternate start codon. The encoded isoform (f) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005820.2, SRR1803616.178198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGTTGGAG GTGCAGTTCA TCATCACAGG CACCAACCAC
    CACTCAGAGA AGGAGTTCTG CTCCTACCTC CAATACCTGG AATACTTAAG CCAGAACCGT
    CCTCCACCTA ATGCCTATGA ACTCTTTGCC AAGGGCTATG AAGACTATCT GCAGTCCCCG
    CTTCAGCCAC TGATGGACAA TCTGGAATCT CAGACATATG AAGTGTTTGA AAAGGACCCC
    ATCAAATACT CTCAGTACCA GCAGGCCATC TATAAATGTC TGCTAGACCG AGTACCAGAA
    GAGGAGAAGG ATACCAATGT CCAGGTACTG ATGGTGCTGG GAGCAGGACG GGGACCCCTG
    GTGAACGCTT CCCTGCGGGC AGCCAAGCAG GCCGACCGGC GGATAAAGCT GTATGCTGTG
    GAGAAAAACC CAAATGCCGT GGTGACGCTA GAGAACTGGC AGTTTGAAGA ATGGGGAAGC
    CAAGTGACCG TAGTCTCATC AGACATGAGG GAATGGGTGG CTCCAGAGAA AGCAGACATC
    ATTGTCAGTG AGCTTCTGGG CTCATTTGCT GACAATGAAT TGTCGCCTGA GTGCCTGGAT
    GGAGCCCAGC ACTTCCTAAA AGATGATGGT GTGAGCATCC CCGGGGAGTA CACTTCCTTT
    CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC AATGAGGTCC GAGCCTGTAG GGAGAAGGAC
    CGTGACCCTG AGGCCCAGTT TGAGATGCCT TATGTGGTAC GGCTGCACAA CTTCCACCAG
    CTCTCTGCAC CCCAGCCCTG TTTCACCTTC AGCCATCCCA ACAGAGATCC TATGATTGAC
    AACAACCGCT ATTGCACCTT GGAATTTCCT GTGGAGGTGA ACACAGTACT ACATGGCTTT
    GCCGGCTACT TTGAGACTGT GCTTTATCAG GACATCACTC TGAGTATCCG TCCAGAGACT
    CACTCTCCTG GGATGTTCTC ATGGTTTCCC ATCCTCTTCC CTATTAAGCA GCCCATAACG
    GTACGTGAAG GCCAAACCAT CTGTGTGCGT TTCTGGCGAT GCAGCAATTC CAAGAAGGTG
    TGGTATGAGT GGGCTGTGAC AGCACCAGTC TGTTCTGCTA TTCATAACCC CACAGGCCGC
    TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269885.1
    CDS361..1761
    Translation

    Target ORF information:

    RefSeq Version NM_001282956.1
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGTTGGAG GTGCAGTTCA TCATCACAGG CACCAACCAC
    CACTCAGAGA AGGAGTTCTG CTCCTACCTC CAATACCTGG AATACTTAAG CCAGAACCGT
    CCTCCACCTA ATGCCTATGA ACTCTTTGCC AAGGGCTATG AAGACTATCT GCAGTCCCCG
    CTTCAGCCAC TGATGGACAA TCTGGAATCT CAGACATATG AAGTGTTTGA AAAGGACCCC
    ATCAAATACT CTCAGTACCA GCAGGCCATC TATAAATGTC TGCTAGACCG AGTACCAGAA
    GAGGAGAAGG ATACCAATGT CCAGGTACTG ATGGTGCTGG GAGCAGGACG GGGACCCCTG
    GTGAACGCTT CCCTGCGGGC AGCCAAGCAG GCCGACCGGC GGATAAAGCT GTATGCTGTG
    GAGAAAAACC CAAATGCCGT GGTGACGCTA GAGAACTGGC AGTTTGAAGA ATGGGGAAGC
    CAAGTGACCG TAGTCTCATC AGACATGAGG GAATGGGTGG CTCCAGAGAA AGCAGACATC
    ATTGTCAGTG AGCTTCTGGG CTCATTTGCT GACAATGAAT TGTCGCCTGA GTGCCTGGAT
    GGAGCCCAGC ACTTCCTAAA AGATGATGGT GTGAGCATCC CCGGGGAGTA CACTTCCTTT
    CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC AATGAGGTCC GAGCCTGTAG GGAGAAGGAC
    CGTGACCCTG AGGCCCAGTT TGAGATGCCT TATGTGGTAC GGCTGCACAA CTTCCACCAG
    CTCTCTGCAC CCCAGCCCTG TTTCACCTTC AGCCATCCCA ACAGAGATCC TATGATTGAC
    AACAACCGCT ATTGCACCTT GGAATTTCCT GTGGAGGTGA ACACAGTACT ACATGGCTTT
    GCCGGCTACT TTGAGACTGT GCTTTATCAG GACATCACTC TGAGTATCCG TCCAGAGACT
    CACTCTCCTG GGATGTTCTC ATGGTTTCCC ATCCTCTTCC CTATTAAGCA GCCCATAACG
    GTACGTGAAG GCCAAACCAT CTGTGTGCGT TTCTGGCGAT GCAGCAATTC CAAGAAGGTG
    TGGTATGAGT GGGCTGTGAC AGCACCAGTC TGTTCTGCTA TTCATAACCC CACAGGCCGC
    TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23914
    Clone ID Related Accession (Same CDS sequence) NM_001282956.1 , NM_001282956.2
    Accession Version NM_001282956.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, BC005820.2 and AL833612.1. On May 31, 2019 this sequence version replaced NM_001282956.1. Transcript Variant: This variant (6) contains multiple differences in the 5' coding region, compared to variant 1, including initiation of translation at an alternate start codon. The encoded isoform (f) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005820.2, SRR1803616.178198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGTTGGAG GTGCAGTTCA TCATCACAGG CACCAACCAC
    CACTCAGAGA AGGAGTTCTG CTCCTACCTC CAATACCTGG AATACTTAAG CCAGAACCGT
    CCTCCACCTA ATGCCTATGA ACTCTTTGCC AAGGGCTATG AAGACTATCT GCAGTCCCCG
    CTTCAGCCAC TGATGGACAA TCTGGAATCT CAGACATATG AAGTGTTTGA AAAGGACCCC
    ATCAAATACT CTCAGTACCA GCAGGCCATC TATAAATGTC TGCTAGACCG AGTACCAGAA
    GAGGAGAAGG ATACCAATGT CCAGGTACTG ATGGTGCTGG GAGCAGGACG GGGACCCCTG
    GTGAACGCTT CCCTGCGGGC AGCCAAGCAG GCCGACCGGC GGATAAAGCT GTATGCTGTG
    GAGAAAAACC CAAATGCCGT GGTGACGCTA GAGAACTGGC AGTTTGAAGA ATGGGGAAGC
    CAAGTGACCG TAGTCTCATC AGACATGAGG GAATGGGTGG CTCCAGAGAA AGCAGACATC
    ATTGTCAGTG AGCTTCTGGG CTCATTTGCT GACAATGAAT TGTCGCCTGA GTGCCTGGAT
    GGAGCCCAGC ACTTCCTAAA AGATGATGGT GTGAGCATCC CCGGGGAGTA CACTTCCTTT
    CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC AATGAGGTCC GAGCCTGTAG GGAGAAGGAC
    CGTGACCCTG AGGCCCAGTT TGAGATGCCT TATGTGGTAC GGCTGCACAA CTTCCACCAG
    CTCTCTGCAC CCCAGCCCTG TTTCACCTTC AGCCATCCCA ACAGAGATCC TATGATTGAC
    AACAACCGCT ATTGCACCTT GGAATTTCCT GTGGAGGTGA ACACAGTACT ACATGGCTTT
    GCCGGCTACT TTGAGACTGT GCTTTATCAG GACATCACTC TGAGTATCCG TCCAGAGACT
    CACTCTCCTG GGATGTTCTC ATGGTTTCCC ATCCTCTTCC CTATTAAGCA GCCCATAACG
    GTACGTGAAG GCCAAACCAT CTGTGTGCGT TTCTGGCGAT GCAGCAATTC CAAGAAGGTG
    TGGTATGAGT GGGCTGTGAC AGCACCAGTC TGTTCTGCTA TTCATAACCC CACAGGCCGC
    TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269885.1
    CDS152..1552
    Translation

    Target ORF information:

    RefSeq Version NM_001282956.2
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282956.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC
    TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG
    AAGATTCGCA GGAACTCCGA GGCGTTGGAG GTGCAGTTCA TCATCACAGG CACCAACCAC
    CACTCAGAGA AGGAGTTCTG CTCCTACCTC CAATACCTGG AATACTTAAG CCAGAACCGT
    CCTCCACCTA ATGCCTATGA ACTCTTTGCC AAGGGCTATG AAGACTATCT GCAGTCCCCG
    CTTCAGCCAC TGATGGACAA TCTGGAATCT CAGACATATG AAGTGTTTGA AAAGGACCCC
    ATCAAATACT CTCAGTACCA GCAGGCCATC TATAAATGTC TGCTAGACCG AGTACCAGAA
    GAGGAGAAGG ATACCAATGT CCAGGTACTG ATGGTGCTGG GAGCAGGACG GGGACCCCTG
    GTGAACGCTT CCCTGCGGGC AGCCAAGCAG GCCGACCGGC GGATAAAGCT GTATGCTGTG
    GAGAAAAACC CAAATGCCGT GGTGACGCTA GAGAACTGGC AGTTTGAAGA ATGGGGAAGC
    CAAGTGACCG TAGTCTCATC AGACATGAGG GAATGGGTGG CTCCAGAGAA AGCAGACATC
    ATTGTCAGTG AGCTTCTGGG CTCATTTGCT GACAATGAAT TGTCGCCTGA GTGCCTGGAT
    GGAGCCCAGC ACTTCCTAAA AGATGATGGT GTGAGCATCC CCGGGGAGTA CACTTCCTTT
    CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC AATGAGGTCC GAGCCTGTAG GGAGAAGGAC
    CGTGACCCTG AGGCCCAGTT TGAGATGCCT TATGTGGTAC GGCTGCACAA CTTCCACCAG
    CTCTCTGCAC CCCAGCCCTG TTTCACCTTC AGCCATCCCA ACAGAGATCC TATGATTGAC
    AACAACCGCT ATTGCACCTT GGAATTTCCT GTGGAGGTGA ACACAGTACT ACATGGCTTT
    GCCGGCTACT TTGAGACTGT GCTTTATCAG GACATCACTC TGAGTATCCG TCCAGAGACT
    CACTCTCCTG GGATGTTCTC ATGGTTTCCC ATCCTCTTCC CTATTAAGCA GCCCATAACG
    GTACGTGAAG GCCAAACCAT CTGTGTGCGT TTCTGGCGAT GCAGCAATTC CAAGAAGGTG
    TGGTATGAGT GGGCTGTGAC AGCACCAGTC TGTTCTGCTA TTCATAACCC CACAGGCCGC
    TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23924
    Clone ID Related Accession (Same CDS sequence) NM_001282953.1 , NM_001282953.2
    Accession Version NM_001282953.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-06
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395183.1, AK301812.1 and AA496357.1. On Sep 24, 2013 this sequence version replaced XM_005267260.1. Transcript Variant: This variant (3) contains multiple differences in the 5' coding region, compared to variant 1, including initiation of translation at an alternate start codon. The encoded isoform (c) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301812.1, SRR1803611.172563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGCT
    TTCCTGCTGC CCCTTAATCA GGAAGATAAC ACCAACCTGG CCAGAGTTTT GACCAACCAC
    ATCCACACTG GCCATCACTC TTCCATGTTC TGGATGCGGG TACCCTTGGT GGCACCAGAG
    GACCTGAGAG ATGATATAAT TGAGAATGCA CCAACTACAC ACACAGAGGA GTACAGTGGG
    GAGGAGAAAA CGTGGATGTG GTGGCACAAC TTCCGGACTT TGTGTGACTA TAGTAAGAGG
    ATTGCAGTGG CTCTTGAAAT TGGGGCTGAC CTCCCATCTA ATCATGTCAT TGATCGCTGG
    CTTGGGGAGC CCATCAAAGC AGCCATTCTC CCCACTAGCA TTTTCCTGAC CAATAAGAAG
    GGATTTCCTG TTCTTTCTAA GATGCACCAG AGGCTCATCT TCCGGCTCCT CAAGTTGGAG
    GTGCAGTTCA TCATCACAGG CACCAACCAC CACTCAGAGA AGGAGTTCTG CTCCTACCTC
    CAATACCTGG AATACTTAAG CCAGAACCGT CCTCCACCTA ATGCCTATGA ACTCTTTGCC
    AAGGGCTATG AAGACTATCT GCAGTCCCCG CTTCAGCCAC TGATGGACAA TCTGGAATCT
    CAGACATATG AAGTGTTTGA AAAGGACCCC ATCAAATACT CTCAGTACCA GCAGGCCATC
    TATAAATGTC TGCTAGACCG AGTACCAGAA GAGGAGAAGG ATACCAATGT CCAGGTACTG
    ATGGTGCTGG GAGCAGGACG GGGACCCCTG GTGAACGCTT CCCTGCGGGC AGCCAAGCAG
    GCCGACCGGC GGATAAAGCT GTATGCTGTG GAGAAAAACC CAAATGCCGT GGTGACGCTA
    GAGAACTGGC AGTTTGAAGA ATGGGGAAGC CAAGTGACCG TAGTCTCATC AGACATGAGG
    GAATGGGTGG CTCCAGAGAA AGCAGACATC ATTGTCAGTG AGCTTCTGGG CTCATTTGCT
    GACAATGAAT TGTCGCCTGA GTGCCTGGAT GGAGCCCAGC ACTTCCTAAA AGATGATGGT
    GTGAGCATCC CCGGGGAGTA CACTTCCTTT CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC
    AATGAGGTCC GAGCCTGTAG GGAGAAGGAC CGTGACCCTG AGGCCCAGTT TGAGATGCCT
    TATGTGGTAC GGCTGCACAA CTTCCACCAG CTCTCTGCAC CCCAGCCCTG TTTCACCTTC
    AGCCATCCCA ACAGAGATCC TATGATTGAC AACAACCGCT ATTGCACCTT GGAATTTCCT
    GTGGAGGTGA ACACAGTACT ACATGGCTTT GCCGGCTACT TTGAGACTGT GCTTTATCAG
    GACATCACTC TGAGTATCCG TCCAGAGACT CACTCTCCTG GGATGTTCTC ATGGTTTCCC
    ATCCTCTTCC CTATTAAGCA GCCCATAACG GTACGTGAAG GCCAAACCAT CTGTGTGCGT
    TTCTGGCGAT GCAGCAATTC CAAGAAGGTG TGGTATGAGT GGGCTGTGAC AGCACCAGTC
    TGTTCTGCTA TTCATAACCC CACAGGCCGC TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269882.1
    CDS361..2091
    Translation

    Target ORF information:

    RefSeq Version NM_001282953.1
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGCT
    TTCCTGCTGC CCCTTAATCA GGAAGATAAC ACCAACCTGG CCAGAGTTTT GACCAACCAC
    ATCCACACTG GCCATCACTC TTCCATGTTC TGGATGCGGG TACCCTTGGT GGCACCAGAG
    GACCTGAGAG ATGATATAAT TGAGAATGCA CCAACTACAC ACACAGAGGA GTACAGTGGG
    GAGGAGAAAA CGTGGATGTG GTGGCACAAC TTCCGGACTT TGTGTGACTA TAGTAAGAGG
    ATTGCAGTGG CTCTTGAAAT TGGGGCTGAC CTCCCATCTA ATCATGTCAT TGATCGCTGG
    CTTGGGGAGC CCATCAAAGC AGCCATTCTC CCCACTAGCA TTTTCCTGAC CAATAAGAAG
    GGATTTCCTG TTCTTTCTAA GATGCACCAG AGGCTCATCT TCCGGCTCCT CAAGTTGGAG
    GTGCAGTTCA TCATCACAGG CACCAACCAC CACTCAGAGA AGGAGTTCTG CTCCTACCTC
    CAATACCTGG AATACTTAAG CCAGAACCGT CCTCCACCTA ATGCCTATGA ACTCTTTGCC
    AAGGGCTATG AAGACTATCT GCAGTCCCCG CTTCAGCCAC TGATGGACAA TCTGGAATCT
    CAGACATATG AAGTGTTTGA AAAGGACCCC ATCAAATACT CTCAGTACCA GCAGGCCATC
    TATAAATGTC TGCTAGACCG AGTACCAGAA GAGGAGAAGG ATACCAATGT CCAGGTACTG
    ATGGTGCTGG GAGCAGGACG GGGACCCCTG GTGAACGCTT CCCTGCGGGC AGCCAAGCAG
    GCCGACCGGC GGATAAAGCT GTATGCTGTG GAGAAAAACC CAAATGCCGT GGTGACGCTA
    GAGAACTGGC AGTTTGAAGA ATGGGGAAGC CAAGTGACCG TAGTCTCATC AGACATGAGG
    GAATGGGTGG CTCCAGAGAA AGCAGACATC ATTGTCAGTG AGCTTCTGGG CTCATTTGCT
    GACAATGAAT TGTCGCCTGA GTGCCTGGAT GGAGCCCAGC ACTTCCTAAA AGATGATGGT
    GTGAGCATCC CCGGGGAGTA CACTTCCTTT CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC
    AATGAGGTCC GAGCCTGTAG GGAGAAGGAC CGTGACCCTG AGGCCCAGTT TGAGATGCCT
    TATGTGGTAC GGCTGCACAA CTTCCACCAG CTCTCTGCAC CCCAGCCCTG TTTCACCTTC
    AGCCATCCCA ACAGAGATCC TATGATTGAC AACAACCGCT ATTGCACCTT GGAATTTCCT
    GTGGAGGTGA ACACAGTACT ACATGGCTTT GCCGGCTACT TTGAGACTGT GCTTTATCAG
    GACATCACTC TGAGTATCCG TCCAGAGACT CACTCTCCTG GGATGTTCTC ATGGTTTCCC
    ATCCTCTTCC CTATTAAGCA GCCCATAACG GTACGTGAAG GCCAAACCAT CTGTGTGCGT
    TTCTGGCGAT GCAGCAATTC CAAGAAGGTG TGGTATGAGT GGGCTGTGAC AGCACCAGTC
    TGTTCTGCTA TTCATAACCC CACAGGCCGC TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23924
    Clone ID Related Accession (Same CDS sequence) NM_001282953.1 , NM_001282953.2
    Accession Version NM_001282953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product protein arginine N-methyltransferase 5 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK301812.1 and AA496357.1. On May 31, 2019 this sequence version replaced NM_001282953.1. Transcript Variant: This variant (3) contains multiple differences in the 5' coding region, compared to variant 1, including initiation of translation at an alternate start codon. The encoded isoform (c) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.172563.1, AK301812.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGCT
    TTCCTGCTGC CCCTTAATCA GGAAGATAAC ACCAACCTGG CCAGAGTTTT GACCAACCAC
    ATCCACACTG GCCATCACTC TTCCATGTTC TGGATGCGGG TACCCTTGGT GGCACCAGAG
    GACCTGAGAG ATGATATAAT TGAGAATGCA CCAACTACAC ACACAGAGGA GTACAGTGGG
    GAGGAGAAAA CGTGGATGTG GTGGCACAAC TTCCGGACTT TGTGTGACTA TAGTAAGAGG
    ATTGCAGTGG CTCTTGAAAT TGGGGCTGAC CTCCCATCTA ATCATGTCAT TGATCGCTGG
    CTTGGGGAGC CCATCAAAGC AGCCATTCTC CCCACTAGCA TTTTCCTGAC CAATAAGAAG
    GGATTTCCTG TTCTTTCTAA GATGCACCAG AGGCTCATCT TCCGGCTCCT CAAGTTGGAG
    GTGCAGTTCA TCATCACAGG CACCAACCAC CACTCAGAGA AGGAGTTCTG CTCCTACCTC
    CAATACCTGG AATACTTAAG CCAGAACCGT CCTCCACCTA ATGCCTATGA ACTCTTTGCC
    AAGGGCTATG AAGACTATCT GCAGTCCCCG CTTCAGCCAC TGATGGACAA TCTGGAATCT
    CAGACATATG AAGTGTTTGA AAAGGACCCC ATCAAATACT CTCAGTACCA GCAGGCCATC
    TATAAATGTC TGCTAGACCG AGTACCAGAA GAGGAGAAGG ATACCAATGT CCAGGTACTG
    ATGGTGCTGG GAGCAGGACG GGGACCCCTG GTGAACGCTT CCCTGCGGGC AGCCAAGCAG
    GCCGACCGGC GGATAAAGCT GTATGCTGTG GAGAAAAACC CAAATGCCGT GGTGACGCTA
    GAGAACTGGC AGTTTGAAGA ATGGGGAAGC CAAGTGACCG TAGTCTCATC AGACATGAGG
    GAATGGGTGG CTCCAGAGAA AGCAGACATC ATTGTCAGTG AGCTTCTGGG CTCATTTGCT
    GACAATGAAT TGTCGCCTGA GTGCCTGGAT GGAGCCCAGC ACTTCCTAAA AGATGATGGT
    GTGAGCATCC CCGGGGAGTA CACTTCCTTT CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC
    AATGAGGTCC GAGCCTGTAG GGAGAAGGAC CGTGACCCTG AGGCCCAGTT TGAGATGCCT
    TATGTGGTAC GGCTGCACAA CTTCCACCAG CTCTCTGCAC CCCAGCCCTG TTTCACCTTC
    AGCCATCCCA ACAGAGATCC TATGATTGAC AACAACCGCT ATTGCACCTT GGAATTTCCT
    GTGGAGGTGA ACACAGTACT ACATGGCTTT GCCGGCTACT TTGAGACTGT GCTTTATCAG
    GACATCACTC TGAGTATCCG TCCAGAGACT CACTCTCCTG GGATGTTCTC ATGGTTTCCC
    ATCCTCTTCC CTATTAAGCA GCCCATAACG GTACGTGAAG GCCAAACCAT CTGTGTGCGT
    TTCTGGCGAT GCAGCAATTC CAAGAAGGTG TGGTATGAGT GGGCTGTGAC AGCACCAGTC
    TGTTCTGCTA TTCATAACCC CACAGGCCGC TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269882.1
    CDS152..1882
    Translation

    Target ORF information:

    RefSeq Version NM_001282953.2
    Organism Homo sapiens(human)
    Definition Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCGGGGTC CGAACTCGGG GACGGAGAAG GGCAGACTAG TCATCCCGGA GAAGCAGGGG 
    TTTGATTTCC TCTGCATGCC TGTCTTCCAT CCGCGTTTCA AGAGGGAGTT CATTCAGGAA
    CCTGCTAAGA ATCGGCCCGG TCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGCT
    TTCCTGCTGC CCCTTAATCA GGAAGATAAC ACCAACCTGG CCAGAGTTTT GACCAACCAC
    ATCCACACTG GCCATCACTC TTCCATGTTC TGGATGCGGG TACCCTTGGT GGCACCAGAG
    GACCTGAGAG ATGATATAAT TGAGAATGCA CCAACTACAC ACACAGAGGA GTACAGTGGG
    GAGGAGAAAA CGTGGATGTG GTGGCACAAC TTCCGGACTT TGTGTGACTA TAGTAAGAGG
    ATTGCAGTGG CTCTTGAAAT TGGGGCTGAC CTCCCATCTA ATCATGTCAT TGATCGCTGG
    CTTGGGGAGC CCATCAAAGC AGCCATTCTC CCCACTAGCA TTTTCCTGAC CAATAAGAAG
    GGATTTCCTG TTCTTTCTAA GATGCACCAG AGGCTCATCT TCCGGCTCCT CAAGTTGGAG
    GTGCAGTTCA TCATCACAGG CACCAACCAC CACTCAGAGA AGGAGTTCTG CTCCTACCTC
    CAATACCTGG AATACTTAAG CCAGAACCGT CCTCCACCTA ATGCCTATGA ACTCTTTGCC
    AAGGGCTATG AAGACTATCT GCAGTCCCCG CTTCAGCCAC TGATGGACAA TCTGGAATCT
    CAGACATATG AAGTGTTTGA AAAGGACCCC ATCAAATACT CTCAGTACCA GCAGGCCATC
    TATAAATGTC TGCTAGACCG AGTACCAGAA GAGGAGAAGG ATACCAATGT CCAGGTACTG
    ATGGTGCTGG GAGCAGGACG GGGACCCCTG GTGAACGCTT CCCTGCGGGC AGCCAAGCAG
    GCCGACCGGC GGATAAAGCT GTATGCTGTG GAGAAAAACC CAAATGCCGT GGTGACGCTA
    GAGAACTGGC AGTTTGAAGA ATGGGGAAGC CAAGTGACCG TAGTCTCATC AGACATGAGG
    GAATGGGTGG CTCCAGAGAA AGCAGACATC ATTGTCAGTG AGCTTCTGGG CTCATTTGCT
    GACAATGAAT TGTCGCCTGA GTGCCTGGAT GGAGCCCAGC ACTTCCTAAA AGATGATGGT
    GTGAGCATCC CCGGGGAGTA CACTTCCTTT CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC
    AATGAGGTCC GAGCCTGTAG GGAGAAGGAC CGTGACCCTG AGGCCCAGTT TGAGATGCCT
    TATGTGGTAC GGCTGCACAA CTTCCACCAG CTCTCTGCAC CCCAGCCCTG TTTCACCTTC
    AGCCATCCCA ACAGAGATCC TATGATTGAC AACAACCGCT ATTGCACCTT GGAATTTCCT
    GTGGAGGTGA ACACAGTACT ACATGGCTTT GCCGGCTACT TTGAGACTGT GCTTTATCAG
    GACATCACTC TGAGTATCCG TCCAGAGACT CACTCTCCTG GGATGTTCTC ATGGTTTCCC
    ATCCTCTTCC CTATTAAGCA GCCCATAACG GTACGTGAAG GCCAAACCAT CTGTGTGCGT
    TTCTGGCGAT GCAGCAATTC CAAGAAGGTG TGGTATGAGT GGGCTGTGAC AGCACCAGTC
    TGTTCTGCTA TTCATAACCC CACAGGCCGC TCATATACCA TTGGCCTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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